<?xml version="1.0" encoding="UTF-8"?>
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 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
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<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHS2</accession>
    <name>H4GHS2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycerol-3-phosphate acyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Acyl-PO4 G3P acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Acyl-phosphate--glycerol-3-phosphate acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">G3P acyltransferase</fullName>
            <shortName evidence="10">GPAT</shortName>
            <ecNumber evidence="10">2.3.1.275</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Lysophosphatidic acid synthase</fullName>
            <shortName evidence="10">LPA synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">plsY</name>
        <name evidence="11" type="ORF">PS3_16607</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the transfer of an acyl group from acyl-phosphate (acyl-PO(4)) to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme utilizes acyl-phosphate as fatty acyl donor, but not acyl-CoA or acyl-ACP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an acyl phosphate + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:34075"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57970"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="2.3.1.275"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Probably interacts with PlsX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the PlsY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.275" evidence="10"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16607"/>
    <dbReference type="EnsemblBacteria" id="EHS87478">
        <property type="protein sequence ID" value="EHS87478"/>
        <property type="gene ID" value="PS3_16607"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.40"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043772">
        <property type="term" value="F:acyl-phosphate glycerol-3-phosphate acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01043">
        <property type="entry name" value="PlsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003811">
        <property type="entry name" value="G3P_acylTferase_PlsY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30309">
        <property type="entry name" value="PTHR30309"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02660">
        <property type="entry name" value="G3P_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01207">
        <property type="entry name" value="G3P_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00023">
        <property type="entry name" value="TIGR00023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="159"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="206" mass="22630" checksum="09DDDA37E2C2CFC7" modified="2012-04-18" version="1">MILTLVMILIAYLLGSIPSGLWIGLYFYHKDLRRYGSGNIGTTNTFRILGNKAGTIVFIMDVLKGSLATCLPYLFHSPINPLFIGFFAAVGHVFSIFDHFKGGKAVATSSGVLLAYNPLLFLVGVVIFTITLLLTSMVSVGSLVVVLSITIIALIQHDWILSLLGIVITIGVYYLHRSNIQRIIHGQESMVSFGLGQYLRKKHSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHS8</accession>
    <name>H4GHS8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">Ribosome biogenesis GTPase A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_7153</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for a late step of 50S ribosomal subunit assembly. Has GTPase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class YlqF/YawG GTPase family. MTG1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121612.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7153"/>
    <dbReference type="EnsemblBacteria" id="EHS87484">
        <property type="protein sequence ID" value="EHS87484"/>
        <property type="gene ID" value="PS3_7153"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.47"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019991">
        <property type="entry name" value="GTP-bd_ribosome_bgen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016478">
        <property type="entry name" value="GTPase_MTG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006230">
        <property type="entry name" value="MG442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03596">
        <property type="entry name" value="GTPase_YlqF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="4">
        <location>
            <begin position="15"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="131"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR006230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR006230-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="290" mass="32352" checksum="B94AB20775896D1E" modified="2012-04-18" version="1">MAVIQWFPGHMAKALRQIREQMPLVDLVFELVDARVPYSSQNPEVDLAAGDKPRLLIMTKTDLADPEVTKEWLAFFKNQGQPVLALDSREQQVAKKVTNAAKKVLADKMANEAAKGMQKRAIKAMCVGVPNVGKSTLLNHLVHKNVAATANRPGVTTGQQWLRSSAELELLDTPGVLWPKFATTKQGTMLALSGAIKDSLYPKDDVAIFALDYLRQYRMAELKKRYRLTDGEVDEAVSTPELLLTITQKLGMRDDYERASERIIFDMRKGKLGTFTLERPADLNAEGLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHV7</accession>
    <name>H4GHV7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Potassium transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_15786</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121641.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15786"/>
    <dbReference type="EnsemblBacteria" id="EHS87513">
        <property type="protein sequence ID" value="EHS87513"/>
        <property type="gene ID" value="PS3_15786"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.82"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="347"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="377"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="447" mass="49359" checksum="B24C95B554C64068" modified="2012-04-18" version="1">MIEINKHRYSFPEILTIGFIVVIIVGTFLLKLPIATRAGLETTWMTAIFTSTSATCVTGLLLVDTAAHWTWFGQLVITILMEVGGLGFMTFAVMGFLLIRRRMRLSTQLLTQEALNLDHLSQIRVVYLIIKLSLLIQGIGAIFLTFDLVPKYGFKRGLWLSVFQAISAFCNAGFDLFGNSLSDFKNDPYFLIVTAILIIAGSFGFLVWQDIVEYRRFHRMSLHTKLALRTGGVLVFLSILVYLITEKNLSQFANEMSPINRFINTIFMAITPRTAGLTTIPYDHLSSAGISYTTILMFIGGAPGSTAGGVKTTTIGLIALQSIATLRGKSDTVMAHRRFTQENIFRALTLLFISVVLILSAVLLLVATQALPVKDPLSYVLFEAVAAFGTTGISLGITPTLNAIGKIIIMLLMFVGRVGIYTVMFSIFNAKPEKQTYRYPEESVLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHV8</accession>
    <name>H4GHV8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase</fullName>
            <ecNumber evidence="2">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_10155</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121642.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10155"/>
    <dbReference type="EnsemblBacteria" id="EHS87514">
        <property type="protein sequence ID" value="EHS87514"/>
        <property type="gene ID" value="PS3_10155"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.83"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="4">
        <location>
            <begin position="37"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="393" mass="42793" checksum="7385A838F87D2D43" modified="2012-04-18" version="1">MPETLATLTNLRNQLLDTVAPSAIRSFDNQISSIPGIIKLTLGEPDFNVPEHVKAAAIKSIEENDSHYAPASGKPELRQAISDYLKDSRGVTYNPADEVLVTVGATESLASITYGILNPGDKVLVPTPAFSLYFALVKMAGAEPVMIDTSNDGFQLTAQRLEAEIEAAGDQVKAVLLNYPNNPTGRTFTKTELEDLAAVLKNHELLVIADEIYSDLTYDQDHYSLATLLPEQTLLVSGLSKSHAMTGYRMGYVAGPAGIMRSVAQIHSYLVVCVNDATQAAAVEALNNGREDPLVFKKAYKKRRDYIIEKMTKMGFQIATPQGAFYVFAKIPDQFGKDDFKFALDVAKQAQVGLIPGSVFGAGSDGYVRLSYAASDENIKVAMERLATYMEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHW1</accession>
    <name>H4GHW1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyldiaminopimelate deacetylase</fullName>
            <ecNumber evidence="1">3.5.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_13149</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of N-acetyl-diaminopimelate to diaminopimelate and acetate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + N-acetyl-(2S,6S)-2,6-diaminoheptanedioate = (2S,6S)-2,6-diaminoheptanedioate + acetate</text>
            <dbReference type="Rhea" id="RHEA:20405"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:58767"/>
            <dbReference type="EC" id="3.5.1.47"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (acetylase route): step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family. N-acetyldiaminopimelate deacetylase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.47" evidence="1"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121645.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13149"/>
    <dbReference type="EnsemblBacteria" id="EHS87517">
        <property type="protein sequence ID" value="EHS87517"/>
        <property type="gene ID" value="PS3_13149"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.86"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00024"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050118">
        <property type="term" value="F:N-acetyldiaminopimelate deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01692">
        <property type="entry name" value="DapEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023905">
        <property type="entry name" value="AcetylDAP_deacetylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PTHR11014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014:SF98">
        <property type="entry name" value="PTHR11014:SF98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="1" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="2">
        <location>
            <begin position="181"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="389" mass="43148" checksum="15282BF43CBC19D1" modified="2012-04-18" version="1">MSQLTHDELIQIRRHLHQIPELALEEYQTHDYLLEVIKQFDQTYLTIRVPKELPTAIMVQVAGTNPRRTIGYRTDIDALPVTEQTGLPYASQTPGEMHACGHDIHMTVALGLLSYYSEHQPTDNLLFFFQPAEESKNGGKLAYEFGLFTGDWCPDEFYGLHDNPELPGGAIGCRMGTLFAGTTEVNIDIQGQGGHAAFPQNAKDAVVAAASLITQAQTIVSRSIDPIQSGVLTLGKLEAGTIRNVIADHARIEGTIRGLTQPMIEKIGQRLQTICDGIATSFEVDVKLELNQGGYWPVENNDRLTSHLIAFMQANSKINFIETAPAMTGEDFGFLLAKFPGTMFWLGVEDDAQLHAPTLTPDERAIDKGIEAITQFINYRMQENYLLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHX2</accession>
    <name>H4GHX2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">DNA repair protein RecO</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Recombination protein O</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">recO</name>
        <name evidence="9" type="ORF">PS3_8064</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in DNA repair and RecF pathway recombination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the RecO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8064"/>
    <dbReference type="EnsemblBacteria" id="EHS87528">
        <property type="protein sequence ID" value="EHS87528"/>
        <property type="gene ID" value="PS3_8064"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.98"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00201">
        <property type="entry name" value="RecO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022572">
        <property type="entry name" value="DNA_rep/recomb_RecO_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003717">
        <property type="entry name" value="RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042242">
        <property type="entry name" value="RecO_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33991">
        <property type="entry name" value="PTHR33991"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02565">
        <property type="entry name" value="RecO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11967">
        <property type="entry name" value="RecO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="SSF57863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00613">
        <property type="entry name" value="reco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_rep/recomb_RecO_N" evidence="8">
        <location>
            <begin position="7"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="264" mass="30529" checksum="B9991335D62EABE4" modified="2012-04-18" version="1">MRVNEPFQGIIMNRRPYRERDMLVKILTDQRGPVMFFVRGAQRKGFKMAADILPFTYGQYVGLFNDDGLSYIVSAGETHQYQGIVNDLDRNAYATYLLDLVNQAFKEGFGIGGWYQQISTALDLINQGLDPQIITNVLEVQLLNLLGVAPQWDRCVICGNDQAPFDYSESYGGILCRQHWALDEHRFRLDQRTISYLRLFAHLNLTKVGQVSVADPIRKQLTWALDKIYDDQVGMRLKSKRFLREMDHWTAELKKSRDQIDKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHZ1</accession>
    <name>H4GHZ1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Phosphoenolpyruvate-dependent sugar phosphotransferase system EIIC.1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PS3_4914</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000006">
        <property type="protein sequence ID" value="EHS87470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121675.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000006.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4914"/>
    <dbReference type="EnsemblBacteria" id="EHS87470">
        <property type="protein sequence ID" value="EHS87470"/>
        <property type="gene ID" value="PS3_4914"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.120"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015577">
        <property type="term" value="F:galactitol transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013853">
        <property type="entry name" value="GatC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013014">
        <property type="entry name" value="PTS_EIIC_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004703">
        <property type="entry name" value="PTS_sugar-sp_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37324">
        <property type="entry name" value="PTHR37324"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03611">
        <property type="entry name" value="EIIC-GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006304">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51104">
        <property type="entry name" value="PTS_EIIC_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="137"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIC type-2" evidence="10">
        <location>
            <begin position="8"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="450" mass="47972" checksum="C2641ED93E5F55EC" modified="2012-04-18" version="1">MEMIAAGLQWFVNLGASVMLPILLFIFALILRIKPGKAFKAGLTVGIGFIGLNLVITLLTENLGPAAHTMVRNFGLHLGTLDIGWPAASAVAYGTVLGSLAIPVGVIANLVLLLFGLTKTLDVDVWNYWHIAFTGSIVYAATGDFILGIFTMVVHVMIIYLLADLSAPYIQKQYGLEGISFPQGASAPGFILALPLNWIMDRIPGINKIKLTPQTIQKRMGVFGDSGVMGLIIGIVIGALAKYSLAKTLQLGVTTAAVLILMPRMVSLLMEGLTPISEGANDLVKKHFPGRDLYIGMDSALAVGNETVLSSALILVPVTLLIAIILPGNSMLQFGDLATIPYMIAIMCAVFRGDIFRSLIGGIVDIVISLYIASWVAPLVTASAKAAKFSLHGSSSISVLSDGGVWTTWVIVGLGKVMTWGGIGLIGLITLGLMIWFNKFHNRKRIKQDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHZ4</accession>
    <name>H4GHZ4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dihydroorotate dehydrogenase</fullName>
            <shortName evidence="9">DHOD</shortName>
            <shortName evidence="9">DHODase</shortName>
            <shortName evidence="9">DHOdehase</shortName>
            <ecNumber evidence="9">1.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pyrD</name>
        <name evidence="11" type="ORF">PS3_5726</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the conversion of dihydroorotate to orotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(S)-dihydroorotate + A = AH2 + orotate</text>
            <dbReference type="Rhea" id="RHEA:18073"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="9">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Pyrimidine metabolism; UMP biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.-.-" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000006">
        <property type="protein sequence ID" value="EHS87473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121678.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000006.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5726"/>
    <dbReference type="EnsemblBacteria" id="EHS87473">
        <property type="protein sequence ID" value="EHS87473"/>
        <property type="gene ID" value="PS3_5726"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.124"/>
    <dbReference type="UniPathway" id="UPA00070"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04740">
        <property type="entry name" value="DHOD_1B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00224">
        <property type="entry name" value="DHO_dh_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033888">
        <property type="entry name" value="DHOD_1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024920">
        <property type="entry name" value="Dihydroorotate_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012135">
        <property type="entry name" value="Dihydroorotate_DH_1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000164">
        <property type="entry name" value="DHO_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01037">
        <property type="entry name" value="pyrD_sub1_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 9" id="KW-0288">FMN</keyword>
    <keyword evidence="8 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHO_dh" evidence="10">
        <location>
            <begin position="5"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="71"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="197"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="47"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="248"/>
            <end position="249"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="270"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="306" mass="32077" checksum="060D2AA471D214D0" modified="2012-04-18" version="1">MSNRLAINIPGLSMKNPLMPASGTFGFGDVAGASKFDLNQMGAMVMKTTTPQATTGNSQPQIAQLDDGVLNSVGLTNPGVEAVINDKMFNFHQQYPVLPIVASIGGDTESDYVEVAQKLAQSGLANALELNVSCPNVASGGMSFGVDPKTVESLTSQVKAVTGNVPVYVKLTPNVTDITEIARAAEDGGADGLSLINTVLGLHIDIRTRKAVLGKGMGGFSGHAVKPIAVRMVYQVRQVTNLPIIGMGGVETPADVIEFFLAGADAIAVGTAHFKHWDAIPRIAKGLVPLMDQLGIASLEELRQQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHZ5</accession>
    <name>H4GHZ5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Carbamoyl-phosphate synthase large chain</fullName>
            <ecNumber evidence="13">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Carbamoyl-phosphate synthetase ammonia chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">carB</name>
        <name evidence="17" type="ORF">PS3_4943</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="13">Binds 4 Mg(2+) or Mn(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the CarB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="13"/>
    <dbReference type="EMBL" id="AICN01000006">
        <property type="protein sequence ID" value="EHS87474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121679.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000006.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4943"/>
    <dbReference type="EnsemblBacteria" id="EHS87474">
        <property type="protein sequence ID" value="EHS87474"/>
        <property type="gene ID" value="PS3_4943"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.125"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01424">
        <property type="entry name" value="MGS_CPS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01210_A">
        <property type="entry name" value="CPSase_L_chain_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01210_B">
        <property type="entry name" value="CPSase_L_chain_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CarbamoylP_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CbamoylP_synth_lsu_CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033937">
        <property type="entry name" value="MGS_CPS_CarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="SSF48108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="9 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 13" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-grasp" evidence="15">
        <location>
            <begin position="133"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="15">
        <location>
            <begin position="671"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="16">
        <location>
            <begin position="930"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carboxyphosphate synthetic domain" evidence="13">
        <location>
            <begin position="1"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate synthetic domain" evidence="13">
        <location>
            <begin position="547"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Allosteric domain" evidence="13">
        <location>
            <begin position="930"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="820"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="832"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="834"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EHS87474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="1059" mass="116349" checksum="F269E2162018607F" modified="2012-04-18" version="1">MPKRTDIHKIMVIGSGPIIIGQAAEFDYSGTQACMALREEGYETVLVNSNPATIMTDNEIADHVYIEPLTVESVSRIIRQEFPDAILPTLGGQIGLNLAVALADTGILDELGIQLLGTQLASIDQAEDREKFKQLMQDLNEPIPPSKTVETVDGAVDFANEIGYPVIVRPAFTMGGTGGGMCDNEEELREIAKNGLELSPATQCLIEKSIAGYKEVEFEVMRDSADNAMVVCCMENFDPVGIHTGDSIVIAPCQTLSDREYQMLRNCALKLIRALKIEGGCNVQLALDPNSYHYNVIEVNPRVSRSSALASKATGYPIAKMAAKIAVGLTLDEIKNPVTETTYAEFEPALDYVVAKIPRWPFDKFVHADRRLGSQMKATGEVMAIGRNLEEALLKAVRSMEIDQADLAYPTAQAASDADLEMQLVHVQDDRLFYLAEAFRRGYSIDELFQLTKITPYFLDIVKHLVELETTVADHVGDVASLKLAKRYGFSDPTIARLWGQTSDEVRQLRLANGIVPVYKMVDTCAAEFDSQTPYFYSTYAQENESHQSDRQSVMVIGSGPIRIGQGVEFDYATVHCVRALQRLGYEAIVMNSNPETVSTDFSISDKLYFEPLTLEDVLNVIDLEQPVGVIVQFGGQTAINLAAGLDAHGVKVLGTTVKDLDAAEDREVFDRVIKDLGLKQPIGMTATTHQGVIDAAAEIGYPVLVRPSYVLGGKAMEIVYNQDELEEYLAQNETVAEEHPILVDSYLVGRECEVDAICDGHQVLLPGIMEHIERAGVHSGDSMAVYPPQNISEDVKAQIVAATEKLAVALKCIGIMNIQFIIHDGEAYILEVNPRASRTVPFLSKITGVEMAQVATRVIMGESLAEQGFAPGLYPESHMVHVKAPVFSFSKLGDVDSYLGPEMKSTGEVMGTDSTFEKALYKAFARAKMTLPDNGSVLLTIEDNDKDEILPLAKRFAKIGYRLMATEGTADFLRNNGLHTTVVHKIHDQREDNIMNLLLNDQVDLVINTMGHDYEQNSDGFIIRQTAIEHHIPVVTPLDTVDALLRALESRAFTTVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI07</accession>
    <name>H4GI07_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha/beta fold family hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_9427</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000007">
        <property type="protein sequence ID" value="EHS87458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121691.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000007.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9427"/>
    <dbReference type="EnsemblBacteria" id="EHS87458">
        <property type="protein sequence ID" value="EHS87458"/>
        <property type="gene ID" value="PS3_9427"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.140"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="2">
        <location>
            <begin position="25"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="250" mass="27695" checksum="8E29129FDB92ECC1" modified="2012-04-18" version="1">MKIEIQRDGLTLRGELSGTDTLKNETLVVMMHGFKGSIGYDVANLLFDTHNQLNLHGLPTLRINFNGCGDSDGEFKDMTVLNEIQDGIAILEYVQKVIQPRHLYLVGHSQGGVVASMLAAYYHDQIEKLVLLAPAATLVSDAQAGICQGVSYDPQNVPDSIMLDGFEVGGAYFRTAQHLHIYETAQGYQGPVLLVHGLDDQVVSYEATKKYDQIYQNDQMHLIAGASHNLMGDQDQRQVVKDLVTRFLME</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI21</accession>
    <name>H4GI21_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate-specific transport system accessory protein PhoU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_3002</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the regulation of phosphate uptake.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PhoU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000011">
        <property type="protein sequence ID" value="EHS87431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121705.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000011.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3002"/>
    <dbReference type="EnsemblBacteria" id="EHS87431">
        <property type="protein sequence ID" value="EHS87431"/>
        <property type="gene ID" value="PS3_3002"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.160"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:cellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="P_transport_PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PTHR42930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003107">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02135">
        <property type="entry name" value="phoU_full"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PhoU" evidence="2">
        <location>
            <begin position="18"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="2">
        <location>
            <begin position="125"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR003107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="237" mass="26834" checksum="67220579DFCFE390" modified="2012-04-18" version="1">MSEIFKDELKKLRIHFVEMGINVNEQIYRATKAFTSHDRALADQVLELDRQINDQEVTLEKQALKLMALQQPLANDFRLIISILKASNDIERLGDYASHIARSAKHVETADTDQAVDQAIDATTNTIRQMLEKVLDAFVNDNERAAYEVAEEDLAVDVVHVQQQKQILKKMTENQAAIPSYEAYLAVLRNLERAGDHIVNLAEWVVYIQTGKLVELNPGKTDPKLVKEALAKVKSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI23</accession>
    <name>H4GI23_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphate transport system permease protein PstA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_14259</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000011">
        <property type="protein sequence ID" value="EHS87433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121707.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000011.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14259"/>
    <dbReference type="EnsemblBacteria" id="EHS87433">
        <property type="protein sequence ID" value="EHS87433"/>
        <property type="gene ID" value="PS3_14259"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.162"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005672">
        <property type="entry name" value="Phosphate_PstA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00974">
        <property type="entry name" value="3a0107s02c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="62"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="291" mass="31092" checksum="9D21164B296DDD22" modified="2012-04-18" version="1">MKKIQVDRLMTGIILFGAGIAGLLIISLIGYILVSGVESLSWRFITTVSSSFSAGGGIRDQIFNSLYLVVLTMILALPVSLGGAIYLNEYAKGSRLAAGLRMLIDALSSLPSIVVGLFGYLVLVVQLHLGFSLLAGAVVLTIINLPILTRSIEQALINVPDLQRQAGLGLGMSRWKVITKIVLPMAIPEIVSGAILAIGRIFGEAAALIYTAGQSSINISYSDWNPFSPTSFLNPLRPAETLAVHIWKLNTEGLVPDAISVSAGAAVVLIILIVIFNLLARWLGKWLANRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI67</accession>
    <name>H4GI67_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribosome biogenesis GTPase YqeH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_11365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121751.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11365"/>
    <dbReference type="EnsemblBacteria" id="EHS87406">
        <property type="protein sequence ID" value="EHS87406"/>
        <property type="gene ID" value="PS3_11365"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.220"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019988">
        <property type="entry name" value="GTP-bd_ribosome_bgen_YqeH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03597">
        <property type="entry name" value="GTPase_YqeH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="1">
        <location>
            <begin position="67"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="372" mass="41607" checksum="A7E79EA3E1206A6B" modified="2012-04-18" version="1">MATVDDELRCIGCGAVIQSDHPDELGYTPASAVQKGLESGELYCQRCFRLRHYNEIAPVSLTDDDFLRLLSHIRDTKSLIVYVVDIFDVNGSLIPGLHRFVGDNPVMLVGNKEDLLPSSLRRNKLRDWLRQQVNQAGIRPISTVLVSAKKNHQIEDLLKEIDRRRNDRDVYVVGVTNVGKSTLINQIIKLRTGIKDLITTSRFPGTTLDQIEIPLDDGHQLVDTPGIIHQAQMAHVLPAKDLKVVTPQKEIKPRTYQLQDQQTIFLGGVARFDYLKGPKVGIVGYFDNNLPMHRTKLAHADDFYHKHLGGLLTPPSTDDLADFPPLERYEFAIKQKSDLVFEGLGWMTIPAGTVVAGWAPKGVAVLIRPAMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI68</accession>
    <name>H4GI68_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HAD superfamily (Subfamily IIIA) phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_5390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121752.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5390"/>
    <dbReference type="EnsemblBacteria" id="EHS87407">
        <property type="protein sequence ID" value="EHS87407"/>
        <property type="gene ID" value="PS3_5390"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.221"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010021">
        <property type="entry name" value="PGPP1/Gep4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF25">
        <property type="entry name" value="PTHR19288:SF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01668">
        <property type="entry name" value="YqeG_hyp_ppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="175" mass="19982" checksum="148ACCFF8D008179" modified="2012-04-18" version="1">MLARFKPTWMIERFSDIDPDQLKQMGIHVVFSDLDNTLIAWNNPDGTPELREWMRKLADAQIQLIVVSNNSHDRIGRAVEKIGLPFVSRALKPLPVGINRTLKKMQLAPSEVVMVGDQIMTDILAANGAGIRSILVKPLLPSDKWITKPNRMMEKQVMRLLQGQDSQMQWQKELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI81</accession>
    <name>H4GI81_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_7297</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121765.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7297"/>
    <dbReference type="EnsemblBacteria" id="EHS87420">
        <property type="protein sequence ID" value="EHS87420"/>
        <property type="gene ID" value="PS3_7297"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.237"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010288">
        <property type="entry name" value="EcsB_ABC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05975">
        <property type="entry name" value="EcsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037259">
        <property type="entry name" value="EcsB_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="279"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="349"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="394" mass="46205" checksum="592403F75AC09FF6" modified="2012-04-18" version="1">MLNKLFWQRQQSRWVHYLHYWRLVFNDHFIIALFFMAGAMAYSYDQLLPQVNSQPWWRLILIGWVTLAMQVGRWALFVEQPDPVVLLPLTSQIKQYLKPASYYSASLGLIMTLALTVIALPLALTIMKISTLTMIVMGLTVVTLKLNWLVWEKQRFLNFTKNTRLINFVQWGMTLVIAAIMWMEWPLMAWIFSLMLLLTGQYLNSRTSFDWSAAIAYEQARTESIYRFFNLFTDVPQVQGRVKRRAWADGIIKYLSHQDGQWGYLYARGFVRGSDTSGLVVRLTLILAIITFFIPINWLQTVVLALSLYVIVSQLGPEYHQFDNKVFTYLYPLEVKQRQNAYLSLTRRVLWPVAIILILATVMSSYLILNVFIIIIEGELLVHWYLPMRVFKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI91</accession>
    <name>H4GI91_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Magnesium transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_17526</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121775.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17526"/>
    <dbReference type="EnsemblBacteria" id="EHS87312">
        <property type="protein sequence ID" value="EHS87312"/>
        <property type="gene ID" value="PS3_17526"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.253"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="SSF143865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="SSF144083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="306" mass="35518" checksum="548B1510471C118B" modified="2012-04-18" version="1">MLSSVQLNEHSYWVSVFEPLPNEQQELFERYEVTSDMLDYAIDPFEKARIEIDHDAHITLLIFDVYIAHPEEDDDVTAPIGIMLTNNNVLTFTNAQTNFVNGIIAQLIKNERAHHPIHSIYDVVLPLLYQLSTDYFLPIRKADNQRKALQKNIRKRNERVSINKFMELETNLVYILSSIQGNVSLLQEFKRRFLYTLTTSQQNKLDDAIIEAQQGLEMAQMTSDVVSRVSSAYSNVLDSNLNQTMKFLTVYSIILAIPPIVSGFYGENVKSLPFAQNDWAWQITVIITLIFIALSIWFMFNKRWWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIB2</accession>
    <name>H4GIB2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">UspA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_10163</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87333">
        <property type="protein sequence ID" value="EHS87333"/>
        <property type="gene ID" value="PS3_10163"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.278"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006276">
        <property type="entry name" value="UspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="3">
        <location>
            <begin position="12"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="174" mass="19039" checksum="0DCC1CAAEF0E6366" modified="2012-04-18" version="1">MWRGNIMQLKEYHHILVPVDGSKATEAVVAQAAVMAKAHHANLELLNVVQVAQFSDGIISGISLDKDQTYNLINQANERLQDLKKELEATGVEKVGVHVRFGNPKQVIAHDFVEDHQIDLIVMGATGLSAVERMVVGSVTQYATRNADADVLVVKINKDDFKVKSSSDEPIVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIB4</accession>
    <name>H4GIB4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 15">Phosphoenolpyruvate-protein phosphotransferase</fullName>
            <ecNumber evidence="6 15">2.7.3.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14 15">Phosphotransferase system, enzyme I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PS3_10999</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 15">General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-L-histidyl-[protein] + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:23880"/>
            <dbReference type="Rhea" id="RHEA-COMP:9745"/>
            <dbReference type="Rhea" id="RHEA-COMP:9746"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:64837"/>
            <dbReference type="EC" id="2.7.3.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15 18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.3.9" evidence="6 15"/>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121798.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10999"/>
    <dbReference type="EnsemblBacteria" id="EHS87335">
        <property type="protein sequence ID" value="EHS87335"/>
        <property type="gene ID" value="PS3_10999"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.283"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008965">
        <property type="term" value="F:phosphoenolpyruvate-protein phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018274">
        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024692">
        <property type="entry name" value="PTS_EI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006318">
        <property type="entry name" value="PTS_EI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008731">
        <property type="entry name" value="PTS_EIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036618">
        <property type="entry name" value="PtsI_HPr-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05524">
        <property type="entry name" value="PEP-utilisers_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000732">
        <property type="entry name" value="PTS_enzyme_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47831">
        <property type="entry name" value="SSF47831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="SSF52009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01417">
        <property type="entry name" value="PTS_I_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 15 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 15 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="23" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="15 23" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PEP-utilisers_N" evidence="22">
        <location>
            <begin position="6"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers" evidence="20">
        <location>
            <begin position="155"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers_C" evidence="21">
        <location>
            <begin position="253"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="PEP binding" evidence="17">
        <location>
            <begin position="456"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="231"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="16">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="16">
        <location>
            <position position="504"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00016544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000732-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000732-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000732-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF05524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="EHS87335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="574" mass="62425" checksum="C06B43AAC4A19CC0" modified="2012-04-18" version="1">MSQVVKGIAASDGIGVAPAYLLVDPDLSYDKKKVDDTAAEYARVEKAFQDSISQLETIKENAKSRLGDEELAVFDAHIAILSDPEMLSQIKDDIESNQTSAEEAVDKVTTNFSDMLAAMIDNAYMQERAADVKDVAKRAMSNLMGKELPNIAGIDHPVVIVAHEITPSDTSQMDAKYVKGMVTDLGGRTSHAAIMSRTLRIPAIVGSQEITTTAKQGQTVIVDGLNGDAIVDPSEAEVKEYEDKAAAFEAERAEWAKMVDAPSKSKDGKDFEIAANIGTPDDVEDAMAQGADGVGLFRSEFLYMSSDHFPSEEEQFKAYKAAVVGMDGKPVVVRTMDIGGDKPLDYMPLPEELNPFLGYRAIRICLEQPDMFKTQLRALIRASEFGPIEIMFPMIATVAELRAAKQIYEEAKAEVQADHPGLGDNVKIGMMIEVPLAAVNADALAKEVDFFSIGTNDLIQYFFAADRGNDAVSYLYQPLNPALLSVIKHVIDAAHRHDAKAAMCGEMAGDQLALPLLMGMGLDEYSMSATSILRTRSMMSKLDTTECAKLVDDALNLDTAEEVEALVKERLNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIC0</accession>
    <name>H4GIC0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Beta-glucoside-specific phosphotransferase system (PTS), IIABC component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PS3_17211</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121804.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17211"/>
    <dbReference type="EnsemblBacteria" id="EHS87341">
        <property type="protein sequence ID" value="EHS87341"/>
        <property type="gene ID" value="PS3_17211"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.294"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00212">
        <property type="entry name" value="PTS_IIB_glc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036878">
        <property type="entry name" value="Glu_permease_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018113">
        <property type="entry name" value="PTrfase_EIIB_Cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001127">
        <property type="entry name" value="PTS_EIIA_1_perm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003352">
        <property type="entry name" value="PTS_EIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013013">
        <property type="entry name" value="PTS_EIIC_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001996">
        <property type="entry name" value="PTS_IIB_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00358">
        <property type="entry name" value="PTS_EIIA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00367">
        <property type="entry name" value="PTS_EIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02378">
        <property type="entry name" value="PTS_EIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55604">
        <property type="entry name" value="SSF55604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00830">
        <property type="entry name" value="PTBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51093">
        <property type="entry name" value="PTS_EIIA_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00371">
        <property type="entry name" value="PTS_EIIA_TYPE_1_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51098">
        <property type="entry name" value="PTS_EIIB_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01035">
        <property type="entry name" value="PTS_EIIB_TYPE_1_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51103">
        <property type="entry name" value="PTS_EIIC_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="280"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIB type-1" evidence="14">
        <location>
            <begin position="4"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIC type-1" evidence="15">
        <location>
            <begin position="111"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIA type-1" evidence="13">
        <location>
            <begin position="498"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate; for EIIB activity" evidence="11">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EHS87341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="623" mass="66541" checksum="56281F110D770B5D" modified="2012-04-18" version="1">MDYHKLAADIINNVGGQENVNSVGHCMTRLRFILKDVKKTDKDAIQNLDGVVGVVYAGGQYQVILGQNLLPVYEAIMNDFDVKAGQVINENVDEPKKEPWSWKNAGSKIIGFVSGAVMPMVPGLVAGGMLKVALLLVVTFIDANFAKSSSYLLLSVIADSPFYFMPIFVAYEAATKLGATPIYSMVAAAALLHNNFYSLVTQLAAHPNSVSASLFGINVPILSYSTSLLPALLISLVAYYTEKFLNKIVPGIFKAIFVGMGTIFVAGSLGFIILGPLGNYLGQVIASMFMFLNSTIGPFAVGLLAAALPWMVMAGMHQAITPFMPQLLTNPGYDALLRPAFTAHNMAEGGANIGVALRTKNKKFRSECWSLAVGAIVAGVTEPSIYGVNLRLKKPMYGVMAGGFAGGIVAGLLGARAYIMGYSNLLALAIFEKTALAMFAGCVTSIVVAAIVTYIVGFEDVPSEQQVSENEPVQKEYPDDAIVAVASGELENVEQVNDEVFSTRMMGDGVAFKLSGDFICSPANGEIVSLFPTGHAFGIQMNDGTELLVHIGLDTVKLNGEGFDVLAKQGEIVRAGQPIVKVDRKQIEDEGYDLTTILVITDAKNNDIKLKDQGSINVGDRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIE5</accession>
    <name>H4GIE5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Sugar transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_8979</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the sodium:galactoside symporter (TC 2.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8979"/>
    <dbReference type="EnsemblBacteria" id="EHS87366">
        <property type="protein sequence ID" value="EHS87366"/>
        <property type="gene ID" value="PS3_8979"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.341"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039672">
        <property type="entry name" value="MFS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001927">
        <property type="entry name" value="Na/Gal_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001127">
        <property type="entry name" value="PTS_EIIA_1_perm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11328">
        <property type="entry name" value="PTHR11328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00358">
        <property type="entry name" value="PTS_EIIA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00792">
        <property type="entry name" value="gph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00830">
        <property type="entry name" value="PTBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51093">
        <property type="entry name" value="PTS_EIIA_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00371">
        <property type="entry name" value="PTS_EIIA_TYPE_1_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIA type-1" evidence="9">
        <location>
            <begin position="431"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="565" mass="60593" checksum="CD214917A3378A6E" modified="2012-04-18" version="1">MGHFKPWVVGGGIISAVAIAILFTNMGGLTTRSPYLYLAIFAVIYIVMDIFYSFNDVAFWSMIPAISFDSEEREKTATFARIGSTIGGNLVGVIIMPVVLFFSIKSNGGTGDDRGWFMFALIVAGISAITAIGVGLFTHEKQNELRENKESTTIGQVLGVLVHNDQLMSIAVTYLFYTTGVTLLNSLELYYFQYIIGNSAAYSYLPLLNGVIGIFTVALYPALSKRFTRRNVFFGSVAIMLCAVVLFAFAGKSVVMATIAGVLFALPQPLNFLVVLMIITDSVEYGQLKLGHRDEGLVLSVRPLLDKFGGAVASGVVGLTATAAGMISGATADTITSHGSLVFKTMMFAVPAVMVIISVFIFAKKVKLSEQYHAEIVDELERTWHQNLAEDDTEAIATRDGDEDAAYGSNSTTQFLAPVDGKLLPLASVSDEAFASGSMGEGFAIDPSAGDVYAPFDGKVVATFPTNHAVALVSDDGIPVLIHVGVNTVEMQGEGFVSFVHNGQRVHADDKLLEFSSSAIKNAGKDDTVMVVFTKNEKSPDFDLTTIKDSGAVLHNEAVLEVTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIF1</accession>
    <name>H4GIF1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Galactokinase</fullName>
            <ecNumber evidence="11">2.7.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Galactose kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">galK</name>
        <name evidence="15" type="ORF">PS3_3770</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the transfer of the gamma-phosphate of ATP to D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>alpha-D-galactose + ATP = ADP + alpha-D-galactose 1-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13553"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28061"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="11">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the GHMP kinase family. GalK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.6" evidence="11"/>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121835.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3770"/>
    <dbReference type="EnsemblBacteria" id="EHS87372">
        <property type="protein sequence ID" value="EHS87372"/>
        <property type="gene ID" value="PS3_3770"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.347"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004335">
        <property type="term" value="F:galactokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00246">
        <property type="entry name" value="Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000705">
        <property type="entry name" value="Galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022963">
        <property type="entry name" value="Galactokinase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019741">
        <property type="entry name" value="Galactokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019539">
        <property type="entry name" value="GalKase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006206">
        <property type="entry name" value="Mevalonate/galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457:SF6">
        <property type="entry name" value="PTHR10457:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10509">
        <property type="entry name" value="GalKase_gal_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000530">
        <property type="entry name" value="Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00473">
        <property type="entry name" value="GALCTOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00131">
        <property type="entry name" value="gal_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00106">
        <property type="entry name" value="GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="6 11 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GalKase_gal_bdg" evidence="14">
        <location>
            <begin position="9"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP_kinases_N" evidence="12">
        <location>
            <begin position="116"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP_kinases_C" evidence="13">
        <location>
            <begin position="287"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="11">
        <location>
            <begin position="33"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="125"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="11">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF10509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EHS87372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="388" mass="43186" checksum="274DD023BFD5DBDD" modified="2012-04-18" version="1">MDKAELLAKYQDLFQVPGDDVFFSPGRINVIGEHTDYNGGHVFPCAISLGTYGVFGKRDDQEVHLYSGNMEGDEVIFDLNDDQPETDDDRKWANYFKGMLVYLKQGGFTIDQGFNLYVYGNLPYGAGLSSSASIELLMGNVLKDEYKLEIEQLDLVKLGQKTENDFVGLNSGIMDQFAVGMSKKDNAIFLDCNTLEYEYLPLNLGDYEIVIMSTNKKHSLAGSAYNDRVAECGEAVKRLQQKLDIQTLGELDEHTFDEYSYLINDELLIKRARHAVLENDRTLRATKAMQDGDLEKLGRLINASHVSLHYDYEVSGTELDTLAEAAWAQPGVLGARMIGGGFAGSAIAIVKKDQAENLKQAVGKIYRDKVGYDASFYDAEIVDGPHKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIF8</accession>
    <name>H4GIF8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyltransferase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_13431</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000015">
        <property type="protein sequence ID" value="EHS87307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121842.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000015.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13431"/>
    <dbReference type="EnsemblBacteria" id="EHS87307">
        <property type="protein sequence ID" value="EHS87307"/>
        <property type="gene ID" value="PS3_13431"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.355"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="378"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl_transf_3" evidence="2">
        <location>
            <begin position="13"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="614" mass="70230" checksum="B1A80A998E9B5AEE" modified="2012-04-18" version="1">MGNRQTGRRHITGVDGLRAIAVIGVIIYHLMPSVAQGGFLGVPIFLLISGYFVTCQLVVEYDHTNRIDFFHFYHKRFERLYPTLVAMLIVTTAYITLFQRSLLVNLRSTIWTNLLWVYNWWEIGHGQSYFDRFAGESPFTHLWTLGVEVQFYVLWPMLLVILLMMFYQRRQRVRWVVLLLAILSALEMAVLYHPTSINRVYYGTDTRAFSLMLGAFLGLIWPINHLRPNIDRAWSTALDFLGILTLLGTIFGFFTLSGQDAATYRGAMFGYSFLGTILLATIVHPGAHMNRLLTNHLFTWIGKRSYGIYVYQFPVMIFYENVVKVGNHPLIHGIIQLAIIMGISELSYRYFERPLANYDWHNLGQTLRTLFNRTQWRRWLVVGPALILTGIAIYGLCLPEPKPTATAVQKRITQNAKMIAKRNQEIAAGKTPQVNVNSNSIKQRYDLTTKQVKKLQRLKITAVGDSVMLDASQSIQQLMPLAYVDGQVGRQGNDTPTVLKTLASQGRLANIVVLNLGTNGPIKDQAVDEMLSIIGSKRQVYWVTAHVPTRSWQDSVNDQIKKAAKTHQNVHLVDWYQLSKDHNDWFASDQVHMQEKGNVQFTRLIATTILKYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIF9</accession>
    <name>H4GIF9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_4773</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000015">
        <property type="protein sequence ID" value="EHS87308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121843.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000015.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4773"/>
    <dbReference type="EnsemblBacteria" id="EHS87308">
        <property type="protein sequence ID" value="EHS87308"/>
        <property type="gene ID" value="PS3_4773"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.356"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="102" mass="12040" checksum="A3D7CDF43D944F51" modified="2012-04-18" version="1">MAEIGVLDLIEQITRLDGSKYYEIGNMVHNGRAELAAERGFIKEVRILKLNIPHSQTVIKYEQFVNEHYQMQDESMDHWEEWPRNDEADEWVQTIVQDNHVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIJ6</accession>
    <name>H4GIJ6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphatidylglycerol lysyltransferase</fullName>
            <ecNumber evidence="6">2.3.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Lysylphosphatidylglycerol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mprF</name>
        <name evidence="7" type="ORF">PS3_4099</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the transfer of a lysyl group from L-lysyl-tRNA(Lys) to membrane-bound phosphatidylglycerol (PG), which produces lysylphosphatidylglycerol (LPG), a major component of the bacterial membrane with a positive net charge. LPG synthesis contributes to bacterial virulence as it is involved in the resistance mechanism against cationic antimicrobial peptides (CAMP) produces by the host's immune system (defensins, cathelicidins) and by the competing microorganisms.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + L-lysyl-tRNA(Lys) = 1,2-diacyl-sn-glycero-3-phospho-1'-(3'-O-L-lysyl)-sn-glycerol + tRNA(Lys)</text>
            <dbReference type="Rhea" id="RHEA:10668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
            <dbReference type="ChEBI" id="CHEBI:75792"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="EC" id="2.3.2.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the LPG synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.3" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000020">
        <property type="protein sequence ID" value="EHS87275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121880.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000020.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4099"/>
    <dbReference type="EnsemblBacteria" id="EHS87275">
        <property type="protein sequence ID" value="EHS87275"/>
        <property type="gene ID" value="PS3_4099"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.404"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050071">
        <property type="term" value="F:lysyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00374">
        <property type="entry name" value="TIGR00374"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="339" mass="38329" checksum="E24599C03A21C488" modified="2012-04-18" version="1">MNRKNWLALILMLVVGVGIFAFSLRNTDLHQLLIDLLALNWGWFAIAIVCIGLYLGLEGVVVKLFMKDSYPSFSWKDAMRMPLIEQLFNGITPFSSGGQPAQIIAMLQSGVDGGRASSVLLMKFVVFQAMIVINFIVSLIMGFHLISTKLQGLTLFVVIGFIIHFAVIVGLLMIMYWYQMTKRLTNWVIKPLRLFMKPERYASIAVTLNEKIDTFYKESVKMKKQYRKLAKVSVVTLLQLFFYYAIPYFILRALGVSGLNFWMILSLHVIIFMVTSVFPIPGGTGGAEYGFVVLFAGYVQSNSKLVLAMLLWRILTYYVGLFAGMFALVIKPDKVSEID</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIL5</accession>
    <name>H4GIL5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Malonyl CoA-acyl carrier protein transacylase</fullName>
            <ecNumber evidence="4">2.3.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_6366</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>holo-[ACP] + malonyl-CoA = CoA + malonyl-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the fabD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.39" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121899.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6366"/>
    <dbReference type="EnsemblBacteria" id="EHS87210">
        <property type="protein sequence ID" value="EHS87210"/>
        <property type="gene ID" value="PS3_6366"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.426"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024925">
        <property type="entry name" value="Malonyl_CoA-ACP_transAc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000446">
        <property type="entry name" value="Mct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="SSF55048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_AT" evidence="6">
        <location>
            <begin position="6"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000446-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="316" mass="34027" checksum="213E3F3EE8469B82" modified="2012-04-18" version="1">MRLGILFSGQGSQKPGMGIDLLADPPFLKTVKTASEASGLDIETILRGENGELDQTINVQPALVTFSYGIYRMLQRDVPELPIAAMAGLSLGEYAALIASQAIDLSSGISLLADRAKYMQADADAVTSTMAAIVKPNLPEVLAICEASQKNGQPVYVANYNSPSQVVLGGTLPNLQVAVDQITANGYAKKAVILKVNGAFHTPLFNGARAKMHDRLQSVDFKEPVVPMISNTTVQPLHADEIKAVMECQLAVPTHFGEDLQYMIDHQGVDATLEIGPGKTLSQFAKQVDRGLTRYHIETLADYQEFVKEIQNGTQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIM8</accession>
    <name>H4GIM8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thymidylate kinase</fullName>
            <ecNumber evidence="3">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tmk</name>
        <name evidence="5" type="ORF">PS3_11358</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>ATP + dTMP = ADP + dTDP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the thymidylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121912.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11358"/>
    <dbReference type="EnsemblBacteria" id="EHS87223">
        <property type="protein sequence ID" value="EHS87223"/>
        <property type="gene ID" value="PS3_11358"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.442"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:thymidylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate_kin" evidence="4">
        <location>
            <begin position="8"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="10"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="217" mass="24357" checksum="1BE45FB233B50FBB" modified="2012-04-18" version="1">MTGKFISFEGPDGAGKTSVIKAIVDRLADLNLANQVVLTREPGGTPIGEAIRDLLLSAKSVDMDGWTEAFLFAASRRQHVVEKIKPALKADQIVLCDRFVDSSVSYQGQGRQLGFDQIWELNQAAIGDCLPDLTIVLDLPIEEGLQRITQHRTDQINRLDEETLTFHQRVRQGFLKQAQRDPQRIVVVDASQELTAVVKDVWHQMAERWPELKNEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIN0</accession>
    <name>H4GIN0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoid-associated protein PS3_8635</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_8635</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to DNA and alters its conformation. May be involved in regulation of gene expression, nucleoid organization and DNA protection.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the YbaB/EbfC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121914.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8635"/>
    <dbReference type="EnsemblBacteria" id="EHS87225">
        <property type="protein sequence ID" value="EHS87225"/>
        <property type="gene ID" value="PS3_8635"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.444"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043590">
        <property type="term" value="C:bacterial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1310.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00274">
        <property type="entry name" value="DNA_YbaB_EbfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036894">
        <property type="entry name" value="YbaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004401">
        <property type="entry name" value="YbaB/EbfC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33449">
        <property type="entry name" value="PTHR33449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02575">
        <property type="entry name" value="YbaB_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004555">
        <property type="entry name" value="UCP004555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82607">
        <property type="entry name" value="SSF82607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00103">
        <property type="entry name" value="DNA_YbaB_EbfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="102" mass="11462" checksum="BFEE13255DAAD1C9" modified="2012-04-18" version="1">MMRGMNMQQMMKQAQKMQKQMMAEQQELAEQTFVGKAPDEMVVATFTGDKKLTDLQIKPAVVDPDDVDMLQDMVLTAINDGLEKVDQATQSKMGKYTRGLGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIP0</accession>
    <name>H4GIP0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Neopullulanase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_10395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10395"/>
    <dbReference type="EnsemblBacteria" id="EHS87235">
        <property type="protein sequence ID" value="EHS87235"/>
        <property type="gene ID" value="PS3_10395"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.458"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aamy" evidence="1">
        <location>
            <begin position="38"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="505" mass="58428" checksum="30D84005201A41C2" modified="2012-04-18" version="1">MGQIALTDDYDQLQKFQLTCYQFENPTPDWYRHGIAYQIFPDRFFNGHPHKEITGRKPNSFLYATEQDQPYYIKNSDGEIVRWDFYGGNIAGIQAKIPYLKSLGVTILYLNPIFAATSNHRYDTNDFFTIDPMLGSEAEFKELVTSLHQNGIHLILDGVFNHVGEDSIYFNAHQLYGPNTGASQNQHSPYYSWFKFTHYPDKYASWWGVSNLPEVNKADPSYQDFIYGNQGVLHKWDQLGVDGWRLDVADELPMDFLRQLRHRERIEGNQVLIGEVWEDASNKYVGNELRTYPNGDNLSGTMNYPVRSFITNYLCQPDATKTMTDYLALIENYPREFMENCLNNIGTHDTIRIKTALNNDEQLVQIAFAMLFMLPGVPCLYYGDEAGLVGAKDPDNRRFFPWDQINQTIQATVKCWIKRRQLDVLLVTGDIGLAYDKQGHLIIIRYQDHQADLLLINPLECSWTVDPHQLTYLHLPATICDQLGHFFNGKTSRRKTKSIYKTIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIP1</accession>
    <name>H4GIP1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L7/L12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rplL</name>
        <name evidence="7" type="ORF">PS3_10557</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Is thus essential for accurate translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Part of the ribosomal stalk of the 50S ribosomal subunit. Forms a multimeric L10(L12)X complex, where L10 forms an elongated spine to which 2 to 4 L12 dimers bind in a sequential fashion. Binds GTP-bound translation factors.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121925.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10557"/>
    <dbReference type="EnsemblBacteria" id="EHS87236">
        <property type="protein sequence ID" value="EHS87236"/>
        <property type="gene ID" value="PS3_10557"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.459"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00387">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00368">
        <property type="entry name" value="Ribosomal_L7_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000206">
        <property type="entry name" value="Ribosomal_L7/12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_L7/12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013823">
        <property type="entry name" value="Ribosomal_L7/L12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008932">
        <property type="entry name" value="Ribosomal_L7/L12_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036235">
        <property type="entry name" value="Ribosomal_L7/L12_oligo_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987">
        <property type="entry name" value="PTHR45987"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00542">
        <property type="entry name" value="Ribosomal_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16320">
        <property type="entry name" value="Ribosomal_L12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48300">
        <property type="entry name" value="SSF48300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="SSF54736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00855">
        <property type="entry name" value="L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal_L12_N" evidence="6">
        <location>
            <begin position="5"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L12" evidence="5">
        <location>
            <begin position="55"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="121" mass="12310" checksum="BCFE754678289D15" modified="2012-04-18" version="1">MAFDKDAIIASLKEASISDLNDLVKAIEEEFDVSAAAPVAVAGAAGGEAAAKDSFTVELTEAGAAKVKVIKVVKDITGLGLKDAKAIVDGAPAAVKEDVKEDEANDIKAKLEEVGATVTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIQ5</accession>
    <name>H4GIQ5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Zinc/iron ABC transporter permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_4604</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ABC-3 integral membrane protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121939.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4604"/>
    <dbReference type="EnsemblBacteria" id="EHS87250">
        <property type="protein sequence ID" value="EHS87250"/>
        <property type="gene ID" value="PS3_4604"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.475"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001626">
        <property type="entry name" value="ABC_TroCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30477">
        <property type="entry name" value="PTHR30477"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00950">
        <property type="entry name" value="ABC-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="264" mass="28564" checksum="C3D9181734531003" modified="2012-04-18" version="1">MFALSFMRHAFVASTLIAILSGVIGVFVVARQMSFMTHTLSEIGFAGGSFAVFAGWSSLNGMILFTVLSSVLVGQLSLKESRREAVISAVSALFIGLGILFLSLSSQTASSATSILFGSVVGISITEVWQLLWLTILILAAVFLMYRRLKFTSFDRDGAKVSAINETVISILFLLILAVSVSVTAQIVGSLLIFILLTLPAASAKYYTTGVAKMIGLAILFSLLGTWLGLYLGYITNWPVSFFIAVIEVLIYLSALIYRHYQVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIR6</accession>
    <name>H4GIR6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-hydroxy-3-methylglutaryl coenzyme A reductase</fullName>
            <shortName evidence="3">HMG-CoA reductase</shortName>
            <ecNumber evidence="3">1.1.1.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_7929</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(R)-mevalonate + CoA + 2 NAD(+) = (3S)-hydroxy-3-methylglutaryl-CoA + 2 H(+) + 2 NADH</text>
            <dbReference type="Rhea" id="RHEA:14833"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.88"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Metabolic intermediate metabolism; (R)-mevalonate degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from (R)-mevalonate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.88" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121950.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7929"/>
    <dbReference type="EnsemblBacteria" id="EHS87261">
        <property type="protein sequence ID" value="EHS87261"/>
        <property type="gene ID" value="PS3_7929"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.486"/>
    <dbReference type="UniPathway" id="UPA00257">
        <property type="reaction ID" value="UER00367"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140643">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00644">
        <property type="entry name" value="HMG-CoA_reductase_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004553">
        <property type="entry name" value="HMG_CoA_Rdtase_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="PTHR10572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="SSF55035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="SSF56542"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00532">
        <property type="entry name" value="HMG_CoA_R_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="418" mass="45478" checksum="0A8F2F8FD68B471E" modified="2012-04-18" version="1">MTKEWYQGFYKRSIAQRCQLLQEHLGLTAEQCQLLFSQSDSLSSQLIENYLTDYHLPEGLAVGLVVNGQNYALPMVVEEPSVVAAASNGAQRIAKNGGFMAYPGGRKVIGQVVLEQVENFDQCRQWIFDHQATILQVANQAHPSLQKRGAGAIELRVRRIEPFISVDLVIDVSQAMGANSVNTMAEAVSQWFQEQGYQVLTAILSNYAVDSLQTVTGKVHVRDLGTIDQDGRQVAEKIVQLSQLAQVDPYRAATHNKGIMNGIDAAVIASGNDWRAIESGAHAYASRDGHYRGLSQWHLDGDYLVGQLTLPMPVGVVGGSIKLLPQSQINYQISHIQTAEELAGVIASVGLAQNLAALRALGSTGIQAGHMKLQYRSLAISVGAKEEEIAKLVQALIASPRVDTQVATKLLKELRGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIS4</accession>
    <name>H4GIS4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RpoE protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_7289</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Might take part in the signal recognition particle (SRP) pathway. This is inferred from the conservation of its genetic proximity to ftsY/ffh. May be a regulatory protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0122 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000022">
        <property type="protein sequence ID" value="EHS87190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7289"/>
    <dbReference type="EnsemblBacteria" id="EHS87190">
        <property type="protein sequence ID" value="EHS87190"/>
        <property type="gene ID" value="PS3_7289"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.501"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007394">
        <property type="entry name" value="UPF0122"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04297">
        <property type="entry name" value="UPF0122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="199" mass="23366" checksum="A2D8D07270E35FAF" modified="2012-04-18" version="1">MTLGQENIKILRNIIMINRTMRDGFLHLTLHHNDRLIKISLHKLHIHPDHRQYDDLYQEGCLAYAKAYVSFDGDPETYADLDWFQAYAYRRIYWRLLDLLKHSWNYVQAQEISADQSQATNLALQSQADPSASQALQKADLEHALRYIRAHSTPLQNQYLSLVLDSDLTNAEIARILGISRQSVYELRRRVQEVVKRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIT9</accession>
    <name>H4GIT9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">Adenylate kinase</fullName>
            <shortName evidence="6">AK</shortName>
            <ecNumber evidence="6 8">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">ATP:AMP phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Adenylate monophosphate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">adk</name>
        <name evidence="10" type="ORF">PS3_14601</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from ADP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis. Some bacteria have evolved a zinc-coordinating structure that stabilizes the LID domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the adenylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="6 8"/>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14601"/>
    <dbReference type="EnsemblBacteria" id="EHS87169">
        <property type="protein sequence ID" value="EHS87169"/>
        <property type="gene ID" value="PS3_14601"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.519"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00649"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006259">
        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007862">
        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="PTHR23359"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05191">
        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01351">
        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="4 6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ADK_lid" evidence="9">
        <location>
            <begin position="130"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMP" evidence="6">
        <location>
            <begin position="33"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="60"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="88"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="139"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="221" mass="24692" checksum="8E7DE8F8739C941E" modified="2012-04-18" version="1">MSTMNLVLMGLPGAGKGTQAQLIVQEFDVPHISTGDIFRAAIKNETPMGIQAKQFIDKGELVPDEVTNGIVKERLAEADTAKGFMLDGFPRNLVQAESLNQMLADSGRKLDAVINIHVNPDILVERLSGRFICRNCGTTYHRLYNMPKVEGTCDVCGQHDFYQRDDDKPETVKNRLDVNIKLNTPLIDFYEQQGVLYNIDGEQDIDKVYSDVKKVLTSLTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIW0</accession>
    <name>H4GIW0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetoin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_20025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121993.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20025"/>
    <dbReference type="EnsemblBacteria" id="EHS87132">
        <property type="protein sequence ID" value="EHS87132"/>
        <property type="gene ID" value="PS3_20025"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.544"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="264" mass="28554" checksum="A39984257DD62198" modified="2012-04-18" version="1">MTEAKRVAVITGGERGIGRGITDQLLKDGYYVVIANRDEAAGQQAVDELNAQGYTGKVVAVAGNAGQKASHERFVQAAVDNFGRLDTYINNAGIAQIKLLQDETEADMEEIYRTNVFSVLYGIQAATAQFRKQDDGKQIRKIINASSIAGHIAFDMLGAYSSTKFAIRGLTQAAAKELGRDHITVNAYCPGIVGTDMWDYIDEQMVKAWGGNKGDYLKKYSGSITLGRVETPADVANYVSYLASPVSDYMTGQAVQIDGGIQFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIX5</accession>
    <name>H4GIX5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thiamine-phosphate synthase</fullName>
            <shortName evidence="8">TP synthase</shortName>
            <shortName evidence="8">TPS</shortName>
            <ecNumber evidence="8">2.5.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Thiamine-phosphate pyrophosphorylase</fullName>
            <shortName evidence="8">TMP pyrophosphorylase</shortName>
            <shortName evidence="8">TMP-PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">thiE</name>
        <name evidence="12" type="ORF">PS3_6732</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:47848"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62890"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 8 9">
            <text>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:47844"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8 9">
            <text>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 4-methyl-5-(2-phosphooxyethyl)-thiazole + H(+) = diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:22328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 8 10">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the thiamine-phosphate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.3" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122008.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6732"/>
    <dbReference type="EnsemblBacteria" id="EHS87147">
        <property type="protein sequence ID" value="EHS87147"/>
        <property type="gene ID" value="PS3_6732"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.570"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00141"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004789">
        <property type="term" value="F:thiamine-phosphate diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00564">
        <property type="entry name" value="TMP_TenI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00097">
        <property type="entry name" value="TMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036206">
        <property type="entry name" value="ThiamineP_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022998">
        <property type="entry name" value="ThiamineP_synth_TenI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034291">
        <property type="entry name" value="TMP_synthase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02581">
        <property type="entry name" value="TMP-TENI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51391">
        <property type="entry name" value="SSF51391"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00693">
        <property type="entry name" value="thiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8 9" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="5 8 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TMP-TENI" evidence="11">
        <location>
            <begin position="10"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="HMP-PP binding" evidence="8">
        <location>
            <begin position="42"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="THZ-P binding" evidence="8">
        <location>
            <begin position="140"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="217" mass="23229" checksum="CFFD0EDEAB4ADEFE" modified="2012-04-18" version="1">MQFKPEMLHCYLIGGSQDVDHDPTKFLLKLTTACQAGITAFQFREKSDLVEYDVYALGFQVRTICSQFQVPLFVDDNLELAQKINADGIHVGQKDQPIEEVIQAAGDDLMIGYSCNTVAQVEHANQVQVDYIGSGPIYPTKSKADADPALGVKELGHLVQISQHPVVAIGGITPAKVPEVLTSGCAGISAIAGIFDNLAAGMPAGPDNPVSQILNCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIY8</accession>
    <name>H4GIY8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_11952</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11952"/>
    <dbReference type="EnsemblBacteria" id="EHS87100">
        <property type="protein sequence ID" value="EHS87100"/>
        <property type="gene ID" value="PS3_11952"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.590"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021462">
        <property type="entry name" value="DUF3114"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11311">
        <property type="entry name" value="DUF3114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="394" mass="45736" checksum="9ECB3B645D7A4C69" modified="2012-04-18" version="1">MREQLKAEVKDLRLDGWDRAAIKAYLAYLGRYTLKDAQSDQVTPQQKIKSYWRSVHNVGSKIYTSLFNASRLKPYAKLNLLLNRQLGGYIDQNGFLQLPANRKYHLKAQISPTSSFFPAMRDTVVAAYKNNSKGLKADSLGQELHLFRSYLDRSYLNYIRQYDGLKHDLPAEATDYQRLQQFMKDHHLEADYSTGANYHNRYQGTFTYPENMKVQLMKDSTAKTKNDGRMIEFIVNIQNGSFVSEWNAYDQHLNADGTVNSDPSIYSNQEQEMIANTESFNYGVPSGGQGNHVPSTYYKTHQNLDVSHPKDPEIRQQATAKNKFKSEADYDKGGDYVDIVKNGEKDIKSWDDYIKHKDNRSISNEYNKYVAWTKKYHQYPNYQPGFSYYLEHQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ04</accession>
    <name>H4GJ04_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA-entry nuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_15344</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122037.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15344"/>
    <dbReference type="EnsemblBacteria" id="EHS87116">
        <property type="protein sequence ID" value="EHS87116"/>
        <property type="gene ID" value="PS3_15344"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.615"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01223">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003591867" evidence="1">
        <location>
            <begin position="28"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease_NS" evidence="2">
        <location>
            <begin position="104"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="280" mass="30637" checksum="564008BAE51A6C42" modified="2012-04-18" version="1">MSRKKRSKGLFKWVVTLFALLGIFVGAQSSLSPNLEQVWSNLITRVSGTVDGEASSLITPSEDQARSVLTNQVEQQLGDQITWNDHGAFIINHNQTNLNASVASAPYAVNRYDSAGRPWQGDAWLNRTTRQYRNRQQTGNGATNWRPAGFLQATNLTGGPSHAYDRGHLLGYALVGGLSNFDASESNPANIATQTAWANEARAKTSTGQNYYEGLVRQALDRNKQVRYRVTDLYDGDNIVPSGAHIEAKSSDGSLEFNVFVPNVQNNITVNYATGAVTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ23</accession>
    <name>H4GJ23_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MFS family major facilitator transporter, multidrug:cation symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_9993</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the major facilitator superfamily. EmrB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000027">
        <property type="protein sequence ID" value="EHS87099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122056.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000027.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87099">
        <property type="protein sequence ID" value="EHS87099"/>
        <property type="gene ID" value="PS3_9993"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.653"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="427"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="9">
        <location>
            <begin position="14"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="447" mass="48233" checksum="CC739D667785A54A" modified="2012-04-18" version="1">MKTQSNFFNSNPISIAILFAGTLLGLLTQNMMATILTPIINDFQISASTAGWLTTIYSLIASILVPVSAFFTKRYSCRQLFSFSMLTFTIGSFIGIIATNFEVLLFSRILQGIGTGILMSLLQIAIYCSCSIEQRNKLMGIIGVSMGFAPAFGPVLSGFMSDAWSWKSVFVLLTIMGAILLFLSLLLLKNLTKQEENCFLDKLSLLLSSVGFSGFTLGISFISQFGLKSAITLSCLAAGLLIIFLFIHRQFRISQPLLQLETLKNKGMLTGTLFTFLLFTAFNGVSVVLPLYMQKFIGISASVSGIVTFPGAVLVAVASLISGFLADKFGFHRLTVAASIMLCVGTGLLLTLNTGSSLVMIAAVQCIRNFSIGLLIMPVTTWALNQLNTNLVSDGTAIINTVRQISGAIGTSCMLMFLQLFGVSTAFFFSLTLSILSLVIAVQKIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ37</accession>
    <name>H4GJ37_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Beta-phosphoglucomutase/glucose-1-phosphate phosphodismutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_12312</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="3">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122070.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12312"/>
    <dbReference type="EnsemblBacteria" id="EHS87062">
        <property type="protein sequence ID" value="EHS87062"/>
        <property type="gene ID" value="PS3_12312"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.676"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008801">
        <property type="term" value="F:beta-phosphoglucomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02598">
        <property type="entry name" value="HAD_BPGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010976">
        <property type="entry name" value="B-phosphoglucomutase_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010972">
        <property type="entry name" value="Beta-phosphoglucomutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01990">
        <property type="entry name" value="bPGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02009">
        <property type="entry name" value="PGMB-YQAB-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="47"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="119"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity and assists the phosphoryl transfer reaction to Asp8 by balancing charge and orienting the reacting groups" evidence="4">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity and assists the phosphoryl transfer reaction to Asp8 by balancing charge and orienting the reacting groups" evidence="4">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR610972-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR610972-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR610972-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR610972-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="220" mass="23873" checksum="648BDF4CD81F5B1B" modified="2012-04-18" version="1">MKFEQIKGFAFDLDGVIADTARFHGQAWHQLADQVGTPWTQELADGLKGVGRMDSLEMILKAGGHENDYSLAEKEALAKAKNDNYLHLVETLTPEDILPGMKDFLDELKAADYQLILASASKNAPKVLKYLKLSDYFVDIVDPAQLVQSKPAPDIYIQAAKQLGLSPDEVAGVEDAQAGVDAINAADELSIGIGTNLTNADVRFDQTSDVSLEALKAKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ59</accession>
    <name>H4GJ59_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_14223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122092.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14223"/>
    <dbReference type="EnsemblBacteria" id="EHS87084">
        <property type="protein sequence ID" value="EHS87084"/>
        <property type="gene ID" value="PS3_14223"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.706"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="257" mass="28037" checksum="4BD34AABCA6A3A97" modified="2012-04-18" version="1">MESFNSTSFRLDGRVALVTGGAKGLGKYYSIALAMYGAKIFVVSRSRDGWDELRSKVSELGSEVNFLQEDISQTDCADQIVEAVIKQYGHLDILVNNAGIQIRNDVLAFKDEDWDKVINLNLNALYHLSHAAAKVMVKQSSGKIINIGSMQSYRAGKYIFPYTASKHGVLGLTKAYADALAPYHIQVNGLAPGYIKTDMTKALQDDPVRGPEIQAHIPSGDWGVPENLMGPMIFLASSASDYVTGVMLPVDGGYLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ60</accession>
    <name>H4GJ60_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_19790</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122093.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19790"/>
    <dbReference type="EnsemblBacteria" id="EHS87085">
        <property type="protein sequence ID" value="EHS87085"/>
        <property type="gene ID" value="PS3_19790"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.707"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="2">
        <location>
            <begin position="93"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="222" mass="25215" checksum="E893E1C53AD76085" modified="2012-04-18" version="1">MLIQQDHGRKLRFYVSGILFIIIALMVWKSTLLPSTVDAALQTLFTSNHASGFMTFIMKFISFIGSPKMTILWTLVIAFLLWGFKFKVPAVWSVVTLFGADVLGWIVKHIIQRARPSQHLASDDGYSFPSGHVLGTFIVAAIILLILVPLIQNRLKRNLTIALTLIFVILLAISRVYLYAHYPTDTIGAMLLAYTWVQVMEMAYVKYAPTLQHWNFTHNSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ63</accession>
    <name>H4GJ63_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cation efflux system protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_9408</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122096.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9408"/>
    <dbReference type="EnsemblBacteria" id="EHS87088">
        <property type="protein sequence ID" value="EHS87088"/>
        <property type="gene ID" value="PS3_9408"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.710"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="SSF160240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="297" mass="33153" checksum="DFA67228B2005310" modified="2012-04-18" version="1">MHQHQVSGKRFGGVTLLNAIITVVEILGGIFSGSLSLLSDAMHNLSDTVSIAIGYGAQIISQRPENSKRTYGYQRAQILAALFNAALLVLIAVVLIIEAIQRFQHPEKIDGPLMLVVAVIGLIANFVSMFLLNAGKDTSLNIKATYLHLLSDTLSSVAVIIGALVLTFTDAEWVDPLLTIIVALYIAYEAWPIIKTTFNILMQTSPAVNYDEIERDLLAIDGICHVHHLHAWMIDEHHNSMSLHINCNDIRLSEAEQLYQQVDQLLKDKYQIDHVTIQAECYRGRHERLFNPDSDQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ69</accession>
    <name>H4GJ69_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA-binding protein Dps</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_11201</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Dps family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122102.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11201"/>
    <dbReference type="EnsemblBacteria" id="EHS87024">
        <property type="protein sequence ID" value="EHS87024"/>
        <property type="gene ID" value="PS3_11201"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.716"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016722">
        <property type="term" value="F:oxidoreductase activity, acting on metal ions"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01043">
        <property type="entry name" value="DPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002177">
        <property type="entry name" value="DPS_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023188">
        <property type="entry name" value="DPS_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42932">
        <property type="entry name" value="PTHR42932"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005900">
        <property type="entry name" value="Dps"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01346">
        <property type="entry name" value="HELNAPAPROT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00818">
        <property type="entry name" value="DPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin" evidence="3">
        <location>
            <begin position="8"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="155" mass="18135" checksum="EF4EE5430049D4F8" modified="2012-04-18" version="1">MKYPQTKEVLNQLVADLSQLSMVVHQTHWYMRGTNFLKLHPLMDEWQEDLDAQLDVISERLIALDGSPYSTLTEMADHTKIPDEPGTWDKSTPERFEIFSKDYRYLADLYQKGIEVSDDEKDFSTQDIFIGFKTETEKRLWMIQAELGKAPERDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ90</accession>
    <name>H4GJ90_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Acetyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_9963</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122123.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9963"/>
    <dbReference type="EnsemblBacteria" id="EHS87045">
        <property type="protein sequence ID" value="EHS87045"/>
        <property type="gene ID" value="PS3_9963"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.753"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039369">
        <property type="entry name" value="LacA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024688">
        <property type="entry name" value="Mac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43017">
        <property type="entry name" value="PTHR43017"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12464">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01266">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Mac" evidence="5">
        <location>
            <begin position="7"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="188" mass="20605" checksum="CB8FDC49CD597009" modified="2012-04-18" version="1">MKLEDQYQRILSGQMYDDLTPELVTARERTVLLTNQYNASFGQPAPEREAILKKLLGHIGQGVHFEPTFRYEFGNHIQIGNNFYANFDCVMLDGGGIEIGDNVLFGPKVGIYTSNHAFDAWERTHGACYAKPVKIGNNVWVGAGVHINQGVTIGDNTIIGSGSVITKDIPANVVAAGVPCRVIREITE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ98</accession>
    <name>H4GJ98_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Prophage Lp2 protein 49</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_22283</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000031">
        <property type="protein sequence ID" value="EHS87010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122131.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000031.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_22283"/>
    <dbReference type="EnsemblBacteria" id="EHS87010">
        <property type="protein sequence ID" value="EHS87010"/>
        <property type="gene ID" value="PS3_22283"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.774"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006520">
        <property type="entry name" value="Dit_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008841">
        <property type="entry name" value="Siphovirus-type_tail"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05709">
        <property type="entry name" value="Sipho_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01633">
        <property type="entry name" value="phi3626_gp14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="306" mass="34248" checksum="0236396A2ABB5173" modified="2012-04-18" version="1">MIKVFSNRTDKPKQYGFVSIDEAPDKNTVGYHPVEIAVSDNATDWVSIYDHPDLEGVHVVDTPAISASSQSNTSQKLGINDGKSLVSSSFDQREIKMTLRFDSRDGSDSRLAYDALQRFLVTREPYWICFADFPERMYYVKVTGVDNTTFNDWGFLVEYTLTDQLGLSRSIGTTGDQNNYLLGFGNNELLTAPPQYSFRGPAFTLYNPSDIVIDPERRGHPFKMILEGSASGDMTIRNATTATSIARKGAWSGKWVLDGVNPFLNDKNDGINTDHGIITLQIGDNKFEVSGFDGELKFDYPVWWLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJB2</accession>
    <name>H4GJB2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Polyketide transporter ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_4234</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000032">
        <property type="protein sequence ID" value="EHS87002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122145.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000032.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87002">
        <property type="protein sequence ID" value="EHS87002"/>
        <property type="gene ID" value="PS3_4234"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.805"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="290" mass="32904" checksum="A0EF2BE73B56DBC6" modified="2012-04-18" version="1">MAKNMIVIDNLTKDYGSGRGVFNMDLQIKQGEVFGLVGVNGAGKTTTFRMLMGFIKANNGNAQIDGLDIWKNASTIKNYVGYVPGEIAFPDTRSGQTFLKLQLDLLHQKAHENQKVNDLIQKLKIDTTADIKRMSKGMKQKVALVCAFMTDAKVLLLDEPTTGLDPVMRDVFIDLVKDAKSQQKTILMSSHMFNELEPTCDRIAFLKKGKIFDLLDQKKMDNLRLHQEFKIELEDEKSYQRLLKSNFKVSGYDTNKKIVKLTIHEKEDRNLIQELGMVNVKEVSIKDGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJC3</accession>
    <name>H4GJC3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Allophanate hydrolase subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_21478</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000033">
        <property type="protein sequence ID" value="EHS86992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122156.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000033.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21478"/>
    <dbReference type="EnsemblBacteria" id="EHS86992">
        <property type="protein sequence ID" value="EHS86992"/>
        <property type="gene ID" value="PS3_21478"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.824"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003778">
        <property type="entry name" value="CT_A_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02626">
        <property type="entry name" value="CT_A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00797">
        <property type="entry name" value="AHS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00724">
        <property type="entry name" value="urea_amlyse_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AHS2" evidence="4">
        <location>
            <begin position="25"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="338" mass="37200" checksum="DC9772C902ED72A0" modified="2012-04-18" version="1">MPEMRIIHPGPFTTVQDLGRTQSQVAGFPASGALDRQAAEIGNALVNNRDEAAVLEFFLQGPTIYFTHATYIALTGAQLVATLDGQTIPMYRCQRVQAGQILTIGQLHAGQVAYLAVSGGVRVPRVLGSRSTTTRIQLGGLNGRILESGDRLPVMQCELMPAYYHRTTEALPLPDLAEPLRLRILKGPQWSWFDQATQAQFINSQYEVTKDADRMGYRLSGPQLPTPDKSMLSAATVFGGIQVPNGGQPIVLLADRQTTGGYPLIAVLATVDIRLLAQSRPGQAIQFELIDLEEAQALIRERSHSQSQLRQKFHKAYYQAPFGISRVAAYRIQQLFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJC6</accession>
    <name>H4GJC6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Immunity protein PlnI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_7158</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0177 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000033">
        <property type="protein sequence ID" value="EHS86995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122159.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000033.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7158"/>
    <dbReference type="EnsemblBacteria" id="EHS86995">
        <property type="protein sequence ID" value="EHS86995"/>
        <property type="gene ID" value="PS3_7158"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.828"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="244"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="408" mass="46823" checksum="79E4D7DA2F51C058" modified="2012-04-18" version="1">MSGNDYLKIWHYAQLGITLFFIVVIAIWETVHTIQLSAFVITVGIFFVLFLMGDFVKRKPQWLTIIMDWLKAIVQPIALILVWAIVTRQIIVHTGLAVRGVISLSFIIYIIMFIPFAGTVAKHFTNPISRIIFLLYWFYIILFMPRFVFPPRYAGPMIYQWMIESGFIFSLALFLLVVVAMRTWHLSWPGIAPRYQEGTRWWIVLLLILIPLIYISLISVTPDYQEVLHKLMTFNFHELGINKLFLLTALRAGISEEVIFRFGLIGVLLGALKNHRGRIAWSLLISSLIFGGMHLINLRLQPIGTTLLQVAFAFGLGLLFACVYLYTGQLWLTILFHFLVDFPSFVISHGIVSTATVSSASLITITILVLIFASPLWWLLVGKNRLVMQRHADHLSGTNQHFDYKMNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJD4</accession>
    <name>H4GJD4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Geranyltranstransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_11045</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000035">
        <property type="protein sequence ID" value="EHS86883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122167.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000035.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11045"/>
    <dbReference type="EnsemblBacteria" id="EHS86883">
        <property type="protein sequence ID" value="EHS86883"/>
        <property type="gene ID" value="PS3_11045"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.842"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="289" mass="31030" checksum="034FDF4DBA3DDD51" modified="2012-04-18" version="1">MQTLSELQAQVNQYLLTNLADDIDEATLSAAMNYSVDAGGKRLRPTMTLMVAQILGVELNSDVLRAACALELLHTYSLIHDDLPAMDNDDLRRGKPTSHKQFGEAIAILAGDGLLTLAFQWLTDNQLSANVKARLTLELSKAAGPVGMVAGQVRDILSTGKTLSIERLQKLHAQKTGALLKYATVAGGVIAEVGDEVVAELANFGALYGLAFQIYDDILDVTATSADLGKATHKDAERGKNSYPALLGLPGAEKKLSQVLTAAKVSQERLTTLTQADFSRLDELLAYYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJD7</accession>
    <name>H4GJD7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription antitermination protein NusB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Antitermination factor NusB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nusB</name>
        <name evidence="8" type="ORF">PS3_1456</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in transcription antitermination. Required for transcription of ribosomal RNA (rRNA) genes. Binds specifically to the boxA antiterminator sequence of the ribosomal RNA (rrn) operons.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the NusB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000035">
        <property type="protein sequence ID" value="EHS86886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122170.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000035.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_1456"/>
    <dbReference type="EnsemblBacteria" id="EHS86886">
        <property type="protein sequence ID" value="EHS86886"/>
        <property type="gene ID" value="PS3_1456"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.846"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00073">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011605">
        <property type="entry name" value="NusB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11078">
        <property type="entry name" value="PTHR11078"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01951">
        <property type="entry name" value="nusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="4 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NusB" evidence="7">
        <location>
            <begin position="7"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="138" mass="15521" checksum="8DEFB9FC737E6C5E" modified="2012-04-18" version="1">MSFSRHRLREAAFQTLFALDASPEIDQYPIFQEVLALNPKEPVPEFAQQLVDGVLSHQDELDEKISASLAKGWTLARVARPNVIILRLAIYELEYDQSTPAPVVINEALQLAKTFSDDKSRRFVNGVLGNITNHQTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJE3</accession>
    <name>H4GJE3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_11043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000035">
        <property type="protein sequence ID" value="EHS86892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122176.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000035.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11043"/>
    <dbReference type="EnsemblBacteria" id="EHS86892">
        <property type="protein sequence ID" value="EHS86892"/>
        <property type="gene ID" value="PS3_11043"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.853"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd16332">
        <property type="entry name" value="Prp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007422">
        <property type="entry name" value="Peptidase_Prp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036764">
        <property type="entry name" value="Peptidase_Prp_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39178">
        <property type="entry name" value="PTHR39178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04327">
        <property type="entry name" value="Peptidase_Prp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118010">
        <property type="entry name" value="SSF118010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="108" mass="12041" checksum="F139280F6C0F7A7F" modified="2012-04-18" version="1">MIRATFWLTDDQRVISFKLTGHADAGPYGQDIVCAAVSVLAISSVNGLEQVVHTQPEIVQDQNNGGYLEVKDIDLGHDAQIIMQTFFYGILDIQDNYSDHIEVKVFND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJE9</accession>
    <name>H4GJE9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Prophage pi1 protein 44, tail component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_21571</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000036">
        <property type="protein sequence ID" value="EHS86872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21571"/>
    <dbReference type="EnsemblBacteria" id="EHS86872">
        <property type="protein sequence ID" value="EHS86872"/>
        <property type="gene ID" value="PS3_21571"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.872"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="522" mass="57654" checksum="5CC3FECDFE61C6D0" modified="2012-04-18" version="1">MLASQAKIAFSAIASTDSKGLTKAFKGLMSVMTSQLRDFGRAFTKNWKNNWNKLDKPITNGMKKVRSTIKGELSDLGKIATNFEGDFLDDWRDWVSDIDQSFKDGFNKLPKYAESSMNKIIDKLNNGIKGINSVISDFGGDKKLTTIRYANGTQGGHPGGHMIVNDSVRPSWKELVKFPGQPWTMFNSRNVLIPNAPAGTQVLSGEQTKQVMNEAGIYHYADGSLSEKEQDIISQAFISNPKKAAKGLMLKYTNWDSNIDVVAKLGKASAIAFSQGIANVLKDLLGIIKEPINGDWTPVIKSAFHYLHKTAEAYKVQKLLRQIKTESNGNEKITQHISDVNSRNGNPAKGLLQFIPQTFSTWAVSGHTNIWSGFDQILAAINALDHGGEGGWSNVGNGHGWANGGRVQYHGMYEVAEGNLPEYIIPMDMNKRPRAYQLLGEMTAQMAQEDGWKFKEFSRTSSDKNPQTAGIVNRLDQILNVLASIAGLAGEQIQAIKDQGVFDSDAFNRKQARNIRMKQFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJF1</accession>
    <name>H4GJF1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prophage L54a, major capsid protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_16995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000036">
        <property type="protein sequence ID" value="EHS86874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122184.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000036.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16995"/>
    <dbReference type="EnsemblBacteria" id="EHS86874">
        <property type="protein sequence ID" value="EHS86874"/>
        <property type="gene ID" value="PS3_16995"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.879"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024455">
        <property type="entry name" value="Phage_capsid"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05065">
        <property type="entry name" value="Phage_capsid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01554">
        <property type="entry name" value="major_cap_HK97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="376" mass="41770" checksum="9F243D96200AB3E0" modified="2012-04-18" version="1">MNFYQMKRNAMNIGDELQQANEKLNDMLNNPQISTEDITAQQKVVDTVQQRFKIANAQLAKEEAKQKDNLVKESNQMDPQEKRKSAFAELVRKTMAKESVPASIYQALGDNDSTGGNKFLPKTVSTNIITGPQEHNPLRDISTVTQITNLEIPRLSFTLDDDGFIQDGDTAKEIQAKGDTVQFTRNKFKVKVGLSETVLLGSDANLAAYVENELTNGVTRKERNVAFAESPAKENEKHMSFYDASVGIKSVSGSDLYDAITNAVADLHEDYRENATIVMAYKDYLKIIKTLANGSTTLYGAQPEAVLGKPVVFTDAATTPVVGDFSYSQYNYDINTIYDEDKDVDTGIEKFVLTAWFDHQIKLSSAFRLAKVTPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJF4</accession>
    <name>H4GJF4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Prophage Lp1 protein 24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_15655</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000036">
        <property type="protein sequence ID" value="EHS86877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122187.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000036.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15655"/>
    <dbReference type="EnsemblBacteria" id="EHS86877">
        <property type="protein sequence ID" value="EHS86877"/>
        <property type="gene ID" value="PS3_15655"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.894"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008822">
        <property type="entry name" value="Endonuclease_RusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036614">
        <property type="entry name" value="RusA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05866">
        <property type="entry name" value="RusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103084">
        <property type="entry name" value="SSF103084"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="138" mass="15978" checksum="EF0944866053B866" modified="2012-04-18" version="1">MKFVFDIEPVAQARPRATRFGKSIRLYDPKAVKVYKQSLAYLAQNAYHGKPLSGPLRVILRFYRSIQRSVSKKEHTRRDNGQIRPTVKADVTNYAKSTEDALNGILWKDDAMIVDEFIYKYYSDHPRVEIEVIEIGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJG7</accession>
    <name>H4GJG7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Endopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_15352</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000039">
        <property type="protein sequence ID" value="EHS86610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122200.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000039.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15352"/>
    <dbReference type="EnsemblBacteria" id="EHS86610">
        <property type="protein sequence ID" value="EHS86610"/>
        <property type="gene ID" value="PS3_15352"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.918"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08662">
        <property type="entry name" value="M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018497">
        <property type="entry name" value="Peptidase_M13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042089">
        <property type="entry name" value="Peptidase_M13_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008753">
        <property type="entry name" value="Peptidase_M13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11733">
        <property type="entry name" value="PTHR11733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01431">
        <property type="entry name" value="Peptidase_M13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05649">
        <property type="entry name" value="Peptidase_M13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00786">
        <property type="entry name" value="NEPRILYSIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M13_N" evidence="9">
        <location>
            <begin position="10"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M13" evidence="8">
        <location>
            <begin position="440"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS86610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="633" mass="71660" checksum="E1FA652F6DF2661C" modified="2012-04-18" version="1">MTVDLQAVKTDLYEAVNGEWAKTAQIPADRPATGGFQDLVIDIEKLLMNDLEKFASGEKELPNEQMAQAVKLYQLALDFDRRASEGTVPLQVELDQIEALQSYEDYQSIWHDWLLTGNASPVDFDLDPDMKNAKVYGLYAGAPSLILPEKNYYADDNQQGQALLQVWTTMAEKVLVMVGYDQEIAQKLVTETKVFDARLVPHVKSAEERADYSKMYNPMTVNEFAQATDQLSLSEIIKSLIKTTEAKVIVTEPAYFQALDQILTDHFTEFKSWLTVKTILGDASVLTDDLRIASGEYSRTLSGSQEAVNHQKFAYYFTMSYFGQVIGKYYGETYFGPEAKANVEYMVHQMIKVYQERLENNDWLSPATRQQAIVKLTHLGVQVGYPEKLPAYYEQLKIDTGKSLLANAITLSQQLKAESFSRWNQPVDQTRWEMSAATVNAYYHPFMNIIVFPAAILQAPFYSLQQTSSQNYGGIGAVIAHEISHAFDNNGSLFDEYGNLKNWWTEEDQSHFKALAEKMIKEFDGLPYAGQSVNGKLTVSENIADAGGLSCALSAAQEEADYYAQAFFLNWARVWRMKATEQYMQLLLSIDVHAPNKLRANVQAQNLAEFYLAFDVQSGDQMYLEPDQRVQIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJK0</accession>
    <name>H4GJK0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Protein-N(Pi)-phosphohistidine--sugar phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PS3_14886</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122233.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14886"/>
    <dbReference type="EnsemblBacteria" id="EHS86510">
        <property type="protein sequence ID" value="EHS86510"/>
        <property type="gene ID" value="PS3_14886"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.972"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00212">
        <property type="entry name" value="PTS_IIB_glc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036878">
        <property type="entry name" value="Glu_permease_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018113">
        <property type="entry name" value="PTrfase_EIIB_Cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001127">
        <property type="entry name" value="PTS_EIIA_1_perm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003352">
        <property type="entry name" value="PTS_EIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013013">
        <property type="entry name" value="PTS_EIIC_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011297">
        <property type="entry name" value="PTS_IIABC_b_glu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001996">
        <property type="entry name" value="PTS_IIB_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00358">
        <property type="entry name" value="PTS_EIIA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00367">
        <property type="entry name" value="PTS_EIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02378">
        <property type="entry name" value="PTS_EIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55604">
        <property type="entry name" value="SSF55604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00830">
        <property type="entry name" value="PTBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01995">
        <property type="entry name" value="PTS-II-ABC-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51093">
        <property type="entry name" value="PTS_EIIA_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00371">
        <property type="entry name" value="PTS_EIIA_TYPE_1_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51098">
        <property type="entry name" value="PTS_EIIB_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01035">
        <property type="entry name" value="PTS_EIIB_TYPE_1_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51103">
        <property type="entry name" value="PTS_EIIC_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="313"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="400"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIB type-1" evidence="14">
        <location>
            <begin position="7"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIC type-1" evidence="15">
        <location>
            <begin position="109"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIA type-1" evidence="13">
        <location>
            <begin position="532"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate; for EIIB activity" evidence="11">
        <location>
            <position position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EHS86510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="661" mass="70413" checksum="97EF9E3333BEA572" modified="2012-04-18" version="1">MADNGYRDLAQFIIDNVGGKENIISVVHCTTRLRFKLKDEAKANDDQLKANDGIVTVVKSSGQYQVVIGTHVDKVYDDVLAVGGLTGGGQVADDYEDKSNMSLMDRFIDLISGIFTPFLGVMAAAGMIQGLTSAMGSFGWISTTSGTYTILYAIGNGFFYFLPIILGLTAARKFKVNEFIGIAIGAAMVYPNILSLYNSKTVLMTLFKGSIIESPIHATFLKIPVILMNYSSTVIPIVLAVWFASYVQKFVTKLIPGVVQLFLVPFFTLLITVPITFLVIGPVATWIADIIAAAIQAVINFSPILAAVIVGGFWQVLVMFGVHWGIVAAFYTELSSQGWDQMLLFSVSVCFSQIGVVTAIIFQTKNPKTKAIALPALISGIFGVTEPAIYGVTLPRKKSFILSCIGGAIGAIPFALGGVKAFSMAGMGVFAFPMVIGKSHVTSSLIWWLISVIVSYLAGLLLQFIFGKSAVDSKEELAELQAAAVTEVGAQAADVKRGAAEQTAKDAAEYNQPTKLYAPVNGQTLPLTEVKDEVFSSEAMGKGLAFVPSEGLLRAPADGTVVLVFPTGHAIGMQTAAGAEILMHIGMDTVNLQGKGFETLVEKGQAVKAGDPLVKFNLAAIKQAGYDPTMPMVVTNSVKYHEVKAVAKDTVAVGDEVLELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJL4</accession>
    <name>H4GJL4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Arsenate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_3771</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the ArsC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122247.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3771"/>
    <dbReference type="EnsemblBacteria" id="EHS86524">
        <property type="protein sequence ID" value="EHS86524"/>
        <property type="gene ID" value="PS3_3771"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.992"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006660">
        <property type="entry name" value="Arsenate_reductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006504">
        <property type="entry name" value="Tscrpt_reg_Spx/MgsR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30041">
        <property type="entry name" value="PTHR30041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03960">
        <property type="entry name" value="ArsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01617">
        <property type="entry name" value="arsC_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51353">
        <property type="entry name" value="ARSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="118" mass="13899" checksum="C079336F4A89C76A" modified="2012-04-18" version="1">MTTFYCYSRCSTCKKAQKWLDEHQVAYDQQDLVEQPPTKEQFVDWLTNSGHPIKYFFNTSGQHYRQQNLKERVNDLTIDEAATLLSTDGKLIKRPLMVDGHQLTCGFKTDIYEETWLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJN5</accession>
    <name>H4GJN5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_21944</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000045">
        <property type="protein sequence ID" value="EHS86236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122268.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000045.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21944"/>
    <dbReference type="EnsemblBacteria" id="EHS86236">
        <property type="protein sequence ID" value="EHS86236"/>
        <property type="gene ID" value="PS3_21944"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1023"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009214">
        <property type="entry name" value="DUF1129"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06570">
        <property type="entry name" value="DUF1129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033111">
        <property type="entry name" value="UCP033111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="259" mass="28488" checksum="E2082C244897E2AC" modified="2012-04-18" version="1">MAEQPRNAAAGEKQRQYKKTEVKSIADQFEQAGLTRKNIDYMIQLNKQLDLQGADPDAKATMLEQTVMKLTESQKQGTTARTLLGTPTEYADQLIHPAPSAKQVERSASGLLAIDNALMFFAIFTVMFGIMGLSQPKSLASSNGNAGIVSILIVSIVGGLLFGYMTRLLTSTKDPKTGKRTGYPLWLRALLVVVAFVVWVAIYGLSAFIPKTINPVLNGWVYIVLAVVAFFGDMYFRRRYNVVGGFMGRPANQGQNQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJN8</accession>
    <name>H4GJN8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Stage 0 DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_10540</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ParB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000045">
        <property type="protein sequence ID" value="EHS86239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122271.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000045.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10540"/>
    <dbReference type="EnsemblBacteria" id="EHS86239">
        <property type="protein sequence ID" value="EHS86239"/>
        <property type="gene ID" value="PS3_10540"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1027"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041468">
        <property type="entry name" value="HTH_ParB/Spo0J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004437">
        <property type="entry name" value="ParB/RepB/Spo0J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17762">
        <property type="entry name" value="HTH_ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00180">
        <property type="entry name" value="parB_part"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB" evidence="4">
        <location>
            <begin position="20"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="238"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="317" mass="35562" checksum="D0E2A1CCC250679A" modified="2012-04-18" version="1">MAFSIFNLGKDDEHVKNQVVEVPLKDIVPNRYQPRKVFDRQAIEELAQTIREHGLLQPIIVREYEAGKYEIIAGERRFRAMTSLNWETAPAIVEKMSDEETASLALIENLQRSNLSPVEEAQAYRKLMDLNHLTQRTLAEGMGKSQSFVANKLRLLQLAEPVQTAILNHQITERHGRALLNLKQVDQAAMLAEVISHEWTVRQTEDAVAAKLGQPLPSVIRARKQREIYGIGADESADVTATTEDPKVNPKKKSTRRTNKHAPKITNAQSAAKAIQAAVDNLKGTGYHTEVTTQDTENGVQVIIQVVDQPRKRGRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJR4</accession>
    <name>H4GJR4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycerate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_7295</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycerate kinase type-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000047">
        <property type="protein sequence ID" value="EHS86214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122297.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000047.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7295"/>
    <dbReference type="EnsemblBacteria" id="EHS86214">
        <property type="protein sequence ID" value="EHS86214"/>
        <property type="gene ID" value="PS3_7295"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1070"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008887">
        <property type="term" value="F:glycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031388">
        <property type="term" value="P:organic acid phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018193">
        <property type="entry name" value="Glyc_kinase_flavodox-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004381">
        <property type="entry name" value="Glycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018197">
        <property type="entry name" value="Glycerate_kinase_RE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036129">
        <property type="entry name" value="Glycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21599">
        <property type="entry name" value="PTHR21599"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02595">
        <property type="entry name" value="Gly_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006078">
        <property type="entry name" value="GlxK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110738">
        <property type="entry name" value="SSF110738"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00045">
        <property type="entry name" value="TIGR00045"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR006078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="378" mass="38511" checksum="9F84C759E24CFC81" modified="2012-04-18" version="1">MKFVIAPDSFKESMTALVAAQAIQTGLLRALPTAQCQLIPLADGGEGTVATLVHAHQGEMVLTSTAGPLPGQMIQGAYGLIDNGKTAVIEVAAADGLTLVPRQQRDPLLTSSYGTGQQILAALDRGVQRIIIGLGGSASVDGGLGILQALGARCLDDQGQPVPAGGQGLSQVATIDLSTIDPRLSQTELILASDVTNPLLGPHGAAKVFGPQKGATPKMVATLEAGMKNFARRMTVTVGRDFSQAAGSGAAGGIGFALLVLGGQLQSGIELMMAATNLSQAIQAADLVITGEGRIDGQTLFGKVPAGVARLAKQAHKPLIAIGGSLGDDLSVLYDMGFTAIFACLGSVEPLDQVLVQGPANLSRTAENIGRILVLESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJU0</accession>
    <name>H4GJU0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribonuclease 3</fullName>
            <ecNumber evidence="9">3.1.26.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Ribonuclease III</fullName>
            <shortName evidence="9">RNase III</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rnc</name>
        <name evidence="13" type="ORF">PS3_11243</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribonuclease III family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.3" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122323.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11243"/>
    <dbReference type="EnsemblBacteria" id="EHS86127">
        <property type="protein sequence ID" value="EHS86127"/>
        <property type="gene ID" value="PS3_11243"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1118"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00104">
        <property type="entry name" value="RNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011907">
        <property type="entry name" value="RNase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="PTHR11207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="SSF69065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02191">
        <property type="entry name" value="RNaseIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="9" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="RNase III" evidence="12">
        <location>
            <begin position="6"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="11">
        <location>
            <begin position="161"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS86127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="233" mass="26550" checksum="CDE9D03001997E23" modified="2012-04-18" version="1">MITELSEYLSSEFGIQFKNLDLLAEAFTQASYVNEHPKEYLKFYERIEFLGDAVLQLYVSEYIYQRYPELPQGKLTRLRAAMVQEKSFAAFARECHFDQYIRLGKGEEMAGARNRDSLLCDIFESFIGALYLDQGREAVKKFIARVIFPKLDLGWFDHAVDSKTSLQEFLQRDGDVDIEYHLLSEGGTENAPEFTVNVSMDGQVIGEGTGSSKKHAEMAAAQAALKKLRQDKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJU6</accession>
    <name>H4GJU6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TPK_B1_binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_17517</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122329.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17517"/>
    <dbReference type="EnsemblBacteria" id="EHS86133">
        <property type="protein sequence ID" value="EHS86133"/>
        <property type="gene ID" value="PS3_17517"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1124"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030975">
        <property type="term" value="F:thiamine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07995">
        <property type="entry name" value="TPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006282">
        <property type="entry name" value="Thi_PPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007373">
        <property type="entry name" value="Thiamin_PyroPKinase_B1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007371">
        <property type="entry name" value="TPK_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04265">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04263">
        <property type="entry name" value="TPK_catalytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00983">
        <property type="entry name" value="TPK_B1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="SSF63999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01378">
        <property type="entry name" value="thi_PPkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TPK_B1_binding" evidence="5">
        <location>
            <begin position="146"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS86133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="216" mass="24469" checksum="D5674A0D12E0DB40" modified="2012-04-18" version="1">MGRVVNVLVGGPEHLIPYTDFARLDGLWIGVDLGATRLIDHQIIPDIAVGDFDSSDDQQFKRVKELVSDVRFYPPEIKTEDTDTQLAIRIALELGADQIRLYGATGGRLDQYLANLYTVLMPQYRPALAKLAIIDRGNHITWYEPGTYQINRLPEMRYLAFVNLTPVTGLTLPDEKYQLYDYNATFPISWSSNEFVGNQAHFSFETGVIMVIQSHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJW4</accession>
    <name>H4GJW4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lantibiotic transport ATP-binding protein srtF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_2018</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000050">
        <property type="protein sequence ID" value="EHS86038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122347.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000050.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2018"/>
    <dbReference type="EnsemblBacteria" id="EHS86038">
        <property type="protein sequence ID" value="EHS86038"/>
        <property type="gene ID" value="PS3_2018"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1148"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="303" mass="33417" checksum="1D8F2256218EF28C" modified="2012-04-18" version="1">MTSILSVKQLNKNFKKRQVLFDVSFDCKMGRIVGLIGPNGVGKITIMKSVLGLIKSSGSIKIDGKEVSFNNGPVAGTVGALIENPGIYPYLSGLEHLKMFSKKRSQDDINQLVSDLGMSGYIQKRASNYSLGMKQKLGIAMALINNPKLVILDEPMNGLDPQATKELRNLIIRKASEGMTFLISSHLLSELQKLADDVILINKGKIIRNDTMENLLATNKRVAILKTNDDSAAQTILSEVGFEFDTKAVFEPGTDKKPLRILVADDKQVEYAVTLLQKNNIIVLDIHHEDSDLETTVLNLMDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJX0</accession>
    <name>H4GJX0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_18165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS85987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122353.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18165"/>
    <dbReference type="EnsemblBacteria" id="EHS85987">
        <property type="protein sequence ID" value="EHS85987"/>
        <property type="gene ID" value="PS3_18165"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1158"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="8"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="221" mass="24287" checksum="337C99EAA24B208D" modified="2012-04-18" version="1">MNLLILAANGQIARLVEERILSEDNFKSVNLTLFLRNADRLANLATNPRVTLIEGDLNKEADVVNAAQGQDLIFSAVVDHDDTGSNRPTKNIIKAAQENQVHRIIETSLLGLYNEVPGEFGRWNNETCFGGDPSGSAPVLADQLLKDSGLNYTTLRLPWLNDRDEIKYTITHRNEPYVGVSGSRKSIADVVLKIVADPNFAAFDSIGIADPDTQGLDRPVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJX3</accession>
    <name>H4GJX3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Major facilitator superfamily transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_9006</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS85990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122356.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9006"/>
    <dbReference type="EnsemblBacteria" id="EHS85990">
        <property type="protein sequence ID" value="EHS85990"/>
        <property type="gene ID" value="PS3_9006"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1162"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="17"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS85990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="438" mass="48187" checksum="70FE463CA0E212C4" modified="2012-04-18" version="1">MTNQQPIARTERKIASNILKGSLGNMIEWFDWYVYSAFAIYFSAAFFPSNNQTAELLSTAAVFAIGFVMRPFGSFVMGRYADRHGRRAALTLSVMIIAGGSMIIALVPTFSQIGIWSPIILVATRMVQGLSLGGEYGTSATYLSEMASPQRRGFYSSFQYVTLIAGQLLALLVQIVLQQMFNEQQLYAFGWRIPFVIGAIGALVVLWLRLTMEESDQFKHANKHNKRSGTLRLLAKYPKQVLIVIGLTLGGTISFYSYTTYLQQFMINTAGMNKEVVAIVNFAALLVMIVLQPVFGALSDHIGRKPLLVFFGVGGTIATVPIFTLLAHVTTAWGAFLLMVSGLILVSGYTSINAIVKAELFPAEIRALGVGLPYSLTVAIFGGTANYVALWMRSAGIESGFYWYVTATIFISLLVYIFGLSHKNDSLIDQEEAEMYGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJX6</accession>
    <name>H4GJX6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">XRE family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_12420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS85993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122359.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12420"/>
    <dbReference type="EnsemblBacteria" id="EHS85993">
        <property type="protein sequence ID" value="EHS85993"/>
        <property type="gene ID" value="PS3_12420"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1169"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="10"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="292" mass="33442" checksum="78B9CE4BCB71F491" modified="2012-04-18" version="1">MDINRFVSRRKELGFSQVQLAQGICTQATLSKFENNGQIPSLNILQQLCNRLGLSLDELSQNDKGSIVYLNRQLKQAEIDLMIGNLAAVQKCLSNISINSQSTLIHQMQFYLIRGLLKLLLDHDHEGVITDFKLVTEELDQEQSTIWYYLGQAGLALVYDREQNAIAAKERFKLVATYVDQLNDTAVENITDFEYVLAISYILGRYYWQHGLLPTAENYIKFGLQLARYHHVTYFVPRLYMVQTEILKAQGADLLTIQATIRQAAVFAEFNHNQVVQKWVQEQGKGELYDAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJZ9</accession>
    <name>H4GJZ9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tyrosine permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_11012</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000052">
        <property type="protein sequence ID" value="EHS85881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11012"/>
    <dbReference type="EnsemblBacteria" id="EHS85881">
        <property type="protein sequence ID" value="EHS85881"/>
        <property type="gene ID" value="PS3_11012"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1215"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="346"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS85881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="379" mass="42648" checksum="6F9F34BA93EDE7DF" modified="2012-04-18" version="1">MWVQMFPAMVMIASTLGPLLGNTIDNRALGQNHWFTLICILVFYWLVTILNLKFDMAKLGGAVGIWLGVYLPISFMILTGFLAFLKLGISPTSILGSFKPSKLVPDLSDYQSLKYISAVVFIFTGISTTGVYAPRLKNLTKTFTRGLSIAVLVIVLMNFFNSFLIANAIPHGQAELTDITQPVIIYCQILHWPSWIVQIFSGFAFIGVLVQLSTWVTAPSQTIIEVANDGLLPPKWGWYRHNQYGVSKNIVLTQAIAITLFALLYALPNINSVFLMLVNTAVFLYCMVYIIIAIAYLILRYQYHGERPFNVDRHGNSRAWLVAILLILGIIICFISTIITTKVLDSVIMISISILLFVIPLIVERYKKPSWLNDLHRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK05</accession>
    <name>H4GK05_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phospho-glucosyltransferase EpsE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_4726</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial sugar transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000053">
        <property type="protein sequence ID" value="EHS85877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122388.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000053.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4726"/>
    <dbReference type="EnsemblBacteria" id="EHS85877">
        <property type="protein sequence ID" value="EHS85877"/>
        <property type="gene ID" value="PS3_4726"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1227"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003362">
        <property type="entry name" value="Bact_transf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02397">
        <property type="entry name" value="Bac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Bac_transf" evidence="3">
        <location>
            <begin position="26"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="219" mass="25512" checksum="DD40141714C36C54" modified="2012-04-18" version="1">MEVRNIEIPIIDKSQVESRCVYRFFKRFFDIVFSLIGLFCLSWLFLIIIIWIKIDDRGPVFFSQTRVGKDGKEFKMYKFRSMVTNAEELKHELMKQNEVDGAMFKMKDDPRITKAGKFIRKTSLDELPQLWNVLIGDMSLVGPRPPLPAEVAHYSEHDMQRLMVKPGCTGLWQVTARNTVGFKDMVGIDLTYIQRANLMYDFWILLCTVKVVIIPNGAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK20</accession>
    <name>H4GK20_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Catabolite control protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_7779</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000054">
        <property type="protein sequence ID" value="EHS85872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7779"/>
    <dbReference type="EnsemblBacteria" id="EHS85872">
        <property type="protein sequence ID" value="EHS85872"/>
        <property type="gene ID" value="PS3_7779"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001761">
        <property type="entry name" value="Peripla_BP/Lac1_sug-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00532">
        <property type="entry name" value="Peripla_BP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="6"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="220" mass="24131" checksum="73F336DB96AAB00A" modified="2012-04-18" version="1" fragment="single">MKKQPVTIYTVAHEANVSMATVSRVVNGNTNVKPETRDKVLEVIKRLNYRPNAVARGLASKKTTTVGVVIPKITDDYFAELAAGIDDVAAIYKYDMILTSLDTVEQQPVQVIQSMLAKQVDGIIFMGHDLSDDLKKEFDNSAIPVLVSGSVVDDDSLPSVRIDYRQATKDSVNFLAQHGHQKIGLLVGYDQTPINAQNRIPAYQEALEAQGLAFDPANVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK32</accession>
    <name>H4GK32_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_15780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000055">
        <property type="protein sequence ID" value="EHS85848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15780"/>
    <dbReference type="EnsemblBacteria" id="EHS85848">
        <property type="protein sequence ID" value="EHS85848"/>
        <property type="gene ID" value="PS3_15780"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1264"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="59"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="155" mass="17339" checksum="04DCE2135DDD6BCE" modified="2012-04-18" version="1">MAKDVWDENEASTSDQARDAYQKSLFFPIGNPNDAYAKYFVGQSYLASVCEDPFPIHNVTFEPGCRNNWHIHHADKGGGQALICVGGRGWYQEWGRDPVEMTPGTVIEIPAGVKHWHGAAADSWFSHLAFGIPGENPSNEWLEAVSDEQYQQLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK33</accession>
    <name>H4GK33_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Formate--tetrahydrofolate ligase</fullName>
            <ecNumber evidence="6">6.3.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Formyltetrahydrofolate synthetase</fullName>
            <shortName evidence="6">FHS</shortName>
            <shortName evidence="6">FTHFS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fhs</name>
        <name evidence="7" type="ORF">PS3_14640</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-formyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20221"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the formate--tetrahydrofolate ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.3" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000055">
        <property type="protein sequence ID" value="EHS85849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122416.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000055.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14640"/>
    <dbReference type="EnsemblBacteria" id="EHS85849">
        <property type="protein sequence ID" value="EHS85849"/>
        <property type="gene ID" value="PS3_14640"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1265"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004329">
        <property type="term" value="F:formate-tetrahydrofolate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00477">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01543">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000559">
        <property type="entry name" value="Formate_THF_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020628">
        <property type="entry name" value="Formate_THF_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01268">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00721">
        <property type="entry name" value="FTHFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00722">
        <property type="entry name" value="FTHFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 6" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="63"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="553" mass="59659" checksum="BCB64DAAC617BEA9" modified="2012-04-18" version="1">MPTDLEIASQAKMQPISEIAASIGLSADDIEQYGKYKAKINLPVKETPDKKHKLVLVTSINPTPAGEGKSTILVGLGDALAKLNHQTIIAMREPSMGPVFGVKGGATGGGYSQVVPMEDINLHFTGDFHALTSANNTLAALIDNYIMRDNALGLDPRRIIWKRVEDVNDRALRNVVTGLGGLMQGVPRETGFDITPASELMAILCLSTDLMDLKRRVNQIVVGYTYQKEPVTVEQLGFADAITILLKDAIKPNLVQTLAHTPAIVHGGPFANIAHGCNSVLATKTALQLADYTVTEAGFGADLGAEKFMDIKTRALDKHPDAVVVVATVRALKYNGGAQLADLQNEDLAALQNGLKNLNRHIQNVQRFGVPMVVAINHFVSDTDAELAMITENCQSLGVNVVTADAWAKGGEGTLDLAEEVVRLADQPADFHHLYSLDQTIEEKVTTIATKVYGAARVEFSKKAKNQLKQFADRGWNDMPVCIAKTQYSFTDDQTQLGAPTDFVFHIREFVPKLGAGFVVALSGNMLTMPGLSKTPAAVKMTIDEEGMITGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK34</accession>
    <name>H4GK34_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_15232</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000055">
        <property type="protein sequence ID" value="EHS85850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122417.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000055.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15232"/>
    <dbReference type="EnsemblBacteria" id="EHS85850">
        <property type="protein sequence ID" value="EHS85850"/>
        <property type="gene ID" value="PS3_15232"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1266"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="348" mass="39891" checksum="DA592026F34A6AFE" modified="2012-04-18" version="1">MESSWQRFINNTILRRTVVLVAIIFVLWYVRAVMTEILLTFIFTYLIVHWLRLVQRWLPKLPTAITAVVTYAIIIFLAYVGVTKYMPILINQIVRMVDYVINFSQSNDMTWFTKYITHYVSMSTITTQVRHWVTIGISTLTSVGTFSFSFVMAVILSFFYSIELKQMNGFSRSFLDSNVAGWFFRDLYYFGQKFVNTFGVVLEAQFFIAITNTVITTVCLIVMQMPQIVALSVMVFAFSLVPVAGVIISLIPLCMVGYSTGGIKYIIYILIMIAVIHVIEAYVLNPKFMASRTQLPIFYTFVVLLVGEHFFGVWGLIVGVPIFTFFLDILGVKSFTKSKTNKINLQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK50</accession>
    <name>H4GK50_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-adenosylmethionine synthase</fullName>
            <shortName evidence="11">AdoMet synthase</shortName>
            <ecNumber evidence="11">2.5.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">MAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Methionine adenosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">metK</name>
        <name evidence="17" type="ORF">PS3_6169</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + H2O + L-methionine = diphosphate + phosphate + S-adenosyl-L-methionine</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="11">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 divalent ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11 13">Belongs to the AdoMet synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="11"/>
    <dbReference type="EMBL" id="AICN01000057">
        <property type="protein sequence ID" value="EHS85803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122433.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000057.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6169"/>
    <dbReference type="EnsemblBacteria" id="EHS85803">
        <property type="protein sequence ID" value="EHS85803"/>
        <property type="gene ID" value="PS3_6169"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1293"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00086">
        <property type="entry name" value="S_AdoMet_synth1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="PTHR11964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000497">
        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="SSF55973"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="10 11 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-AdoMet_synt_N" evidence="14">
        <location>
            <begin position="5"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="S-AdoMet_synt_M" evidence="15">
        <location>
            <begin position="124"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="S-AdoMet_synt_C" evidence="16">
        <location>
            <begin position="244"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Flexible loop" evidence="11">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="174"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="241"/>
            <end position="242"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="256"/>
            <end position="257"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EHS85803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="395" mass="42950" checksum="C9CBA891FDB16023" modified="2012-04-18" version="1">MKEKHLFTSESVSEGHPDKIADQISDSILDALLAQDPQARVAVETSVTTGLVLVFGEVSTSAYVDVQSVVRNTIKEIGYTDGKYGFDGDNCAVIVALDEQSSDIAQGVDDSWEIRDGETDSLDQIGAGDQGLMFGYATNETPEFMPLPLILSHKLMRKIAQLRKDRVIPYLRPDAKAEVTVEYDDNDQPVRIDTVVLSTQHDPEVSLEQIQADVKEQVVKAVIPNELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGAAHHGGGAFSGKDATKVDRSASYAARYIAKNLVAAGYADKLEIQIAYAIGVATPVSISIDTFGTGKKSEAELIDAVRQTFDLRPAGIIKMLDLQRPIYKQTAAYGHFGRTDIDLPWEQLDKVDALKSILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK55</accession>
    <name>H4GK55_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Restriction endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_20817</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000059">
        <property type="protein sequence ID" value="EHS85689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122438.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000059.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20817"/>
    <dbReference type="EnsemblBacteria" id="EHS85689">
        <property type="protein sequence ID" value="EHS85689"/>
        <property type="gene ID" value="PS3_20817"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1300"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025745">
        <property type="entry name" value="Mrr-like_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007560">
        <property type="entry name" value="Restrct_endonuc_IV_Mrr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04471">
        <property type="entry name" value="Mrr_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14338">
        <property type="entry name" value="Mrr_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mrr_N" evidence="7">
        <location>
            <begin position="11"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Mrr_cat" evidence="6">
        <location>
            <begin position="154"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="295" mass="33946" checksum="138E3274D18DB78E" modified="2012-04-18" version="1">MANQRIMIEDQTMNEVLKSMYRLGGQVSRKEIRQDIHDNSEVISEDEVDRIKVSKKTGKNYSPFAWTLNFAVKYLIMTGYLHEDGHLLELTEKGRNVDVAAFDGARDVRPVVEKMLAQEKQNKVTSSDKINTDDHEDPDELEEPWRQQLLDALTKMNPQKFELFCRGLLTKMGINTDDELGVQYVADGGIDGFGYITADDFRTTRVALQAKRWQGKVSAPEIDKFRGAMDKFNAEFGIFITNSDFTREAVKTARQGSRIITLINGDQICNLVAKYHYYVEPVTTYRLKDFYWDKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK75</accession>
    <name>H4GK75_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nicotinamidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_7481</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000061">
        <property type="protein sequence ID" value="EHS85636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122458.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000061.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7481"/>
    <dbReference type="EnsemblBacteria" id="EHS85636">
        <property type="protein sequence ID" value="EHS85636"/>
        <property type="gene ID" value="PS3_7481"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1326"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="SSF52499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="183" mass="20562" checksum="E44451AB09A5EC85" modified="2012-04-18" version="1">MLADALLIIDYTNDFVARHGALTCGEPAQKIAANLVQLANQYLEAGKWVILPTDVHVADDPYHPETKLFATHNVRDSWGRELYGELDQWYHHHQNDPHVMMMDKTRYSSFAGTTLDLDLRARQVSSVALTGVCTDICVLHTAVDAYNLGYQITVYEDAVATMTEMGQEWALNHFKNVLGAKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK84</accession>
    <name>H4GK84_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_14498</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122467.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS85536">
        <property type="protein sequence ID" value="EHS85536"/>
        <property type="gene ID" value="PS3_14498"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1343"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010619">
        <property type="entry name" value="ThrE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024528">
        <property type="entry name" value="ThrE_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06738">
        <property type="entry name" value="ThrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12821">
        <property type="entry name" value="ThrE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="329"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="419"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="ThrE" evidence="6">
        <location>
            <begin position="43"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="ThrE_2" evidence="7">
        <location>
            <begin position="309"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="445" mass="48590" checksum="D43F9A6B557CCEA0" modified="2012-04-18" version="1">MTATENQTEEYRQHMEMPWEQFVQSNDDVPAKKAALIERTVTVGRIGLMILACGTGSWRVRDAMNIVARSLGLTCTADIGLMTIEYTCVEGAHSYTQVLSLPSSGVNMTRLNRLEGLVKEIELSNRQWTVPEIHDRLSQIEKEKSSYTAIHQGLAAGLACAAFVFLLGGGPIEMLCSFIGAGIGNFVRRQLIARKMMLLVCVSIAVAVACLCYAGAFYLIHALFQVSGRHEAGYIGAMLFIIPGFPFITSGLDMSKLDMRSGLERLSYAILIILVGTLVGWLMAVIVGLKPENFIAITMSPALLMILRLICSFFGVFGFSIMFNSSVRMASVAGLIGAIANTLRLTLVDHQILAGAAAFLGALMAGLLASIIRRRVGYPRISITIPSIVIMVPGLYLYRAVYNFGMMNVGTSAEWMMKAIQVMVFLPLGLTAARVLTDPKWRHTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK89</accession>
    <name>H4GK89_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Citrate lyase subunit beta</fullName>
            <ecNumber evidence="8">4.1.3.34</ecNumber>
            <ecNumber evidence="9">4.1.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Citrate (pro-3S)-lyase subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Citryl-CoA lyase subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PS3_13907</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Represents a citryl-ACP lyase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(3S)-citryl-CoA = acetyl-CoA + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:20812"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57321"/>
            <dbReference type="EC" id="4.1.3.34"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>citrate = acetate + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:10760"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="EC" id="4.1.3.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Oligomer with a subunit composition of (alpha,beta,gamma)6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.34" evidence="8"/>
    <dbReference type="EC" id="4.1.3.6" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122472.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13907"/>
    <dbReference type="EnsemblBacteria" id="EHS85541">
        <property type="protein sequence ID" value="EHS85541"/>
        <property type="gene ID" value="PS3_13907"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1348"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009346">
        <property type="term" value="C:ATP-independent citrate lyase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008815">
        <property type="term" value="F:citrate (pro-3S)-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008816">
        <property type="term" value="F:citryl-CoA lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005000">
        <property type="entry name" value="Aldolase/citrate-lyase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011206">
        <property type="entry name" value="Citrate_lyase_beta/mcl1/mcl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006475">
        <property type="entry name" value="Citrate_lyase_beta_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03328">
        <property type="entry name" value="HpcH_HpaI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015582">
        <property type="entry name" value="Cit_lyase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01588">
        <property type="entry name" value="citE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0456">Lyase</keyword>
    <keyword evidence="13 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpcH_HpaI" evidence="18">
        <location>
            <begin position="7"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16 17">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00012914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00015712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR015582-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR015582-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF03328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EHS85541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="301" mass="33551" checksum="AD764DEE8C5C49D5" modified="2012-04-18" version="1">MEERLRRTMMFVPGNNAGMVKDAGIYGADSIMFDLEDSVSMAEKDAARMLVYEALQTQDYGDAELVVRVNGIDTPYFRNDVFAMVKAGIEVVRLPKVESAQMMLDLVKVVEEAEAKFGREPGSTHVMAAIESARGVLKSLEIAESTDRMIGLALSAEDYTTDMKTHRYPDGAELEFARNMILHAARAAGIAAFDTVFTNMNDVEGFQRETEHIHQLGFDGKSLVNPRQIDWVNKIYEPTRKEIENALDVEAAIEEANQKGSGVISMNGQMVDRPVVLRAQRVIRLAKASHLINEEGEYIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKC1</accession>
    <name>H4GKC1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Beta-galactosidase</fullName>
            <ecNumber evidence="3 8">3.2.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Lactase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_12859</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyl hydrolase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="3 8"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122504.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12859"/>
    <dbReference type="EnsemblBacteria" id="EHS85573">
        <property type="protein sequence ID" value="EHS85573"/>
        <property type="gene ID" value="PS3_12859"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1392"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009341">
        <property type="term" value="C:beta-galactosidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004199">
        <property type="entry name" value="B-gal_small/dom_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036156">
        <property type="entry name" value="Beta-gal/glucu_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006101">
        <property type="entry name" value="Glyco_hydro_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023232">
        <property type="entry name" value="Glyco_hydro_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006103">
        <property type="entry name" value="Glyco_hydro_2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023230">
        <property type="entry name" value="Glyco_hydro_2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006102">
        <property type="entry name" value="Glyco_hydro_2_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006104">
        <property type="entry name" value="Glyco_hydro_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032312">
        <property type="entry name" value="LacZ_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02929">
        <property type="entry name" value="Bgal_small_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16353">
        <property type="entry name" value="DUF4981"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00703">
        <property type="entry name" value="Glyco_hydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02836">
        <property type="entry name" value="Glyco_hydro_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02837">
        <property type="entry name" value="Glyco_hydro_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00132">
        <property type="entry name" value="GLHYDRLASE2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01038">
        <property type="entry name" value="Bgal_small_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49303">
        <property type="entry name" value="SSF49303"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00719">
        <property type="entry name" value="GLYCOSYL_HYDROL_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00608">
        <property type="entry name" value="GLYCOSYL_HYDROL_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bgal_small_N" evidence="9">
        <location>
            <begin position="736"/>
            <end position="1006"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="1009" mass="115608" checksum="8E94DE7991DE0457" modified="2012-04-18" version="1">MIKQNITTADLNWLADPQVDAVNTVPAHSDHHYYASLSELSQGKSTFVQSLDGQWKVNYVRDADLRPRDFYQTDFDDTDFTTITVPGHLELQGYGKPQYVNTQYPWDGGEFLRPPMIPQHHNAVASYIRYFRVNDHLIHDSERVRLVFEGASTAIYVWLNGHFIGYHEDSFTPAEFDITSALVEGENKLAVALYRFSTANWVEDQDFWRLTGLFRSVVLKGQPKVHLEDIKTNATLDDSYQRGILDAQVSLTGPIDANLTVEALLLDRQNKVLASQSQPAKAELNFHFEDLAIEAWSDEQPQLYRLRLAVRKDRFVYEVVPVEVGFRRFELIDRVMYLNGKRLVFRGVNRHEFDARLGRAVTDDDMRWDIQYLKQHNINAVRTSHYPNQSRWYELCDQYGIYVIDEANLESHGTWQKLGECEPSWNIPASEPEWLDTCLYRANNMYQRDKNHPSILIWSCGNESYAGDDIAAMSKFFHEQDPSRLVHYEGVTWNREYDQITDIESRMYAKPAEIETYLKDNPQKPYISCEYMHAMGNSLGGLQLYTALEKYSQYQGGFIWDFIDQGIYQQQANGVERLTYGGDWDDRPADYEFCGDGLVLANRQDTPKGSAVKQLYSPIQIEPDQAGVKIINRRLFTMTDDLYFVATVMQDGHQVWQSEPMNWLVKARTSAHFAIEYPKDLSGEIVYTVTAYLASSKQWAAKGYPIVSGQTVIQESLPKFVASGQPQLIEGDFNYGVKGKNFQALFSRAQGTLVSLKYGGQELIQKLPRLTFFRALTDNDRGAGYGYDFHKWMDLGHYAKMTEVKAELKAQVAQITYQYLLPSQMGKASVCYQVDQTGKINVVAEYDGHLVGEYLPEFGLEFGVPAQLDQFDYYGLGPDDNYWDRQAGVQLGRYQVKVADNNIPYLRPQESGNRMQVRELAVHDGQQHGFKVVADQQVFEANVQSHQTLEYELADHQEELAPSQITWVKLLAVQMGVGGDDSWGATVHAEFMLPADQKYRLSFTIEPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKC9</accession>
    <name>H4GKC9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="4 8">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PS3_13767</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the first step in the biosynthesis of NAD from nicotinic acid, the ATP-dependent synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O + nicotinate = ADP + diphosphate + nicotinate beta-D-ribonucleotide + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <comment type="PTM">
        <text evidence="8">Transiently phosphorylated on a His residue during the reaction cycle. Phosphorylation strongly increases the affinity for substrates and increases the rate of nicotinate D-ribonucleotide production. Dephosphorylation regenerates the low-affinity form of the enzyme, leading to product release.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the NAPRTase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="4 8"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122512.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13767"/>
    <dbReference type="EnsemblBacteria" id="EHS85581">
        <property type="protein sequence ID" value="EHS85581"/>
        <property type="gene ID" value="PS3_13767"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1400"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041525">
        <property type="entry name" value="N/Namide_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041619">
        <property type="entry name" value="NAPRTase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040727">
        <property type="entry name" value="NAPRTase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006405">
        <property type="entry name" value="Nic_PRibTrfase_pncB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098">
        <property type="entry name" value="PTHR11098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04095">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17956">
        <property type="entry name" value="NAPRTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17767">
        <property type="entry name" value="NAPRTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="SSF51690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01513">
        <property type="entry name" value="NAPRTase_put"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 8" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NAPRTase_N" evidence="10">
        <location>
            <begin position="12"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="NAPRTase" evidence="9">
        <location>
            <begin position="157"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="NAPRTase_C" evidence="11">
        <location>
            <begin position="363"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS85581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="488" mass="55668" checksum="70717890979BD683" modified="2012-04-18" version="1">MEYLYPDDSYTLHTDAYELTMMQTYWQEGIADRKAVFEVFFREMPFDNGYAIYAGLDHLIQYIQNLHFSTTDIDYLRTTAMFDDQFLDYLATFKFQGNVRSMVEGEIVFNHEPLVQIEGNLLECQLVETAILNIINFQIMVATKAARIKSVVGNQAVMEFGSRRAQEFDAALWGTRAAYVGGFDATSNVRAGKLFGIPISGTHAHSLVQTYRNDYDAFKAYAQTHRDCVFLVDTYDTLKSGVPSAIKIADEFGDQINFIGIRIDSGDMAYLSKQARKMLDEAGYPNAKIFASNDLDETTIQNLLMQGAKIDTWGVGTKLITSYDQPTLGAVYKMVSIEDDQGQMVDTIKLSNNTSKMSTPGKHQVWRITREADGKSEGDYVTLVHEDPRDQESLYMFDPNNPFLNKTVHGFIAEPLLHDIFINGELVYDEPSLDEIRALRADHLEALWDEYKRDLNPQIYPVDLSQECYDHKLLTIREVHDYVNQLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKD2</accession>
    <name>H4GKD2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_11360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122515.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11360"/>
    <dbReference type="EnsemblBacteria" id="EHS85584">
        <property type="protein sequence ID" value="EHS85584"/>
        <property type="gene ID" value="PS3_11360"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1403"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="212" mass="24989" checksum="AB123A6725AD0F82" modified="2012-04-18" version="1">MPKQGQFARQSRQSKLKATKQKLKRQKQTITDQQMSDFLVVRYALTLRQRTAANSQETLQRFIQEIADHLRQTQGDFERLLPMIWLKVRGQVPWQFYWQLSLNWPQLAPFLHREINALPLAQPIWAQQLPTPEAFDAQLLNELVNQIIATTTLNHPLKSEQKEQLKQRLTATLWIDNQIQWEQIRQLLGPFQQTITSNDSGSQVWLDRLARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKF7</accession>
    <name>H4GKF7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative MDR transporter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_9926</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the major facilitator superfamily. EmrB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000065">
        <property type="protein sequence ID" value="EHS85431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122539.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000065.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS85431">
        <property type="protein sequence ID" value="EHS85431"/>
        <property type="gene ID" value="PS3_9926"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1473"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="221"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="557"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="9">
        <location>
            <begin position="9"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="586" mass="62775" checksum="B4B9A3102F9820DF" modified="2012-04-18" version="1">MTKATKIISFIGLTIAILMGTLDSTIVNIALPQIMTHFDASLTDTSWVATIYTLALAVFMITGSKIADRYGRKKIMLLGLALFGGFSAACMLATSLPELIAFRFFQGLGGAIITPIVLPMGIEIFGKAHMSKIASVVGAVTALAAAGGPPIGGVIIKYASWRWVFGINVPLTIAAFLIIAMGVRESYDESLAGRIDLPGTILLTLGLGGLTFGLLEGRQYGWQSGIILGTLIGGVLAIGLFIFIESKVKHPLLELDLFREKTLTASSLVYFLTGFTLVSPTLILNYFLQDVLNDTALHAALIIVPVSLTIMVAMPLGTKLFDQVGAVPVTLIGLLVMAASLLLLSFITTTTPKAVIITFLVIHGVGFGFSSVSLVASVKHLPKNKSGIGSGIVNAARQIGTCLGIAILVTVLDNNVNTAKQHIHQDAIQIVDRQQLSPKVNKVVHQQLNSLFSTTESAKLTTKNKQTNLRRALKQAAKSQVDLPQPQKGTDLYQVYQAQKKLALATDNLKAGNQQLQKISQGQKKLLVGLKQLAQKEALTIALRKIKHTKNQQISRAFSHTYLVGAVIVLILSPIALLTDRKSLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKF9</accession>
    <name>H4GKF9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_12193</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000066">
        <property type="protein sequence ID" value="EHS85394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122541.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000066.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12193"/>
    <dbReference type="EnsemblBacteria" id="EHS85394">
        <property type="protein sequence ID" value="EHS85394"/>
        <property type="gene ID" value="PS3_12193"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1478"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:cellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="PTHR31851"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS85394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="230" mass="24610" checksum="A7B1D0DC5C88B53E" modified="2012-04-18" version="1">MQIKTIKHQHQTMEEKLNTLRAGVLGSNDGILTVVGVLVSVAAATSNSFTIFIAGLSDLLACAFSMAVGEYASVSTQKDTEKSVVAKEQHLLKTDFQGELEAVQAYYHSKGLTSDLSLKIAEDLMKKKPLATVLKVKYNIELGHYLDPWAAAFSSLFAAALGGILPLMAMTFVNGFYKWPMTIAAVAASSALTALISSKLGDGDTKVAIIRNIVIGLITIAIHYGVGRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKG4</accession>
    <name>H4GKG4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative serine 3-dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_14841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000067">
        <property type="protein sequence ID" value="EHS85378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14841"/>
    <dbReference type="EnsemblBacteria" id="EHS85378">
        <property type="protein sequence ID" value="EHS85378"/>
        <property type="gene ID" value="PS3_14841"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1488"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="217" mass="23555" checksum="30DE60FAFE928D0C" modified="2012-04-18" version="1">MVMLGARREDRLYKIADEINVKGGRADFRTVDVTNPADVQALVDAAKDSFGGLDVIFNNAGIMPNSPMSAVRTDEWNQMIDVNIKGVLNGIAAVMPTFTAQKHGHIITTSSVAGLKNYVGSGVYGATKFAVKNAMEVTRMESANEGTNIRTTTLYPAAINTELLDHIGDEKTATNMKNFYKQHGISPNAIARVVNFAIDQPEDVDIAEFTIYPTNQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKI7</accession>
    <name>H4GKI7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Competence protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122569.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13029"/>
    <dbReference type="EnsemblBacteria" id="EHS85267">
        <property type="protein sequence ID" value="EHS85267"/>
        <property type="gene ID" value="PS3_13029"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1531"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pribosyltran" evidence="1">
        <location>
            <begin position="178"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="223" mass="25149" checksum="292190D2671AB9D2" modified="2012-04-18" version="1">MKKCLLCHQDIQYQVPLSQLLFGPVPKEPSLCPDCQKRFRPRTQNGCQGCGRPCDQGLCNDCQLWQNEYGWVLKNHSLYHYDRAMKDFMCQYKFMGDYRLHQVFQEGITQKIQSLQPTLVVPIPATPHTLMTRGFNQAAALYNGPLCELLGHLQADKQPQSSKGRQARLATPQPFVLMCSASLASDRIVLLDDIYTTGRTLYHAAALLRQAGCQDVQSVTLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKJ4</accession>
    <name>H4GKJ4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Methionine aminopeptidase</fullName>
            <shortName evidence="2">MAP</shortName>
            <shortName evidence="2">MetAP</shortName>
            <ecNumber evidence="2 3">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">map</name>
        <name evidence="5" type="ORF">PS3_18174</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2 3">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="2 3"/>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122576.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18174"/>
    <dbReference type="EnsemblBacteria" id="EHS85274">
        <property type="protein sequence ID" value="EHS85274"/>
        <property type="gene ID" value="PS3_18174"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1538"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070084">
        <property type="term" value="P:protein initiator methionine removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01086">
        <property type="entry name" value="MetAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01974">
        <property type="entry name" value="MetAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002467">
        <property type="entry name" value="Pept_M24A_MAP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00500">
        <property type="entry name" value="met_pdase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00680">
        <property type="entry name" value="MAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M24" evidence="4">
        <location>
            <begin position="12"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="285" mass="31375" checksum="251617B3B513389A" modified="2012-04-18" version="1">MISLKSPREIHDMEASGAVLAGMHTGMREIIKPGISSWVIEEFAREYFQDRGAVAAQIGFEGYEYATCVSVNDEICHGFPRKELVLQEGDLVKVDTVVEYDGAFSDSCWSYAVGQVSPQIQDLMKVTKESLYLGIDAAQVGKRLGDIGAVIQHFTEDEHGYGDVRDFVGHGIGPTMHEDPMVAHYGIAGKGKRLQNGMTITIEPMINTGTWEADTSDPSGWVARTADGGWSCQYEHTIAITNDGPKILTSQDPQADAKYLLADYEELFTPHAEAAKRIAQHYQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKL1</accession>
    <name>H4GKL1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bile acid:Na+ symporter family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_13391</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122593.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13391"/>
    <dbReference type="EnsemblBacteria" id="EHS85180">
        <property type="protein sequence ID" value="EHS85180"/>
        <property type="gene ID" value="PS3_13391"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1568"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002657">
        <property type="entry name" value="BilAc:Na_symport/Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004710">
        <property type="entry name" value="Bilac:Na_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361">
        <property type="entry name" value="PTHR10361"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01758">
        <property type="entry name" value="SBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="218"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="319" mass="34466" checksum="2C65FF7210B78444" modified="2012-04-18" version="1">MNKVVAFGKWLSKWFTLFVVVWAVFNYFLPSVSAPLVPNTKYLLGIILFGMGLTLTGKDFERIVKRPIPVIMGTIAHYIIMPGLAWLLCLVFQLKGATAAGVILVGSCPSGTSSSVMAYLAGGDVALDVSIEALSTLLAPLALPSILYFLAGRYVTVPAQSLFTSTLEIVVIPIILGVLVHTFFGKKLRPILEFLPIVSQLAILLIIGAVVSANHANIFTAATAIVIPVVMLHNLLGYSLGYLFSLVLRLRQPQQKALTFEVGMQDSSLGATLALAYFSPAAAIPSTIFSIWHNISGSVLSSWWKNHTKESVEEMLETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKL4</accession>
    <name>H4GKL4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">YbbR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_12063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122596.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12063"/>
    <dbReference type="EnsemblBacteria" id="EHS85183">
        <property type="protein sequence ID" value="EHS85183"/>
        <property type="gene ID" value="PS3_12063"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1571"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012505">
        <property type="entry name" value="YbbR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07949">
        <property type="entry name" value="YbbR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="283" mass="29670" checksum="F3520FE849BA29A0" modified="2012-04-18" version="1">MKNNQKSLWTRPLFLRIVSLVLAIVLFLYVNGGSDGFLRQTTRGNNDSSTSILNSSKSTTVKIPLTVNANTDKYVVTGYPQFVKVKLTGPSALVTTSVNTQNFKAYINLSDLTAGEHTVKVKTSGLNSNVKATVNPAKITVNIEPRSTITASVKVQLSNTSLTGDYKLGNAKPALDTVQVTGARSEVQQVSQVIAYVKVPKNTTATLHQQVTLQAVDKNGKALNVVVTPGTISVTVPVSADDQSSSSQSSDSDNTSSSSSAVSTSESSSSASSSSASSSSSDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKN8</accession>
    <name>H4GKN8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Chaperone protein DnaK</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">HSP70</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Heat shock 70 kDa protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Heat shock protein 70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dnaK</name>
        <name evidence="13" type="ORF">PS3_10981</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Acts as a chaperone.</text>
    </comment>
    <comment type="induction">
        <text evidence="9">By stress conditions e.g. heat shock.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122620.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10981"/>
    <dbReference type="EnsemblBacteria" id="EHS84958">
        <property type="protein sequence ID" value="EHS84958"/>
        <property type="gene ID" value="PS3_10981"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1598"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="PTHR19375"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="SSF100920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="SSF100934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="530"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="579"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="579"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="600"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphothreonine; by autocatalysis" evidence="9">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS84958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="616" mass="66820" checksum="FA62400F763BE2BC" modified="2012-04-18" version="1">MASNKIIGIDLGTTNSAVAVMEGSEPKIITNPEGGRTTPSVVSFKNGEIQVGEVAKRQAITNPNTIKSIKSHMGEEGYTVEIEGKEYTPQQVSAMILQYIKKYAEDYIGDTVSEAVITVPAYFNDAQRQATKDAGKIAGLDVKRIINEPTASSLAYGLDKTDRDEKILVYDLGGGTFDVSILELGDGVFEVLSTNGDTHLGGDDFDQKIIDWLVDEFKAENGIDLSQDKMALQRLKDAAEKAKKDVSGVQEAQISLPFITSGDNGPLHLETTLTRAKFNQLTNDLVERTKQPVLNAMKDAGLSFSDIDEVILNGGSTRIPAVQEMVKELTGKEPNHSINPDEAVALGAAIQGGVLTGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSKSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGNFQLTDIPPAPRGVPQIKVTFDIDRNGIVNVSAEDQGTHKKQNITIKSNSGLTDEEIEQMKKDAEANAKADKKRKEEADLRNEVDQLIFQTDKVLDEMKDKVSEDDLKKAQDAKDALKKAQEDNDLDAMKEKKDDLNKIVQDLSVKLYQQAQDANQAAGGDAQGTDDNKSNDGNTVDGDFEEVDPDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKS1</accession>
    <name>H4GKS1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">1-acyl-sn-glycerol-3-phosphate acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_12879</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122653.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12879"/>
    <dbReference type="EnsemblBacteria" id="EHS84991">
        <property type="protein sequence ID" value="EHS84991"/>
        <property type="gene ID" value="PS3_12879"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1647"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PlsC" evidence="1">
        <location>
            <begin position="34"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="207" mass="23849" checksum="493C40BD6A43BD0C" modified="2012-04-18" version="1">MLYRLVYYIFAPLLALINLRQKVYNKENLPQGNYVLVAPHRTWMDPPLLALAAYPKQFGFMAKQELFKNPVGSWIIRSLHAYPVDRKNPGMSAIKGPVKMLKDTELSTIIFPTGSRYSKKMKGGALLIAKMANVPVVPAVYQGPTNLKELFSWRNRRQVAFGKPIYIDRKTKLTDEVQADLETQIQTQFDQLDHQINPNYHYQIPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKS4</accession>
    <name>H4GKS4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">LexA repressor</fullName>
            <ecNumber evidence="12">3.4.21.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">lexA</name>
        <name evidence="16" type="ORF">PS3_6482</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Hydrolysis of Ala-|-Gly bond in repressor LexA.</text>
            <dbReference type="EC" id="3.4.21.88"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12 13">Belongs to the peptidase S24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.88" evidence="12"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122656.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6482"/>
    <dbReference type="MEROPS" id="S24.001"/>
    <dbReference type="EnsemblBacteria" id="EHS84994">
        <property type="protein sequence ID" value="EHS84994"/>
        <property type="gene ID" value="PS3_6482"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1650"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06529">
        <property type="entry name" value="S24_LexA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00015">
        <property type="entry name" value="LexA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006200">
        <property type="entry name" value="LexA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039418">
        <property type="entry name" value="LexA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006199">
        <property type="entry name" value="LexA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006197">
        <property type="entry name" value="Peptidase_S24_LexA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015927">
        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01726">
        <property type="entry name" value="LexA_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00726">
        <property type="entry name" value="LEXASERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00498">
        <property type="entry name" value="lexA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 13" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="4 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 12 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 12" id="KW-0678">Repressor</keyword>
    <keyword evidence="11 12" id="KW-0742">SOS response</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 12" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="LexA_DNA_bind" evidence="15">
        <location>
            <begin position="8"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S24" evidence="14">
        <location>
            <begin position="87"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="12">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="active site" description="For autocatalytic cleavage activity" evidence="12">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="active site" description="For autocatalytic cleavage activity" evidence="12">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="12">
        <location>
            <begin position="94"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EHS84994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="208" mass="22997" checksum="442A0448F933C37A" modified="2012-04-18" version="1">MPRQSIDKQMAVLSYINKQVDAHGYPPTVREICAAVGLSSTSTVHGHINRLIEKGYLHKDPAKPRALEITQQGLNALGITPEQTEIPLLGEVAAGEPILAEQDATDFFPIPPSIQDHNDLFMLTIKGTSMIKMGILNGDKVIVRRQSTANNGDVVIAMTAENEATCKRFYKEDGYYRLQPENDDLEPIILDQVVILGKVVGLFRDNIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKT4</accession>
    <name>H4GKT4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Hemolysin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PS3_12655</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0053 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12655"/>
    <dbReference type="EnsemblBacteria" id="EHS85004">
        <property type="protein sequence ID" value="EHS85004"/>
        <property type="gene ID" value="PS3_12655"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1662"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="DUF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="13">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="208"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="12">
        <location>
            <begin position="273"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS85004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="448" mass="51421" checksum="B4D0E96E7E7E9575" modified="2012-04-18" version="1">MIIIFILAIAALFTLTEYSLVKVRPTELKELPQTRKVKSALKMMDHLTEYLSTAQVGITITSLVLGWIGEGYITAWLEKLNIFPKNVSEGVSSVIGILIFTFLHAVFTDLVPKNIAIDQPVKVLLFIVNMTRFFHVIFYPLIWIFDRVAIMVTKLLGFNAHPDEDIYSENEIISLSKESEKAGELDREDVVFMQRAFKMNDKVASDIMVDRTQLTVIDITDTIADAAQIYFEKKFTRMPVVANHDKDHILGYIFSYDIMRQNQINPQQTIRVILRRMPIAYESQPITDVLQLMMQRQVPIVIVQDEYGGTSGIITDKDIYEELFGTVGEEIDHISDSMIEQSETDSQGNPTYLVSGKMPLDDFMRYFEVQIPQFETVQVTTLTGFFLEQQYDMKVGQPVRVEDFSFTPTDIENQYVNEFKVTHIKPKKQESENDGNEHSSDNDDEVNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKT5</accession>
    <name>H4GKT5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Lipolytic protein G-D-S-L family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_10553</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122667.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10553"/>
    <dbReference type="EnsemblBacteria" id="EHS85005">
        <property type="protein sequence ID" value="EHS85005"/>
        <property type="gene ID" value="PS3_10553"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1663"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS85005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="377" mass="42683" checksum="70926C1CBE6316BC" modified="2012-04-18" version="1">MQWQSGYYQLTHDLRAMAFQYPELNQVIKFTSFLSGSKLRLTFTNFYGSQTLKFDQILIADNDNFDHAVQVTINHQSELMIPVGQAKVSDPVDFTVQAGRAVYIMVIANQAQTYCDFAVTYDPTWVNASMGRHANHQPPLNDRWQHGHGWVSLSMMEVWTQAQPQVIEFTGDSLVETGMVAAELFKKTIVEKPEQITWLVTGISGNRLLTDAPQNKNPERTYGLSLLHRYGQLGHRPYEPSLTLVSIGANDLLVPFDNPLGMEQIPSLDRLKIGYRDLESIIESRQSEVKILTLPPVRLPRKTGPWTTDEIQIDRVRKSLNVWLRSNSWVIDACGSITDEKGNRKPAVDFGDNLHLSPYGGQLMGQAIWLQLKLALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKV4</accession>
    <name>H4GKV4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Imidazoleglycerol-phosphate dehydratase</fullName>
            <shortName evidence="5">IGPD</shortName>
            <ecNumber evidence="5 6">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hisB</name>
        <name evidence="7" type="ORF">PS3_904</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5 6">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="5 6"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122686.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_904"/>
    <dbReference type="EnsemblBacteria" id="EHS85024">
        <property type="protein sequence ID" value="EHS85024"/>
        <property type="gene ID" value="PS3_904"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1686"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="PTHR23133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00954">
        <property type="entry name" value="IGP_DEHYDRATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 6" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="4 5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="194" mass="21606" checksum="6F9972F351F1821D" modified="2012-04-18" version="1">MRTATITRQTGETKITVELNLDQQTGVEIQTGIGYFDHMLNLFAKHGRFGLKVTAQGDLEVDAHHTVEDTGIVLGECFKQALGDKAQIERYGDAWVPMDEVLAQVVVDLSGRSYLVFDSEFENPRLGDYETEVTEDFFQAVAFAAEMNLHARALYGRNTHHKIEALFKAFARAMRKAVTINPEIVGVNSTKGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKX7</accession>
    <name>H4GKX7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">dTDP-glucose 4,6-dehydratase</fullName>
            <ecNumber evidence="4 5">4.2.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_22152</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H2O</text>
            <dbReference type="Rhea" id="RHEA:17221"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57477"/>
            <dbReference type="ChEBI" id="CHEBI:57649"/>
            <dbReference type="EC" id="4.2.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 5">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.46" evidence="4 5"/>
    <dbReference type="EMBL" id="AICN01000075">
        <property type="protein sequence ID" value="EHS84841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122709.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000075.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_22152"/>
    <dbReference type="EnsemblBacteria" id="EHS84841">
        <property type="protein sequence ID" value="EHS84841"/>
        <property type="gene ID" value="PS3_22152"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1717"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05246">
        <property type="entry name" value="dTDP_GD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005888">
        <property type="entry name" value="dTDP_Gluc_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01181">
        <property type="entry name" value="dTDP_gluc_dehyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="7">
        <location>
            <begin position="6"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="132"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="343" mass="38665" checksum="6408C6EE80796112" modified="2012-04-18" version="1">MFKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKANLAGLPEDRVKLVVGDICDKELVDQLVKDADAVVHYAAESHNDNSLRDPEPFIQTNIVGTSVLINACRKYNVRFHHISTDEVYGDLPLREDLPGHGEGPGEKFTPESPYRPSSPYSSSKASSDLLVRAWVRSFGLKATISNCSNNYGPYQHIEKFIPRQITNILSGDRPKLYGTGKNVRDWIHTNDHSRAVWQILTEGKIGETYLIGADGEKNNKEVLELILELMGQPKDAYDQVNDRPGHDLRYAIDATKLRTELGWEPIYTDFKKGLQETIDWYTDHQDWWRDEKAAVEAKYAQNGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL03</accession>
    <name>H4GL03_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptide methionine sulfoxide reductase MsrA</fullName>
            <shortName evidence="4">Protein-methionine-S-oxide reductase</shortName>
            <ecNumber evidence="4">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Peptide-methionine (S)-S-oxide reductase</fullName>
            <shortName evidence="4">Peptide Met(O) reductase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">msrA</name>
        <name evidence="6" type="ORF">PS3_19766</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>[thioredoxin]-disulfide + H2O + L-methionine = [thioredoxin]-dithiol + L-methionine (S)-S-oxide</text>
            <dbReference type="Rhea" id="RHEA:19993"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58772"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 4">
            <text>[thioredoxin]-disulfide + H2O + L-methionyl-[protein] = [thioredoxin]-dithiol + L-methionyl-(S)-S-oxide-[protein]</text>
            <dbReference type="Rhea" id="RHEA:14217"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12315"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:44120"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122735.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19766"/>
    <dbReference type="EnsemblBacteria" id="EHS84769">
        <property type="protein sequence ID" value="EHS84769"/>
        <property type="gene ID" value="PS3_19766"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1751"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033744">
        <property type="term" value="F:L-methionine:thioredoxin-disulfide S-oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="SSF55068"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PMSR" evidence="5">
        <location>
            <begin position="5"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS84769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="175" mass="20159" checksum="135078F36E2841E2" modified="2012-04-18" version="1">MSSETAIFAGGCFWCMVKPFDTYPGIQKVEAGYTGGHVQDPTYEQVKHGGTGHTEAVRITFDPEVVSYDQLVDLYWQQTDPTDAFGQFQDRGDNYRPVIFVKDAYQDQVARASRTRLAQSGRFTEPIVTQIEPAQPFYLAEDYHQAFYKKNPARWREEEGARQEFISQHWNNSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL40</accession>
    <name>H4GL40_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 11">Trigger factor</fullName>
            <shortName evidence="9">TF</shortName>
            <ecNumber evidence="9">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">PPIase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tig</name>
        <name evidence="14" type="ORF">PS3_8098</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9 10">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="9">About half TF is bound to the ribosome near the polypeptide exit tunnel while the other half is free in the cytoplasm.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">Consists of 3 domains; the N-terminus binds the ribosome, the middle domain has PPIase activity, while the C-terminus has intrinsic chaperone activity on its own.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 11">Belongs to the FKBP-type PPIase family. Tig subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122772.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8098"/>
    <dbReference type="EnsemblBacteria" id="EHS84670">
        <property type="protein sequence ID" value="EHS84670"/>
        <property type="gene ID" value="PS3_8098"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1795"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00303">
        <property type="entry name" value="Trigger_factor_Tig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005215">
        <property type="entry name" value="Trig_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008880">
        <property type="entry name" value="Trigger_fac_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037041">
        <property type="entry name" value="Trigger_fac_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008881">
        <property type="entry name" value="Trigger_fac_ribosome-bd_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036611">
        <property type="entry name" value="Trigger_fac_ribosome-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30560">
        <property type="entry name" value="PTHR30560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05698">
        <property type="entry name" value="Trigger_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05697">
        <property type="entry name" value="Trigger_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003095">
        <property type="entry name" value="Trigger_factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102735">
        <property type="entry name" value="SSF102735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="SSF109998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00115">
        <property type="entry name" value="tig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 9 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="5 9 11" id="KW-0143">Chaperone</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 10" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="13">
        <location>
            <begin position="164"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="256"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS84670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="436" mass="48750" checksum="57776F42E80A886C" modified="2012-04-18" version="1">MSVKWERESEASQGTLTFDIDVDTIKKGIDQAFKQTQKRIAVPGFRKGHVPRTIFNQMYGEESLYQDALNAVLPDAYDAAVKEASIEPVDQPEIDIVSMEKGQPWTLTAKVAVKPEVKLGDYKDLEVTRQNTRVSNAEVDEDLERKRQQQAELVLKEDGAAEKGDTVVIDYVGTVDGEEFDGGSADNYSLELGSNSFIPGFEDQLVGHKTDEVVDVNVTFPDEYQAKELEGKDALFKVTIHEIKEKQLPELDDEFAKDVDEDVDTLDELKEKTKENIKEQKQAAAKAAIEDEAIQKAVENAEFEAIPQAMLDDDTNRQVQQYLAGMQQQGINPQMYFQITNTTEDDLKDQFSADAESRVKTNLLLEAVVKDANLTASEEEIAQEVKDLAEQYGMEESAIKQALSDDMLAHDIEVKKAVDLIADSAKQTLKKDEEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL64</accession>
    <name>H4GL64_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA-specific 2-thiouridylase MnmA</fullName>
            <ecNumber evidence="7">2.8.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mnmA</name>
        <name evidence="8" type="ORF">PS3_4602</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47032"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11726"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="Rhea" id="RHEA-COMP:11728"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:87170"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MnmA/TRMU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.13" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122796.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4602"/>
    <dbReference type="EnsemblBacteria" id="EHS84694">
        <property type="protein sequence ID" value="EHS84694"/>
        <property type="gene ID" value="PS3_4602"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1824"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01998">
        <property type="entry name" value="tRNA_Me_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00144">
        <property type="entry name" value="tRNA_thiouridyl_MnmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023382">
        <property type="entry name" value="Adenine_a_hdrlase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004506">
        <property type="entry name" value="tRNA-specific_2-thiouridylase"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00420">
        <property type="entry name" value="trmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 7" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Interaction with target base in tRNA" evidence="7">
        <location>
            <begin position="98"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with tRNA" evidence="7">
        <location>
            <begin position="150"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with tRNA" evidence="7">
        <location>
            <begin position="314"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="7">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="12"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with tRNA" evidence="7">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with tRNA" evidence="7">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Alternate" evidence="7">
        <location>
            <begin position="103"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="377" mass="42378" checksum="7C132DC7F3ADC9E2" modified="2012-04-18" version="1">MTDNSQTRVVVGMSGGVDSSVTALLLKRQGYDVVGVFMKNWDDTDENGVCTATEDFKDVAKVATQIGIPYYSVNFEKEYWDRVFKYFLAEYKKGRTPNPDVVCNKEIKFKAFIEYANQLGADYIATGHYADLKRDADGKMHLMQAKDQNKDQTYFLSQLDHAQLDRVMFPLANYTKPEIRQIAKEAGLVNAKKKDSVGICFIGEDGNFRNFLSQYLPAQPGEMQTTDGKVVGQHAGLMYYTIGQRKGLGLGGTKENNDPWFVVGKDQAQNILYVGQGYDNENLIATHLEASDIHWVDDVPATYGTTFHCTAKFRYRQKDVGVTVKLSDDQSQVTVEFDEPARSITPGQAVVFYDGQECLGSAIIDRAYHEQKLLQYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL70</accession>
    <name>H4GL70_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_20614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122802.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20614"/>
    <dbReference type="EnsemblBacteria" id="EHS84700">
        <property type="protein sequence ID" value="EHS84700"/>
        <property type="gene ID" value="PS3_20614"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1832"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040591">
        <property type="entry name" value="YlmH_RBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17774">
        <property type="entry name" value="YlmH_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="1">
        <location>
            <begin position="184"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="261" mass="30179" checksum="EC716F84FDBD493B" modified="2012-04-18" version="1">MDNLNVKQHFRPDEGPLIDQIQDWLETVRDQYRPILTWFLNPRQRYILTTMVNQDDQIKVAFNGGFANAEMQRALIFPDYYQPQDSDFELQLLTIDYPRKFAELHHRQIMGSLLSSGIDRNVFGDILVHDQDWQVIVDTKMVTYIQGQVTQVGKIHISFLPQAMSAVIQSQSDWEELSTTVVSLRLDAIVATAFNYSRNRAKLVIEHGLVRVNWQEVSRPDFTVAIFDQLSVRHAGRVQLQAVNGYNKKQKIRISLGIIHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL77</accession>
    <name>H4GL77_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phospho-N-acetylmuramoyl-pentapeptide-transferase</fullName>
            <ecNumber evidence="8">2.7.8.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">UDP-MurNAc-pentapeptide phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mraY</name>
        <name evidence="9" type="ORF">PS3_4601</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the initial step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan: transfers peptidoglycan precursor phospho-MurNAc-pentapeptide from UDP-MurNAc-pentapeptide onto the lipid carrier undecaprenyl phosphate, yielding undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide, known as lipid I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>di-trans,octa-cis-undecaprenyl phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine = Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + UMP</text>
            <dbReference type="Rhea" id="RHEA:21920"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:60032"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="ChEBI" id="CHEBI:70758"/>
            <dbReference type="EC" id="2.7.8.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyltransferase 4 family. MraY subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.13" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122809.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4601"/>
    <dbReference type="EnsemblBacteria" id="EHS84707">
        <property type="protein sequence ID" value="EHS84707"/>
        <property type="gene ID" value="PS3_4601"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1840"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008963">
        <property type="term" value="F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051992">
        <property type="term" value="F:UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine:undecaprenyl-phosphate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06852">
        <property type="entry name" value="GT_MraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00038">
        <property type="entry name" value="MraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003524">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018480">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PTHR22926"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10555">
        <property type="entry name" value="MraY_sig1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00445">
        <property type="entry name" value="mraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01347">
        <property type="entry name" value="MRAY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01348">
        <property type="entry name" value="MRAY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0133">Cell shape</keyword>
    <keyword evidence="7 8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS84707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="311" mass="34864" checksum="BC96F7C33A7D0634" modified="2012-04-18" version="1">MSFWFLGLSLICALALTWLAMPTLIRYFIAKKEGQQIREEGPTWHAKKSGTPTMGGLMFVLAATFSTLLTGWLAGQLTAMLWAVLLVWIVYSLIGMWDDSIKIFRQQNEGFKPWQKFLCQVIAAIIFVAIFRSDDPSVGWLYMAFIVFWLVGFSNAVNLTDGLDGLVTGLATISFSAYLILALFQHQYDVAIFCTAVIGSLIGFFPFNHKPAKIFMGDMGSLALGGALAAVAIMVHHPWSLLLIGLIYVIETASVILQVFWFRRTGKRIFKMTPIHHAFEMDGWSEWKIDYVFWLVQLVAAAISVIVLMAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL86</accession>
    <name>H4GL86_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD kinase</fullName>
            <ecNumber evidence="5">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nadK</name>
        <name evidence="6" type="ORF">PS3_10202</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + NAD(+) = ADP + H(+) + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NAD kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122818.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10202"/>
    <dbReference type="EnsemblBacteria" id="EHS84716">
        <property type="protein sequence ID" value="EHS84716"/>
        <property type="gene ID" value="PS3_10202"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1852"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="45"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="121"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="160"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS84716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="269" mass="30578" checksum="8BCA69C4C8FD14D5" modified="2012-04-18" version="1">MKYAIYTYDSEQSLQVRQEILDQAEGTELEYDETYPEVVISVGGDGTLLSAIHKYQDQLDQIRFVGIHTGHLGFYADWRPYEVAELVESLKNDSGQSVTYPLLKTEALYDDGSIEESVAINEATIRDFSKTLVCDVYINHNLFERFRGDGLCISTPTGSTAYNKSVGGAIMDPRIIGFQVAEMASLNNMIFRTIGSPVILGTHAQLNIKIMNDENLILTNDREITKLDDDQRHLTEIKYCVASQRVYFAKYRHTSFWKRVRDSFIGDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHQ3</accession>
    <name>H4GHQ3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Accessory Sec system protein translocase subunit SecY2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">secY2</name>
        <name evidence="3" type="ORF">PS3_10173</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Part of the accessory SecA2/SecY2 system specifically required for export of possible cell wall proteins. The central subunit of a protein translocation channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the accessory SecA2/SecY2 protein translocase complex required to export cell wall proteins. May form heterotrimers with SecE and SecG subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecY/SEC61-alpha family. SecY2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000002">
        <property type="protein sequence ID" value="EHS87558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10173"/>
    <dbReference type="EnsemblBacteria" id="EHS87558">
        <property type="protein sequence ID" value="EHS87558"/>
        <property type="gene ID" value="PS3_10173"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.2"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01466">
        <property type="entry name" value="SecY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014269">
        <property type="entry name" value="SecY2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="PTHR10906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="SSF103491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02920">
        <property type="entry name" value="acc_sec_Y2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="276"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="335"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="401" mass="44910" checksum="29013BE020D40FE4" modified="2012-04-18" version="1">MNKHPLIERMLWSCWILLIYIFGQEIPLPLVKYQSHIRSVVSEPIRAVISGSGMSNYSIFALGIGPMMIGLIVAAFAMQSKVLGLNRLPSKYNDRIQMIITILIALAQAYVSVRNFKLTKPGWETEWQVILVMVAGAMMIMWLCNMNAKLGLGGPMMIIMANVMVSLLRTVYNSRRLITGGLGTWLIVIIGLVILVVVIISITLLMDRAEYRIPVIRLGINNQFVDKSYLPVKLSPAGGMPVMFAMAILSLPQYLFHYLAQAYPHNATFAWWNDNLVLSKTAGVTTYIIILFLFSIAFALMNMDPEQLAEDYQKSGDYIPGFRPGRVTARYLRGVIVRFSIIGGIFITLIAGLPLIFAIDRPQYETLMMFPGNVMMAASFAILMLDQIDVILVHGKYDSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHQ6</accession>
    <name>H4GHQ6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_15234</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000002">
        <property type="protein sequence ID" value="EHS87561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121590.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000002.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87561">
        <property type="protein sequence ID" value="EHS87561"/>
        <property type="gene ID" value="PS3_15234"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.14"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="338" mass="39324" checksum="0571EDC1D48C167B" modified="2012-04-18" version="1">MSNLNHVSVKGFYGQLKTLLKNKQYWSAVWPAALFTIIFGLLYIVTAGIGLSFLGTVFTFITQYMMYIMYGLISPIYILMYLIPSIVGFVAAFFVLYWYTFILVSFMYSYQDVLREESDHVTVATIWKHFRHLRKNQILRISLYQQLFTFLWILPFEIINLIWGTNAYISGAMNVLILLVALWKTLQYAQSYFLYREKQPAFVGQSMRHALTGSKRYMYGLKFNYLWIELLYGCLPVAVWSAIFGGLAYYGAYTATDVWLYLGLALVALGILFYLPTLLTIPAVYFETSKKTIKVDTLFEETFTPVEILAGQAFKEDYQAPKSENKKRFKLSFKRKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHQ7</accession>
    <name>H4GHQ7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase IS66</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_9752</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000003">
        <property type="protein sequence ID" value="EHS87552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9752"/>
    <dbReference type="EnsemblBacteria" id="EHS87552">
        <property type="protein sequence ID" value="EHS87552"/>
        <property type="gene ID" value="PS3_9752"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004291">
        <property type="entry name" value="Transposase_IS66_central"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03050">
        <property type="entry name" value="DDE_Tnp_IS66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_IS66" evidence="1">
        <location>
            <begin position="2"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="137" mass="15798" checksum="EA1AFB4ED960E4DF" modified="2012-04-18" version="1" fragment="single">MAKEVLKLMGAVFHTEKNLQYGSAAEKLELRNQLVKPKLDKVYDFLTKIKHPMGKLKRAINNALKLKDRLYKIFDNAQLPLDNNPVEQSIRPTTLVRKNSLFATSQNGAKANAMIYTIIQTVKLNNLRIFDYLKYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHT1</accession>
    <name>H4GHT1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipolytic protein G-D-S-L family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_8063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8063"/>
    <dbReference type="EnsemblBacteria" id="EHS87487">
        <property type="protein sequence ID" value="EHS87487"/>
        <property type="gene ID" value="PS3_8063"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.50"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH_hydro" evidence="1">
        <location>
            <begin position="31"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="287" mass="32565" checksum="276EF43613A3F2F5" modified="2012-04-18" version="1">MIGGGGYYYWQQAASQNRPKKVLKSKIKLVAIGDSLTQGVGDQKQQGGYVGIIKHLLANQYHNQVITYNYGKSGDRSDQILQRLHNQSIIQHNLKSADVIVMTVGGNDLSQVLYAKAPGKSANQVETAVQKAGISYRKKLRQLMRAIRQENKTAPIFVISIYNPYYVYFPNVTTMENSVTIWNHYTQATVQKYQQTYFVNIQKLMSYGQYTSASSRSSLGSQTKQANQSSTKQSDLIQVMNTSSNLNNYISPSDNYHPNYRGYTKMAQALLKVMKQHNQFEYRIVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHT8</accession>
    <name>H4GHT8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TPR repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_15347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121622.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15347"/>
    <dbReference type="EnsemblBacteria" id="EHS87494">
        <property type="protein sequence ID" value="EHS87494"/>
        <property type="gene ID" value="PS3_15347"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.57"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="238"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="422" mass="48222" checksum="061D29B6E665A6D5" modified="2012-04-18" version="1">MSYSEKMLDQLDRGQLAEAKKSFAWALRKDDDETLFNLAEELYGLGFLNQAQRVYLKLLEKYPDEDVLRTALAEIAIDNDHNDEALTYLNQIAADSDAYVPALLVAADLYQTEEQFEVTESKLLEAYQIAPDEPAVLFALGEFYNLIGKPEQAIQYYFALIQAGYLEFAKVDIAGRLGTAYAQSGHFDQALGYLEQVDPQYQSSDIRFQTGFTQLQLGKLKAAEQSLQSLIDDDSQYASAYPALATVYEQENDYQQALKTVQEGLSVDQYNENLYAQAADYASHLGDDPLMDQYLQRAHEIDPDNLTITIQYSNFLLSQQRDQDNLNLLASVEDDEVVDPQVEWNRAQSYWRLEQFDQAGQAFSAALPAFEDNPDFLHQLIDYYQAVGERDLMFDQLQRYVELVPTDTEMAERLAEFEDQMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHW2</accession>
    <name>H4GHW2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Tetrahydrodipicolinate N-acetyltransferase</fullName>
            <shortName evidence="7">THP acetyltransferase</shortName>
            <shortName evidence="7">Tetrahydropicolinate acetylase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dapH</name>
        <name evidence="9" type="ORF">PS3_6134</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the transfer of an acetyl group from acetyl-CoA to tetrahydrodipicolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + acetyl-CoA + H2O = CoA + L-2-acetamido-6-oxoheptanedioate</text>
            <dbReference type="Rhea" id="RHEA:13085"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58117"/>
            <dbReference type="EC" id="2.3.1.89"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (acetylase route): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the transferase hexapeptide repeat family. DapH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.89" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121646.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6134"/>
    <dbReference type="EnsemblBacteria" id="EHS87518">
        <property type="protein sequence ID" value="EHS87518"/>
        <property type="gene ID" value="PS3_6134"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.87"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00022"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047200">
        <property type="term" value="F:tetrahydrodipicolinate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01691">
        <property type="entry name" value="DapH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019873">
        <property type="entry name" value="DapH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013710">
        <property type="entry name" value="DapH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08503">
        <property type="entry name" value="DapH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03532">
        <property type="entry name" value="DapD_Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DapH_N" evidence="8">
        <location>
            <begin position="4"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="237" mass="24977" checksum="5F01A7E1EEA9D5F5" modified="2012-04-18" version="1">MAQLDAQTIINYIGTAPKKTPVKVYVKGEQLDQLTYPDGVDAFVEKRTGTLFGDWQVVKPFLDRYQDNFDSVRIENSARNSAVPLLDLKEINARIEPGAVIRDQVVIGDNAVIMMGATINIGAEIGADSMIDMGAILGGRAIVGKHCHIGAGTVLAGVIEPASAEPVRIDDNVLIGANAVVIEGVHVGEGAVVAAGAVVTEDVAPKTVVAGVPARVIKQVDDQTTSKTGLEDDLRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHW3</accession>
    <name>H4GHW3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Diaminopimelate decarboxylase</fullName>
            <shortName evidence="2">DAP decarboxylase</shortName>
            <shortName evidence="2">DAPDC</shortName>
            <ecNumber evidence="2 3">4.1.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lysA</name>
        <name evidence="6" type="ORF">PS3_6494</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>H(+) + meso-2,6-diaminoheptanedioate = CO2 + L-lysine</text>
            <dbReference type="Rhea" id="RHEA:15101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="4.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 3">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; L-lysine from DL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orn/Lys/Arg decarboxylase class-II family. LysA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.20" evidence="2 3"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121647.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6494"/>
    <dbReference type="EnsemblBacteria" id="EHS87519">
        <property type="protein sequence ID" value="EHS87519"/>
        <property type="gene ID" value="PS3_6494"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.88"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008836">
        <property type="term" value="F:diaminopimelate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06828">
        <property type="entry name" value="PLPDE_III_DapDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02120">
        <property type="entry name" value="LysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002986">
        <property type="entry name" value="DAP_deCOOHase_LysA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01181">
        <property type="entry name" value="DAPDCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01048">
        <property type="entry name" value="lysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn_Arg_deC_N" evidence="5">
        <location>
            <begin position="40"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Orn_DAP_Arg_deC" evidence="4">
        <location>
            <begin position="299"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="291"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="391"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="437" mass="48190" checksum="3F5239A2379C9B6D" modified="2012-04-18" version="1">MNDQIRPDQINSQGHLQIGGVDSLTLAEQYGTPLVVYDVAQIREQIHRFQAVFEANHVQSEVSYASKAFAAIAIYQVVNQEHGHIDVVSGGELMTAIKARFPMDRVTFHGNNKSEAELRLALHHQVGMIVIDNFYEIELLSRLLDELDLQTPVMLRITPGISAHTNEYIQTGQVDSKFGFDLGSGQADQALQAVLSNPRFQMKGIHAHIGSQIFEVAGFEGVANKLVEVAKHWQSEFNYQTEILNVGGGFGIRYVENDHPLAPEEFVDAIVKAVKKAAKVAKIPEPGIWIEPGRSIVGPAGYNLYTVGSRKDIPGYKPFLAVDGGMGDNIRPALYEAEYETVLAKDPAAENVETVRIAGKYCESGDILSRSQALPKTEPGDVLAMLDTGAYGFAMASNYNRNPRPAVVFVENGQSQLVIQHETWEDMARLDLPYELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHW4</accession>
    <name>H4GHW4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Aspartokinase</fullName>
            <ecNumber evidence="12">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PS3_2774</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the phosphorylation of the beta-carboxyl group of aspartic acid with ATP to yield 4-phospho-L-aspartate, which is involved in the branched biosynthetic pathway leading to the biosynthesis of amino acids threonine, isoleucine and methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP + L-aspartate = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the aspartokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="12"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121648.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2774"/>
    <dbReference type="EnsemblBacteria" id="EHS87520">
        <property type="protein sequence ID" value="EHS87520"/>
        <property type="gene ID" value="PS3_2774"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.89"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00015"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00461"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04245">
        <property type="entry name" value="AAK_AKiii-YclM-BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035804">
        <property type="entry name" value="AKIII_YclM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005260">
        <property type="entry name" value="Asp_kin_monofn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042199">
        <property type="entry name" value="AsparK_Bifunc_asparK/hSer_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018042">
        <property type="entry name" value="Aspartate_kinase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000726">
        <property type="entry name" value="Asp_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00324">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="7 12 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="14">
        <location>
            <begin position="391"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="5"/>
            <end position="8"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="220"/>
            <end position="221"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000726-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EHS87520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="457" mass="51273" checksum="CE7151B3A873B308" modified="2012-04-18" version="1">MKVIKFGGSSLANGDAFAQAISIIQADDERRVIVTSAPGKRFDDDIKVTDLLINFAKLTINGHNTQDVIASIFGRYQSIGHHFHVQDNDLQAIHDQLVALPKQHYPNNDYLMAAFKAHGERLNAMLMAIILNHQGIKARFIDPLDAGLRVTGTPNNAVVSPEAYINLSNLQYSDDERLIFPGFYGYSLSGYIATFSRGGSDVTGAILARAFHADMYENFTDVDAIYSANPKFVQDPLPIQRMTYREMRELSYAGFSVFHDEALIPAIQGQIPINVKNTWHPEKPGTFIVPEKNFKPDHIMTGIASGRHFSALYLHKYLLNKENGFTLRILQILDRNGVSYEHMPSGIDDITIIFDRNKLSDEQVDIVCSEIQSEVNPDRLEWIEDYAIIMVVGEGMANRVGVMRDIVVPIAENNISLSMVNQGSSEISIMIGTHRKDAINAVRAIYDYFFNQLEDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHW9</accession>
    <name>H4GHW9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">DNA primase</fullName>
            <ecNumber evidence="12">2.7.7.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">dnaG</name>
        <name evidence="17" type="ORF">PS3_5702</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 13 14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12 13 14">Binds 1 zinc ion per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer. Interacts with DnaB.</text>
    </comment>
    <comment type="domain">
        <text evidence="12">Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">Belongs to the DnaG primase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.101" evidence="12"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5702"/>
    <dbReference type="EnsemblBacteria" id="EHS87525">
        <property type="protein sequence ID" value="EHS87525"/>
        <property type="gene ID" value="PS3_5702"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.95"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03364">
        <property type="entry name" value="TOPRIM_DnaG_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00974">
        <property type="entry name" value="DNA_primase_DnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016136">
        <property type="entry name" value="DNA_helicase_N/primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013264">
        <property type="entry name" value="DNA_primase_core_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037068">
        <property type="entry name" value="DNA_primase_core_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019475">
        <property type="entry name" value="DNA_primase_DnaB-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006295">
        <property type="entry name" value="DNA_primase_DnaG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036977">
        <property type="entry name" value="DNA_primase_Znf_CHC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030846">
        <property type="entry name" value="DnaG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034151">
        <property type="entry name" value="TOPRIM_DnaG_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002694">
        <property type="entry name" value="Znf_CHC2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10410">
        <property type="entry name" value="DnaB_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08275">
        <property type="entry name" value="Toprim_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01807">
        <property type="entry name" value="zf-CHC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002811">
        <property type="entry name" value="DnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00400">
        <property type="entry name" value="ZnF_CHCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01391">
        <property type="entry name" value="dnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12 13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 12 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2 12 13" id="KW-0639">Primosome</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 12 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 12 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="16">
        <location>
            <begin position="263"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CHC2-type" evidence="12 14">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="440"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR002811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR002811-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EHS87525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="596" mass="67950" checksum="C14283230DB1F6E9" modified="2012-04-18" version="1">MARIPNEVIDQVRNSVDIADVIGQDVQLRKQGRNLMGHCPFHQDDTPSFSVNEEKQFFYCFSCHRTGNVFSFLQQLHEMTFPEAVQHVAEMANIEIPELNQRDQSTASPNAPFYQLYDLAADLYHHILVNTRAGQPALKYLEQRGMTRELIDQFKLGFAPSSDDHGILAQFLNEKQVDYQVIRQSGLLTTDHDGHFHDRFVDRVMYPIKNANGQIIAFSGRLLAAKGNDQAPKYLNSPETPIFNKRQTLFNFDEAKRAVRQEGHLILMEGFMDVISAYGAGVKSGIASMGTSFTEEQIQAIQRISNELVVCYDGDDAGQNAIMRALQLLTDQAPTLKLKVVQMPAGLDPDEFVQQRGPEKFQQYLTNVEESPTAFQLQYLRRGLNLANQNELLSYIDTAIRVIAQVEQPVAQNLYLKQLADEFNLDQATLAAQLSQVEQQAQRSRPKVTKPQQEQSTSDFNHRRKTINSTSPALVNRVQRAEQLLIYWFIYQPEWRGHLLGLTDFTFPDDNYQQLFEQIQDYLQAHADYLTGDMINFLQDQPELIALLAEIEQLEVQDEITDEMVDDCVHVLQSEAPIEDLISQKQGGIKRGHRSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHZ6</accession>
    <name>H4GHZ6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carbamoyl-phosphate synthase small chain</fullName>
            <ecNumber evidence="2">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Carbamoyl-phosphate synthetase glutamine chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">carA</name>
        <name evidence="4" type="ORF">PS3_20882</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the CarA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000006">
        <property type="protein sequence ID" value="EHS87475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121680.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000006.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20882"/>
    <dbReference type="EnsemblBacteria" id="EHS87475">
        <property type="protein sequence ID" value="EHS87475"/>
        <property type="gene ID" value="PS3_20882"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.126"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="SSF52021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CPSase_sm_chain" evidence="3">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="CPSase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="331"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="362" mass="39692" checksum="C7C02F44F443385C" modified="2012-04-18" version="1">MQRYLVLEDGTVYAGEAFGADTETVGEVVFTTGMTGYQEAITDQSYANQILVFTNPLIGNYGVNVDDDESLQPQIKGVICNEVARVPSNWRMQGSLPDYLKQIDVPGLKGIDTRELVRKLRYHGTLRGTLVADVDYLDAIIEDLHEAQPTKGIIAKVSTKSPYPNPGLERNIVVVDFGLKHSILQELAKRGCNTTVLPYSATAEEILNLHPDGVLLSNGPGDPQEMTAAAEMVAEVEKHVPIFGICMGHQVFALANGAQTFKMKFGHRGFNHPVRNIATGEIAFTSQNHGYAVDPESVKNTDLMVTYEEINDGIVEGLRHRDYPAFSVQFHPDAAPGPNDADSIFDDFVSLVDQRKGDRTNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI18</accession>
    <name>H4GI18_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA topoisomerase 4 subunit A</fullName>
            <ecNumber evidence="7">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Topoisomerase IV subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">parC</name>
        <name evidence="10" type="ORF">PS3_6491</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotetramer composed of ParC and ParE.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the type II topoisomerase GyrA/ParC subunit family. ParC type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000009">
        <property type="protein sequence ID" value="EHS87447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121702.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000009.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6491"/>
    <dbReference type="EnsemblBacteria" id="EHS87447">
        <property type="protein sequence ID" value="EHS87447"/>
        <property type="gene ID" value="PS3_6491"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.156"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019897">
        <property type="term" value="C:extrinsic component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00937">
        <property type="entry name" value="ParC_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006691">
        <property type="entry name" value="GyrA/parC_rep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035516">
        <property type="entry name" value="Gyrase/topoIV_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005741">
        <property type="entry name" value="TopoIV_A_Gpos"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43493:SF9">
        <property type="entry name" value="PTHR43493:SF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03989">
        <property type="entry name" value="DNA_gyraseA_C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101904">
        <property type="entry name" value="SSF101904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01061">
        <property type="entry name" value="parC_Gpos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="TOP4c" evidence="9">
        <location>
            <begin position="9"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="427"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="7">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="7">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="7">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="7">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="7">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="7">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="831" mass="92923" checksum="3398310F3711F848" modified="2012-04-18" version="1">MPDSKIEVMNLEAIMGDRFGRYSKSIIQERALPDIRDGLKPVQRRILFAMNKDGNTYDKGFRKSAKSVGNVMGNFHPHGDSSIYEALVRMSQDWKLRAPLIEMHGNNGSMDGDPPAAMRYTEARLAQISGLMLADIDKETVEMTWNFDDTEKEPTVLPARIPNLFVNGATGISAGYATEIPTHNLGEMIDASIYLIDHPNASLDKLMEFVPGPDFPTGGIIQGVKEIKKAYETGRGRVVVRAKTAIEDLRGGRQQITVTEIPYEVNKAQLVKRINDLRINKKVEGIADARDETDRNGLRIAIELKRGANATGVLNYLLKNTDLQINYNFNMVAIDDQRPMRVGLKHYLASYVDFQKEIVTKRTRFDLNKAQARLHIVEGLIKALSILDQVIKTIRASKNRRDAQDNLVSAYQFTQRQAEAIVALQLYRLTNTDVTALENELANLNERIAKFKQILLDDHELALVIKQELRAIKKSFDSPRQTQIENEVQKLTVDTKITVANEEVMVLVSHAGYIKRSSMRSFNASDFSDNGLREDDYPLLVTKTSTLSHLFMFTNQGNVIYRPVHEVMDAKWKETGEHISQTIGLADDEEIITTLVVDQLEQAGSILMASSDGQVKQSAFADFKPGSRYKSRPTLAFKLKSSDARVVAVHYYDPLGPKQSLVAISKAGYGLRFAVDELPVTGLRTSGVRAINLKDDDQLVDLALVEESDHLALITQRGAFKEMLASDLELGARARRGNLVLHKLKKNPHEIADFIGHAADYQGALEIITSRPVFQDVLVADHHLGTTKSNGTFVIDTDTQGVPVLIRKKIIISQPNDDEHENDQSIEQLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI27</accession>
    <name>H4GI27_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Exonuclease SbcC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_6363</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000011">
        <property type="protein sequence ID" value="EHS87437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121711.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000011.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6363"/>
    <dbReference type="EnsemblBacteria" id="EHS87437">
        <property type="protein sequence ID" value="EHS87437"/>
        <property type="gene ID" value="PS3_6363"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.166"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_23" evidence="2">
        <location>
            <begin position="5"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="187"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="458"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="546"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="650"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="881" mass="99763" checksum="318AC220F1A5816A" modified="2012-04-18" version="1">MRPLKLELNYFGPYGHATIDFSQFSEVPVFLISGKTGSGKTTLFDAMSFALFGKTSNDDREAKLLRSDFAPDDQESSVKFWFEHQGVQYQLERRPKQRIRRSSGKWVELGGKVSLIYPLDAAEPIEKTKDGDVKAVIVALLNLNADQFRQIVLLPQGKFRQFLESSSKDKEAILRSLFGTQLYERWVDDLQNQYKQLKAASDQQTTQLTTLMQQIEEIEAEQPQEWLSDAQELFQKDQSALAKTQEQLATLQAQVQTLTAADRQWQSWQQAQTELEQTQVKQQALIEEQPAMDQVAKQIQLDEWVQGQVPKLNQYHRQHQLIDELSQKNQLAQQEWDHNQQVVTETQAQLKQLGNLDQAQVHLMQEIGQWAQLANDFQRLQTALKHYQDTKSNQASLESQLQVASQSVQEASERYTSINDQYARSMISRLAQRLEPGSPCPLCGSREHPHPVVVDELIVVSEDEVKQAQSHLDKVRAQNSQLQAQFEQTSQQLQEEQAQIDQQVAQLNQAMDVTDQDPDSLGKIIQDQQAAQESAQKHLTSQLKERDQWQAALTEAQGKLDQLKGQQVTLAEQLTSTEQEQGALKAQLMTALGQAPKLLSWEQMEEIADQLDRLPQLRQQLSDYQGAVKVVESRLAELKPQVAKQPAGEADQVRAELKDQQAQVEETAQAVGRLEARLHQHQQTISQVDQIMAQQTTMMTKLRSLNQLVQTLRGMGDKKLGLERFVLQQYFNEVLTVANQRFSQLTNDRYKFVLDQELTARATQNGLEVDVYDDYAGKARSVHTLSGGESFMASLALALALGEVVQQRQGGVQIDALFVDEGFGSLDQEALDEALTALQSIEGQRMVGIISHVTELEERIPDQLKVTATDGRSQVGYQHEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI33</accession>
    <name>H4GI33_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Arginyl-tRNA synthetase</fullName>
            <shortName evidence="8">ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">argS</name>
        <name evidence="12" type="ORF">PS3_6171</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000011">
        <property type="protein sequence ID" value="EHS87443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121717.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000011.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6171"/>
    <dbReference type="EnsemblBacteria" id="EHS87443">
        <property type="protein sequence ID" value="EHS87443"/>
        <property type="gene ID" value="PS3_6171"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.173"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="PTHR11956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="SSF55190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arg_tRNA_synt_N" evidence="11">
        <location>
            <begin position="5"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="DALR_1" evidence="10">
        <location>
            <begin position="449"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="562" mass="63909" checksum="3309493310FD28C0" modified="2012-04-18" version="1">MNEQAQIAMTLQTVLPELSVAEIESKIERPKDSNNGDFAFPTFFLAKQLHQAPQAIAADLIAKLDTSKYEKVVQAGPYINFYMNQGQVGADILKAILADPANYGRRDLGHNAPASVEFSSPNIAKPMGMGHLRSTMIGESIARILEKEHYKPIRIDYLGDWGTQFGKMMAAYKMWGSEEEVAKDPINVLLSYYVRISREADEHPEYDDMGREWFAKLEHGDEEAWQLWRWFRSISLERFQRVYKMLDVNFDSFNGEAFSAQKMGESIQLLRDKKLLKKSQGAEIVDLEKYDLPPLLIIKSNGTSTYITRDIATALYRKRMFGHAKSLYVVGAEQEAYFNQLKAGLKEMGFNWYDQIEHISFGLMSINGQKMSTRKGNTVSLEDVLNDSIDLARQQIAEKNPDLANADEVAKQVGVGAVIFHDLKNNRRNAVDFNLDEVVKFEGETGPYVQYSRARAESILRKGGLRDFSQVDLTKIGAEEWELVSFMGRFADVIQRAADNYDPSVIAKFALELAKRFNRYYAHTRILVDDEQRDARLAVVQAISHVLKYALLMLDIKAPDEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI47</accession>
    <name>H4GI47_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Gluconate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_6152</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGGY kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121731.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6152"/>
    <dbReference type="EnsemblBacteria" id="EHS87386">
        <property type="protein sequence ID" value="EHS87386"/>
        <property type="gene ID" value="PS3_6152"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.192"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="Carb_kinase_FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="Carb_kinase_FGGY_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FGGY_N" evidence="2">
        <location>
            <begin position="3"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="FGGY_C" evidence="3">
        <location>
            <begin position="257"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="503" mass="55569" checksum="009971576B4A6008" modified="2012-04-18" version="1">MKYYIGVDIGTTSTKAVLYDQDANVINKFQQGYRLYRDQDGMAEQDPEEIVQAVLQMIHDAAGLTNEEDNQLTSVSFSCANQSLILLDQDHHPISRVMTWADTRARRQAEALKQSSKAKTIYEKTGTPIHPMSPLIKLMWLKEIKPDLIKQASFYADIKSYLFWRLFGQFRIDVSIASCTGLMNLETQNWDPDILAMLHVSVDQLPEIVDSIDQAVGLSPVMAGLMGVDAEVPFVYGAFDGALSNLGVGAIQQDTVAITIGTSAAVRVICDHPVIDPHQRLFCYSVNRHLWVIGGPINNGGDVFQWAVEELIEDQIQAKYDVANRLIDQVPAGAHGLLFHPFLGGERAPIWEANARGSFVGLNPLHTRADMLRAVMEGICFNIATVFDAVQTMVGKPKRITATGGFANSKVWRQMLADVLDCPVNVPKSFESGCLGAVVMAMQSLGELSNLADVTSMVGQVEAYLPDEAVAALYERYLLVFKQVEEQLLPTYAALVELQEDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI69</accession>
    <name>H4GI69_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 6">50S ribosomal protein L20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rplT</name>
        <name evidence="7" type="ORF">PS3_13777</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the bacterial ribosomal protein bL20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121753.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13777"/>
    <dbReference type="EnsemblBacteria" id="EHS87408">
        <property type="protein sequence ID" value="EHS87408"/>
        <property type="gene ID" value="PS3_13777"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.222"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07026">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00382">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005813">
        <property type="entry name" value="Ribosomal_L20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035566">
        <property type="entry name" value="Ribosomal_protein_L20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10986">
        <property type="entry name" value="PTHR10986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00453">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00062">
        <property type="entry name" value="RIBOSOMALL20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74731">
        <property type="entry name" value="SSF74731"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01032">
        <property type="entry name" value="rplT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00937">
        <property type="entry name" value="RIBOSOMAL_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="117" mass="13399" checksum="C187E8D4FFB17386" modified="2012-04-18" version="1">MRVKGGTVTRARRKRIMKLAKGYTGSKHRLFKTAKDQVMKSGTYAFRDRKVNKRNFRQLWITRINAGARMNDISYSKLMHGLKLANIDLNRKMLADLAVNDADAFKALVDEAKKALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI80</accession>
    <name>H4GI80_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine-N(7)-)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA(m7G46)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trmB</name>
        <name evidence="2" type="ORF">PS3_3027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="1"/>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121764.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3027"/>
    <dbReference type="EnsemblBacteria" id="EHS87419">
        <property type="protein sequence ID" value="EHS87419"/>
        <property type="gene ID" value="PS3_3027"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.236"/>
    <dbReference type="UniPathway" id="UPA00989"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01057">
        <property type="entry name" value="tRNA_methyltr_TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="PTHR23417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00091">
        <property type="entry name" value="TIGR00091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Interaction with RNA" evidence="1">
        <location>
            <begin position="124"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="192"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="213" mass="24857" checksum="097E95B194A5BD3C" modified="2012-04-18" version="1">MRVKHKKWADPYLESHPETVSINPTEYKGKWQNRFEKDQPLHLEVGMGKGQFIIGMAQKYHQFNFIGLEIESTVAAIAAKKAEPLALPNLTFVRADGAGLDTYFAEDEIQQLYLNFSDPWPKTRHEKRRLTYKSFLTNYQRVLVDQGCIEMKTDNMGLFEYSLQSLNNYGMVFDGVWLDLHHSDEQENNVETEYEQKFAAKGQPIYKLRAHFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIE8</accession>
    <name>H4GIE8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">L-2-hydroxyisocaproate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_5396</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. LDH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121832.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5396"/>
    <dbReference type="EnsemblBacteria" id="EHS87369">
        <property type="protein sequence ID" value="EHS87369"/>
        <property type="gene ID" value="PS3_5396"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.344"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018177">
        <property type="entry name" value="L-lactate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="SSF56327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00064">
        <property type="entry name" value="L_LDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ldh_1_N" evidence="5">
        <location>
            <begin position="4"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Ldh_1_C" evidence="6">
        <location>
            <begin position="149"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="314" mass="33883" checksum="D335F195F356B5B5" modified="2012-04-18" version="1">MTRKVGVIGLGHVGTAIANYLLVTGAVDELVLVDTNELKVNAEATDFEDAAANLNWHSKVVVNDFAALADADVVVSSVGAISLQNDDAKHDRFVELKFTSQAAKEVGTHLKEVGFNGVLVSITNPCDVITALYQRFTGLPTDQVIGTGTLLDSARMKKAVGQALDIDPRSVTGYNLGEHGNSQFTAWSQVRALGHSLPELAETEGLDLDELAEVSRAGGYTVFHGKHYTNYGVASAAVRLINVILNDANAELPVSNWNEKYKTYLSYPAVVGNNGVVQQLKLNLTDEEEQQLAQSANYIKTRFDDVYAKLTAND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIG8</accession>
    <name>H4GIG8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9 10">Chromosomal replication initiator protein DnaA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dnaA</name>
        <name evidence="14" type="ORF">PS3_13412</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 11">Belongs to the DnaA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121852.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000017.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13412"/>
    <dbReference type="EnsemblBacteria" id="EHS87289">
        <property type="protein sequence ID" value="EHS87289"/>
        <property type="gene ID" value="PS3_13412"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.368"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06571">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1750.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00377">
        <property type="entry name" value="DnaA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001957">
        <property type="entry name" value="Chromosome_initiator_DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020591">
        <property type="entry name" value="Chromosome_initiator_DnaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018312">
        <property type="entry name" value="Chromosome_initiator_DnaA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013317">
        <property type="entry name" value="DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013159">
        <property type="entry name" value="DnaA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024633">
        <property type="entry name" value="DnaA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038454">
        <property type="entry name" value="DnaA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30050:SF2">
        <property type="entry name" value="PTHR30050:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00308">
        <property type="entry name" value="Bac_DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08299">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11638">
        <property type="entry name" value="DnaA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00051">
        <property type="entry name" value="DNAA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00760">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00362">
        <property type="entry name" value="DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01008">
        <property type="entry name" value="DNAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="12">
        <location>
            <begin position="135"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Bac_DnaA_C" evidence="13">
        <location>
            <begin position="346"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="143"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS87289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="438" mass="49190" checksum="814059F2352036CC" modified="2012-04-18" version="1">MTELDSLWEAIQNALRESTAEVTYTNLIAPAKAVSLVNNRLTIQLPTENHLDFWQDNLINKVRQLLYSQTGQPIEPIFILANGISQPVPTVAPTAQTSYETPLNPEYTFENFIEGRSNQMAYASAFAASEQPGGLYNPLLIYGGVGLGKTHLMQAIANNMLTHNPNARIKYVTSETFVNDYVNSIKNRSQEQFRQEYRNLDALLVDDVQFFASKGETQLEFFNTFNVLHDNNKQIVLTADQNPKQIPNLTERLVSRFVWGVPVEITSPELETRIAILQSKAEEEHIQAPNEVLNYIASKVNTNVRELEGALMRVRVFAELQKQPLTPQIAAEALQGLGFATETTLSIDLIQKKVAAYFNINQSDITGKKRSQNIVVPRQIAMYLSRELTDSSLPKIGKEFGGKDHTTVLHAIDKIEKNISSDSGLEKDLKNLRDELQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GII7</accession>
    <name>H4GII7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ErfK/YbiS/YcfS/YnhG family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_7162</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000018">
        <property type="protein sequence ID" value="EHS87282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7162"/>
    <dbReference type="MEROPS" id="C82.001"/>
    <dbReference type="EnsemblBacteria" id="EHS87282">
        <property type="protein sequence ID" value="EHS87282"/>
        <property type="gene ID" value="PS3_7162"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.389"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038054">
        <property type="entry name" value="LD_TPept-like_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022029">
        <property type="entry name" value="PG-bd_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12229">
        <property type="entry name" value="PG_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143985">
        <property type="entry name" value="SSF143985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PG_binding_4" evidence="4">
        <location>
            <begin position="187"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="YkuD" evidence="3">
        <location>
            <begin position="324"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="449" mass="48514" checksum="76F931FBA524B679" modified="2012-04-18" version="1">MVVAAVLAGINHHQNTYFNKNTSINGVNVGGLTSQQAYEKVKKTKLSNDVYFNGQKIYDGTETSSGITAKDKSKFTAALKKQKTFFPSSKTSNLTVKGSATDTSQQTAMKAAATTAINAYNADKTIAVDAQAVLKNGKISVTKAKSGNGLDVEKMLQEFNDQKYSKSVKLTKIVKTPVKADSQTVAKEKRKLEALQNKTVDFKVNNTTVKLSAKNLITSATYANGQYNIDTTAIKKKIAQINSKYATLGKSFKFKTHSGSTITTSNSGTYGWKIDPDAAASAIVSAFSSNKSMTLDAKDFIYGIGYNENGTGYGVTANDGIGDTYVEVSISAQHAWWYKDGKLVFDADVVTGTEDGSNATPKGVYYIMYKQQNTTLRGQNDNGSSYASPVSYWAPFTQSGCGFHDASWRTDWSSTAYLTGGSHGCVNMHTYDAGTAYADMEQNEPVVIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIJ9</accession>
    <name>H4GIJ9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycerol-3-phosphate dehydrogenase [NAD(P)+]</fullName>
            <ecNumber evidence="8">1.1.1.94</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">NAD(P)H-dependent glycerol-3-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gpsA</name>
        <name evidence="16" type="ORF">PS3_10192</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>NAD(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.94"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 13">
            <text>NADP(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:11096"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.94"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 12">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.94" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121883.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10192"/>
    <dbReference type="EnsemblBacteria" id="EHS87194">
        <property type="protein sequence ID" value="EHS87194"/>
        <property type="gene ID" value="PS3_10192"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.408"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009331">
        <property type="term" value="C:glycerol-3-phosphate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004367">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase [NAD+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00394">
        <property type="entry name" value="NAD_Glyc3P_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006109">
        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000114">
        <property type="entry name" value="Glycerol-3-P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 8 11" id="KW-0520">NAD</keyword>
    <keyword evidence="3 8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_Gly3P_dh_N" evidence="14">
        <location>
            <begin position="4"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="NAD_Gly3P_dh_C" evidence="15">
        <location>
            <begin position="185"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8 10">
        <location>
            <begin position="260"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8 9">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <begin position="9"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EHS87194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="338" mass="36303" checksum="E7E2002C1447DF82" modified="2012-04-18" version="1">MTEKVAVLGAGSWGSVLANVLVENGHQVKLWSRNADQVQEMNQDHTNHRYLPNLTYQVGLEATTDMTAAVKDASLVLIVIPTKGLREVTHHLAQVLSASGASPVIAHATKGLEQGSYKRPSEMIQEEIAATNRQGIVVLSGPSHAEDVSIHDMTAVTAASSDEQAAKFVQGLFMNHYFRVYTNDDVIGAEFGAALKNVIAIGSGALTGLGYHDNARAALITRGLAEIRRLGVSFGANPMTFIGLSGVGDLVVTATSKNSRNWRAGYQLGQGKPLDRVVAEMGMVIEGLSTVKVAHELAQKRQVKMPITEAIYEVVYNGQDIQKVLADLMTREGTSEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIK3</accession>
    <name>H4GIK3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Protein translocase subunit SecA</fullName>
            <ecNumber evidence="11">7.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">secA</name>
        <name evidence="16" type="ORF">PS3_11353</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer and homodimer. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="11">Distribution is 50-50.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11 12">Belongs to the SecA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.4.2.8" evidence="11"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121887.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11353"/>
    <dbReference type="EnsemblBacteria" id="EHS87198">
        <property type="protein sequence ID" value="EHS87198"/>
        <property type="gene ID" value="PS3_11353"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.413"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017038">
        <property type="term" value="P:protein import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18803">
        <property type="entry name" value="SF2_C_secA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01382">
        <property type="entry name" value="SecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000185">
        <property type="entry name" value="SecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020937">
        <property type="entry name" value="SecA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011115">
        <property type="entry name" value="SecA_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014018">
        <property type="entry name" value="SecA_motor_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011130">
        <property type="entry name" value="SecA_preprotein_X-link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044722">
        <property type="entry name" value="SecA_SF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011116">
        <property type="entry name" value="SecA_Wing/Scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036266">
        <property type="entry name" value="SecA_Wing/Scaffold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036670">
        <property type="entry name" value="SecA_X-link_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612">
        <property type="entry name" value="PTHR30612"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07517">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01043">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07516">
        <property type="entry name" value="SecA_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00906">
        <property type="entry name" value="SECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00957">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00958">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81767">
        <property type="entry name" value="SSF81767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81886">
        <property type="entry name" value="SSF81886"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00963">
        <property type="entry name" value="secA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01312">
        <property type="entry name" value="SECA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51196">
        <property type="entry name" value="SECA_MOTOR_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-1278">Translocase</keyword>
    <keyword evidence="9 11 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 11 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SECA_MOTOR_DEAD" evidence="15">
        <location>
            <begin position="1"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="87"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="417"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="103"/>
            <end position="107"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="492"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EHS87198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="789" mass="89849" checksum="FF7EA4B433F034E3" modified="2012-04-18" version="1">MANILRKWVESDRRELRRIARQARKVEEYADQMASLKDEELQAKTPEFKERLANGETLDDILPEAFAVAREGAKRVLGLYPFHVQIMGGIVLHGGNIAEMKTGEGKTLTATMPVYLNALAGKGVHVITVNEYLSERDAEEMGQLYHWLGLSVGVNGAEGTADEKRAAYAADILYSTNSEIGFDYLRDNMAVYQEDQVQRGLNFAIVDEVDSILIDEARTPLIISGPGTGTSKLYQQADRFVKKLTEEDDYKIDLESKTVSLTDDGIKKAEQYFNLDNLYDTDNTALTHHLDQALRANYIMMLDKDYVVRDGEVLIVDSFTGRVMEGRRFSDGLHQAIEAKEHVEIQEENKTMANITYQNLFRMYSKLSGMTGTARTEMEEFREIYNMDTISIPTNMPVARVDEPDLLYPTLTSKFHAVVRKIDELHTKGQPILVGTVAVETSEYLSHLLDEKKIPHVVLNAKNHAKEAEIVKNAGQVGAVTIATNMAGRGTDIKLGPGVRELGGLAVIGTERHESRRIDNQLRGRSGRQGDPGLSQFYLSLEDDLMKRFGGDRIKAFLQRLQVDDDDEDVVIKSRFLTRQVESAQKRVEGNNYDSRKNVLQYDDVMREQREIIYKERQQVITESESLDNVLMPMVERTVQRAVDQHTLGDQKDWDLNRIVDFASEVITKPEKLQVADLEGLSTTEIVEKLMTLANEVYDEKKEQLYDAAQMLEFEKVVILRVVDSHWTDHIDMMDQFRQSVGLRGYGQRNPLVEYQMAGYQMFEQMITDIEYEATRLFMKSEIRQNVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIM5</accession>
    <name>H4GIM5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Initiation-control protein yabA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_11356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121909.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11356"/>
    <dbReference type="EnsemblBacteria" id="EHS87220">
        <property type="protein sequence ID" value="EHS87220"/>
        <property type="gene ID" value="PS3_11356"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.439"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010377">
        <property type="entry name" value="YabA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06156">
        <property type="entry name" value="YabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021439">
        <property type="entry name" value="DUF972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="113" mass="13278" checksum="28CA4259BA8D114D" modified="2012-04-18" version="1">MTERVDKGNLYDRLNMITQQTKALVENMEILQEQMNSVLKENAELTIENNHLREMLAQQNVKADEGLSDSRKNLQKLYQEGFHVCNEYYGKRLEDDESCTFCLDVIFNEQRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIN2</accession>
    <name>H4GIN2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-specific adenosine deaminase</fullName>
            <ecNumber evidence="3">3.5.4.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tadA</name>
        <name evidence="5" type="ORF">PS3_14267</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>adenosine(34) in tRNA + H(+) + H2O = inosine(34) in tRNA + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:43168"/>
            <dbReference type="Rhea" id="RHEA-COMP:10373"/>
            <dbReference type="Rhea" id="RHEA-COMP:10374"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:82852"/>
            <dbReference type="EC" id="3.5.4.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.33" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14267"/>
    <dbReference type="EnsemblBacteria" id="EHS87227">
        <property type="protein sequence ID" value="EHS87227"/>
        <property type="gene ID" value="PS3_14267"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.446"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00972">
        <property type="entry name" value="tRNA_aden_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028883">
        <property type="entry name" value="tRNA_aden_deaminase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14437">
        <property type="entry name" value="MafB19-deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="4">
        <location>
            <begin position="5"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="164" mass="18862" checksum="0CEA4C0FF0A83F5E" modified="2012-04-18" version="1">MATEREITFYMKEALKEARDAALMNEVPIGAVIVHDGQIIGRGHNMRERYQDVTYHAEILAINEANQALRSWRLENCELYVTLEPCIMCSGAIINARIPRVYYGAADPKAGAVHSLYQLMNDQRLNHQVEVHAGIMEAPCQQLLKDFFRAIRRRKKILKQKPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIP3</accession>
    <name>H4GIP3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_10193</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121927.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10193"/>
    <dbReference type="EnsemblBacteria" id="EHS87238">
        <property type="protein sequence ID" value="EHS87238"/>
        <property type="gene ID" value="PS3_10193"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.462"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="331"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="354"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="416"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="440" mass="47969" checksum="6621950800D8F7B0" modified="2012-04-18" version="1">MQTSDQSTLKRSLGLTSALSIVVGTIIGSGIFFKQGSVLDSAGSSTMAIIAWIFGGIITLTAGLTIAEIGAQMPDTGGLYVYIENIYGRILGFLSGWMQIIVYGPAIIASVAGFMSILMVNFFGLSNFWRIPLAVITIVAIGLMNLLENKVGAAFSVITTIGKMLPIAVIIIVGLFWGNHNALGQTISGIHQSTGNFGVAVLATLFGYDGWILIASLGGEIKRPQKLLPLAIIWGIIIVLIIYTLITIAVFRFLPASQIHQLGENTVAYIANTVFGEIGGKLLSAGIIISMMGTINGKILTFPRIVYAMARRQDIPFSRPLSYLTPKGKSPVVATTFIIILATIMMIFFDPDHLSDLCVFTVYCFYTLAFFGIFILRRRNHFRPFSTPLYPWIPLISIFGGLFVIVSEILNDPQGVVLFLGIVIVGLPIFYLVKRYYRQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIP5</accession>
    <name>H4GIP5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L31 type B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpmE2</name>
        <name evidence="5" type="ORF">PS3_14639</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL31 family. Type B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121929.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14639"/>
    <dbReference type="EnsemblBacteria" id="EHS87240">
        <property type="protein sequence ID" value="EHS87240"/>
        <property type="gene ID" value="PS3_14639"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.464"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00502">
        <property type="entry name" value="Ribosomal_L31_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="L28p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002150">
        <property type="entry name" value="Ribosomal_L31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027493">
        <property type="entry name" value="Ribosomal_L31_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042105">
        <property type="entry name" value="Ribosomal_L31_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33280">
        <property type="entry name" value="PTHR33280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01197">
        <property type="entry name" value="Ribosomal_L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01249">
        <property type="entry name" value="RIBOSOMALL31"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="SSF143800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00105">
        <property type="entry name" value="L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="81" mass="9221" checksum="4DD86CEF149D4FFA" modified="2012-04-18" version="1">MKQGIHPDYHYVVFEDSATGKQFISGSTATSKETVKWEDGNEYPLIRVEITSDSHPFYTGKQKFTQVDGAVDKFNKKYGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIQ6</accession>
    <name>H4GIQ6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Zinc/iron ABC transporter ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_4603</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4603"/>
    <dbReference type="EnsemblBacteria" id="EHS87251">
        <property type="protein sequence ID" value="EHS87251"/>
        <property type="gene ID" value="PS3_4603"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.476"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="3"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="188" mass="20922" checksum="E6B49580ED62DB00" modified="2012-04-18" version="1">MVGENGVGKTTLVRALLKQLTPHAGQIARDPKVKIGYVPQFRNLDNEYPLSIRDFIALNLPMKMMPWLTAVERQQVDRIIKATNLSSIADQPLGLASGGEKQRTYLAQSLLSDPNLLILDESTASLDPDSKIELLSLVQKFQQSGLTVIFVTHDWELAQQFGTHFLHMEPGCYRNGTIDQLSQHLGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIR0</accession>
    <name>H4GIR0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribonuclease M5</fullName>
            <ecNumber evidence="9">3.1.26.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">RNase M5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ribosomal RNA terminal maturase M5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rnmV</name>
        <name evidence="11" type="ORF">PS3_12398</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for correct processing of both the 5' and 3' ends of 5S rRNA precursor. Cleaves both sides of a double-stranded region yielding mature 5S rRNA in one step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ribonuclease M5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.8" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121944.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12398"/>
    <dbReference type="EnsemblBacteria" id="EHS87255">
        <property type="protein sequence ID" value="EHS87255"/>
        <property type="gene ID" value="PS3_12398"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.480"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043822">
        <property type="term" value="F:ribonuclease M5 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01027">
        <property type="entry name" value="TOPRIM_RNase_M5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01469">
        <property type="entry name" value="RNase_M5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004466">
        <property type="entry name" value="RNase_M5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025156">
        <property type="entry name" value="RNase_M5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034141">
        <property type="entry name" value="TOPRIM_RNase_M5-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13331">
        <property type="entry name" value="DUF4093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00334">
        <property type="entry name" value="5S_RNA_mat_M5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 9" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Toprim" evidence="10">
        <location>
            <begin position="5"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="197" mass="21955" checksum="57A63AD5D5EC7F30" modified="2012-04-18" version="1">MDKIKEIIVVEGRDDTKRVHEALNADTFETNGSALSLDDLKRLGVLQAKRGLIVLTDPDFNGERIRKMISQKIPEAKHAFISRQAGVPKNHSGSLGVEHASPQAIRQAIQAAHGSHGLDKLPPTINQTDLMQAHLIAHPQAKQRRLQLGEILGIGYVNGKQLLRRLQMFQITAEELRSAIQQIDQKGSSDEKNNRNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIR5</accession>
    <name>H4GIR5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_18944</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121949.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18944"/>
    <dbReference type="EnsemblBacteria" id="EHS87260">
        <property type="protein sequence ID" value="EHS87260"/>
        <property type="gene ID" value="PS3_18944"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.485"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="320" mass="36566" checksum="D633A7FCC6A15008" modified="2012-04-18" version="1">MAGKKVPLKAATNELLDQVNQLYPTGTVFLDFEGDQVGYVRHDQAQQKVLPGGLMITLTDLTEPDYTASHELLHLLMLLQGFPQIFFQLSLGEDELDEQMMIMATDLYNVAMHTVVVDEQRRHDLITPQIEAEYLAGIHATLSDEGDQVDNERTLRLLTLLDALVFYGDHLDQVQADLNQRYPLALKTAQALYAQAFDKAVDSPYAMRRVIVRLFKLFDEQMRAWQLPPLHNTEFTTVSPVLSERQLRLSVKQVFEIFHSDMQNRESGKRAYVGLRRNDRQNSFVIDAPQGDTAAEFVSLYAQPVQEFLDQHGIPYIVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIU4</accession>
    <name>H4GIU4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">50S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rplF</name>
        <name evidence="9" type="ORF">PS3_8080</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121977.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8080"/>
    <dbReference type="EnsemblBacteria" id="EHS87174">
        <property type="protein sequence ID" value="EHS87174"/>
        <property type="gene ID" value="PS3_8080"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.525"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01365_B">
        <property type="entry name" value="Ribosomal_L6_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_L6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_L6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_L6_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019906">
        <property type="entry name" value="Ribosomal_L6_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002358">
        <property type="entry name" value="Ribosomal_L6_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="PTHR11655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF14">
        <property type="entry name" value="PTHR11655:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00059">
        <property type="entry name" value="RIBOSOMALL6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="SSF56053"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03654">
        <property type="entry name" value="L6_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00525">
        <property type="entry name" value="RIBOSOMAL_L6_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 5 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L6" evidence="8">
        <location>
            <begin position="12"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L6" evidence="8">
        <location>
            <begin position="90"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="178" mass="19447" checksum="9B0BCB4C68E15623" modified="2012-04-18" version="1">MSRIGYKEIDLPEGVEISQDGNVVTVKGPKGTLTREISPLITMKIDGNVVTFTRDSDVNTVRALHGATRANVANMVEGVTKGFKKTMKLVGVGYRAQFKGTTLTLTVGYSNPVEMEKPEDLEVAVPDNTTIEISGINKEHVGDFAAEVRAVRSPEPYKGKGIRYENEHIIRKEGKTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIV7</accession>
    <name>H4GIV7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">50S ribosomal protein L4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rplD</name>
        <name evidence="8" type="ORF">PS3_7753</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Forms part of the polypeptide exit tunnel.</text>
    </comment>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 6">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121990.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7753"/>
    <dbReference type="EnsemblBacteria" id="EHS87187">
        <property type="protein sequence ID" value="EHS87187"/>
        <property type="gene ID" value="PS3_7753"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.539"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_B">
        <property type="entry name" value="Ribosomal_L4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_L4/L1e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_L4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013005">
        <property type="entry name" value="Ribosomal_uL4/L1e"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746">
        <property type="entry name" value="PTHR10746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="SSF52166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03953">
        <property type="entry name" value="rplD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="45"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="207" mass="22422" checksum="A195103DE0420241" modified="2012-04-18" version="1">MTSVALYNQDGSQNGTVELNDSVFAVEVNESAEFEAILRQRASMRQGTHAVKNRSAVRGGGKKPWRQKGTGRARQGSIRAPQFRGGGIVFGPTPRSYNYALPRKVRQLAIKSALTQKVNAENFVVVDSINFDAPKTKEFAGVMNNLKVTEKTLVVVTDDDKNAQLSGRNLPNLTVVTPAGVNILNVVDAQKIVVTKSALSQVEEVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIV8</accession>
    <name>H4GIV8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">50S ribosomal protein L3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rplC</name>
        <name evidence="7" type="ORF">PS3_12646</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L19.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121991.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12646"/>
    <dbReference type="EnsemblBacteria" id="EHS87188">
        <property type="protein sequence ID" value="EHS87188"/>
        <property type="gene ID" value="PS3_12646"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.540"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_B">
        <property type="entry name" value="Ribosomal_L3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_L3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019927">
        <property type="entry name" value="Ribosomal_L3_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229">
        <property type="entry name" value="PTHR11229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03625">
        <property type="entry name" value="L3_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="220" mass="24064" checksum="ED29200F1EAC9D1A" modified="2012-04-18" version="1">MTKGILGKKVGMTQVFTDNGELVPVTVVEVTPNVVMQIKTVENDGYEAVQLGFADKREVLSNKAEQGHATKANTTPKRFIAEIRDAELGDDIKVGDEIKADLFTEGETVDVTGITKGHGYQGNIHKDGQRRGPMAHGSRYHRRPGSLGAIINHVFKGKKLPGRMGNNTRTVQHLRIVRVDTENNVLLIKGNVPGANKSFVTIKNSVKANTKKSLSKQKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIW7</accession>
    <name>H4GIW7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid ABC transporter substrate binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_10572</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NlpA lipoprotein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122000.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10572"/>
    <dbReference type="EnsemblBacteria" id="EHS87139">
        <property type="protein sequence ID" value="EHS87139"/>
        <property type="gene ID" value="PS3_10572"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.556"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004872">
        <property type="entry name" value="Lipoprotein_NlpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30429">
        <property type="entry name" value="PTHR30429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03180">
        <property type="entry name" value="Lipoprotein_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="285" mass="31532" checksum="51684DF61C81ECD8" modified="2012-04-18" version="1">MTKKLRHRLYWGIGLLVIILAAVGSFYYQHQRNTNTITIGAIGPDVKVWEHIAASSQAKKAGLKIKVKSFTDGVSLNKATAQGQVDVNAFQSWSYLQDYNAKNKRAPLVALGTTYLEPMGLYSDRYQKVSQLPDGATIAIANNPANTARGLLLLEKAGLITLKKSFTSTSGTNAIASNPKHLQFKEIDDTTGPRVIKSVDAVLIGNTIALQGHLNVLKDSIYHEAVNQSTKNNINVLATAKKNAHRSSYLKLVKLYHNRQIQKWIQEKYDGTKVEVNKPISYLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIY5</accession>
    <name>H4GIY5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetylglutamate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_20619</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000025">
        <property type="protein sequence ID" value="EHS87128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122018.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000025.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20619"/>
    <dbReference type="EnsemblBacteria" id="EHS87128">
        <property type="protein sequence ID" value="EHS87128"/>
        <property type="gene ID" value="PS3_20619"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.586"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00196"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000728">
        <property type="entry name" value="NAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="243" mass="26097" checksum="650F0FDA58411148" modified="2012-04-18" version="1">MKSHPVIIKIGGNTIPNLDQEFYTFLHQLQMQNRSIIIIHGGGPMISSLSEQLHLPVVKKNGIRVTDATTLAIAKMVLLGQAQPLLAQQLSAHGFKVVCKNAAMNHLLTGQAIDQATYGSVGTITKVNQTALQTPASQITVLAPLALDDQENWLNVNADEAAATIATLIHAEKLYLMTDVAGVLYEGHLISQLNHHLADQLASSHVIRSGMVPKIKAAFKAKTAGVNQVFITNQLCHPGTAIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIY7</accession>
    <name>H4GIY7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-oxoacyl-[acyl-carrier protein] reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_20476</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000025">
        <property type="protein sequence ID" value="EHS87130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20476"/>
    <dbReference type="EnsemblBacteria" id="EHS87130">
        <property type="protein sequence ID" value="EHS87130"/>
        <property type="gene ID" value="PS3_20476"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.589"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="257" mass="27598" checksum="EFC8E10A1307533D" modified="2012-04-18" version="1">MTEYFDLKGQVALVTGCSAGLGVQMAKALASQGANIVALARRKERVEAVAQEIHDEFGVETLPVQCDITDTERVNASVDEVLDHFGRIDILVNNAGTGAVAPAEDITDDQFENEMNIDLLGTFRVARAVAKKAMIPAKYGRVINIASMYGLVGNKVAGSSPYHAAKGGVVNLTRALAAEWGKYKITVNAICPGYFYTDLTRETLDSDFFQANAKTMIPEERYGESGELDTAVIFLASPKSTYVTGVPLPVDGGYTCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ00</accession>
    <name>H4GJ00_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">FAD:protein FMN transferase</fullName>
            <ecNumber evidence="3 9">2.7.1.180</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_13752</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Flavin transferase that catalyzes the transfer of the FMN moiety of FAD and its covalent binding to the hydroxyl group of a threonine residue in a target flavoprotein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>FAD + L-threonyl-[protein] = AMP + FMN-L-threonyl-[protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36847"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11061"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:74257"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.1.180"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ApbE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.180" evidence="3 9"/>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122033.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13752"/>
    <dbReference type="EnsemblBacteria" id="EHS87112">
        <property type="protein sequence ID" value="EHS87112"/>
        <property type="gene ID" value="PS3_13752"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.607"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017013">
        <property type="term" value="P:protein flavinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.520.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024932">
        <property type="entry name" value="ApbE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003374">
        <property type="entry name" value="ApbE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30040">
        <property type="entry name" value="PTHR30040"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02424">
        <property type="entry name" value="ApbE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143631">
        <property type="entry name" value="SSF143631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="5 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="253" mass="28013" checksum="B2F8ACC1E5236E0F" modified="2012-04-18" version="1">MIKSQVIKMMTIPFTISIAASTDDVLNETFALIMKPIRQRLAQVEQNYSAFRADSLVTRFQNGEDQLLIENSEFQQIYAATKEAQLQTEGYFDPMFSGKFDPTGYVKGWAITRIFNESLRPLLADPRIDGVCLNGGGDLQVASRSDWDFTCHIGIENPQDLQKFIGAYAIANGAVASSGVSKRGHHIKVFGEADLLQVSIIDPDLVMADVWATAGIAAGWDRFKELIKDHRLSGMAVTKSKIIEFDRGVMTNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ35</accession>
    <name>H4GJ35_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">ATP synthase subunit a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP synthase F0 sector subunit a</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">F-ATPase subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">atpB</name>
        <name evidence="13" type="ORF">PS3_14627</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Key component of the proton channel; it plays a direct role in the translocation of protons across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11 12">Cell membrane</location>
            <topology evidence="11 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 12">Belongs to the ATPase A chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122068.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14627"/>
    <dbReference type="EnsemblBacteria" id="EHS87060">
        <property type="protein sequence ID" value="EHS87060"/>
        <property type="gene ID" value="PS3_14627"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.673"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01393">
        <property type="entry name" value="ATP_synth_a_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045082">
        <property type="entry name" value="ATP_syn_F0_a_bact/chloroplast"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000568">
        <property type="entry name" value="ATP_synth_F0_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035908">
        <property type="entry name" value="F0_ATP_A_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42823">
        <property type="entry name" value="PTHR42823"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00119">
        <property type="entry name" value="ATP-synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00123">
        <property type="entry name" value="ATPASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81336">
        <property type="entry name" value="SSF81336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01131">
        <property type="entry name" value="ATP_synt_6_or_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 11 12" id="KW-0138">CF(0)</keyword>
    <keyword evidence="6 11 12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 11 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS87060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="236" mass="26355" checksum="D07580BE3BFF7082" modified="2012-04-18" version="1">MGGDAFTFELFGLTFNWTNIISGTIICCIVFFVCFGLSRHLQMKPTKGQNMIEWVVDFTNGITRSQMNSTESAKYSYFIFILFVFLFVANQFGLIFQFGWNGHELLKSPTADPVITLTFSLLCVTLSHFAGIAQNGMKGYLKEFVSPFALFLPIKIVEEFSNFLTLGLRIFGNIYAGELLLGLIGQVAFSHGVWTMPIGLALEVVWQGFSVFIGSIQAYVFVTLTSVYISRKVSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ36</accession>
    <name>H4GJ36_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">YibE/F family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_6370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122069.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6370"/>
    <dbReference type="EnsemblBacteria" id="EHS87061">
        <property type="protein sequence ID" value="EHS87061"/>
        <property type="gene ID" value="PS3_6370"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.675"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012507">
        <property type="entry name" value="YibE_F"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41771">
        <property type="entry name" value="PTHR41771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07907">
        <property type="entry name" value="YibE_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="341"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="376" mass="41632" checksum="B7C02686C356C94A" modified="2012-04-18" version="1">MILSWIKAHRLMLIMMIVIGIGAMFFVTHNQTLYRTPIAKVIAVKNGHPQKQTDQFENVDSETNQTLTVRMLNGRYRGQTLTVKNQFSQSGAMDQRYRVGDQIFLTQISKQHGQLSAYIKGYKRDVVLVFLTWLVIALLLLMMTTSGFFALLSVALNVVLFIIAIKLNELTKGDNALLIFSVLAVLFCFLSLLLVLGPNKKMLATFFATVIATSVSMLISLLVMAMTHDRGIYFESMEYVTQVPRSIFIAETLLGSLGAVMDESSDIIATLFELKEIDPEVTAGQLFISGRNIGKTIMGPLINVLFLIFMADTFTSALLYIKNGNNWGYTFAMNMSLGTVQSLVSGIGIVLAIPLVSAFAALLLGKRGKKCQQLQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ42</accession>
    <name>H4GJ42_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Threonylcarbamoyl-AMP synthase</fullName>
            <shortName evidence="7">TC-AMP synthase</shortName>
            <ecNumber evidence="4 7">2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_9422</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + hydrogencarbonate + L-threonine = diphosphate + H2O + L-threonylcarbamoyladenylate</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the SUA5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="4 7"/>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122075.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9422"/>
    <dbReference type="EnsemblBacteria" id="EHS87067">
        <property type="protein sequence ID" value="EHS87067"/>
        <property type="gene ID" value="PS3_9422"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.681"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038385">
        <property type="entry name" value="Sua5/YwlC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005145">
        <property type="entry name" value="Sua5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010923">
        <property type="entry name" value="T(6)A37_SUA5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03481">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004930">
        <property type="entry name" value="Tln_factor_SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00057">
        <property type="entry name" value="TIGR00057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="YrdC-like" evidence="9">
        <location>
            <begin position="8"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR004930-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="338" mass="36530" checksum="F5866FDC0B2BE003" modified="2012-04-18" version="1">METKIFQPQQIAEAAHLIQQGQLVAFPTETVYGLGADATNPQAVRQVYQAKGRPSDNPLIVHVSTPAMVAQYAADIPVKAQKLMTHFWPGSLTIILPIKPDVLDPVVTGGLQTVAFRLPDNQVTLEIINQAGVPIVGPSANTSGKPSPTTAQHVYHDLKGKIAGIVDDGPTQVGLESTVIDLSTDEPAILRPGVVTEAEIEQVIGPLRVDHHRVKANETPKAPGMKYKHYAPNAQVLMVADDDWELVKDQLTDSSVGILATDDHLKQLALADQIPTYSLGQDVASAAQRLFAGLRWFDDQPTIKTIYAQTFANQGVGEAYMNRLNKSAGGQYFYGSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ48</accession>
    <name>H4GJ48_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cardiolipin synthase</fullName>
            <shortName evidence="12">CL synthase</shortName>
            <ecNumber evidence="12">2.7.8.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PS3_3357</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) = a cardiolipin + glycerol</text>
            <dbReference type="Rhea" id="RHEA:31451"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:62237"/>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the phospholipase D family. Cardiolipin synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.-" evidence="12"/>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3357"/>
    <dbReference type="EnsemblBacteria" id="EHS87073">
        <property type="protein sequence ID" value="EHS87073"/>
        <property type="gene ID" value="PS3_3357"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.688"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008808">
        <property type="term" value="F:cardiolipin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01916">
        <property type="entry name" value="Cardiolipin_synth_Cls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030874">
        <property type="entry name" value="Cardiolipin_synth_Firmi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022924">
        <property type="entry name" value="Cardiolipin_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027379">
        <property type="entry name" value="CLS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13396">
        <property type="entry name" value="PLDc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04265">
        <property type="entry name" value="bac_cardiolipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="13">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="13">
        <location>
            <begin position="406"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="411"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS87073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="493" mass="57174" checksum="D239B06A2A249556" modified="2012-04-18" version="1">MLLSHSLWIIIILNTILAIWTVFRDRRDIAVTWAWLMVLTFLPIIGFIFYLFVGRKLSKDQIFNIRHEQKAVMDDFKVQQQNLIRSRSLLTKRKNLERDLLLSNLLLETDEAILTQGNQVQLYTDGNEKFDQLIQDINQATTQVHIEYYTFSSDHLGRRVLHALEEAARRGVEVRVLYDLGGSRGTNYRFFQYLERLGGEAQAFISSSKALFSSPRLNYHLHRKLVIIDGNIGYIGGFNIGDQYVGEDPKFGYWRDTHLRVTGSATVMMQIRFMMDWNTTCRRTKKTRIDIDAEYMRTQFERLAEYESRSQGVPMQIVSSGPDNENYAIRRGYQGIISQARQYVYIQTPYLIPEDSILESLIVAAKEGVDVRIMIPCMPDHPFVYRATEYFAKYLTDNGVKVYKYNHGFLHAKTMVSGNNIASVGSANLDYRSAKLNFEVNAFTYDHQLAGHLKAIFEADLKQSTLLTTDYFANQSRWLKLKQYFCRLLAPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ58</accession>
    <name>H4GJ58_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Esterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_11644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122091.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11644"/>
    <dbReference type="EnsemblBacteria" id="EHS87083">
        <property type="protein sequence ID" value="EHS87083"/>
        <property type="gene ID" value="PS3_11644"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.705"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="262" mass="29425" checksum="8E0476B9C29A4DAF" modified="2012-04-18" version="1">MEIKDYPINEHAQLTAYLQSTYPDQKLPAILIVPGGSMTHIPTESSEKTALRFNALGFQSLVLRYTFLSEQQPLLPQPILDLAAAVIMVKMHREEWHLNDQLTLLGFSAGGLIVSLYNDYWDSTWLTNQLGTHSAILKPSNAILGYPVIDLDLGFPNDVDTLHQWTNDPEFYSASHHVTDANVPTFLWAIMDDPLITVQNTINYSLALHAHQIPHEVHLFQHGPHGMTIANPLAAKPNEPDHADPHVAHWIDLANEWLQSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ80</accession>
    <name>H4GJ80_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Benzyl alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_20415</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122113.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20415"/>
    <dbReference type="EnsemblBacteria" id="EHS87035">
        <property type="protein sequence ID" value="EHS87035"/>
        <property type="gene ID" value="PS3_20415"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.735"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PKS_ER" evidence="4">
        <location>
            <begin position="12"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="364" mass="38471" checksum="8C44796E3CF04F14" modified="2012-04-18" version="1">MEIKTAVVQRQGALLEWTQAQLDEPKINEVLVKVVASGICHTDAKGRDTGITPYPVALGHEGAGIAEKVGAAVTTVKPGDHVVLSYTVDGTCPQCRTAHPGSCESFNLYNFGGASKDGNHRIHDEAGHDISNFFGQSSLSTYAVVDESGVVKVDPEVDLRYLGPLGCGFLTGAGTMLNYIKPQFGDTVAIFGAGAVGIAAIMGAKIMGCRHIIAVNRKDYRLTIAKEMGATEVINNSKVDPVAAINEIVPGGVDYVIDTTGVGSVILDGLHALRPGGECVLVGIGGDLNLNVMNDLLATSKKLTGVVEGDAVPQTFIPKLVDYFKQGKFPLDKLSRFYQPKDINQAFADSASGEVIKPIIVFDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ82</accession>
    <name>H4GJ82_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Aldehyde Dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_6943</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122115.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6943"/>
    <dbReference type="EnsemblBacteria" id="EHS87037">
        <property type="protein sequence ID" value="EHS87037"/>
        <property type="gene ID" value="PS3_6943"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.739"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004030">
        <property type="term" value="F:aldehyde dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07100">
        <property type="entry name" value="ALDH_SSADH1_GabD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044148">
        <property type="entry name" value="ALDH_GabD1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="4">
        <location>
            <begin position="2"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="457" mass="49975" checksum="87ABF87D278ED6E1" modified="2012-04-18" version="1">MTYQTINPYSNEVEKTFENTSAAEVEQAIVSARQLYLKWRTGDDLAARKAVIVKLGQLLRERRTSLAELMTRDMGKLIGEAEGEVDLCASFCDYYVEHADEFLAPQPIYTNSGNAHILKQALGVLMAVEPWNFPFYQIARVFIPNFLVGNPMLLKDASNCPTSAQAFADLVLEAGTPKGALTNLFVDYDQVAAIIADSRIAGVCLTGSERGGRSVATEAAKSLKKSTLELGGNDAFIVLEDADWDQLAQIAPQARLYNAGQVCTSSKRFIVPEARVDEFVEILKAAFKQVKMGDPMDRSTTLAPMCSKKAKEKLTKQVQTAIDNGAKLEYGFEPVTELEGQFFMPTILTGITEDNPLFDQEMFGPVAVVYGYQTEADAIALANNSSYGLGNTVFGSDIAHAREVAAQVESGMSWLNAGWASLPEIPFGGVKNSGYGRELSEIGFTEFVNQHLVYEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ88</accession>
    <name>H4GJ88_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NEAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_9009</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
            <topology evidence="1">Peptidoglycan-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122121.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9009"/>
    <dbReference type="EnsemblBacteria" id="EHS87043">
        <property type="protein sequence ID" value="EHS87043"/>
        <property type="gene ID" value="PS3_9009"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.747"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06920">
        <property type="entry name" value="NEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006635">
        <property type="entry name" value="NEAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037250">
        <property type="entry name" value="NEAT_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05031">
        <property type="entry name" value="NEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00725">
        <property type="entry name" value="NEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158911">
        <property type="entry name" value="SSF158911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50978">
        <property type="entry name" value="NEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003592622" description="NEAT domain-containing protein" evidence="4">
        <location>
            <begin position="34"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="NEAT" evidence="5">
        <location>
            <begin position="29"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="250" mass="27427" checksum="583A3AB6D5739077" modified="2012-04-18" version="1">MRKNQGINYIWRWMLSGLMFCLSFIGLLSVAHADSINYQTFKYGTSTTSMADGYYVKPAQITVSGNKYVITMTIRTANDLTAWPVVVNSIDGQAPQNVSKTRSGSSYDYSYSFTESDLNKVISSNISINVPGVYTATHNISFKFDTSDLPSKSGGNSQTESTTAVGTNDPSLNTKLKKLTSQASSQSKAASKKESQLAVSISQQNLATAKQNRYNAALFYYVLVIGLVGLVILIGSVGYFIWRGKYHPRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ89</accession>
    <name>H4GJ89_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_20429</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122122.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20429"/>
    <dbReference type="EnsemblBacteria" id="EHS87044">
        <property type="protein sequence ID" value="EHS87044"/>
        <property type="gene ID" value="PS3_20429"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.752"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="139" mass="15649" checksum="1400BEC9B747871A" modified="2012-04-18" version="1">MKQINVVGAVIIDDAGHVLISKRKAERVLGSLWEFPGGKIETGETPQAALKREMQEEFQDQIVVGPQVGTTAVHQYDFGEIHLSVYFAKLLTHHFNLLEHSKVEWVEQNELLKRDWPAADGEIVKELAQIDLSEVSFDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJB0</accession>
    <name>H4GJB0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sugar transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_19765</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000032">
        <property type="protein sequence ID" value="EHS87000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122143.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000032.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87000">
        <property type="protein sequence ID" value="EHS87000"/>
        <property type="gene ID" value="PS3_19765"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.803"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="24"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="308"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="396"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="457" mass="50276" checksum="6F071A0911741978" modified="2012-04-18" version="1">MDTKKTTSPRQALDTGLPDLPQRTLYAITFAFLGINMAFSLQSSQMSRICQTIGTDPNNLGFFFIFPPLMGMIVQPILGKMSDHTWHHRFGRRMIYLLFGTPIAALVLLMLPFSGSFGFGYGSLTAMIYACVAVCFMDLFSNICMQPSRMIVGDMVNNKQKNFAWSWQQIFSNTGAILATLLPFIFTAFGMSNTAKKGVVPNTVIWAYICAAVVLLVTGLWTVFNVKEYDPATYAKYHQIDPQIVQQKVSLWKLIRQAPRSFWEINLVQLFSWFAIMYGWTYTTGTLAKNVWHTTNVTSAGYQAAGNWYGVLTAISSIAAIIWGLFIAKTKPGSRKGWYTFGLIIGGIGMAALAFVTTKTATIIAMIMFGIGNLSINTAPFTLLTSSISGENEGAYLGLFNVGICVPQILASLCSFFIFPLVGHQQPMMILIAGIALLIGACFVPMIHEGVVKKAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJD8</accession>
    <name>H4GJD8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alkaline shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_9742</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asp23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000035">
        <property type="protein sequence ID" value="EHS86887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122171.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000035.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9742"/>
    <dbReference type="EnsemblBacteria" id="EHS86887">
        <property type="protein sequence ID" value="EHS86887"/>
        <property type="gene ID" value="PS3_9742"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.847"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005531">
        <property type="entry name" value="Asp23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34297">
        <property type="entry name" value="PTHR34297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03780">
        <property type="entry name" value="Asp23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="143" mass="15344" checksum="1A5430E9C41BF00E" modified="2012-04-18" version="1">MPEESTIVLNRDEDGNIQISPSVLEIIAGIAANEIDGVSKMHGSIANNVGALIGLSGGGHRRGVKLAHDPENLVIDVDVYLNYGVSVPKVATEIQTKVSQQIELMTDLHVSQVNVHVQGIVMPKQEQQVDPNNIFANDEEEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJF0</accession>
    <name>H4GJF0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_2282</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000036">
        <property type="protein sequence ID" value="EHS86873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS86873">
        <property type="protein sequence ID" value="EHS86873"/>
        <property type="gene ID" value="PS3_2282"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.875"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006724">
        <property type="entry name" value="Phage_TTP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04630">
        <property type="entry name" value="Phage_TTP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="244" mass="24955" checksum="CB565746CB9B3F3F" modified="2012-04-18" version="1">MANIGLKRIYVWLVGSDNKVLTGTNGLSDDGVYVVDTDANKGNLGLKTANITGLSGTVTKIAGNNVVVAVSNPPSAPSVAIDANAINFTIKQRLLGRVETSVKGAYVDGETIPKAGLIIESSSPTTGKSVFYGFGIGQFTEASQNVQTNTDTAEQRDDDNLTFTALGCPHFPNESPVIFGNAAENGFTKNAFFDLVAPGQTLVSASQDKTPGNVSASASALSGATTTLAGGGTTHPDTGSGNTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJF5</accession>
    <name>H4GJF5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA replication protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_2255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000036">
        <property type="protein sequence ID" value="EHS86878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122188.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000036.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2255"/>
    <dbReference type="EnsemblBacteria" id="EHS86878">
        <property type="protein sequence ID" value="EHS86878"/>
        <property type="gene ID" value="PS3_2255"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.899"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="89"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="260" mass="29079" checksum="6F0CB09CC872B3A7" modified="2012-04-18" version="1">MDGIKQPPVEITPEKRAEIQARIDGIPKKWRAILADQKRKEIADNSLWSGNQPLAFSFADWQPAKQPDEGLGRKVGNQAFLLAKKMEATPVNVNLFGTRGSGKTSLALAIVDKVAKETGQSWLFVNTTELASLIGQQYEYPELKGKLDRLYKAITGIKSGDGSWYRKPVDILVLDDFGTEGGQKSDNLKVRRDMQEWLYRASNARIDIQNNRPTGSTIITTNNISSELLAMYDSKLISRIVTKQPENVINFTKLKDVRNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJG8</accession>
    <name>H4GJG8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Xanthine/uracil/vitamin C permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_8067</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000039">
        <property type="protein sequence ID" value="EHS86611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8067"/>
    <dbReference type="EnsemblBacteria" id="EHS86611">
        <property type="protein sequence ID" value="EHS86611"/>
        <property type="gene ID" value="PS3_8067"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.919"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904823">
        <property type="term" value="P:purine nucleobase transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045018">
        <property type="entry name" value="Azg-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026033">
        <property type="entry name" value="Azg-like_bact_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337">
        <property type="entry name" value="PTHR43337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005353">
        <property type="entry name" value="PbuG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="374"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="436" mass="46085" checksum="2C0735D176A164AA" modified="2012-04-18" version="1">MSAISKYFKFAELGTNYRRETLAGFTTFISMAYILFVNPSVLGAAKMNTGAVFTATALASAFGCLMMGILARYPIATAPALGINAFFAYSVCIGMGISWQTALAGVFVASIIFIIITIFKLREAIIDAIPSDLKYAISAGIGLFIAFIGLHQGGLVVADKSTVVTLGSFTTPTTWLTIIGLLVTAVLMVMNVPGSIFIGMVVTAAVGLCLGLIPMPTHLLSAAPSLKPTFGVAISHLGDINTVQLWVVVLTFLLVTFFDTAGTLVGLAQQAGFMKDNKMPRVGQALMSDSSAMFVGSILGTSPVGAYVESSAGIAVGGRSGFASVVTGLLFIVGMFFSPLLTVVTDQVTAPALIIVGVLMAQNLRQIAWDKLEIAIPAFLIVLGMPLTYSVSDGMALGLITYPITMIAAKRGKEVHPIMYGLFFIFIIFIWVLNYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJI1</accession>
    <name>H4GJI1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ferrous iron transport protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PS3_3782</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Probable transporter of a GTP-driven Fe(2+) uptake system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. FeoB GTPase (TC 9.A.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000041">
        <property type="protein sequence ID" value="EHS86571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122214.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000041.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3782"/>
    <dbReference type="EnsemblBacteria" id="EHS86571">
        <property type="protein sequence ID" value="EHS86571"/>
        <property type="gene ID" value="PS3_3782"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.939"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055072">
        <property type="term" value="P:iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01879">
        <property type="entry name" value="FeoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003373">
        <property type="entry name" value="Fe2_transport_prot-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011640">
        <property type="entry name" value="Fe2_transport_prot_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041069">
        <property type="entry name" value="FeoB_Cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030389">
        <property type="entry name" value="G_FEOB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07664">
        <property type="entry name" value="FeoB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17910">
        <property type="entry name" value="FeoB_Cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02421">
        <property type="entry name" value="FeoB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00437">
        <property type="entry name" value="feoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51711">
        <property type="entry name" value="G_FEOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0410">Iron transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="340"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="392"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="421"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="450"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="513"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="603"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="637"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="669"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="domain" description="FeoB-type G" evidence="11">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS86571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="704" mass="78228" checksum="49933B4D352F3CFC" modified="2012-04-18" version="1">MEVVALAGNPNSGKTTLFNALTGSNQYIGNWPGVTVERKQGQLKGNHEETIVDLPGIYSLSPYTTEEVVARDFLINQRPHQIIDIIDSTNIERNLYLTLQLVETGVPIILALNMSDLLAKQGRRINQDKLSYLLQVPVCSISALKRSGIDDLKKYLGSDKSKPYAYPKYDNRLESALSEIEDELSGLVPDNDLRWYAIKAFENDEKVVPTLQLDAVKQQDIQEIIAITEQLFNDEADSIIVNARYDLIAKLVDMCVVDEADFKMSISDRIDLVVTNRFLALPIFALVMWGVYYVSIQTIGTMETDWVNDVFFGEWVPNWATAVLNSWGVATWMKGLIVDGIIAGVGSVIGFLPQIIMLFLCLGILEDCGYMARIAFVMDRIFHRFNLSGKSFIPILISTGCGVPGIMSTRTIESEKDRKMTIMLATFMPCSAKLTVIALITGTFFPEQSWVAPSAYFIGILAVIGSGIFLKKTSAFAGPPAPFVMELPAYHLPVIGNICRYVYARSTAFVKKAGTIIFVSCVVIWFLSSFNWRFQSVDENHSLLRSLGEIMAPIFAPLGFGDWHATVAVIAGLIAKENCVDTLRITFGSGSNVWFRHELLQSYSAIVGYSFLVFNLLCAPCFASIGTMYREYGDVKWTLRAVSYQTILAYIVAMTIYNGYQCLIGQATIWNVGLTVVGLLIVIYGIIIKREKSVVNSQSTLEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJJ9</accession>
    <name>H4GJJ9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">6-phospho-beta-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_18963</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122232.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18963"/>
    <dbReference type="EnsemblBacteria" id="EHS86509">
        <property type="protein sequence ID" value="EHS86509"/>
        <property type="gene ID" value="PS3_18963"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.971"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="Glyco_hydro_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="PTHR10353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="490" mass="56137" checksum="9B7B88CF0B41606B" modified="2012-04-18" version="1">MANFRDDFLWGGAVAANQVEGGYDEGGKGLSVTDIQTAGSLETPRYLTYTLNGQRGKQVALPGQGLPKGAKGAVFEDEYYPNHVAIDFYHRYKEDIKLFAEMGFKVYRLSIAWTRIFPNGSEAEPNQAGLDFYRRVFEELRKYDIEPLVTISHYEDPLALSEKYNDWQDRGMIDAYVKYATTLFKEYKGLVKYWLTFNEINSALLGLSTFGGDGNDEDYQHNYQKLHYQFVASAKAVQVAHQIDPNNVVGNMICGIVYYPGSPDPKDIMLNRYNWEQNILYCSDAQCKGEYPSFAQRLWDEHHVELDITEEDRQDLQKGKVDMYTFSYYMSNMVTTHEVKDQVGGNFAAGAKNPYLQYSEWGWATDPTGLQYYLETMYDRYHLPMMVVENGLGAVDKFEDGTVHDDYRIDYLRQHIQAMDAAVGNGVDLRAYTTWGCIDCVSAGTGQMSKRYGFIYVDRDDEGQGTLDRYPKDSFYWYQKVIQSNGNDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJK8</accession>
    <name>H4GJK8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Maltose epimerase</fullName>
            <ecNumber evidence="5">5.1.3.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_21308</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the interconversion of alpha and beta anomers of maltose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose = beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:10264"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="5.1.3.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>alpha-maltose = beta-maltose</text>
            <dbReference type="Rhea" id="RHEA:21228"/>
            <dbReference type="ChEBI" id="CHEBI:18147"/>
            <dbReference type="ChEBI" id="CHEBI:18167"/>
            <dbReference type="EC" id="5.1.3.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the aldose epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.21" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122241.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21308"/>
    <dbReference type="EnsemblBacteria" id="EHS86518">
        <property type="protein sequence ID" value="EHS86518"/>
        <property type="gene ID" value="PS3_21308"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.984"/>
    <dbReference type="UniPathway" id="UPA00242"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004034">
        <property type="term" value="F:aldose 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050558">
        <property type="term" value="F:maltose epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019318">
        <property type="term" value="P:hexose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018052">
        <property type="entry name" value="Ald1_epimerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015443">
        <property type="entry name" value="Aldose_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005096">
        <property type="entry name" value="GALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00545">
        <property type="entry name" value="ALDOSE_1_EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="78"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="178"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005096-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS86518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="345" mass="38577" checksum="3828D58B6C903A72" modified="2012-04-18" version="1">MELTVTKFGEYQDQDVMKYTMTNDHDVRVSILNLAGIWQEYSIPTADGHQNLLLSSDSVEGFMGNGLCLNKLIGRVANRIDHATFTIDQQTYHVEQNEGENNIHGGNNGWKENFWEVETKESADKLQIVLSKQFTEAMDHFPGTETAKVTYTLANDNSLSIDFEGSSDQPTLFNPTNHTYWNISDHNDVTGLELQLNSTDHLAVNTTKIPTGERIANAGTPYDFQQFSSLGQALSAMQATSDEGGFDDYFEVTPTDASTNQPVAQLYDPETKRRMKMYSDRNSVVIYTANGLSGDLGLSKPAQSWLAIAMEGQTLTDAINHDDFGNIVLRPEAPMHNHLRYEIEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJL1</accession>
    <name>H4GJL1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Guanylate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_15821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122244.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15821"/>
    <dbReference type="EnsemblBacteria" id="EHS86521">
        <property type="protein sequence ID" value="EHS86521"/>
        <property type="gene ID" value="PS3_15821"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.989"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="1">
        <location>
            <begin position="2"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="185" mass="21031" checksum="6746B6E9E3D928DE" modified="2012-04-18" version="1">MKKVFVIVGAAGTGKTTVQAYLQAQYQIAKVITHTTRPARAGEVDGVDYYFESPDSFQHLELLEQVEYDHYQYGSSREALERAWQGHPAAVIVLDSQGALTYCQQLGAQVVVIFLTVADPDILVQRLKMRGDDSQAIRSRINSQEFKRDLTLPDQLTDRAYVINNEDWTITRQQLDRLVLRELVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJM8</accession>
    <name>H4GJM8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_4770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000044">
        <property type="protein sequence ID" value="EHS86251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122261.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000044.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4770"/>
    <dbReference type="EnsemblBacteria" id="EHS86251">
        <property type="protein sequence ID" value="EHS86251"/>
        <property type="gene ID" value="PS3_4770"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1009"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008407">
        <property type="entry name" value="Brnchd-chn_aa_trnsp_AzlD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05437">
        <property type="entry name" value="AzlD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="133" mass="15263" checksum="7C04FFC747919B39" modified="2012-04-18" version="1">MDFLSTSTVVAAANHDYKFHILVIIIAALAAFVPRYFPILFFTNRKLPEWFNEWMKYIPVSLFASLVAQRLFLDSYKVVTDKVTLFGHQLILYGHLDHIIAGLFVLLIAYFTRSMVLSVILGLILVFIFASFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJP1</accession>
    <name>H4GJP1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Nucleoside transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_15030</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000045">
        <property type="protein sequence ID" value="EHS86242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122274.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000045.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15030"/>
    <dbReference type="EnsemblBacteria" id="EHS86242">
        <property type="protein sequence ID" value="EHS86242"/>
        <property type="gene ID" value="PS3_15030"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1030"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005337">
        <property type="term" value="F:nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008276">
        <property type="entry name" value="C_nuclsd_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011657">
        <property type="entry name" value="CNT_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002668">
        <property type="entry name" value="CNT_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10590">
        <property type="entry name" value="PTHR10590"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07662">
        <property type="entry name" value="Nucleos_tra2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01773">
        <property type="entry name" value="Nucleos_tra2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleos_tra2_N" evidence="7">
        <location>
            <begin position="8"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleos_tra2_C" evidence="8">
        <location>
            <begin position="193"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS86242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="400" mass="43922" checksum="412C1644BFB87471" modified="2012-04-18" version="1">MYLAINVLGLIVFLAIGFIFSKDRKHINWKAIAIMVVLNLILAWFLTSSAAGRAGVKAAADGFTWIVDISAKGTVFALGDWYNTKNLNFICSSLMPILMIVPMFDILTYFGILPWIIKWIGRGLSFLTGQAKFESFFAVEMMFLGNTEVLAISGMQLRKMSKERNLSIAMMSMSCVTASILGSYIQMMPGQFILTAVPINIVNALICTSLLNPVHITPEEDTIEKVGNTDDGKKEPFFSYLGDSILGAGRLILIIIANVIAFVALAGLIDAILGLFWKQLSLESILGVVMFPFSWLMGLNPHDAWILSQNMGLKLVTNEFVVMGEVTKVIGQYPAHLKAVFTVFMTSFANFSTLGMVMGAFKGLVGQEDNDYIAKNFAYMLLSGVLVSLLSAAFVGLFVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJP6</accession>
    <name>H4GJP6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Multifunctional fusion protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="12">GTP cyclohydrolase-2</fullName>
                <ecNumber evidence="12">3.5.4.25</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="12">GTP cyclohydrolase II</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="13">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
                <shortName evidence="13">DHBP synthase</shortName>
                <ecNumber evidence="13">4.1.99.12</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="12" type="primary">ribA</name>
        <name evidence="13" type="synonym">ribB</name>
        <name evidence="15" type="ORF">PS3_6372</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>GTP + 4 H2O = 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + formate + 3 H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:23704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="13">Binds 2 divalent metal cations per subunit. Magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 13">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the DHBP synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the GTP cyclohydrolase II family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the DHBP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.25" evidence="12"/>
    <dbReference type="EC" id="4.1.99.12" evidence="13"/>
    <dbReference type="EMBL" id="AICN01000045">
        <property type="protein sequence ID" value="EHS86247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122279.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000045.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6372"/>
    <dbReference type="EnsemblBacteria" id="EHS86247">
        <property type="protein sequence ID" value="EHS86247"/>
        <property type="gene ID" value="PS3_6372"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1035"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003935">
        <property type="term" value="F:GTP cyclohydrolase II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00641">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00179">
        <property type="entry name" value="RibA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00180">
        <property type="entry name" value="RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032677">
        <property type="entry name" value="GTP_cyclohydro_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000926">
        <property type="entry name" value="RibA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036144">
        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00925">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142695">
        <property type="entry name" value="SSF142695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00505">
        <property type="entry name" value="ribA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="GTP_cyclohydro2" evidence="14">
        <location>
            <begin position="209"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="13">
        <location>
            <begin position="27"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="13">
        <location>
            <begin position="139"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="244"/>
            <end position="248"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="287"/>
            <end position="289"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="site" description="Essential for catalytic activity" evidence="13">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="site" description="Essential for catalytic activity" evidence="13">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EHS86247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="391" mass="43031" checksum="97DCFCFEB87B9751" modified="2012-04-18" version="1">MDVKKFQAALSYLKTGGLVIVTDDESREAEGDLVGLGAVVTPGKVNFMTKYARGLMCAPVGPEIAQRLALPQMTADNTDPFGTAFTLSVDHRTTTTGISAYDRAATIKALADPQSQPADFYRPGHIFPLTAQKDGVLDRDGHTEAAVDLARLVGLSPVAYICEILQEDGHMARRPRLVEMADEYGLPLLTVADIQTYRRLQVSDELTPVKLPTAYGDFKLVPFPDETLALTKGNWTVDEPVLVRLHSECLTGDVFGSKRCDCGPQLHAAMEKVAEVGQGAIIYLRQEGRGIGLLNKLKAYHLQEDGMDTVEANQALGFAEDERDYQEAAQILRHLGITKVRLMTNNPDKIEQLKAAGIEVVERVPLEIPPTAANRRYLKTKQEKLQHYLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJP7</accession>
    <name>H4GJP7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Riboflavin biosynthesis protein RibD</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="10">Diaminohydroxyphosphoribosylaminopyrimidine deaminase</fullName>
                <shortName evidence="10">DRAP deaminase</shortName>
                <ecNumber evidence="10">3.5.4.26</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">Riboflavin-specific deaminase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="10">5-amino-6-(5-phosphoribosylamino)uracil reductase</fullName>
                <ecNumber evidence="10">1.1.1.193</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">HTP reductase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PS3_16592</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)-pyrimidinedione 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H(+) + H2O = 5-amino-6-(5-phospho-D-ribosylamino)uracil + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21868"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58453"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-amino-6-(5-phospho-D-ribitylamino)uracil + NADP(+) = 5-amino-6-(5-phospho-D-ribosylamino)uracil + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17845"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58421"/>
            <dbReference type="ChEBI" id="CHEBI:58453"/>
            <dbReference type="EC" id="1.1.1.193"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10 13">Binds 1 zinc ion.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">In the C-terminal section; belongs to the HTP reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">In the N-terminal section; belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.26" evidence="10"/>
    <dbReference type="EC" id="1.1.1.193" evidence="10"/>
    <dbReference type="EMBL" id="AICN01000045">
        <property type="protein sequence ID" value="EHS86248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122280.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000045.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16592"/>
    <dbReference type="EnsemblBacteria" id="EHS86248">
        <property type="protein sequence ID" value="EHS86248"/>
        <property type="gene ID" value="PS3_16592"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1037"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00401"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008835">
        <property type="term" value="F:diaminohydroxyphosphoribosylaminopyrimidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004794">
        <property type="entry name" value="Eubact_RibD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006769">
        <property type="entry name" value="RibD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00326">
        <property type="entry name" value="eubact_ribD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10 12" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="8 10 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="14">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="287"/>
            <end position="293"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EHS86248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="354" mass="39464" checksum="16FD64C2B0590277" modified="2012-04-18" version="1">MNDQAWMERALTAAQRGGLATWQNPQVGAVLVKDDHLLAIGYHHRYGDYHAERDALSQVTPDQARGGTLYVTLEPCNHSGKQPPCSHLVVDYGIKRVVVGQLDPHQIVAGKGVQYLRDHGIQVTIGVLEQEARAINPHYNFFFTHQRPWIALKQAVTLDGKVALGNGKRTAITNQAVYDWVHQERAHYQAILAGSQTVLTDNPTLGTTAKLEHQPWRVILDRRGRLFEQAHLKIWQTTVYPTLVYTETPISLTLPGVEIKQLAEVTPKTVVDDLAQQGLQSLYVEGGPQIHNAFYQADLWDESITYLSPKLIGGRGVTAFSGPEPGRLMELVNVEVQKLGSDLRIRGERKCLPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJQ2</accession>
    <name>H4GJQ2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="6">4.2.1.136</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ADP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nnrD</name>
        <name evidence="7" type="ORF">PS3_19156</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(6S)-NADHX + ADP = AMP + H(+) + NADH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000046">
        <property type="protein sequence ID" value="EHS86234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122285.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000046.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19156"/>
    <dbReference type="EnsemblBacteria" id="EHS86234">
        <property type="protein sequence ID" value="EHS86234"/>
        <property type="gene ID" value="PS3_19156"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1044"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017953">
        <property type="entry name" value="Carbohydrate_kinase_pred_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01049">
        <property type="entry name" value="YJEF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01050">
        <property type="entry name" value="YJEF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6" id="KW-0520">NAD</keyword>
    <keyword evidence="3 6" id="KW-0521">NADP</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="186"/>
            <end position="190"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS86234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="284" mass="30649" checksum="194DBBC553E59716" modified="2012-04-18" version="1">MQALSADILKEVITPRPVQSYKGTFGRCVLIGGNNQFGGAIIMATMAAVYAGTGLVTAATDPINITALRTRVPEAMVIDYQDFNQLATLLSSANSIVVGPGLGETETSLKILKFVFNQIHAKQNLVIDGSAITLVANHHLTLPKAQIIFTPHEMEWQRLSRIAIVDQTEVNNRAAQAKLGAMVMVKKHHTEIYTDQNVYQLTIGTPAQATGGMGDTLAGMVGAFTAQFRTDYQKSALAAVYAHSAIAEELAANQYVVLPTQISAQLPQFMHQISQPDEQPIGFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJQ5</accession>
    <name>H4GJQ5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Deoxyguanosine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_11347</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DCK/DGK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000047">
        <property type="protein sequence ID" value="EHS86205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122288.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000047.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11347"/>
    <dbReference type="EnsemblBacteria" id="EHS86205">
        <property type="protein sequence ID" value="EHS86205"/>
        <property type="gene ID" value="PS3_11347"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1058"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019136">
        <property type="term" value="F:deoxynucleoside kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01673">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002624">
        <property type="entry name" value="DCK/DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031314">
        <property type="entry name" value="DNK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01712">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000705">
        <property type="entry name" value="DNK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="dNK" evidence="5">
        <location>
            <begin position="2"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="6"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="154"/>
            <end position="158"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000705-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000705-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000705-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS86205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="243" mass="28116" checksum="86AAB2334D7ADE0C" modified="2012-04-18" version="1">MITLSGIIGSGKTSLTTLLADRLGTKPFFEPVDDNPVLPLFYKGNEIAAEKRAAGDQNATNPYAYLLQTFFLNRRFAMIKQAMQNDNNILDRSIYEDEIFMRMNTDMGNATKVEYDIYKSLLNNMLEELPYAAHKKAPDLMIMIDVSYETMVKRIKKRGREYEQIENDASLVDYYQTLLSYYDRWKDEYDASPLLIIDGDQYDFMNNTADQQTVLQSIYDELFNLGSIQLSQYQQLSNQLKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJS0</accession>
    <name>H4GJS0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amidase family enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_6521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000047">
        <property type="protein sequence ID" value="EHS86220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122303.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000047.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6521"/>
    <dbReference type="EnsemblBacteria" id="EHS86220">
        <property type="protein sequence ID" value="EHS86220"/>
        <property type="gene ID" value="PS3_6521"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1078"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="27"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="491" mass="53509" checksum="7034DC13ACDA2A67" modified="2012-04-18" version="1">MSTQNLMRQPAYRLAQLVRDRQVSATDLVNTAYQQIDRLNPDLNAVITTRRQQALVEAAQLKDTGQPFLGVPILLKGLGQSLAGEPSTNANRLFQDNRATVTSNYTRRLQAAGFIIIGQTNFPEFGFKNVTDSQMYGDAKNPFNPTYSPGGSSGGAASALASGMVSIASASDGGGSIRIPASWSGLIGLKPTRGRMPMGPSDWRSWQGASINFALTRSVTDTALFLESMQALQAAAVFQAPLIKAGLVQRLNQPDRQLKIGYLTQSPVGTPVNPEAQQAVLAAVDFLRQAGWTVEEINWPTNGDALIRSYYAMNAGETAHMFQEIEAAIGRSVTRDDMELLTWALYQTGKQISAVDYIEALSTWDHAAAQWAQLHASYPLVLCPTTATAAISLNEQLVSDENLALMNQIDELTPQAQRELIYDQWLPSLTHSPFTQQANLTGEPAISLPTHLTKAGLPIGIQLTAAKGQEEWLLQIAKLFEDHHQFKMLVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJS2</accession>
    <name>H4GJS2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_20879</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000048">
        <property type="protein sequence ID" value="EHS86172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20879"/>
    <dbReference type="EnsemblBacteria" id="EHS86172">
        <property type="protein sequence ID" value="EHS86172"/>
        <property type="gene ID" value="PS3_20879"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1086"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010315">
        <property type="entry name" value="DUF915_hydro-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06028">
        <property type="entry name" value="DUF915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="203" mass="23032" checksum="5AF1FB3B1E752C85" modified="2012-04-18" version="1">MVKRILKGLILTLLIATMLFLTVQVFLIQGTPSENIKKTNSHVKYSSTPTLLIPGWGGSGWTYSKFIKLVQKENVAQNALVVRVSPDCRVSVTGSLKNKANPLIQVIYTWNYDTTFKPQVKELRAVLETLHDQYHVDRLNVIGHSYGGTEFIHVLFEDAKIRREIQFEKVILLGALLRRVLGPASIFIRTCLNNPKMIITRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJV2</accession>
    <name>H4GJV2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">Methionyl-tRNA formyltransferase</fullName>
            <ecNumber evidence="4 8">2.1.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fmt</name>
        <name evidence="11" type="ORF">PS3_8644</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 8">Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(6S)-10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N-formyl-L-methionyl-tRNA(fMet)</text>
            <dbReference type="Rhea" id="RHEA:24380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9952"/>
            <dbReference type="Rhea" id="RHEA-COMP:9953"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:78844"/>
            <dbReference type="EC" id="2.1.2.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the Fmt family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.9" evidence="4 8"/>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122335.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8644"/>
    <dbReference type="EnsemblBacteria" id="EHS86139">
        <property type="protein sequence ID" value="EHS86139"/>
        <property type="gene ID" value="PS3_8644"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1130"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08646">
        <property type="entry name" value="FMT_core_Met-tRNA-FMT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08704">
        <property type="entry name" value="Met_tRNA_FMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00182">
        <property type="entry name" value="Formyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005794">
        <property type="entry name" value="Fmt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037022">
        <property type="entry name" value="Formyl_trans_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001555">
        <property type="entry name" value="GART_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044135">
        <property type="entry name" value="Met-tRNA-FMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041711">
        <property type="entry name" value="Met-tRNA-FMT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="SSF50486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00460">
        <property type="entry name" value="fmt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00373">
        <property type="entry name" value="GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl_trans_N" evidence="9">
        <location>
            <begin position="4"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Formyl_trans_C" evidence="10">
        <location>
            <begin position="205"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Tetrahydrofolate (THF) binding" evidence="8">
        <location>
            <begin position="111"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS86139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="320" mass="34751" checksum="4A42D661F0AF5258" modified="2012-04-18" version="1">MTSIVFMGTPQFAVPILQALIDDPQYDVLAVVTQPDRRVGRKHVLTPTPVKKLAVTAGIKVFQPEKINHSPEMEAIIDLSPDLMITAAFGQFLPSKLLSAAKIAAINVHGSLLPKYRGGAPIQYAVMNGDQETGVTIMYMVKAMDAGDMLAQQAIPITKDDDAGTMFDKLSLIGRDLLMQTLPQLVAGTIQAKPQDESQVVFSPNISREQERIDYQMTAQQIDEHVRGLRPSPVGNMVIDGIVTKIYDVTPLEENTSLAAGKVVRLGKHELVLAAGQGTTYRINQLKPAGKPLMDITAYLNGHQQLEVGAQAVTDESESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJV4</accession>
    <name>H4GJV4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coenzyme A biosynthesis bifunctional protein CoaBC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA/pantothenate metabolism flavoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Phosphopantothenoylcysteine synthetase/decarboxylase</fullName>
            <shortName evidence="3">PPCS-PPCDC</shortName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenoylcysteine decarboxylase</fullName>
                <shortName evidence="3">PPC decarboxylase</shortName>
                <shortName evidence="3">PPC-DC</shortName>
                <ecNumber evidence="3">4.1.1.36</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaC</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenate--cysteine ligase</fullName>
                <ecNumber evidence="3">6.3.2.5</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaB</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="3">Phosphopantothenoylcysteine synthetase</fullName>
                <shortName evidence="3">PPC synthetase</shortName>
                <shortName evidence="3">PPC-S</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="3" type="primary">coaBC</name>
        <name evidence="7" type="ORF">PS3_4009</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>(R)-4'-phosphopantothenate + CTP + L-cysteine = CMP + diphosphate + H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine</text>
            <dbReference type="Rhea" id="RHEA:19397"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="6.3.2.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-phosphopantetheine + CO2</text>
            <dbReference type="Rhea" id="RHEA:16793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="4.1.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="3">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 2/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">In the C-terminal section; belongs to the PPC synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.36" evidence="3"/>
    <dbReference type="EC" id="6.3.2.5" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122337.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4009"/>
    <dbReference type="EnsemblBacteria" id="EHS86141">
        <property type="protein sequence ID" value="EHS86141"/>
        <property type="gene ID" value="PS3_4009"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1132"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00353"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015941">
        <property type="term" value="P:pantothenate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02225">
        <property type="entry name" value="CoaBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005252">
        <property type="entry name" value="CoaBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="SSF102645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00521">
        <property type="entry name" value="coaBC_dfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3 4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 4" id="KW-0288">FMN</keyword>
    <keyword evidence="3 4 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="5">
        <location>
            <begin position="3"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="DFP" evidence="6">
        <location>
            <begin position="180"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenoylcysteine decarboxylase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenate--cysteine ligase" evidence="3">
        <location>
            <begin position="185"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="303"/>
            <end position="306"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS86141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="396" mass="42572" checksum="736F81227AC8834B" modified="2012-04-18" version="1">MAKIMLYVSGSVAAYKSVSLVRSLQKQGHELRVVMTKSAQEFVGQATFASLLHQPVLTDMWDQTNQGHVPHIELADWTELAVVVPATANIIAKLANGIADDTATSTLLATKAPKLVVPAMNNHMWDNPATQRNLKLLQADGITILDPDEGLLAEGYAGKGRMPAVERIVSVIQGLLFAPLNGQRLVATAGGTREPIDPVRFIGNRSSGKMGIEIARAAAQAGYQVDLIVGQVEVDLPKSERIHITKVETTEEMFRVVDQAFVGADGLIMAAAVADYKVDHPADQKIKKTSATSTLSVDLTQGPDILATMGAKKQASQFVVGFAAETDHLDDHAQAKLSRKHADVIVGNNVTQAGSGFGTNTNQVTIYQPNQAPQQWKQMSKAAVAKNLIQFIQELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJV5</accession>
    <name>H4GJV5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">DNA-directed RNA polymerase subunit omega</fullName>
            <shortName evidence="12">RNAP omega subunit</shortName>
            <ecNumber evidence="2 12">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">RNA polymerase omega subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 12">Transcriptase subunit omega</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">rpoZ</name>
        <name evidence="13" type="ORF">PS3_13780</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 12">Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10 12">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the RNA polymerase subunit omega family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2 12"/>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122338.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13780"/>
    <dbReference type="EnsemblBacteria" id="EHS86142">
        <property type="protein sequence ID" value="EHS86142"/>
        <property type="gene ID" value="PS3_13780"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1133"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00366">
        <property type="entry name" value="RNApol_bact_RpoZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003716">
        <property type="entry name" value="DNA-dir_RNA_pol_omega"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34476">
        <property type="entry name" value="PTHR34476"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="SSF63562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00690">
        <property type="entry name" value="rpoZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS86142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="87" mass="10054" checksum="454BCAE1A37F02EB" modified="2012-04-18" version="1">MILFPSVDKLLERVDSRYSLIMLASKRAHEIDKYREDKFRADHSEKPVDMSEDKPLLLDHYESNKSVGMALEEIEAGLVTIDTETKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJW5</accession>
    <name>H4GJW5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">2,5-didehydrogluconate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_10375</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000050">
        <property type="protein sequence ID" value="EHS86039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10375"/>
    <dbReference type="EnsemblBacteria" id="EHS86039">
        <property type="protein sequence ID" value="EHS86039"/>
        <property type="gene ID" value="PS3_10375"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1150"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="PTHR43827"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="4">
        <location>
            <begin position="25"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="3">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="284" mass="31968" checksum="B108AC036FBFB1CF" modified="2012-04-18" version="1">MVKLNDGNLIPQFGLGVYMVPAGSETETAVYDALKMGIRHIDTAHAYRNERSVGQAINRAISEGIVSREDVWVTSKLWPSEYGEGKTAAAIDKMLDRLNVGYIDLLLLHQQFGDYKGAWQDMEKAVQAGKVRSIGISNFESNNLEDLMAAATIKPAVLQVECHPYWQQTELKNRIADYGTVIESWYPLGHGATDLLNEPIFTKLGQKYGKTNAQIVLRWHIQIGNIVFPKTTNPQHMKDNFDIFDFSLSDAEMAEIAKLDGKKRYYNVSLEEQIKTLGNWAPED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJX1</accession>
    <name>H4GJX1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_20629</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS85988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122354.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20629"/>
    <dbReference type="EnsemblBacteria" id="EHS85988">
        <property type="protein sequence ID" value="EHS85988"/>
        <property type="gene ID" value="PS3_20629"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1159"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="PTHR43827"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="4">
        <location>
            <begin position="17"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="3">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="273" mass="31398" checksum="FA9093AF9E8AE9CD" modified="2012-04-18" version="1">METVRLNNGVTIPLLGYGTYQLPQSTGPELLEEAIKLGYRHFDSAQEYQTEAAVGEAIRHSQLARDQFFMTTKSWMDGYQATLDGIDESLERFGFDYFDLILIHWPQSHPLESYLALEKIYQEGKARAIGVSNFNSRQLDKLLANSSVIPVINQIETHLLWPQTRMAKYLHEHQIAHESWSPLGASHLKEMIKIPLVQTIARQHHVKPTQVLLRYLTQQGIVTIPMTTNHQHLVDNLSSLDFDLSTAELASLRQFDRGLQSMHWPSWMREDAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK04</accession>
    <name>H4GK04_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tyrosine-protein phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_19798</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:10137"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:82620"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the metallo-dependent hydrolases superfamily. CpsB/CapC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000053">
        <property type="protein sequence ID" value="EHS85876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122387.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000053.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19798"/>
    <dbReference type="EnsemblBacteria" id="EHS85876">
        <property type="protein sequence ID" value="EHS85876"/>
        <property type="gene ID" value="PS3_19798"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1226"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016667">
        <property type="entry name" value="Caps_polysacc_synth_CpsB/CapC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39181">
        <property type="entry name" value="PTHR39181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19567">
        <property type="entry name" value="CpsB_CapC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016557">
        <property type="entry name" value="Caps_synth_CpsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR016557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="257" mass="28496" checksum="8B67EAEBC0251658" modified="2012-04-18" version="1">MGYVDLHCHLLPNLDDGSPSMAASLELARDAVADGVSHALVTPHHLNRRYVNHKDAVIQATSDFQASLNESGIELTVFPGQEVHLSNKILTALDQDDILFADEGGRYMLLELPSNEVPHYTKSMIYQLQSRGITPMIVHPERNNELVAHPERLADLLELGCLSQITASSYLGTFGKDVERAANLMVNAGQGTIMASDAHVMEKRDYELGAAMRRLAKDLGRKVADDYQQNAKDILNGDPVRMTWEPIKQKKDFGCFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK28</accession>
    <name>H4GK28_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="8">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purC</name>
        <name evidence="10" type="ORF">PS3_10207</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP + L-aspartate = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000055">
        <property type="protein sequence ID" value="EHS85844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122411.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000055.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10207"/>
    <dbReference type="EnsemblBacteria" id="EHS85844">
        <property type="protein sequence ID" value="EHS85844"/>
        <property type="gene ID" value="PS3_10207"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1260"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01415">
        <property type="entry name" value="SAICAR_synt_PurC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033934">
        <property type="entry name" value="SAICAR_synt_PurC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001636">
        <property type="entry name" value="SAICAR_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00081">
        <property type="entry name" value="purC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01058">
        <property type="entry name" value="SAICAR_SYNTHETASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR_synt" evidence="9">
        <location>
            <begin position="5"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="238" mass="26875" checksum="3E683BAB8FE65CC1" modified="2012-04-18" version="1">MSELLYAGKAKEMWSTDDPEVLRVVYLDQATAFNGKEKEHFAGKGHAAHAISNLVFHYLKDHGITTHLLETISPTEDLVKKCEMVPLEFVTRNVVAGHFASRYGLDEGADLNPAVEETFYKSDPLDDPFINASSANALGIATFAEQEQMWTMCREINELLKALFEKAGMKLVDFKLEFGKLPDGTIILADEFSPDNCRLWDLKTNAHMDKDVFRRQLADLTETYDQVLARIQTALEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK73</accession>
    <name>H4GK73_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-beta hydroxysteroid dehydrogenase/isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_7759</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000061">
        <property type="protein sequence ID" value="EHS85634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122456.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000061.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7759"/>
    <dbReference type="EnsemblBacteria" id="EHS85634">
        <property type="protein sequence ID" value="EHS85634"/>
        <property type="gene ID" value="PS3_7759"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1324"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008030">
        <property type="entry name" value="NmrA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05368">
        <property type="entry name" value="NmrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NmrA" evidence="1">
        <location>
            <begin position="4"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="281" mass="31289" checksum="EDA95BEECB22EB71" modified="2012-04-18" version="1">MNYLVTGASGHLGHQIVEHLIPKLQKDDHLFGLVHRPRPADNIEYRPADFMDLDSLKRAFQKVNVLVYIPSKTYVTADRLQELKNVITAARDQHVDAIVAMSFIADQANNPFVMAPFYHQMPDLLAQSGLNYVILKDALYADPLVPYLPELIDRQAIIYPVGDQKMAFIAIDDCAEAFATAATNPTIRSSRQTYTLSQETAYTMPELAAIMSKVTGQTIGYQPVTTQEFGQIYAEDGDGDELASMYAGGAMGLLSTVTNDFEFITGRKPIDMENFLKANYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK86</accession>
    <name>H4GK86_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative hydrolase/acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_10808</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10808"/>
    <dbReference type="EnsemblBacteria" id="EHS85538">
        <property type="protein sequence ID" value="EHS85538"/>
        <property type="gene ID" value="PS3_10808"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1345"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="22"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="262" mass="29656" checksum="FEBDDE6AC3609ABE" modified="2012-04-18" version="1">MTIKLRDGKQLNVRRSGNGIPLVMIHGFGGYQQIWTDQWPLIDRLSLMGISYDLRDHGASSRDPHLTKIRTLIDDLAELLQAYQLDRPILMGHSMGASIIYGLMQLYPEIDLRGVIAIDQSPKMISSPTWSYGYCGATRANYRLLLQTRGNISETYQGLDSKVLNQLAIAKDQFPFDRREHLSLLYDHARQDWRVGLVTTTTPTLLVTGQYSPYFNGDFAETLKTKNASLQHVIVRQSGHCPMAEQPAIFNEVISNFLQSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK93</accession>
    <name>H4GK93_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Anion transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_8079</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. DIT1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122476.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8079"/>
    <dbReference type="EnsemblBacteria" id="EHS85545">
        <property type="protein sequence ID" value="EHS85545"/>
        <property type="gene ID" value="PS3_8079"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1353"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030676">
        <property type="entry name" value="CitT-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42826">
        <property type="entry name" value="PTHR42826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002457">
        <property type="entry name" value="DASS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00785">
        <property type="entry name" value="dass"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="488" mass="52877" checksum="8B15A7C094A35AAF" modified="2012-04-18" version="1">MKTLEKVNYKGFIWPLVVGVIMWCMTPIRPDGLSAIAWQMFTVFVATIIGCITKPLPISGTTLLGLVVTVLLGLAPVESIKSASGVVTNSGILSAFSNSASWLIAMAFIMAAGITKTGLGNRIAYNMIKLFGKRSIGIAYAITGLELIMGGLIPSNSARSGGVVWPIVESISETYDSKPDEKLRKKIGAFLDFMAFHANIISTALFVTGAAPNIVAQAMAAKAHYQMTWIGWFTTAIVPVVICAIIIPWLIYKMFPPEIKETPNAKAWSEGKLAEMGKMSVPEKIMTAVFVMAILMWVLAGFFDIQQFEAAYVAFLAVVILLITGVLKVDDLLKEKGAWNILIWLSILVFMASKLTDYGFIGWFAKTVQSGLHGISWGVVLAVLVLVMFYSHYFFASGTAHVTALYLPFLTVSVAAGAPLALSAMMLALTTTIMASTTHYANGPASILASTGYVSQSEWWKYNFVLGLVYLVIFGVTVPLWGKVIGLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKC0</accession>
    <name>H4GKC0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-galactosidase</fullName>
            <ecNumber evidence="3">3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_8088</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122503.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8088"/>
    <dbReference type="EnsemblBacteria" id="EHS85572">
        <property type="protein sequence ID" value="EHS85572"/>
        <property type="gene ID" value="PS3_8088"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1391"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036156">
        <property type="entry name" value="Beta-gal/glucu_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006101">
        <property type="entry name" value="Glyco_hydro_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023232">
        <property type="entry name" value="Glyco_hydro_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006103">
        <property type="entry name" value="Glyco_hydro_2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023230">
        <property type="entry name" value="Glyco_hydro_2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006102">
        <property type="entry name" value="Glyco_hydro_2_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006104">
        <property type="entry name" value="Glyco_hydro_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00703">
        <property type="entry name" value="Glyco_hydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02836">
        <property type="entry name" value="Glyco_hydro_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02837">
        <property type="entry name" value="Glyco_hydro_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00132">
        <property type="entry name" value="GLHYDRLASE2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49303">
        <property type="entry name" value="SSF49303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00719">
        <property type="entry name" value="GLYCOSYL_HYDROL_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00608">
        <property type="entry name" value="GLYCOSYL_HYDROL_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_2_N" evidence="9">
        <location>
            <begin position="43"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_2" evidence="7">
        <location>
            <begin position="230"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_2_C" evidence="8">
        <location>
            <begin position="337"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="625" mass="71490" checksum="B362FC68A136E502" modified="2012-04-18" version="1">MEASLQWLDDPTVFRVNQLSAHSDHQFFKNQEELEAGQSSYVQSLNGTWDFAFAENPQVRPVNFWEVDADRSDFTTIEVPSEIELQNFAQINYINTLNPWSGKVYRRPAYSLGDDYEAAGSFSEGIDNTVGAYVRKFDLPIDWQGLDVHVMFEGVERAMYVWLNGHFIGYAEDSFTPSEFDLTPYLQNKNNVLAVEVFKHSTASWLEDQDMFRFSGIFRDVSLLALPASHVVDLKVDAPYDPANQQGQLGLALQLTNDADQVQVTLADPDGEPILETSLTANDQWQLPLHDLGTVQAWDNQQPVLYQLTLTVIKDGEVQEVIPQVVGFRQVMINEQAVLMLNGHRLVINGVNRHEWNCHRGRAIMYEDMLADLQTFHENNINAVRTCHYPDQLAWYDLCDRHGIYMMAENNLESHGTWQKMGAIEPSDNVPGSLPQWHDVVIDRARSNYELLKNHPAILFWSLGNESYAGDNIADMNAFYKEHDPSRLVHYEGVCHTPEYRDRISDFESWMYLPPTKVAEYLANNPDKPFIECEYMHSMGNSVGGLGSYNDLIDQYPQYCGGFIWDFIDQAIEVTDPVTGQKSMRYGGDFDDRHADYEFAGDGIMFANRQPKPAIQEVKYYYGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKE8</accession>
    <name>H4GKE8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Nicking enzyme TraA protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_4754</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MobA/MobL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000064">
        <property type="protein sequence ID" value="EHS85440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122530.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000064.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4754"/>
    <dbReference type="EnsemblBacteria" id="EHS85440">
        <property type="protein sequence ID" value="EHS85440"/>
        <property type="gene ID" value="PS3_4754"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1450"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005053">
        <property type="entry name" value="MobA_MobL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040834">
        <property type="entry name" value="NES_C_h"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03389">
        <property type="entry name" value="MobA_MobL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18208">
        <property type="entry name" value="NES_C_h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0184">Conjugation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MobA_MobL" evidence="4">
        <location>
            <begin position="17"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="NES_C_h" evidence="5">
        <location>
            <begin position="275"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="654"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS85440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="686" mass="80448" checksum="955FF037CD796D94" modified="2012-04-18" version="1">MAIFHMSFSNISAGKGRSAIASAAYRSGEKLFDDKEGRHYFYARAVMPESFILTPKNAPEWASNREQLWNEVEKKDRKSNSRYAKEFNVALPVELSESEQKELLTKYVQENFVDQGMVADVAIHRDHQDNPHAHVMLTNRPFNPDGSWGLKAKTQYIKDENGKQLLTKSGFPKQRKIWLVDWDKKEKINEWRKNWALSVNQFLAQKNIPDRISEKSFVEQGIADTPMQHEGINSKRHERKAFNQQVKTYRKSKAGYKNMQEKVINRGHLDSLSKHFSFNEKRLVNELSHELKTYISLESLDDKRRLLFNWKNSTLIKHAIGEDVTKQLLTINQQESSLKKADELSNKVVDRTTKKLYPELDFEQITAAERRELIKETDSEQTVFKGSELNERLMNIRDDLLTQQLLTFTKRPYVGWKLLMQQEKEVRIKLKYTLMIHDDSLESLEHVDQGLLEKYSPTEQQKITRAVKDLRTIMAVKQVIQTQYQEVLRRAFPNGDFNELPMIKQEQAYTAVMYYDPALKPCKIETIAQWRENPPRVFNTQEHQQGLAYLSGQLSLDQLDNHHLQRVLKHDGTKQLFLGECKADPMIKNSQIEKIQKQLKEQQAKDDQYRKVNMGHYQPLNYKPVSPSYYLKTAFSNAIMTALYARDEDYERQKQAHGLKETEWAMTKKQRQHQTRNRHEDGGMHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKF2</accession>
    <name>H4GKF2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">PTS system galactitol-specific IIC component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PS3_3334</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000064">
        <property type="protein sequence ID" value="EHS85444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122534.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000064.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3334"/>
    <dbReference type="EnsemblBacteria" id="EHS85444">
        <property type="protein sequence ID" value="EHS85444"/>
        <property type="gene ID" value="PS3_3334"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1465"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015577">
        <property type="term" value="F:galactitol transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013853">
        <property type="entry name" value="GatC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013014">
        <property type="entry name" value="PTS_EIIC_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004703">
        <property type="entry name" value="PTS_sugar-sp_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37324">
        <property type="entry name" value="PTHR37324"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03611">
        <property type="entry name" value="EIIC-GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006304">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51104">
        <property type="entry name" value="PTS_EIIC_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="350"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="403"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="427"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIC type-2" evidence="10">
        <location>
            <begin position="6"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS85444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="474" mass="52047" checksum="D34DE41F96F2DBC6" modified="2012-04-18" version="1">MFQDVNAFFTAFGASVVVPVMIFIIALFLKVQPKMAMMSAFYAGVGLTGFGWIISEFTPVVTKIIKQMVSTTGIKLPVVDIGWQAGSLASFGSTVGLAFFVFGLLFELLIFALGITKVFMASNLWNNFGFMIWGTMAYYVTHNFWLSMGLSFFMLLYSLVLAEVQADRWSDYYGVKNATVAASHNIEQTIPAIFLDPLWNLLGFNRVKVTPEYFKNKLGVFGEPTTLGALLGLIIGILGNLHSLGTIKAWGQILQFAVQLAAVMTIFPLVTGVFAKAFAPLAEEIDKNRKKEVAEQGKEIDAIHDKKRWFLGVDDGVGYGEPATIIAGVILIPIMVVIAFILPGNRVLPVVDLISLPFMVESIVAVTRGNILKVIANGIVWFSLGLYASSWLGTIYTGTVSHYGVAIPAGVVLITSFNLIARPVNALIFAAFISQNPVWVGLCIVIYLVALYGLRRYRPQIWSYLNRMAEKNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKG0</accession>
    <name>H4GKG0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein of hypothetical function DUF125 transmembrane</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_10415</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000066">
        <property type="protein sequence ID" value="EHS85395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122542.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000066.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10415"/>
    <dbReference type="EnsemblBacteria" id="EHS85395">
        <property type="protein sequence ID" value="EHS85395"/>
        <property type="gene ID" value="PS3_10415"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1479"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:cellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="PTHR31851"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS85395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="226" mass="24298" checksum="A73764F4F5D629B5" modified="2012-04-18" version="1">MKRKMSLAQKVNILRASVMGANDGIISVAGIVIGVAAATQNPFAILISGLAGSLAGTISMAAGEYISVSTQKDSQKMALAVEGQRLKNDYASEFSFVKQKYLDQSIDPELAQAATQQLMDKDPIGTVVQERYGFNPREYTSPYDAAIASFISFPIGSILPIVAVTISPVHLRIWMTMLAVTFMLIITGYTAAVLGGSDRWKSAFRNVIAGLITMLITFVIGQLFAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKL0</accession>
    <name>H4GKL0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FeS_assembly_P domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_20290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122592.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20290"/>
    <dbReference type="EnsemblBacteria" id="EHS85179">
        <property type="protein sequence ID" value="EHS85179"/>
        <property type="gene ID" value="PS3_20290"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1567"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeS_assembly_P" evidence="1">
        <location>
            <begin position="14"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="106" mass="11908" checksum="40B4565D7EF8D62C" modified="2012-04-18" version="1">MANPTSESIQDRMIKALQTVIDPELGIDLVNLGLIYNVELDDNGKATIQMTLTTMGCPISEVLQQMIKSALMKVEEVTEVEIQLVWYPVWSPDKMSRLAKITLGYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKM2</accession>
    <name>H4GKM2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_14430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122604.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14430"/>
    <dbReference type="EnsemblBacteria" id="EHS85191">
        <property type="protein sequence ID" value="EHS85191"/>
        <property type="gene ID" value="PS3_14430"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1579"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="SSF54518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS85191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="188" mass="21360" checksum="0FA81D077C497C99" modified="2012-04-18" version="1">MQKLYLPDHQANEAATSVILTDNGRQRYLLTGKNGLRSNHLEVQNIQGTVLAAAHQLGWGLRSKYQLINNQQPVGTITKPFGIMPEVRYVSGLNWVIIGNALNKHYRVFRTGRLVFMMQPMILSNASYYELAITNEADEPLAILIATLINRWQRKYRRLPSKLYKPSLNGPALGYSYQLKTGANRTRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKM5</accession>
    <name>H4GKM5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Chloride channel protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_6158</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122607.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6158"/>
    <dbReference type="EnsemblBacteria" id="EHS85194">
        <property type="protein sequence ID" value="EHS85194"/>
        <property type="gene ID" value="PS3_6158"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1583"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="Cl-channel_volt-gated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="SSF81340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="6">
        <location>
            <begin position="426"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="514" mass="57479" checksum="92DB6322042CA7CE" modified="2012-04-18" version="1">MKEQRLVTLKYNNFRQIGLAIVIGILAGLIVSCFRLLIQKALNLVLLLFEFFHHQPIWLIPWAVLSVLMALFLGWLSQNYPDIKGSGIPQVEGQLMGEYEEAWWPVLWRKFLGGVFGIGAGLFLGREGPSIQLGATVGQGIVERRTLTPVERRIGIASGAAAGLSAAFNAPMASAMFILEEVYHNFSPLVWMSVFISALCSNFVSMQFFGLRPDLDVAHEAVLPAGQYWQLLLLGIVLGVMGRLYQYVVVRLGKWSRLVKWFKPQWYPVVPFLLVIPIAWYLPITLGGGNELIVNLTKLPVSVWFFLGLFVLRFVFSMISYGAEIPGGIFLPILTLGAVLGALYGAFMIRFMGLPQNLLPNFIIYAMAGYFACISKAPFTAILLITEMVGSLAHLMPLAVVSMVAYLVVDLLNGQPVYNEMLQNFLQHKKIMFKGMTQMTTSIYEGSRLVDKMIKEVEWPAGCLILSIWRGEEQIIPRGDTKLKVGDTLIVELTDQDQQVHKETINQWAHEMGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKM6</accession>
    <name>H4GKM6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Amino acid permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_15056</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122608.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15056"/>
    <dbReference type="EnsemblBacteria" id="EHS85195">
        <property type="protein sequence ID" value="EHS85195"/>
        <property type="gene ID" value="PS3_15056"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1584"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amoino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="436"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="AA_permease" evidence="7">
        <location>
            <begin position="22"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="467" mass="50652" checksum="068EEF40DFDD4C86" modified="2012-04-18" version="1">MSNKSSGAPELSRSMTAGQMEMISLGGAIGVGLFMGSTSTIKWTGPSVILAYALVGIVLYVVMRALGEMIYINPGTGSFADYAAEYVHPIFGYLAKWANVFEYIVVGMSEVVAATEYLQYWWPHLPAWVSGIVIILFLALANLVSVKAYGTMEFYFAMIKVVTIILMILLGFMVIFFGFGNGGHPTGFSNLWSHGGFFTGGWTGFFFAMSIVVGSYEGIELLGISAGEVANPQEAIVKSVKSVLFRILIFYVGAIFVIVTIYPWNQLGSVGSPFVSTFAKVGITAAASIINFVVLTAALSGANSGIYSSSRMLFKLAHEGDAPKIFGRISKRIIPDAAILGISGGILIGFILDILATVFNKSTSNMFVVVFSSSVLPGMIPWFVILLAEMRFRRNNPEIMATHPFKLPLYPLSNYFAFVMLALIVIFMFVNPDTRISVIVGALVLIVATIVYLIRYGKDGHPDLDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKP3</accession>
    <name>H4GKP3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Shikimate dehydrogenase (NADP(+))</fullName>
            <shortName evidence="4">SDH</shortName>
            <ecNumber evidence="1 4">1.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">aroE</name>
        <name evidence="7" type="ORF">PS3_48</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.25" evidence="1 4"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122625.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_48"/>
    <dbReference type="EnsemblBacteria" id="EHS84963">
        <property type="protein sequence ID" value="EHS84963"/>
        <property type="gene ID" value="PS3_48"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1611"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00087"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019632">
        <property type="term" value="P:shikimate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00222">
        <property type="entry name" value="Shikimate_DH_AroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041121">
        <property type="entry name" value="SDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011342">
        <property type="entry name" value="Shikimate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="PTHR21089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18317">
        <property type="entry name" value="SDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00507">
        <property type="entry name" value="aroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 4" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shikimate_dh_N" evidence="5">
        <location>
            <begin position="14"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="SDH_C" evidence="6">
        <location>
            <begin position="258"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate binding" evidence="4">
        <location>
            <begin position="22"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="133"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS84963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="290" mass="32111" checksum="75F2966E77541D72" modified="2012-04-18" version="1">MLNELDGHTILIGLMAYPIRHSMSPTMHNHAFHKLGLNYAYLAFEIDNEKLPGAIDAFRTLDMRGSNISMPNKQKVIPYLDKLDPATEMIGAVNTVVNDNGVLTGYTTDGIGFMKSLDVDGINIRGQKMVLAGAGGAATAIAVQAALDGVKEMSIFNRDDEFWESAKHNVEIINEKTDCHATLHRLEDQEDFKSEIDSAFIYCDATSVGMNPLQDQTLVTDPSWFRKDLVVYDTVYAPRTTKLMEVAEEAGVEHVYNGLGMMIYQGAAAFKLWTGEDMPVDYISEVIFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKP9</accession>
    <name>H4GKP9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8 9">Translation initiation factor IF-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">infB</name>
        <name evidence="12" type="ORF">PS3_5336</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8 9">One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5336"/>
    <dbReference type="EnsemblBacteria" id="EHS84969">
        <property type="protein sequence ID" value="EHS84969"/>
        <property type="gene ID" value="PS3_5336"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1619"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03702">
        <property type="entry name" value="IF2_mtIF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_B">
        <property type="entry name" value="IF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044145">
        <property type="entry name" value="IF2_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006847">
        <property type="entry name" value="IF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000178">
        <property type="entry name" value="TF_IF2_bacterial-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="PTHR43381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04760">
        <property type="entry name" value="IF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="SSF52156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00487">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01176">
        <property type="entry name" value="IF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 8 9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="250"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="G-domain" evidence="8">
        <location>
            <begin position="253"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="81"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="259"/>
            <end position="266"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="305"/>
            <end position="309"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="359"/>
            <end position="362"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS84969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="749" mass="82598" checksum="C61BBB367E97B447" modified="2012-04-18" version="1">MDVKSHMSSVSTEEAQQLRQSFTKPATEKKSVPKTKPASQAKAKPAKKTNEQHKSTHRASADNKSDAQSSNNDHHQRRQKPNGQNNQNNQNKSNQGNQNNNNNNRNRNRNNNNNNSNNGGNGAHWFDNGNGKKNKKGKKGKKNRNQRMKNTAPKQPTQRKEWPLPEVLEYTEGMNTQDLAKILHRSSAEIIKKLFMLGVMVNQNQSLDADTIEILAADYGIEAKEKVQIDVADIDHFFDEKVDNQENQVPRPPVVTVMGHVDHGKTTLLDQIRHSHITAGEAGGITQAIGAYQVRYNDKMITFLDTPGHAAFTEMRARGANITDLTVLVVAADDGVMPQTIEAINHAKAAETPIIVAVNKIDKPGANPDHVTEQLTEYGLIPEDWGGDTIYVPVSAKFNKNIDELLDMILLQSEVMELKANPDQNAAGTVIEARLDHGRGSVATLLVQQGTLHVGDPIVVGDTYGRVRTMTNENGREMKKATPSTPVEITGLNDVPQAGDKFVVFDDEKTARAAGEERAKRAADRQRQQTSHVTLDNLFATMKQGEIKSLPVIIKADVQGSVEALAQSLQKIDVDGVQVDIIHKAVGAISESDVKLAEASNAIIVGFNVRPTPIAKNESEANNIDIRLHRVIYNAIEEIEDAMKGMLEPVFEEEVIGQVEVRQIYKASKIGTIAGGMVTSGRITRNSKVRLIRDGVVVYEGELGSLKRFKDDAKEVKAGYECGLTIENYNDIKEMDVIEAYEMKEVPVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKQ3</accession>
    <name>H4GKQ3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">2-oxo-hept-3-ene-1,7-dioate hydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_4961</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122635.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4961"/>
    <dbReference type="EnsemblBacteria" id="EHS84973">
        <property type="protein sequence ID" value="EHS84973"/>
        <property type="gene ID" value="PS3_4961"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1624"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="SSF56529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS84973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="269" mass="29653" checksum="40D7247FA4138202" modified="2012-04-18" version="1">MTELNARQEEFAQALVNAYKHNTALNEEDWKDAVSDFDTAYAVQERVMTLKGQPTAGYKVSLTSEETQKMFDTDAPLYGQQVAERFLKGPADVNVDHMNEPLLEVELGFRAKETLSPDDSLEDLLHKTTVCGTVELPDARFVNWFPALGKYNALSDCAVGGAVVYGEERDADQVFSSVDDAATVHVDLYHDGEKVSEGDSSEVLDNPLNSLQWLVKKLAEQGKVFARGQLVSSGTFLLPPHLTAGKWEAKFNHDFGTVTVDAHPTVSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKU3</accession>
    <name>H4GKU3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_8363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122675.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8363"/>
    <dbReference type="EnsemblBacteria" id="EHS85013">
        <property type="protein sequence ID" value="EHS85013"/>
        <property type="gene ID" value="PS3_8363"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1671"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="175" mass="20283" checksum="B641EC5352AFF5D1" modified="2012-04-18" version="1">MHLEQAHLNDLERVIEILKDGRNQLAERGIDQWQGDYPNRDHIKEDIENGYAYLVHSDDHETVGAFAMVPAPDQTYDALDGQWEIETDQYVVIHRVAIHSEHAGHGYASGLFVALIDYIKEQRPEILSIRIDTHEDNAPMQHLIEKYGFHRVGTLHGVYRPNEISFVYENIRENN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKV0</accession>
    <name>H4GKV0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative threonine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_12889</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122682.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12889"/>
    <dbReference type="EnsemblBacteria" id="EHS85020">
        <property type="protein sequence ID" value="EHS85020"/>
        <property type="gene ID" value="PS3_12889"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1679"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029144">
        <property type="entry name" value="Thr_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037158">
        <property type="entry name" value="Thr_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14821">
        <property type="entry name" value="Thr_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thr_synth_N" evidence="6">
        <location>
            <begin position="2"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="PALP" evidence="5">
        <location>
            <begin position="102"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR604450-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="496" mass="55398" checksum="C522BEAC85C991BD" modified="2012-04-18" version="1">MQYRSTRGTTDQSLSSSQAVIQGLANDGGLFVPVDFPKPNINLTEFVKLTYQEQARQVLGWFFDDYSAEQLDQIVAAAYNDQWDDQTITPVSDDHAGQYYLELFHGPTLAFKDVALQTLPHLMTNAIKLEKVDRKIVILTATSGDTGTASMCGFQDVDGTQVIVFYPHNGVAPIQLKQMFTQPGDNLNIVAVEGNFDDAQTYVKQIFNDPDFREQLLIHHDQFSSANSMNIGRLIPQITYYFNAYTQLVQREVIQVGDKVNFSVPTGNFGDILAGYYAKKLGLPINKLICASDSNNVLTDFFKTGEYDKRRDFNVTISPSMDILVSSNLERLLFDAADQDYELISQLMDQLTTEGHYQLPAKVYDRLSDFAAGFASEDEIRAEIKRVFTADHYALDPHTAVGSYVAHQYQKESGDSTPMIVVATASPYKFPETVYQALTDHPVEEAGLPAIKQLEKYLQTTPPAGIQTVFGPDHRTETVVKPAEMKAKISDILDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKY1</accession>
    <name>H4GKY1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_19787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000076">
        <property type="protein sequence ID" value="EHS84833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122713.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000076.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19787"/>
    <dbReference type="EnsemblBacteria" id="EHS84833">
        <property type="protein sequence ID" value="EHS84833"/>
        <property type="gene ID" value="PS3_19787"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1723"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="393" mass="44595" checksum="F4CABE6781C27DCC" modified="2012-04-18" version="1">MSKYRVEIPQEAMENTEIPKSGPLVMRVHRHQLVIEPENLASIVPQIKMSWYILPAILVAILVFSQLYADRIQLVPLSGPASSLGNIITAFGGLCGIGAFIITLISEKIKRTGPAQQFSWRMLLPIAMVSGLILVFTVYALFWVLSQLFGGATFDIYTACIMIAISIAVINYIMINLSMTLSPGVITNLMTIMILGGVLFSMLTNGRKNWWRYNFSYLGTAQSGSSMQFNLTLIFTGILMATLVDYLFVNLKRRYHNWRIEISRWMLFGLAACLAGVGLFPNNREFHYLHDQISMWLVYLLLILIIILRWILPMISNNFLRLSYLMGAVMAAEYIAFKFVHYLSLTAFELLSFGLALSWTLLLFQNLEYLIQSDSEILDIDLVVIKHESDDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKZ3</accession>
    <name>H4GKZ3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Poly(Glycerol-phosphate) alpha-glucosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_14592</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122725.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14592"/>
    <dbReference type="EnsemblBacteria" id="EHS84759">
        <property type="protein sequence ID" value="EHS84759"/>
        <property type="gene ID" value="PS3_14592"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1736"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="327"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="508" mass="58591" checksum="55C7C70236E33E6B" modified="2012-04-18" version="1">MLFFLNDNLQTNKSGIEHAQIKRQHLFNKFNSPAKIVTRQYSNELHKITQAAGVEDQDFINLFDYFQETLMVEYHPKQIQELAIDPSWKRQADGVNYNYYAGKHRVMYVRRDKRDHHVLNQQYFDRFGKLVKVAWFDYRGFLSVDQLYDWSNQITVQNYYRPDGSLAIQTTKTLNKREKEQQVYHLVNYRGRDYQFTNFDDLTRFFYDELVTDPAIVGDQKVGLIVDRSYEMGWAVLNMHHRVSRWLQLHNNHLNNGDDIMNSPLNYNYTWGLNHAQDWDGIIALTPQQAADVAARFAKETRVLQIPGPIVPDEVLKAPRVPFEQRRPGLVVVVARLSAEKQQDQLLQVWPQIKAAVPTAELELWGYANDHFDDKLKELVDQLQIKGSVHFKGYTSDVAQVYDQAQVMVLPSRAEGLPLSLVEGQSHGVAIVANDIKYGPAAVVIDGQDGYLTQNGDGDGLAKAIIDLLSHPDKLRQFSARAYQDSERYSEANVMQLWKNLISIVEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL23</accession>
    <name>H4GL23_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Replicative DNA helicase</fullName>
            <ecNumber evidence="3 11">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PS3_12187</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the helicase family. DnaB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3 11"/>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122755.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12187"/>
    <dbReference type="EnsemblBacteria" id="EHS84789">
        <property type="protein sequence ID" value="EHS84789"/>
        <property type="gene ID" value="PS3_12187"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1774"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00984">
        <property type="entry name" value="DnaB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036185">
        <property type="entry name" value="DNA_heli_DnaB-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007692">
        <property type="entry name" value="DNA_helicase_DnaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007694">
        <property type="entry name" value="DNA_helicase_DnaB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007693">
        <property type="entry name" value="DNA_helicase_DnaB-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016136">
        <property type="entry name" value="DNA_helicase_N/primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00772">
        <property type="entry name" value="DnaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03796">
        <property type="entry name" value="DnaB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48024">
        <property type="entry name" value="SSF48024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00665">
        <property type="entry name" value="DnaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51199">
        <property type="entry name" value="SF4_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 11 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0639">Primosome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SF4 helicase" evidence="12">
        <location>
            <begin position="180"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS84789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="456" mass="50788" checksum="6EC6AFE3D66D18C5" modified="2012-04-18" version="1">MDNTAELTRTPPRNLQAEQAVLGSALLTKDAIVEAMEFVQPDDFYSRQHQILYAAMLHLNDQDQPIDVINLQNELTRTNQLDNVGGVQYLAELAAAVPTAANVAYYARIVRDKATARRLIETATKIASEGYEDRQDVTTMLDEAERSILSVAESNTQTDLQSINTLLGQSADHLQELAQQTTDVTGLPMGYQQLDRMTTGLHADELIILAARPAMGKTAFVLNIAQNVAAHSQERPTVAIFSLEMGAESLVNRMLCAEGNINANHLRTGNMNADEWEQFWVASQTLSEAKIFIDDTPGIKVAEIRAKCRRLAKREGNLGLIVIDYLQLIEGSNSESRQQEVSEISRQLKKLAKELSVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIVGFLYREDYYTREQGAEQQDETNSLSEVEVIIEKNRSGPRGTVKLLFNKAYNKFASIDYEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL24</accession>
    <name>H4GL24_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">50S ribosomal protein L9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rplI</name>
        <name evidence="10" type="ORF">PS3_3341</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the bacterial ribosomal protein bL9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122756.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3341"/>
    <dbReference type="EnsemblBacteria" id="EHS84790">
        <property type="protein sequence ID" value="EHS84790"/>
        <property type="gene ID" value="PS3_3341"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1775"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.430.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00503">
        <property type="entry name" value="Ribosomal_L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000244">
        <property type="entry name" value="Ribosomal_L9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_L9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020594">
        <property type="entry name" value="Ribosomal_L9_bac/chp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020069">
        <property type="entry name" value="Ribosomal_L9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036791">
        <property type="entry name" value="Ribosomal_L9_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020070">
        <property type="entry name" value="Ribosomal_L9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036935">
        <property type="entry name" value="Ribosomal_L9_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21368">
        <property type="entry name" value="PTHR21368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03948">
        <property type="entry name" value="Ribosomal_L9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01281">
        <property type="entry name" value="Ribosomal_L9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55653">
        <property type="entry name" value="SSF55653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="SSF55658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00158">
        <property type="entry name" value="L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00651">
        <property type="entry name" value="RIBOSOMAL_L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RIBOSOMAL_L9" evidence="9">
        <location>
            <begin position="13"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS84790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="150" mass="16540" checksum="A31CB3942FED8222" modified="2012-04-18" version="1">MKVIFTEDVKGRGKRGEVKEVSDGYAQNFLIPRGKAKAANKAAMSELAGQKRSEEKHAAEELAEAKKLKKVLEDDKTVVELQGKAGSDGRMFGSVSTKQIAAALEKQYQIKLDKRKMELAAPIKSLGYVNVPVKLHHEVEATLRVHIAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL26</accession>
    <name>H4GL26_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_14479</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122758.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS84656">
        <property type="protein sequence ID" value="EHS84656"/>
        <property type="gene ID" value="PS3_14479"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1778"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="203" mass="21793" checksum="BE680D7DAC692E4B" modified="2012-04-18" version="1">MIILVVIAAIVLILGIFYLVNSWQQYRQWKLATFIVVLSLAGVIYGTINLPYWHQKTSSSASQSASASSLMSSSSLLSSSTTANQSAKASYILGQLQKNFTKLGTVSYDSKTKTYTITPSGDDITNALSALESDTSYADQIGWPNLTSNLQKTSQSIQKSIGDGYTLQLMSPNDNSKALLAVKDGQVTYNIVDTSSSTSSSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL34</accession>
    <name>H4GL34_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribonuclease Z</fullName>
            <shortName evidence="8">RNase Z</shortName>
            <ecNumber evidence="8">3.1.26.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">tRNA 3 endonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNase Z</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnz</name>
        <name evidence="11" type="ORF">PS3_14041</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RNase Z family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122766.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14041"/>
    <dbReference type="EnsemblBacteria" id="EHS84664">
        <property type="protein sequence ID" value="EHS84664"/>
        <property type="gene ID" value="PS3_14041"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1788"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07717">
        <property type="entry name" value="RNaseZ_ZiPD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01818">
        <property type="entry name" value="RNase_Z_BN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013471">
        <property type="entry name" value="RNase_Z/BN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02651">
        <property type="entry name" value="RNase_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lactamase_B" evidence="9">
        <location>
            <begin position="29"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="Lactamase_B" evidence="10">
        <location>
            <begin position="202"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS84664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="308" mass="34400" checksum="B07C60D314C963DC" modified="2012-04-18" version="1">MQLEFLGTGAGSPSKQRNVSSLALKLLEERNAVWLFDVGEATQHQILHTTIRPRKIEKIFITHLHGDHIFGLPGLLSSRSFQGGDEPLTIYGPVGIKTFVETALQISETRLSYPIEYVEIKQDGEIFKDQTFQVEARRMDHKIACFGYRIVEADHPGELQVEKLRELAIPAGPIYGQIKAGQTVTLPDGRQINGQDFIGAPQKGRIVAIIPDTRVNENSVKLAQNADVLVHEGTFAKNEQRMAHNYYHSTSAQAATVAKQAHVGRLLLNHISARYVGKAANQLAYQVQNIFPNTRVVKDFEEIEIPFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL42</accession>
    <name>H4GL42_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_21906</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122774.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21906"/>
    <dbReference type="EnsemblBacteria" id="EHS84672">
        <property type="protein sequence ID" value="EHS84672"/>
        <property type="gene ID" value="PS3_21906"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1797"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS84672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="297" mass="34595" checksum="2FF1D0557699DA84" modified="2012-04-18" version="1">MNSDQQIQFAKAQQALDQQQYSEAARILALLNDEVDLFVINHSLVKALYEIGQYRQAWFLINEHLADYQQSAELTALAVQVALANHQMINARKLALASRHDDRLLRQIDQAEDKMRETMQQSLQGIQRRFIHLGDQAFNGQQERFHAADQLPLNEYVQAAKYLVRDPFVRPLIRASIIQNLQQLQVKEIITIYWIDDQEYSVDLAKLKPVDEYPIVIEVQKLLSDRYGQSDPVTLQTYLQEFYLQLTLLFPRIEETIVDAQAWYVALCAYSQTTNEPAIQAAKEWQNRLTKLINRMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL49</accession>
    <name>H4GL49_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ComE operon protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_7302</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122781.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7302"/>
    <dbReference type="EnsemblBacteria" id="EHS84679">
        <property type="protein sequence ID" value="EHS84679"/>
        <property type="gene ID" value="PS3_7302"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1804"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004132">
        <property type="term" value="F:dCMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01286">
        <property type="entry name" value="deoxycytidylate_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013404">
        <property type="entry name" value="Competence_ComEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016473">
        <property type="entry name" value="dCMP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015517">
        <property type="entry name" value="dCMP_deaminase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035105">
        <property type="entry name" value="Deoxycytidylate_deaminase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11086">
        <property type="entry name" value="PTHR11086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006019">
        <property type="entry name" value="dCMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02571">
        <property type="entry name" value="ComEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="7">
        <location>
            <begin position="6"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR006019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="160" mass="18027" checksum="72D506812F5D1D76" modified="2012-04-18" version="1">MTQRIGWDQYFMLQAALLSSRSTCKRLSVGAVLVRDRRIIGSGYNGAVSGDEHCIDSECYLRDGHCVRTIHAEMNALLQCAKFGTSTDGAVVYVTDFPCLQCTKSLLQAGITQINYLRNYHNDEYAMELIKLKHVKLQQIKLSADTIDQVNLKQYLDSDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL50</accession>
    <name>H4GL50_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Competence protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_9744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122782.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9744"/>
    <dbReference type="EnsemblBacteria" id="EHS84680">
        <property type="protein sequence ID" value="EHS84680"/>
        <property type="gene ID" value="PS3_9744"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1805"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004509">
        <property type="entry name" value="Competence_ComEA_HhH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019554">
        <property type="entry name" value="Soluble_ligand-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10531">
        <property type="entry name" value="SLBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00426">
        <property type="entry name" value="TIGR00426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH1" evidence="1">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="1">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="211" mass="22728" checksum="5474DA5A3606366C" modified="2012-04-18" version="1">MDIEELRERLIDLWETQRTKLILGAGVLVCIGLFCWHQKPTPVSTNSPTMMNSTVSSRVATSTTTQNGRVSIDIKGAVAHPGVYTLPKQARVKEAIEQAGGSLPTADLNQVNLAKQLTDQQVIYIPLVGEKIAGANNIAAGSSTEGDQPIVNLNTATKEQLQTITGIGEKKADKILAYRQEHGEFKSVEELKSVDGFGDKTVAKLQSSLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL59</accession>
    <name>H4GL59_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ArsC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_8353</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ArsC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122791.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8353"/>
    <dbReference type="EnsemblBacteria" id="EHS84689">
        <property type="protein sequence ID" value="EHS84689"/>
        <property type="gene ID" value="PS3_8353"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1818"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006660">
        <property type="entry name" value="Arsenate_reductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30041">
        <property type="entry name" value="PTHR30041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03960">
        <property type="entry name" value="ArsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51353">
        <property type="entry name" value="ARSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="146" mass="16633" checksum="ACED475BB8D79FC0" modified="2012-04-18" version="1">MVTLFFHTNDPSKRKAVQWLTQHGITVTQRNIEKEPLTQAEVLDLLAISMDGTEDLISKRSRDTKALKMDSENVTINQLTAAVATNPHILRNPIIFNENKLVTGFDQEKMGVFIPKRQRRLELNRLLGKLTPAMGPQPQVEYARIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL67</accession>
    <name>H4GL67_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ADP-ribose pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_5746</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122799.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5746"/>
    <dbReference type="EnsemblBacteria" id="EHS84697">
        <property type="protein sequence ID" value="EHS84697"/>
        <property type="gene ID" value="PS3_5746"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1828"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="39"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="185" mass="20972" checksum="3E02CFAF65751EA8" modified="2012-04-18" version="1">MSFKERVTDVKSVYQGNVIEVELQTVETPNGHQATRDVVRHAPAIAILAITNDHKMILERQWRNPVNQVTIEIPAGKLDERDHGDDFLAAKRELNEETRYQAEKLVKINEDFSSPGFTDEKLSFYLATGLSAVDEALPQDADEQIELIYLSLPEALAQIRQGKINDLKTIMAIYFWQSMVGEANE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL68</accession>
    <name>H4GL68_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Isoleucyl-tRNA synthetase</fullName>
            <shortName evidence="11">IleRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ileS</name>
        <name evidence="17" type="ORF">PS3_5394</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="11"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122800.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5394"/>
    <dbReference type="EnsemblBacteria" id="EHS84698">
        <property type="protein sequence ID" value="EHS84698"/>
        <property type="gene ID" value="PS3_5394"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1830"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07960">
        <property type="entry name" value="Anticodon_Ia_Ile_BEm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02002">
        <property type="entry name" value="Ile_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033708">
        <property type="entry name" value="Anticodon_Ile_BEm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023585">
        <property type="entry name" value="Ile-tRNA-ligase_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010663">
        <property type="entry name" value="Znf_FPG/IleRS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06827">
        <property type="entry name" value="zf-FPG_IleRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 11 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA-synt_1" evidence="14">
        <location>
            <begin position="29"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_1" evidence="16">
        <location>
            <begin position="686"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="zf-FPG_IleRS" evidence="15">
        <location>
            <begin position="894"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="11">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="11">
        <location>
            <begin position="603"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="562"/>
        </location>
        <ligand>
            <name>L-isoleucyl-5'-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:178002"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="606"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="897"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="900"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="917"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="920"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF06827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EHS84698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="939" mass="107225" checksum="1869FED781BC2AD7" modified="2012-04-18" version="1">MKVKDTLNLGKTKFPMRGHLPEREGQREKLWEVNKVYEQRQKLNEGKPTFVLHDGPPYANGPIHIGHAMNKISKDFIVRYKSMTGYRAPYVPGWDTHGLPIEQQLTKEGYDRKKMSTTDFRDLCLEYAKKQIDIQRKGFKRLGVSGEWDNPYVTYAHEYEAAQIKVFGALAEKGLLYRGKKPVYWSWSSESALAEAEVDYKDITSQSAFYGERVKDGKGVLDNDTYFLVWTTTPWTIPASEGIVVGADFDYSLFTHDDDPKKYVVASDLVEKLSEKFGWHNVQTLKTYKGAEFEYMTAEHAFSSVDPFYKDREVLVMLGDFVTTSDGTGLVHTAPGYGDDDYQVGIKYGLPVFAPVNDQGYQTEEAGPDFAGVFYQDSEAIALKKLEDADLLVLQEDVTHSYPFDWRTKKPIIFRATDQWFVSIDKMRDDILKAVDEVTYYPAWGKTRLRNMLRDRGDWVISRQRVWGVPLPIFYAEDGTPIMTKETIDHVSDLFRQYGSNVWFDRDAKDLLPEGFTSEHSPNGKVTKETDIMDVWFDSGSSHQGVLAERDYLTYPSDMYLEGSDQYRGWFNSSLITSVVVSGHAPYKSVLSQGFTLDGEGKKMSKSVGNVIDPNEVVEKMGAEIIRLWVMSADTSADVRVSMGTLQQMSEAYRKLRNTFRFLLANTSDFDPKTNRVDYADLSSVDQYMAVKFNRFLKHMREYMDQYDFLDSYKLLINFVNNDLSAFYMNVAKDVLYIDGEDSQTRRSMQTVFYDILVGMTKLLTPFLPHTTEEVWEYLPEPEEYVQLTEIPEAVSFDQEDELLATWDAFMELRSHVLKALEEARDAKLIGRSLEAEADLYVTPSQQTTLDNLNADAALMFGVSNLRVHSVDETPADAEVFNDKAAVKVSVAQGETCERCRMVKEDVGSDPDFPQLCARCAQLVRENYPEAVTEGFESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL80</accession>
    <name>H4GL80_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 7">Transcriptional regulator MraZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mraZ</name>
        <name evidence="10" type="ORF">PS3_4117</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Forms oligomers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MraZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122812.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4117"/>
    <dbReference type="EnsemblBacteria" id="EHS84710">
        <property type="protein sequence ID" value="EHS84710"/>
        <property type="gene ID" value="PS3_4117"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1844"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009295">
        <property type="term" value="C:nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16321">
        <property type="entry name" value="MraZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16320">
        <property type="entry name" value="MraZ_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01008">
        <property type="entry name" value="MraZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003444">
        <property type="entry name" value="MraZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035644">
        <property type="entry name" value="MraZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020603">
        <property type="entry name" value="MraZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035642">
        <property type="entry name" value="MraZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038619">
        <property type="entry name" value="MraZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34701">
        <property type="entry name" value="PTHR34701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02381">
        <property type="entry name" value="MraZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="SSF89447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00242">
        <property type="entry name" value="TIGR00242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="9">
        <location>
            <begin position="5"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="SpoVT-AbrB" evidence="9">
        <location>
            <begin position="76"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS84710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="143" mass="16309" checksum="DBB3883D3A4F6EC3" modified="2012-04-18" version="1">MFMGEFEHSIDNKGRLIIPAKFRNQVGSTCVVTRGMDHSLSVYTMEEWDKVKQRLAQLPSTKGNARKFVRFIFSAATECEFDKQGRINIPQNLLTHAEIDKKCAIIGVSSHFEIWDADNWSTYQADAAEDFDDIAEEIGDLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLA8</accession>
    <name>H4GLA8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutamine ABC transporter ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_11033</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122840.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11033"/>
    <dbReference type="EnsemblBacteria" id="EHS84607">
        <property type="protein sequence ID" value="EHS84607"/>
        <property type="gene ID" value="PS3_11033"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1885"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS84607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="214" mass="23220" checksum="556958FE19A7A5D8" modified="2012-04-18" version="1">MLEVKNLKKKFGTRQILDGVNFEVQDGEILSIVGPSGAGKTTLLRCVTGLESADEGEFLIDGQAFDPEGTDTSDAVIGVVFQDFNLFPHLTVMQNITLAPTMVLKEDRQVAQAAGEKLLAQLDLGGKGDLYPYQLSGGQKQRVAIARALAMNPKILCYDEPTSALDPNLRDSVANLILSLKQQGMTQLVVTHDMEFAQKIADDVLEVSALDQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLB6</accession>
    <name>H4GLB6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cysteine desulfurase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_9195</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122848.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9195"/>
    <dbReference type="EnsemblBacteria" id="EHS84615">
        <property type="protein sequence ID" value="EHS84615"/>
        <property type="gene ID" value="PS3_9195"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1894"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_5" evidence="4">
        <location>
            <begin position="2"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS84615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="381" mass="41773" checksum="812CD8C324F9A11C" modified="2012-04-18" version="1">MIYFDNSATTKVSAEVLESYTKVSEQIWGNPSSLHQLGEVASKLLFQARQQIAELFGVLPGEIIFTSGGSEGDNWVIKGTALAKRELGRHIITSSVEHAAVRNAMHQLEDLGYEVTYLPVDKQGRVNPADLKAAIRPDTILVSIMAVNNEIGTVQPIQEIGEILQDYPKIHFMVDAVQAIAKGMDDQWFSDRVDFVVFSGHKYHAPRGTGFVYAKAGRQIAPLINGGGQEQNLRSGTENTPGDVAMSRAIRLAKEREDDTVKREREIKQAIIDHVSQFEHVQVFSGNDAGFAPHILCFAIVGVRGETVVHAFEEHDIYISTTSACSSKKTETSTLSAMGVDPHAAESAVRVSLGDQNTLADAEEFNRVFDELYAGFAKIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLB8</accession>
    <name>H4GLB8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">30S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpsD</name>
        <name evidence="11" type="ORF">PS3_7503</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="6">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122850.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7503"/>
    <dbReference type="EnsemblBacteria" id="EHS84617">
        <property type="protein sequence ID" value="EHS84617"/>
        <property type="gene ID" value="PS3_7503"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1896"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_B">
        <property type="entry name" value="Ribosomal_S4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_S4/S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_S4/S9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005709">
        <property type="entry name" value="Ribosomal_S4_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_S4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="PTHR11831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF4">
        <property type="entry name" value="PTHR11831:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01017">
        <property type="entry name" value="rpsD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_S4" evidence="10">
        <location>
            <begin position="3"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="S4" evidence="9">
        <location>
            <begin position="93"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS84617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="201" mass="22943" checksum="8C69EB9B8CB877AA" modified="2012-04-18" version="1">MSRYTGPSWRISRRLGVSLSGTGKELARRPYAPGDHGNDHRGKLSEYGTQLREKQKLRYMYGLTERQFSNLFVKAGKIKAGTHGTNFMVLLERRLDNMVYRLGLATTRRQARQLVNHGHITVDGKRVDIPSYEVKVGQVIAVREKSKNLDIIKNAVEAVVARPSYVEFDADKLEGHLTRLPQREDMNADIDEALIVEFYNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHR1</accession>
    <name>H4GHR1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acid sugar phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_16589</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the dephosphorylation of 2-6 carbon acid sugars in vitro.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the HAD-like hydrolase superfamily. NagD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.-" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000003">
        <property type="protein sequence ID" value="EHS87556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121595.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000003.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16589"/>
    <dbReference type="EnsemblBacteria" id="EHS87556">
        <property type="protein sequence ID" value="EHS87556"/>
        <property type="gene ID" value="PS3_16589"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.23"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006354">
        <property type="entry name" value="HAD-SF_hydro_IIA_hyp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000915">
        <property type="entry name" value="PGP-type_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01457">
        <property type="entry name" value="HAD-SF-IIA-hyp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="261" mass="29004" checksum="836D3C8E5835DB20" modified="2012-04-18" version="1">MKHYDTYFIDLDGTIYQGKNRIPAAATFIQRLKDHGKRILYLTNNSTRTPEEVAQVLTKDHQIPTDPKDVYTSALATADYVKSIASTGQERVNVIGEWGLQKALLDRGFYLTTYHPDFVVVGMDMNATYEDFATSVLNIQRGAKFIGTNPDTNLPNERGMLPGAGSLVALVHYATQAVEPIVVGKPHALIMEMALQYTGKQVDQVLMVGDNFHTDIQAGQAVGMDTLLVYTGVSKRSEVAKESQQPTYQINSLDEWDEENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHR8</accession>
    <name>H4GHR8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">PTS system mannose-specific IID component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_22298</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000004">
        <property type="protein sequence ID" value="EHS87548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121602.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000004.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_22298"/>
    <dbReference type="EnsemblBacteria" id="EHS87548">
        <property type="protein sequence ID" value="EHS87548"/>
        <property type="gene ID" value="PS3_22298"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.31"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004704">
        <property type="entry name" value="PTS_IID_man"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03613">
        <property type="entry name" value="EIID-AGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51108">
        <property type="entry name" value="PTS_EIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="307" mass="33963" checksum="0023185A3BBD8A77" modified="2012-04-18" version="1">MADNKIKLTKRDRWDVCWRSTFLQGSWNYERMQNGGYMYAMSPALKKLYKTKEDRAAAAKRHLEFFNTHPYVASPIIGVTLALEEDKANGVDVDDTTMSGVKVGMMGPLAGVGDPVFWYTARPIIAGLGASLAIAGNILGPIIFFVVWNIMRIAFMWYTQEFGYRAGTQITDDTNGLSLQTVSRGASMLGMFILGTLIERWVTITFTPVVSRTPVSKGGYIEWDKLPHNAHAIQQALQQWDMGNGLALSSTKVTTLQDNLDSLIPGLAALLLTFLCMWLLKKKVSPIWIIIGIFVAGIVLHVIGLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHS5</accession>
    <name>H4GHS5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA topoisomerase 1</fullName>
            <ecNumber evidence="10">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">DNA topoisomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">topA</name>
        <name evidence="13" type="ORF">PS3_14585</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="10"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14585"/>
    <dbReference type="EnsemblBacteria" id="EHS87481">
        <property type="protein sequence ID" value="EHS87481"/>
        <property type="gene ID" value="PS3_14585"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.44"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03363">
        <property type="entry name" value="TOPRIM_TopoIA_TopoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00952">
        <property type="entry name" value="Topoisom_1_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013498">
        <property type="entry name" value="Topo_IA_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005733">
        <property type="entry name" value="TopoI_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028612">
        <property type="entry name" value="Topoisom_1_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034149">
        <property type="entry name" value="TOPRIM_TopoI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42785">
        <property type="entry name" value="PTHR42785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01396">
        <property type="entry name" value="zf-C4_Topoisom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="SSF56712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01051">
        <property type="entry name" value="topA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPOISOMERASE_I_PROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="12">
        <location>
            <begin position="22"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="10">
        <location>
            <begin position="182"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="10">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="320"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="10">
        <location>
            <position position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS87481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="706" mass="79990" checksum="A1A0CF638F5E0C24" modified="2012-04-18" version="1">MATKTTTKKSTKKKTTRKAPQKKLVIVESPSKAKTIEKYLGRTYKVVASVGHVRDLPKSRMGVDIENDYQPEYISIRGKGSVIKELKKDAKNAKAVYLASDPDREGEAIAWHIATLLKDSNENLNRVTFNEITKDAVKESFNEPRQINMNLVDAQQARRVLDRLVGYSISPILWKKVKKGLSAGRVQSVALNLIIKRENEIKNFKPEEYWTLDAEFKKGREKFAASFYGIDGQKTKLPNNEAVQQVLGQLDRKAPFLIENVERKERRRAPQPPYTTSTLQQDANRRLNFRTRKTMMAAQQLYEGINIGQGAAVGLITYMRTDSTRISELAKHQAAEFIHDQYGAEYAATKPRKGKLPEGAQDAHEAIRPTSVLRTPASIKQYLTNDQYKLYNLIWCRFVASQMTAQVTDTMTVTITQGQVNFRANGSKVKFPGFTKVYKRGDEKDNLLPDLAVGDQVTMVKDDPAQHFTQPPARYTEAALVKALEENGVGRPSTYAPTLDTIQRRYYVRLVSRHFEPTELGEIVDTIISEQFPEIVNVKFTADVEQNLDAVEAGKENWVKVIDDFYQPFAKEVVQAEDQVAKIEMRDELAGQDCDICGAPMVIKMGRYGKFYACSRFPNCRNTKAIVKDTGITCPKCGQGTVVERKSKRNRTFYGCSRYPDCDFVSWDRPIDRKCPKDGHFLVEKKVKGGKQVVCPNGDYQEEVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHU7</accession>
    <name>H4GHU7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Segregation and condensation protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">scpB</name>
        <name evidence="5" type="ORF">PS3_10531</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Participates in chromosomal partition during cell division. May act via the formation of a condensin-like complex containing Smc and ScpA that pull DNA away from mid-cell into both cell halves.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Homodimerization may be required to stabilize the binding of ScpA to the Smc head domains. Component of a cohesin-like complex composed of ScpA, ScpB and the Smc homodimer, in which ScpA and ScpB bind to the head domain of Smc. The presence of the three proteins is required for the association of the complex with DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Associated with two foci at the outer edges of the nucleoid region in young cells, and at four foci within both cell halves in older cells.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ScpB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10531"/>
    <dbReference type="EnsemblBacteria" id="EHS87503">
        <property type="protein sequence ID" value="EHS87503"/>
        <property type="gene ID" value="PS3_10531"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.68"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051304">
        <property type="term" value="P:chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01804">
        <property type="entry name" value="ScpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005234">
        <property type="entry name" value="ScpB_csome_segregation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34298">
        <property type="entry name" value="PTHR34298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04079">
        <property type="entry name" value="SMC_ScpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019345">
        <property type="entry name" value="ScpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00281">
        <property type="entry name" value="TIGR00281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 4" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="204" mass="22465" checksum="F0B20F75AD495183" modified="2012-04-18" version="1">MNERQSINMDNQAQIEGLLFISGDEGISVAELASATGFMKPAVLEILNQLASQYASNEKSALTLLHSGETYRLATKAALAPVMKRYFETPLTVPLTPAILEVLAIIAYKQPITRIEIDEIRGVQSSGSIQKLIMRGLIEAKGRLEVPGRPYQYATTAAFLDYFGLDSLEQLPPLDDELDEASLDGDIFLRALQSRQAMNEESED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHV0</accession>
    <name>H4GHV0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA polymerase III subunit alpha</fullName>
            <ecNumber evidence="3">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PS3_4913</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase type-C family. DnaE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121634.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4913"/>
    <dbReference type="EnsemblBacteria" id="EHS87506">
        <property type="protein sequence ID" value="EHS87506"/>
        <property type="gene ID" value="PS3_4913"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.74"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011708">
        <property type="entry name" value="DNA_pol3_alpha_NTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041931">
        <property type="entry name" value="DNA_pol3_alpha_thumb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040982">
        <property type="entry name" value="DNA_pol3_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029460">
        <property type="entry name" value="DNAPol_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004805">
        <property type="entry name" value="PolC_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32294">
        <property type="entry name" value="PTHR32294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07733">
        <property type="entry name" value="DNA_pol3_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17657">
        <property type="entry name" value="DNA_pol3_finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14579">
        <property type="entry name" value="HHH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00594">
        <property type="entry name" value="polc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 13" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="POLIIIAc" evidence="12">
        <location>
            <begin position="3"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS87506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="1106" mass="125379" checksum="AB4715065EF1B2D2" modified="2012-04-18" version="1">MFVPLQIKSSYSLLQSTIQPRELVKTAKERGYQAIAITDEQVMYGVIEFYQAAQQYGIKPLIGLEMTVLFGQLATQQVKLLFYAQSYQGYQSLMQLSTLRMTKQNDLTWQDVQTHLTDVIVIVAPQLMAIDAVQIALLQSIRQVVTDRLYMGINLNLDPIRRQVIIDLAQQIRTPVVACEAVEYLESNQYFASQVLRSIGTKHVIEDPLNGVQHLGTHELKPMVDVEQAYQAAGLADAARKTERVARRCQLELNFQAPILPQFPTPNQEDAYTYLKTLCQQGLQNRPIAKGHAVSEYQARLDHELSVIHDMGFDNYFLIIWDVMAFAHQHQITTGPGRGSAAGSLVAYALAITDVDPIQYQLLFERFLNPERQQMPDIDLDLPDNRREEVLTYVHQKYGHQRVAQIITLGTLAARQAVNSVGQVLGVPKYQLAEVQQVVRRQTPTNQKYTLKQVVQASQALQNMMQDDPLIKLLIEVAEDLEGLPRNVSTHAAGLVLSEDPLIDTVPLQNGSSEAGLLMTQYPKDPVETLGLLKMDFLGLRNLSIMDEAMRLIHQTNPDFSLNQVNLTDPLTMKLFQTGETDGVFQFESNGIRRVLQQLQPDSFEDIVAVNALYRPGPMNNIDHFIARKHGQEATNYITPALEPILKNTYGILVYQEQVMQVAVAMAGFSLGQADSLRRAMSKKKAQEMERMRHSFVQGALQKGYEESVAQQVFDYMDQFANYGFNRSHAVAYSKMAFEMAYLKAHYPLAFYAALLSIEPNPEKEIEHFQSAKRMQVAIKNPSINQSDSDFTVENNRLRIGLGFIKGIRGDFVKAILTERQARGPFENLNDFINRLDERWQNEKQILPLIYVGAFDRLGYNRAEMVQALPGLIEGASKFQTLSLSDELAPVIERRPELNINERLAKESEYLGLYISGHPVNQYAKLQARLQSQVISHLKADQIVTLIVQINRVRVVKTKRDRREMAFLTVSDETGNLDVTVFPSQYLQFKTNLQNNQIVVINGKVEQRNQELQLIANRIALAQQVQAETTPKRWVLRITPGVSEQQIINLLNQLQREASGNYPVIFYDAVTNQAQQSRYHLDGENKVIDHLKELLGNENVILQEMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHV5</accession>
    <name>H4GHV5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_7154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121639.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7154"/>
    <dbReference type="EnsemblBacteria" id="EHS87511">
        <property type="protein sequence ID" value="EHS87511"/>
        <property type="gene ID" value="PS3_7154"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.80"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016429">
        <property type="term" value="F:tRNA (adenine-N1-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006901">
        <property type="entry name" value="TrmK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04816">
        <property type="entry name" value="TrmK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018637">
        <property type="entry name" value="TrmK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="239" mass="27014" checksum="FD59FC1C3A1E719B" modified="2012-04-18" version="1">MDALHLSQRLKLVADFVPQGARLADIGSDHAYLPAYLLLNQRIEFAIAGEVVKGPFENAQQEVLKHHLTGSLEARLADGLVAIEVADQIDVITICGMGGSLISQILDAHPEKLVGVKRLILQPNVGESELRTWLVQHHFEIMTERILTEDNQFYEIIVAEPTILPVAYNHRELTFGPELIMHQGPVFQAKWQGEIERLSQVIVQMQEAKQPPLRKIKEFQAQLQLIQEVLTDDNRNRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHX4</accession>
    <name>H4GHX4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Diacylglycerol kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PS3_6495</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial diacylglycerol kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121658.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6495"/>
    <dbReference type="EnsemblBacteria" id="EHS87530">
        <property type="protein sequence ID" value="EHS87530"/>
        <property type="gene ID" value="PS3_6495"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.100"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14265">
        <property type="entry name" value="UDPK_IM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000829">
        <property type="entry name" value="DAGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036945">
        <property type="entry name" value="DAGK_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033717">
        <property type="entry name" value="UDPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34299">
        <property type="entry name" value="PTHR34299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01219">
        <property type="entry name" value="DAGK_prokar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="127" mass="14405" checksum="90420EB1358AEE72" modified="2012-04-18" version="1">MDSNGKHQLNKNKTFRQAFHHAWDGIIAVVKHERNMRFHLLATIAVIIVACWFRLQVWEWLWILLAIFLVITGEMVNTIVEALVDMTVGHQYHPLAKLAKDMAAGLVLLLAGFAIIVGLLIFGNHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI12</accession>
    <name>H4GI12_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protease HtpX homolog</fullName>
            <ecNumber evidence="12">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">htpX</name>
        <name evidence="14" type="ORF">PS3_13383</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the peptidase M48B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="12"/>
    <dbReference type="EMBL" id="AICN01000007">
        <property type="protein sequence ID" value="EHS87463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121696.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000007.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13383"/>
    <dbReference type="EnsemblBacteria" id="EHS87463">
        <property type="protein sequence ID" value="EHS87463"/>
        <property type="gene ID" value="PS3_13383"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.146"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00188">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022919">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 12" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 12" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M48" evidence="13">
        <location>
            <begin position="79"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS87463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="297" mass="32495" checksum="CE1FB36B97D439DD" modified="2012-04-18" version="1">MLYQQIAQNKRRTIYVMAGFTLLVALIGAALGYLFAGTATGGVIIALIITLIYISVMVGQSTDVVMNMNHATEIHSADENPELWHVVEDMAMVAQVPMPRVFIIDDPSPNAFATGNNPQHAAVAATKGLLAMMNREELEGVMAHEMSHVKNYDIRLQTIALALAAAISLLVNFASNFWWFGGGDRDRERDGGGVIAILGSVLLIILAPLAASIAQMALSRNREYLADASAVELTRNPQGMISALRKLEDVQPMKAADPNSSALYISDPFKNQKHRSFSHLFDTHPPLEDRIVRLEKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI25</accession>
    <name>H4GI25_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphate-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PS3_17949</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in the system for phosphate transport across the cytoplasmic membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex PstSACB involved in phosphate import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 11">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the PstS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000011">
        <property type="protein sequence ID" value="EHS87435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17949"/>
    <dbReference type="EnsemblBacteria" id="EHS87435">
        <property type="protein sequence ID" value="EHS87435"/>
        <property type="gene ID" value="PS3_17949"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.164"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042301">
        <property type="term" value="F:phosphate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011862">
        <property type="entry name" value="Phos-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02136">
        <property type="entry name" value="ptsS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7 11" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_domain" evidence="13">
        <location>
            <begin position="29"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS87435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="297" mass="31980" checksum="B1B6D00A46FCC4A2" modified="2012-04-18" version="1">MHKQRWWLLALMIIILFVVGAYLAHQTNSKAKSAKITSVGSTALQPLIEAAVPGFQKQASTVNVVVQGGGSGTGLSQVASGAVEIGMSDVFAQQKSGIDASKLNDHIVAVTGIVPIVNPKLGVKNLSLTQLRAIFAGEITNWQEVGGPDEPITVINRASGSGTRMAFEQLVMQGSKTTQAQEQDSNGTVKQIVANVPGAISYVSVAYVNSQVKVLKVNGVSATYSNITQGYWPIWSYEHLYTIKHPSQATKAFIKYVQSPTVQNNLVKKSHYVSIHDMQVQQDANGKITKISEDHHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI35</accession>
    <name>H4GI35_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="9">1.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PS3_6367</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.-" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000012">
        <property type="protein sequence ID" value="EHS87427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121719.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000012.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6367"/>
    <dbReference type="EnsemblBacteria" id="EHS87427">
        <property type="protein sequence ID" value="EHS87427"/>
        <property type="gene ID" value="PS3_6367"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.175"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43148">
        <property type="entry name" value="PTHR43148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gp_dh_N" evidence="10">
        <location>
            <begin position="3"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="5">
        <location>
            <begin position="152"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="5">
        <location>
            <begin position="212"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="12"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="7">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="336" mass="36038" checksum="3958B4E72B395EC8" modified="2012-04-18" version="1">MTVKIGINGFGRIGRLAFRRIHELGAKDIEVVAINDLTTPAMLAYLLKYDSTHGAFPGEVSATETGIVVDGKEYPVYAERDAKNIPWVKNDGVQFVLECTGFYTSEEKASAHLEAGAERVLISAPAGNIKTVVPGVNLDTLTADDKIVSAASCTTNCLAPMAYFMNKNFGVKAGTMTTIHAFTSTQMILDGPRGSKMRNNRTASVNTIPHSTGAAKAIGLVVPELNGKLQGHAQRVGVVDGSLTELVTVLDKKVTVEEVNEAMKKATEGNEAFGYTEDEIVSSDIIGSTFGSVFDPSQTEVTTAGDTQLVKTVAWYDNEYGFTSNMIRTLLHFATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI55</accession>
    <name>H4GI55_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptide deformylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_6006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the polypeptide deformylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121739.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6006"/>
    <dbReference type="EnsemblBacteria" id="EHS87394">
        <property type="protein sequence ID" value="EHS87394"/>
        <property type="gene ID" value="PS3_6006"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.203"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042586">
        <property type="term" value="F:peptide deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00487">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023635">
        <property type="entry name" value="Peptide_deformylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036821">
        <property type="entry name" value="Peptide_deformylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10458">
        <property type="entry name" value="PTHR10458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01327">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004749">
        <property type="entry name" value="Pep_def"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01576">
        <property type="entry name" value="PDEFORMYLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56420">
        <property type="entry name" value="SSF56420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="136" mass="15154" checksum="830739DCA8F6D31F" modified="2012-04-18" version="1">MIKPICKDPQVLSQPSQPATPHDWPIAQDLLDTLVAHQKECVGLAANMIGINKQIIAVSLGPINIAMLNPQITVKEQPYQTQEGCLSLIGQRPTRRYQEITLVYLDQTGHQQKLSLHGWAAQIVQHEIDHCHGIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI72</accession>
    <name>H4GI72_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Replication initiation and membrane attachment protein DnaB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_7298</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121756.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7298"/>
    <dbReference type="EnsemblBacteria" id="EHS87411">
        <property type="protein sequence ID" value="EHS87411"/>
        <property type="gene ID" value="PS3_7298"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.226"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006343">
        <property type="entry name" value="DnaD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07261">
        <property type="entry name" value="DnaB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DnaB_2" evidence="2">
        <location>
            <begin position="319"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="454" mass="51317" checksum="A592EA1B99AD2559" modified="2012-04-18" version="1">MGNNLVRLQSQTGYVVSLSPDFGKFDQTTFLNFYQPLMSPTAMGLFYALQLNLSVHPVLTDRQPHQKLMVQLGAGIQAIDQGRQQLEALGLLKTFQQIDDLGEVLVYELHPTLSPDEFLADDLLSVLLLQMVGEQAFNQLSQRANQYYLKSSELAEVTTPFLDVYTIGKQALKQVDPVIETARQTNALVKSKSSKVSDPAFDWELLGQQLQLQGIDHQVMTDYHQVIMAEHLLYQLDEFQLAELMIKAVDVTNNQFDVDRFKQLVRQQGQASRQPSAQTSNSNEDLRDLSEVEKQLVQQVQAYSPLDYLTGLKNATNSGYVSNNERYTLERLVEAQTLPISAINVLLHYVIVEQGKASLNANLVDAIANTWTRKGVQSASDAIRALKDFRKQRQTKSRQSRRTSNGKHVKEQLPEWAKKADYQPRPAKKATDEGNLSNIENLKSKLRQQQNNQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI92</accession>
    <name>H4GI92_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GlnQ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_11417</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11417"/>
    <dbReference type="EnsemblBacteria" id="EHS87313">
        <property type="protein sequence ID" value="EHS87313"/>
        <property type="gene ID" value="PS3_11417"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.254"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015424">
        <property type="term" value="F:ABC-type amino acid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030679">
        <property type="entry name" value="ABC_ATPase_HisP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039085">
        <property type="entry name" value="ABC_ATPase_HisP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="245" mass="27313" checksum="C1F017DF78BAA0DA" modified="2012-04-18" version="1">MIEFKNVQKYYGDFHALKDINLKIDAGETVVLIGPSGSGKSSLIRTVNGLDPIRSGQLIVNGFDLGDKKTDVNKIRKDVGMVFQHFNLYNNKDVLENIMLAPRVVLKRPEEENKKLAMGLLEQVGLADKAHNMPAQLSGGQKQRVAIARSLAMKPKCLLFDEPTSALDPEMIDDVLDVMKEIAHRSDMTMLVVTHEMGFAKAVADRVIFMADGRILEDDSTENFFDGEPSNERARQFLSKINGHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIB5</accession>
    <name>H4GIB5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative hexulose-6-phosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_17767</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121799.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17767"/>
    <dbReference type="EnsemblBacteria" id="EHS87336">
        <property type="protein sequence ID" value="EHS87336"/>
        <property type="gene ID" value="PS3_17767"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.285"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04726">
        <property type="entry name" value="KGPDC_HPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041710">
        <property type="entry name" value="HPS/KGPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OMPdecase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="212" mass="23125" checksum="769DAF0153A9FFC4" modified="2012-04-18" version="1">MTKPMLQVALDTEDTATALNIVRQVGDVVDVVEAGTLLIYEEGLDAVRALRAVAPDKIILADTKTADAGGHVAEECKKAGADWMTCINAATVPTMASAQKEIEVQVELYEGWDNEERAQAWLDNGVDQVVYHQSRDAKFAGQTWGEKDLESVKKLINMGFKVSVTGGMKPDVIKLFEGVPVYTFIAGRAIHGADDPHAAALEFKSEIDKYWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GID4</accession>
    <name>H4GID4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histidine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Histidyl-tRNA synthetase</fullName>
            <shortName evidence="9">HisRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">hisS</name>
        <name evidence="11" type="ORF">PS3_4558</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-histidine + tRNA(His) = AMP + diphosphate + H(+) + L-histidyl-tRNA(His)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121818.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4558"/>
    <dbReference type="EnsemblBacteria" id="EHS87355">
        <property type="protein sequence ID" value="EHS87355"/>
        <property type="gene ID" value="PS3_4558"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.322"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="PTHR43707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="10">
        <location>
            <begin position="24"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="431" mass="49047" checksum="A6A6846BF9C41AA8" modified="2012-04-18" version="1">MAKAKSRYQKPKGTADILPGDSELWQKVEAVARETFHQYGYSEIRTPMFEEFNVYSRNVGETSDIVTKEMYDFDDKGGRHIALRPEGTAGVVRSYVENKLFGPEYPKPYKMFYIGPMFRYERPQSGRQRQFTQIGCEALNSESPQIDAEVILEALTFFKRLGINNLRVAINTLGDRESREAYHAALVNYLTPYKDELSADSQTRLEKNPLRVLDSKDEHDQAIVANAPSIMDSLNEYSANYFAQVKSLLDDFEIDYVVDPTMVRGLDYYTHTIFEIMSDSKAFGGGYVTVCGGGRYNGLIGQLGGPEEEGGIGYGLGFERLLLLLEEENPAFIPQPSLDLFFVSANQTGDQYAFKLMQKLREQGLAVDKDYQGIKLGKQIKEAERKNSRYFVVVGDREVETEELEIKSVTDNQRQRVQVKDLLDDPEKYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIH2</accession>
    <name>H4GIH2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Glucose-inhibited division protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mnmG</name>
        <name evidence="11" type="synonym">gidA</name>
        <name evidence="14" type="ORF">PS3_6941</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 11">NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9 11">Homodimer. Heterotetramer of two MnmE and two MnmG subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the MnmG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6941"/>
    <dbReference type="EnsemblBacteria" id="EHS87293">
        <property type="protein sequence ID" value="EHS87293"/>
        <property type="gene ID" value="PS3_6941"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.373"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00129">
        <property type="entry name" value="MnmG_GidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004416">
        <property type="entry name" value="MnmG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020595">
        <property type="entry name" value="MnmG-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026904">
        <property type="entry name" value="MnmG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044920">
        <property type="entry name" value="MnmG_C_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="PTHR11806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF0">
        <property type="entry name" value="PTHR11806:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13932">
        <property type="entry name" value="GIDA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00136">
        <property type="entry name" value="gidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01280">
        <property type="entry name" value="GIDA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01281">
        <property type="entry name" value="GIDA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 11" id="KW-0520">NAD</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="GIDA" evidence="12">
        <location>
            <begin position="23"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="GIDA_C" evidence="13">
        <location>
            <begin position="419"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="28"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="291"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS87293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="643" mass="71920" checksum="07BEB558CD29007B" modified="2012-04-18" version="1">MFREIMEKKMEAKVIQYQGPSYDVIVVGAGHAGSEAALAAARMGQKTLLITISLEMVAFMPCNPSLGGPAKGVVVREIDALGGEMGHNIDKTYIQMRMLNTGKGPAVRALRAQADKHAYHRQMKWTIEQEPNLTLRQGLVDELIVEDGVVKGVVTNTGARYLAKAVVLTVGTAARGKIIIGELQYESGPNNSKPAMKLTASLKELGFDIQRFKTGTPPRVNGNTIDYQVTEEQPGNPEPHHFSFDTPDTAYLPLKDQISSWLTYTNPTTHQILRDNLDRAPLFSGLIEGVGPRYCPSIEDKIVRFADKDRHQVFLEPEGRDTDEFYLDGLSTSMPEEIQQKMIHSIKGLETAEMMRPGYAIEYDVVAPYQLHPTLETKLIEGLYTAGQTNGTSGYEEAAGQGLIAGINAALKIQGKEPLVLKRSESYIGVMIDDLVTKGTKEPYRLLTSRAEYRLLIRHDNADLRLMKYGYRIGLVSDERFAHLKAKQTHIQDEINRLNNYRIKPGQAVDAFIKANGDNPLKDAIVAADFLKRPYVDYQTLQQFVPALEQPLSRHEQEQVEIQIKYDGYIKKEEARIERMKRMEEKRIPATIDYNQIEGLATEGRQKLAKIRPETLAQASRISGVNPADLAILSVYLRQGKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GII3</accession>
    <name>H4GII3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH tetR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_18391</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000018">
        <property type="protein sequence ID" value="EHS87278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87278">
        <property type="protein sequence ID" value="EHS87278"/>
        <property type="gene ID" value="PS3_18391"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.385"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="18"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="207" mass="23650" checksum="9A86BF54C52BFC6A" modified="2012-04-18" version="1">MKASSYTGGSYETCRKKARNRQLILTTTTEMIYQVGVAKTNLRQISKLSGLSVVTIYKYFTDKTDLINATISQMIEERFEQAESLIRTQDLPFIERIEKIPGPDKLNQELPEEIVQELIKTIADSPLLLKTLQERIKNFFQIIIIEGRKSNYIQTTASDQAIIDLFTLLIFRDNTGKAQPDPQRAADIIQIILYGLTNPGQQLTFHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>H4GII8</accession>
    <name>H4GII8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Extracellular protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_19326</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000019">
        <property type="protein sequence ID" value="EHS87276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19326"/>
    <dbReference type="EnsemblBacteria" id="EHS87276">
        <property type="protein sequence ID" value="EHS87276"/>
        <property type="gene ID" value="PS3_19326"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.391"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="134" mass="14401" checksum="517A3CEF97704679" modified="2012-04-18" version="1">MSDSASSYSAPLSSGSAVNDQNEDTNSLVIDDSETTEGTASETTTDTTSTTTDTSSSTTSDEEAAAKEWIAQKESSGSYTAQNGQYYGRYQLSLSYLNGDLSAENQEKVADAYVTNRYGSWVAAKAFWEANGWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIJ7</accession>
    <name>H4GIJ7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Thioredoxin reductase</fullName>
            <ecNumber evidence="8">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PS3_10189</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="9">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121881.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10189"/>
    <dbReference type="EnsemblBacteria" id="EHS87192">
        <property type="protein sequence ID" value="EHS87192"/>
        <property type="gene ID" value="PS3_10189"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.406"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 9" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 8" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="10">
        <location>
            <begin position="9"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="312" mass="33721" checksum="7F808C79F2CBD677" modified="2012-04-18" version="1">MVEATKTNYDVIVIGAGPGGMTTAMYASRADLDVLMLDRGIYGGNLNNTGTIENYTGFKTVQGPELAEQMYQGATQFGANYAYGSVTKVTVDKDGLKHVTTDMDETFTARALVIATGTQQKHLGIPGEEEYGGRGVSYCAVCDGAFFKGKHLAVIGGGDSAVEEGMYLTQFADQVTVIVRRDQLRAQPMLQDRAKANDKMEFVFNTSPTEIIGDDNKVTGIKLHDNLTNIDRELAVDGVFIYVGNTPMTQPFADLGILDEQGWVKTDEKMATPVEGIFAIGDVRETVLRQIATAVGDGAIAGQQVYHYLEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIU1</accession>
    <name>H4GIU1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5 7">50S ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rplO</name>
        <name evidence="10" type="ORF">PS3_4583</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121974.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4583"/>
    <dbReference type="EnsemblBacteria" id="EHS87171">
        <property type="protein sequence ID" value="EHS87171"/>
        <property type="gene ID" value="PS3_4583"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.521"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="L18e/L15P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_L15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005749">
        <property type="entry name" value="Ribosomal_L15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_L15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_L18e/L15P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934">
        <property type="entry name" value="PTHR12934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="SSF52080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01071">
        <property type="entry name" value="rplO_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L18e/L15P" evidence="9">
        <location>
            <begin position="27"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="144" mass="15472" checksum="49590342ACADC647" modified="2012-04-18" version="1">MKLNELKPSEGSRFKRLRKGRGLSSGHGFTSGRGTKGQKAHGKTRLGFEGGQMPLYRQKPKRGFTSMSHKDYALVSLTTLNQFEDGAEVTPEVLVANGVIKNVKSGVKVLANGKLEKKLTVKANKFSDSAVKAIEAAGGQAEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIW8</accession>
    <name>H4GIW8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nitroreductase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_19226</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nitroreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122001.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19226"/>
    <dbReference type="EnsemblBacteria" id="EHS87140">
        <property type="protein sequence ID" value="EHS87140"/>
        <property type="gene ID" value="PS3_19226"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.557"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02149">
        <property type="entry name" value="NfsB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033878">
        <property type="entry name" value="NfsB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="3">
        <location>
            <begin position="16"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="221" mass="24922" checksum="191BC8FB2E5FEF10" modified="2012-04-18" version="1">MDQVLKPAALKEAAHFRRAIRSYTDQKISDQDLATILEVGRMSPSSKGILDWRIINVKDASLREDLKPHAWGAQKSLPTASDFLIFVARKDVGPDDSLVRETFRQRPGCDNPEKLGELLADHRSFLEHDMQIHDRQGIFEWASKQCYLAFGMMALAASELGIDSCPIEGFNRGQVDQVLLHHNLINPAIEGAVLMMSLGYRDHDPKRPQNRLAMDDFVTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIY2</accession>
    <name>H4GIY2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Shikimate kinase</fullName>
            <shortName evidence="2">SK</shortName>
            <ecNumber evidence="2">2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">aroK</name>
        <name evidence="3" type="ORF">PS3_11864</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + shikimate = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shikimate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122015.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11864"/>
    <dbReference type="EnsemblBacteria" id="EHS87154">
        <property type="protein sequence ID" value="EHS87154"/>
        <property type="gene ID" value="PS3_11864"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.581"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00464">
        <property type="entry name" value="SK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00109">
        <property type="entry name" value="Shikimate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000623">
        <property type="entry name" value="Shikimate_kinase/TSH1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 2" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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    <feature type="binding site" evidence="2">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="172" mass="19107" checksum="1385BFE6F44F3A89" modified="2012-04-18" version="1">MKVVLVGFMGSGKTTVSRLLGQKMNLPVTDLDDVIVEQAGPIPVIFETNGETFFRQIEHDLLLECLNQEGILATGGGTPIREDNVAALTDDDALVVLLKANPETTVARVDNDPNRPLLKEDFSTKHVQEMIDARAEHYQNVCDMQIQTDAYTPDEIAEMIIREVVDREREVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ13</accession>
    <name>H4GJ13_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">VanZ family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_4941</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122046.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4941"/>
    <dbReference type="EnsemblBacteria" id="EHS87125">
        <property type="protein sequence ID" value="EHS87125"/>
        <property type="gene ID" value="PS3_4941"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.630"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006976">
        <property type="entry name" value="VanZ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04892">
        <property type="entry name" value="VanZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="VanZ" evidence="2">
        <location>
            <begin position="41"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="177" mass="20520" checksum="0FFE80E6FE26A027" modified="2012-04-18" version="1">MMVLRWIPLTALLILVTGCFYIAVEERRFTKWQWVSLGGAYLYLLIVIWLCFYPAGLPFIPVGEKPLMYFNHVPYNLHPLQYLTIDWFENILLTIPMGMLTYLLQDRFSLGSMIFVALIPGVTIEAIQMFADLLVNLHRVVDIDDVITNWLGMMIGYGLMKFLAKIGFKRLIAAFEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ20</accession>
    <name>H4GJ20_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_12080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000027">
        <property type="protein sequence ID" value="EHS87096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122053.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000027.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12080"/>
    <dbReference type="EnsemblBacteria" id="EHS87096">
        <property type="protein sequence ID" value="EHS87096"/>
        <property type="gene ID" value="PS3_12080"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.642"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="326"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="360" mass="43277" checksum="EF48811494790A79" modified="2012-04-18" version="1">MKNKASADWGDYLKVFACTAVMLQPILNLVITNQPTGVQFNFGMLYDLVKYTAPAFIWGILYTTMRKHDQSWQAWPYYRQQWHQLGVPTIWWTLIYLLIMPKLQQHEHYHSVKSFLWQAINGNAAPHLWYNTMMLQFIILMPLFWWLHYWLQAHKQRGWIIAIITAIIYGAWIYFYDINVLNGPHQQSWYLLDRIFISFIIYGIGGILAWNFFDKVLPWLQQFWLLMVFAWLITWLWTNQQLFARGFPVNLSRAAYYQPSMTFYSLIVIILVGIIWQSQRSWQIWSSIMHWLANYAYRAYLANVFWQQLLWRYSGIASFRYSHPYITMTALWIGTWLLAFGTAALLHTSWSWTKEKLRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ21</accession>
    <name>H4GJ21_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_3752</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000027">
        <property type="protein sequence ID" value="EHS87097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3752"/>
    <dbReference type="EnsemblBacteria" id="EHS87097">
        <property type="protein sequence ID" value="EHS87097"/>
        <property type="gene ID" value="PS3_3752"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.643"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amoino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="430"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="AA_permease" evidence="7">
        <location>
            <begin position="20"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="464" mass="51282" checksum="CDF80C3713F20C6C" modified="2012-04-18" version="1">MEGSNVNPDGTRRELSNLNVQMIAIGGAIGTGLFLGSGSTIHRTGPSILLVYIIIGFFFYLMMRAIGEMMYTDPSKHTFVAFIHRYMGSKYGRFAEWNYWLTIVLTAMAEMIAISTYVRTWFPSWPGWLVQIVFLLAMMGLNLFVVSAFGKAETVMALIKIFAILALIVIGIIMVIMHYKSPTGHIASFTNVLGNFKMFPNGIDQFIFAFPMVFFAFQGIEFVGITTAETKNPRQVLPKAINQTLLRILIFYVGALFVIMSITPWRQINADSSPFVQIFSLAGIPAAGDIINLVVIIAATSSLNSFLFSGGRHLYQLALEAGPNKMQVFKTISLNGIPVYAVIGTAVLTLFAPIITATGMTDAFSFVTSISSDIYIIVCVLTMISHIRYRQSSEFLDHEFQMPLAKISSPLTILFFVVIYASLFFSPTSIWAAVGASVWAIGFFLVIAYRDKHGAYPKDGSLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ26</accession>
    <name>H4GJ26_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">D-alanine--D-alanine ligase</fullName>
            <ecNumber evidence="9">6.3.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">D-Ala-D-Ala ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">D-alanylalanine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ddl</name>
        <name evidence="13" type="ORF">PS3_11032</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + 2 D-alanine = ADP + D-alanyl-D-alanine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11224"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57416"/>
            <dbReference type="ChEBI" id="CHEBI:57822"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10">Binds 2 magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the D-alanine--D-alanine ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.4" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122059.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11032"/>
    <dbReference type="EnsemblBacteria" id="EHS87051">
        <property type="protein sequence ID" value="EHS87051"/>
        <property type="gene ID" value="PS3_11032"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.659"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008716">
        <property type="term" value="F:D-alanine-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00047">
        <property type="entry name" value="Dala_Dala_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000291">
        <property type="entry name" value="D-Ala_lig_Van_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005905">
        <property type="entry name" value="D_ala_D_ala"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011095">
        <property type="entry name" value="Dala_Dala_lig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011127">
        <property type="entry name" value="Dala_Dala_lig_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07478">
        <property type="entry name" value="Dala_Dala_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01820">
        <property type="entry name" value="Dala_Dala_lig_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039102">
        <property type="entry name" value="Ddl/VanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01205">
        <property type="entry name" value="D_ala_D_alaTIGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00843">
        <property type="entry name" value="DALA_DALA_LIGASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00844">
        <property type="entry name" value="DALA_DALA_LIGASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0133">Cell shape</keyword>
    <keyword evidence="8 9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 9 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="12">
        <location>
            <begin position="141"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR039102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS87051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="377" mass="42315" checksum="A7A245A48CFA550B" modified="2012-04-18" version="1">MTDKKLHIALLFGGNSSEHDVSKRSAHNIFDGMDRDKYDVSLFMFTKDGILLDHQSSQRIFDGEPEDQVVKEAYAKMDLNSPLAPIAALNEVPEIDFFYPVIHGNLGEDGTVQGLFRLLKKPYVGSGIAASAMAFDKELTKRILNQAGIRNTKYILVTPDNQASYGWDQVKAELGDLVFVKPAKQGSSVGIHKVRNAEEYQAALTDALTYDYKVLIEEAVPNPREIEISILGNDQPIASKLGGLRIPEGDDFYDYNNKFVDASGVVFDLPVIVAEPLQKEITQMALDAYQALEMKGMCRMDFLVDENDVPYLSEPNTLPGFTNISLYPQMWNVSGISYSELIDRLIELGIEEFNHQNQLRYDFQSLGEEHVGRKIYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ31</accession>
    <name>H4GJ31_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP synthase gamma chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">ATP synthase F1 sector gamma subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">F-ATPase gamma subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">atpG</name>
        <name evidence="11" type="ORF">PS3_10561</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATPase gamma chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122064.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10561"/>
    <dbReference type="EnsemblBacteria" id="EHS87056">
        <property type="protein sequence ID" value="EHS87056"/>
        <property type="gene ID" value="PS3_10561"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.668"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12151">
        <property type="entry name" value="F1-ATPase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00815">
        <property type="entry name" value="ATP_synth_gamma_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035968">
        <property type="entry name" value="ATP_synth_F1_ATPase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000131">
        <property type="entry name" value="ATP_synth_F1_gsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693">
        <property type="entry name" value="PTHR11693"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00231">
        <property type="entry name" value="ATP-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00126">
        <property type="entry name" value="ATPASEGAMMA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52943">
        <property type="entry name" value="SSF52943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01146">
        <property type="entry name" value="ATPsyn_F1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0139">CF(1)</keyword>
    <keyword evidence="5 10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="307" mass="33789" checksum="D8374946D99D0B60" modified="2012-04-18" version="1">MPASLAAVKRRIESTKSTRQITAAMQMVSTAKLNQIQHHTTTYQVYADKVKSILASLVKSHAIDSLGGNKGMSSLFHPREVKKTGILVITSDRGLVGSYNSNVIKATLDLMREKNLTAANTEFLTVGRTGLDFFQKRGMDVVYEYTGVSDVPKYAEVRHIVNAAVQKYQDGAYDELYISYSHYVNRISTEVKSEKFLPISADDLVDPNEKTSTSTDENSVPAAEYEIEPSLEEFVEMVVPQYAESLIYGAILDAKTSEHSSSSNAMSSATDNADDIISTLQLQYNRARQAAITTEITEITGGMTAQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ32</accession>
    <name>H4GJ32_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP synthase subunit alpha</fullName>
            <ecNumber evidence="10">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">ATP synthase F1 sector subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">F-ATPase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">atpA</name>
        <name evidence="14" type="ORF">PS3_7777</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="10"/>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122065.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7777"/>
    <dbReference type="EnsemblBacteria" id="EHS87057">
        <property type="protein sequence ID" value="EHS87057"/>
        <property type="gene ID" value="PS3_7777"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.669"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18113">
        <property type="entry name" value="ATP-synt_F1_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01132">
        <property type="entry name" value="F1_ATPase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01346">
        <property type="entry name" value="ATP_synth_alpha_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000793">
        <property type="entry name" value="ATP_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038376">
        <property type="entry name" value="ATP_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033732">
        <property type="entry name" value="ATP_synth_F1_a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005294">
        <property type="entry name" value="ATP_synth_F1_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00306">
        <property type="entry name" value="ATP-synt_ab_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039088">
        <property type="entry name" value="F_ATPase_subunit_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="SSF50615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00962">
        <property type="entry name" value="atpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0139">CF(1)</keyword>
    <keyword evidence="10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATP-synt_ab_N" evidence="13">
        <location>
            <begin position="25"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_ab" evidence="11">
        <location>
            <begin position="149"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_ab_C" evidence="12">
        <location>
            <begin position="371"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="169"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Required for activity" evidence="10">
        <location>
            <position position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS87057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="510" mass="55044" checksum="20A6C6A0E0BFD4B1" modified="2012-04-18" version="1">MSIKTEEISSLIKKQLANYEATISVEETGTVTYVGDGVARADGLENAMAGELLEFSNGVYGMAQNLESNDVGIVILGEYTGIREGDTVKRTGRIMEVPVGDALLGRVVDPLGRPIDGLGDIKTEKTRPIEHKAPGVMERKSVSVPLQTGIKVIDALVPIGRGQRELIIGDRKTGKTSIAIDTIINQKDQDMICIYVAIGQKESTVRAQVETLRQFGALDYTIVVSASASNPAPMLWIAPYAGTAMGEEFMFGGRDVLIIYDDLSKQADAYRELSLILRRPPGREAYPGDIFYTHSRLLERAARLSDDLGGGSMTALPVIQTQAGDVSAYIPTNVISITDGQIFLNADSFYAGQRPAIDAGTSVSRVGGDAQIKAMKKVSGTLRLDIASYNELASFAQFGSDLDAATQAKLARGQRTVELLKQGLHAPQPVEQQVVTLYALAHGYVDDVPVDDIARFESELASYMRGNHQDLYDQITQSGQLPGDDSLDDAIKAFKQSFQTSAEASQAQND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ43</accession>
    <name>H4GJ43_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptide chain release factor N(5)-glutamine methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.297</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_6962</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-methionine = H(+) + N(5)-methyl-L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42896"/>
            <dbReference type="Rhea" id="RHEA-COMP:10271"/>
            <dbReference type="Rhea" id="RHEA-COMP:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
            <dbReference type="EC" id="2.1.1.297"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.297" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122076.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6962"/>
    <dbReference type="EnsemblBacteria" id="EHS87068">
        <property type="protein sequence ID" value="EHS87068"/>
        <property type="gene ID" value="PS3_6962"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.682"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102559">
        <property type="term" value="F:protein-(glutamine-N5) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004556">
        <property type="entry name" value="HemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040758">
        <property type="entry name" value="PrmC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019874">
        <property type="entry name" value="Release_fac_Glu-N5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17827">
        <property type="entry name" value="PrmC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00536">
        <property type="entry name" value="hemK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03534">
        <property type="entry name" value="RF_mod_PrmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PrmC_N" evidence="8">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="MTS" evidence="7">
        <location>
            <begin position="115"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="286" mass="32511" checksum="2061FA425B04C9EA" modified="2012-04-18" version="1">MSKWTYFEAQQWAINQLADTGMDLQSPAFILSMIHDWTYTQLISHNRETMPDDEVVRYQKAVQQIANHVPPQYVVGKAPFFGRTFIVNQDVLIPESETEELIEWVLNYYADDQPLNVLDLGTGSGVIGITLALERPNWQVTLSDISPAALRVAKANQRLHGTNLRQIESDLFAQIDDQYDLIVTNPPYIDHQEVGLMDQAVKEYVPPLALYANEHGLGIYRRIFEEAGKCLKVGGQIFGETGFNQEVTIQALLNRIAPQANIETRHDINGNMRMIRVWDLLESQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ49</accession>
    <name>H4GJ49_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative esterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13589</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122082.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13589"/>
    <dbReference type="EnsemblBacteria" id="EHS87074">
        <property type="protein sequence ID" value="EHS87074"/>
        <property type="gene ID" value="PS3_13589"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.689"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase" evidence="1">
        <location>
            <begin position="22"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="336" mass="37315" checksum="6E3EF82463EB9C95" modified="2012-04-18" version="1">MYQKTQASLKAMVDQGIVPALAMAIIDPRGDRQVFFGTTDGQANHPVNANHIFDLASLTKVLGTLPVILNLLGQHALSLDDPVNKYLPIVQESRLTIRHLLTHTSVMDGYIAHRDQLRAPELKTALLSTQMSVDNLGRLIRYSDTNFLYLGWIVENILGQPIQTVIEQRLLQPLEITGLTFHPDPQLAVPTADPAGNRFQGIVHDPKARILKDQCGSAGLFGTLDGVQQLLSLWLANNLKGIIEPSLTSMMQTDQTAMPGHHLRGLGWKLMHATAPDHHFVVTHTGFTGTWVMVDFKQNQALIVLSNRVYPNGQNDQFLDLRDQVMATYLGEKEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ50</accession>
    <name>H4GJ50_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Multidrug ABC transporter ATP-binding and permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_15064</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15064"/>
    <dbReference type="EnsemblBacteria" id="EHS87075">
        <property type="protein sequence ID" value="EHS87075"/>
        <property type="gene ID" value="PS3_15064"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.690"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="36"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="370"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="402"/>
            <end position="409"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="610" mass="68769" checksum="6B072117E9D68167" modified="2012-04-18" version="1">MRNGNRRGKPVEKARHFWPTTKRLISYLRPWKWGVLLSILMAIVSVVLNIVSPKILGQATTDIYDGILKGVQQMKLGLHVTKYPIDFNHVGQICLIVVALYILSGLFSFGQQVLMTWISQKVVYNLRQDFKEKMGRLPIKYYDQHSNGDLMSRMVNDMDNISGTLQQGLIQIVTSIITFVGVLGMMLSISWKLTLVACITLPLSAIIIALVAPTAQRLFAHQQATLGKLNDQVEEIYAGHTIVRTFNKEADEEDKFAENNQRYYQAAWKAQFFSILMYPLMAFVRNLGYLMVAIVGTIEVAHGQITLGNVQAFLQYTNQFSQPITQIANLSSTIQQTIASAERIFTVLDEAEMDNQLLDTTVLPAPQPKIEFKDVAFSYGDEPLIEDFNLSAGKDHMVAIVGPTGAGKTTIINLLERFYDVDAGHIYLDGQDTRSMTRQEVRQRISMVLQDTWLFTGTIYDNIKYGREDATEEEVYEAARQAHADNFIRQLPEGYQTVLDETALNISQGQRQLLTIARAFLANPEVLILDEATSSVDTRTETLIQNAMNDIQQHRTSFVVAHRLSTIRNADRIIVMNHGHIIETGNHDSLMAQNGFYADLYNSQFLGADI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ62</accession>
    <name>H4GJ62_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ThrE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_16002</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122095.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16002"/>
    <dbReference type="EnsemblBacteria" id="EHS87087">
        <property type="protein sequence ID" value="EHS87087"/>
        <property type="gene ID" value="PS3_16002"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.709"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010619">
        <property type="entry name" value="ThrE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06738">
        <property type="entry name" value="ThrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="ThrE" evidence="2">
        <location>
            <begin position="12"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="249" mass="27096" checksum="9FCA4DAABEE5BEFD" modified="2012-04-18" version="1">MEDQERNLAITTCILAGQILIENGSNMDRVNDTMYRMGSAAGLKSFQVFTTVTGIVASSDHQPNAQAVDIRVRKNNLRKIAQVNSVSRQFSAGELSLQQTYQRLSEVDRDQTNLPEWLQCLAAAILSAVLMVIFTEDVRDIAYGFVIGGLGYWTFLRLFGRFQIKYISEFCSALVIGICTVLLGKLGLVHDTSATIVGSIMPLVPGVPLTNAARDFVSGNLISGPSRALEALLTAASIGCAIVIVLRYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJA1</accession>
    <name>H4GJA1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phage head morphogenesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_11373</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000031">
        <property type="protein sequence ID" value="EHS87013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122134.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000031.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11373"/>
    <dbReference type="EnsemblBacteria" id="EHS87013">
        <property type="protein sequence ID" value="EHS87013"/>
        <property type="gene ID" value="PS3_11373"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.785"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006528">
        <property type="entry name" value="Phage_head_morphogenesis_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04233">
        <property type="entry name" value="Phage_Mu_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01641">
        <property type="entry name" value="phageSPP1_gp7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phage_Mu_F" evidence="1">
        <location>
            <begin position="204"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="328" mass="37489" checksum="BCB551E9277906F3" modified="2012-04-18" version="1">MVKKINQQTSSYWEQRRAKEEEWIKENLSNDEAFNRQLQVYYDQAINNIDEMIKANTDLITDKATGKVTEADVRSYETKAKSIVLEAQKRLKAGQKVQYGDYDKQINNHLKIYNATMRINRLEWIKSNIGLEMVQLGLNVDADLRDKLSADYQKEIKRQSGIMGESVGKLSSSSITAQIILAQTKGATFSQRLWVDQDVLKSKLDQVIANGIIQGQSNQRVARNLRDQLKSTVDNQRYVTERLARTESSRVQAQAEILSMKGNGFKYCIWIDEPRACAECKQIANKDNGWGKGVYLVDDVPFLPAHPNCRCVLSAYWVERLSLENLAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJA6</accession>
    <name>H4GJA6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Prophage Lp2 protein 17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_16984</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000031">
        <property type="protein sequence ID" value="EHS87018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122139.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000031.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87018">
        <property type="protein sequence ID" value="EHS87018"/>
        <property type="gene ID" value="PS3_16984"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.796"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006505">
        <property type="entry name" value="Phage_nucleotide-bp"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01618">
        <property type="entry name" value="phage_P_loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="214" mass="24478" checksum="6E009E977A25A2D1" modified="2012-04-18" version="1">MRFYESGKIPDRQHMYFVYGDGGTGKTSLFKQFPGKKLLISFDMSTNPLQGDESIDVAILDESEKPRIQSVLDNFLTRAISSGRYQSICLDNVTALQNYVLENIDGQSKDGRQNYQKLQLWFRDLGSYLRNVNVTVYVTAHSIDKGADVINGRYEADMNVKTFNALTSMFDVVGRIYMQDGQRMIDLDPEQGNHAKNRIDDRKLIKAEELLQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJC0</accession>
    <name>H4GJC0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5-oxoprolinase subunit A</fullName>
            <shortName evidence="1">5-OPase subunit A</shortName>
            <ecNumber evidence="1">3.5.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-oxoprolinase (ATP-hydrolyzing) subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pxpA</name>
        <name evidence="2" type="ORF">PS3_2168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the cleavage of 5-oxoproline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-oxo-L-proline + ATP + 2 H2O = ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10348"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58402"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.5.2.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a complex composed of PxpA, PxpB and PxpC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LamB/PxpA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.9" evidence="1"/>
    <dbReference type="EMBL" id="AICN01000033">
        <property type="protein sequence ID" value="EHS86989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122153.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000033.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2168"/>
    <dbReference type="EnsemblBacteria" id="EHS86989">
        <property type="protein sequence ID" value="EHS86989"/>
        <property type="gene ID" value="PS3_2168"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.821"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00691">
        <property type="entry name" value="PxpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005501">
        <property type="entry name" value="LamB/YcsF/PxpA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30292">
        <property type="entry name" value="PTHR30292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03746">
        <property type="entry name" value="LamB_YcsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="248" mass="26312" checksum="53D25CDF9B5E811B" modified="2012-04-18" version="1">MITVDFNSDLGESFGNYTIGDDAMVLPMITSANVACGFHAGDPDVMAKTVTLAQQNQVAVGAHPGYPDLAGFGRRKMAMSHQEVADMVTYQVAALQGFLPDHHLHHVKPHGALYNAAAKDFELALAICEGIKRVDPSVPLYGLANSELIRAGQRSGLPVRSEVFADRAYQADGSLVPRSQMGAVLTDPQQVAQRALTMVTTGTVTAITGETVKIVADTICVHGDNAAARQLVTTMRAVFDDAGINIHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJE1</accession>
    <name>H4GJE1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Drug/metabolite exporter family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_14583</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EamA transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000035">
        <property type="protein sequence ID" value="EHS86890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122174.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000035.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14583"/>
    <dbReference type="EnsemblBacteria" id="EHS86890">
        <property type="protein sequence ID" value="EHS86890"/>
        <property type="gene ID" value="PS3_14583"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.851"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="6"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="3">
        <location>
            <begin position="160"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="300" mass="32830" checksum="C98807D8C39557F7" modified="2012-04-18" version="1">MSKQTKGIILAIAGASLWGGSGAAVQYLFTDTNLKTEWIVAIRMLFAGLMLLIWSVGKQRSQLVMILHDHVSWWKIILFALLGVTNSQLSYFFAVKYSNAPTATVLQYLSPILIIIWMAIVTKRWPRRIDLISIGLALVGTFYLVTGGNIHSLMLTHQALAWGLWSAVAAALYTLLPRQLMQKYDASVMCGVAMFIGGIILSPILFIEAGPQLNILDWLLVAYVVIFGTALAYTLFVQSIRYVSPSVTGILSAFEPLVATILAVTLLGTRLTFATTLGSLLIVATTLIQSIPTNPLAKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJE8</accession>
    <name>H4GJE8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_13034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000036">
        <property type="protein sequence ID" value="EHS86871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122181.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000036.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13034"/>
    <dbReference type="EnsemblBacteria" id="EHS86871">
        <property type="protein sequence ID" value="EHS86871"/>
        <property type="gene ID" value="PS3_13034"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.871"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008841">
        <property type="entry name" value="Siphovirus-type_tail"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05709">
        <property type="entry name" value="Sipho_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="278" mass="32224" checksum="122763C01D36CBD7" modified="2012-04-18" version="1">MQQPNIYLKIGDEEEFNPADCIKGLKYLGVANESSSPQWTNLYQDYQGRDGSQFLGQTLAKRTVNEPFFLHFGQWQDFQLAKHEIYRLFADREKVRVRTDVNPDKVYFGYVTPFDIAPLEDYSHEAKFTIPFDVPDGYRYSRYRSDAQPGEGQYGMDIANDLMNYHFTSMSFRVYNASDITIDPYYQSHDLRLLVTFEGNSCKVTNSTTNTEWRYLKSCEGKQLLFDGKTLTTYENGKNVNVNTNYGYISLKPGWNEITVSEASSIDITFSFPFIYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJF9</accession>
    <name>H4GJF9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Site-specific tyrosine recombinase XerS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_8976</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000037">
        <property type="protein sequence ID" value="EHS86720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122192.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000037.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8976"/>
    <dbReference type="EnsemblBacteria" id="EHS86720">
        <property type="protein sequence ID" value="EHS86720"/>
        <property type="gene ID" value="PS3_8976"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.909"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13495">
        <property type="entry name" value="Phage_int_SAM_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="5">
        <location>
            <begin position="12"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="4">
        <location>
            <begin position="162"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS86720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="354" mass="40857" checksum="7B391908FA0D7347" modified="2012-04-18" version="1">MESDKYFHLIKIELEKLPDYVNEYYLGTNHSVTTTYQYLTEIRRFMDWLRASGVSKAPTNQEIPTSDLEHLSRSDVMLYIDYLQHTTNAQGRVNSPTSVNRSINSLRSLFKFLTVTADNNHGEPYFDRNVMLKIDSLNNTKTLNYRAHALASHMYRGQMKFDFINFIQNEYAHKCDKRALPGFEMNRERDIAIIALILGTGVRVSEAANLNLADLNWQQAMLDVTRKGGQRDSVPIAPWSMEYIKSYAAIRAERYQAHKKDQAFFLTLYHGETRRMTTNSIEKMVKKYSTAFGHPLTPHKLRHTLASELYGITKDQVLVSQQLGQKGTSATDLYTHVDQQQQRAALDEISQERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJG9</accession>
    <name>H4GJG9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative 3-methyladenine DNA glycosylase</fullName>
            <ecNumber evidence="5">3.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_15353</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the DNA glycosylase MPG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000039">
        <property type="protein sequence ID" value="EHS86612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122202.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000039.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15353"/>
    <dbReference type="EnsemblBacteria" id="EHS86612">
        <property type="protein sequence ID" value="EHS86612"/>
        <property type="gene ID" value="PS3_15353"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.920"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003905">
        <property type="term" value="F:alkylbase DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00540">
        <property type="entry name" value="AAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00527">
        <property type="entry name" value="3MGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003180">
        <property type="entry name" value="MPG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036995">
        <property type="entry name" value="MPG_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10429">
        <property type="entry name" value="PTHR10429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02245">
        <property type="entry name" value="Pur_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="SSF50486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00567">
        <property type="entry name" value="3mg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS86612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="212" mass="24167" checksum="922F88CF03068DC2" modified="2012-04-18" version="1">MDAYQAYFSSQPTTAIAQDLLGRLLIYDSDQGHFAGYIVETEAYLGVQDSASHAFNHRRTNYSESLYGDPGDLYIYQIRANYCFDVVVQVRDEPQGILIRAIEPLANLDQMVKNRHQTGVNLTNGPGKVMQALGIQDRHLDGLPMDQAPLKIDLSQRRQPQKIMMTPRIGVNQDGDSGQDLCRFYVWGNPYVSKSKRRDDDYDHHGWLKKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJJ5</accession>
    <name>H4GJJ5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Two component response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_14063</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122228.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14063"/>
    <dbReference type="EnsemblBacteria" id="EHS86505">
        <property type="protein sequence ID" value="EHS86505"/>
        <property type="gene ID" value="PS3_14063"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.964"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="7">
        <location>
            <begin position="124"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="124"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="223" mass="25156" checksum="C29678E861E01DD0" modified="2012-04-18" version="1">MKVLVAEDQADLSRLLKIAIEHEGFTVDIANNGQEAVDLAAQNAYDVMILDIMMPVKDGITALKEIRQAGDDTYIIMLTAMTEIDDRVNGLDAGADDYMTKPYSIKELMARLRTLDRREKQYEQPLLQFGDLTLDPDEQRIESHNSISLSGKETKLLAIFMSNPNKEIPSQQLLNTVWDDDDQAEPADLWIYISYLRQKLQSINAHVQLVGNQDGPFALTTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJN1</accession>
    <name>H4GJN1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CRISPR-associated Csn2 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_4791</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000044">
        <property type="protein sequence ID" value="EHS86254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122264.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000044.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4791"/>
    <dbReference type="EnsemblBacteria" id="EHS86254">
        <property type="protein sequence ID" value="EHS86254"/>
        <property type="gene ID" value="PS3_4791"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1020"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010146">
        <property type="entry name" value="CRISPR-assoc_prot_Csn2-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038600">
        <property type="entry name" value="Csn2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09711">
        <property type="entry name" value="Cas_Csn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01866">
        <property type="entry name" value="cas_Csn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="224" mass="26103" checksum="DDDE48D5F02A1460" modified="2012-04-18" version="1">MKVVYETHQTIDVKPGKITVLATNNQTVYLELIRGMKDMNESIHFYDDDYKEVDHNKAMDWDGDVVADLSLEKTYSTEIIKYINQSLTDEERRQLDQAGLEFYTAIQDKLLMVDIPLEVSFDGDIKRMMKHAQIHFPKEINNDPYGIIEADLKIHLECDDQSIIGLTNVAHYLTLDQISHLVEINDHLQTKVLLVEFTESRNQDAYPNCDYYYIDEDFVDWGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJQ1</accession>
    <name>H4GJQ1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dipeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_12672</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000046">
        <property type="protein sequence ID" value="EHS86233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122284.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000046.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12672"/>
    <dbReference type="EnsemblBacteria" id="EHS86233">
        <property type="protein sequence ID" value="EHS86233"/>
        <property type="gene ID" value="PS3_12672"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1043"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016805">
        <property type="term" value="F:dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010964">
        <property type="entry name" value="M20A_pepV-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01887">
        <property type="entry name" value="dipeptidaselike"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="465" mass="51217" checksum="E57F07BE8C7D404A" modified="2012-04-18" version="1">MNWQELANAQRDHYLRDLISLIKIPSVRDEEKASPEYPLGPALAQALKAFLEMADQDGFRVKNIDNVVGYAEWGDGEETLAILAHLDVMPAGNGWDTDPFDPVIKDGNIYGRGASDDKGPGLAAYYALKELKDQGIEFNKRVRFIVGTDEESNWTGMKRYFEVEPAPTLGFSPDAEFPVINGEKGQVSLKLSGSGDNDGQVQLIKFDAGLRFNMVPREATAVLSGTNVDQLQEAFDQFIQAEPVSGSMTADGDQVKFEILGKAAHGAEPEKGINAGTYLAKFLKQVPLNGQAANFVTFLADYLHLDTRVRNFKADFRDEVMGEMTMNAGILQFDQTKGIQIDMNFRYPKGITPTEINQQIGQVANQLGLTVTTGRNQDPHYVDPTDPIVQTLMKAYRDQTGDQSAQPLVVGGGTYGRLMKRGVAFGALMPTTPNVMHQANEFQPVEDLLLSMAIYMQAIYDLVTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJQ9</accession>
    <name>H4GJQ9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">APC family amino acid-polyamine-organocation transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_12434</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000047">
        <property type="protein sequence ID" value="EHS86209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122292.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000047.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS86209">
        <property type="protein sequence ID" value="EHS86209"/>
        <property type="gene ID" value="PS3_12434"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1062"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="364"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="387"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="417"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="444"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS86209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="472" mass="51194" checksum="712BD974AD037A7D" modified="2012-04-18" version="1">MGSSIFRKKDVNSLLHQRSPLKRTLRTMDLTFLGIGAIIGTGIFVLTGKGALTAGPALSVSFLIAGICCGFAALCYAEFASLAPISGSAYTYSYIAFGEIIAFIIGWDLILEYSLATATVSAGWSGYLVSLLDDFGIHLPTILTAAAGTTPGVTTYFNLPAFLIVLIITWIISIGITQTKEVNNVMVVIKIGVVLLFIICTVWFVKAANWHPFNPYGWYSYHGGTATGIIPAASIVFFSFIGFDAVSSSAEETINPSKTLPRGIILSLVISLILYVIMTLIMTGIVKYTGFANYLNAPIMAVLHKTGQLWLSIIVSLGAIVGMTTVMLVCLYGQSRISYSMSRDGLFPKFFSDIHPKYQTPAKGTWFFGIVTALAGGLINLNILSELVNIGTLTAFILVSAGILYMRRKQPDLKRGFRAPGVPFTPLLSIGFCLVLIAGLNWETWVRFLVWLAIGLTLYFAYGRKHSKINAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJR9</accession>
    <name>H4GJR9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Amino acid ABC transporter permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_20421</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000047">
        <property type="protein sequence ID" value="EHS86219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20421"/>
    <dbReference type="EnsemblBacteria" id="EHS86219">
        <property type="protein sequence ID" value="EHS86219"/>
        <property type="gene ID" value="PS3_20421"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1077"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043429">
        <property type="entry name" value="ArtM/GltK/GlnP/TcyL/YhdX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614">
        <property type="entry name" value="PTHR30614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="24"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="222" mass="24735" checksum="826A520FF509BEFB" modified="2012-04-18" version="1">MGSLTGLIDLHLAISSIPTILSGLPMSLFLLVTCFILANILGFLLVLIRQSRWAILRWIARFYISFFRGVPMLVILFITYFGFNMDAIPAAVLSFTIAPAAFISEYYRSALLGVDLGQYEAARSLGMNRYVVLRYVILPQALRIALPSLGNVMVDMFKGTSLAAMITVSEMFMDAKIVAGANQDYMSIYITLAVIYWLLCCLLSWGQGWLEGRLDFVAKAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJW1</accession>
    <name>H4GJW1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_20628</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000050">
        <property type="protein sequence ID" value="EHS86035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122344.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000050.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20628"/>
    <dbReference type="EnsemblBacteria" id="EHS86035">
        <property type="protein sequence ID" value="EHS86035"/>
        <property type="gene ID" value="PS3_20628"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1144"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="PTHR43827"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="4">
        <location>
            <begin position="29"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="3">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="287" mass="32072" checksum="E1269E65F4B3638E" modified="2012-04-18" version="1">MTVPTFTLRDGNQIPSFGFGVFQIPADGSTKVAVAKALELGYRHIDTATAYFNEAEVGAAIQESGIPREEIWVTTKLWLQDYAYEDAKKGIDTSLTKLGLDYVDLMLLHQPYGEVEQAWQALEEAQAAGKIKSIGVSNMTPKIYQKFVPNFKTKPVVNQVEFNPYFQQEELRQTMLEDGVVIEAWAPLGQGNADLFQEPVLQEIAERHHKNVGQVILRFEHQLGIIVFPKSTHNERIKSNIDIFDFALSNVEMDAIKALDKGKGSHDPDAPGVGEWLLNAFDVHAND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJW8</accession>
    <name>H4GJW8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cupin superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_4339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS85985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122351.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4339"/>
    <dbReference type="EnsemblBacteria" id="EHS85985">
        <property type="protein sequence ID" value="EHS85985"/>
        <property type="gene ID" value="PS3_4339"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1153"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="49"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="138" mass="15286" checksum="80447EFE799774EE" modified="2012-04-18" version="1">MAKNEEAVKNDMFGFGEENTAYAQYFIGKSYLNGLVPAKDDVTVGVANVNFEPGCRNNWHIHHDGYQIIMCTAGEGWYQIEGQPAQKMHPGDVAVFHDGVKHWHGATKDSWFSHVAIGAGSTEWLEPVDDEAYGKLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJX5</accession>
    <name>H4GJX5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Na-H antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_20880</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS85992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20880"/>
    <dbReference type="EnsemblBacteria" id="EHS85992">
        <property type="protein sequence ID" value="EHS85992"/>
        <property type="gene ID" value="PS3_20880"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1166"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="PTHR10110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0915">Sodium</keyword>
    <keyword evidence="5" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="1 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Na_H_Exchanger" evidence="7">
        <location>
            <begin position="5"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="454" mass="51783" checksum="7F85F4CABF449EEB" modified="2012-04-18" version="1">MVSTKFKSIVSMTVVMVLVILLVAGFTIQSLFKIGLPLAFLLAAISIPTDATATDAVTDGLIMPKRPNILLKLESLFNDASGLILLSMATLWFQNGYINYQQTLMDFLISAFGGATLGYVSSWLLMMLRQQMFTSKVNFLNNTYNNGTPMKVIFFATPFLIYFIAEEIHVSGIIAVVVAGLVGNTEAQRSKLLNPMVYYDSVRLIEMISEILNGVVFVVLGIVLMRTYRDEQITYGSWTWIYIGIVLYLSNLIVRFLYSKFVMREKAHTAKLFALGGVHGAVTFALVMMLEHATLSTRQFNLAIMSESVLIILSLLVPSILFRFVLDKDVPDDEMIEELTSMRVAMVEHARQQLNEIYVPDRMKPILEYDLNAQIGQTSTRDFIKQWQKASRVVGLDQNQTIFLNEIYHYIFDQERDYLAMIEHRESKYHEVFNILYKEIMMAEMVSLRDDNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJZ2</accession>
    <name>H4GJZ2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">1-deoxy-D-xylulose-5-phosphate synthase</fullName>
            <ecNumber evidence="5">2.2.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_7950</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transketolase family. DXPS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.7" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS86009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7950"/>
    <dbReference type="EnsemblBacteria" id="EHS86009">
        <property type="protein sequence ID" value="EHS86009"/>
        <property type="gene ID" value="PS3_7950"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1202"/>
    <dbReference type="UniPathway" id="UPA00064">
        <property type="reaction ID" value="UER00091"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008661">
        <property type="term" value="F:1-deoxy-D-xylulose-5-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052865">
        <property type="term" value="P:1-deoxy-D-xylulose 5-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02007">
        <property type="entry name" value="TPP_DXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005477">
        <property type="entry name" value="Dxylulose-5-P_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43322">
        <property type="entry name" value="PTHR43322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13292">
        <property type="entry name" value="DXP_synthase_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0784">Thiamine biosynthesis</keyword>
    <feature type="domain" description="Transket_pyr" evidence="8">
        <location>
            <begin position="230"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS86009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="536" mass="58757" checksum="A6BE6B08BBE63F6A" modified="2012-04-18" version="1">MTIALHYVFNSPVDKLIFDVSHQSYVHKMLTGRAQAFLDPAHYDDVSGYTNPKESEHDLFTIGHTSTSLALASGVSKARDVKGDQYNVVAVIGDGSLSGGMAYEGLNQIATENTNLIVIVNDNDQSIAKNPAGGIYTALRTLRQSKGTAEDNIFKALGYDYHYLDEGNDLEQLIKLFQDVKDTDHPVLLHIHTQKGKGIPFMEENREAFHAGGPYDPKTGAYQSFGNNEPTYSSVTTDYLMDKAQKDADVVTISSGTPMFAFDQQQREALGSQFVDVGIAEEEAATMSAGLAKNGAKPVWFVASTFMQRTYDQWSHDIALNNLPATVLVYMASVSGMNDESHLGFFDIPFLAHIPNVVYLAPTSKEEHLAMMDWAIDQKDHPVAIRVPVGPLRETGAVDHTDYSILNKNQVTQKGSQVALFGLGNFYSPAEEVAKKLADDHGIQATIVNPKFITGLDTELLDSLADDHQVVVTLEDGILEGGYGQMVASYLGNTDLKVQNYGVKKAYPDRYNPQDYLAQNGITVDNIVNQVVQSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJZ5</accession>
    <name>H4GJZ5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 13 15">tRNA (guanine-N(1)-)-methyltransferase</fullName>
            <ecNumber evidence="6 13 15">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">trmD</name>
        <name evidence="17" type="ORF">PS3_14620</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 13 15">Specifically methylates guanosine-37 in various tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13 15">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 13 15">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 13 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13 15">Belongs to the RNA methyltransferase TrmD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="6 13 15"/>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS86012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122378.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14620"/>
    <dbReference type="EnsemblBacteria" id="EHS86012">
        <property type="protein sequence ID" value="EHS86012"/>
        <property type="gene ID" value="PS3_14620"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1208"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N(1))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18080">
        <property type="entry name" value="TrmD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1270.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00605">
        <property type="entry name" value="TrmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002649">
        <property type="entry name" value="tRNA_m1G_MeTrfase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023148">
        <property type="entry name" value="tRNA_m1G_MeTrfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016009">
        <property type="entry name" value="tRNA_MeTrfase_TRMD/TRM10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46417">
        <property type="entry name" value="PTHR46417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01746">
        <property type="entry name" value="tRNA_m1G_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000386">
        <property type="entry name" value="tRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00088">
        <property type="entry name" value="trmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="10 13 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 13 15" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA_m1G_MT" evidence="16">
        <location>
            <begin position="23"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="13 14">
        <location>
            <begin position="135"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000386-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EHS86012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="250" mass="28230" checksum="855F1811B86EF716" modified="2012-04-18" version="1">MRIDILSLFPNMFAAPLGESIVGRAQEDGLVDLHVTDFRQFTTDKHRHVDDAPFGGGAGMLLQAQPIFDAMDHIKKEIDGQLPRGRVILMDPAGRKFDQDYAQELAEEEHLTFICGHYEGYDERIRQLVTDEASLGDYVLTGGELAAMVMIDATVRFVPGVLGNQESPTGDSFSNGLLEYPQYTRPADFRGMQVPEVLTSGNHQKIAEWRMRESLRRTLLRRPDLLEKAHLSPEQAIMLQDLKLEYEDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJZ7</accession>
    <name>H4GJZ7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">30S ribosomal protein S16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpsP</name>
        <name evidence="4" type="ORF">PS3_8994</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the bacterial ribosomal protein bS16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS86014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122380.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8994"/>
    <dbReference type="EnsemblBacteria" id="EHS86014">
        <property type="protein sequence ID" value="EHS86014"/>
        <property type="gene ID" value="PS3_8994"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1210"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00385">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000307">
        <property type="entry name" value="Ribosomal_S16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023803">
        <property type="entry name" value="Ribosomal_S16_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12919">
        <property type="entry name" value="PTHR12919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00886">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54565">
        <property type="entry name" value="SSF54565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00002">
        <property type="entry name" value="S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="91" mass="10519" checksum="9C6B4407FD362864" modified="2012-04-18" version="1">MSVKIRLKRMGSKKRPFYRIVVADSRAPRDGRFIESLGTYNPLTTPKQVNFDEEAVMDWLQKGAQPSDTVRNMLQKAGVMKKFHEAKYSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK13</accession>
    <name>H4GK13_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endonuclease MutS2</fullName>
            <ecNumber evidence="7">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mutS2</name>
        <name evidence="10" type="ORF">PS3_8093</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Endonuclease that is involved in the suppression of homologous recombination and may therefore have a key role in the control of bacterial genetic diversity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DNA mismatch repair MutS family. MutS2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000054">
        <property type="protein sequence ID" value="EHS85865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122396.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000054.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8093"/>
    <dbReference type="EnsemblBacteria" id="EHS85865">
        <property type="protein sequence ID" value="EHS85865"/>
        <property type="gene ID" value="PS3_8093"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1239"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045910">
        <property type="term" value="P:negative regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03280">
        <property type="entry name" value="ABC_MutS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00092">
        <property type="entry name" value="MutS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005747">
        <property type="entry name" value="MutS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="PTHR11361"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF14">
        <property type="entry name" value="PTHR11361:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01713">
        <property type="entry name" value="Smr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005814">
        <property type="entry name" value="MutS_YshD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SSF160443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="SSF48334"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01069">
        <property type="entry name" value="mutS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Smr" evidence="9">
        <location>
            <begin position="715"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="515"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="334"/>
            <end position="341"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="789" mass="87566" checksum="FE9978AA91BEDC56" modified="2012-04-18" version="1">MNEKILSTLEYDAVKGLLEPFLVSAAGKRELGALVPQTDIKTIQDWLVETTDGADLLRVEGGIPIPTLADISPQLKRLKVDAALNGTELAQIMQVLRTSMRVQTFFEQLAEQKLTLRTLDQLVARIVTIPDVTKRLVRSLELDGRLKDEASTKLHGVRQLISQTETEIRQALEHYTRGKQAKKLSDPIITIRNDRYVIPVLAQYRNQFGGVVHDQSASGQTLYIEPGSVVELNNRLRQAQIEEVQEMRRVLIELSDLIRPYQDEIAQNEAILGHLDLINAKAKLAAQMKASLPIVNDQLVVNLRQARHPLIPQDQVVANDIQLGKDYQALVITGPNTGGKTITLKTLGIIQLMAQSGMFIPANENSQVTVFDNVFADIGDEQSLEQNLSTFSGHMENVKTILEQMTDHSLILLDELGAGTDPKEGAALAMAILDAIGTCGARVMVTTHYPELKVYAYDRQATINASMEFDQATLQPTYRLLIGIPGQSNGIAIANRLGLDPGIIQTAQSLVQADSQDLNQMIGDLVEQRKQAREENARLVELREANEAKEAELTQKLTRFDEQRDRLYDEARSKANHEVAQAKKQADKIIHHLRQLQVQQGANVKEHELINAKGQLNALHQDNPRLAHNSVLRRAKAKQALKPGDTVQVKSYGQQGTLVAKRGRHQWEVQLGILKMAIDEADLTKVAAGQIKTDAPKARKRPVRTIQTRQTAARLDLRGKRYEQAMSELDAFIDHALLNNLTSVTIVHGKGTGALRKGTQEYLRSNPRVKSFEFATPNNGGDGATIAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK16</accession>
    <name>H4GK16_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphoesterase</fullName>
            <ecNumber evidence="2">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_10196</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000054">
        <property type="protein sequence ID" value="EHS85868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122399.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000054.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10196"/>
    <dbReference type="EnsemblBacteria" id="EHS85868">
        <property type="protein sequence ID" value="EHS85868"/>
        <property type="gene ID" value="PS3_10196"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1242"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020935">
        <property type="entry name" value="PdiEstase_YfcE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="PTHR11124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01269">
        <property type="entry name" value="UPF0025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="172" mass="19102" checksum="DC7749DA95BA2429" modified="2012-04-18" version="1">MKVLLVSDNHGEGQILDRIVQIFGDQVDLMMHCGDSNLDPSTDVMTPFLTVEGNTDWGFTYPEIQRATVDHEKVVVTHGDHYSVNSTLTPLSLLAEAEAADVVGYGHTHQLAVTTADNHLLINPGSISQPRGEYAYIGGTFAIVEVTPAAWKVQYYNRDMQPIEELAFDWQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK18</accession>
    <name>H4GK18_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_8996</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000054">
        <property type="protein sequence ID" value="EHS85870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122401.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000054.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8996"/>
    <dbReference type="EnsemblBacteria" id="EHS85870">
        <property type="protein sequence ID" value="EHS85870"/>
        <property type="gene ID" value="PS3_8996"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1244"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009293">
        <property type="entry name" value="UPF0478"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06103">
        <property type="entry name" value="DUF948"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="37"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="145" mass="15830" checksum="4EB33253F170379F" modified="2012-04-18" version="1">MIIGQSMGSIAGLIAAGAFVVLVVFLCIFLTRMSAMLQETTRQITQLGNDADSLGRELEDILENTNRLMDDLNDKAEKITPAVEAIANVSQSVSDLNDASRAMVEKLNAQREQSPFKNRIFKTAAQAAMMGALGRFGKRKKHEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK21</accession>
    <name>H4GK21_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="3 10">6.3.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GARS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Glycinamide ribonucleotide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Phosphoribosylglycinamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">purD</name>
        <name evidence="13" type="ORF">PS3_7305</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-phospho-beta-D-ribosylamine + ATP + glycine = ADP + H(+) + N(1)-(5-phospho-beta-D-ribosyl)glycinamide + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the GARS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="3 10"/>
    <dbReference type="EMBL" id="AICN01000055">
        <property type="protein sequence ID" value="EHS85837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7305"/>
    <dbReference type="EnsemblBacteria" id="EHS85837">
        <property type="protein sequence ID" value="EHS85837"/>
        <property type="gene ID" value="PS3_7305"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1252"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00138">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020559">
        <property type="entry name" value="PRibGlycinamide_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472">
        <property type="entry name" value="PTHR43472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00184">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="12">
        <location>
            <begin position="111"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS85837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="418" mass="44802" checksum="ACEC8296B5DBE8C2" modified="2012-04-18" version="1">MEPVNVLVIGSGGREFAIAKKLKQSSHVDHVFCAPGNVGMPMVGVEPVAIEELDFPVLIDFAKSHQVAWTFVGPEDALCAGVVDAFQAAGLKIFGPNQRAAQLEGSKEYALDFMNQAGVPTAKHQAYADVEAALAGISEFGFPVVIKDNGLAGGKGVVIAEDQATAELTIREILKTPGSRLVLEECLIGPEYSMFVMIQDGEYRILPMAQDHKRANDGDKGPNTGGMGAYSPLPQLSAKDRQRMVDEVVVPTVQGLVAGDYHYHGILYIGLMLTADGPKVIEYNVRLGDPETQVILPRLATDLFDLVDACVNDQAMPEVEETNDAVLGVVLASAGYPVNPVHGQAIGPFPKQAGIEIDFANVGGSLDQLVGTGGRLLMVISKSATLEEAQHQVYQYLGQLDEPECFYRRDIGDKALEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK24</accession>
    <name>H4GK24_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="5">ATase</shortName>
            <ecNumber evidence="5">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
            <shortName evidence="5">GPATase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">purF</name>
        <name evidence="10" type="ORF">PS3_6178</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = 5-phospho-alpha-D-ribose 1-diphosphate + H2O + L-glutamine</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5 8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 5 6">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5 6">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000055">
        <property type="protein sequence ID" value="EHS85840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122407.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000055.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6178"/>
    <dbReference type="EnsemblBacteria" id="EHS85840">
        <property type="protein sequence ID" value="EHS85840"/>
        <property type="gene ID" value="PS3_6178"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1256"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 6 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5 6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="9">
        <location>
            <begin position="12"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5 7">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 8">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="484" mass="53465" checksum="8138C608E5351474" modified="2012-04-18" version="1">MPAEIKGLNEECGVFGVFGADHASQLTYMGLHALQHRGQEGAGIVTSNHERLFQHRDRGLLSKVFADPAELDLLQGDRAIGHVRYGTAGRNSVENVQPFLFRFHDGDTALAHNGNLVNAVSLKRELEDEGAVFQSDSDTEILIHLIRHYIKEDGFIPALKKSLNRVKGGFAFLLLQKDRLIAALDPNGFRPLCIGQMDNGAYVITSETCALDIVGAKFIRDVQPGELLVIDQDGLHEDHYTEDTQLAVCSMEYIYFARPDSIIHGVTVHTARKRMGKLLAQEHPIDGDIVIGVPNSSLSAASGYAEEIGLPYEMGLVKSQYVARTFIQPTQELRERSVQMKLAAVRGVVEGKRVIVVDDSIVRGTTSKQLIQMLRRVGAKEVHMVIASPPFKFPCFYGIDVSTRAELMAAHYSVEEMCQIIGADSLNFLSVPGLIKAIDMPDAGDAPYGGLTVAYFNGDYPTPLYDYEEGYRKSLSEQERKELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK39</accession>
    <name>H4GK39_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Response regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_22206</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000055">
        <property type="protein sequence ID" value="EHS85855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122422.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000055.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_22206"/>
    <dbReference type="EnsemblBacteria" id="EHS85855">
        <property type="protein sequence ID" value="EHS85855"/>
        <property type="gene ID" value="PS3_22206"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1273"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="5">
        <location>
            <begin position="134"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="201" mass="21991" checksum="3A5B98670D6CE3FB" modified="2012-04-18" version="1">MIKIYLAEDQTMLNSALKMILELEPDFTVVGTATDGEQACAEIKNLQPDVAILDIEMPKRSGLAVAQAICQQDLPIKIVILTTFARSNYFQTAIKAQVDGYLLKDSPSDSLISAIRSIMQGNTVFDPQLVAGVLHEVDNPLSKRELEVLQALEKTATTKELAAQLYLSEGTIRNYISAILSKTGTSSRLDAVNLAHQKGWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK41</accession>
    <name>H4GK41_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_21379</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000056">
        <property type="protein sequence ID" value="EHS85808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122424.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000056.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21379"/>
    <dbReference type="EnsemblBacteria" id="EHS85808">
        <property type="protein sequence ID" value="EHS85808"/>
        <property type="gene ID" value="PS3_21379"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1277"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="230" mass="25113" checksum="087FEA92D8D248C1" modified="2012-04-18" version="1">MISFKDVNKIYQVGDQEVHALSQASFTLEKGKLTIILGPSGSGKSTLLNILGGMDRPTNGTIDFDGEQVAQLSDTGLTNYRRQKVGFVFQFYNLIPSLTALENVAIAAKLNGNDAQSESYLEQVGLRHRLNNFPNQLSGGEMQRVSIARALAKKPQLLLCDEPTGALDSKTSLKIMALLQQAAQNPNTSVVMVTHNPTFEQYGDQVIRLKDGVIDRIDDNHSPVKLEGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK49</accession>
    <name>H4GK49_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Leucine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Leucyl-tRNA synthetase</fullName>
            <shortName evidence="9">LeuRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">leuS</name>
        <name evidence="15" type="ORF">PS3_8641</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000057">
        <property type="protein sequence ID" value="EHS85802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122432.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000057.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8641"/>
    <dbReference type="EnsemblBacteria" id="EHS85802">
        <property type="protein sequence ID" value="EHS85802"/>
        <property type="gene ID" value="PS3_8641"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1291"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_B">
        <property type="entry name" value="Leu_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002302">
        <property type="entry name" value="Leu-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025709">
        <property type="entry name" value="Leu_tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740">
        <property type="entry name" value="PTHR43740"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13603">
        <property type="entry name" value="tRNA-synt_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00985">
        <property type="entry name" value="TRNASYNTHLEU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00396">
        <property type="entry name" value="leuS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1g" evidence="13">
        <location>
            <begin position="39"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-synt_1_2" evidence="14">
        <location>
            <begin position="221"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-synt_1" evidence="11">
        <location>
            <begin position="417"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_1" evidence="12">
        <location>
            <begin position="659"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="9">
        <location>
            <begin position="580"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="583"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EHS85802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="808" mass="93028" checksum="F85B56DC5D3C554A" modified="2012-04-18" version="1">MGYDHSTIEKKWQRYWKQHKTFKADINKDQKKFYALDMFPYPSGQGLHVGHPEGYTATDVMSRMKRMQGYNVLHPMGWDAFGLPAEQYALKTGHNPADFTNQNINHFRDQIQALGFSYDWDREVNTTDPEYYKWTQWIFEQLYKKGLAYEDEIMVNWAPDFMGGTVVANEEVIDGKTERGGYPVYRVPMRQWVLKITAYADRLIDDLDLVNWPESVKEMQRNWIGRSVGASIKFQVADHADQEIEVFTTRPDTLFGLSYVVLAPELDLVNELVTPEQAAAVKEYQEQTSRRSDLERTDLNKDKTGVFTGAYAINPVNGQEVPIWISDYVLASYGTGAVMAVPAGDQRDWDFATKFGLPIPSIFEGHDTSEESEAFTGDAPHINSDFLNGLNNQASLDAMINWLEDHDCGGKKVNYRLRDWIFSRQRYWGEPIPVIHWDDGETTLVPEDELPLRLPKTDNLEPSGTGESPLANIEDWVNVYDENGRHGLRETNTMPQWAGSSWYWLRYTDPQNKDAFASKEALDYWSPVDLYVGGAEHAVLHLLYARFWHKVLYDLGYVPTPEPFMKLVNQGMILGANHEKMSKSKGNVVNPDDIVNQYGADTLRLYEMFMGPIEESVPWDEKGLHGSNKWLHRVWRLLMDENNHLRDRVSNYNDGKLTKIYNQTVKKVTEDFERMHYNTAISQLMVFVNEAYKVDDLPVEYMEGFVKMISPLVPHMAEEIWSHFHESDTIAYQPWPTYDENELVEDEVDIVVQVNGKVRAKIKMARDIDQQDAEDIALADDNVKAHVAGKEVVKVIVIPNRIVNIVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK76</accession>
    <name>H4GK76_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_12863</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nlpA lipoprotein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000061">
        <property type="protein sequence ID" value="EHS85637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122459.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000061.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12863"/>
    <dbReference type="EnsemblBacteria" id="EHS85637">
        <property type="protein sequence ID" value="EHS85637"/>
        <property type="gene ID" value="PS3_12863"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1328"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004872">
        <property type="entry name" value="Lipoprotein_NlpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30429">
        <property type="entry name" value="PTHR30429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03180">
        <property type="entry name" value="Lipoprotein_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002854">
        <property type="entry name" value="MetQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS85637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="281" mass="31204" checksum="FBEDA9DD54C06F6A" modified="2012-04-18" version="1">MSKKTRNWLIGIIILLVVVIGGYFSFVRPQQQAAKTVTIGIMSGTKQDDQLWDSVAKTAKDKYGITLKFKKFTDYNQPNKALTNGDIDLNAFQHKLFLKQWNKAHKTNIVAIGDTWITPIRLYSDKYKSVSDIPDGATIAVPNDATNESRALFALKNAGLFSLKKGTTLATVSDITNNTKNIKIKELSAEQVARSLSDVDAAVVNTNYANAAGLNYKDAIFVEPTNADSAQWVNVIAANKKDKNKQVYKDVVKSLQTAETKRLIKKFYGTAELPAWDMSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK80</accession>
    <name>H4GK80_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Sucrose-6-phosphate hydrolase</fullName>
            <ecNumber evidence="3 8">3.2.1.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 9">Invertase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PS3_16953</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Enables the bacterium to metabolize sucrose as a sole carbon source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.</text>
            <dbReference type="EC" id="3.2.1.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Glycan biosynthesis; sucrose metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyl hydrolase 32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.26" evidence="3 8"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122463.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16953"/>
    <dbReference type="EnsemblBacteria" id="EHS85532">
        <property type="protein sequence ID" value="EHS85532"/>
        <property type="gene ID" value="PS3_16953"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1338"/>
    <dbReference type="UniPathway" id="UPA00238"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004564">
        <property type="term" value="F:beta-fructofuranosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005985">
        <property type="term" value="P:sucrose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001362">
        <property type="entry name" value="Glyco_hydro_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013189">
        <property type="entry name" value="Glyco_hydro_32_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013148">
        <property type="entry name" value="Glyco_hydro_32_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006232">
        <property type="entry name" value="Suc6P_hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08244">
        <property type="entry name" value="Glyco_hydro_32C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00251">
        <property type="entry name" value="Glyco_hydro_32N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00640">
        <property type="entry name" value="Glyco_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01322">
        <property type="entry name" value="scrB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 8 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_32N" evidence="10">
        <location>
            <begin position="22"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_32C" evidence="11">
        <location>
            <begin position="331"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS85532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="472" mass="53985" checksum="4A3CE3C08C623212" modified="2012-04-18" version="1">MSLVKAKEPITVTNARYRQHYHLQTPGGWLNDPNGLCYFQGYYHVFYQYHPYSAEWGPMHWGHARSQDLLHWEDLPMALTPGDPEDTGGCFSGSAIVKDNRLYLIYTGHHYYDDGDQDHFWENQNVAYSDDGIHFTKYAGNPVIEAPDDNGQDFRDPKVWAHDGQYYLIVGSQAQPSRTGRVLLYQSADLFNWQLLGPITIAQSIDSEGFMWECPDLFHLNGQDTLVFSPMGIAAQGHDFLNLNQVASVMGNLDYSHHALNRGSLYEIDHGHNFYAPQSFLAPDGRQMMYGWMSSFDDPYPEQADGWCGCLTVPREYVIKNHHLGMIPVAELAQLRQQTIVSETIQLNHPVTIAYNDAQHAELDLTLEHPQNYQWRFRNQDQVIMSLTFDPEQNEFNLDRPHHGGKRYVTITPQPTHHLQIIIDTSSVEIFINDGEACFTERYYIDEAPSVILSAREPTKAQLAAYQLGIPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK90</accession>
    <name>H4GK90_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative citrate [pro-3S]-lyase ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_20955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20955"/>
    <dbReference type="EnsemblBacteria" id="EHS85542">
        <property type="protein sequence ID" value="EHS85542"/>
        <property type="gene ID" value="PS3_20955"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1350"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008771">
        <property type="term" value="F:[citrate (pro-3S)-lyase] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005216">
        <property type="entry name" value="Citrate_lyase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013166">
        <property type="entry name" value="Citrate_lyase_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40599">
        <property type="entry name" value="PTHR40599"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08218">
        <property type="entry name" value="Citrate_ly_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005751">
        <property type="entry name" value="Acet_citr_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00764">
        <property type="entry name" value="Citrate_ly_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00124">
        <property type="entry name" value="cit_ly_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Citrate_ly_lig" evidence="1">
        <location>
            <begin position="127"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="325" mass="36277" checksum="1666F4D99CF743C1" modified="2012-04-18" version="1">MEVNGIQHFEPSEVDQIEQTFVWIEGQEIAATGSLAGKVLKYIAVDRRYAAEGALFNQVISQLMQAAATNGQYHLFVFTKPQYQASFEYVGFHTLAATNDGVILETGTPDINSFIQAIPKPKAHQQVAAIVMNANPFTLGHRALVEAASRENDLVYLFIVQAERSLFTFQERWQLVKEGVADLTNVKPVASGDYLISAATFPAYFLKDDQDLGTYQAALDITLFKNQFVPALQIKRRYVGTEPLSKTTNSYNKVMKQLLPPEVEVVEMARKLDQTGQPISATRVRQAIATGDQQSLQALLPTSSYNYIKEHLATLQERMLKIGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK92</accession>
    <name>H4GK92_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Citrate lyase regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_14876</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SorC transcriptional regulatory family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14876"/>
    <dbReference type="EnsemblBacteria" id="EHS85544">
        <property type="protein sequence ID" value="EHS85544"/>
        <property type="gene ID" value="PS3_14876"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1352"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007324">
        <property type="entry name" value="Sugar-bd_dom_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04198">
        <property type="entry name" value="Sugar-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sugar-bind" evidence="2">
        <location>
            <begin position="41"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="296" mass="33454" checksum="FAAFD00757324408" modified="2012-04-18" version="1">MSINDISKKYNLSRYLIMKYLDEAINKQIVTINIHSGLDRNPELERRLHQEFAIKNLYVIKETDNTITQEKIISSFAATQVQAAIKPGTTVGIAWGETVADVITEFKQSVQPEITFTQFTGENMKYRSQAGSMRMVQNAAAKYNAPYYIIPAPLYIVSDQVRRQLVHEPAMERVFNVANQMDLVVCGLGTLASIDSIQPWRDHKAQIFPQVDLDQIAGMAYGRPFDLNGNFLNLGHDTTFSIPLETIRQVPRRFGIIKRKSKYQAALGALRGKLFTDLVITESIALLICHELETSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK95</accession>
    <name>H4GK95_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fumarate reductase flavoprotein subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_15357</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122478.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15357"/>
    <dbReference type="EnsemblBacteria" id="EHS85547">
        <property type="protein sequence ID" value="EHS85547"/>
        <property type="gene ID" value="PS3_15357"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1355"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010960">
        <property type="entry name" value="Flavocytochrome_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01813">
        <property type="entry name" value="flavo_cyto_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="2 4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="5">
        <location>
            <begin position="18"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS85547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="463" mass="50119" checksum="2FC1B67C9DA18CBC" modified="2012-04-18" version="1">MASEFQTTPHDQIKATYDAVIVGSGGTGLTAAIQAHELGLDVAVFEKNVELGGNTNRASSGMNAAETNVQLHHGVIDNVADFYRETYKDGGKLNDKDMLGYFVYHSAPAISWLADHGIKLDDITVTGGMSKKRTHRPASMAPIGGFLVKSLLNVAAQQNIPVFNETKVDQLLQDATGRVNGIKLSLDGHSQEVKAKAVILATGGFGASKAYMKRYRPDLIDYKTTNQPGATGDGLELAEEVGAELMQMDLIQVHPTVQQDHPHVYLIGEAVRGEGAILVNAQGQRFVNELNTRKIVSNAITALPEHSAYLIFDQGIRDRVKAIEFYDKTGLVEHGDTIEDLAQTLKMDPTVLKQTVRDWNQAVQDQNDEQFGRTTGMERDISQPGFFAIHIAPAIHYTMGGLHIDSKTHVLDGNGDVIEGLYAAGEVAGGLHGNNRVGGNSIAETIIFGRQAGQQVALWNSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK97</accession>
    <name>H4GK97_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="5">PPIase</shortName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_11344</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122480.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11344"/>
    <dbReference type="EnsemblBacteria" id="EHS85549">
        <property type="protein sequence ID" value="EHS85549"/>
        <property type="gene ID" value="PS3_11344"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1357"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000413">
        <property type="term" value="P:protein peptidyl-prolyl isomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PTHR45625"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="24"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="194" mass="21265" checksum="3AADD35E73C4009A" modified="2012-04-18" version="1">MSEFPQLSLEAANGPKATIKTNQGDITVQLFPDQAPKTVKNFVELAQQGYYDGVIFHRVIPEFMIQGGDPTGTGTGGESIYGAAFEDEFSNELFNFNGALSMANAGPNTNGSQFFIVTSTEMPASMIDQMRNAGYPEEVVIQYQQGGTPWLDHRHTVFGQVIDGMDVAVKISQVQRDYADKPVEDVVMETIEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKA6</accession>
    <name>H4GKA6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA methyltransferase, TrmH family, group 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_8636</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122489.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8636"/>
    <dbReference type="EnsemblBacteria" id="EHS85558">
        <property type="protein sequence ID" value="EHS85558"/>
        <property type="gene ID" value="PS3_8636"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1373"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004441">
        <property type="entry name" value="rRNA_MeTrfase_TrmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46429">
        <property type="entry name" value="PTHR46429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00186">
        <property type="entry name" value="rRNA_methyl_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SpoU_sub_bind" evidence="1">
        <location>
            <begin position="9"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="251" mass="27125" checksum="4F80E42596455B73" modified="2012-04-18" version="1">MTSEQTGEFIIGRHPAVAALKSDQAVNKVFIQKGLKSDALGQIIQLAKERQLIISNVPKAKLDQMTDHQNHQGVVLAVAAYQYAQIDDLFANAEAHQEAPFFLILDELADPHNLGSILRTADAAGVHGIIIPKRRSVGLTAVVAKTSTGAIEHVPVARVTNLVQTAKELQQRGVWLFGTDMQGTDYRQWQANGPVALVIGNEGKGISPLLKKTCDEMLTIPMIGHVQSLNASVAASLLIYQGFNSRHPSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKA8</accession>
    <name>H4GKA8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cysteine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cysteinyl-tRNA synthetase</fullName>
            <shortName evidence="11">CysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cysS</name>
        <name evidence="14" type="ORF">PS3_13765</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="11"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122491.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13765"/>
    <dbReference type="EnsemblBacteria" id="EHS85560">
        <property type="protein sequence ID" value="EHS85560"/>
        <property type="gene ID" value="PS3_13765"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1375"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015273">
        <property type="entry name" value="Cys-tRNA-synt_Ia_DALR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="PTHR10890"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09190">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00840">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11 14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DALR_2" evidence="13">
        <location>
            <begin position="356"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="307"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="11">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="11">
        <location>
            <begin position="271"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS85560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="469" mass="53570" checksum="66423CA0C5FB1E7B" modified="2012-04-18" version="1">MLKIYNTLSRQKEVFQPMTPGVIKMYVCGPTVYNYIHIGNARSIVAFDTVRRYFEFRGYEVQYVSNFTDVDDKMIKAAKEEGITVPQLADRYIQAFNEDTTALNVEPATDHPRATEYIPQIITFVQKLIDNGYAYVVDGDVYYRARHFKNYGALSGQSIDDLETGASEHVDPEEVAKKEDPLDFALWKAAKPGEINWESPWGKGRPGWHIECSVMSTDHFGPTFDIHAGGQDLQFPHHENEIAQSEAATGEKFVNYWMHNGFVTIGQDNEKMSKSLGNFVTVHDLLKAEDPQVIRFFMATTHYRRPIQYSQANLDEARANLEHLRNTFHNLQYRLKDATSGTDGDVQRQLAQFRDRFTEVMDDDINVANGIAVVYELMKFANQYVEQAAVQREPLVAIQVMLKKLLQVFGIEISLEAQSQDQEIEALIKQRDAARAAKDFALSDQIRDTLKARGVIIEDTPQGTRYRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKB2</accession>
    <name>H4GKB2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribose-5-phosphate isomerase A</fullName>
            <ecNumber evidence="2">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Phosphoriboisomerase A</fullName>
            <shortName evidence="2">PRI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpiA</name>
        <name evidence="3" type="ORF">PS3_22102</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122495.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_22102"/>
    <dbReference type="EnsemblBacteria" id="EHS85564">
        <property type="protein sequence ID" value="EHS85564"/>
        <property type="gene ID" value="PS3_22102"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1381"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00170">
        <property type="entry name" value="Rib_5P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020672">
        <property type="entry name" value="Ribose5P_isomerase_typA_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="28"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="84"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="97"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="229" mass="25153" checksum="BED6EEEBC21BECC6" modified="2012-04-18" version="1">MTQDELKKMVGLAALPFIKPGMKIGLGTGSTVKHLVDGLGKRMREEGLQVTCVTTSSITTRQANELGIPLVGLDDVDHLDLTIDGADQVDENFNGIKGGGGSMLWEKIVNTASDQNIWIVDESKLTERIGSFGVPVEIVPYGASHVFRRLEQKGLKPTWRLAKDSDQLYRTDENNVIIDVHPGPIDDVNALDQELINMVGVVEHGLFIHRINDIIIGREQGPETIHVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKB8</accession>
    <name>H4GKB8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Malate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_5739</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the auxin efflux carrier (TC 2.A.69) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122501.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5739"/>
    <dbReference type="EnsemblBacteria" id="EHS85570">
        <property type="protein sequence ID" value="EHS85570"/>
        <property type="gene ID" value="PS3_5739"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1389"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="289"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="316" mass="34813" checksum="65C3B56841EC1C77" modified="2012-04-18" version="1">MVTVFLSALSGVSVIIIMVVAGFVMNERGWFSPTSSKTISRIVTQISLPAYMISTITKDFTAGKLSILLPDLRYPVISMLIMFALSFAVARALAIKRSHIGLFSSMFFNSNTVFIGLPINLALFGDASIPYVLVYYMANTTFFWTLGVWLIQRDGMKDVKIDVKQALKKVFSAPLLGFIIGVALVLLKIHLPKFILQTCGYLGNLTIPMSMIFIGIAISNAGLKRVRFNRDAWGILFGRFLFAPIIMTLLVLPSSMPVLMKQVFILQSAMPVMTNAPVVSKMYNADDTYASIMVAETTVISVIVIPVLMVIIKSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKE5</accession>
    <name>H4GKE5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UDP-N-acetylglucosamine kinase</fullName>
            <ecNumber evidence="3">2.7.1.176</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">UDP-N-acetylglucosamine kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_7113</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + UDP-N-acetyl-alpha-D-glucosamine = ADP + H(+) + UDP-N-acetyl-alpha-D-glucosamine 3'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:32671"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:64353"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.176"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zeta toxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.176" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000064">
        <property type="protein sequence ID" value="EHS85437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122528.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000064.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS85437">
        <property type="protein sequence ID" value="EHS85437"/>
        <property type="gene ID" value="PS3_7113"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1447"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010488">
        <property type="entry name" value="Zeta_toxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06414">
        <property type="entry name" value="Zeta_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="domain" description="Zeta_toxin" evidence="9">
        <location>
            <begin position="4"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="226" mass="25989" checksum="78B9956B1FA5CEB5" modified="2012-04-18" version="1">MIDKPQYIIVAGVNGAGKSTLYDTFPSLFDKTKRINADELLKQMGGDWHKDSDNLKAMKEEIKQLHYALDHQQSIHVETTLAGRGKAQLNLIDKAHKNGFEVTLLYVALRDENLAIQRVNERVQKGGHGIPVATIKKRYQQSKHNLPLVAFKSDKVMIYDNSEKFTSVYAREKGQVFKNDLRYFPWISQSITYPEKVQKQLQNFADQNPEVKPKNNPENKNDRPSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKH4</accession>
    <name>H4GKH4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_10568</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000067">
        <property type="protein sequence ID" value="EHS85388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122556.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000067.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10568"/>
    <dbReference type="EnsemblBacteria" id="EHS85388">
        <property type="protein sequence ID" value="EHS85388"/>
        <property type="gene ID" value="PS3_10568"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1510"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS85388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="276" mass="29003" checksum="61D5D7DF4B6BEFD2" modified="2012-04-18" version="1">MLKQPKEIIILLGAGQMGLKIVQRLASNHTILLGDISEDNLARAKEGLLDAGFVVETKIVDGSQRESIQAFADYAQSLGEVTNYIHTAGVSPNMANPQPIIDVDLVGTAIALEIFRDVIAPGGAGLVISSQAGHMFPLDPELEQQLATVPADQLAALPGVKAIDNSGLAYGVAKRANIVRVQHEAVLWGDHGARLNTVSPGVIITPLARHELTAQPEAYGEMIETSPAGRPGTPDEVAAAAEFLLDPKNSFITGTDLLIDGGVIAAMATGRYQLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKH8</accession>
    <name>H4GKH8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ThiW-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_11233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000067">
        <property type="protein sequence ID" value="EHS85392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122560.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000067.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11233"/>
    <dbReference type="EnsemblBacteria" id="EHS85392">
        <property type="protein sequence ID" value="EHS85392"/>
        <property type="gene ID" value="PS3_11233"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1519"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012652">
        <property type="entry name" value="ThiW"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09512">
        <property type="entry name" value="ThiW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF024534">
        <property type="entry name" value="ThiW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02359">
        <property type="entry name" value="thiW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="171" mass="18447" checksum="B7C3A00A8F347E44" modified="2012-04-18" version="1">MNYQHQHIYRLTLTAILVALATCGASLFSFPIGVAKVAPVQHFINVLSGILLGPEWALLQACLTSLLRNLLGTGTILAFPGSMIGAFLSGWFYQHTKKLWAGVSGEIVGTGIIGAIIARPVAALFMGTKGAIWIFVPSFFLSTLVGSLVAYLFLKAAWPLINRCLIKQQED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKI8</accession>
    <name>H4GKI8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP-dependent DNA helicase/translocase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_8839</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122570.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8839"/>
    <dbReference type="EnsemblBacteria" id="EHS85268">
        <property type="protein sequence ID" value="EHS85268"/>
        <property type="gene ID" value="PS3_8839"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1532"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="110"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="293"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="433" mass="49537" checksum="179EE80F41E8E3C3" modified="2012-04-18" version="1">MEVSHYYGRQVLAYPCEMDNLPSEIQIIDPIEIRSTTIRCRRCNQIIKKPDAALPHHQYYCPACINLGRVSTLAKFYHLPEPNQFEIPPTVLTWQGQLSPLQKMAAQEVAASMAHHDHRLLWAVTGAGKTEMIFPGLAAAIKRGERLAIASPRVDVCLELYPRLQEAFANTKIALLHGRQPEQYHYRQLTICTTHQLLRFYQAFDNLIIDEVDAFPYASNEQLIYATHQAIKPTGGLLYLTATPGPALFKQIKKHQLEVSYLPLRYHGHLLPEIKVKRAGRWRHRLPKSLIQWIKQRLGKLEMLIFVPHVADLAYVEAIFKEQIHQARFTTVHAADPQRLEKVQAMRDHQYDFLITTTILERGVTFPKIDVAVLGADDQVFSASALVQIAGRVGRSSERPGGDVTFWVQEPAVNVKEAQRQIKQMNRRGRHYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKJ7</accession>
    <name>H4GKJ7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ATP-dependent RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_4094</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122579.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4094"/>
    <dbReference type="EnsemblBacteria" id="EHS85277">
        <property type="protein sequence ID" value="EHS85277"/>
        <property type="gene ID" value="PS3_4094"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1542"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="27"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="224"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="374"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="403"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="425" mass="47824" checksum="5D002DDA8498F2DB" modified="2012-04-18" version="1">MQLNDRFQQHFQERGFEEPTLIQEQVFQPLLQDQDLLGIAPTGSGKTLAFAWPLLPKLMPQQGVQLVVLEPSQELAIQTSQVLREWGSLLGLRIQSLTGGANLKRQAERLKKGHPEVVVGTPGRVNHLLDERILKLHRVIAVVVDEADELLTDDSHDQVLRVIQVVPQETQLALFSATSGELIEQLRDLFDRDFSVVDVRADDHSRGPVNHGLVKASTMSDKAGMLERLSHTKNFRAFVFFTSIKTLHYTASRLQHAGVPVASLGGKQRQTEREEMLRKFRKRQVKLLLTTDVAARGLDIPKLPAVVNFELPNSADAYVHRTGRTGRQGEPGTVINLGNDHDFRDLKKLLAETDYVLKPMTIAKQRVMDQEKVKELVQSSSKDKSTSVTKDPKGSTTQSTPLKARKKKNRKNKGIRLKHRRKAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKK2</accession>
    <name>H4GKK2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_20410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000070">
        <property type="protein sequence ID" value="EHS85229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122584.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000070.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20410"/>
    <dbReference type="EnsemblBacteria" id="EHS85229">
        <property type="protein sequence ID" value="EHS85229"/>
        <property type="gene ID" value="PS3_20410"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1550"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000150">
        <property type="entry name" value="Cof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00099">
        <property type="entry name" value="Cof-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS85229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="261" mass="29200" checksum="C7D2933252B9B16F" modified="2012-04-18" version="1">MKYFISDLDHTLLNEQAKLSLETIRVIKSNQLPLMLCSARLPKQMDPFIQQLNLTGPQAALNGALIFEGIGEDRRIQAKHAISLSIYQQIKELMRKKFPDYPVTWMDEEKWYLPRLTADLTPELQYCQADDYIIGMNQPLNDPLLLLIVIPDANQFKAVFTCLQQTAIPEIQVVNSGDGYLTITGQGVNKGLAPQYLLEHHLAVPKEIVAVGDDENDLPMFKRAGISVAVANAAPSVRMQADIVGDTNENDGVARIMERLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKL5</accession>
    <name>H4GKL5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Diadenylate cyclase</fullName>
            <shortName evidence="10">DAC</shortName>
            <ecNumber evidence="10">2.7.7.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cyclic-di-AMP synthase</fullName>
            <shortName evidence="10">c-di-AMP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dacA</name>
        <name evidence="12" type="ORF">PS3_4590</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP), a second messenger used to regulate differing processes in different bacteria.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>2 ATP = 3',3'-c-di-AMP + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:35655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:71500"/>
            <dbReference type="EC" id="2.7.7.85"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Probably a homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the adenylate cyclase family. DacA/CdaA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.85" evidence="10"/>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122597.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4590"/>
    <dbReference type="EnsemblBacteria" id="EHS85184">
        <property type="protein sequence ID" value="EHS85184"/>
        <property type="gene ID" value="PS3_4590"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1572"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106408">
        <property type="term" value="F:diadenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019932">
        <property type="term" value="P:second-messenger-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01499">
        <property type="entry name" value="DacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014046">
        <property type="entry name" value="C-di-AMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034701">
        <property type="entry name" value="CdaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045585">
        <property type="entry name" value="CdaA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036888">
        <property type="entry name" value="DNA_integrity_DisA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003390">
        <property type="entry name" value="DNA_integrity_scan_DisA_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19293">
        <property type="entry name" value="CdaA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02457">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004793">
        <property type="entry name" value="UCP004793"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143597">
        <property type="entry name" value="SSF143597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00159">
        <property type="entry name" value="TIGR00159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51794">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="DAC" evidence="11">
        <location>
            <begin position="83"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS85184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="281" mass="31730" checksum="1A95C26302FF19F5" modified="2012-04-18" version="1">MDWLASLLTWHNLASLIDILLIWFFIYELIVLIQGTRAVQLFKGILVIIVLKIISWYCGLSTVSWVMDQVINWGVIAIVVIFQPEIRRGLEHIGRGSFFTRNRTENEAEEHMITQIDQAIQYMSKRRIGALITIEMGTGLEEYIETGIPMDADVTSALLINTFIPNTPLHDGAVIIKDGRIAVAAAYLPLSDSKLIPKELGTRHRAAVGISEVTDALTIVISEETGEVSFTKDNELLRGMTRDNYLKFMRANLYRSESQPVSNLLTRFLMLLRGKGGRHEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKM9</accession>
    <name>H4GKM9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="2 6">6.3.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_6383</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 6">
            <text>ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-cysteine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glutamate--cysteine ligase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="2 6"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122611.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6383"/>
    <dbReference type="EnsemblBacteria" id="EHS84949">
        <property type="protein sequence ID" value="EHS84949"/>
        <property type="gene ID" value="PS3_6383"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1589"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007370">
        <property type="entry name" value="Glu_cys_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006334">
        <property type="entry name" value="Glut_cys_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38761">
        <property type="entry name" value="PTHR38761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04262">
        <property type="entry name" value="Glu_cys_ligase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glu_cys_ligase" evidence="7">
        <location>
            <begin position="18"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Glu_cys_ligase" evidence="7">
        <location>
            <begin position="233"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="455" mass="51871" checksum="5B3F25B7B8C6E1C5" modified="2012-04-18" version="1">MFSRIGQIIFDHEAVLEASDFKMGIEIEMNRVDKRGHLSREPYPAAVGDQAQNPWLTNDFLSTMSEIVTPAADNATDAMHYLYSINTALRTALAPGEYLWPMSMPPILPDDPHDLPIAATTPEKREYFDHWLERHQPSEGTPQGAHINLSINQNVIKLLSQELDLSEKAVKNHLYKTLAQGFIRYRWVITYLFGASPVAEANFYGPDQQPFDHPVRSIRQSHRYGLGNDFIADYTSVETYAQKIQEAVDAGELLKPTDYHGSVRFKGADEMKDLAKEGAKYLELRMLDLDPTSSIGIRTGTIRFIRLLAGYFLMSPAINDRDVNDVLKRADQMNGEVAEEMPTERTQYYEEARAFIERLQQFVNQIQAGPEYQEILYDFEYRINHPETTPSGRLVEKLHNGSLVDYAMRKAIKYQEGAQQALKPFHGFEENNGNLSADLLAKELFGGSWRVNYHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKP5</accession>
    <name>H4GKP5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Urocanate reductase</fullName>
            <ecNumber evidence="3">1.3.99.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_4080</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>A + dihydrourocanate = AH2 + urocanate</text>
            <dbReference type="Rhea" id="RHEA:36059"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:27247"/>
            <dbReference type="ChEBI" id="CHEBI:72991"/>
            <dbReference type="EC" id="1.3.99.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="3">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="3">Binds 1 FMN covalently per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.33" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122627.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4080"/>
    <dbReference type="EnsemblBacteria" id="EHS84965">
        <property type="protein sequence ID" value="EHS84965"/>
        <property type="gene ID" value="PS3_4080"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1615"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010960">
        <property type="entry name" value="Flavocytochrome_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007329">
        <property type="entry name" value="FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04205">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00900">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01813">
        <property type="entry name" value="flavo_cyto_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="1 3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_bind" evidence="4">
        <location>
            <begin position="12"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU366062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS84965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="626" mass="66908" checksum="0F8203F1FC6749F9" modified="2012-04-18" version="1">MKAGTYHVKAKGHGSSFMPMEVTIDQDRIVSVQVDAKGETKGVADKVFKRVPQAIVDGQTLNVDTVAGATISSHGVIDGVAQAIELAGGDPAQWQTKAVNNSQAQDQELTTDVVVVGAGGAGLAAAVKTVELGHQVVVLEKFPQIGGNTTRTGGPLNAADPAWQKEFHDLPGEKETLTELMNLSVDEIDPEYQADFRQLQAQIKEYLASGADYLFDSVLLHEIQTYLGGKRTDLKGNTIYGKYPLVHKLVANVLDSVHWLQKLGVEFDQTEVTEPVGGLWRRGHKPVEPMGCAFIHVLGDWLAQRQVPVLTETKAQHLLMDHGQVVGVQAQKVDGSQVTVHAKAVILTAGGFGANTKMVQQYNTYWSAIDDDIATTNSPAITGDGIQLGTEAGADLVGMGFIQLMPVSDPVTGELFTGLQTPPANFIMVNQAGKRFVNEFAERDTLAKAAIDNGGLFYLIADDKIKETAYNTSQESIDAQVAAGTLYRDDTLAGLAKQVGMDPATLVETIDKYNTYVEQENDPEFHKNVFGLKCVQAPFYATPRKPAIHHTMGGLVIDDLAHVLNGQGQVISGLYSAGENAGGLHAGNRLGGNSLADIFTFGRIAAETAVSDKVAVDTNASASVHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKP6</accession>
    <name>H4GKP6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Riboflavin biosynthesis protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="11">Riboflavin kinase</fullName>
                <ecNumber evidence="11">2.7.1.26</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="11">Flavokinase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="11">FMN adenylyltransferase</fullName>
                <ecNumber evidence="11">2.7.7.2</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="11">FAD pyrophosphorylase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="11">FAD synthase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PS3_7137</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 11">
            <text>ATP + FMN + H(+) = diphosphate + FAD</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + riboflavin = ADP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14357"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 11">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the ribF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.26" evidence="11"/>
    <dbReference type="EC" id="2.7.7.2" evidence="11"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122628.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7137"/>
    <dbReference type="EnsemblBacteria" id="EHS84966">
        <property type="protein sequence ID" value="EHS84966"/>
        <property type="gene ID" value="PS3_7137"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1616"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00406"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00277">
        <property type="reaction ID" value="UER00407"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02064">
        <property type="entry name" value="FAD_synthetase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015864">
        <property type="entry name" value="FAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023468">
        <property type="entry name" value="Riboflavin_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002606">
        <property type="entry name" value="Riboflavin_kinase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015865">
        <property type="entry name" value="Riboflavin_kinase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22749">
        <property type="entry name" value="PTHR22749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06574">
        <property type="entry name" value="FAD_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01687">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004491">
        <property type="entry name" value="FAD_Synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00904">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="SSF82114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00083">
        <property type="entry name" value="ribF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 11" id="KW-0288">FMN</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Flavokinase" evidence="12">
        <location>
            <begin position="187"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR004491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS84966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="313" mass="35881" checksum="9FBE5F9135BFA2A5" modified="2012-04-18" version="1">MQVVHLHYPINLQRVAKNPLVLAMGFFDGVHRGHQAVIQRAKQEAEYRKLPLAVLTYDKFPGIVYQSIPGRFRYLTTLERKVELLEQLGVDLVYVMDFTSHLGALRPQEFVDEILLKMHVKVVVAGFDHTYGDHQVATMERLANFAQGRFEVVTVDKFIDDQSSEKISSTRIRQAIDEGLVEQANELLGYHYQSHGIVVHGFARGRTIGFPTANVQWKIDERLPAVGVYAVRFRVNQVWYQGMASVGYNVTFGDNHEKTIEVFLFDFNDQIYGEQVTVEWIQRLRGEIKFAGVEELVAQLQQDEVDSRQILAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKP7</accession>
    <name>H4GKP7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA pseudouridine synthase B</fullName>
            <ecNumber evidence="4">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridine(55) synthase</fullName>
            <shortName evidence="4">Psi55 synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">truB</name>
        <name evidence="7" type="ORF">PS3_12878</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12878"/>
    <dbReference type="EnsemblBacteria" id="EHS84967">
        <property type="protein sequence ID" value="EHS84967"/>
        <property type="gene ID" value="PS3_12878"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1617"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02573">
        <property type="entry name" value="PseudoU_synth_EcTruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01080">
        <property type="entry name" value="TruB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014780">
        <property type="entry name" value="tRNA_psdUridine_synth_TruB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767">
        <property type="entry name" value="PTHR13767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00431">
        <property type="entry name" value="TruB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TruB_N" evidence="5">
        <location>
            <begin position="29"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="TruB_C_2" evidence="6">
        <location>
            <begin position="185"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS84967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="305" mass="34005" checksum="8251FB5BD9DC2243" modified="2012-04-18" version="1">MNGGVQMDGIIPLYKERGMTSFDCVSRLRRILHTKKIGHSGTLDPNVDGVLPICVGNGTKVVEYLMNSGKEYVGEVLIGQSTTTEDLDGELVETKAVPQAIPAAQIQAVMEQMTGQITQIPPMYSAVKVKGKRLYEYARAGETVERPQRQVTISSFRLISTDYDESSQTQRARFVVACSKGTYVRTLAVDLAKQLGYPGVMSQLTRKKSGGFEIDQTLSLADIQDMVSEEMMGRYIYPVDYALKDLPHHELSTDQWSGVQNGAWLKPSELSTDAKQVVLTYHDDAKAIYQYRDQVYKPEKMISTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKT1</accession>
    <name>H4GKT1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">PTS system cellobiose-specific IIC component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_5721</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122663.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5721"/>
    <dbReference type="EnsemblBacteria" id="EHS85001">
        <property type="protein sequence ID" value="EHS85001"/>
        <property type="gene ID" value="PS3_5721"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1659"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003352">
        <property type="entry name" value="PTS_EIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004501">
        <property type="entry name" value="PTS_EIIC_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02378">
        <property type="entry name" value="PTS_EIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51105">
        <property type="entry name" value="PTS_EIIC_TYPE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="321"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="345"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="395"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIC type-3" evidence="9">
        <location>
            <begin position="1"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="436" mass="49059" checksum="72CD2060720265C5" modified="2012-04-18" version="1">MKKRILDLAIAYRRKSFIRVAYRTFATLFPFVLIGIWIQIINTNLLQPDGFLYNVLYLANWTSTTWLRPLRLVATFIQNILLGFIGIYVVYLAAYYTAQLYQRAKQLAGVTGIVVMLMASYQASNRTGNFYSSLNWRMLSLQNLLLSLAIGYLIGQVFRWLSPATSATHNPHTLEIQSRAQWNLKPIIVSLIIGIVLGVGMNLANYFAISDSVITALSDWGAGDSHFIIKLLVMMLISLLQWLGLYDPTSTWFTTGDSAEWNANLSYVLSHHSMYHVPYPLSGVELYSSYAVFGGVGCTLALLIAFVLVSRRLENLRVARWNMIPVLFNNSGAFFIGLPVILNPIFLLAMIIIPAINMLIAAGAVMIHLIPTPVFDYPNGTPTFLVSFIGTNGNWASVIFAILLFCLDIIMYLPVVRIYIEMQNEIIKLDLKEDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKT2</accession>
    <name>H4GKT2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_6155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122664.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6155"/>
    <dbReference type="EnsemblBacteria" id="EHS85002">
        <property type="protein sequence ID" value="EHS85002"/>
        <property type="gene ID" value="PS3_6155"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1660"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011152">
        <property type="entry name" value="Pesterase_MJ0912"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000883">
        <property type="entry name" value="Pesterase_MJ0912"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="2">
        <location>
            <begin position="4"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="280" mass="32478" checksum="4EE65177AD3A7788" modified="2012-04-18" version="1">MKQKIAVLADVHGNLTALQAMYEDSQKQGVDQYWFLGDLLMPGPGVMEVWHLLQEMQPSLCVRGNWDDLAIHTSRGEGYLTKPSRIFFARLGQYVAEQGGASAVKIMEKWPLHQTVTVGPLNFALSHNLPDKNLGQALFPTNDVTNFDQLLTDLQVDIAIYAHVHHQLLRYGSDERIILNPGSVGEPFNHHEKLQRDLRAYYLIMEIDDYGLASLNYRHVYYDREVEYQRAVVAKLPYLELYRRMIDTGRVDTHNQPLLDQMNQKYRYREQYQAFQKSLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKT6</accession>
    <name>H4GKT6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipoate--protein ligase</fullName>
            <ecNumber evidence="4">6.3.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_14484</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-lipoate + ATP + L-lysyl-[lipoyl-carrier protein] = (R)-N(6)-lipoyl-L-lysyl-[lipoyl-carrier protein] + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10500"/>
            <dbReference type="Rhea" id="RHEA-COMP:10502"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 2/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.20" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122668.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14484"/>
    <dbReference type="EnsemblBacteria" id="EHS85006">
        <property type="protein sequence ID" value="EHS85006"/>
        <property type="gene ID" value="PS3_14484"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1664"/>
    <dbReference type="UniPathway" id="UPA00537">
        <property type="reaction ID" value="UER00594"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016979">
        <property type="term" value="F:lipoate-protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019491">
        <property type="entry name" value="Lipoate_protein_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004562">
        <property type="entry name" value="LipoylTrfase_LipoateP_Ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12561">
        <property type="entry name" value="PTHR12561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10437">
        <property type="entry name" value="Lip_prot_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00545">
        <property type="entry name" value="lipoyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="8">
        <location>
            <begin position="26"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS85006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="339" mass="38803" checksum="904B2104E6E45F4A" modified="2012-04-18" version="1">MQYVAMKSHDIRRNLATEQYLMNSDQIKPPFMLFYIEDSCIIVGRNQNTLEEINQKYCEEHHVTITRRLSGGGAMYQDMGNMCFSFVVPADQVKFGDFGELVKPVVDALHDMGATGAEVNGRNDITIDGKKFSGNAMYSRHGKTFSHGTLMYNVNTDTVADALQVPKDKIESKGIKSVRSRVTNIRPYLRPEFQNLTTEEFRDELIKRIWKVDSIEDAQVNEYQLTAADESAIDEIENKWYKNWDWVYGRSPEFTVKKRRHFTSGTIDARFLIEHGKIANLKIYGDFFGTGDVHEVEEALTGVEYSPEAMTKVLNQLDLQKYFAGSDTDEVIQLLTIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKV3</accession>
    <name>H4GKV3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Imidazole glycerol phosphate synthase subunit HisH</fullName>
            <ecNumber evidence="8">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">IGP synthase glutaminase subunit</fullName>
            <ecNumber evidence="8">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">IGP synthase subunit HisH</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">ImGP synthase subunit HisH</fullName>
            <shortName evidence="8">IGPS subunit HisH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hisH</name>
        <name evidence="11" type="ORF">PS3_10979</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="8"/>
    <dbReference type="EC" id="3.5.1.2" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122685.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10979"/>
    <dbReference type="EnsemblBacteria" id="EHS85023">
        <property type="protein sequence ID" value="EHS85023"/>
        <property type="gene ID" value="PS3_10979"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1685"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00278">
        <property type="entry name" value="HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42701">
        <property type="entry name" value="PTHR42701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000495">
        <property type="entry name" value="Amidotransf_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6 8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GATase" evidence="10">
        <location>
            <begin position="4"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 9">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="active site" evidence="8 9">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="active site" evidence="8 9">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000495-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS85023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="202" mass="22019" checksum="B4099F489C1190DB" modified="2012-04-18" version="1">MIAIIDYGAGNTFNVQKAFDFLGVETVLTSDPKVIDQSSALVLPGVGAFKAAMDNLQAAGLVDIIKTTVANGKPLLGICLGMQMLFEYSEEYGQTTGLGLIPGKVVAIPDGDLLVPQMGWNQQILHKPTSVFNEVANQYTYFVHSYYAQTDPQYITSTVDYGVQVPAIVERDQIIGMQFHPEKSGQVGLRLLKDFVEKVVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKV5</accession>
    <name>H4GKV5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histidinol dehydrogenase</fullName>
            <shortName evidence="4">HDH</shortName>
            <ecNumber evidence="4">1.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hisD</name>
        <name evidence="11" type="ORF">PS3_9700</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>H2O + L-histidinol + 2 NAD(+) = 3 H(+) + L-histidine + 2 NADH</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4 9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5 10">Belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.23" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122687.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9700"/>
    <dbReference type="EnsemblBacteria" id="EHS85025">
        <property type="protein sequence ID" value="EHS85025"/>
        <property type="gene ID" value="PS3_9700"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1687"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00014"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01024">
        <property type="entry name" value="HisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001692">
        <property type="entry name" value="Histidinol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022695">
        <property type="entry name" value="Histidinol_DH_monofunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="PTHR21256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000099">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00611">
        <property type="entry name" value="HISOL_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="1 4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0520">NAD</keyword>
    <keyword evidence="3 4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 9" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="4 6">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4 6">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 7">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 7">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 7">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8 9">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 8 9">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 8">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8 9">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 8">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8 9">
        <location>
            <position position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS85025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="425" mass="45812" checksum="CC09C66E24332A5C" modified="2012-04-18" version="1">MKIYQEDLATMKRIVDDYTQETVDFEIEQSVQTIIQNVIKNGDQALKDYSKKFDQVDLDKLLVEPSDLEAAYATIDDDLKAALLTAKDNITAFHELEKETGFLDVSTPGIVRGQKLTALKRVGLYVPGGTAAYPSTILMNAIPAKIAGVQELVMITPPQKDGINPAVLAAAKIGGVDAVYQVGGAQGIAALAYGTETIPAVDKIVGPGNIFVATAKKQVFGQVAIDMVAGPSEIGILADANAKPSELAADLLSQAEHDRRARAMLVTDSLDLAQAVSKEVDQQLTTLPRQEIAQAAINDRSFIAVMPKVTEMFELMNAIAPEHLEIAFDNPSQYLGLVENAGSVFLGRYSTEPLGDYLAGPNHVLPTGGTARFSSPLGVYDFVKRTQFLQYSAEQLANVANKVTKFARTEGLEAHARAIEARYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKW2</accession>
    <name>H4GKW2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13762</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122694.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13762"/>
    <dbReference type="EnsemblBacteria" id="EHS85032">
        <property type="protein sequence ID" value="EHS85032"/>
        <property type="gene ID" value="PS3_13762"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1696"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020215">
        <property type="entry name" value="EbsA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17255">
        <property type="entry name" value="EbsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="134" mass="15666" checksum="5712D6309132F77F" modified="2012-04-18" version="1">MTEKKKYFYQPDITTAIIAWCWTFLILIVGVVIWLEITTFNWITAVFLLVFLVISALAIFRRTMIVTEDRLILNRVLQQDYAEIPLSAIKHLYFRKNTVVFTAHGEVMTFSMSRRSIRSLETLLNNLMAKSKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKW7</accession>
    <name>H4GKW7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Holliday junction resolvase RecU</fullName>
            <ecNumber evidence="13">3.1.21.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Recombination protein U homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">recU</name>
        <name evidence="15" type="ORF">PS3_1805</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Endonuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves mobile four-strand junctions by introducing symmetrical nicks in paired strands. Promotes annealing of linear ssDNA with homologous dsDNA. Required for DNA repair, homologous recombination and chromosome segregation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 13">Belongs to the RecU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.10" evidence="13"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122699.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_1805"/>
    <dbReference type="EnsemblBacteria" id="EHS85037">
        <property type="protein sequence ID" value="EHS85037"/>
        <property type="gene ID" value="PS3_1805"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1703"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00130">
        <property type="entry name" value="RecU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004612">
        <property type="entry name" value="Resolv_RecU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03838">
        <property type="entry name" value="RecU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037785">
        <property type="entry name" value="RecU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00648">
        <property type="entry name" value="recU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 13" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 13" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 13" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="13">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EHS85037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="207" mass="23662" checksum="85B641418464229E" modified="2012-04-18" version="1">MAIKYPQGQQPPRPAPEQPQLGSSAAKRGMTLEHELNLSNQYYLSVDRAVIHKKPTPIQLVKVDYPRRSAAVVREAYFRRPSTTDYNGIYQGHYIDFDAKETRNKRSFPLKNFHAHQIEHLRHCLKQGGICFAIIRFTNLGQNYLYPASKLIQAWDQQDAGGRKSILLTTIQTDGFPITLGLQPRLDYLAAVDQLLETIKHKGVDYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL07</accession>
    <name>H4GL07_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NhaC family sodium:proton (Na+:H+) antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_5591</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122739.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5591"/>
    <dbReference type="EnsemblBacteria" id="EHS84773">
        <property type="protein sequence ID" value="EHS84773"/>
        <property type="gene ID" value="PS3_5591"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1755"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018461">
        <property type="entry name" value="Na/H_Antiport_NhaC-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03553">
        <property type="entry name" value="Na_H_antiporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="408"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Na_H_antiporter" evidence="7">
        <location>
            <begin position="174"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="463" mass="49116" checksum="3B6D63C355684F93" modified="2012-04-18" version="1">MKHAHATNRPEPKFGFGESIMILIAILIILGSLIIVKQQEPQAPLFIAFTLLAVYARLRGFKWSTIMDGMRTGLKDGVDPLVIFLSIGVLIATWIFSGTIPTIMYFGFKIISVQFFLPTVFIVCTLVGVACGSSFTTVSTMGIAFVGIGATLKINPGLTAGVIVSGAFCGSNISPLSGTTNLAASVGEINIYKHIKALMITDIPAWAICVVIYTLLGLNSKQVSLSAVHQMMHGLSDGFWISGWALLPVVLLIVLAILQVPAIPSLGLGSIFAALLGWIHQPSTSITTITKTIMDGYVAHSGDKTIDTLLSKGGISSMLTSLALIVFALSLGGLLIKLNIIGSIINHLSGIVKTAGRVTLATAITCIGVNLMIGEHYLAIILPGQSFIHASDNLGIPRELFTRILNDAGAAVNAIVPWSVSGVFIAGALQINPLQFIPWAFFPFIVTILCLITGMFVKPSSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL08</accession>
    <name>H4GL08_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ATP-dependent Clp protease ATP-binding subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_9731</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9731"/>
    <dbReference type="EnsemblBacteria" id="EHS84774">
        <property type="protein sequence ID" value="EHS84774"/>
        <property type="gene ID" value="PS3_9731"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1756"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UVR" evidence="5">
        <location>
            <begin position="308"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="304"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS84774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="680" mass="74775" checksum="4C054CCB07E8A893" modified="2012-04-18" version="1">MNGYNTENRRYLINGREVTPEEFAQYRQTGQLPGNPTPTKQAQAGQIKSDGLLSKLGRNLTQEARDGKLDPVIGRNQEIQETAEILARRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDVSSLEAGTQYRGSFEENMQKLVDEVKQAGNVILFFDEIHQIIGAGNAGDASGSKGMADILKPALSRGEITIIGATTQDEYRNTIMKDAALSRRFNQVTVNAPSKEDTLKILQGIRDLYEKHHNVELPDEVLKAAIDYSVQYMPQRQLPDKAIDLIDMTATHLAAQHPVKDANAIEAEIKQLEDQQQKAADKEDYQAAQEAKEKIESLKQQLDDHSTAERAVATPNDVAAAVERMTGIPVSKMGASDIERLKGLNDRLAGKVIGQDEAVNAVARAIRRNRAGFDEGNRPIGSFLFVGPTGVGKTELAKQLALDMFGSTDDIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSNTLTEKVRRHPYSIVLFDEIEKADPQVITLLLQVLDDGRLTDGQGNTIDFKNTIVIATSNAGFSNDAQLTEDQPTLIKRLTPYFRPEFLNRFNAIVEFKALSKDDLKAIVNLMLDGVNQTLAKKGMDVEVTDAAKDLMIEEGYDETMGARPLRRVIEQQIRDKVTDFYLDHLEAKHLLADVSDGTIVISERSDSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL15</accession>
    <name>H4GL15_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative D-lactate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_3966</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122747.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3966"/>
    <dbReference type="EnsemblBacteria" id="EHS84781">
        <property type="protein sequence ID" value="EHS84781"/>
        <property type="gene ID" value="PS3_3966"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1765"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="3">
        <location>
            <begin position="5"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="4">
        <location>
            <begin position="110"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS84781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="329" mass="35934" checksum="4900DD53A7740E1E" modified="2012-04-18" version="1">MTTILMTSVRDDEMDAINQYAEQHNITIKTTPKLIEDALDLTTDIDGLVIQQRDPVPDHVYPILKEHGLKQLASRTAGYDMVNVPLAHENGLIVTNVPAYSPRSVAEFALMQIFRLLRNTYAFDHRVAQNDFRWAGLQSIEIHSATIGIIGVGRIGGTLAKLLKALDARVLGYDVNPRSDLAGIVEYVSKDELLQQSDVVSLHVDLNPTSVNLLTASDFQLMKNSAGLVNASRGPVVNTSDLIAALEAHTIKAVALDTVEGEGPVFNADLRQKVISDPQIAKLHALENVILTPHVAFFTNIAVQNMVDFALDDVLTILNGGHSSHEIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL16</accession>
    <name>H4GL16_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA large subunit methyltransferase H</fullName>
            <ecNumber evidence="1">2.1.1.177</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">23S rRNA (pseudouridine1915-N3)-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">23S rRNA m3Psi1915 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">rRNA (pseudouridine-N3-)-methyltransferase RlmH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rlmH</name>
        <name evidence="2" type="ORF">PS3_13414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>pseudouridine(1915) in 23S rRNA + S-adenosyl-L-methionine = H(+) + N(3)-methylpseudouridine(1915) in 23S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10221"/>
            <dbReference type="Rhea" id="RHEA-COMP:10222"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74486"/>
            <dbReference type="EC" id="2.1.1.177"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA methyltransferase RlmH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.177" evidence="1"/>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122748.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13414"/>
    <dbReference type="EnsemblBacteria" id="EHS84782">
        <property type="protein sequence ID" value="EHS84782"/>
        <property type="gene ID" value="PS3_13414"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1766"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070038">
        <property type="term" value="F:rRNA (pseudouridine-N3-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18081">
        <property type="entry name" value="RlmH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00658">
        <property type="entry name" value="23SrRNA_methyltr_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003742">
        <property type="entry name" value="RlmH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33603">
        <property type="entry name" value="PTHR33603"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02590">
        <property type="entry name" value="SPOUT_MTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004505">
        <property type="entry name" value="MT_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00246">
        <property type="entry name" value="tRNA_RlmH_YbeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="159" mass="18118" checksum="F92372A5843632D5" modified="2012-04-18" version="1">MTIKIIGVGKLKEKYFKAGIAEYAKRFNRYTKFEIIEVPDEKAPESLSSAEMQSVMEKEGARILDKIKDREYVFALAIQGQERSSEEFAKEINQLTTYGHSDLTFVIGGSLGLSQAVMQRANTTISFGRFTLPHQLMRLVLTEQVYRAYTIINGLPYHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL17</accession>
    <name>H4GL17_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_13012</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S1C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122749.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13012"/>
    <dbReference type="MEROPS" id="S01.B81"/>
    <dbReference type="EnsemblBacteria" id="EHS84783">
        <property type="protein sequence ID" value="EHS84783"/>
        <property type="gene ID" value="PS3_13012"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1767"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="304"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="413" mass="42383" checksum="D3483C7DD595AF73" modified="2012-04-18" version="1">MRNSNHGRNRQLFWMKVATVGIVAGLIGGGIFWGVQKTISSVWPTIVSTVASGFGSSGGAKVHNSKVATSSSTKAYNKVKGAVVSVINQQSTSTSNMGDFGYSSSSSSANSSGLETAAEGSGVIYKKSAGTAYIVTNNHVVSGASAVSVILASGRKVSAKIVGTDETTDLAVLKISASHVKTVAEFGNSKQVQAGQDVLAIGSPMGTEYANSVTKGIVSAVNRTIRASDASVATKVIQTDTAINSGNSGGPLINMAGQVIGINSMKLSSSIDGTSVEGMGFAIPSNEVVSVINQLVKNGSISRPSLGISMLDLDSVTESDQTEVLKLPTNVTSGVVVMSVTDDGAAAKAGLSQYDVITKFGKTTVTDASDLRTALYKYKVGDRVKITYYHDGQEKTATVTLSEASNSSSSTNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL22</accession>
    <name>H4GL22_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_15025</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122754.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15025"/>
    <dbReference type="EnsemblBacteria" id="EHS84788">
        <property type="protein sequence ID" value="EHS84788"/>
        <property type="gene ID" value="PS3_15025"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1772"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="4"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="7">
        <location>
            <begin position="132"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="132"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="235" mass="26800" checksum="04016286789E0775" modified="2012-04-18" version="1">MSKKILVVDDEKPISDIIKFNLEKEGYEVVVAFDGEEALEKVESEKPDLLVLDLMLPKVDGLEVAKQVRAKYTTPIIMATAKDSELDKVLGLELGADDYVTKPFSNRELVARVKANLRRQEVVTSAVEDGREADIQIGDLTIHPDAYTVTKRGENINLTHREFELLHYLAQHQGQVINRENLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPQWLITRRGVGYFLANPNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL41</accession>
    <name>H4GL41_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7 8">Elongation factor Tu</fullName>
            <shortName evidence="7">EF-Tu</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tuf</name>
        <name evidence="10" type="ORF">PS3_14278</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122773.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14278"/>
    <dbReference type="EnsemblBacteria" id="EHS84671">
        <property type="protein sequence ID" value="EHS84671"/>
        <property type="gene ID" value="PS3_14278"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1796"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01884">
        <property type="entry name" value="EF_Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03697">
        <property type="entry name" value="EFTU_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_B">
        <property type="entry name" value="EF_Tu_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041709">
        <property type="entry name" value="EF-Tu_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033720">
        <property type="entry name" value="EFTU_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004541">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_bac/org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00485">
        <property type="entry name" value="EF-Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="11"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="20"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="82"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="137"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS84671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="396" mass="43491" checksum="79BC41657641913B" modified="2012-04-18" version="1">MAEKEHYERTKPHVNIGTIGHVDHGKTTLTAAITKVLAAKGLAQAEDYADIDAAPEEKERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLARQVGVEYIVVFLNKTDLVDDDELVDLVEMEVRDLLSEYDFPGDDIPFVRGSALKALEGDPEQEKVIEHLLDVVDEYIPTPDRPTDLPFMMPVEDVFTITGRGTVASGRIDRGTVKVGDEVEIVGLKEDVLKTTVTGVEMFHKTLDLGEAGDNVGVLLRGVAHDQIERGQVLAQPGSIQTHKQFKGEVYVMTKEEGGRHTPFFSNYRPQFYFHTTDVTGTIELPDGVEMVMPGDNVTFTVELQKPVALEKGLKFTIREGGHTVGAGVVSEVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL74</accession>
    <name>H4GL74_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cell division protein DivIB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">divIB</name>
        <name evidence="9" type="ORF">PS3_12212</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Cell division protein that may be involved in stabilizing or promoting the assembly of the division complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <text evidence="7">Localizes to the division septum.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the FtsQ/DivIB family. DivIB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122806.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12212"/>
    <dbReference type="EnsemblBacteria" id="EHS84704">
        <property type="protein sequence ID" value="EHS84704"/>
        <property type="gene ID" value="PS3_12212"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1837"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00912">
        <property type="entry name" value="DivIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026580">
        <property type="entry name" value="DivIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034746">
        <property type="entry name" value="POTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013685">
        <property type="entry name" value="POTRA_FtsQ_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08478">
        <property type="entry name" value="POTRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51779">
        <property type="entry name" value="POTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 7 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="1 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="POTRA" evidence="8">
        <location>
            <begin position="80"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS84704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="281" mass="31640" checksum="DDDF1D95EB68E244" modified="2012-04-18" version="1">MPTPSGRQEHERYQNILKNLEQRNRLANEPDVQKRGAGLHHSIAHFHRSMKRGNRERMLSIVIPFSLILAFMLYIVSPFSKVNKIQVTGCDNLSAQQVKQAANLKSGNFIWRVFYQQAAVEKVARKNNLQINQLKIELTGPQSIRVVVKEYPIVGIVTNGSRSHYLLASGKKTAVSGDVTSFIQYQGFTKHAKQLTATTAQVGRISTAIRDGISEVVYSPSSDDPERLILYMNDGNTVYARISNLSTKMKYYPSIVKKMTDAGIVDLQYSAYSYSYNSKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL84</accession>
    <name>H4GL84_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">6-phosphogluconolactonase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_4364</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cycloisomerase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4364"/>
    <dbReference type="EnsemblBacteria" id="EHS84714">
        <property type="protein sequence ID" value="EHS84714"/>
        <property type="gene ID" value="PS3_4364"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1850"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011048">
        <property type="entry name" value="Haem_d1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019405">
        <property type="entry name" value="Lactonase_7-beta_prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10282">
        <property type="entry name" value="Lactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51004">
        <property type="entry name" value="SSF51004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="349" mass="38296" checksum="08326A816CFF8046" modified="2012-04-18" version="1">MIILKEAIIVTEKFLIGTYTKKTSQGIYGVTLDSEQASLSPVWLVAKSGKPAYLQVLNDHLYSVKQIGDEGGVASYVLQGDQAELLKTNLSVVAPPAYVAVDQARSLLYSANYHAGTVTVYHINDDQSLTQTDEVKHSGVTGPRPEQDNPHAHYADLTPDHRLVVCDLGLDQTIVYDVSENGQLTFVSTYQHEAGFGTRHLTFSPDGKVAYVLGELSSQLEVLKYDANTGSFEHLQTIKTIPDDWTAHNGAAAIHISSDGKYVYTSNRGENTLAVFAVQDDSTVKHIQSISTEGDFPRDFDLSKNEDFLVASNQESDNLTLYARDPETGLLTLLQKDVACPEPVCVKQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL94</accession>
    <name>H4GL94_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage antirepressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_18894</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000079">
        <property type="protein sequence ID" value="EHS84623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122826.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000079.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS84623">
        <property type="protein sequence ID" value="EHS84623"/>
        <property type="gene ID" value="PS3_18894"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1863"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014054">
        <property type="entry name" value="Phage_regulatory_Rha"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09669">
        <property type="entry name" value="Phage_pRha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02681">
        <property type="entry name" value="phage_pRha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS84623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="256" mass="30031" checksum="FBA813F4619DA670" modified="2012-04-18" version="1">MENQVEIIKQVNDQDVMDSRDVAKMIGKIHRHLMRDIRRYINDLEESPKLDSRKFFIESSYESQQGKTLPCYLLTKQGCEFVANKFTGKKGTIFTATYVGLFNQYQEEHNGKLIGSKDKKLTKDNLEFKREWLLEMKRQNDNKEHQLRNEDAKIWLELADRADEYGETRMALDMRNDVIGMMTRLPISGREEYSASQIGEKLGVSPLQIGKWGNILGIKSDHHLSYKTAEGAWRYYPKAVELFQKNQFKIQEDFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL97</accession>
    <name>H4GL97_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Holliday junction ATP-dependent DNA helicase RuvB</fullName>
            <ecNumber evidence="9">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ruvB</name>
        <name evidence="11" type="ORF">PS3_17496</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a complex with RuvA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the RuvB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17496"/>
    <dbReference type="EnsemblBacteria" id="EHS84596">
        <property type="protein sequence ID" value="EHS84596"/>
        <property type="gene ID" value="PS3_17496"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1873"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00016">
        <property type="entry name" value="DNA_helic_RuvB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041445">
        <property type="entry name" value="AAA_lid_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004605">
        <property type="entry name" value="DNA_helicase_Holl-junc_RuvB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008824">
        <property type="entry name" value="RuvB-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008823">
        <property type="entry name" value="RuvB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42848">
        <property type="entry name" value="PTHR42848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17864">
        <property type="entry name" value="AAA_lid_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05491">
        <property type="entry name" value="RuvB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05496">
        <property type="entry name" value="RuvB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00635">
        <property type="entry name" value="ruvB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 9 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="AAA" evidence="10">
        <location>
            <begin position="56"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="64"/>
            <end position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS84596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="337" mass="37317" checksum="F5795E860AC3FF66" modified="2012-04-18" version="1">MDENLRDLTSNEVNDEQEAAVELSLRPQRLAEYIGQAALKEKLTVYIQAAKAREEALDHVLLYGPPGLGKTTLAMVIANEMAVQIRTTSGPAIEKPGDLVALLNELQPGDVLFIDEIHRLPKVVEEMLYSAMEDYYIDIVVGEGPTAHPVHFPLPPFTLIGATTRAGALSAPLRDRFGIVERMNYYQAAELAQIIKRSATIFETAIDETGAHELALRSRGTPRIANRLLKRVRDFSEVAGQARIDLDTVKKSLDLLKVDNLGLDEVDRKLLTTMINYYHGGPVGLKTIAANIGEETTTIEEMYEPYLLQIGLLSRTPRGRQVTMAAYDHLKIPYPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLB9</accession>
    <name>H4GLB9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_11878</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. RarA/MGS1/WRNIP1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122851.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11878"/>
    <dbReference type="EnsemblBacteria" id="EHS84618">
        <property type="protein sequence ID" value="EHS84618"/>
        <property type="gene ID" value="PS3_11878"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1897"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032423">
        <property type="entry name" value="AAA_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021886">
        <property type="entry name" value="MgsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16193">
        <property type="entry name" value="AAA_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12002">
        <property type="entry name" value="MgsA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="SSF48019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="37"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS84618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="424" mass="46432" checksum="73D815000D7B82AC" modified="2012-04-18" version="1">MEPLAYRMRPQSLAEIVGQQHLVGQDKIIWRMVKAKMLSSMILYGPPGTGKTSIASAIAGSTKYAFRTLNAATDSKKDLQIVAEEAKMSGTVILLLDEIHRLDKSKQDFLLPHLESGRIILIGATTENPYISINPAIRSRTQIFEVKPLTIDEIGIAIDRAITDPQRGLGQMNLSLDDDARRQLISSTNGDLRSALNGLELAAKSTAPGSDGVIHLTLPVIEESIQKKSISLDKNGDGHYDVISAFQKSIRGSDTDAALHYLGRLIEGGDLPIIARRLMVIAYEDIGLANPPAAARVVTAVQAAEKLGFPEARIPLANAVIELCLSPKSNSAISAIDKAIARIRQGNTGDVPDYLKDAHYQGAEKLGHGIDYLYPHDYPEDWVAQQYLPEPIKDDQYYEPKTNGNYEKAYADQYHRLLKAQGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHQ2</accession>
    <name>H4GHQ2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uracil-xanthine transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_3358</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000001">
        <property type="protein sequence ID" value="EHS87562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121586.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000001.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3358"/>
    <dbReference type="EnsemblBacteria" id="EHS87562">
        <property type="protein sequence ID" value="EHS87562"/>
        <property type="gene ID" value="PS3_3358"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="387"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="443" mass="46170" checksum="5C26B27D9554EC18" modified="2012-04-18" version="1">MADQIEKSLVVGPDDRVPTGEATLLGLQHVLAMDVYVPPIILAGSLAMGAANSTGLLQSTFLAAGIGTILQTAIFMKMPVSQGPSFVPLGAAAGVVLASGGLNHGGMATLLGALTVGAIVLILLGLSGVFQKIINTLVPAVVGGTIITCVGLSLIPSALNDNVFEASGNIYQNMILASITALTLLICVAISIRFPRVQKLFKTGSIVISLLVGTLVSAMMGRFDWKTVADAAWIGLPQRTMFHWGINFDLTAILTFIIIYAILTTETTGTWFAMGAVTNHQITDRQWNRGIIGEGLSCLVAAIAGATPVTGYSTNAGIVSITGVASKRVFISAGIWFIILGFFTKLSAFLSAIPAPVIGGVFAIITVTIMLNGLNVIRRTKTGESDLYIIGIPIVLTLAIILLPTKVMNASPQMVQYLLGSPIAIAAITAVVLNLIMPSRSQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHQ5</accession>
    <name>H4GHQ5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">2,3-bisphosphoglycerate-dependent phosphoglycerate mutase</fullName>
            <shortName evidence="4">BPG-dependent PGAM</shortName>
            <shortName evidence="4">PGAM</shortName>
            <shortName evidence="4">Phosphoglyceromutase</shortName>
            <shortName evidence="4">dPGM</shortName>
            <ecNumber evidence="4 5">5.4.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gpmA</name>
        <name evidence="6" type="ORF">PS3_10208</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.11" evidence="4 5"/>
    <dbReference type="EMBL" id="AICN01000002">
        <property type="protein sequence ID" value="EHS87560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121589.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000002.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10208"/>
    <dbReference type="EnsemblBacteria" id="EHS87560">
        <property type="protein sequence ID" value="EHS87560"/>
        <property type="gene ID" value="PS3_10208"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.13"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046538">
        <property type="term" value="F:2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01039">
        <property type="entry name" value="PGAM_GpmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005952">
        <property type="entry name" value="Phosphogly_mut1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11931">
        <property type="entry name" value="PTHR11931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01258">
        <property type="entry name" value="pgm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="2 4" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="21"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="87"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="114"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="183"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="4">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="4">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="231" mass="26270" checksum="DCFF75135E30F721" modified="2012-04-18" version="1">MAKLVLIRHGQSEWNLANLFTGWEDVDLSEKGVAQAKAAGKAIKEHGLEFDYAYTSVLKRAIKTLHYALEESDQMWIPEFKTWRLNERHYGALQGKNKADAAEKYGDEQVHIWRRSYDVLPPLLSADDEGSAAKDRRYANLDPRTIPGGENLKVTLERVIPLWEDEIAPKLLDNKNVIIAAHGNSLRALSKYIENISDEDIMNLEMATGEPVVYDFDEKLNVLSKDKYPVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHQ9</accession>
    <name>H4GHQ9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetate kinase</fullName>
            <ecNumber evidence="6">2.7.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Acetokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ackA</name>
        <name evidence="8" type="ORF">PS3_11883</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>acetate + ATP = acetyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:11352"/>
            <dbReference type="ChEBI" id="CHEBI:22191"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Mg(2+). Can also accept Mn(2+).</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the acetokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.1" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000003">
        <property type="protein sequence ID" value="EHS87554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121593.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000003.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11883"/>
    <dbReference type="EnsemblBacteria" id="EHS87554">
        <property type="protein sequence ID" value="EHS87554"/>
        <property type="gene ID" value="PS3_11883"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.21"/>
    <dbReference type="UniPathway" id="UPA00340">
        <property type="reaction ID" value="UER00458"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008776">
        <property type="term" value="F:acetate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00020">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004372">
        <property type="entry name" value="Ac/propionate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000890">
        <property type="entry name" value="Aliphatic_acid_kin_short-chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023865">
        <property type="entry name" value="Aliphatic_acid_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21060">
        <property type="entry name" value="PTHR21060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00871">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000722">
        <property type="entry name" value="Acetate_prop_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00471">
        <property type="entry name" value="ACETATEKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00016">
        <property type="entry name" value="ackA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01075">
        <property type="entry name" value="ACETATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01076">
        <property type="entry name" value="ACETATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="6">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="207"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="282"/>
            <end position="284"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="330"/>
            <end position="334"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="399" mass="43558" checksum="7AAEA39DFA26DBC6" modified="2012-04-18" version="1">MSKTIAINAGSSTVKFKLFDMPSEEVIAEGNLERIGLEMGHAKIKYEEDQVHEEDTPFADHEAAVEYLLHQLKNLGIVADYNEIDAVGHRIVAGGEFFKDSAVIDEDVMDKIDQLAEYAPLHDPAELQGIKAFKDVLPDAFAVAVFDTSFHADMPKMNALYSTPYEWYEKYGARKYGAHGTSHRYVAQRAADLLGRPLEDLKLITCHIGAGASITAIKNGKSYDTSMGFSPLAGVTMATRSGDVDPSMVNFVANKLGKNGDEMIDILNHQSGLLGISGVSSDMRDVEAAAKEGNERAELALEINVNRVVKYIGSYVAELGGVDAIVFTAGVGENDPNFRKQIADQLSFLGTGIDDEKNNVRGVERDISSDDATVKCLLIPTDEELMIVHDIERLRKAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHR3</accession>
    <name>H4GHR3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Amino acid transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_20866</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000004">
        <property type="protein sequence ID" value="EHS87543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121597.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000004.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20866"/>
    <dbReference type="EnsemblBacteria" id="EHS87543">
        <property type="protein sequence ID" value="EHS87543"/>
        <property type="gene ID" value="PS3_20866"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amoino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="AA_permease" evidence="7">
        <location>
            <begin position="18"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="328" mass="35988" checksum="2E0ADD04D5E6AE97" modified="2012-04-18" version="1" fragment="single">MSANEKIEHLERKMETRHIQMISLGGVIGTGLFLSSGYTIQQAGPIGTIIAYLLGAIIVYAVMLCLGELSVAMPYTGSFHVYAKDLISPATGFTVAILYWLTWTIALGSEFTAAGIIMRQWFPHVPVWIWSLLFIILIFLSNVFSVRIFAESEFWFAAIKVIAILVFIVLGALAIVGIIPMKGYQHAPLLTNLTKDGWFPTGFGGVFTTMLTVNFAFSGTELIGITAGEAKDPEKTLPKAIHTTLWRLIIFFIGSITVMACLIPYRQAGVSESPFVHVFKLMGIPYAADIMNFVVLTAIISAANSGLYASTRMLWSLSNEGTIPRRFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHU4</accession>
    <name>H4GHU4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH_40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_21909</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21909"/>
    <dbReference type="EnsemblBacteria" id="EHS87500">
        <property type="protein sequence ID" value="EHS87500"/>
        <property type="gene ID" value="PS3_21909"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.64"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029491">
        <property type="entry name" value="Helicase_HTH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14493">
        <property type="entry name" value="HTH_40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_40" evidence="1">
        <location>
            <begin position="253"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="355" mass="41223" checksum="84963A6D8A65F901" modified="2012-04-18" version="1">MVSGEVLMNPYILTLFNRQPRRIRVIENILKNRRSEANLFWGYNYQILGALGAERQLKRQDYDQQLAQWVKDGLLKIDDQQASLTEAGLEQVKTFWDQHYQPHFIQWAWVTNYQIFANRVLLALQVISQYQHQDHQYLPLSLSEYEMNRVRQWLRALNPSDVKLITNCLQEVTEELASVDERLAILLTYRLIGYQTSGWTNQQAAQHLAVTIDEIPVMWRDLWLRMAATIASQSGPLTSLTQDLISLSPASQSAMKTIQTFQSGISIDEVSQRRHLKISTVREHILEIAILRPELISISALIPTSNLAKLKATYQVSAFNWQFQEDSDEASAFFEFRLYQIMRCHRENGDYPSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHU9</accession>
    <name>H4GHU9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Pyruvate kinase</fullName>
            <ecNumber evidence="4 14">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PS3_4912</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the pyruvate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="4 14"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121633.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4912"/>
    <dbReference type="EnsemblBacteria" id="EHS87505">
        <property type="protein sequence ID" value="EHS87505"/>
        <property type="gene ID" value="PS3_4912"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.73"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PTHR11817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="SSF50800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="SSF52935"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 14 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0630">Potassium</keyword>
    <keyword evidence="13 17" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PK" evidence="15">
        <location>
            <begin position="1"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="PK_C" evidence="16">
        <location>
            <begin position="357"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EHS87505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="473" mass="51804" checksum="EBC090439E279EFA" modified="2012-04-18" version="1">MKKTKIVSTLGPASTDVETITKLIESGANIFRFNFSHGDHEEHLGRMNNVHEAEKRTGKKVAIMLDTKGAEIRTTVQKEGKIAFETGDKVRISMDDSIEGTHDKIAVTYPGLYDDVKEGGHVLFDDGLLDMLVEEKDEANKELVCRVLNAGVLGSRKGVNAPGVSINLPGITEKDTSDILFGLDNGISYIAASFVRKPQDIEDIRVLLKQKNMEDVLVLPKIESQEGIDNFEAIMEVSDGLMVPRGDMGVEIPAENVPLIQKMMIRRCNELGKPVITATQMLDSMQENPRPTRAEVSDVANSVFDGTDATMLSGESANGDYPVESVATMNRIDIKAENNLLQYGTEEFNFDTSDVTETIGSAVSRAARDMGIKTIVATTTSGYTARMISKYRPNADIVALTYDERTQMSLMLNWGVQPYVIDHPETSDEMFEMASQKAKELGFAQAGDQIIVVSGLLHGGAGTTNLMRIQTVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHX6</accession>
    <name>H4GHX6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphate starvation-inducible protein PhoH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_4359</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121660.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4359"/>
    <dbReference type="EnsemblBacteria" id="EHS87532">
        <property type="protein sequence ID" value="EHS87532"/>
        <property type="gene ID" value="PS3_4359"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.102"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003714">
        <property type="entry name" value="PhoH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02562">
        <property type="entry name" value="PhoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoH" evidence="3">
        <location>
            <begin position="114"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="326" mass="35672" checksum="8C5BD76D26E5CED6" modified="2012-04-18" version="1">MTATETISVQFKLSKPEEQVGLLGIQDQFVSLFEEGMGVEMSPFGDQLTITGEAEAVHQTEAVLGELVKLLDQQIKVSSTDIVSAMKMAKKGTLEYFGDLYRETIVKDRKGNPIRVKTFGQRQYVHAIKQRDVTFGIGPAGTGKTYLAVAMAVAALKRGEVERIILTRPAVEAGESLGFLPGDLKEKVDPYLRPIYDALNSILGADHTERLMDRGVIEIAPLAYMRGRTLDGAFVILDEAQNTTNAQMKMFLTRLGFGSKMVVNGDISQIDLPRGAKSGLVNAQQILRGIDAIEFVHFTAGDVVRHPVVAKIINAYEAEQSTPKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHY5</accession>
    <name>H4GHY5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal protein L11 methyltransferase</fullName>
            <shortName evidence="2">L11 Mtase</shortName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">prmA</name>
        <name evidence="3" type="ORF">PS3_13397</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Methylates ribosomal protein L11.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-lysyl-[protein] + 3 S-adenosyl-L-methionine = 3 H(+) + N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:54192"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13826"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the methyltransferase superfamily. PrmA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121669.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13397"/>
    <dbReference type="EnsemblBacteria" id="EHS87541">
        <property type="protein sequence ID" value="EHS87541"/>
        <property type="gene ID" value="PS3_13397"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.113"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00735">
        <property type="entry name" value="Methyltr_PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004498">
        <property type="entry name" value="Ribosomal_PrmA_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000401">
        <property type="entry name" value="RPL11_MTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00406">
        <property type="entry name" value="prmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="319" mass="35109" checksum="922B8FC90A30E103" modified="2012-04-18" version="1">MQWTAIVVETTSEVVEAVSYLLTEAGANGIQVEDAADYAQLKPGKYGPYGEIVDPESLKHRQSGAAITGYFPPNQFGPELIDEIKTRVAKLNEFGLNPGSFKVTFAPVDETDWATEWQKYYHPVRVTHELTIVPQWETYQAHDSEKIIFMDPGMAFGTGTHPTTQLMLQALEISLRGGERMIDVGTGSGILSIAAKLLGAGDVRAYDIDQIAVDSARRNIELNPQAQGITFGVNSLLDGIHTQVDLIVANILAEIIVPLVPQAFENLTPGGKFLTSGIISDKFELCRDTMIKQGFKIDQTLRMKDWYGIIAHKPAEDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHZ0</accession>
    <name>H4GHZ0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoenolpyruvate-dependent sugar phosphotransferase system EIIB.1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_9708</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000006">
        <property type="protein sequence ID" value="EHS87469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121674.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000006.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9708"/>
    <dbReference type="EnsemblBacteria" id="EHS87469">
        <property type="protein sequence ID" value="EHS87469"/>
        <property type="gene ID" value="PS3_9708"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.119"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036095">
        <property type="entry name" value="PTS_EIIB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013011">
        <property type="entry name" value="PTS_EIIB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003501">
        <property type="entry name" value="PTS_EIIB_2/3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02302">
        <property type="entry name" value="PTS_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52794">
        <property type="entry name" value="SSF52794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51099">
        <property type="entry name" value="PTS_EIIB_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PTS EIIB type-2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="94" mass="10156" checksum="49DFE9DD46CCA487" modified="2012-04-18" version="1">MKTLMVTCGSGIATSTVAEGKIKDYLAQAGVLDQVKIYKGNIAEYVNKVDDYDVFVSTTVVPDEIKDRVISGLPLLTGIGADKVYQQIMDKLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI09</accession>
    <name>H4GI09_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Holo-[acyl-carrier-protein] synthase</fullName>
            <shortName evidence="9">Holo-ACP synthase</shortName>
            <ecNumber evidence="9">2.7.8.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">4'-phosphopantetheinyl transferase AcpS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">acpS</name>
        <name evidence="11" type="ORF">PS3_6719</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>apo-[ACP] + CoA = adenosine 3',5'-bisphosphate + H(+) + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="EC" id="2.7.8.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the P-Pant transferase superfamily. AcpS family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the P-Pant transferase superfamily. Gsp/Sfp/HetI/AcpT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.7" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000007">
        <property type="protein sequence ID" value="EHS87460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121693.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000007.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6719"/>
    <dbReference type="EnsemblBacteria" id="EHS87460">
        <property type="protein sequence ID" value="EHS87460"/>
        <property type="gene ID" value="PS3_6719"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.143"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00101">
        <property type="entry name" value="AcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002582">
        <property type="entry name" value="ACPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004568">
        <property type="entry name" value="Ppantetheine-prot_Trfase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="SSF56214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00516">
        <property type="entry name" value="acpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00556">
        <property type="entry name" value="pantethn_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACPS" evidence="10">
        <location>
            <begin position="4"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="123" mass="13574" checksum="3CAFEAAC0EF1187C" modified="2012-04-18" version="1">MIKGIGIDITEIKRVQAAVNKHAQFIPKVLTPAEQAQLESLRGQRYWEYISSRFSLKESFSKAMGTGIGTQVGFQDVSFINNDLGRPEVFQSIFSGRAHASVSHTDQLVMTEVILEEGSDGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI15</accession>
    <name>H4GI15_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_21056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000008">
        <property type="protein sequence ID" value="EHS87448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87448">
        <property type="protein sequence ID" value="EHS87448"/>
        <property type="gene ID" value="PS3_21056"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.152"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018337">
        <property type="entry name" value="Cell_wall/Cho-bd_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01473">
        <property type="entry name" value="Choline_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19127">
        <property type="entry name" value="Choline_bind_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="199" mass="23140" checksum="37B2A37DAD71234D" modified="2012-04-18" version="1">MTDQVTDIDGATYWFNADGVMQTNAFYSNDGKLYYFGEDGKEYKDQFYSNWGNTYYFGADGARYTDQWYSNWGNTYYFGDDGILVKSTVKTIDGTDYLFNSEGVSTKLSDVKDQFVTVDGKVYYFDAEGHEYKDQFYVNWGNTYYFGEDGARYTNQWYSNWGHVYYFGSDGALLKNTTRTINGTEYYFDNDGVAYNVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI49</accession>
    <name>H4GI49_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 11 12">Uridine kinase</fullName>
            <ecNumber evidence="7 11 12">2.7.1.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cytidine monophosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Uridine monophosphokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">udk</name>
        <name evidence="14" type="ORF">PS3_17950</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP + cytidine = ADP + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 11 12">
            <text>ATP + uridine = ADP + H(+) + UMP</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5 11 12">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 11 12">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 11 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 11 12">Belongs to the uridine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="7 11 12"/>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121733.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17950"/>
    <dbReference type="EnsemblBacteria" id="EHS87388">
        <property type="protein sequence ID" value="EHS87388"/>
        <property type="gene ID" value="PS3_17950"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.194"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00579">
        <property type="reaction ID" value="UER00640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044211">
        <property type="term" value="P:CTP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00551">
        <property type="entry name" value="Uridine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026008">
        <property type="entry name" value="Uridine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00235">
        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 12 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PRK" evidence="13">
        <location>
            <begin position="8"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="13"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00021478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS87388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="213" mass="24697" checksum="339FE6BC9291F61E" modified="2012-04-18" version="1">MARKRPVVIGVTGGSGSGKTTVSHAIYDLMNGQSMEIITQDTYYNDQASMTMEERKAVNYDHPLAFDNDLLYQQLDQLRHNQAIEMPVYNYAEYTRSNETRHVEPPEIIILEGILILDDERLRNLMDIKVYVDTDDDIRIIRRIQRDMVERGRSIESIIDQYLTTVKPMYHEFIEPTKRYADIIVPEGGKNQVAIDLLATKVRDILVQRGHRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI64</accession>
    <name>H4GI64_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Bis(5'-nucleosyl)-tetraphosphatase (symmetrical)</fullName>
            <ecNumber evidence="2">3.6.1.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_15074</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + P(1),P(4)-bis(5'-adenosyl) tetraphosphate = 2 ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:24252"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58141"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.1.41"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.41" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121748.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15074"/>
    <dbReference type="EnsemblBacteria" id="EHS87403">
        <property type="protein sequence ID" value="EHS87403"/>
        <property type="gene ID" value="PS3_15074"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.217"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005249">
        <property type="entry name" value="YqeK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00488">
        <property type="entry name" value="TIGR00488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="4">
        <location>
            <begin position="34"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="210" mass="23859" checksum="3CE68EFE1A2B28A6" modified="2012-04-18" version="1">MTNNDQQFKYTGEFAYLDRDQLVTKIQAALSPERFKHVLRVETTARQLARQYGVDEQLASIAGLCHDYAKERPAEDFIQVIKAKQLEPDLLSAGNAIWHGVVGAEMVADELLVHDQRILDAIRQHTFGGPVMTKLSQVLYMADYIEPGRDFPGVDQARQITQQSLAKGVAFQTWHTLNYLVQKGASVYPATIDTYNAWVPQYRKEFLLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI95</accession>
    <name>H4GI95_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Alkaline phosphatase superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_13741</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LTA synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121779.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13741"/>
    <dbReference type="EnsemblBacteria" id="EHS87316">
        <property type="protein sequence ID" value="EHS87316"/>
        <property type="gene ID" value="PS3_13741"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.259"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008484">
        <property type="term" value="F:sulfuric ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012160">
        <property type="entry name" value="LtaS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005091">
        <property type="entry name" value="Mmb_sulf_HI1246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase" evidence="9">
        <location>
            <begin position="251"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="670"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="684"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="481"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR005091-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005091-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005091-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="710" mass="80364" checksum="35C9051C4AE05D52" modified="2012-04-18" version="1">MFKKMGNLLNTRLGLFLVLTVLFWLKTLLAYFVDFSLGETGLLQYILLLVNPLGTTILLFGLAFYFKKSRFFYPVLMEIDILNTLLLYLNVIYYREFTDFMTIATIAGYSKVNQGLSGSSLALTKPHDVLFWLDIAIILILMILRKIKFDPRAVTHRFAFAITSIALVLIGTNLMVAEMDRPQLLGRTFDRTYIVKYLGIDTFTAYNVIQNQQVNQMRSSATKSQLNQVRKFTESIYAKPNDKMYGIAKGRNVIVIHLESFQQFLIGLKVNGQEVTPFLNSLVNSNDTYSFDNFFHQVGQGKTSDAENLLETSTFGLPQGSLFATLGSDNTFEGAPAILNQRRGYSSAVFHGNVASFWNRNNVYKNLGYQYFFDASYYDTSGDKATGYGLKDKLLFQNSVKYLEQLQQPFYVKFITVTNHFPFELDSEDEDSDFTAPDTGDSTVDNYFVTAHYLDQSLEEFFNYLEKTGLDKKSIIMIYGDHYGISNSENKSLASVIGKDANNWTNFDNVQMQRVPLMFVIPGTTGGKVYHTYGGEVDVLPTLLHLLGISTKRYIFFGTDLFSSQHRQVVAFRDNDFITNKFTSISGKIYSNTTGKLATLTKSEKKQIREDTNFVNQELKLSDALNQKNLLRFYTPKGFTKVVASKFNYSNGLKKLKQLAKKLGNKSTSLFSENGDKSTVDDYSTDAPEASHSSTSSNRIKITNPDSTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI99</accession>
    <name>H4GI99_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methylated DNA-protein cysteine methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_20643</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121783.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20643"/>
    <dbReference type="EnsemblBacteria" id="EHS87320">
        <property type="protein sequence ID" value="EHS87320"/>
        <property type="gene ID" value="PS3_20643"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.264"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036631">
        <property type="entry name" value="MGMT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="SSF46767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53155">
        <property type="entry name" value="SSF53155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_binding_1" evidence="6">
        <location>
            <begin position="79"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="161" mass="18413" checksum="8A50E8D3074E85A3" modified="2012-04-18" version="1">MIRTNWQSPLGEITILSDHQFLFGLWFKQANHYGAHYQLDEIALGTTDVADLTINWLTQYFNHQIVDPRLIPVHPVGTPFQMVVWQQLREIPYGQAKTYRELAQTLHSSPRAIGNAVGRNPISIIIPCHRILNVHQQMTGYAGGIARKQFLLELEGIPFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIA9</accession>
    <name>H4GIA9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_9218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121793.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9218"/>
    <dbReference type="EnsemblBacteria" id="EHS87330">
        <property type="protein sequence ID" value="EHS87330"/>
        <property type="gene ID" value="PS3_9218"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.274"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="6"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="136" mass="16010" checksum="98F9F4914DC013DE" modified="2012-04-18" version="1">MFPERLRALRRGQKITLRELCDVLNANLGPDERPNTPSQIGNWERGIRTPNYIEVRKLAEFFDVSLDYLTGRAERDDHDLAKLFFSSKDLSFNHTTLTSEDRYEIFQLIDGYLKGRQNRSETETFYGTQEELNLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIB0</accession>
    <name>H4GIB0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_4087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121794.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4087"/>
    <dbReference type="EnsemblBacteria" id="EHS87331">
        <property type="protein sequence ID" value="EHS87331"/>
        <property type="gene ID" value="PS3_4087"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.275"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="285" mass="33125" checksum="0799F61A3390D07E" modified="2012-04-18" version="1">MNPKKLKQLKKQHRKANPQSRQSTYIQQLTQYGELFQDFPRIKYLINLILVNDRLMQDQLLPQPVVKMLLPDDIQDEIFTYLNQHYPSGDPTGDRLWEEYSEALPKLDRLLRNYRDYLEATYGMWSFTNTKFISALSKYLQGAPVLEIMAGNGYISKGLHDLNHQQTIITTDDQSWVKENETGKHPVTKVQNYSAIAAIKNFHDQVDYVIMAWAPDKGDADWQVLQLIRQAYPELKLLVIGEYQGATNSQQFWQMAQLSQPDAVQPLNQALQSFDLVDEQIYLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIC4</accession>
    <name>H4GIC4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">PTS system galactitol-specific IIC component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PS3_3335</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121808.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3335"/>
    <dbReference type="EnsemblBacteria" id="EHS87345">
        <property type="protein sequence ID" value="EHS87345"/>
        <property type="gene ID" value="PS3_3335"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.302"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015577">
        <property type="term" value="F:galactitol transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013853">
        <property type="entry name" value="GatC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013014">
        <property type="entry name" value="PTS_EIIC_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004703">
        <property type="entry name" value="PTS_sugar-sp_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37324">
        <property type="entry name" value="PTHR37324"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03611">
        <property type="entry name" value="EIIC-GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006304">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51104">
        <property type="entry name" value="PTS_EIIC_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="218"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="427"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIC type-2" evidence="10">
        <location>
            <begin position="6"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="484" mass="53285" checksum="7064CE99F882C2AC" modified="2012-04-18" version="1">MLEAVNNFFTAFGASVVVPIMIFIIALFLRVKPKTAMMSAFYAGVGLTGFSWIISEFTPVVTKIIRQMVNTTGLNLPIVDIGWQAGSLASFGSTVGLSFFVFGLIFELIIFALGITKIFMASNLWNNFGFMIWGTMAYYVTHNFWLSLGLSFFMLLYALLLAEVQADRWSDYYGVKNASVASLHNIEQTVPAIILDPLWNLLGFNKVKMTPEYFKNKLGVFGEPTTLGALLGLIIGILGNLNSLGTVKAWGQILQFAIQLSAVMTIFPLVTNVFSKAFTPLAEEIDKNRKKESSEEGKKIDKINDKKRWFLGVDDGVGYGEPATIISGVILIPIMVLIAFVLPGNKTLPVVDLISIPFMVESIVAITRGNILKVIANGIVWFSLGLYASSWLGSVYTGAVSHYGAAIPAGVVLITSFNLMSRPLNALVFAAFITENPVWIGICIVVYLVLLFALRRYRPQIWTYLNKMADKNAGFAGSKDKIEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIC7</accession>
    <name>H4GIC7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phosphoketolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_5711</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XFP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121811.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5711"/>
    <dbReference type="EnsemblBacteria" id="EHS87348">
        <property type="protein sequence ID" value="EHS87348"/>
        <property type="gene ID" value="PS3_5711"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.308"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005593">
        <property type="entry name" value="Xul5P/Fru6P_PKetolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018969">
        <property type="entry name" value="Xul5P/Fru6P_PKetolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019790">
        <property type="entry name" value="Xul5P/Fru6P_PKetolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018970">
        <property type="entry name" value="Xul5P/Fru6P_PKetolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31273">
        <property type="entry name" value="PTHR31273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03894">
        <property type="entry name" value="XFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09363">
        <property type="entry name" value="XFP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09364">
        <property type="entry name" value="XFP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017245">
        <property type="entry name" value="Phosphoketolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS60002">
        <property type="entry name" value="PHOSPHOKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XFP_N" evidence="6">
        <location>
            <begin position="7"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="XFP_C" evidence="5">
        <location>
            <begin position="597"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="249"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="801" mass="90169" checksum="170C4FACBD110604" modified="2012-04-18" version="1">MTVDYDSKQYLESVDAYWRATTYLSAGAMFLMHNPLLRRELKPSDVKPKPIGHWGTIGPQNFIYAHLNRAIKKYDLNMFYIEGSGHGGQVMQTNSYIDGSYTELYPEFTQDEAGMAKLFKHFSFPGGTASHAAPEVPGSLHEGGELGYALSHGVGAILDNPDLIAAVEIGDGEFETGPLATSWFSNKFINPIKDGAVLPILQVNGFKISNPTIPARMSDEELTKYFEGMGWKPHFVSAFKTSDDPFSGYKDIMETHEQMAKELDAAVEEIKAIQKNARENNDNSLPAWPVVVVRAPKGWSGPTTDLDGNPIENSFRAHQVPLPVSQDDMEHVDMLVDWLKSYKPEELFDEDGHPTELVLANATEGSQRMALNPATNGGADQKPLALPDINDFALKFDQPGLVVAQDMIEWAKYLAKMMELNPNNFRGFGPDESKSNRLYTVLDQDKRQWMENIHEPNDENLAPQGRMIDSQLSEHQAEGWLEGYTLTGRHGFFATYESFGRVVDSMLTQHMKWLRKAKEQYWRKSIPALNFVDTSTVFQQDHNGYTHQDPGLLTHLFEKERPELIKEYIPADTNQLLAVSDVAFNDQDVINLFVTSKQPRAQWYSLDEAKTIVNNGLGYVDWASTDQGAEPDVVFAAAGTEPNEEALAAIDILHKAFPELKIRFINVVQPLKLMSQSRNPEGLSDAEFDHYFTADKPVIFAWSGFQDMLQSIFFDRHNHNLSVHGYRENGDITSTFDVRVMSKLDRFDLAKDAISKVAGYEEKATAFVQEMDNMLAKHTAYVHAEGKDLPEVTNWKWTGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GID5</accession>
    <name>H4GID5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Major facilitator transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_12185</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121819.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87356">
        <property type="protein sequence ID" value="EHS87356"/>
        <property type="gene ID" value="PS3_12185"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.324"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="294"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="204"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="388" mass="42339" checksum="9458B2757D7F55EB" modified="2012-04-18" version="1">MKKRQYSRWLVLGIFLLAACMRSPFTSLPSVIDQIAASFHQPATNLGILTTIPLICFGLCSTIVPSISRRWGNEVTLGVALLIMVIGSFLRIFNFVTLIFGTILVGIAITFLNVLLPAVITEKIPQKIGLLTGMYNVSLAIFSAIGAYVITPVTQATNWQTAIIGFTLLIGVTFIVWLPNLRFNHRHLVPIQAGNQTRIWTNPRAWLLLMYFGLQSFAFYIIVAWLPSIAMAAGLSNATASLVASSFQLLSLPAAFLIPTITPRFKNRVPLVIMAATPLLIGLILLLWPLNSLAYFIFISFLLGTGTSATFGIVITLFGLKTHSPEETSRLSGMVQSVGYILAALGPVIAGNIKAATGNWQASLWMTIIIVVIMIAFGALSERHQYIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GID6</accession>
    <name>H4GID6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <shortName evidence="5">G6PD</shortName>
            <ecNumber evidence="5">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">zwf</name>
        <name evidence="9" type="ORF">PS3_6499</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121820.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6499"/>
    <dbReference type="EnsemblBacteria" id="EHS87357">
        <property type="protein sequence ID" value="EHS87357"/>
        <property type="gene ID" value="PS3_6499"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.325"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="PTHR23429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1 5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="2 5" id="KW-0521">NADP</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G6PD_N" evidence="7">
        <location>
            <begin position="11"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="G6PD_C" evidence="8">
        <location>
            <begin position="192"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="87"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="489" mass="55651" checksum="DD0B68E700DCBF27" modified="2012-04-18" version="1">MATEIKAVITLFGATGDLAHRKLYPSLFNLFRKGFLADNFALLGTSRSEWSDADFQAKVRESISKVDEDQAGQYDAFVKHFFYQPHDVTNPEHYDKLNERIAELEDKFATEGNRLFYMSMAPRFFGTIAANIKSQGLLSDNGFNRLVIEKPFGRDFDSAQELNKELTETFDEDQIFRIDHYLGKEMVQNISALRFGNTAIEALWNNQYIDNIQVTLSEDMGVEERAGYYDQSGALRDMVQNHIMQVVAQLAMEQPLTFTDQDIRAEKAKALRSLRKYTPSEAAANFVRGQYAAGDNNNEYRKEDGVDPKSITETYVAAKLTFDSYRWAGVPFYVRTGKLLAGKFTRVDVVFKKPLIDIFATPANSNDQKLNNNVLTIYIDPTPGFSLTLNAKKVGQGYTTEPIDLTFNQSAEQKALSPEPYERLFHDALEGNSTNFASWSEIADAWQFVDVIRQVWDHEEPNFPNYQPGSMGPKEADELLARDGRQWVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIG1</accession>
    <name>H4GIG1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sugar transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_4589</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GRP transporter (TC 2.A.7.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000016">
        <property type="protein sequence ID" value="EHS87304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121845.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000016.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4589"/>
    <dbReference type="EnsemblBacteria" id="EHS87304">
        <property type="protein sequence ID" value="EHS87304"/>
        <property type="gene ID" value="PS3_4589"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.358"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015144">
        <property type="term" value="F:carbohydrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010651">
        <property type="entry name" value="Sugar_transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16119">
        <property type="entry name" value="PTHR16119"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06800">
        <property type="entry name" value="Sugar_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="291" mass="31010" checksum="FB73EBCF6A8958D8" modified="2012-04-18" version="1">MNYLIGLIPALGWGIMPLLATKAGGSEKNQIFGIGAGASIIGILAFIITKPSVSWQAFIFSMVCGAIWSTAQIGQFISFKRMGVSTTVPLSTVFQLVGNSIIGTLWLGEWQGAKALTIGFGALVIVVIGALLTSVTDKNGEEAGKITVGNVLFLLVTTIGYWVYSVFPNVFNKINLGAHSSEGIFLPEVLGILLGALLYLIFTGELKTFKDKEQYLNIIPGLSWGIAAFAYIFAGRGLGTNTAFVLTQLNVIIATFGGILILHEHKSPREMKYTVAGIICIIVGSILTIFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIG6</accession>
    <name>H4GIG6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA gyrase subunit B</fullName>
            <ecNumber evidence="10">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gyrB</name>
        <name evidence="12" type="ORF">PS3_7749</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type II topoisomerase GyrB family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the type II topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="10"/>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121850.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000017.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7749"/>
    <dbReference type="EnsemblBacteria" id="EHS87287">
        <property type="protein sequence ID" value="EHS87287"/>
        <property type="gene ID" value="PS3_7749"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.364"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03366">
        <property type="entry name" value="TOPRIM_TopoIIA_GyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01898">
        <property type="entry name" value="GyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002288">
        <property type="entry name" value="DNA_gyrase_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011557">
        <property type="entry name" value="GyrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034160">
        <property type="entry name" value="TOPRIM_GyrB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00986">
        <property type="entry name" value="DNA_gyraseB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01059">
        <property type="entry name" value="gyrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="11">
        <location>
            <begin position="431"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="647" mass="72325" checksum="84C5BD655F7D8541" modified="2012-04-18" version="1">MSEINETKAEKAREYDASQIQVLEGLEAVRKRPGMYIGSTTAQGLHHLVWEIVDNGIDEALAGFANEINVVVEPDNSITVTDNGRGIPVDIQAKTGKPALETVFTILHAGGKFGGGGYKVSGGLHGVGASVVNALSTELDVRVIREGKEYYMDFDHGHVKTAMKVIDDQRPLTEHGTIVHFTPDPEIFRETTIYNIKTLTERIRELAFLNKGLKITITDKRPDEPTKDEFHYEGGIRHYVEFLNQGHEVLFDPPIYVEGEYKGIAVEVSLQYSDDYRSNLLTFTNNIHTYEGGSHETGFKTALTRVINDYGHKSDQIKGNTTLSGDDVREGLTAVISIKHPNPQFEGQTKTKLSNSDARTATDRIFSETMNRFMMENPEVAHKIVEKGLLASKAREAAKRAREVTRKKSGLEISNLPGKLADNTSKDPAISELFIVEGDSAGGSAKQGRSRLTQAILPIRGKILNVEKATLNRILANDEIRSLFTALGTGFGDEFNIDKVNYHKLIIMTDADVDGAHIRTLLLTLIYRFMRPMLEAGYVYIAQPPLYQVRQGKFVQYIDTDEELEQVVNSLQPSPKPVIQRYKGLGEMDAEQLWKTTMDPENRRLLRVQLDDAKQADEIFQMLMGTKVGPRREFIEANAKFVENLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIG9</accession>
    <name>H4GIG9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease P protein component</fullName>
            <shortName evidence="5">RNase P protein</shortName>
            <shortName evidence="5">RNaseP protein</shortName>
            <ecNumber evidence="5">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein C5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rnpA</name>
        <name evidence="6" type="ORF">PS3_5575</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Consists of a catalytic RNA component (M1 or rnpB) and a protein subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RnpA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121853.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000017.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5575"/>
    <dbReference type="EnsemblBacteria" id="EHS87290">
        <property type="protein sequence ID" value="EHS87290"/>
        <property type="gene ID" value="PS3_5575"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.370"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00227">
        <property type="entry name" value="RNase_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000100">
        <property type="entry name" value="RNase_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33992">
        <property type="entry name" value="PTHR33992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00825">
        <property type="entry name" value="Ribonuclease_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00188">
        <property type="entry name" value="rnpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 5" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="115" mass="13342" checksum="3D528EDEEED8FAAA" modified="2012-04-18" version="1">MRKSYRVKKEQEFQQVFEQHQSVANHKFVIYQIAKPNQPHFRVGISVGKKIGNAVQRGWVKRRIRQTLLEVKPQIKAEVDFLVIARPAAQGMSMAETKRNLVHALKLAHLLTEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIK1</accession>
    <name>H4GIK1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">HPr kinase/phosphorylase</fullName>
            <shortName evidence="13">HPrK/P</shortName>
            <ecNumber evidence="13">2.7.11.-</ecNumber>
            <ecNumber evidence="13">2.7.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">HPr(Ser) kinase/phosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">hprK</name>
        <name evidence="16" type="ORF">PS3_13626</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the ATP- as well as the pyrophosphate-dependent phosphorylation of a specific serine residue in HPr, a phosphocarrier protein of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). HprK/P also catalyzes the pyrophosphate-producing, inorganic phosphate-dependent dephosphorylation (phosphorolysis) of seryl-phosphorylated HPr (P-Ser-HPr). The two antagonistic activities of HprK/P are regulated by several intracellular metabolites, which change their concentration in response to the absence or presence of rapidly metabolisable carbon sources (glucose, fructose, etc.) in the growth medium. Therefore, by controlling the phosphorylation state of HPr, HPrK/P is a sensor enzyme that plays a major role in the regulation of carbon metabolism and sugar transport: it mediates carbon catabolite repression (CCR), and regulates PTS-catalyzed carbohydrate uptake and inducer exclusion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>[HPr protein]-L-serine + ATP = [HPr protein]-O-phospho-L-serine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46600"/>
            <dbReference type="Rhea" id="RHEA-COMP:11602"/>
            <dbReference type="Rhea" id="RHEA-COMP:11603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 13">
            <text>[HPr protein]-O-phospho-L-serine + H(+) + phosphate = [HPr protein]-L-serine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:46604"/>
            <dbReference type="Rhea" id="RHEA-COMP:11602"/>
            <dbReference type="Rhea" id="RHEA-COMP:11603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homohexamer.</text>
    </comment>
    <comment type="domain">
        <text evidence="13">The Walker A ATP-binding motif also binds Pi and PPi.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="13">Both phosphorylation and phosphorolysis are carried out by the same active site and suggest a common mechanism for both reactions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the HPrK/P family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="13"/>
    <dbReference type="EC" id="2.7.4.-" evidence="13"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121885.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13626"/>
    <dbReference type="EnsemblBacteria" id="EHS87196">
        <property type="protein sequence ID" value="EHS87196"/>
        <property type="gene ID" value="PS3_13626"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.410"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006109">
        <property type="term" value="P:regulation of carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01918">
        <property type="entry name" value="HprK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01249">
        <property type="entry name" value="HPr_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003755">
        <property type="entry name" value="HPr(Ser)_kin/Pase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011104">
        <property type="entry name" value="Hpr_kin/Pase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011126">
        <property type="entry name" value="Hpr_kin/Pase_Hpr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028979">
        <property type="entry name" value="Ser_kin/Pase_Hpr-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30305:SF1">
        <property type="entry name" value="PTHR30305:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07475">
        <property type="entry name" value="Hpr_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02603">
        <property type="entry name" value="Hpr_kinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75138">
        <property type="entry name" value="SSF75138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00679">
        <property type="entry name" value="hpr-ser"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 13 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hpr_kinase_N" evidence="14">
        <location>
            <begin position="8"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Hpr_kinase_C" evidence="15">
        <location>
            <begin position="134"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Important for the catalytic mechanism of both phosphorylation and dephosphorylation" evidence="13">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Important for the catalytic mechanism of dephosphorylation" evidence="13">
        <location>
            <begin position="268"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for phosphorylation activity. Proton donor; for dephosphorylation activity" evidence="13">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="157"/>
            <end position="164"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EHS87196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="314" mass="34570" checksum="0BA690BEE95458A7" modified="2012-04-18" version="1">MTDSKTSVTVEELINNARLKILQGAEYIDRPIVTGNICRPALPFAGYFTHYPAERIQLLGITENAFADEVDHEKLAEYCERMCCEETPCFVITSDLPILPELKAAAAKANIPILGTHLTSSRAIGNMSAYLWSQLTPRESVHGVLVDINGTGVMLTGDSGVGKSETALELIRRGHRLVADDRVEVYARDERDLVGSAPAILKHMMEIRGIGIIDVMTLFGTGAVLDEDEVDLIIHLESWSPDKKFDRLGHRGDTRTIQDIAVPQISLPVKSGRNLAIIIESAAMNFRAQNMGYDATTTFDNNLHQLIKQNSKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIK4</accession>
    <name>H4GIK4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Redox-sensing transcriptional repressor Rex</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rex</name>
        <name evidence="9" type="ORF">PS3_3011</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Modulates transcription in response to changes in cellular NADH/NAD(+) redox state.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the transcriptional regulatory Rex family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121888.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3011"/>
    <dbReference type="EnsemblBacteria" id="EHS87199">
        <property type="protein sequence ID" value="EHS87199"/>
        <property type="gene ID" value="PS3_3011"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.414"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051775">
        <property type="term" value="P:response to redox state"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01131">
        <property type="entry name" value="Rex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009718">
        <property type="entry name" value="Rex_DNA-bd_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022876">
        <property type="entry name" value="Tscrpt_rep_Rex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35786">
        <property type="entry name" value="PTHR35786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06971">
        <property type="entry name" value="Put_DNA-bind_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CoA_binding" evidence="8">
        <location>
            <begin position="79"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="7">
        <location>
            <begin position="16"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="90"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="210" mass="23865" checksum="A98ADADBE7A38D77" modified="2012-04-18" version="1">MPNRKIPRATAKRLPVYYRYLNILLNANKKRVSSTELSEAVQVDSATIRRDFSYFGELGKRGYGYDVEKLLNFFKRVLNQDQLTSIALVGVGNIGRALMNYNFHQNTNLRISCAFDPKPELANTIQSGIPVYPVTEMQKQLREQQIDAVILTVPGDQAQKVTDELVETGVRGILNFTPVRLSVPKEVQVQNIDLTNELQTLIYFIESTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIL3</accession>
    <name>H4GIL3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">3-oxoacyl-[acyl-carrier-protein] synthase 2</fullName>
            <ecNumber evidence="3 12">2.3.1.179</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PS3_13763</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in the type II fatty acid elongation cycle. Catalyzes the elongation of a wide range of acyl-ACP by the addition of two carbons from malonyl-ACP to an acyl acceptor. Can efficiently catalyze the conversion of palmitoleoyl-ACP (cis-hexadec-9-enoyl-ACP) to cis-vaccenoyl-ACP (cis-octadec-11-enoyl-ACP), an essential step in the thermal regulation of fatty acid composition.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>(9Z)-hexadecenoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxo-(11Z)-octadecenoyl-[ACP] + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:55040"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10800"/>
            <dbReference type="Rhea" id="RHEA-COMP:14074"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:83989"/>
            <dbReference type="ChEBI" id="CHEBI:138538"/>
            <dbReference type="EC" id="2.3.1.179"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>a fatty acyl-[ACP] + H(+) + malonyl-[ACP] = a 3-oxoacyl-[ACP] + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12 14">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.179" evidence="3 12"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121897.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13763"/>
    <dbReference type="EnsemblBacteria" id="EHS87208">
        <property type="protein sequence ID" value="EHS87208"/>
        <property type="gene ID" value="PS3_13763"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.424"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="PTHR11712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000447">
        <property type="entry name" value="KAS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_KS" evidence="15">
        <location>
            <begin position="4"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000447-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EHS87208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="411" mass="42874" checksum="8934C48B9367D766" modified="2012-04-18" version="1">MTRVVITGMGAIAANGNGYQAFVDNTLAGKVGIKKITKFDASETGITVAGQIDDFDPAQVVGKKEARRMDLYSQYAVQVSQEAIDMAGINEENTNPEDMGVIFGSGIGGLTTIEEQIIKMHEKGPRRVSPFFVPMAISNMAAGNISIRFNAQNMVSSVVTACATATNAIGDAYRQVKEGRAEVMIAGGSEAAVNEIGIAGFAALTALSTNEDPKHASLPFDKDRNGFVLGEGGAALVLESLEHAQARGAKILGEVVGYGSTGDAYHITSPDPSGKGAARAMQQAIKEAGITPDQVDYINAHGTATHANDSGESKSINRVFGEDNHVLVSSTKGMTGHLLGGAGAVEAVATVAGLLQGKLPANVGCFEQDPECQVNLVTKDNQDQPKAEYALSNSLGFGGHNAVLAFRKWSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIM3</accession>
    <name>H4GIM3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Beta-hydroxyacyl-ACP dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_7769</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Plastid</location>
            <location evidence="1">Chloroplast</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-ACP thioesterase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the thioester dehydratase family. FabZ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7769"/>
    <dbReference type="EnsemblBacteria" id="EHS87218">
        <property type="protein sequence ID" value="EHS87218"/>
        <property type="gene ID" value="PS3_7769"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.437"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016297">
        <property type="term" value="F:acyl-[acyl-carrier-protein] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002864">
        <property type="entry name" value="Acyl-ACP_thioesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045023">
        <property type="entry name" value="FATA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31727">
        <property type="entry name" value="PTHR31727"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01643">
        <property type="entry name" value="Acyl-ACP_TE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0150">Chloroplast</keyword>
    <keyword evidence="6" id="KW-0934">Plastid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="252" mass="29054" checksum="8C98E4C1B750434B" modified="2012-04-18" version="1">MEFYEIKHQLNYYECDPAGHPTLSTLINLAVLAAEADNQVNGVGLATVNQFGGGWVIINYAGNLSVHTAHKEEELRIGTRVESYNKFFIIRDFYVKGPDDQIYAEFKGTFVFMDLVKRKIMTIPQAVIDLFDMDPVRRLPRLPQPGDFERSPEWNQQTYRVRYFDIDMNGHVNNAHYFDWMLDPLGGEFLSHHHLVAMRITFAKEVRMDEIITSSVSIPTPLEDGRLMTQHLIEVDGEISAKGENYWIPLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIM9</accession>
    <name>H4GIM9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Recombination protein RecR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">recR</name>
        <name evidence="9" type="ORF">PS3_4595</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May play a role in DNA repair. It seems to be involved in an RecBC-independent recombinational process of DNA repair. It may act with RecF and RecO.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the RecR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121913.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4595"/>
    <dbReference type="EnsemblBacteria" id="EHS87224">
        <property type="protein sequence ID" value="EHS87224"/>
        <property type="gene ID" value="PS3_4595"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.443"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01025">
        <property type="entry name" value="TOPRIM_recR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00017">
        <property type="entry name" value="RecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000093">
        <property type="entry name" value="DNA_Rcmb_RecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023627">
        <property type="entry name" value="Rcmb_RecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023628">
        <property type="entry name" value="Rcmb_RecR_C4-type_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015967">
        <property type="entry name" value="Rcmb_RecR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034137">
        <property type="entry name" value="TOPRIM_RecR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30446">
        <property type="entry name" value="PTHR30446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02132">
        <property type="entry name" value="RecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111304">
        <property type="entry name" value="SSF111304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00615">
        <property type="entry name" value="recR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01300">
        <property type="entry name" value="RECR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="8">
        <location>
            <begin position="80"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="7">
        <location>
            <begin position="57"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="199" mass="22011" checksum="B41A256E7AA78DA4" modified="2012-04-18" version="1">MQYPEPIAKLIDSYMKLPGIGEKSATRLAFYTLGMADDDVKDFALALQAVKQDLHFCTICGNITEDDPCPICKDPTRDQSQILVVERARDIMAMERMNEYHGLYHVLNGTISPSEGTGPMDINIPSLIDRLKQHPEVQEVIIATNASMDGETTAQYLAKLLKPAGIKVTRLAHGLSAGADIDYTDELTLFRAVQGRIEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIN8</accession>
    <name>H4GIN8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative glycogen biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_18579</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial/plant glucose-1-phosphate adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121922.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18579"/>
    <dbReference type="EnsemblBacteria" id="EHS87233">
        <property type="protein sequence ID" value="EHS87233"/>
        <property type="gene ID" value="PS3_18579"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.454"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008878">
        <property type="term" value="F:glucose-1-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011831">
        <property type="entry name" value="ADP-Glc_PPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011832">
        <property type="entry name" value="GlgDAde_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43523">
        <property type="entry name" value="PTHR43523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43523:SF6">
        <property type="entry name" value="PTHR43523:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02092">
        <property type="entry name" value="glgD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transferase" evidence="2">
        <location>
            <begin position="19"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="381" mass="43468" checksum="A4337EEDFF6258C3" modified="2012-04-18" version="1">MKTNSMCAVFDNVYEYNELQPITSFRSLSTLYFAGKYRLMDFPLSSIVNANIREIFMMVNQDKIQSYLDHLGGGKEWGLDTIGSYLEINFAQEMLRKKYEGVRYFDRIINFIRNSGSAYVVYIGNKMLANVDLKAILKFHQVSGNKVTGVFKQVDRWQIAPDDQLFLFDEENKVVGNHRIREMSEQEQYNLSMNVYIADADWFVDVLDRAQMSGIVPDATEGLARLMTHEHSSAYEYTGYMQNIHDVKSYFKANMDMLEKTHYDSLLNGSQKVITRIKNEVGNFYTKTSQVSQSLVSTGCKVAGHLDHVVVSRRVKIEEDTQIDHSIVISNAVIKRGAQISYAIIDKNVVVEPSVVIKGTPERPVVIRKDQVVTQDVIEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIP6</accession>
    <name>H4GIP6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UDP-N-acetylglucosamine 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Enoylpyruvate transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">UDP-N-acetylglucosamine enolpyruvyl transferase</fullName>
            <shortName evidence="7">EPT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">murA</name>
        <name evidence="9" type="ORF">PS3_9746</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:18681"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="EC" id="2.5.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the EPSP synthase family. MurA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.7" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121930.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9746"/>
    <dbReference type="EnsemblBacteria" id="EHS87241">
        <property type="protein sequence ID" value="EHS87241"/>
        <property type="gene ID" value="PS3_9746"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.465"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008760">
        <property type="term" value="F:UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019277">
        <property type="term" value="P:UDP-N-acetylgalactosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01555">
        <property type="entry name" value="UdpNAET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00111">
        <property type="entry name" value="MurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005750">
        <property type="entry name" value="UDP_GlcNAc_COvinyl_MurA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01072">
        <property type="entry name" value="murA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 7" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EPSP_synthase" evidence="8">
        <location>
            <begin position="7"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoenolpyruvate binding" evidence="7">
        <location>
            <begin position="22"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-N-acetylglucosamine binding" evidence="7">
        <location>
            <begin position="121"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="420" mass="45247" checksum="6C2AC437F7185EED" modified="2012-04-18" version="1">MKKMIIKGGNRLAGEVTIGGAKNSTVALIPAAILADTPVQFDTVPDILDVHNLMIILESMNVKSSFTHGVLDVDPTQIIEAELPSKAIKSLRASYYFMGALLGRFHRATVTFPGGDNIGPRPIDQHLKAFMALGATVSEDNGTVHLAAPQGLHGARIFMDMVSVGATINAILAAVRAEGTTVIENAAREPEIIDLATFLNNMGAQIRGAGTDVIRITGVQTMQSMNTHTIIADRIETGTYLSLAAAMGDGVMINNVIPEHIEAYTSKMIDMGVDLQIGIDRIYVPTVHDLKPTIVKTMPYPGFATDLQQPLTPLMSRAAGESRVVDTIYPKRVKHIPELQKMGMDISAQDGSILVRQSQQLRGADVKAAEIRAGASLMIAGLMAEGTTTIHNADHILRGYDRIVWKLNRLNADVAIEDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIP8</accession>
    <name>H4GIP8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable DNA-directed RNA polymerase subunit delta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">RNAP delta factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpoE</name>
        <name evidence="9" type="ORF">PS3_7303</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Participates in both the initiation and recycling phases of transcription. In the presence of the delta subunit, RNAP displays an increased specificity of transcription, a decreased affinity for nucleic acids, and an increased efficiency of RNA synthesis because of enhanced recycling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">RNAP is composed of a core of 2 alpha, a beta and a beta' subunits. The core is associated with a delta subunit and one of several sigma factors.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the RpoE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121932.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7303"/>
    <dbReference type="EnsemblBacteria" id="EHS87243">
        <property type="protein sequence ID" value="EHS87243"/>
        <property type="gene ID" value="PS3_7303"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.467"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00357">
        <property type="entry name" value="RNApol_bact_RpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007759">
        <property type="entry name" value="Asxl_HARE-HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038087">
        <property type="entry name" value="RNAP_delta_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029757">
        <property type="entry name" value="RpoE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05066">
        <property type="entry name" value="HARE-HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04567">
        <property type="entry name" value="RNAP_delt_lowGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51913">
        <property type="entry name" value="HTH_HARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HTH HARE-type" evidence="8">
        <location>
            <begin position="14"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="90"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="117"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="182" mass="21032" checksum="D76B5AF6693B26ED" modified="2012-04-18" version="1">MELHVFDGQDKSELSMIEVAHAILSFNGEAMAFADLTNEIQQYLDKSDEEIRERLSQFYTDLNVDGSFISLGDNTWGLRAWYPYDSIDEATVGETDDEEDRPKKKRRKVNAFLAGSDDDDDVIDYDNDDPEDEDLDDDDVDDDDYDDDYDDDNPDEDDDEDTNIEDQLSEFSDDELEDDEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIQ0</accession>
    <name>H4GIQ0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_8649</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121934.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8649"/>
    <dbReference type="EnsemblBacteria" id="EHS87245">
        <property type="protein sequence ID" value="EHS87245"/>
        <property type="gene ID" value="PS3_8649"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.470"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000150">
        <property type="entry name" value="Cof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00099">
        <property type="entry name" value="Cof-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="272" mass="29440" checksum="0FA7C94FF9BE84D7" modified="2012-04-18" version="1">MAIKLVAIDIDGTLITDQLTITPATKEAIQQATKQGVKVVLCTGRPMTGVSSYLDQLELNGLSDEYVISFNGGLAQNTAGDQLAKFVIPFDDMIDLGSFALKHALHMVVETSHHIHSINRQISPFSTNESNLVSLPLQYSSLDALADCRQDLTISKLMFTDDAHLIDQAIEDLPEELTSRFNIVRSEPYYLEFVNPKASKGAALKLLGEELGIQADEMMALGNAQNDESMIEFAGLGVAMENSIPSTLAIADAVTASNNHDGVAKAIEKYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIR8</accession>
    <name>H4GIR8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_11893</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121952.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11893"/>
    <dbReference type="EnsemblBacteria" id="EHS87263">
        <property type="protein sequence ID" value="EHS87263"/>
        <property type="gene ID" value="PS3_11893"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.488"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027785">
        <property type="entry name" value="UvrD-like_helicase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13538">
        <property type="entry name" value="UvrD_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="7">
        <location>
            <begin position="210"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="17"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="231"/>
            <end position="238"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="767" mass="88674" checksum="EA40C26667357CE1" modified="2012-04-18" version="1">MTDAERQIEQSRVDQVIKEIQLQINATRTELAKAHAETRAVEENYGANASINRYEIDDIAESRSAIEQQRQLVARATENEAILQHQLTTLEQLAKQPYFGRIDILDPGEREPESLYIGTASLMDHTKSEFLIYDWRAPISGIYYNGTLGEVSYQTPAGLQNTKLVKKRQFTIIDGQITNMFDTNETVGDEMLQAVLGEQDNQAMRNIVATIQHDQNTIIRDTESDLLVVQGVAGSGKTSTILQRVAFLLYHARNQLNADQMVLFSPNQLFSHYISQVLPSLGERNLRQVTLHNFLQRRFEGLQVESLFERYEERQLDRSHQASDDFFESPASMAAVQDYLALIRQHQRSLQFIDVTFNGRPFFSQKHFQHIWDQIDPQLKLQDQLVKLKNHFIRLLKQRITKIAQSDWLTKELDTLNRQQIQRLLGSKSIDDFEDTAAYDRYLARQLAKQRLRIVYDALYNNLMIDFPNQYTDFLQWIDLPVAIDRQALIQSFQHQLEFHHFPLSHTAPYLYLRDLLTGRGQNRNFQYVFIDEMQDYPLSLLIYFKHTFPKAQFTVIGDSEQALFRPLEAPQVLLKRVSKSLQAQHSTLVNLQQSYRSTYEITKLATSLLPDGDQITAFSRHGTQPQVIIRHDSTAWQTALIKSAQRLQNDYQTVAILSQDDLQAQALYHLLHPHFGQLSHLTEHDLSLKTGIIILPIYLAKGLEFDAVIIPDISQDHFTRQQTGQLYTMMTRAMHELFMLSLGPITTLITDSQHLQIDQQISFKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIS0</accession>
    <name>H4GIS0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Na-H antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_17151</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121954.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17151"/>
    <dbReference type="EnsemblBacteria" id="EHS87265">
        <property type="protein sequence ID" value="EHS87265"/>
        <property type="gene ID" value="PS3_17151"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.490"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006812">
        <property type="term" value="P:cation transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="216"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="291"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="360"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Na_H_Exchanger" evidence="5">
        <location>
            <begin position="9"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="388" mass="41263" checksum="AB037C9FF66D41C5" modified="2012-04-18" version="1">MSFIGTLALILLVTLIVSQLFSRVGIPDVIGQILVGVVAGPAMLGWLQQSEMIEQFQEIGVIVLMFIAGLESDLSLLKKYLKPALMVALGGMLLPILVMGVAAYGFGIPVFEAFFIGVVFSATSVSISVAVLREYRKMDTKEGATILGAAVADDIGGVMLLSIMISMMNSQGSHVSGHGSTNLGLVLLLQVAFFGGSYLLIRWLAPYLMHLSSKMLPIAANSVMAMVICLGMAAAAESVSLSGAVGAFFAGIAIANTHKKETVNRSFIPIGYAMFIPIFFVNVGLSMRLDHFWDSIVFILVMTVLACLTKLLGSGGGAKMMGFPLDSSYVIGSGMIARGEMALITAQIGYEAHLLSESYYSDIITVIVLATILAPFILKHAIKRMKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIV2</accession>
    <name>H4GIV2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">30S ribosomal protein S3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rpsC</name>
        <name evidence="12" type="ORF">PS3_15042</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the 30S ribosomal subunit. Forms a tight complex with proteins S10 and S14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 10">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121985.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15042"/>
    <dbReference type="EnsemblBacteria" id="EHS87182">
        <property type="protein sequence ID" value="EHS87182"/>
        <property type="gene ID" value="PS3_15042"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.534"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_B">
        <property type="entry name" value="Ribosomal_S3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005704">
        <property type="entry name" value="Ribosomal_S3_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_S3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_S3_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="SSF54821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01009">
        <property type="entry name" value="rpsC_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 8 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="11">
        <location>
            <begin position="38"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="214" mass="23846" checksum="EAE8AC173C9EE7E4" modified="2012-04-18" version="1">MGQKVNPTGFRVGVIRDWTAKWYADKDYAAYLNEDLRIREYIEKRLADASVSTVEIERAANRVNVSIHTAKPGMVIGKGGSEVEALRKELNALTGKRVHINVVEIKKPDLEAHLVGESVAQQLENRVAFRRAMRGAMQRAMRAGAKGIKIQVAGRLNGADMSRVESYSDGTVPLHTLRADIDYSWDEAHTTYGVLGIKTWIYRGEVLPAKKNKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIW9</accession>
    <name>H4GIW9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Putative sucrose-specific phosphotransferase system (PTS), IIABC component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PS3_16689</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122002.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16689"/>
    <dbReference type="EnsemblBacteria" id="EHS87141">
        <property type="protein sequence ID" value="EHS87141"/>
        <property type="gene ID" value="PS3_16689"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.560"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00212">
        <property type="entry name" value="PTS_IIB_glc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036878">
        <property type="entry name" value="Glu_permease_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018113">
        <property type="entry name" value="PTrfase_EIIB_Cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001127">
        <property type="entry name" value="PTS_EIIA_1_perm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003352">
        <property type="entry name" value="PTS_EIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013013">
        <property type="entry name" value="PTS_EIIC_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011297">
        <property type="entry name" value="PTS_IIABC_b_glu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001996">
        <property type="entry name" value="PTS_IIB_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00358">
        <property type="entry name" value="PTS_EIIA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00367">
        <property type="entry name" value="PTS_EIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02378">
        <property type="entry name" value="PTS_EIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55604">
        <property type="entry name" value="SSF55604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00830">
        <property type="entry name" value="PTBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01995">
        <property type="entry name" value="PTS-II-ABC-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51093">
        <property type="entry name" value="PTS_EIIA_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00371">
        <property type="entry name" value="PTS_EIIA_TYPE_1_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51098">
        <property type="entry name" value="PTS_EIIB_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01035">
        <property type="entry name" value="PTS_EIIB_TYPE_1_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51103">
        <property type="entry name" value="PTS_EIIC_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="218"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="295"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="390"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="435"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIB type-1" evidence="15">
        <location>
            <begin position="7"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIC type-1" evidence="16">
        <location>
            <begin position="124"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIA type-1" evidence="14">
        <location>
            <begin position="519"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate; for EIIB activity" evidence="11">
        <location>
            <position position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EHS87141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="634" mass="67672" checksum="CD0BD4932F491316" modified="2012-04-18" version="1">MAKKDFKPLATDIIKEVGGPDNINNVIHCITRLRFYLHDESLADDDAIKAHDDVIDVIHASGQYQVVIGNEVGRVFDAVTAQLGDNLEPTPTTTENDDRNPIVRGFGNLIGFITGSMSPVIGVIAASGIIKGILALLTLPQLGALLDTESTTYITVSAMADSAFYFLPILVGFAAARRLGSDPIVAAVIGGVLTYPQIVTWGGKFKEMFAIDGFKFEFLNYTYSIFPMILAAWLAKKLGDWLKDHLPSYLQMIFNPLITVLVVSTITLVITGPIIQGFANGIADLINALVHASGWLGGLIIGGFYQLLVIFGLHWGVVPLVTQQISQSGESALNAIISSTMVAQGAAVLAVALKTKIKKRKSLGIAAAISAFCGVTEPAIYGINLRFRRVFVSGMFGSAAGGLITGLMGGTMYGFTGSLIGFSSFFNPKHPTQLTSFYAFLLSSLAALVVSFVIAWVWGYNDDMEMGEKVEKKQRPGSQKSPAQVVKETTVPDPDARVSEETLASPVAGEIIPLSSVNDELFSKGTMGQGFAVTPTDGHVVAPVDAEVVVAYETGHAIGLTTHDGAEVLIHIGLDTVMLNGKGFDMKVKQGQMVKAGEPLVDFDLEQIKAAGYDPTVIVVVTNPRNVDVNFPTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIX1</accession>
    <name>H4GIX1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_20236</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122004.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20236"/>
    <dbReference type="EnsemblBacteria" id="EHS87143">
        <property type="protein sequence ID" value="EHS87143"/>
        <property type="gene ID" value="PS3_20236"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.563"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="PTHR43827"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="4">
        <location>
            <begin position="25"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="3">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="286" mass="32479" checksum="53E90CBA74228EF4" modified="2012-04-18" version="1">MQELTSLRSPLKLANGVEIPGLGYGTYKSSNEEVTQAVIDALAAGYRHIDTAAYYGNQKGIGEALRQTDLSREDIFITSKVWNTDRGYDQTMAAFEKTIAELGVDYLDLYLIHWPANRKQFGDQARKLNADTWRALENLYQAGRVRAIGVSNFMPNHLLELMADSRIKPMVDQIEYHPGWPQEATRRFCQRHGILVEAWRPLGTKVALENPLIQEIADKYQHSPAQVCLRWSIQQGLLPLPKSVNPERMKANADIFDFELTEDEMDVISSIRIVSAKNQIPDQVDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIZ8</accession>
    <name>H4GIZ8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Penicillin-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_21136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21136"/>
    <dbReference type="EnsemblBacteria" id="EHS87110">
        <property type="protein sequence ID" value="EHS87110"/>
        <property type="gene ID" value="PS3_21136"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.603"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase" evidence="1">
        <location>
            <begin position="13"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="326" mass="36751" checksum="73B58CDDF3EE1565" modified="2012-04-18" version="1">MTNKNPVTSQDTRTAIKQILANHQVNGVVVVNGKDNQPLVITNQITGVDQAHTMTGERLFPIASLNKLITGIAIQQQLNRGKLSLNDKLSQYYPKVTNSNQITIRDLITHTSDLQDTSQASSQVMKKQSTRLNYTLDHYLATGNFTWAYSNTDYVLLAGILSKLTHQSYHQYVQRHFIKPLKLKQTKYFDQVKANQVALPVGESNLQANLINLQKNMSVTLGAGDYFFSAKDYWRFVNAVGHNKFVKMSSIDQNVDFTTGTDHYFDGVYFRAGKLFVANGCYGNLCSSVYCNYGNHQTMVVFANNIPYNQEKLIASQISQVYFNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ01</accession>
    <name>H4GJ01_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FMN-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_4929</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122034.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4929"/>
    <dbReference type="EnsemblBacteria" id="EHS87113">
        <property type="protein sequence ID" value="EHS87113"/>
        <property type="gene ID" value="PS3_4929"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.608"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007329">
        <property type="entry name" value="FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04205">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00900">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_bind" evidence="2">
        <location>
            <begin position="67"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="148" mass="15628" checksum="BF5CA01279B8F5B2" modified="2012-04-18" version="1">MFKKFIALILSLAVAGAIIVDGYLLFFKKTTTNTKVSTESTGNQSTTSSSTNSTMKDGTYTGASTSTAWGDVQVKMTVANGKITSITVLKHPTEGKSVQINAESLPIYKKEAIAAQSAKIQQVSEATETYKGFTGSLQSAITQAEEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ02</accession>
    <name>H4GJ02_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_5697</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5697"/>
    <dbReference type="EnsemblBacteria" id="EHS87114">
        <property type="protein sequence ID" value="EHS87114"/>
        <property type="gene ID" value="PS3_5697"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.609"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="759" mass="85982" checksum="3FDCACF39D61FBCE" modified="2012-04-18" version="1">MNHINVLLNNQPQTMAIEEIFDPIAYPQLQAVTGKVSADFANQYLADYIKVDLVLGNLPSASNFSANETLTKDALLTALLTVANKDAAKLFQALSSTVQLNALAGIFRVLISPTQAIHSRFYLMYNPSTDQKRVVLGSFDLTETAFDPAYNRFEEVIVFDSAELYDQLSAHFSKDLLPTTKAYFTDELLAAAEAQLKALKQDQVAQGQKVVIFSNEETDQIVQAELTNLLVKNVQKQIDADLIPADTPLAMRNVTMDRTREREAEERLIQQRDMMLQLEKEAVSPRAEQPSLRSADQVAKKVKAALAEAISPEDLAVEKKYTTFLYDRPIERNVAKNITGLYIPSDTGQYPIPFGKLATVEQIKSGLQQIDAVMNGYQQYVVDYDDNYGKRFFEAILYGFTAPFMWEIRTKASLNPEDGNDVPNFLILGATAGSGKSTLLRIINQLTWGNENSMIDFGTIYPNETPQRKAKTVQALESYMKQGSTYPVLVDEIEPYFFQQPQYSRHLVVDTMNELVNSPKAIAPLIGTTNYNGSFSMVRETARRTYYIQLDKVINDERKEEATQYIFDVRQSLNNTLFKDFVVRMAHRLADDDTNWRTFDATNGQLDFLAITRDIFKEYYRMAKMSVPAYVSDGLCDDFRESSRNKWAKLYLTQMDDFVFRSENNSLVFDITKLNSFNGFSNESAEEYRNSLPIEICVDGINGKSGKFLEMKADDFFNWIGIKPELTAITHENGTTKVTTKVADEKPKKKKGFWARLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ16</accession>
    <name>H4GJ16_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Small heat shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_19984</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000027">
        <property type="protein sequence ID" value="EHS87092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122049.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000027.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19984"/>
    <dbReference type="EnsemblBacteria" id="EHS87092">
        <property type="protein sequence ID" value="EHS87092"/>
        <property type="gene ID" value="PS3_19984"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.636"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="30"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="143" mass="16578" checksum="AB2F9509BA920B42" modified="2012-04-18" version="1">MANEIQRRNDLFGDIFDMRNWMNDSFFSKMANNDGHMRTDIAETEKNYRVKVDMPGFQKKDIHINYANNILTITGARDTFDDVSDKDGNILHSERRYGQMSREFRLPDVDINDASAKYEDGVLILTLPKLDAVNDSDTHIEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ18</accession>
    <name>H4GJ18_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glutamine ABC transporter substrate binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_3015</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial solute-binding protein 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000027">
        <property type="protein sequence ID" value="EHS87094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122051.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000027.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3015"/>
    <dbReference type="EnsemblBacteria" id="EHS87094">
        <property type="protein sequence ID" value="EHS87094"/>
        <property type="gene ID" value="PS3_3015"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.639"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018313">
        <property type="entry name" value="SBP_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01039">
        <property type="entry name" value="SBP_BACTERIAL_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003591868" evidence="4">
        <location>
            <begin position="33"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="5">
        <location>
            <begin position="46"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="282" mass="31619" checksum="FF92FB4DDE06F141" modified="2012-04-18" version="1">MKKMKQFLALLCLGIVGVLLVGCQKQSVTQKANTQDTWAKIKKRGTLLIGVDDSFVPMDFRQKNGQLVGYDVDLTKAVCKKLGLKAEFQSIDWSMKETELRNGTIDVIWNGYTRTKARAKKVAFSRVYERSGQALVVRKDSGINKFSDMKGKTLGVQQGSTAYTDLLKYPKLLKQYIKNGTPIIYNDNNSSFLDLKAGRIQGVLTGSVYAGYYATHLDKNKYKLIGLDVFPSDQVAVGMRKGDRTLLKKINWALGELQKDGTLRKINQKWLGIDDDYLGPVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ41</accession>
    <name>H4GJ41_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="3 11">2.4.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">UMP pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">UPRTase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">upp</name>
        <name evidence="12" type="ORF">PS3_9423</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>diphosphate + UMP = 5-phospho-alpha-D-ribose 1-diphosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:13017"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 1 Mg(2+) ion per subunit. The magnesium is bound as Mg-PRPP.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Allosterically activated by GTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UPRTase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="3 11"/>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122074.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9423"/>
    <dbReference type="EnsemblBacteria" id="EHS87066">
        <property type="protein sequence ID" value="EHS87066"/>
        <property type="gene ID" value="PS3_9423"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.680"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00636"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006223">
        <property type="term" value="P:uracil salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01218_B">
        <property type="entry name" value="Upp_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034332">
        <property type="entry name" value="Upp_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005765">
        <property type="entry name" value="Ura_phspho_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01091">
        <property type="entry name" value="upp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="5 11 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11 12" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="5-phospho-alpha-D-ribose 1-diphosphate binding" evidence="11">
        <location>
            <begin position="131"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Uracil binding" evidence="11">
        <location>
            <begin position="201"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>uracil</name>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="211" mass="23165" checksum="65AAB68A1AB2B8F3" modified="2012-04-18" version="1">MAKFEVMDHPLIQHKLTMIRDKNVGTKVFRETVREISTLMAYEVSRTMPLKDVEVETPICKTTKKSLAGKKVAIIPILRAGLGMVDGITEMIPAAKIGFIGMYRDETTLKPHEYFVKLPEDIAERQLFIVDPMLATGGSAVMAIDALKKRGAKETAMKFVSLVAAPEGVKAIQEAFPDVDIFTAALDDHLNEDGYIVPGLGDAGDRLFGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ44</accession>
    <name>H4GJ44_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptide chain release factor 1</fullName>
            <shortName evidence="5">RF-1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">prfA</name>
        <name evidence="7" type="ORF">PS3_13435</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="5">Methylated by PrmC. Methylation increases the termination efficiency of RF1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122077.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13435"/>
    <dbReference type="EnsemblBacteria" id="EHS87069">
        <property type="protein sequence ID" value="EHS87069"/>
        <property type="gene ID" value="PS3_13435"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.683"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00093">
        <property type="entry name" value="Rel_fac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004373">
        <property type="entry name" value="RF-1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00019">
        <property type="entry name" value="prfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0488">Methylation</keyword>
    <keyword evidence="4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RF_PROK_I" evidence="6">
        <location>
            <begin position="231"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="5">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="362" mass="41224" checksum="8B2272ECF4643571" modified="2012-04-18" version="1">MEISEVFDKLQAVADRYEELNELISDPEVIADTQRFMKLSKEEGSLRETVEKYNRYKEVTQTIDDDEELLRETNDPELLELTKEELSDAKLEQSQLQDELEVLLIPKDPNDDKNIIMEIRGAAGGDEASLFAGDLYNMYVRYAENQGWQVEVVDQTATEVGGFKEIVLMITGDNVYSKLKFENGAHRVQRVPVTESAGRVHTSTATVGVMPEAEDVEIDLDDKDIRVDVYRSSGAGGQHINKTSSAVRMTHLPTGIVVAMQDERSQQQNRAKAMRILKSRVYDYYQQQEQSEYDANRKKAIGTGDRSERIRTYNFPQNRVTDHRIGLTLNKLDKVLAGDLDEIIEALIIADQTEKLEQLRNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ71</accession>
    <name>H4GJ71_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_5165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5165"/>
    <dbReference type="EnsemblBacteria" id="EHS87026">
        <property type="protein sequence ID" value="EHS87026"/>
        <property type="gene ID" value="PS3_5165"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.719"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="9"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="218" mass="23715" checksum="1A96304F49B8E489" modified="2012-04-18" version="1">MMLKVGIIGATGQAGSELTKAAQASGQIEVTALVRNGEKAKTMLGDQVKVLEKDAFALTKADLADFDVIVDAFATAPDQSYLQVDLAAKLVSFFRDTTSPRLVFILGAGSLQTGDDHHLFVDDIAKTHGANEWIAIPQNQLAELEFLQNVKNVNWVGVSPGAEFKVGPKATAVKYGHDELLFNDQGESFTTYATMGEVLTKEIIEPQYHQERFTVINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ75</accession>
    <name>H4GJ75_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_4925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122108.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4925"/>
    <dbReference type="EnsemblBacteria" id="EHS87030">
        <property type="protein sequence ID" value="EHS87030"/>
        <property type="gene ID" value="PS3_4925"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.728"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="16"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="327" mass="36213" checksum="C46B8173B5E2A1E4" modified="2012-04-18" version="1">MTKSVTIGKTDVTTTPLGLGTNAVGGYNLFPNLKDEDGIKLVQAGIDHGIRLLDTAYVYGLGHSEELVGQAIKNYDRHELTIATKGAHDFSTGEEVINNDPKFISQQVDQSLQRLGTDYIDIYYLHFPDHDTPKAEAVGALQRLREQGKIRTIGISNFSLDQIKEANADGYVDVVEDEFSLLHQDHLADGMLEYLHENQISFVPYFPLASGLLTGKYDHLTEFPADDIRSQIADFKEPRFTKALAAVDQIRSIADKHGATVAQTVLAWHIQNPFISVVIPGAKRPDQVAANAKALDLELSLEEYQTIEQAFTWFKQTQSGKSLADPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ83</accession>
    <name>H4GJ83_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Copper-translocating P-type ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_6973</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122116.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6973"/>
    <dbReference type="EnsemblBacteria" id="EHS87038">
        <property type="protein sequence ID" value="EHS87038"/>
        <property type="gene ID" value="PS3_6973"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.740"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="581"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="610"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="638" mass="68779" checksum="B10D4AAAE4B27A6A" modified="2012-04-18" version="1">MTYTKRFFISLVLAIPMLIQMIGMIFNWMMPFYNWIAFITTTLIMALSALPFWQSAWAAFKHHHANMDTLVAVGTAVAYFYSIFAMFTGREVYFESAAFVTVFVLLGQVFEERMRHNASSAVGKLINLQTKTADVIRDGEVMTIALDQVAVGDLVQVRPGQKIPVDGVVTEGRSTVDESMLTGESMPVTKEMGDQVYGATLNKSGRLLLKVQKTGNATMLAQIVELVKMAQTSRAPIQKLTDRIADVFVPLVLIIAIVTFTLWYLLFGTTLVQAMLYGVAVIVIACPCALGLATPTAIMVGTGRAAKYGVLIKDGEVLEKVDHLKTIVFDKTGTITIGQPQVTDVIGDRQMTLQLAASLENSSEHPLAQAILEIAKKDGVPQQSVNNFQAIPGRGVRGEIDQQTILVGNDKLVASQQISADLQKAKVQLHQEAKTVVNVGVDDQVIGLIAIQDQPKADADQAIKALKQRGLKTMMLTGDNQEVAHSIAQLVGIDQVIADVLPDQKEASIRHLQATGSVAFVGDGINDAPALSTADVGIAMGSGTDIAIEAGDIVLTNNRLLGVVDAIDLSKKTFQRIKLNLFWALIYNVIGIPIAAGIFAELGLILSPEFAGLAMALSSISVVGSSLLLNRVKVGYEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ84</accession>
    <name>H4GJ84_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cupredoxin_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_12215</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122117.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12215"/>
    <dbReference type="EnsemblBacteria" id="EHS87039">
        <property type="protein sequence ID" value="EHS87039"/>
        <property type="gene ID" value="PS3_12215"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.742"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028096">
        <property type="entry name" value="EfeO_Cupredoxin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13473">
        <property type="entry name" value="Cupredoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="Cupredoxin_1" evidence="2">
        <location>
            <begin position="15"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="125" mass="13726" checksum="BA71B752544AD5A7" modified="2012-04-18" version="1">MSVLQILVLILAIVLIAFISWWFFGKHDTGAVSATMVGGHQQVEIKVDGGYSPETVVLKQGVPAQLIFNRLDKSNCLDEVVFPDQGIDEKLPAEAVKKIPFNTDQAGEYSYACGMNMFHGKVIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJA8</accession>
    <name>H4GJA8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_12626</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000032">
        <property type="protein sequence ID" value="EHS86998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122141.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000032.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12626"/>
    <dbReference type="EnsemblBacteria" id="EHS86998">
        <property type="protein sequence ID" value="EHS86998"/>
        <property type="gene ID" value="PS3_12626"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.801"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00036">
        <property type="entry name" value="HTHLACI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="3"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="329" mass="36346" checksum="471633FEBAA97187" modified="2012-04-18" version="1">MTATIKDVANLAGVSTATVSRVLSDKKGTYSQQAAQKVLAAAKKLGYVKNVAAAELVSKSSNRIAVILNNTLTNFWTDVIKGIYDAAMKMGYQIFILYAGDKNLPQFHNALDVALERPLAGILMISTMIDQKAKDRLQASGVPFRLVSIYDEKKRLYKDLPYTSSNNVEIATIATRYLIELGHERIGLVGIDRSNTGQQRLFGFEQEMIKHQLPIEPSWIHYGNYSYPTGQNSFADLLNDQLTAVITASDMIAVGMIQQANVSGITLPQQLSILSIDGTYLCEITTPTITSITQNFYQMGRQSVIGLLNDQPAEFIPVEITERQSTIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJB8</accession>
    <name>H4GJB8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_6948</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000033">
        <property type="protein sequence ID" value="EHS86987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6948"/>
    <dbReference type="EnsemblBacteria" id="EHS86987">
        <property type="protein sequence ID" value="EHS86987"/>
        <property type="gene ID" value="PS3_6948"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.818"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08298">
        <property type="entry name" value="CAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014187">
        <property type="entry name" value="ADH_Zn_typ-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02822">
        <property type="entry name" value="adh_fam_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="3">
        <location>
            <begin position="20"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="341" mass="36399" checksum="5E208094AC4411DD" modified="2012-04-18" version="1">MSNTNTMLAWHLTKPGPVDQSPAPIELIECPIPTPATNEVLVKVLTCGVCHTDLHVTEGDLPMHQEHVTPGHEIVGEVVANGSSAHRFQKGDRIGIPWFRQACGVCRFCRSGQENLCPHSTYTGWDHDGGYAQYVTVPEGFAYRLPMELDPVSAAPLLCAGIIGYRAFKRAQVPAGGSLGLYGFGGSASLCAQLALHEGIEVHVFTRGLAAQAAALKMGCASAQGAYDPAPKLLDSAILFSPAGEMVPNALTNLAPGGTLAIGGIHLSDIPSLNYQAAIFHEKNLTSVESNTRQDGEEWLALAHRLKIIPHTTEYPFDQALVALQRVKKGNLDGALVLKMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJC1</accession>
    <name>H4GJC1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Biotin carboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_6505</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000033">
        <property type="protein sequence ID" value="EHS86990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122154.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000033.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6505"/>
    <dbReference type="EnsemblBacteria" id="EHS86990">
        <property type="protein sequence ID" value="EHS86990"/>
        <property type="gene ID" value="PS3_6505"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.822"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="8">
        <location>
            <begin position="16"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="7">
        <location>
            <begin position="135"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS86990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="456" mass="50429" checksum="70A01C423D1EB1E7" modified="2012-04-18" version="1">MDNHLFKLSDSVSKWPFHKVLVANRGEIAVRIIRTCRVLGLATVAVYSDADSQALHVKLADEAVKIGPANASQSYLNIAALMAAVQLTGADALHPGYGFLSESPQLARACQENHIKLIGPSAELMELLGDKERARQTMQRAGLPIVEGSQSIITDLSTAQQQAEQIGYPIMLKAAAGGGGKGMRILHSAEELNQQFATAQDEAMASFDDRRMYMERYLAHPRHIEVQIIADRYGHAIALSERDCTIQARHQKVIEEAPAVVIPEAIRSEMLSRCEVAVAQLHYEGVGTIEFLYNEDGSYYFMEMNTRIQVEHAISEQVNLVDLIELQLRLAAGGLLSGQRPSTQGFALECRINALTPGKITALHLPGGQGVRVDTAIYQGYQVPTNYDGMIAKLIVYGDRRQRVLKQMQTIVDETVIEGVQTNLGELAQILREPDFQRLKTTVNWLDELQSQEVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJD0</accession>
    <name>H4GJD0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Flavoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_10758</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000034">
        <property type="protein sequence ID" value="EHS86971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122163.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000034.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10758"/>
    <dbReference type="EnsemblBacteria" id="EHS86971">
        <property type="protein sequence ID" value="EHS86971"/>
        <property type="gene ID" value="PS3_10758"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.834"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="FMN_red" evidence="2">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="255" mass="28722" checksum="96358B3035FBCD1E" modified="2012-04-18" version="1">MKIVGIFGAHQASGLTKQMLNALWAGIPHDIETTTIDLNQLVIQPDIPGQANPVLDEIERALKESDIWVIAAPTYWGGLSGEMKHFFDCIRPRIVRFDHRGGMHPGQYRDKHYVTLTNCYASKWTNLTDGITNATFNQVDKVMTTAGLHKAGEVVLTDTWELTKLPNKKAAQLKNLGQKLGQLQRKDDETMKRYLLLFAVVAIMTLITMGIQALISWAFHWQAGFWGNYIAFVCIFFAMLSISLHLLTVSLHARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJG0</accession>
    <name>H4GJG0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Choloylglycine hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_3325</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C59 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000037">
        <property type="protein sequence ID" value="EHS86721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122193.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000037.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3325"/>
    <dbReference type="EnsemblBacteria" id="EHS86721">
        <property type="protein sequence ID" value="EHS86721"/>
        <property type="gene ID" value="PS3_3325"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.910"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029132">
        <property type="entry name" value="CBAH/NAAA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02275">
        <property type="entry name" value="CBAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBAH" evidence="3">
        <location>
            <begin position="2"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="305" mass="35299" checksum="2E2DB4C8FB9D704C" modified="2012-04-18" version="1">MCTAITYQDHGKNIFGRTMDLPTLNTPWELTYLPSNYQFTRYTDQTKFQSKYAILGGMRQTNGKFLIGDGINAAGLVIAELYLPNLPQYHQQIQPNKINLAPQDVTEWVLANYDSVASVQADLANWALVASKWYDDAIIHQFHWMLVDQTGTYLIEPTALKLEIRKLDIPVITNTPRYDDHLRRLANYINLDADQDFKTTLKEFAGDLPINPRTPTQRFQYAAIRQAKLQSAFTPDDLNQILQAVKMEHLPGHEDYTHYIGTIDLVSQTYVYHDVDRHQTKRVHLDQLTPLKSPYIFHPRDYKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJG6</accession>
    <name>H4GJG6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Appr-1-p processing domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_14024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000039">
        <property type="protein sequence ID" value="EHS86609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122199.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000039.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14024"/>
    <dbReference type="EnsemblBacteria" id="EHS86609">
        <property type="protein sequence ID" value="EHS86609"/>
        <property type="gene ID" value="PS3_14024"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.917"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="SSF52949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="171" mass="18458" checksum="9E5561440FF194C0" modified="2012-04-18" version="1">MVALTVKLGDITQFKVDAIVNAANTTLRGGGGVDGAIHRTAGPALDLAFAQLGGCPTGQARITPGFNLPAKYIIHTPGPIWVDGHHQEAELLADSYRNSLQLALSYGCQTVAFPSISTGVYHFPLERAATIAVETMFDFLGNQTTIKQVWIVAFDPVTAKVYRNKIDEFQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJH0</accession>
    <name>H4GJH0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_8982</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000039">
        <property type="protein sequence ID" value="EHS86613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8982"/>
    <dbReference type="EnsemblBacteria" id="EHS86613">
        <property type="protein sequence ID" value="EHS86613"/>
        <property type="gene ID" value="PS3_8982"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.921"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007438">
        <property type="entry name" value="DUF488"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04343">
        <property type="entry name" value="DUF488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="120" mass="14029" checksum="983CC2C29F8928DC" modified="2012-04-18" version="1">MIMQIKIERIYTKPADLDGYRILVDRVWPRGISKVDAHLDTWAKTIGPSTPLRKWFNHEDAKYPEFKQRYLNELATNPDLADFKQLVKTHLTTQNIILLYGAKNEQHNQAVVLKELLEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJH1</accession>
    <name>H4GJH1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_18568</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000039">
        <property type="protein sequence ID" value="EHS86614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122204.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000039.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18568"/>
    <dbReference type="EnsemblBacteria" id="EHS86614">
        <property type="protein sequence ID" value="EHS86614"/>
        <property type="gene ID" value="PS3_18568"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.922"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="108" mass="12340" checksum="602C23FF22C1AFB7" modified="2012-04-18" version="1">MGKKDQYKVLKLYGIPVDADPSGNYQLRTDANDQIKVHSWRIGKHTKGKYTGPGQLMLTENNLTVVILKAEPMDFKDRHQEVPMQRFLTIQVTDEVLARGLGLLKEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJK5</accession>
    <name>H4GJK5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglycerate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_8602</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122238.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8602"/>
    <dbReference type="EnsemblBacteria" id="EHS86515">
        <property type="protein sequence ID" value="EHS86515"/>
        <property type="gene ID" value="PS3_8602"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.979"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="221" mass="24575" checksum="CE6AB71074A06084" modified="2012-04-18" version="1">MAIDVYMVRHGQTYLNEFGKMQGWSDAPLTDQGWKDAKRAGEALSEIDFDFAFTSDLKRAIDTAQTILRNHPTKIQKATPNSAFRESFFGYFEGLHSGFTATTIGGALGVKTWEQLTEQVGIEKSRDLTKAADPFKRAEDNRDFWERLAPGFETLRSLPDQSKVLLVSHGMTIRSIISKYGQPGQALIAPQNGAITKLVITPTDIKVAFYNQLTIPQGDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJL5</accession>
    <name>H4GJL5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Xaa-Pro dipeptidyl-peptidase</fullName>
            <ecNumber evidence="5">3.4.14.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">X-Pro dipeptidyl-peptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">X-prolyl-dipeptidyl aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PS3_11337</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolyzes Xaa-Pro-|- bonds to release unblocked, N-terminal dipeptides from substrates including Ala-Pro-|-p-nitroanilide and (sequentially) Tyr-Pro-|-Phe-Pro-|-Gly-Pro-|-Ile.</text>
            <dbReference type="EC" id="3.4.14.11"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.11" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122248.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11337"/>
    <dbReference type="MEROPS" id="S15.001"/>
    <dbReference type="EnsemblBacteria" id="EHS86525">
        <property type="protein sequence ID" value="EHS86525"/>
        <property type="gene ID" value="PS3_11337"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.993"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008252">
        <property type="entry name" value="Pept_S15_Xpro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015251">
        <property type="entry name" value="PepX_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036313">
        <property type="entry name" value="PepX_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000383">
        <property type="entry name" value="Xaa-Pro-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013736">
        <property type="entry name" value="Xaa-Pro_dipept_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02129">
        <property type="entry name" value="Peptidase_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08530">
        <property type="entry name" value="PepX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09168">
        <property type="entry name" value="PepX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00923">
        <property type="entry name" value="LACTOPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00939">
        <property type="entry name" value="PepX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00940">
        <property type="entry name" value="PepX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81761">
        <property type="entry name" value="SSF81761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="PepX_N" evidence="14">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="PepX_C" evidence="13">
        <location>
            <begin position="538"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EHS86525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="784" mass="89148" checksum="A6315F9D95F01113" modified="2012-04-18" version="1">MKINQFANQLIPGCQPLTELAQLRLLTTDELETCQPKELWQRLLWRVDCSGTTDAQHQLHLHDYLATPSMALDEWLANDQPITDEIFARVALQLLDFEPALDFSLADPLADAQRMNLQLPTYELWNQAAVARGFYQLLLTRTKHGQTWLDYLTGQGLLTWTYQLPAKQKPLLVNGQPIACFDPHQFIYEVVYVEMDHDTDFDGQADLIKVEIMRPADSDHGLKVPAVFTASPYNQGTNDRWGEIITHDVNQPLTHLALDHQAPQETKFTNPQRCQAVTGHAQVATETFSNTPGYTLNNYLAPRGYAIVYAAGIGTKDSDGLQTCGSPEQTESMKVVVEWLHGIRHAYTDRTSGITIKASWCNGNVAMTGRSYLGTLSTAVATTGVPGLKAIISEAAISSWYDYYRENGLVMAPMGFQGEDADVLAAETFSRTKRPADYQRIAAINQKYLAQMTKAQDRASGQYNDFWRQRDYLPHVKQIKCDVMMVHGLNDWNVKPSNVKHLYDRLQSLPGDHKLILHQGEHIYINAFPSLDFSEMVNLWLAQKLWEVDNHADQLLPNLIVQDNVKAQTWTTYDHWNAPAHQVALGNETIQFSDRQPEAQYQQWCKDPNKWAKVLLSKPNRFSYSIPLTALSSNYYQGTPHLSLTVQSSLDHGMISAYLVDRGRERRLTISPTMVERQGIQLGYHWQTDDLRDFKLQSQPSDYKMISFGHANLQNPHDPEFAIELAAHQWVTVEFDLQPIFHHLANHHHLELVIFGTDYQMSLRGNENITYQVDLSKSSLTLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJM4</accession>
    <name>H4GJM4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative mevalonate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_5984</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000043">
        <property type="protein sequence ID" value="EHS86439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122257.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000043.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5984"/>
    <dbReference type="EnsemblBacteria" id="EHS86439">
        <property type="protein sequence ID" value="EHS86439"/>
        <property type="gene ID" value="PS3_5984"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1003"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="PTHR43290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="6">
        <location>
            <begin position="89"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP_kinases_C" evidence="7">
        <location>
            <begin position="220"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="314" mass="33586" checksum="6CCD565D9FFC97A2" modified="2012-04-18" version="1">MVNSATGTSHAKIILIGEHSVVYHQPAIALPLPSIQTIATLNTAPSRHQIISESFTGELDQLPVQMLGIQRLIEQLMERFADHQSGWVLKITSQIPQERGMGSSAAVAVAITRAFFNFYETPLERTELLELAKIEEVETHRNPSGLDAATVSSDQPLWFIKGESGQAFPLNLSGALVIADTGIKGATKAAIQAVKEKLETDPTAHERIKHLGELTKAAKQAVIDNNLEMLGQILDNAQLDLQTLKVSSSELNHLIQVANRAGALGAKLTGGGRGGCMIALAQNLSSARQIASQLMAHGAKQTNIQPFKGATTNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJQ6</accession>
    <name>H4GJQ6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nucleoside deoxyribosyltransferase (N-deoxyribosyltransferase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_1455</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000047">
        <property type="protein sequence ID" value="EHS86206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122289.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000047.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_1455"/>
    <dbReference type="EnsemblBacteria" id="EHS86206">
        <property type="protein sequence ID" value="EHS86206"/>
        <property type="gene ID" value="PS3_1455"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1059"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007710">
        <property type="entry name" value="Nucleoside_deoxyribTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05014">
        <property type="entry name" value="Nuc_deoxyrib_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="168" mass="18872" checksum="54282776716414EF" modified="2012-04-18" version="1">MKNTQAQPITKVYLATSFFNDQQKARVTKALEVLETNATVGVVHQPFDFQYKDALIDHDPAGVFGTLEWQVATYNNDVNAVGNSDVCVALYDMDQEDVGIAFEFGMFVALHKPIILLPFSQKPAEDYEVNLMLARGVSTWLQPNDFDQLADFNFNHPMAQPNVPYPVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJQ7</accession>
    <name>H4GJQ7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_3558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000047">
        <property type="protein sequence ID" value="EHS86207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122290.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000047.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3558"/>
    <dbReference type="EnsemblBacteria" id="EHS86207">
        <property type="protein sequence ID" value="EHS86207"/>
        <property type="gene ID" value="PS3_3558"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1060"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="28"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="218" mass="24590" checksum="87F1E0A91B012989" modified="2012-04-18" version="1">MLNNHQLDAIKAYAKAALNDDQTGHAMDHVMRVVNLATRLAEAEQVDATIPVMAAYLHDTIDPKLVADQDQAVTKLRTFLQTNQINPSDIDAVMDIITQMSFAKTLNGERPSLSIAGQIVQDADWLDAIGAIGIVRAIYYGARHQEKIYDSSIPPREHLDEQSYRNLADETIINHFYEKLLKIKDMLNTPAARQIATDRQIVMLNFLDDFKDEWDGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJR8</accession>
    <name>H4GJR8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Amino acid ABC superfamily ATP binding cassette transporter, binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_13234</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial solute-binding protein 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000047">
        <property type="protein sequence ID" value="EHS86218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13234"/>
    <dbReference type="EnsemblBacteria" id="EHS86218">
        <property type="protein sequence ID" value="EHS86218"/>
        <property type="gene ID" value="PS3_13234"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1076"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018313">
        <property type="entry name" value="SBP_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01039">
        <property type="entry name" value="SBP_BACTERIAL_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PBPb" evidence="4">
        <location>
            <begin position="14"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="265" mass="29139" checksum="1B07BF52B4BBE394" modified="2012-04-18" version="1">MTACGKKSAASKNTLIVATSGTLYPTSYHDSKTKKLTGYDVEVVRAIAKKLHKKVVFKEYSVDGMLTAVKTGKADMAVDDFAISPTRKKKFAMSIAFKHSFNSIIVRSSDNSGINSWADLKDKKAGGEAGTNYQRLAQQLGAKLVNYDNVSNDVYLRDLQDGKTDFIMNDYYLQKLALAANPNSGLKIMPNMYFTTNEDGSGEGIIMSKDDPKLTKEVNSALKALLKDGTIKKLAMKFYGADVTKQPSEHISKHFTISSKYTGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJT2</accession>
    <name>H4GJT2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fumarase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_9910</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122315.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9910"/>
    <dbReference type="EnsemblBacteria" id="EHS86119">
        <property type="protein sequence ID" value="EHS86119"/>
        <property type="gene ID" value="PS3_9910"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1109"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018951">
        <property type="entry name" value="Fumarase_C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10415">
        <property type="entry name" value="FumaraseC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lyase_1" evidence="2">
        <location>
            <begin position="9"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="FumaraseC_C" evidence="3">
        <location>
            <begin position="408"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="459" mass="50581" checksum="24ECFB7E9362F95B" modified="2012-04-18" version="1">MRIEHDSLGELAVPKDALYGIHTLRARENFPLESIKNDPELCKNFLLVKEAAAIANMKAGAWEDKRIPTAIISSCDYLYHNFSYYHQEFDLPALQGGAGTSTNMNINEIIANKALQELGLPLGDYQYISPNDDVNKSQSTNDTYPTAGHLTMIKFTRTVMDELSKVITTLQDLATKYQDVLKMGRTQLEDAVPTTFGRSFKAYASVLSRDMDRLAAVCQELMTIPLGGTAIGTGVTGNRSYMDTVVDELATLTKLPLKQCDDLVDGIQNNDCYVSLSNAYRSLAVDLSKMSNDLRLLNSGPQDGLSEINLPKRQAGSSIMPGKVNPVIPEFCTQVAYQVMGHATTVAMAAEAGQLELNAFEPVAFYDLFEDARLLRKALHTLDKNCLQGITINIERCIAMVEHSAETATALSPVLGYERTTEIIKESLRTGKSIRALVKDEISQEKLDELLSPASFFVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJU3</accession>
    <name>H4GJU3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">ATP-dependent DNA helicase RecG</fullName>
            <ecNumber evidence="3 7">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">recG</name>
        <name evidence="10" type="ORF">PS3_13782</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the helicase family. RecG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3 7"/>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122326.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13782"/>
    <dbReference type="EnsemblBacteria" id="EHS86130">
        <property type="protein sequence ID" value="EHS86130"/>
        <property type="gene ID" value="PS3_13782"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1121"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004609">
        <property type="entry name" value="ATP-dep_DNA_helicase_RecG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045562">
        <property type="entry name" value="RecG_dom3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033454">
        <property type="entry name" value="RecG_wedge"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19833">
        <property type="entry name" value="RecG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17191">
        <property type="entry name" value="RecG_wedge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00643">
        <property type="entry name" value="recG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="269"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="449"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS86130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="678" mass="76470" checksum="6CD82CFDFFF494F9" modified="2012-04-18" version="1">MLSLQDSVGKLKGVGPKMGENLAILDILTVEDLLTYFPARYDEFTPINLEDAKDKQRITVKGTVISEPMMSRFGYRRSRLSFRIMVGHAVAQVTFFNQPYLAKSVQMNTDVTVMGTWDLSRNEIKGTKLLTSNLNEEKGMGAIYPANKHIKQATIRKLIRQAYDQYQRVIATLLPISLRQRYQLMERREMIKQLHFPDNREMAKLAQRTAAYEEFFLFQLRLQALRRAHRQENGIQILYDNEELREFIRTIPFELTSAQKRVVNEICRDLRSPYQMNRLLQGDVGSGKTIVAAIAIYAAISTGYQVAMMAPTEILAEQHAEKLARTFSGTHVNVALLTGSMKAKQHQELLNQIENGSVNLIIGTHALIQEKVDYANLGLAIIDEQHRFGVKQRQALREKGDHPDILAMTATPIPRTLAITAYGEMDVSIIDELPAGRQPIKTTWLREKQLDQAIDFLKGQLEQGAQAYIVSPLIEESESLDVQNATALAEQYQQRFAPHYQVGLLHGRMTNEEKSAVMQDFQDGKLQVLVSTTVIEVGVDNPNATVMMIYNADRFGLAQLHQLRGRVGRGSRQSYCLLVADPKTDEGTARMETMVATTDGFVVAQRDLELRGSGDVLGSRQSGVPEFKVGDPVADLKMLQIARSDVGNLLNEPSWDEKAENQPLVLYLKRHQLATHFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJV0</accession>
    <name>H4GJV0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Serine/threonine specific protein phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_18743</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122333.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18743"/>
    <dbReference type="EnsemblBacteria" id="EHS86137">
        <property type="protein sequence ID" value="EHS86137"/>
        <property type="gene ID" value="PS3_18743"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1128"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006470">
        <property type="term" value="P:protein dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PTHR13832"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="247" mass="26822" checksum="B8BC90ED0CD1BD82" modified="2012-04-18" version="1">MQTAFLTDVGLKRIDNQDRVAITTAPSGSQLLIIADGIGGNQGGSKAATMTVDFLGNTFLSDTPATPDAAKRWFYQYVQTINDQILETANQDPQYSGMGTTLVAAIVLAPTVIVAHIGDSRAYLLHDDTLNQLTRDHTLVNELVRIGELTPTQAKQSPHQNVITRAVGVAEVAELEVNAYVLGQGDVFMLCSDGLYKEVTNNQMQRIIKMKRISVQEKCHRLIGRALKTGGADNISVLICQNTPKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJV3</accession>
    <name>H4GJV3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Primosomal protein N'</fullName>
            <ecNumber evidence="9">3.6.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP-dependent helicase PriA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">priA</name>
        <name evidence="12" type="ORF">PS3_4115</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the primosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the helicase family. PriA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122336.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4115"/>
    <dbReference type="EnsemblBacteria" id="EHS86140">
        <property type="protein sequence ID" value="EHS86140"/>
        <property type="gene ID" value="PS3_4115"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1131"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006268">
        <property type="term" value="P:DNA unwinding involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00983">
        <property type="entry name" value="PriA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005259">
        <property type="entry name" value="PriA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041222">
        <property type="entry name" value="PriA_3primeBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042115">
        <property type="entry name" value="PriA_3primeBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041236">
        <property type="entry name" value="PriA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040498">
        <property type="entry name" value="PriA_CRR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17764">
        <property type="entry name" value="PriA_3primeBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18074">
        <property type="entry name" value="PriA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18319">
        <property type="entry name" value="PriA_CRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00595">
        <property type="entry name" value="priA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 9" id="KW-0639">Primosome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="283"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="546"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="9">
        <location>
            <begin position="511"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="9">
        <location>
            <begin position="538"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS86140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="805" mass="91518" checksum="B32ACAAC10DA5EDA" modified="2012-04-18" version="1">MADLAQVVVDVPTMQTNQPYTYLIPDRLVEIAQAGMRVIVPFGAGHREVQGFIVGKSSQTEYDGKLKPISAVMDLNPVVNSELLHLSHWMAETTYAFWITCLYTMLPNLLKAKSQRMVRIIDELSESDQMALFGNQDELDFSKVQHDPETVQRLSQLQRDKKIEFVYQVNDRARVKTVTAIKASLSFEQYEDELQGITKNAKAQHQLILYLQTLGDQVVALKEAQATTGLSSATFTLGQSRGWLTKVQQEVYRDPFLQVSVPQKQILTLNNEQRVAVDNIDQAIEQSVSQTFLLQGVTGSGKTEVYLRVMQMALDQGKTAMMLVPEISLTPQIVGRVRSWFGDQVAVLHSGLSNGERYDEWRRITRGEVKVVVGTRSAIFAPLENLGVIIMDEEHEGSYKQDESPRYHAREVAKWRSDFHHAPLVLGSATPSLESRSRAEAGLYHRLVLSKRANRQALPPIEIVDMRPEIQKKGETNFSQQLLSALHARLERHEQSVLLLNRRGFSSFVMCRDCGYVLKCPNCDISLTLHMDSHSMKCHYCGHEEAIPNRCPQCQSRRISYYGTGTEKVAAELGELLPNARIIRMDVDTTRRKGMHQKLLDRFGNHEADILLGTQMIAKGLDYPNVTLVGVLNADTGIDLPDFRAAERSFDLLTQVSGRAGRANKAGQVIIQTFNPDNYVIQLVKEHDYERFFATEMNLRKLAAYPPYYYTVRIMGSHPDEKKVAKAMFEIRRQLDQVTDSGTIFLGPTPRAIARLKNRYYYQIIIKYRQDQKLHSLLSQIMHEAQSKYSRDLQISIDPDPQYFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJV9</accession>
    <name>H4GJV9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_5996</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000050">
        <property type="protein sequence ID" value="EHS86033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122342.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000050.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5996"/>
    <dbReference type="EnsemblBacteria" id="EHS86033">
        <property type="protein sequence ID" value="EHS86033"/>
        <property type="gene ID" value="PS3_5996"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1141"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="PTHR43029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="348"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium_transp" evidence="9">
        <location>
            <begin position="8"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS86033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="428" mass="46679" checksum="CA39F00E03377564" modified="2012-04-18" version="1">MSAVNTSFLILSSILVLFMTPGLAFFYGGLVSRRNVVNTMLSVFFICGLAIVLWFACGYSLAFSGDLGGVIGNLKAVFFHGVALNHVMDTGITTGVYSLFQMMFAIITPALFIGAVVGRINFRFLVWFIIIWSLFIYYPMVHLVWSADGWLAKLGTLDFAGGTVVHINAGVTALMLSIWLGPRTKRESQHYNLPWVLLGTTILWIGWYGFNAGSALAVNSVALQAVFTTTIAAATSMVAWMIIEVYLKKRVTLVGTCTGTLCGLVGITPAAGYVTLAGAFWIGMIATIVSYVFVEYAKPKLKFDDPLDAFGCHGMSGIWGSIATGLFATHHVNSAVTTDGLFYGGGSHLLMVQIIATIATMVFVCLGVSIVTWVLKQFIPMRVTKKDEYLGLDIAEHGEMVDDNQGPRRDISQHPDEFRGQLSWLKRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJW2</accession>
    <name>H4GJW2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(P)H-hydrate epimerase</fullName>
            <ecNumber evidence="2">5.1.99.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">NAD(P)HX epimerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nnrE</name>
        <name evidence="4" type="ORF">PS3_13410</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="2">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.99.6" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000050">
        <property type="protein sequence ID" value="EHS86036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122345.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000050.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13410"/>
    <dbReference type="EnsemblBacteria" id="EHS86036">
        <property type="protein sequence ID" value="EHS86036"/>
        <property type="gene ID" value="PS3_13410"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1145"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NADHX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052857">
        <property type="term" value="F:NADPHX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01966">
        <property type="entry name" value="NADHX_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="SSF64153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00197">
        <property type="entry name" value="yjeF_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF N-terminal" evidence="3">
        <location>
            <begin position="10"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="NADPHX" evidence="2">
        <location>
            <begin position="59"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="NADPHX" evidence="2">
        <location>
            <begin position="126"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="208" mass="22219" checksum="5C5907E6364FFEC9" modified="2012-04-18" version="1">MTKTVTAQEMQMADQFTINTIGIPGMVLMERAALALRDIIQVQFTHQLSQVVVVAGSGNNGGDGLALARLLHLQKVPIEILNVGNPDHASQQHQDQDHICQYYQIPQTRDIKILNQASLIIDAIFGVGLDRPITGNYLTVINAINQATAPVLAVDVPSGMNTDTGEIMGAAVQANMTVTMAFNKQGLTTASGQQLAGEVVIADDIGIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJW3</accession>
    <name>H4GJW3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">D-lactate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_9711</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000050">
        <property type="protein sequence ID" value="EHS86037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122346.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000050.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9711"/>
    <dbReference type="EnsemblBacteria" id="EHS86037">
        <property type="protein sequence ID" value="EHS86037"/>
        <property type="gene ID" value="PS3_9711"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1146"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="4">
        <location>
            <begin position="9"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="5">
        <location>
            <begin position="111"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS86037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="329" mass="36785" checksum="E89E31B8FE7023F6" modified="2012-04-18" version="1">MKIIMYDTHQHERPYAQIWAQAHHQELTMVEEPLTMETVHLAEGYDGLSVQQTIQIDNDIYPMLANYGINHISTRTAGFDNFDLAVAKQHGITITNIPSYSPRAIAEMGLTQAMYLLRNIGRFHEQMDQNHDFTWSGNLISNEIYRCTVGLIGTGRIGTATAQIYKALGARVLAYDPRYHPELEPFVEFVDRDTILRQADIITLHTPLTTQTKNMIGAAELRVMKPTAILINVARGGLVDTQALIKALQDHEIAGAGLDTLANEQNLFGHQVSPDQIPEDYKILRAMPNVIITPHVAFLTQTAVQNLVQISLDDTVKLAQGQRTNHAVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJY3</accession>
    <name>H4GJY3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">3-hydroxyisobutyrate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_19589</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS86000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122366.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19589"/>
    <dbReference type="EnsemblBacteria" id="EHS86000">
        <property type="protein sequence ID" value="EHS86000"/>
        <property type="gene ID" value="PS3_19589"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1179"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016054">
        <property type="term" value="P:organic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="NADP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_2" evidence="2">
        <location>
            <begin position="3"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="NAD_binding_11" evidence="3">
        <location>
            <begin position="165"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000103-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="286" mass="30842" checksum="0E7C2B0FEAACC9C7" modified="2012-04-18" version="1">MTKIGFIGTGLMGHGMIRNLLKNGYQVQIWNRTKTKAEDLINDGANWTDDAAQCAHDVDFIITIVGYPKDVEEVYFGEHGVLAGANPGTTIIDMTTTSPQLAQKIYQAAVDHKLLALDAPVSGGLAGAENGSLSIMVGGDQATFEHAFPVFTAMGKQIVLQGPAGAGQHTKMANQIALAGALAGVCESLRYAKAVGLDPQKMFDTIRAGAAGSAQMEAFMPMLINNDFKATFYMKHFIKDLKIAHNEANDRDLKLAILDQILKECQAVESMGFGDEGTQALYHWYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJY9</accession>
    <name>H4GJY9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_12357</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS86006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122372.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12357"/>
    <dbReference type="EnsemblBacteria" id="EHS86006">
        <property type="protein sequence ID" value="EHS86006"/>
        <property type="gene ID" value="PS3_12357"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1195"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="2">
        <location>
            <begin position="48"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="399" mass="44091" checksum="AEF0B42653557810" modified="2012-04-18" version="1">MATFKYSKRVPADGTDAVGAILAAAADPSIISFAGGLPAPELFPVEAMKKAAEAAFDEHGQQVMQYGDARGVAAMRELVTKIVKEHENIDAKDENICMTSGSEQILELAAKALVNPGDTVLVEDPTYLCAVDAFKSYGANLVGVPTDEDGLIPAELEKTVQAHPEAKFLYVIPTFQNPTGRTMPAERRKQVAELASKYDFLVFEDNPYGDIRFEGEHVDAIKSYDKTDNVVYMGTFSKILSPGFRLGYFVADPGFVKKFTVLKQTADLGTDNLVQWCTVKFYEQNDVNEHVKKISDLYGQRKKLMEEGMQKYFPEGVTYTHPEGGMFLWVTVPGVEDTTELFETCLKQKVAFVPGEPFYAAKPEPGHLRLNYSNMKPDQIEEGLKRLGKALEEAIAANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK25</accession>
    <name>H4GK25_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoribosylformylglycinamidine synthase subunit PurL</fullName>
            <shortName evidence="7">FGAM synthase</shortName>
            <ecNumber evidence="7">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Formylglycinamide ribonucleotide amidotransferase subunit II</fullName>
            <shortName evidence="7">FGAR amidotransferase II</shortName>
            <shortName evidence="7">FGAR-AT II</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Glutamine amidotransferase PurL</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphoribosylformylglycinamidine synthase subunit II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">purL</name>
        <name evidence="11" type="ORF">PS3_11896</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the FGAMS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000055">
        <property type="protein sequence ID" value="EHS85841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122408.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000055.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11896"/>
    <dbReference type="EnsemblBacteria" id="EHS85841">
        <property type="protein sequence ID" value="EHS85841"/>
        <property type="gene ID" value="PS3_11896"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1257"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00420">
        <property type="entry name" value="PurL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010074">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555">
        <property type="entry name" value="PTHR43555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001587">
        <property type="entry name" value="FGAM_synthase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01736">
        <property type="entry name" value="FGAM_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FGAR-AT_linker" evidence="10">
        <location>
            <begin position="10"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS" evidence="8">
        <location>
            <begin position="90"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="9">
        <location>
            <begin position="206"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS" evidence="8">
        <location>
            <begin position="459"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="9">
        <location>
            <begin position="576"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="98"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="318"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="501"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="538"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="539"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="541"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS85841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="742" mass="80197" checksum="AFEA3F876ED1E78B" modified="2012-04-18" version="1">MQQAMTPEEIKQQKPYLDWSLTEHEYDYICDHLLHRLPNFTETGLFSAMWSEHCSYKKSKPVLRQFPNKNDRVLQGPGEGAGVVDIDDGQAVVFKAESHNHPSTVEPYQGAATGVGGILRDIFSMGARPIASLDSLHFGELDQVSNQMKLTGVVKGIGDYGNCMGIPTVAGETTFDPCYSGNVLVNAMSVGLMDQKDIQAGVAAGVGNAVMYVGAKTGRDGIHGATFASAEFSDENATQRSAVQVGDPFMEKLLMEACLELIQQHADWLIGIQDMGAAGIVSSSAEMVSEGESGVELNLDLVPQREPNMSAYEIMLSESQERMLLCVKQGHEEDVKQIFDFYDLEAVVIGRITEGHDYVLYHDGEKVCDIPVSTLTDDVLEEPSEEAKPQRIAAAEQAPAWQPVIDDPAATLKDLLGQTTIADKSSLYQQYDSQVRTSTVVGPGSDARVIRVRGTKKGLAMTTDGNGRFVYLSPKVGGQIALVEAAANVIASGAQPLAITDCLNYGNPNDPEIFWELHQSTAGMAEACRVFDTPVISGNVSLYNENNGKAIHPTPMVGMVGLIKDLDHVIPMQAQQAGDLVYLLGQTGDDYAGSELQKMLQGDIDGVLNDFDLKESHQMMMNLLAAMQAGHVASAHDLSEGGLGVALSEALFDTDLGFELNLTHLTNAQLFSETPGRFIVTVSPDQQDAFEDALGDDAQLVGEVTNTHWLDTRTADGDMNVSVADLQTTWKEAIPCLLKSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK31</accession>
    <name>H4GK31_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">N5-carboxyaminoimidazole ribonucleotide mutase</fullName>
            <shortName evidence="3 4">N5-CAIR mutase</shortName>
            <ecNumber evidence="3 4">5.4.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5-(carboxyamino)imidazole ribonucleotide mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purE</name>
        <name evidence="7" type="ORF">PS3_3367</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="EC" id="5.4.99.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.18" evidence="3 4"/>
    <dbReference type="EMBL" id="AICN01000055">
        <property type="protein sequence ID" value="EHS85847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122414.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000055.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3367"/>
    <dbReference type="EnsemblBacteria" id="EHS85847">
        <property type="protein sequence ID" value="EHS85847"/>
        <property type="gene ID" value="PS3_3367"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1263"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034023">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024694">
        <property type="entry name" value="PurE_prokaryotes"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001338">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 3 4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRC" evidence="6">
        <location>
            <begin position="2"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001338-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="161" mass="16527" checksum="9841D2D3454CAD2F" modified="2012-04-18" version="1">MSQISVVMGSTSDWPTVKLACDILDEFGVDYDKHVVSAHRMPDEMFAFAKSAVANGTKVIIACAGGAAHLPGMVAANTPLPVIGIPAKTSALGGMDSLLSIVQMPAGIPVATTAIGAAGAKNAALLALQILGTSDPAIQNQIVEYRQKMHDQAVESEADLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK42</accession>
    <name>H4GK42_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA_28 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_21981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000056">
        <property type="protein sequence ID" value="EHS85809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122425.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000056.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21981"/>
    <dbReference type="EnsemblBacteria" id="EHS85809">
        <property type="protein sequence ID" value="EHS85809"/>
        <property type="gene ID" value="PS3_21981"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1278"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038727">
        <property type="entry name" value="NadR/Ttd14_AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13521">
        <property type="entry name" value="AAA_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_28" evidence="1">
        <location>
            <begin position="9"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="259" mass="29507" checksum="42EE966A280454A7" modified="2012-04-18" version="1">MNNNEHIRLVISGTYSTGKTTTTTALSILTGIPLINALSAREILTDLYPGRRFQDMTADELMALGLKRFEERVRAETIAYKEAGAFISDGSVLNEWIYGTVRMKVGINPGSTLPHRIAKAVMGVPFKPFMKKYLDSYGTVVRQHAKEWYTDVVHLPVEFPMDPDGHRPVSEEYRNLSDIEIQQRFKELGLPPFMVSGQVAERLQTIVDQYQLPQIMQIEEAIELANQRIATNREAVSKKMIEQYHEPTLSEKVKLMTKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKB9</accession>
    <name>H4GKB9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta-galactosidase</fullName>
            <ecNumber evidence="2">3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_22294</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122502.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_22294"/>
    <dbReference type="EnsemblBacteria" id="EHS85571">
        <property type="protein sequence ID" value="EHS85571"/>
        <property type="gene ID" value="PS3_22294"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1390"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009341">
        <property type="term" value="C:beta-galactosidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004199">
        <property type="entry name" value="B-gal_small/dom_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02929">
        <property type="entry name" value="Bgal_small_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01038">
        <property type="entry name" value="Bgal_small_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bgal_small_N" evidence="5">
        <location>
            <begin position="17"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS85571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="318" mass="35432" checksum="67C20EE2389B970F" modified="2012-04-18" version="1">MDYTNQMQIIYDDGMLGLAGPRFHYIFGYERAGLESLVVDGKEWLYRVPQPTFWRATTDNDRGNQFNIKSAQWLAADYVVPCVDIDLTVDDHHYDQLPIALVTNQFSNQTLAEQVEIKYTYQTVTVPQTTVTVTYLVVADGTITVRAHYQGKANLPELPVFGLRLIMPTPTTGYTYQGLSGETYPDRMAGATKGQFEVAGMPVTPYLVPQECGMHMATDWVTVTRATTLNNADPDLSPFSLKFSALDKPFAFSCLPYTSSELENATHQEELPPIHRTVMTIAGAVRGVGGIDSWGADVEPQYHLDASQDHEVSFQITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKC6</accession>
    <name>H4GKC6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SprT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_20603</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122509.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20603"/>
    <dbReference type="EnsemblBacteria" id="EHS85578">
        <property type="protein sequence ID" value="EHS85578"/>
        <property type="gene ID" value="PS3_20603"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1397"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051716">
        <property type="term" value="P:cellular response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006640">
        <property type="entry name" value="SprT-like_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10263">
        <property type="entry name" value="SprT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00731">
        <property type="entry name" value="SprT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SprT-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="147" mass="17621" checksum="95B1950F9E71E1EA" modified="2012-04-18" version="1">MTNQELQAHTEEWSRTLFKRPFKHQIFFNRRLKTTGGRYHLNDHHIDINPLMYECFDLANLRAVVVHELCHYHLHLMGRDYHHRSSDFRHLLALTGGQRYAPAIPNREAKYSYQCQTCGVIVNRQRRFNPRLYVCRQCGGRFKLIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKH1</accession>
    <name>H4GKH1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flavodoxin_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_19792</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000067">
        <property type="protein sequence ID" value="EHS85385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19792"/>
    <dbReference type="EnsemblBacteria" id="EHS85385">
        <property type="protein sequence ID" value="EHS85385"/>
        <property type="gene ID" value="PS3_19792"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1500"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003680">
        <property type="entry name" value="Flavodoxin_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02525">
        <property type="entry name" value="Flavodoxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin_2" evidence="1">
        <location>
            <begin position="33"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="155" mass="17568" checksum="C479C4739DF5328B" modified="2012-04-18" version="1">MKGATEAGNELYRFDAGRRVNEFSLIQLVDNHSGMEVEQEPDDVIKEEVMPKLLAADLVAFGSSLYYYGINSALKAVIDRFYSYNHELHGDKEAITLISGYGDEDAFKSLELYFDQLLQYMRWPLVGKALAADSWNEAKLAQHVEAAYQLGRKIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKJ6</accession>
    <name>H4GKJ6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Teichoic acid glycosylation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_9728</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GtrA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122578.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9728"/>
    <dbReference type="EnsemblBacteria" id="EHS85276">
        <property type="protein sequence ID" value="EHS85276"/>
        <property type="gene ID" value="PS3_9728"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1540"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007267">
        <property type="entry name" value="GtrA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04138">
        <property type="entry name" value="GtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="GtrA" evidence="7">
        <location>
            <begin position="15"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="145" mass="16885" checksum="2C163F441AF99F7B" modified="2012-04-18" version="1">MLMELFNKYKSIIAYLFWGVVTTIINIASYQLMIMYTHLGVTAATSIAWFLSVFVAYLTNKVWVFGSHYTTVSDFLVECLRFFFYRALTLIIDLAFMNIGVHVFGFDSDLQQFILKVIDNVIVVIANYIFSKWLIFKDNRDIADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKM7</accession>
    <name>H4GKM7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Enolase</fullName>
            <ecNumber evidence="10">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">eno</name>
        <name evidence="15" type="ORF">PS3_11028</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="10">The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Secreted</location>
        </subcellularLocation>
        <text evidence="10">Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the enolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="10"/>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122609.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11028"/>
    <dbReference type="EnsemblBacteria" id="EHS85196">
        <property type="protein sequence ID" value="EHS85196"/>
        <property type="gene ID" value="PS3_11028"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1585"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="PTHR11902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Enolase_N" evidence="14">
        <location>
            <begin position="4"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase_C" evidence="13">
        <location>
            <begin position="139"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10 12">
        <location>
            <begin position="367"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10 11">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10 11">
        <location>
            <position position="340"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <position position="315"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent; in inhibited form" evidence="10">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EHS85196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="440" mass="47708" checksum="6890B85080F196A8" modified="2012-04-18" version="1">MSLITDIYAREVLDSRGNPTVEAEVYTEAGGAGRGIVPSGASTGEHEAVELRDGDKDRFGGKGVLKAVANVNNVIAKELVGMEVTDQVAIDKAMIALDGTPNKGKLGANAILAVSLAAARAAADELEVPLYNYLGGFNAHLMPTPMMNVINGGAHSDNKVDFQEFMIMPVGAKSVREAIRMGSETFHALKKALEADGKATAVGDEGGFAPDFANNEEPFEYLIKAIEAAGYKPGKDVAIAADVAASELWNDEDKKYKLRWSTGEEYTTEEWIEYLTGLIEKYPIVSVEDPIDENNWDDWVTITDKLGKKVQLVGDDFFVTNTEYLAKGIKMGAANSILIKLNQIGTLTETVEAIEMAKEAGYTAIVSHRSGETEDTTIADLVVALNAGQIKTGSMSRTDRLAKYNQLMRIEDNLGDVAQYKGINSFYNLSEQTREEIENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKN5</accession>
    <name>H4GKN5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cytochrome D ubiquinol oxidase subunit I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PS3_8995</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome ubiquinol oxidase subunit 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122617.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8995"/>
    <dbReference type="EnsemblBacteria" id="EHS84955">
        <property type="protein sequence ID" value="EHS84955"/>
        <property type="gene ID" value="PS3_8995"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1595"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070069">
        <property type="term" value="C:cytochrome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002585">
        <property type="entry name" value="Cyt-d_ubiquinol_oxidase_su_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30365">
        <property type="entry name" value="PTHR30365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01654">
        <property type="entry name" value="Cyt_bd_oxida_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006446">
        <property type="entry name" value="Cyt_quinol_oxidase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 12" id="KW-0349">Heme</keyword>
    <keyword evidence="10 12" id="KW-0408">Iron</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="431"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR006446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS84955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="477" mass="53895" checksum="325BACDB7B655DA9" modified="2012-04-18" version="1">MSIVTLARFQFAMTTVFHFFFVPFSIGTAFAIAIMESMYVHTKNETYKKMARFWGNIFLLSFAVGVVTGLIQEFQFGMNWSDYSRFMGDIFGAPLAFEALVAFFMESTFIGLWTFTWDRVKPGLHSLFIWLTAIGSMISALWILTANSFMQHPVGYSIKNGRAVMTDFGALLKNPQLWFEYGHVVFAALMMGGTIVAGLTAFQLLKKQSLSSEVKDLYKKSMRLGLTLMLIFGALTVAWGDMQMKYLVKEQPMKFAAAEGVVTTTGKHAPWTIVGVYDTKTHESKGNLDIPYVLSILTYDKTSGSVKGMKEINAQLEKKYGTKIDNQKISYYPPVNTVFYSFRIMAAVGGWMMLVSIVGLFLSRKKAETLYKHKWTLWVVALTTFVPFIGNTAGWFVTEFGRFPWTVYGLFTIQQSVSPNVSVASLLTSNIVYFLLFTSLAIVMIYLVVRQLRKDPMSMDQPQFAEKILDPFDKGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKQ9</accession>
    <name>H4GKQ9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA polymerase III PolC-type</fullName>
            <shortName evidence="14">PolIII</shortName>
            <ecNumber evidence="14">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">polC</name>
        <name evidence="17" type="ORF">PS3_12180</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase.</text>
    </comment>
    <comment type="function">
        <text evidence="1 14">Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA polymerase type-C family. DnaE subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the DNA polymerase type-C family. PolC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="14"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122641.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12180"/>
    <dbReference type="EnsemblBacteria" id="EHS84979">
        <property type="protein sequence ID" value="EHS84979"/>
        <property type="gene ID" value="PS3_12180"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1632"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00356">
        <property type="entry name" value="DNApol_PolC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011708">
        <property type="entry name" value="DNA_pol3_alpha_NTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040982">
        <property type="entry name" value="DNA_pol3_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024754">
        <property type="entry name" value="DNA_PolC-like_N_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028112">
        <property type="entry name" value="DNA_PolC-type_N_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029460">
        <property type="entry name" value="DNAPol_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006054">
        <property type="entry name" value="DnaQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Exonuclease_RNaseT/DNA_pol3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006308">
        <property type="entry name" value="PolC_gram_pos"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044923">
        <property type="entry name" value="PolC_middle_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14480">
        <property type="entry name" value="DNA_pol3_a_NI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11490">
        <property type="entry name" value="DNA_pol3_a_NII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07733">
        <property type="entry name" value="DNA_pol3_alpha"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17657">
        <property type="entry name" value="DNA_pol3_finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14579">
        <property type="entry name" value="HHH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00573">
        <property type="entry name" value="dnaq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01405">
        <property type="entry name" value="polC_Gram_pos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14" id="KW-0235">DNA replication</keyword>
    <keyword evidence="11 14" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="10 14" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 14" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="POLIIIAc" evidence="16">
        <location>
            <begin position="333"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Exonuclease" evidence="15">
        <location>
            <begin position="423"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EHS84979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="1444" mass="162752" checksum="6E715EEADBB72438" modified="2012-04-18" version="1">MALSSKEQFNKLLQQINFTDPSLSNGELTQVTVHQKSRVWELYIHLDQVLPYPVFQQFYQAMVMSFQHLAQANVKIHIATETTSLDNQLVGAYWSGVIKMVAGDTGMARELSQTKEPQVVDNQVQLTLENAKVAEFVQRRLLGLIETGYQEVGFPAFRIKTFVDEDASVAKREAIQAKQEAAEQKLMAQAMQQIQQHKEQSQALEPVDGPVVIGKEINPKFDLQRMRTIVEEERSVVVEGYVFAKEIRELRSGRQLMVIEITDYTSSFAVKKFSRDKNDEAQFNTLKEGMWVKVRGSVQEDTYMNDLTINAYDINQVTHQERQDTAPEGEKRVELHLHSTMSTMDATNSVGDYVKQAIKWGHPAIAITDHAGVQAYPEAFNTAYDGKKKQDKIKMLYGVEANVVDDGVPLVYNEDHTALAGHVYVIFDVETTGLSAIYDRLIQVSAVKMQDGEVLDRFDEFIDPGFPLSAQTTELTSITNEMVRGSKTEAEVLQLFRDFCGDAIITGHNVSFDLGFMNTAYDRAHMSIISAPVIDTLPLARFLYPDLRGYRLNTLSKKFKIPLEHHHLASFDSEATGHLLHIFLKEAEERYQIQFVDDLNKHMADNNAYRHARPFHVSIIAQTQAGLKNLYKLISMSNVDYFYRVPRIPRSVLNQYREGLLLGSACANGEVFTAMMQKGYAEAKQKARYYDYLEVQPKPNYMPLIEQQMVADEGHLEDIIKNMVKLGQELDKPVVATGDAHYLNPEDAIYRKILISSQGGANQLNRTQRPEVPFRTTDEMLALFDFLGEDTAHQIVVTNTQAVAKMVDDGIHPVQDKLYPPNIPGANDELYNRTWETAKKWYGENMPDFVKERIKIELDSIIGNGFAVIYLIAQRLVAKSNKDGYLVGSRGSVGSSVVATFTGITEVNPLPPHYRCPECQHSEFFTKGEYSSGFDLPDKNCPECGTLMIGDGHDIPFQTFLGFSGNKVPDIDLNFSGDYQPIAHNYMKVLFGENNVYRAGTIGTVADKTAYGYVKGYERDHEIQFRKAEEDRLAKGATGVKRTTGQHPAGILIVPDDKEIYDFTPVQYPADDQSAAWETTHFDFHSIHDNILKMDILGHDDPTMIRALQDLSGIDPQTIPMDDPGVMSLFSSPEQLGVTEEQINSKTGTLGIPEFGTRFVRGMLEDTHPSNYSELLQISGLSHGTGVWLENAQELIQKGTATIANVIGCRDNIMTDLIHYGLDSETSFQVMEHVRKGKGIPDEWQEKMLEVGVPQWYLDSCLKIKYMFPRAHAAAYVLMALRIAYFKTYFPLVYYAAFFSVRADDFDVVAMSRGKEAVKAAMKEINDKGNDASAKEKGLLTILELANEMLERGFKFKMVDLERSDATDWLMDGDDTLIAPFRAIPGLGINVAKQIVAAREEKEFLSKQDLKERGKVSNTLIEFMTENHVLDALPDENQLSLFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKT7</accession>
    <name>H4GKT7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="3 15">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PS3_11020</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>(R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + NAD(+) = (R)-N(6)-lipoyl-L-lysyl-[protein] + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 15">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="13 15">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="15">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="3 15"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122669.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11020"/>
    <dbReference type="EnsemblBacteria" id="EHS85007">
        <property type="protein sequence ID" value="EHS85007"/>
        <property type="gene ID" value="PS3_11020"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1665"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006258">
        <property type="entry name" value="Lipoamide_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01350">
        <property type="entry name" value="lipoamide_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 13 15" id="KW-0274">FAD</keyword>
    <keyword evidence="5 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9 13 15" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11 15" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="17">
        <location>
            <begin position="11"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="16">
        <location>
            <begin position="349"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="447"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="148"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="184"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="14">
        <location>
            <begin position="48"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EHS85007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="468" mass="49385" checksum="CDAC3BC937B547AB" modified="2012-04-18" version="1">MVVGDYAIDLDTIVVGSGPGGYVAAVHAAELGQKVAVVERYDALGGVCLNVGCIPSKAMIQVSHEYLTAKDGADRGIETGDVKLDFDKVQEYRKQVVNRMSNGVAYLFKKNKIDVIHGTAFMKSDHSLRVINDDSAQTYTFKNLILATGSHPIEIPGFKFGGRVIDSTGLLALPALPKELVVIGGGYIGSELAAAYANFGSHVTILEGTDTILGAYEKDMVKIIEKRFKAKGIDIITGAMAKEAKDNGDSVEVSYELNGEVKTVTADYVAVAVGRKPNTDDLGLNIAGVETTDRGLVKVDAQGRTNVEHIYAIGDIVAGAALAHKASYEGKVAAEAIAGQSSVVDYRAMPAVCYVDPEIATTGLSADQAKAEGLDVKTAKFPFSANGRAVSMHSDEGFVRLVFTKDNEQVVGAQIIGSNASDMISELTLAIESGNTVEDIALTIHPHPTLSEAVMDAADVALGYPTNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKU2</accession>
    <name>H4GKU2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Azaleucine resistance protein AzlC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_5723</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AzlC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5723"/>
    <dbReference type="EnsemblBacteria" id="EHS85012">
        <property type="protein sequence ID" value="EHS85012"/>
        <property type="gene ID" value="PS3_5723"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1670"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011606">
        <property type="entry name" value="Brnchd-chn_aa_trnsp_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34979">
        <property type="entry name" value="PTHR34979"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03591">
        <property type="entry name" value="AzlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS85012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="229" mass="26179" checksum="9CF20DB50DEDD56D" modified="2012-04-18" version="1">MEWKYAFKKSFPVMIGYVILAVSYGLYMHNLHFDWWFPLLMAATIYGGSVEFLIANMLLLKFAPVTVLLITLVVGFRQFFYGIAMLDRYHGHGWQKWVQILGLSDETFAVNLEVEVPEHLDQQLVYSYISIYDYLAWVFGAGLGGVLGQQLAIQINGLDFIMTALFIVLALEQILKESNHISSISGFIITAICLLAIGQTYFLLLALLLMVIEYTWIYRRLQRRAGGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKU9</accession>
    <name>H4GKU9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Homoserine dehydrogenase</fullName>
            <ecNumber evidence="5 11">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PS3_19153</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-homoserine + NAD(+) = H(+) + L-aspartate 4-semialdehyde + NADH</text>
            <dbReference type="Rhea" id="RHEA:15757"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>L-homoserine + NADP(+) = H(+) + L-aspartate 4-semialdehyde + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 11">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="5 11"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122681.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19153"/>
    <dbReference type="EnsemblBacteria" id="EHS85019">
        <property type="protein sequence ID" value="EHS85019"/>
        <property type="gene ID" value="PS3_19153"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1678"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016204">
        <property type="entry name" value="HDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000098">
        <property type="entry name" value="Homoser_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="7 10 11" id="KW-0521">NADP</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="NAD_binding_3" evidence="14">
        <location>
            <begin position="10"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Homoserine_dh" evidence="13">
        <location>
            <begin position="139"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000098-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000098-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EHS85019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="429" mass="46121" checksum="B4908FC7D0707AB5" modified="2012-04-18" version="1">METIKIGLIGLGTVGAGVLEIISDPENQAKIENTIGRKLAVKTIVVHNPNKPRPVDTTGINVTTDVDEIVKDPEISIVVEVMGGIHPANEIIEELLQAGKNVVTANKDLIASNGPKLSALANANGCDLMYEASVAGGIPILRTITNSFTADQILEVKGIVNGTTNYILTQMREKQWSYDHALKRAQELGFAEADPTNDVAGIDAAYKMIILSLFAFGTQIKLSEFNVSGIENITDQDVKQAGQWGYVIKLMGVAKLVNEKLFVEVAPTLVPKSHPMAVVDNENNAVMVTGAAVGDTLFYGPGAGGLPTANSVLSDITSEAKNITLKTTGKSFNSYQHERQLADPADVKYSYYLAMTMPDVSGQMLAFTTLMTKVGASFERIEQTNREDKLADVAVITHAMTNQQLAELKQLVAESETLKLDAAYKVLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKY8</accession>
    <name>H4GKY8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable succinyl-diaminopimelate desuccinylase</fullName>
            <ecNumber evidence="5">3.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_21318</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + N-succinyl-(2S,6S)-2,6-diaminoheptanedioate = (2S,6S)-2,6-diaminoheptanedioate + succinate</text>
            <dbReference type="Rhea" id="RHEA:22608"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:58087"/>
            <dbReference type="EC" id="3.5.1.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 3/3.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.18" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122720.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21318"/>
    <dbReference type="EnsemblBacteria" id="EHS84754">
        <property type="protein sequence ID" value="EHS84754"/>
        <property type="gene ID" value="PS3_21318"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1731"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00021"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009014">
        <property type="term" value="F:succinyl-diaminopimelate desuccinylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010182">
        <property type="entry name" value="ArgE/DapE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01910">
        <property type="entry name" value="DapE-ArgE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="8">
        <location>
            <begin position="172"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS84754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="385" mass="41575" checksum="583AD012FA5AC297" modified="2012-04-18" version="1">MEKAERIQILRDLIQIQSVNGNELAVAQYLQKLFAQYKVEAQVLPFGDQRANLLLEVGDGANDQVLGITGHMDTVTTGDLEKWEYDPFAATVVGDRLYGRGAADMKSGLAAQVIAIIELVTAGQKLPGKVRLIATAGEEYGTPGANRLEAAGIAQDLSALIVGEPTSGNVIYAHSGSLNYRVTSVGKSVHSSRPADGVNAIDSLIDFCVGERQLFDDTPVDPYLGTVKHSVTIFKAGDQVNTIPDQAEINGNIRPTKAFPNDQVIAKLQALVDQINQAGQAQLSLEIIHSFWPVASDPDGKLVQLVLKASQEAYSSLAEHEQPKLTIINGATDASVFVKHHQDLPVVLLGADNWNISHQVNEYTTISSFLATIEAYKTIIKDYFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKZ0</accession>
    <name>H4GKZ0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC transporter ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_14236</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122722.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14236"/>
    <dbReference type="EnsemblBacteria" id="EHS84756">
        <property type="protein sequence ID" value="EHS84756"/>
        <property type="gene ID" value="PS3_14236"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1733"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033232">
        <property type="term" value="F:ABC-type D-methionine transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03258">
        <property type="entry name" value="ABC_MetN_methionine_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026253">
        <property type="entry name" value="ABC_MetN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041701">
        <property type="entry name" value="MetN_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018449">
        <property type="entry name" value="NIL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43166:SF5">
        <property type="entry name" value="PTHR43166:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09383">
        <property type="entry name" value="NIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00930">
        <property type="entry name" value="NIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4 6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="7"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="354" mass="39361" checksum="55EE793DF9CBAC56" modified="2012-04-18" version="1">MSDIIALDHVDVTFHPEKNQEVKAVQDVSLHVAKGDVYGVVGYSGAGKSTLVRTINLLQKPTQGRIEVNGTVLFDDQKQQISNKELQEKRRKIGMIFQHFNLLDETTVVENVMFALKHSKLKEKEVQAKAEKLLDLVGLSDKAKAFPAQLSGGEKQRVAIARALSNDPDILISDEATSALDPQTTNQILDLLKDLNEKLSLTIVLITHEMDAVKRVANKIAVMEYGKIIEQGTLHDIYLKPQQELTRQFVGGSLAAIDTLRAYNLEQLSASQRLYQLVFSAKNVGESVIISLYKECGVDVSMLYGNVEVLGNEPVGTLFVIVEGTDEQHAKALAYLKEKEIEVTRIDDRGIWHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKZ6</accession>
    <name>H4GKZ6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="7">4.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">OMP decarboxylase</fullName>
            <shortName evidence="7">OMPDCase</shortName>
            <shortName evidence="7">OMPdecase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pyrF</name>
        <name evidence="10" type="ORF">PS3_11207</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7 8">
            <text>H(+) + orotidine 5'-phosphate = CO2 + UMP</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 7 8">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the OMP decarboxylase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122728.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11207"/>
    <dbReference type="EnsemblBacteria" id="EHS84762">
        <property type="protein sequence ID" value="EHS84762"/>
        <property type="gene ID" value="PS3_11207"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1739"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01200_B">
        <property type="entry name" value="OMPdecase_type1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119:SF2">
        <property type="entry name" value="PTHR32119:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6 7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 7 8" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OMPdecase" evidence="9">
        <location>
            <begin position="5"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS84762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="242" mass="26044" checksum="CFEA1FBDF5406710" modified="2012-04-18" version="1">MEHFVPMVAIDVDTKEAAEQLFDQLSALADKPIVKIGMELYYANGPVIIKAAQAKGLKIFLDLKGYDIPNTIRRSMEILGQLGVDFTTVHAAGGSKMIQAAKEGLTAGAKTVNLPTPKLLAVTQLTSMDEDTMHREQGIPGALVDSVKRYAQLAEKAGADGVICSALEVKAIREVTQDDFLCVTPGIRPNLNQTDDQSRVVTPAQARQIGSNGIVVGRPITQSDDPVASYQTIYQAFMGETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKZ9</accession>
    <name>H4GKZ9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase C51 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_5932</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5932"/>
    <dbReference type="EnsemblBacteria" id="EHS84765">
        <property type="protein sequence ID" value="EHS84765"/>
        <property type="gene ID" value="PS3_5932"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1742"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007921">
        <property type="entry name" value="CHAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05257">
        <property type="entry name" value="CHAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50911">
        <property type="entry name" value="CHAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C51" evidence="1">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="136" mass="14600" checksum="10EC7A41BF692B58" modified="2012-04-18" version="1">MSEALAALEAQQGNTLYNGQCYGLSAWYAEQLGGPTMMGSGHEYAQDIGSDYDWASYGFTVTNYPSISDIKPGDIICWEAGGALSPGIYGHTGVVVSIDSDGNMVTLEQNAENGQVVSQYNRTYSQTTIKSVIHKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL06</accession>
    <name>H4GL06_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_8070</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122738.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8070"/>
    <dbReference type="EnsemblBacteria" id="EHS84772">
        <property type="protein sequence ID" value="EHS84772"/>
        <property type="gene ID" value="PS3_8070"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1754"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="9">
        <location>
            <begin position="295"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS84772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="1216" mass="137920" checksum="CC878E0C5B240B45" modified="2012-04-18" version="1">MGTLQFVIGKASRQHEAALVEDLIQRLQDPKQSKDRFFFLVPNHIKFETEVRILATLQSRLGYREQYAQSRVQILSFSRLAWYLLKNDPAYQSPRLSKMGPVMLVAQIIQQLKQTNPQQLGIFQGESDRPGFVQAVTEQIIAFQTANLTPDDLIDQPDSVVAQALQSQHQSPSQEIWLEKLRIISQIYERYLTLSADRLSNVDIEDLLVQKLHDPQASHDHFYLDRYTGQFSAKEQAIVEAMIQNGASTMMSLVADRAYPPHELPDEHNLYYQSAAQYQRLITFVDQQTKVDLLPPIMAAQLPLRVGNDLLAVETWMEAQAQFQTPEVKVDFQGQVSFLVTPTRLAEVDQIAAKIRQLVATKGYRYRDFLILTRHLASYENIIEPIFKRHQIPLFIDHQRSMANSPLVTMIEALFKIKQNYYQTADVMQLLKTELLTPQDVEHATFMEAVYQTENWCLKYGQGGKAWLRKAWQPIANESADLMITINQVRQYISREIAPFLDQLDQITTGRELATALYQFLVQQGVDQALLKWSQAALEAGDLTLMEQPQQVWQTFCNLLDEYVDLVGELTNEPKELLEEFIAMIEAAFGAAEYSQIPSTLDQVMISETGMAQTNQRSVVIMMGSTDDVMPEIKTTTGLLSDPDLDLLRQGLKADQFLPLSDVARSNHEPFINYLGMLSGTQRLIMTAAQLSGDESELTLSPYLIGLAQHLGQWDSNHQMVLGTQPAQIKPQAEFEQILPVISTPAVSMAYLLATRRLQADDQTWLSDGWQSLAVELNQLMPGRYQHLMASLDYHNRTTNLPAPLAVKLYGQPLGDDSHGQLMGSVSQLQTFYQNPYEYFLKYGLRLQPRDELEISIDKSGTFFHDVMDQFMRLVKQQSVDLADLVDDEALLKTLTDQAIQVAKTKQVSVMELAKNDQVAAYQVYSMEKVARTMTRILSLQAQHSDARAVAMEQPFGPRVTRPGDDNQVVLTPLQQQLPNGKVVTLRGRIDRVDQVVTDQGTDYIIVDYKSSKRKFDLIDAYVGTDLQMLAYLDVVQQNLSNGRAAGAIYLHLSDPQYKETDLKKNLTTVSLENHIYKGILLNQEAFLTSLDDGLTSKSPKGLVIDVNKLGKPKPGLNRSEQNNFQAKSGTSLVSPDQLEWLIKRNRWLIHQAASEILAGNVALFPVRRGAHSGLEYSDYLDIYQFDQMLDRYREITIDEDGLIKKMQAELEGDHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL29</accession>
    <name>H4GL29_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_19617</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122761.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19617"/>
    <dbReference type="EnsemblBacteria" id="EHS84659">
        <property type="protein sequence ID" value="EHS84659"/>
        <property type="gene ID" value="PS3_19617"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1781"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS84659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="233" mass="25314" checksum="FCE9DB9FA506DE7E" modified="2012-04-18" version="1">MAYIEVKHNTKTYPSGETTVDANHDISFEIEKGQLVIILGSSGAGKSTLLNILGGMDTNTSGQVMIDGHDIAQYSRKELTAYRRNDVGFVFQFYNLVPNLTAKENVELASQIVKDSMDAEQALASVGLADRTGNFPAQLSGGEQQRVAIARAIAKKPKLLLCDEPTGALDYETGKQVVQILQDMSRKDGSTVIIVTHNAALAPIGDQVIHIHDGQVKQIDRQDHPADIASLEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL45</accession>
    <name>H4GL45_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">30S ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpsO</name>
        <name evidence="7" type="ORF">PS3_4960</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="3 6">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 30S ribosomal subunit. Forms a bridge to the 50S subunit in the 70S ribosome, contacting the 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122777.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4960"/>
    <dbReference type="EnsemblBacteria" id="EHS84675">
        <property type="protein sequence ID" value="EHS84675"/>
        <property type="gene ID" value="PS3_4960"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1800"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_B">
        <property type="entry name" value="Ribosomal_S15_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_S15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005290">
        <property type="entry name" value="Ribosomal_S15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="S15_NS1_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23321">
        <property type="entry name" value="PTHR23321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="SSF47060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00952">
        <property type="entry name" value="S15_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS84675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="89" mass="10489" checksum="1E3DE218E532FD95" modified="2012-04-18" version="1">MAISKERKNEIIKEYATHEGDTGSTQVQVAVLTADINELNEHIRVHKHDYHSQRGLMKKIGHRRNLLAYLRRTDVQAYRELIKRLGLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL51</accession>
    <name>H4GL51_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endopeptidase La</fullName>
            <ecNumber evidence="1">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_18361</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="1"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122783.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18361"/>
    <dbReference type="MEROPS" id="S16.012"/>
    <dbReference type="EnsemblBacteria" id="EHS84681">
        <property type="protein sequence ID" value="EHS84681"/>
        <property type="gene ID" value="PS3_18361"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1806"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="3">
        <location>
            <begin position="233"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS84681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="351" mass="38929" checksum="BD427E8FEF835247" modified="2012-04-18" version="1">MKNQERRLIRRLILIIVGILVAMWFFLWPLNSYIETPGTADALKSYVTIKKHPDKWKGSYMLTSVYLQRARPVTYLWAKFMNPLATIESAEDVTGGQSDKTYDKVQNFYMQSAINQAISVAYKAAGQQYTKKYLGIYVLEVQKNSKFKKQIKVGDTITKVDGHHFNTAAGYQKYIAKQGIGHRLSVTYTHNGKTKTVTEPLVKLSTGKAGIGIILTDNVKVTTDIPVKVDPGEIGGPSGGLMFALQIYQQISGKDLRHGQQIAGTGTIAADGSVGEIGGIDKKVVAAHKAGATIFFAPYVKPTKELLKYEEQHMTNYQLAKKTAKKYAPNMKVVPVTSFQDAVDYLNEHAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL72</accession>
    <name>H4GL72_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ftsZ</name>
        <name evidence="10" type="ORF">PS3_9743</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the FtsZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122804.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9743"/>
    <dbReference type="EnsemblBacteria" id="EHS84702">
        <property type="protein sequence ID" value="EHS84702"/>
        <property type="gene ID" value="PS3_9743"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1835"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="PTHR30314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="SSF52490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="SSF55307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01134">
        <property type="entry name" value="FTSZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 6 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin" evidence="8">
        <location>
            <begin position="14"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin_C" evidence="9">
        <location>
            <begin position="208"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="316"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="316"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="22"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="109"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS84702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="420" mass="44171" checksum="D1336231F91152F2" modified="2012-04-18" version="1">MDKTANTFDNRGARIKVIGVGGGGGNAVNQMINEGVQGVEFIVANTDLQALEGSQAPVKIHLGPKLTRGLGAGSNPDVGMKAAQESEQEVAKALEGADMVFVTAGMGGGTGTGAAPVIAKLAKDSGALTVGVVTKPFSFEGKHRTDLANEGLEQMKKNVDTLVTIANDRLLEIIDKKTPMMEAFKKADDVVRQGVEGISDLIINPGYINLDFADIRHTMTNQGSALMGIGSSNGDNRAAEATKKAISSPLLEVSIDGAEHVLVNVTGGKDLSMFEAQEASSVIRQAANSNVDITFGMALDDTLNDEVRVTVIATGINGNGTSKNTSSQPTRFETPSQPAQPQRVSQPTSAPVQPSPQPEVSNDPFSGWNDPGNDVSDDLRSGNLRGAQKPSFSVQNDNLSNNNDNGEDLSRPAFFKNRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL87</accession>
    <name>H4GL87_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GTP pyrophosphokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_3028</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122819.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3028"/>
    <dbReference type="EnsemblBacteria" id="EHS84717">
        <property type="protein sequence ID" value="EHS84717"/>
        <property type="gene ID" value="PS3_3028"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1853"/>
    <dbReference type="UniPathway" id="UPA00908">
        <property type="reaction ID" value="UER00884"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015970">
        <property type="term" value="P:guanosine tetraphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05399">
        <property type="entry name" value="NT_Rel-Spo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007685">
        <property type="entry name" value="RelA_SpoT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04607">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00954">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RelA_SpoT" evidence="2">
        <location>
            <begin position="44"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS84717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="229" mass="26908" checksum="9BBC4D55397AA695" modified="2012-04-18" version="1">MIEDWKHFLLPYQQAVDELKVKLRGMRKQFQDQSAHSPIEFVTGRVKPVESIQEKMVRRHVQEDRLEQDMQDIAGLRIMCQFVEDIYQVVDLLRERTDITIIEERDYITNVKPSGYRSYHIVIEYPVQLVTGEKRILAEIQVRTLAMNFWATVEHSLNYKYQGEFPEAMAARLKTAAEAAFKLDEEMSEIREDIQEAQQLFSHGRGAEWTKSAIESQEDQNDQVERGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL88</accession>
    <name>H4GL88_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Competence protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_6379</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122820.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6379"/>
    <dbReference type="EnsemblBacteria" id="EHS84718">
        <property type="protein sequence ID" value="EHS84718"/>
        <property type="gene ID" value="PS3_6379"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1855"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010330">
        <property type="entry name" value="CoiA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06054">
        <property type="entry name" value="CoiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS84718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="335" mass="40552" checksum="FC9084EBFA171FE1" modified="2012-04-18" version="1">MLIAFNSQGQLIHASQAKAKVKYYCPETFVPMEMRLSSKNQPYFAKINHQGAAESQEHLLGKQQIYTWCQQQGWPAELEVPVNSIQQRIDVLANVKLQKIAFEFQCSPISIQTLKKRTTYYWRAGYNVYWFLGSPYRPISRKMKAKFTQWDQQQPVLYWWHVKQGKLSKQVMNLNNFKNNDLISRELNRLKWQRRHKTHSTLLNLAYLNHHQLWLCPAFLHNWGENWPLTSEHLLEWQLKILLQLERLPLKSQWTLEQWFQWVEQQTNWLPMPCLRVKQQQILHQRLINQLTQRWSQAGILKVTKQDIIFWQFPKWYGSEQEKKRHLSSWRWDQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL95</accession>
    <name>H4GL95_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Translocase subunit yajC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_12208</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YajC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12208"/>
    <dbReference type="EnsemblBacteria" id="EHS84594">
        <property type="protein sequence ID" value="EHS84594"/>
        <property type="gene ID" value="PS3_12208"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1871"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003849">
        <property type="entry name" value="Preprotein_translocase_YajC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33909">
        <property type="entry name" value="PTHR33909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02699">
        <property type="entry name" value="YajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01853">
        <property type="entry name" value="YAJCTRNLCASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01323">
        <property type="entry name" value="YajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00739">
        <property type="entry name" value="yajC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="87"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="87"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS84594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="128" mass="14091" checksum="1EC5437127D98291" modified="2012-04-18" version="1">MSSMSGIILIIFMIAFMYFFMMRPQQKQRKEHQEMVNSLQPGDEVVTIGRLHGVVSEVNASNQTVTIDCEGIYLTFDLNAIARVNRPTAAPTSNESSQSESTSESTSEAASETSEEVTSESESETKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLB5</accession>
    <name>H4GLB5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable tRNA sulfurtransferase</fullName>
            <ecNumber evidence="6">2.8.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Sulfur carrier protein ThiS sulfurtransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Thiamine biosynthesis protein ThiI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA 4-thiouridine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">thiI</name>
        <name evidence="9" type="ORF">PS3_11881</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + ATP + H(+) + 2 reduced [2Fe-2S]-[ferredoxin] = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24176"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:13337"/>
            <dbReference type="Rhea" id="RHEA-COMP:13338"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:136798"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-cysteinyl-[cysteine desulfurase]</text>
            <dbReference type="Rhea" id="RHEA:43340"/>
            <dbReference type="Rhea" id="RHEA-COMP:12157"/>
            <dbReference type="Rhea" id="RHEA-COMP:12158"/>
            <dbReference type="Rhea" id="RHEA-COMP:12908"/>
            <dbReference type="Rhea" id="RHEA-COMP:12910"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:90619"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ThiI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.4" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122847.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11881"/>
    <dbReference type="EnsemblBacteria" id="EHS84614">
        <property type="protein sequence ID" value="EHS84614"/>
        <property type="gene ID" value="PS3_11881"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1893"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140741">
        <property type="term" value="F:tRNA U4 sulfurtransferase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01712">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00021">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020536">
        <property type="entry name" value="ThiI_AANH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003720">
        <property type="entry name" value="tRNA_STrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02568">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00342">
        <property type="entry name" value="TIGR00342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="2 6 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="8">
        <location>
            <begin position="60"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="186"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="211"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS84614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="408" mass="45364" checksum="A0D545ECC747B91F" modified="2012-04-18" version="1">MKYDEIMVRYGELSTKGRNKKTFTDRLGENVRRALHGIGQVKVHARPDRLHIILNGADSDLVMERLAKVFGIQSFSPSLKVTKDFAAVAQAAIAMMGEQLSDGEKKTFKVETKRQDHQFELDTFQMNRQLGGALLKAFDGQLTVDVHHPDITIWVDVRLNGIFLTSEIIPGAGGLPVGTAGKGMMMLSGGIDSPVAAYLAMKRGVSLEMVHFYSPPYTSEQALAKSKQLTAKLAQYSGQIKFIQVPFTEIQETVKEQSPEGFLMTIQRRFMLRLATAIAKQRHGLAVFNGESLGQVASQTMNSMVAINDVTSFPVLRPVLSYDKTEIIKIAEQIDTYDLSILPYEDCCTVFTPPSPKTKPNLEKTRYYEQQLDVDGLMERALAGIKITEIHPGDDFLNANQDVFAELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHR9</accession>
    <name>H4GHR9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UV-damage repair protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_10543</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-Y family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000004">
        <property type="protein sequence ID" value="EHS87549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10543"/>
    <dbReference type="EnsemblBacteria" id="EHS87549">
        <property type="protein sequence ID" value="EHS87549"/>
        <property type="gene ID" value="PS3_10543"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.35"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11799">
        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UmuC" evidence="2">
        <location>
            <begin position="11"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="510" mass="57440" checksum="D4D1A75F0B98B947" modified="2012-04-18" version="1">MEVFIVSEQTFLAIDLKSFYASAECAALGLDPLNANLVVADESRTEKTICLAVSPALKAFGVPGRPRLFEVNQAVRKLNQQRTQALGHSLAKQKSIYREQLLAHPSLRLSFQIVPPRMQYYLNLSAEIYEIYLRYLEPKHIHVYSIDEVFIDITDYLHHHDTSAAELAKEIILTIQAETSITATAGIGTNMYLAKVALDIMAKKIPTDADGVRIAHLDELSYRQLLWAHQPLTDFWRVGRGYARRLKKLGLNTMGDIARASIGTLSDPLNAEVLYHEFGKNAELLIDHAWGFESATIRDIKQYRASDHGIYSGQVLPKPYPIPAARLVVSEMADDLALQLIKQQSLTDQITLHLSYDAQSLNQPFFDYNGPLSQDFYGRQVPKSAHGSRKLSDLTTSSRELKVAFDELFDEIANPKLLIRKITVIANHLVTETELAKIQRTQQLDLFSDPDQVAHQARQEQSHRDQDKNVQETILKIQNEFGKNALIKAGDLLEDATARKRHNEIGGHQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHU3</accession>
    <name>H4GHU3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_5700</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121627.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5700"/>
    <dbReference type="EnsemblBacteria" id="EHS87499">
        <property type="protein sequence ID" value="EHS87499"/>
        <property type="gene ID" value="PS3_5700"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.63"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032284">
        <property type="entry name" value="RecQ_Zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16124">
        <property type="entry name" value="RecQ_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="29"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="225"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="481" mass="54612" checksum="A028B892B076CAE8" modified="2012-04-18" version="1">MATIQAQLKQILHDKFGFDHFRPGQLETLEALLTGQPTLAVLPTGAGKTLLYQLPAYLMKGPILIVSPLISLMQDQLNRLRQQGLKRAAMLNSTISYQDRQLILNHLSEYQFIFTSPEMLAKETNLAAFKRQGVAMMVIDEAHCVSQWGPDFRPEYLLLKEVIKSLQPAKLLMLTATATPNTRQDIIDRLGLTSQQVHQIIQSVNRENIFLAVEKVPNPDEKRQRLVDLLQQLGGQGIIYFSSRALANEMTAYLSAVTDYQVAAYHAGISDVDRYRIQQQFMHDELQIVCATSAFGMGIDKDNIRYVIHYHLPNSLENYVQEIGRAGRNGQHALAILLYAPGDIQIPRLLTSVEVPPISVMEQFNNGGVPLEALGPQAEVIAFYLSHQTSPQVIKQLFQEQEQKVQARLDKMMDYLQTQTCRRQIILNYFGESKINQSQICCDIDLPHWTVSKLDLPAEVNRSLPNSQLDWRRRLNSFFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHV2</accession>
    <name>H4GHV2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Triphosphoribosyl-dephospho-CoA synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_3170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA + apo-[citrate lyase ACP] = diphosphate + holo-[citrate lyase ACP]</text>
            <dbReference type="Rhea" id="RHEA:16333"/>
            <dbReference type="Rhea" id="RHEA-COMP:10157"/>
            <dbReference type="Rhea" id="RHEA-COMP:10158"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61378"/>
            <dbReference type="ChEBI" id="CHEBI:82683"/>
            <dbReference type="EC" id="2.7.7.61"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>3'-dephospho-CoA + ATP = 2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA + adenine</text>
            <dbReference type="Rhea" id="RHEA:15117"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61378"/>
            <dbReference type="EC" id="2.4.2.52"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121636.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3170"/>
    <dbReference type="EnsemblBacteria" id="EHS87508">
        <property type="protein sequence ID" value="EHS87508"/>
        <property type="gene ID" value="PS3_3170"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.77"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050519">
        <property type="term" value="F:holo-citrate lyase synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046917">
        <property type="term" value="F:triphosphoribosyl-dephospho-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051191">
        <property type="term" value="P:prosthetic group biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002736">
        <property type="entry name" value="CitG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005551">
        <property type="entry name" value="CitX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30201">
        <property type="entry name" value="PTHR30201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01874">
        <property type="entry name" value="CitG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03802">
        <property type="entry name" value="CitX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="473" mass="52770" checksum="2A8D840696477F2C" modified="2012-04-18" version="1">MKIPEIFATGQEVDTQTIREHAQARFQELSMLVSSNPDWTVVSVALNIPGSIKTNQLIQNFFQNGVKRFENSLIARGLTFKCQLQDDLSPTGPERGYLIKGNPLLIKSHTSHFEEANPSANLFNLDVLAVLNGEIRTLPREAVKAPARRCLICDKPAKFCQAQQRHSIEELQTQVDRLIQLQLEADRQGQMAQQFGKLATQALLEEVAIWPKPGLVDPLEHLSHPDMDHFTFIKSALSLQDYFTKCALVGLTTHEDNYQLVFDEIRQFGLVAEREMNLATAGVNTHKGAIFTLGIIATAAGIIINRDQWSFDQLKLTIQQMLAKLMHDDLQSQKEPTEATAGQQQYHQYGLGGVREEAALGYPTVFNYGLPAYQAAADQSSNDRGVTTLLAIARHTMDSNLIKRAGNMDILQERDHWIDEIDAAGGITTDLGRQKLNEMSVEFARRRLSMGGSADLLSATLFIAMIDNLQNKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHV4</accession>
    <name>H4GHV4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP cyclohydrolase 1 type 2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_10154</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121638.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10154"/>
    <dbReference type="EnsemblBacteria" id="EHS87510">
        <property type="protein sequence ID" value="EHS87510"/>
        <property type="gene ID" value="PS3_10154"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.79"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="PTHR13799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="SSF102705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00486">
        <property type="entry name" value="YbgI_SA1388"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="275" mass="30517" checksum="2805C7A568B81FB3" modified="2012-04-18" version="1">MTTGTELINRFEQFASPQLAESWDHVGLQIGNPDRPIQKLMTTLDVRPEIVREAIDQQVDFIFAHHPIMFKPATDLDVRNPQNQMYADLLSHQITVYAAHTNLDTANGGMNDWLADQLELTNCEPLVAAGQDPVTKQPVGMGRVGQLPNAMTIDEFTRHCQSVFGVSGLRLVKPASFNSAIERVAVLGGAGGSFYQQALQAGADAYVTGDISYHTGHDIIANGLVALDPGHHIEAVATHRLAKLFKQWQLNHHWKFKIIENQINTDPFEFICKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHZ3</accession>
    <name>H4GHZ3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BglG family transcriptional antiterminator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_5710</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000006">
        <property type="protein sequence ID" value="EHS87472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121677.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000006.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5710"/>
    <dbReference type="EnsemblBacteria" id="EHS87472">
        <property type="protein sequence ID" value="EHS87472"/>
        <property type="gene ID" value="PS3_5710"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.123"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007737">
        <property type="entry name" value="Mga_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011608">
        <property type="entry name" value="PRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036634">
        <property type="entry name" value="PRD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036095">
        <property type="entry name" value="PTS_EIIB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013011">
        <property type="entry name" value="PTS_EIIB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05043">
        <property type="entry name" value="Mga"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00874">
        <property type="entry name" value="PRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52794">
        <property type="entry name" value="SSF52794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63520">
        <property type="entry name" value="SSF63520"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51372">
        <property type="entry name" value="PRD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51099">
        <property type="entry name" value="PTS_EIIB_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PRD" evidence="5">
        <location>
            <begin position="292"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIB type-2" evidence="4">
        <location>
            <begin position="404"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="494" mass="56806" checksum="20DD2FBF54E7A8E3" modified="2012-04-18" version="1">MIKKREEVIVALLKADDFLTAHQLAQDLKLSTKTIYRAVKEINQSFGQTVIISERGKGLLLDYDRYLMLSNELPLAADFIGVTPVERRNDILTKLLFTSPLTCRVDELYEPYHVSYDLMQYDLQHLTKILTTYHLKLLRRGNRLSIAGDEQAIRRLINKVLMQSNAMNVETIRDFASRFPDMGNYDQQFLTAQLELIQRSFQTTIPYPYNINIFSHLYVLMKRYRTGKVQSSQSVQLSPQDQALIHAQPIILKVAKNVIQNVGEYVHHSIEPAEINSLVEYLLSMRYSHEVIYDPHSTPLCNQLVEIFLNGFDLDEHATGIQTLRNDLVSHLRPMMNRLNNQIQVANKLLPDIKLEYGELFARVQHLAQQAQTSLSLPWSIDEDEVGFITLYFAKYFEETEFHQRALVMCASGVGTSKLLCAKVHKRFPNLEIVGITSKAQYEAHPERYRGIDLIITTVEVQAHNQERVMLTNALFTVQDQKRLGQLLGVKDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI04</accession>
    <name>H4GI04_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribosome-associated heat shock protein implicated in the recycling of the 50S subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_19959</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000007">
        <property type="protein sequence ID" value="EHS87455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121688.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000007.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19959"/>
    <dbReference type="EnsemblBacteria" id="EHS87455">
        <property type="protein sequence ID" value="EHS87455"/>
        <property type="gene ID" value="PS3_19959"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.136"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025490">
        <property type="entry name" value="RNAbp_HP1423"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038881">
        <property type="entry name" value="RNAbp_HP1423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="S4" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="90" mass="10446" checksum="E84DD4BACCE2E599" modified="2012-04-18" version="1">MRLDKYLKISRIIKRRPIAKKIADQGRIMVNDKVAKSSSSVVTGDVVTIKFGNKTLVVKVKQLLDSTKKEDAEQMYEILEEKYEQDFREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI29</accession>
    <name>H4GI29_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">D-alanine--D-alanyl carrier protein ligase</fullName>
            <shortName evidence="5">DCL</shortName>
            <ecNumber evidence="5">6.2.1.54</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">D-alanine--poly(phosphoribitol) ligase subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">D-alanine-activating enzyme</fullName>
            <shortName evidence="5">DAE</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">dltA</name>
        <name evidence="8" type="ORF">PS3_10167</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D-alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + D-alanine + holo-[D-alanyl-carrier protein] = AMP + D-alanyl-[D-alanyl-carrier protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:55132"/>
            <dbReference type="Rhea" id="RHEA-COMP:14102"/>
            <dbReference type="Rhea" id="RHEA-COMP:14103"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57416"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:138620"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cell wall biogenesis; lipoteichoic acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ATP-dependent AMP-binding enzyme family. DltA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.54" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000011">
        <property type="protein sequence ID" value="EHS87439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10167"/>
    <dbReference type="EnsemblBacteria" id="EHS87439">
        <property type="protein sequence ID" value="EHS87439"/>
        <property type="gene ID" value="PS3_10167"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.169"/>
    <dbReference type="UniPathway" id="UPA00556"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047473">
        <property type="term" value="F:D-alanine [D-alanyl carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070395">
        <property type="term" value="P:lipoteichoic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05945">
        <property type="entry name" value="DltA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00593">
        <property type="entry name" value="DltA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010072">
        <property type="entry name" value="DltA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044507">
        <property type="entry name" value="DltA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01734">
        <property type="entry name" value="D-ala-DACP-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="6">
        <location>
            <begin position="10"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="7">
        <location>
            <begin position="421"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="155"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>D-alanine</name>
            <dbReference type="ChEBI" id="CHEBI:57416"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="296"/>
            <end position="301"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>D-alanine</name>
            <dbReference type="ChEBI" id="CHEBI:57416"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="509" mass="56640" checksum="BB2D4B1B4C925A0F" modified="2012-04-18" version="1">MMNNLIWSIDQVASQHPTTIAYDEMGRLNTYQDLKNYSDALATWLDQNSALDLHQPILFYGDHQFTMVAGFIGSLKAGHSYIPVETGTASTRLASIIETGQPGLVVAIDDFPAGEVNYNGPVLTSDQLTEIMHHQVNYQLDHVVDGDQLFYILFTSGTTGSPKGVEISHDNINSFARWMLSDDFKIPQGQTFLGQPPYSFDLSGMFWLPALLSGGTIRALPSEVVKNFGQLFTVLPSLDLNVYVSTPSLADMLMLSPDFSEAKMPGLTHFLFCGEELTVNTAKRLKERFPHAHIFNTYGPTEAAVAITSVEITQEMVDTLKRLPVGKVKANTTTSIWDGDQEITTPKTEGELVITGDSVARGYLNNPTKTQQNFFKLNGIQSYRTGDAAMIDEDGMHYIVGRMDFQIKLHGFRIELDEVRSSIETSPLVKQAVAVPKYDANHRATHLIAYIIPNDPVDDEAEFTKALRNDLKDRVMEYMMPTQFVYVDDFPKSANGKIAIKQMIAEANQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI37</accession>
    <name>H4GI37_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">ATP-dependent Clp protease proteolytic subunit</fullName>
            <ecNumber evidence="5 8">3.4.21.92</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Endopeptidase Clp</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">clpP</name>
        <name evidence="11" type="ORF">PS3_14622</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 9">Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Fourteen ClpP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 10">Belongs to the peptidase S14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.92" evidence="5 8"/>
    <dbReference type="EMBL" id="AICN01000012">
        <property type="protein sequence ID" value="EHS87429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121721.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000012.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14622"/>
    <dbReference type="MEROPS" id="S14.001"/>
    <dbReference type="EnsemblBacteria" id="EHS87429">
        <property type="protein sequence ID" value="EHS87429"/>
        <property type="gene ID" value="PS3_14622"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.179"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00444">
        <property type="entry name" value="ClpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033135">
        <property type="entry name" value="ClpP_His_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018215">
        <property type="entry name" value="ClpP_Ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="PTHR10381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00493">
        <property type="entry name" value="clpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00382">
        <property type="entry name" value="CLP_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00381">
        <property type="entry name" value="CLP_PROTEASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 8" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 8" id="KW-0720">Serine protease</keyword>
    <feature type="active site" evidence="6">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 7">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="196" mass="21518" checksum="7864A6362D92F501" modified="2012-04-18" version="1">MNLVPTVIEQSSRGERAYDIYSRLLKDRIIMLSGPIEDDMANSIVAQLLFLDAQDSTKDIYLYINSPGGVVTSGMAIYDTMNFIKSDVQTIVIGMAASMASVLVSSGAKGKRFGLPHSQVMIHQPSGGAQGQQTEIQIAAEEILKTRKMINEILAKNSGQPFDKVEQDTERDHYLTAQEAVEYGLLDGIMDSNELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI54</accession>
    <name>H4GI54_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Inner membrane insertion protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_13039</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121738.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13039"/>
    <dbReference type="EnsemblBacteria" id="EHS87393">
        <property type="protein sequence ID" value="EHS87393"/>
        <property type="gene ID" value="PS3_13039"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.202"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="Membr_insert_YidC/Oxa1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="PTHR12428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03592">
        <property type="entry name" value="yidC_oxa1_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="263"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="270"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="286"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="312" mass="34989" checksum="DC9108B50D7E4226" modified="2012-04-18" version="1">MKHKRLFTLSGLTLLGLVLSGCVRTTKSGKPYGIIYDYLAKPMQHFMEWLAGIFGGNYGLAIIALTIIIRLILFPIMFNQMKKSTMMQERMSKLQPHLKELQERQKNAKTPEEQALVGQQMMSFYRDNNVSLTGGIGCLPLLIQLPIFAALYAAIRYSPELSSSPFFGIPLGKPSIIMALLAFVTYAIQGVLSLQGVPQEQRAQMRSAMLISPIMILFISWTSSAGLGLYFFAGGIFAILQTVLINLYRPRIRKQIEEELKDNPIKVPDPIQPANSQSDVQATIDQLRNDHQKADHEHHPKGRNAGKQHHQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI61</accession>
    <name>H4GI61_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA(Met) cytidine acetate ligase</fullName>
            <ecNumber evidence="1">6.3.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tmcAL</name>
        <name evidence="2" type="ORF">PS3_9228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of elongator tRNA(Met), using acetate and ATP as substrates. First activates an acetate ion to form acetyladenylate (Ac-AMP) and then transfers the acetyl group to tRNA to form ac(4)C34.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + cytidine(34) in elongator tRNA(Met) = AMP + diphosphate + N(4)-acetylcytidine(34) in elongator tRNA(Met)</text>
            <dbReference type="Rhea" id="RHEA:58144"/>
            <dbReference type="Rhea" id="RHEA-COMP:10693"/>
            <dbReference type="Rhea" id="RHEA-COMP:10694"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TmcAL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.-" evidence="1"/>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121745.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9228"/>
    <dbReference type="EnsemblBacteria" id="EHS87400">
        <property type="protein sequence ID" value="EHS87400"/>
        <property type="gene ID" value="PS3_9228"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.214"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01539">
        <property type="entry name" value="TmcAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008513">
        <property type="entry name" value="tRNA(Met)_cyd_acetate_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37825">
        <property type="entry name" value="PTHR37825"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05636">
        <property type="entry name" value="HIGH_NTase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1" id="KW-0820">tRNA-binding</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="385" mass="44092" checksum="A81540CCD916702C" modified="2012-04-18" version="1">MQALGLVAEYNPFHNGHCYHLQQARQLTGADVVVAVMSGNFTQRGEPTILDKWQRTQAALANGVDLVVELPVFNAIQPAHRFAEGALRLLNDLQVVTVVFGAEHPAWNFDSLVAAEARFKASDFKDYHATYATQFNQQLSAQTGYSLTDPNDILAFAYYKARQEGHYTMNLLPIQRRGNDYHDQTITGRLASASAIRAAWSDLKTIKNTVPATTVSDLAQLQRLPSWDLLYPILRNQLIQTPVEWLQQIYQMGEGIEYRMKEAAQRALTYADFIRLVKTKRYTYAYLSRLSLYVILNISKVEHDRHQQVPYHHILGFNQVGQAYLNQVKKQLDFPLIVKVNRDERDGRFNLDYRAGKLYQNFTKVEQDMKHAPIRTVKEITLTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI65</accession>
    <name>H4GI65_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable nicotinate-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="6">2.7.7.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Deamido-NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Deamido-NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Nicotinate mononucleotide adenylyltransferase</fullName>
            <shortName evidence="6">NaMN adenylyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nadD</name>
        <name evidence="8" type="ORF">PS3_4386</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 6">Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + H(+) + nicotinate beta-D-ribonucleotide = deamido-NAD(+) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22860"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="EC" id="2.7.7.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 6">Cofactor biosynthesis; NAD(+) biosynthesis; deamido-NAD(+) from nicotinate D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NadD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.18" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121749.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4386"/>
    <dbReference type="EnsemblBacteria" id="EHS87404">
        <property type="protein sequence ID" value="EHS87404"/>
        <property type="gene ID" value="PS3_4386"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.218"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00332"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004515">
        <property type="term" value="F:nicotinate-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02165">
        <property type="entry name" value="NMNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00244">
        <property type="entry name" value="NaMN_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00482">
        <property type="entry name" value="TIGR00482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4 6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="7">
        <location>
            <begin position="28"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="211" mass="24287" checksum="E361CD7591BD2B80" modified="2012-04-18" version="1">MPIKSAVRMIPKVEVQPLTSFRHRRIGLYGGTFNPVHNAHLLVADQVGKTLGLDRVDFLPDLIPPHVDQKSAVAAEYRVDMLKLAIDDNPYLGIEMAEINRGGISYSYDTIKELKERHPENDYYFIIGGDMVDYLPTWYRIDDLMKMVNFVGVRRQGAENNTNYPVIWVDVPTVDFSSSAIRERIRNRQSIKYMVPAGVENYIKEHQLYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI87</accession>
    <name>H4GI87_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Penicillin binding protein 2A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PS3_15377</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15377"/>
    <dbReference type="EnsemblBacteria" id="EHS87426">
        <property type="protein sequence ID" value="EHS87426"/>
        <property type="gene ID" value="PS3_15377"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.246"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Transgly" evidence="10">
        <location>
            <begin position="83"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="9">
        <location>
            <begin position="357"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="678"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="722" mass="79445" checksum="B851DE49490056C1" modified="2012-04-18" version="1">MTWFKEHPGPVLKNWWRKGSNVVFHQWRRFNLTRWLIIVFLTLFLGMSIYLTIVAKTANVKDLKARLETNTQIYDKNGSSAGSLYDQKGTYVPLSKISKNVQNAVLSTEDRNFYHEHGFSFKGLARAAFLLVRNRLTGSSAISGGGSTLTQQLVKNAFLTQQQTFSRKLKEIFISIEVENQYSKKEILTMYLNNAYFGNGVWGVQDAAERYFDTDAADLTVPQAATLAGMLTSPGIYDPVKYPSATKKRRNVVLQLMVENNKLSQSAANQYKKTSLTVTNGYQSSEDTYKYPYYFDAVISEAISDYGLTESEVMNNGYKIYTTLDQNQQAAMQSTYDDSSNFPSASSDGTIVQSASIAMNPKTGGVTAVVGGRGTHVFRGYNRSTQMRRQPGSTIKPIVVYAPALEKGYSYDSTVQDKLQSYGTNKYTPKNYDGTYSGSVPMYEALYKSLNAPAVWLLNKIGVNAGYKSAQDFGLPVESGDKNLALALGGMTKGVSPQQLARAYSAFASGGTLPTAHYITKIVDASGNEVPRKTKLTKKRIISTKIANQMTSMMIDVFKLGTGKNAKPANYTIAGKTGTTNSGLSNDNDRDKWIVGYTPDLVVATWEGYDSTTDEQQLSDLESSDIYSLYKTEMSSLLPYTKGTSFTVKAASTRANSASSNSGTQKLWEQATSWWNKLTGNSNSDSTDTTDTTDTSDSTDESSSSGIGDQISNAWNQLTSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIA7</accession>
    <name>H4GIA7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Organic hydroperoxide resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OsmC/Ohr family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121791.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13403"/>
    <dbReference type="EnsemblBacteria" id="EHS87328">
        <property type="protein sequence ID" value="EHS87328"/>
        <property type="gene ID" value="PS3_13403"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.272"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019953">
        <property type="entry name" value="OHR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33797">
        <property type="entry name" value="PTHR33797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="SSF82784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="140" mass="15796" checksum="B245DB1F73AA6A92" modified="2012-04-18" version="1">MDFKSIYQTTVENDMGILGETKGDLVLRTSSPLSEMPGTNPEQLVGLAYATCLNATIEAEEARRDLVHNSQVRVQVDLGKDVEGYQFKLLVLVRIPEVNRTVAEEILEIAKRRCPMEKLLGQHPNVTVKLVDQFKSRREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIA8</accession>
    <name>H4GIA8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glycerol-3-phosphate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_11832</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121792.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87329">
        <property type="protein sequence ID" value="EHS87329"/>
        <property type="gene ID" value="PS3_11832"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.273"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000849">
        <property type="entry name" value="Sugar_P_transporter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002808">
        <property type="entry name" value="Hexose_phosphate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="419"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="31"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="449" mass="49101" checksum="9C73F61B04942AAF" modified="2012-04-18" version="1">MIPFLRPAPDASKKVPAEKVVSSYRWHQTGVLLSLCIGYIGYYIIRLIFTTEQNDIMKDYGFTTAQVGLILSCFGIGYGISKLFMGALSDKSNTIRYLATGLIVSAILNVGLGATRNFYLMMILMLVMSIAQGMGAAACQRTIQLWWGKRWRGTVYSIWSSAHNAGAFCCVAVVQLAVFMFSGSIQAVFYTASVVSVIIALFVLWAGSERPAAVGLPSISEYTGDEVVLDGGETSSDDLTDLSLWQIFVQYILKNPMVWAITLTSMSLYLVRYGVMSWIPSYLVQSKGFSPDFAKWLVGIFELAAVPGVILMGAISDFLKDRRALVCLICVLGLVACLTTYFYSTSHVLIVTVLFIMGTLIYAPLTLVGLMVNEAVPKFAVGSSTGFMGFFQYIFGETLATALIGILVAQYGWVASNAVLYGASALSLLLLIYIMIHEARMKRLAKHAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIB3</accession>
    <name>H4GIB3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Ribokinase</fullName>
            <shortName evidence="10">RK</shortName>
            <ecNumber evidence="3 10">2.7.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rbsK</name>
        <name evidence="13" type="ORF">PS3_18751</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + D-ribose = ADP + D-ribose 5-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13697"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:47013"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="10">Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the carbohydrate kinase pfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.15" evidence="3 10"/>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121797.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18751"/>
    <dbReference type="EnsemblBacteria" id="EHS87334">
        <property type="protein sequence ID" value="EHS87334"/>
        <property type="gene ID" value="PS3_18751"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.282"/>
    <dbReference type="UniPathway" id="UPA00916">
        <property type="reaction ID" value="UER00889"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004747">
        <property type="term" value="F:ribokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019303">
        <property type="term" value="P:D-ribose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01174">
        <property type="entry name" value="ribokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01987">
        <property type="entry name" value="Ribokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011877">
        <property type="entry name" value="D_ribokin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02152">
        <property type="entry name" value="D_ribokin_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="12">
        <location>
            <begin position="3"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="40"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="221"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="252"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS87334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="306" mass="32495" checksum="396A84A49D026068" modified="2012-04-18" version="1">MANRVAVLGSLNVDTTYRVVRFPEPGETISAISRSSAPGGKGANQAVAAARSGAKTYFIGSVGNDDEGKLMRECLDDERIYTAHLTTNMDHGTGTALVTLNEEGQNNIMVYGGANQAMTTSVLDGTDQVFEDVDFMIAQFETPQAVTLEAFKRAKAHQVVTILNPAPASQLIPELAQYVDVIAPNETEAELLTGVKLTDEASLDLIAQHFQAMGIPVVLITLGKRGVYYATKDQAGIVPAFTVTAVDPTAAGDTFIGALASQLSTSLDNLDEAITYAQRASSLTVQKYGAMPSIPMGFEVEEALKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIC6</accession>
    <name>H4GIC6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribose-5-phosphate isomerase A</fullName>
            <ecNumber evidence="2">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Phosphoriboisomerase A</fullName>
            <shortName evidence="2">PRI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpiA</name>
        <name evidence="3" type="ORF">PS3_6136</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121810.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6136"/>
    <dbReference type="EnsemblBacteria" id="EHS87347">
        <property type="protein sequence ID" value="EHS87347"/>
        <property type="gene ID" value="PS3_6136"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.307"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00170">
        <property type="entry name" value="Rib_5P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020672">
        <property type="entry name" value="Ribose5P_isomerase_typA_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="28"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="84"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="97"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="226" mass="24557" checksum="5FB8395AF69202C3" modified="2012-04-18" version="1">MNQNDLKALVGQAAVDQIESGMVVGLGSGSTVAYLIKALGKRMQEEPLQIETVTTSRQTMQLAQAVGIDCRQIDEVRQIDLTIDGADEVDADFQGIKGGGGALLWEKIVNQSSTRNVWIIDESKLVDRIGRFGVPVEVIPFGSQKLFQILADRGYHPQWRMQGDQLFKTDEGNQIIDLKTGVIAHPHALARDLASTAGIVEHGLFLDAVDLVIVGRSSGVELMNAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIE9</accession>
    <name>H4GIE9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LacI family transcriptional regulator LacR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_5138</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121833.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5138"/>
    <dbReference type="EnsemblBacteria" id="EHS87370">
        <property type="protein sequence ID" value="EHS87370"/>
        <property type="gene ID" value="PS3_5138"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.345"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00036">
        <property type="entry name" value="HTHLACI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="3"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="320" mass="35229" checksum="DFAC9D061490839B" modified="2012-04-18" version="1">MAVTLKNIADEAGVSLATVSRVLNNDPQLSVSPETRERIVTIAQRLDYNKRRRQSPINRRLAIVEWYSTEHELSDLYYLNLRLNVERAAQEAGYSTATVFAGNLTSLADPVDGIVAIGKYSARQLEKMKRYADVIVDVDGDALAQSIPCVVPDFVGGITQAVDYLAPQHQRIGMIAGQETTGDQETVVDLRLKTFVNLMQERKRPDNLTWLGDYSDHSGYEQMQAILQLPVDQRPTAVIVANDAMSIGANRALQEASAAGIKLISFGDVAGVANYIYPPLDAIHVPTDQMAMIGIDLISKYMNNQLAPERVVVGTQLVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIF3</accession>
    <name>H4GIF3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_20899</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121837.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20899"/>
    <dbReference type="EnsemblBacteria" id="EHS87374">
        <property type="protein sequence ID" value="EHS87374"/>
        <property type="gene ID" value="PS3_20899"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.349"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00865">
        <property type="entry name" value="PEBP_bact_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005247">
        <property type="entry name" value="YbhB_YbcL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30289">
        <property type="entry name" value="PTHR30289"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="SSF49777"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00481">
        <property type="entry name" value="TIGR00481"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="168" mass="18460" checksum="76D8791F803B1D86" modified="2012-04-18" version="1">MKISVPLINGLLPDQYGKYAPEADLLADHPVHSFPIDITEAPAATKAYALVFIDYDSTPVCGFTWIHWLATNLPATWTQIPDDASRKLADQFVQGNNSNAGSLVNGDPQIITGYVGPQPPDQNHDYTLTVYALDQELPLSKGYWLNEFLKAAQGHILEKAKLTIPSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIG3</accession>
    <name>H4GIG3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Single-stranded DNA-binding protein</fullName>
            <shortName evidence="2">SSB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_5348</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121847.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000017.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5348"/>
    <dbReference type="EnsemblBacteria" id="EHS87284">
        <property type="protein sequence ID" value="EHS87284"/>
        <property type="gene ID" value="PS3_5348"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.361"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04496">
        <property type="entry name" value="SSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00984">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011344">
        <property type="entry name" value="ssDNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302">
        <property type="entry name" value="PTHR10302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00621">
        <property type="entry name" value="ssb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="136"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="136"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="225" mass="24529" checksum="7CF5E87C818FBAD0" modified="2012-04-18" version="1">MLNRAVLTGRLTRDPELRYTNSGTAVARFSIAVDRQFRNQQTGEREADFINCVIWRKSAENFVNFTHKGALVGIDGRITTSNYQNQQGQRIYRTEVTVENFALLEPRSARDNQAGNNQYNNGGYYNNGANNGFNANQGQAAFGGPANNFGATPQDNGNANDNPFGGQGLFGENTTPIDTDSLPFANSSNEGSASDAIDINDQTALKNDQANDKDDDQNSLSDVPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIH0</accession>
    <name>H4GIH0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Membrane protein insertase YidC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Foldase YidC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Membrane integrase YidC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Membrane protein YidC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">yidC</name>
        <name evidence="12" type="ORF">PS3_16418</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the OXA1/ALB3/YidC family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121854.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000017.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16418"/>
    <dbReference type="EnsemblBacteria" id="EHS87291">
        <property type="protein sequence ID" value="EHS87291"/>
        <property type="gene ID" value="PS3_16418"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.371"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01811">
        <property type="entry name" value="YidC_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="Membr_insert_YidC/Oxa1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023060">
        <property type="entry name" value="YidC/YidC1/YidC2_Firmicutes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="PTHR12428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00701">
        <property type="entry name" value="60KDINNERMP"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03592">
        <property type="entry name" value="yidC_oxa1_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008959327" description="Membrane protein insertase YidC" evidence="11">
        <location>
            <begin position="23"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="251"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="251"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="277" mass="32122" checksum="E5F7232586B60C8C" modified="2012-04-18" version="1">MKKQHKKLASIFSVLGLTVLLAACSNKPITSHSTGIWDHYIVYNCSQFIIWLSKIFGGYGMGIIIFTILIRIIILPLMYYQSKSMVKTQELAPKLKELQKKYSSRDRETMQKLQAEQQKLYREAGVNPFASMLPLLVQLPVMWALYQAIWRTNVLKTGSFLWLQLGKTDPYFVLPVLAAVFTFISSWLSMASMPEKNSMTTSMTYIMPIFIFIMALQFSAAITLYWVVSNAFQVVQVLLVQNPFKIRREREAKQKAERDHQRKIEKAKRKAYQSKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIH4</accession>
    <name>H4GIH4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Asparagine synthase (Glutamine-hydrolyzing)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_14228</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asparagine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121858.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000017.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14228"/>
    <dbReference type="EnsemblBacteria" id="EHS87295">
        <property type="protein sequence ID" value="EHS87295"/>
        <property type="gene ID" value="PS3_14228"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.375"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_Synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="8">
        <location>
            <begin position="2"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="5">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="356"/>
            <end position="357"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="7">
        <location>
            <position position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="636" mass="73316" checksum="635FF0A3C76954A2" modified="2012-04-18" version="1">MCGFVSFVNDEAPQLKDNEIQTMMKRIVHRGPDDANHYVDEHVALGFRRLSFIDVKSGRQPIFNEDESKAIIFNGEIYNFKSLREKLVEAGHIFRTHADTEVILHGYEEWGADIAKKLRGMFAFIIWDRQTNTMFGARDHFGIKPLYYAQMNGTFFVGSEIKAFLDHPSFKMELNKAALKPYLTFQYPATRETFFKRVFKLPEGHYFHYQDGKFEMAQYWDVDFDPEEESFGEAVDKIEAAVDESVKAHSFADEGIKVGSFLSAGVDSSYVTAMMKPDNTFSIGFDSGYDETKQARELAAKLGLKNTDKTLTNEEAFNAFPKIQYYLDEPDSNPSVVPLYFLTKLAKDNGYKAMLSGEGADELFAGYTDYGFNTKSKAIRWFADQLKKLPQKQRHSLSKALKKSPNFPGRLHLYHALAPANEDFIGQAYIFSPAEAADLLQEDYNCGPTIAEIMDPLYDKVASKDIDEVAKKQYMDVHQWMPGDILLKADKMSMANSLELRVPLLDRKVMDVARTTPSKYLFNYKGTKWAFRMAANRHLPEEWATRPKMGFPVPIREWLHQEKYYKEVRAMFAEDFVNEFFDQAKLLQLADDNFAGKIDGRRKLWTIYTFLVWYKLYFIDHYQPDESVIDQAQQVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIH8</accession>
    <name>H4GIH8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0210 protein PS3_8587</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_8587</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0210 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121862.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000017.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8587"/>
    <dbReference type="EnsemblBacteria" id="EHS87299">
        <property type="protein sequence ID" value="EHS87299"/>
        <property type="gene ID" value="PS3_8587"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.379"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08025">
        <property type="entry name" value="RNR_PFL_like_DUF711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01221">
        <property type="entry name" value="UPF0210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007841">
        <property type="entry name" value="UPF0210"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37560">
        <property type="entry name" value="PTHR37560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05167">
        <property type="entry name" value="DUF711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="447" mass="46758" checksum="49FA8FB1FE4842C4" modified="2012-04-18" version="1">MDNRRIFETVNMIANENLDLRTTTMGISLLDCIDGDSDRACQKIYDKITTKAQNLVKVAEDIETEFGIPIANKRITVTPISLIAAASGDEDYVKYALTLEKAAEAVGVDLIGGFSALVHKGYQNGDRTLIKSIPEALHQTSRVCASVNVGSTRSGINMDAVKEMGEVVIQNEALDPLLNGRLVIFCNAVEDNPFMAGGFHGVGESDVALNVGVSGPGVVKTALEKVRGKSMDVVAETIKQTAFKVTRMGQLVGQEASERLGVDFGIVDLSLAPTPAQGDSVANILEEIGLESVGTHGTTAALAMLNDAVKKGGIMACSHVGGLSGAFIPESEDAGMIEAAEKGILTFDKLEAMTAVCSVGLDMIAVPGDTPATTISGMIADEAAIGMINNKTTAVRVIPVPGKTVGEDIEFGGLFGRAPIMAVHEASSAAMVNRGGRIPAPVHSFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIH9</accession>
    <name>H4GIH9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0237 protein PS3_3760</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_3760</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0237 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121863.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000017.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3760"/>
    <dbReference type="EnsemblBacteria" id="EHS87300">
        <property type="protein sequence ID" value="EHS87300"/>
        <property type="gene ID" value="PS3_3760"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.380"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04872">
        <property type="entry name" value="ACT_1ZPV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01054">
        <property type="entry name" value="UPF0237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022986">
        <property type="entry name" value="UPF0237_ACT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="2">
        <location>
            <begin position="4"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="93" mass="10089" checksum="D3A1DD0518B89771" modified="2012-04-18" version="1">MKAIITTVGQDQVGIIAGVSNYLAKQHINILDVSQTIMDGFFTMMMLVDLAEDADFAALTEDLSQLGQELGVDINIRNEKVYAAMHELSGGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>H4GII5</accession>
    <name>H4GII5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_9000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000018">
        <property type="protein sequence ID" value="EHS87280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121869.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000018.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87280">
        <property type="protein sequence ID" value="EHS87280"/>
        <property type="gene ID" value="PS3_9000"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.387"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="201" mass="23138" checksum="FFB9CD96245B31E2" modified="2012-04-18" version="1">MDLSAIRQTLVINQQQGTLVEVYNFNDDDMFDVGFVVAIDELFVLLLGLDWDGKINGLTAIRLTSIHQVREQTDYLLTVSTKAKVAQNHGYFDIWHLQEYLRQHSFKQVNILRTLLEEAYQQHQAVVLGTDQYKGSDDFQGYLTDLSAINLTLHYYNSHDLSSLWEYEILIAKVDYLRVHGYQAAQTKAIFEEVFHEGSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GII6</accession>
    <name>H4GII6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LYZ2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_18559</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 73 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000018">
        <property type="protein sequence ID" value="EHS87281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18559"/>
    <dbReference type="EnsemblBacteria" id="EHS87281">
        <property type="protein sequence ID" value="EHS87281"/>
        <property type="gene ID" value="PS3_18559"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.388"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002901">
        <property type="entry name" value="MGlyc_endo_b_GlcNAc-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01832">
        <property type="entry name" value="Glucosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00047">
        <property type="entry name" value="LYZ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="LYZ2" evidence="4">
        <location>
            <begin position="129"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="56"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="285"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="288"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="339" mass="38706" checksum="50987BE774BA2034" modified="2012-04-18" version="1">MPASSIPFSDQSSSTQQQDQLTWFYEGIDDYLHDRKRDYHDERARQWYERGRQLAKDWQEEHPQVKEPTLNDIQPAQTEPTQVESVPQVIEAPSPIPASSANDIIPSEDITTSSSSEVQESDDTEVYPRLKAPLSHEQAEFIKTLSRNAVPIGNRYDLYPSIMMAQAILESNWCQSKLARQSHNLFGVKGDFQGNSVMMPTNEHLDGANQVINGQFKHYPNVEAALIDYAMVMDQPIYQNVHRSHSKNYQSAIKSLTGVYATDPHYHQKLDQLIRSYHLDKYDHDQQSSTANRYPATMTTNGHEKASSSPSKTSWNWQWPMLGGMGSVGVIGLIRRFWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIL1</accession>
    <name>H4GIL1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ</fullName>
            <ecNumber evidence="10">4.2.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase</fullName>
            <shortName evidence="10">(3R)-hydroxymyristoyl-ACP dehydrase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Beta-hydroxyacyl-ACP dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">fabZ</name>
        <name evidence="11" type="ORF">PS3_11870</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Involved in unsaturated fatty acids biosynthesis. Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:13097"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="4.2.1.59"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the thioester dehydratase family. FabZ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.59" evidence="10"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121895.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11870"/>
    <dbReference type="EnsemblBacteria" id="EHS87206">
        <property type="protein sequence ID" value="EHS87206"/>
        <property type="gene ID" value="PS3_11870"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.422"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008659">
        <property type="term" value="F:(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008693">
        <property type="term" value="F:3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047451">
        <property type="term" value="F:3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004317">
        <property type="term" value="F:3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00406">
        <property type="entry name" value="FabZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013114">
        <property type="entry name" value="FabA_FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010084">
        <property type="entry name" value="FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30272">
        <property type="entry name" value="PTHR30272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07977">
        <property type="entry name" value="FabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01750">
        <property type="entry name" value="fabZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="144" mass="15717" checksum="1677ABC4F83DC031" modified="2012-04-18" version="1">MSEPLLDSTAIQKILPHRYPMLLIDRVEELEPGQRAVATRNVTINENVFNGHFPQNPVLPGALIVESLAQTGAVALLSQPEFKGKTAYFGGIKSAQFRKVVRPGDVMKLEVTLDKVKRNIGSGKGIAWVDGKKACTAELTFFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIM1</accession>
    <name>H4GIM1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase M22, glycoprotease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_17470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121905.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17470"/>
    <dbReference type="EnsemblBacteria" id="EHS87216">
        <property type="protein sequence ID" value="EHS87216"/>
        <property type="gene ID" value="PS3_17470"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.435"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022496">
        <property type="entry name" value="T6A_TsaB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF11">
        <property type="entry name" value="PTHR11735:SF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03725">
        <property type="entry name" value="T6A_YeaZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TsaD" evidence="1">
        <location>
            <begin position="33"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="241" mass="26324" checksum="880EF80985B4183D" modified="2012-04-18" version="1">MKILAIDTSNQPMAIALVENRNLMATTTLKMVKNHSVYVLPTIDRLMEIVKWTPEDLDQIVVASGPGSYTGIRIAVTTAKVLADTLGINLVTVSSLQVLAANIPPLTNEIIVPFFDARRGTVFAGQYQWQNGSLLNIQSDQHLEMADLISQLSKCADSVVLVGEVTAKLAPLLTDLPQGIRLMTDVSALPSAYQLALLGDGKASVADVNTVVPRYSRRTEAEMNWLQTHPEGMDHDYVQRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIM4</accession>
    <name>H4GIM4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA small subunit methyltransferase I</fullName>
            <ecNumber evidence="6">2.1.1.198</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">16S rRNA 2'-O-ribose C1402 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">rRNA (cytidine-2'-O-)-methyltransferase RsmI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rsmI</name>
        <name evidence="8" type="ORF">PS3_13434</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine(1402) in 16S rRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(1402) in 16S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42924"/>
            <dbReference type="Rhea" id="RHEA-COMP:10285"/>
            <dbReference type="Rhea" id="RHEA-COMP:10286"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.198"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the methyltransferase superfamily. RsmI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.198" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121908.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13434"/>
    <dbReference type="EnsemblBacteria" id="EHS87219">
        <property type="protein sequence ID" value="EHS87219"/>
        <property type="gene ID" value="PS3_13434"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.438"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070677">
        <property type="term" value="F:rRNA (cytosine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000453">
        <property type="term" value="P:enzyme-directed rRNA 2'-O-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11648">
        <property type="entry name" value="RsmI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01877">
        <property type="entry name" value="16SrRNA_methyltr_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008189">
        <property type="entry name" value="rRNA_ssu_MeTfrase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018063">
        <property type="entry name" value="SAM_MeTrfase_RsmI_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46111">
        <property type="entry name" value="PTHR46111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005917">
        <property type="entry name" value="MTase_YraL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00096">
        <property type="entry name" value="TIGR00096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01296">
        <property type="entry name" value="RSMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="7">
        <location>
            <begin position="14"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="287" mass="32128" checksum="AC5D4A5CACABDD1D" modified="2012-04-18" version="1">MQAVSSFQESINGRLFLVPTPIGNLDDMTFRAIKVLREVDLIAAEDTRHTRQLLNHFEIDTPMISFHEHNTEQRIPQLLERLTQGESIAQCSDAGMPSISDPGKELVAACVQANIPVIPLPGANAGLTALIASGLVPQPFYFYGFLERKTSLQQESLAALKERQETIIFYEAPHRLAKTLQNMVTVIGPERPVVLARELTKRYEEFIRGSLAEVLAWAQTDQVRGEFVILLAGHPGDDDKSSEITENPIDQIKHLIDQGEKVNTAIKLVAKKRGLNRQALYREFHNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIN7</accession>
    <name>H4GIN7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycogen synthase</fullName>
            <ecNumber evidence="7">2.4.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Starch [bacterial glycogen] synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">glgA</name>
        <name evidence="10" type="ORF">PS3_834</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Synthesizes alpha-1,4-glucan chains using ADP-glucose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + ADP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18189"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.21" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121921.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_834"/>
    <dbReference type="EnsemblBacteria" id="EHS87232">
        <property type="protein sequence ID" value="EHS87232"/>
        <property type="gene ID" value="PS3_834"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.453"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033201">
        <property type="term" value="F:alpha-1,4-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004373">
        <property type="term" value="F:glycogen (starch) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009011">
        <property type="term" value="F:starch synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00484">
        <property type="entry name" value="Glycogen_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011835">
        <property type="entry name" value="GS/SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02095">
        <property type="entry name" value="glgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_transf_5" evidence="9">
        <location>
            <begin position="2"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="8">
        <location>
            <begin position="290"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>ADP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="476" mass="54683" checksum="D4B5948F489AFBB4" modified="2012-04-18" version="1">MRVLFSGAECAPFIKTGGLGDVLGALPNQLAKDGDEVGVILPMYRDIPQTYQEKFTYLGNFYVEVGWRNQYCGIFEYQDRGVRYFFVDNQYYFDRPVVYGYYDDGERFAFFQQAVIMFMERFDFIPNILHCNDYHTAFIPFLLREKWGFVGAYAQIKTVFTIHNIQFQGQYDANVLPELFGLGYDWYDNGTVRQDNDVNWMKTGILYADRVTTVSPTYAEEIKTSEFGYGLDSVLRSVAWKVTGILNGIDNQKFNPQTDPDIPVHYSVKKLAGKKKDKSALQKELNLPIKARVPMIGLVSRLTAQKGCQLLVEELDNILQFNVQVVLLGNGDQFFEDRFKEIASRHPDKFRLMLAFDPQLAQRIYAGCDAFLMPSAFEPCGLSQLIALRYGTLPIVHQIGGLADTVWAYNPETNEGTGFGFRDYQGYVMVDKIKQMLDVYAKPQVWQKMMHTAMKSDFSWKDSASKYQWMYGELIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIN9</accession>
    <name>H4GIN9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">1,4-alpha-glucan branching enzyme GlgB</fullName>
            <ecNumber evidence="10">2.4.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Alpha-(1-&gt;4)-glucan branching enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Glycogen branching enzyme</fullName>
            <shortName evidence="10">BE</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">glgB</name>
        <name evidence="13" type="ORF">PS3_16963</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Transfers a segment of a (1-&gt;4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.</text>
            <dbReference type="EC" id="2.4.1.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.18" evidence="10"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16963"/>
    <dbReference type="EnsemblBacteria" id="EHS87234">
        <property type="protein sequence ID" value="EHS87234"/>
        <property type="gene ID" value="PS3_16963"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.457"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003844">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102752">
        <property type="term" value="F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02855">
        <property type="entry name" value="E_set_GBE_prok_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00685">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006048">
        <property type="entry name" value="A-amylase/branching_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037439">
        <property type="entry name" value="Branching_enzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006407">
        <property type="entry name" value="GlgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044143">
        <property type="entry name" value="GlgB_N_E_set_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004193">
        <property type="entry name" value="Glyco_hydro_13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43651">
        <property type="entry name" value="PTHR43651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02806">
        <property type="entry name" value="Alpha-amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02922">
        <property type="entry name" value="CBM_48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000463">
        <property type="entry name" value="GlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01515">
        <property type="entry name" value="branching_enzym"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 10" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="6 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aamy" evidence="12">
        <location>
            <begin position="140"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10 11">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10 11">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000463-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS87234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="629" mass="73493" checksum="61BD06BDD2EFD2B7" modified="2012-04-18" version="1">MKERLAYFTKGEEYHLQDFMGCHRTDDGFVFRVWAPHAKQVWAVGDFNDWQKTHPMVKDDYGIWSITIGRVQTGQLYKFLVQEPNGKEVMKIDPMALRYEARPGTAAMVTEMPTRKWHDGLWKGRNKRSNNFSRPMNIYEVHPASWKQHADGSLYQFKDLTAELIPYVKEQGFNYIEFMPLTEHPLDASWGYQVTGYFALAHAYGEMADFQEFVDACHQANIGVLIDWVPGHFCINADALAYYDGTPTYEYQEKWRADNRGWGALNFDLGKPEVVSFLLSSALFWIENYHVDGLRVDAVSNMLYRDYDRGPGEWEPDQYGGNRNLEGIAFLHTLNRTIKWMHPEVLMIAEESSSQVKITGRIEDGGLGFDYKWNMGWMNDQLDFYGMDPYFRKDNFNQATFSFMYRMSENFILPLSHDEVVHGKRSLMNKMWGDRHQQFDQLRLLLTMQMTYPGKKLLFMGGEYGQYLEWRFNEGLEWSNQTDPLNDQMLAFDRSLNHFYLQERALWELEQEEASLVVIDADNRDESVLSFIRQGKTRHDFVVVILNFTPVERDHFTIGVPYAGTYQEVFNSALKAYGGTWKTDNPESQTVARPFKQFDYQLETNVPAFGALILKPTKVTIKPHRHKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIP9</accession>
    <name>H4GIP9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_8652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8652"/>
    <dbReference type="EnsemblBacteria" id="EHS87244">
        <property type="protein sequence ID" value="EHS87244"/>
        <property type="gene ID" value="PS3_8652"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.469"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045509">
        <property type="entry name" value="HD_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19276">
        <property type="entry name" value="HD_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HDc" evidence="1">
        <location>
            <begin position="57"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="448" mass="51975" checksum="830E1C95C072FC64" modified="2012-04-18" version="1">MSFQDENLQKEKVFRDPIHGTIIVDNQIILDLINTPELQRLRRIKQLGTTSLIFHGAEHSRFGHSLGAYEIARRMCNHFQRNYPQLTPGDGRWDDQERSVTLCAALLHDLGHGPYSHTFEHIFNTNHETITRQIITQDTNVNRILRRVSDQFPEQVASVIDHTYPNPQVVQMISSQVDVDRMDYLQRDAYYTGTNYGLVDLDRVLQVMRPIKDGIAFDISGMHAIEDYIISRLQMYLQVYFHPVTRSLEVILDHLLMRVKHLYEDRPSLSEFTPDLLMPFFNQEPQLDDYLALDDGVLTTYFQHWTHAHDDILADLANRFLNRVPLKSVIYDQSTANLIPQLQDLITKAGFNSKYYTDTNSSYKLPYDTYNPKEANPQSQIELLQPNGELIELSSVSTLVHSVSGHAQGDERFFFPKEMLGETDQTELFAPIYQEFQSHIQNHTIIKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIU8</accession>
    <name>H4GIU8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">50S ribosomal protein L14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rplN</name>
        <name evidence="8" type="ORF">PS3_13754</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L19. In the 70S ribosome, L14 and L19 interact and together make contacts with the 16S rRNA in bridges B5 and B8.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121981.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13754"/>
    <dbReference type="EnsemblBacteria" id="EHS87178">
        <property type="protein sequence ID" value="EHS87178"/>
        <property type="gene ID" value="PS3_13754"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.530"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_L14_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_L14P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005745">
        <property type="entry name" value="Ribosomal_L14P_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_L14P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="PTHR11761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="SSF50193"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01067">
        <property type="entry name" value="rplN_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 5 7" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="122" mass="13161" checksum="3253A0FBB3E51273" modified="2012-04-18" version="1">MIQQESRLKVADNSGARELLVIKILGGSRVKTGNIGDVIVATVKQATPGGVVKKGDVVKAVVVRTKHGLHRADGSYIKFDENAAVLINNDKSPKGTRIFGPIARELRDDDYMKIISLAPEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ09</accession>
    <name>H4GJ09_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NCS2 family nucleobase:cation symporter-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_14492</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122042.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14492"/>
    <dbReference type="EnsemblBacteria" id="EHS87121">
        <property type="protein sequence ID" value="EHS87121"/>
        <property type="gene ID" value="PS3_14492"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.626"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017588">
        <property type="entry name" value="UacT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006042">
        <property type="entry name" value="Xan_ur_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00801">
        <property type="entry name" value="ncs2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03173">
        <property type="entry name" value="pbuX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01116">
        <property type="entry name" value="XANTH_URACIL_PERMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="233"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="397"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="427" mass="45608" checksum="06F066187390DEF0" modified="2012-04-18" version="1">MPSKEVSHQKAAVLGIQHLLAMYSGDVAVPLLIGHALNFNADQMTYLVSIDIFMCGLATFIQLIRNRYFGIGLPVVLGCAIQAVQPLEMIGKKLSIGTMYGAIIVAGLFVFLIAGYFAKLRRFFPPVVTGTLITVIGLTLIPVGIQDIGGGDATAKSFGDWHNLLLGLITVLIIIAVQIFTRGFISSIAVLIGLVVGSLIAALMGMVSTDSVAQAAWFHVPTPFYFGLPNFEWSSIVTMIIVSLVSMVESTGVFFAIGDLLHRDVSADDLKRGYRAEGLAVMLGGIFNTFPYTTFSQNVGLLQLSGVKTKRPIYWSACLLMILGLLPKIGALVTMIPTPVLGGAMLVMFSMISVQGIRMLIQVDFGDQRNILIVAISIGLGLGVSVYPTLFQGLPTTVQLLLGNGIVIASIVAVFLNVALKGRDGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ15</accession>
    <name>H4GJ15_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_16201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000027">
        <property type="protein sequence ID" value="EHS87091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87091">
        <property type="protein sequence ID" value="EHS87091"/>
        <property type="gene ID" value="PS3_16201"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.635"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010315">
        <property type="entry name" value="DUF915_hydro-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06028">
        <property type="entry name" value="DUF915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="268" mass="29772" checksum="9218C710381CF924" modified="2012-04-18" version="1">MIVGFGWVKTSKAQRYVQSTTPTLFFHGGGSSYHAEEHMVNAAKRAGVTNTVIRANVDPNGKVTLSGSMTKHSINPIVEVNYENNRQLDYSQHGRWAANVVKLLQKRYGIKQVKMVGHSLGNISIIYYELQNAQKKSMPKVVKQVDIAGHFAGLNFSQVPASIRQPAGLKLDSKTGKPNQMNATYQEMTKLRHVLPKNQVQVLNIYGDIGGQTDGTVPNVSSLSLKYLIASRAKSYRELKITGKQARHSKLHSNAKVDRALINFLWSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ27</accession>
    <name>H4GJ27_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_4783</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122060.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4783"/>
    <dbReference type="EnsemblBacteria" id="EHS87052">
        <property type="protein sequence ID" value="EHS87052"/>
        <property type="gene ID" value="PS3_4783"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.660"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021247">
        <property type="entry name" value="DUF2785"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10978">
        <property type="entry name" value="DUF2785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="324" mass="37636" checksum="C461E0453772AFC6" modified="2012-04-18" version="1">MDEEQIKHLNQQVRELRHATANGQIFKQLDRLAGDLIEQTPRHPRTPIATVEDDAAFYDELRQMYKKLQDDQLDQVSDVWLLSLLDHFKVDDPAIRLNGCLMIFNALLANDLLTNDQLRLSFHYLAQNDILLAHIDEPKNRAVFGRATALSALASLLYADRAGYFFLQEYEVDRLVDLVSIILVMEKDTRGFVGEQGWAHMYAELANVLGELSQREELVRGDKVSLMALLLANYSRLTTPLIMGENEQLADYLVNLMNQHQVYRRYFLLALRNWRLRLRAGRPQNAGNWHQIFNYRRLMQCLLMVNDLPEQIGKVILEQDDQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ34</accession>
    <name>H4GJ34_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP synthase subunit b</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP synthase F(0) sector subunit b</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">ATPase subunit I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">F-type ATPase subunit b</fullName>
            <shortName evidence="11">F-ATPase subunit b</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">atpF</name>
        <name evidence="14" type="ORF">PS3_62</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0).</text>
    </comment>
    <comment type="function">
        <text evidence="10 11">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the ATPase B chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122067.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_62"/>
    <dbReference type="EnsemblBacteria" id="EHS87059">
        <property type="protein sequence ID" value="EHS87059"/>
        <property type="gene ID" value="PS3_62"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.671"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01398">
        <property type="entry name" value="ATP_synth_b_bprime"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028987">
        <property type="entry name" value="ATP_synth_B-like_membr_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002146">
        <property type="entry name" value="ATP_synth_b/b'su_bac/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005864">
        <property type="entry name" value="ATP_synth_F0_bsu_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00430">
        <property type="entry name" value="ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81573">
        <property type="entry name" value="SSF81573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01144">
        <property type="entry name" value="ATP_synt_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 11 12" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5 11 12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 11 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 11 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="84"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS87059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="169" mass="18925" checksum="1BD3C16A18BF1990" modified="2012-04-18" version="1">MFVLAESNSLYVGDQIFYLVSFIILALLVWHFAWKPVTQMMQKRADKIANDIDTAQQSHEEAEKLAAQRQAELKGTKAEAAKIVDDAKKSGESQRTQIVETAQQDAQNLKEQARRDADQARENALRGAKDDVANLSIEIASKLIQKQLNADDQQALIDSYIEGLVKHES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ74</accession>
    <name>H4GJ74_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Exopolyphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_21010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21010"/>
    <dbReference type="EnsemblBacteria" id="EHS87029">
        <property type="protein sequence ID" value="EHS87029"/>
        <property type="gene ID" value="PS3_21010"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.723"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="507" mass="58370" checksum="76DEAD6C609EDE73" modified="2012-04-18" version="1">MGENTMAGKTYGLIYMSPIDIRLDIVNLKKQTVIDRVNSVPFVSVGNYQAEMPQIIETLMGFKQLMADYGVADYHFWWHYQTVDPITAGYICDQIKIRTDLDVDLLNLGQVTYLKNSAVLASLKQGDLQLTTDYVYQLTLSPQGIVISEFYRHEFIQSWTISLNLNEFKLISDSMSHSSLNPIEIMIDYTRSKLEYLRKELVHSDLATLIIQDAWILNRHFLAPGEEFKLLSRDELKQCFTDATQQSDQYLMQHYGFEEEQGARFLFGMIVIQRVFHYIAPRETYLTNRSIGHGLVLNEASQAGYLKDNYAEVILTAAKNIAERYQVDLHHANQVHRFAKHLFEQLKKLHRLDKKEELLLEVACYTDDIGTFINQSGHYDHSAYILQANPIIGLSDEENKIIAEISRYHSSESPETGQAHYRHLDNDIQMIVAKLASILRLADALDDSRRQKIDKISLSLKEGELIVTVSSNQNLALERWSFDLKASLFKEVFGVKPVFKQRRVKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJA5</accession>
    <name>H4GJA5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prophage Lp2 protein 18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_18882</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000031">
        <property type="protein sequence ID" value="EHS87017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122138.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000031.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87017">
        <property type="protein sequence ID" value="EHS87017"/>
        <property type="gene ID" value="PS3_18882"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.795"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007731">
        <property type="entry name" value="DUF669"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05037">
        <property type="entry name" value="DUF669"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="244" mass="26752" checksum="8DEF8EE83650C906" modified="2012-04-18" version="1">MLFTTNSNDVFSGARVEEAGNYNVKVLSTSTLTKTKANGNPMITLNYEVIDGKYQGGQIRYDNQTWVDYDQESHAQSVKRFNTLLKATGLQDGVQVDTMQQLLNLLVGKTMSIKVEWEQGNNGYYNLRVKQHNSVLQDGSKPNGQKSPDTGTDNFNSGNGSYSGNFGGGNFNNPANSPQNGPQGNLNNQWGNNQSSNQSGLNGKNWANNDPLAGQPQLFKDLFKDQPIDTDSLPFGDQVKDAPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJB6</accession>
    <name>H4GJB6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_8072</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000032">
        <property type="protein sequence ID" value="EHS87006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122149.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000032.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8072"/>
    <dbReference type="EnsemblBacteria" id="EHS87006">
        <property type="protein sequence ID" value="EHS87006"/>
        <property type="gene ID" value="PS3_8072"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.813"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="PTHR43827"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="4">
        <location>
            <begin position="21"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="3">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="285" mass="32098" checksum="6C9D7A943A7C928F" modified="2012-04-18" version="1">MQTVKLSNGLEMPQLGFGVFQIEPDKTKDVVLEAIKVGYRSFDTATAYQNEAQLGQAIQASGIDRAEFFITSKLWVSDANYERAKAGIQASLERLGTDYIDLYLLHQPFGDVYGAWRALTEAYEAGQLKAIGVANFEPDHLKNLELMSKVKPMVNQIEINPFYTEECEVKFNQAEGVQVMSWAPFAEGHNGIFTNETLQAIADAHHKTVGQVILRWELQQGIILIPKSVHQERMQENFAIFDFELSDEEMKQVASLNTGKSQFFSHWDPVAIEQIFGSSLAQLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJC7</accession>
    <name>H4GJC7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_12071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000033">
        <property type="protein sequence ID" value="EHS86996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122160.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000033.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS86996">
        <property type="protein sequence ID" value="EHS86996"/>
        <property type="gene ID" value="PS3_12071"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.829"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007563">
        <property type="entry name" value="DUF554"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36111">
        <property type="entry name" value="PTHR36111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04474">
        <property type="entry name" value="DUF554"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="223" mass="24438" checksum="2FA530B62AC9DC75" modified="2012-04-18" version="1">MIGIWANVIAIIVGTLIGWLFKRRLSDKYIDALWQSLGLLALGVGIQTVVNNMNKSHYPLLFIFSLAIGIPLGTFFNLNDRATGWIDRHFQSHLGEGVATASFLDGIGALAILGPVTAATNGNQTMLYTNAMLTLVCGIIFGTSFGLGMILESPIIFIWFSFIYFVTKYLSASYFSNSLVVEMSVVGGFLVMAGGLSLLKIREFKTVDMLPSLLVPMIYFLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJD1</accession>
    <name>H4GJD1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Glutamate racemase</fullName>
            <ecNumber evidence="2 7">5.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">murI</name>
        <name evidence="8" type="ORF">PS3_1421</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Provides the (R)-glutamate required for cell wall biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>L-glutamate = D-glutamate</text>
            <dbReference type="Rhea" id="RHEA:12813"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29986"/>
            <dbReference type="EC" id="5.1.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the aspartate/glutamate racemases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.3" evidence="2 7"/>
    <dbReference type="EMBL" id="AICN01000034">
        <property type="protein sequence ID" value="EHS86972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122164.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000034.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_1421"/>
    <dbReference type="EnsemblBacteria" id="EHS86972">
        <property type="protein sequence ID" value="EHS86972"/>
        <property type="gene ID" value="PS3_1421"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.835"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008881">
        <property type="term" value="F:glutamate racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00258">
        <property type="entry name" value="Glu_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015942">
        <property type="entry name" value="Asp/Glu/hydantoin_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001920">
        <property type="entry name" value="Asp/Glu_race"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018187">
        <property type="entry name" value="Asp/Glu_racemase_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033134">
        <property type="entry name" value="Asp/Glu_racemase_AS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004391">
        <property type="entry name" value="Glu_race"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21198:SF2">
        <property type="entry name" value="PTHR21198:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01177">
        <property type="entry name" value="Asp_Glu_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53681">
        <property type="entry name" value="SSF53681"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00067">
        <property type="entry name" value="glut_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00923">
        <property type="entry name" value="ASP_GLU_RACEMASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00924">
        <property type="entry name" value="ASP_GLU_RACEMASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="10"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="42"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="74"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="185"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="265" mass="28898" checksum="9D1CC7503B20CBC1" modified="2012-04-18" version="1">MDNRPIGVMDSGIGGLSIVRVLMQELPHESIIFVGDQLHFPYGTRTTENIRDLALKISQYLVAQNVKLLVIACNTATAAALPILQAQLPIPVIGVIEPGARAAIEVAQTPRIGVIATDSTIRSQAYSQALHRLVPETNVVSQATQPLVSVVEHGLTGTFQAQQMVNNALAIFKEQPVSALILGCTHFPFLEYEIHHCLGKEVVLIDPAEETVKMVHSQLAQNDQLADQIDPQSTFYSTGDQLELLAGVNRWVNPQAKYQCAHLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJD5</accession>
    <name>H4GJD5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exodeoxyribonuclease 7 large subunit</fullName>
            <ecNumber evidence="5">3.1.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Exodeoxyribonuclease VII large subunit</fullName>
            <shortName evidence="5">Exonuclease VII large subunit</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">xseA</name>
        <name evidence="10" type="ORF">PS3_12916</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterooligomer composed of large and small subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the XseA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.6" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000035">
        <property type="protein sequence ID" value="EHS86884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122168.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000035.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12916"/>
    <dbReference type="EnsemblBacteria" id="EHS86884">
        <property type="protein sequence ID" value="EHS86884"/>
        <property type="gene ID" value="PS3_12916"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.844"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009318">
        <property type="term" value="C:exodeoxyribonuclease VII complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008855">
        <property type="term" value="F:exodeoxyribonuclease VII activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04489">
        <property type="entry name" value="ExoVII_LU_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00378">
        <property type="entry name" value="Exonuc_7_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003753">
        <property type="entry name" value="Exonuc_VII_L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020579">
        <property type="entry name" value="Exonuc_VII_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025824">
        <property type="entry name" value="OB-fold_nuc-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30008">
        <property type="entry name" value="PTHR30008"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02601">
        <property type="entry name" value="Exonuc_VII_L"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13742">
        <property type="entry name" value="tRNA_anti_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00237">
        <property type="entry name" value="xseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA_anti_2" evidence="9">
        <location>
            <begin position="8"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Exonuc_VII_L" evidence="8">
        <location>
            <begin position="137"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Exonuc_VII_L" evidence="8">
        <location>
            <begin position="358"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS86884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="507" mass="57563" checksum="6838FAB48C22A3AF" modified="2012-04-18" version="1">MINEQECLTVAQITKYIKTKFDVDPYLNQKVLLVGQLTDFRQRSGHQYFSLKDDDDKIANPAKIRIVMFKSAFQKVKFQPENGMKVLLQGRISIYEPTGSYQFYAEQMEVAGVGSLQIAFEQMYKKLKAEGLFDRPHRSIPPFPKQIAVVTSPNGAVMHDIITTMRRRNRLVQLIFYPTRVQGETAANEIATQIEHVNQDGGYDALIIARGGGSLEDLWPFNEEVVGRSIAASTIPVISSIGHETDTTIADLAADVRAATPTAAAEIASAWELSDVVATVDELQIQLYRQMKNRLQDLKRYVASLVDNSILQTPDRLLARPMQQVDELLHQLTTSLNSELQVKERQYQQMRDRLISQSPTKKLARQNLLRVQVSQQLIHAYQSQLRAKQALLTNTSQQLRFAYQAQLNRQRQSLKNLNQQVMTISRQLISEENYRLKGLEQVTDALIEQLDSLSPLNLLKRGYTYVTDEQQTVITGMDQLELGQTVELHFANGKALAEIKNKEDEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJD9</accession>
    <name>H4GJD9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative Xaa-Pro dipeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_7959</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M24B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000035">
        <property type="protein sequence ID" value="EHS86888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122172.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000035.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7959"/>
    <dbReference type="EnsemblBacteria" id="EHS86888">
        <property type="protein sequence ID" value="EHS86888"/>
        <property type="gene ID" value="PS3_7959"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.848"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_NTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="SSF53092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase_N" evidence="5">
        <location>
            <begin position="3"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M24" evidence="4">
        <location>
            <begin position="136"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS86888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="360" mass="40138" checksum="5728B7E0B08A2826" modified="2012-04-18" version="1">MTRLERLRKQFVKQYIDSLIIMDPANIYYLTGIELMAGDGLLFVTNDLAVIITDDRYETYLDELENDQVMGLITRDYFGKLAELVGKLEVEIVGFENTLSYRDYESLDDLMPVGMVACDRIVERLRRIKDPEELNKLRQSAHVHDLGYQYVLSIIKPGMTEKKVADQLDAFMKQHGASEASFPTILASGPNSAKPHATVSDRPIQVGDVVTLDFGYFVDHYTADMTRTFVIGQATPEIEKVYQLVNQARQAVIDHMKAGMAGNLADQAGRSLIDLAGYGQYFNHGMGHGIGLAVHEFPASYGPKTKKLKLRHQEILTVEPGIYLPGKFGVRIEDDVIVSHAGVEVITKTPTDLVVVTPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJE6</accession>
    <name>H4GJE6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase S74 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_20203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000036">
        <property type="protein sequence ID" value="EHS86869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20203"/>
    <dbReference type="EnsemblBacteria" id="EHS86869">
        <property type="protein sequence ID" value="EHS86869"/>
        <property type="gene ID" value="PS3_20203"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.869"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030392">
        <property type="entry name" value="S74_ICA"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51688">
        <property type="entry name" value="ICA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S74" evidence="1">
        <location>
            <begin position="206"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="308" mass="33346" checksum="37C0EB79344273BA" modified="2012-04-18" version="1">MSADKITSGTIATGTLKVNLNQGLIDFRTGNGSGTYFTKDQVVFSKNNVEPGFIGIAGSPIGNTTTNELSINYHGKNKGGFAVRDIDNLDRQGRLIVSNEDNRWETHTNNATYSKLVVGNKDPYFEYHYGVGLKTDVINGLYIDQSGASLKYKSVSQGTGTGINIHDALINVHGDDTTSAVGCQLRVQGWSWVAGYVEAMGHSLHSALSLKTNIERLDPAYCLALINQTDLTSFTFKAEVASGSNFRHFGPIIDDVNQVAQYNTPPEFIAATYGDAPARDDDNIIGALMGAVQELTKRLEKVEKTNGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJE7</accession>
    <name>H4GJE7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NlpC/P60 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_18545</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C40 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000036">
        <property type="protein sequence ID" value="EHS86870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18545"/>
    <dbReference type="EnsemblBacteria" id="EHS86870">
        <property type="protein sequence ID" value="EHS86870"/>
        <property type="gene ID" value="PS3_18545"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.870"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000064">
        <property type="entry name" value="NLP_P60_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010572">
        <property type="entry name" value="Prophage_Tail"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00877">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06605">
        <property type="entry name" value="Prophage_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51935">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NlpC/P60" evidence="2">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="298" mass="32626" checksum="40CD31408277CD67" modified="2012-04-18" version="1">MGAKKWISVAKSYVGIDYVYGGGHSASGDPKAGMDCSGLGEKIAQACGLDIGWGATTTLESQGNVINRSEVQTGDMGFYGSRGSSYHVVWALDNRQFIAEPQPGQKCYIGNIDGYPADFWIRNPKIAAFVGNGTTSGSGGSGESTVTVYYFTPFYYINQESAKRWGVHETDDITSDSISTVEEMKKYADKTFSLNPEFNLTATLDPNTEIVKGDITHVSIRPADYETDLKLVGYTQYPYSSAQQPSVTYNSNPATILDFYTSQEKKISSVQRQLVDATKISRTNQNWLNNYVGGETNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJF2</accession>
    <name>H4GJF2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP-dependent Clp protease proteolytic subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_9161</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase S14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000036">
        <property type="protein sequence ID" value="EHS86875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9161"/>
    <dbReference type="EnsemblBacteria" id="EHS86875">
        <property type="protein sequence ID" value="EHS86875"/>
        <property type="gene ID" value="PS3_9161"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.880"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="PTHR10381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="253" mass="28282" checksum="85B9ECC169FE869D" modified="2012-04-18" version="1">MTTMPKWLTIKQLTDKQADMFIGGEIVTDEWDDSDTSASGFRDALQSVGDVKTINLHINSPGGSVFEGIAIYNMLKQHQATINVYVDGLAASIASVVAMAGNRIYMPQNAMLMIHNPWTMAVGNATELRKQADDLDQIAKSSITTYLSKAGDKLDEETLKQMMDEETWLTAEEALEYGLADNVMEPNKMAASISDKYKARYHNVPDQLIMNSSEKVDKRKNGLTDDQRQTLIEETLNRRELVTTQLKQLQGEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJF3</accession>
    <name>H4GJF3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Prophage L54a, terminase, large subunit, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_13705</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000036">
        <property type="protein sequence ID" value="EHS86876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13705"/>
    <dbReference type="EnsemblBacteria" id="EHS86876">
        <property type="protein sequence ID" value="EHS86876"/>
        <property type="gene ID" value="PS3_13705"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.884"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005021">
        <property type="entry name" value="Terminase_largesu_Lambdalike"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41287">
        <property type="entry name" value="PTHR41287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03354">
        <property type="entry name" value="Terminase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="145" mass="16990" checksum="668967FDC7A450C9" modified="2012-04-18" version="1">MDYAGIVEWAKSYIKNEKEQAHILDNPSPVLLTTYYAQAVVEGSVMASKWVKLACERHLKDLEKSKNDPDYPWAFDEEKAHRPIRFIEKKCKPSKGDYDHLVLQPWQHFFVGNIFGWVNREAGYRRYREALVFLGRKNGKVISPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJH2</accession>
    <name>H4GJH2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Elongation factor P</fullName>
            <shortName evidence="8">EF-P</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">efp</name>
        <name evidence="12" type="ORF">PS3_14226</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 9">Belongs to the elongation factor P family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000039">
        <property type="protein sequence ID" value="EHS86615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122205.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000039.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14226"/>
    <dbReference type="EnsemblBacteria" id="EHS86615">
        <property type="protein sequence ID" value="EHS86615"/>
        <property type="gene ID" value="PS3_14226"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.923"/>
    <dbReference type="UniPathway" id="UPA00345"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04470">
        <property type="entry name" value="S1_EF-P_repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05794">
        <property type="entry name" value="S1_EF-P_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00141">
        <property type="entry name" value="EF_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015365">
        <property type="entry name" value="Elong-fact-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020599">
        <property type="entry name" value="Transl_elong_fac_P/YeiP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013185">
        <property type="entry name" value="Transl_elong_KOW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001059">
        <property type="entry name" value="Transl_elong_P/YeiP_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013852">
        <property type="entry name" value="Transl_elong_P/YeiP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011768">
        <property type="entry name" value="Transl_elongation_fac_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30053">
        <property type="entry name" value="PTHR30053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01132">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08207">
        <property type="entry name" value="EFP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09285">
        <property type="entry name" value="Elong-fact-P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005901">
        <property type="entry name" value="EF-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01185">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00841">
        <property type="entry name" value="Elong-fact-P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00038">
        <property type="entry name" value="efp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01275">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 12" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EFP" evidence="11">
        <location>
            <begin position="67"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Elong-fact-P_C" evidence="10">
        <location>
            <begin position="129"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS86615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="185" mass="20476" checksum="03239AE4DEB1ACBE" modified="2012-04-18" version="1">MIQTIDLKKGMVFEKDGKLLKVLSINHHKPGKGNTLMQMDIQDLRSGSIVHTTMRPSEKVEQVNVDKRNAQYLYDEGNSSVFMDLETYEQYEIDGAQIQEEKKFMVENMQVQMDFVGSELVGVELPVTVTLTVADTQPMVKGATIDGGGKPATMNTGVVVQVPSFIKNGDEVIVNTTDGSYKSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJI2</accession>
    <name>H4GJI2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">FeS assembly ATPase SufC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_9144</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. Ycf16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000041">
        <property type="protein sequence ID" value="EHS86572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122215.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000041.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9144"/>
    <dbReference type="EnsemblBacteria" id="EHS86572">
        <property type="protein sequence ID" value="EHS86572"/>
        <property type="gene ID" value="PS3_9144"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.941"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd03217">
        <property type="entry name" value="ABC_FeS_Assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010230">
        <property type="entry name" value="FeS-cluster_ATPase_SufC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204">
        <property type="entry name" value="PTHR43204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01978">
        <property type="entry name" value="sufC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="4"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS86572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="264" mass="29247" checksum="666469219DE60924" modified="2012-04-18" version="1">MSTLEIKDLHVNVVDNEGKDEKEILKGVNLKMKTGEIHAIMGPNGTGKSTLSQTIMGQSGYRITQGDILLDGVSIVDMPVDERARRGLFLAMQYPVEIQGVTNTEFLRAAINARRPEDDPIPVMEFINKLDEKMELLDMPESVADRYLNEGFSGGEKKRNEILQLLMIEPQFAILDEIDSGLDIDALKVVSKGVNSMRGENFGSLIITHYQRLLNYIVPDTVHVMMGGKVVKTGGPDLAEKLEAEGYAGLRDELGLDIKLTDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJJ6</accession>
    <name>H4GJJ6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_10162</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122229.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10162"/>
    <dbReference type="EnsemblBacteria" id="EHS86506">
        <property type="protein sequence ID" value="EHS86506"/>
        <property type="gene ID" value="PS3_10162"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.965"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="204"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS86506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="415" mass="46852" checksum="3AF4571215650465" modified="2012-04-18" version="1">MIQRFRRLFIFISSTSLLFVLATIMTAVIGVSVIQTNRQVDSIMDILVQHDGQLDHREATQAAKKSLGSNSTAINILQYRYFTIFYNKSGSVKNVDLTNSPSLTSEDATYLAKLVKKDKNTSGTIDSDNAIYAYRIIKKSDGTREIIFLDKTTLNNATLAISKIGLCIAVIGWALFTVVLILFSKRAIRPIITAEKRQKQFITNAGHELKTPLAIISANNEMAEMLDGESEWTKNNKQQISRLTRLINNLINLARLDEDPEFDFQKIDASANVQRAVDSFKPLVENEDKQFNYQISKGIQLVVDENYFYELCNILLDNAVKYCDPHGTITVVLKKEGRNATLEIANTYAAGKGVDYSRFFDRFYRDNEAHTTKGQSGFGIGLSMARNLVTIFNGRINVTYRKDQIVFVTKFKLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJL3</accession>
    <name>H4GJL3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_4567</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122246.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4567"/>
    <dbReference type="EnsemblBacteria" id="EHS86523">
        <property type="protein sequence ID" value="EHS86523"/>
        <property type="gene ID" value="PS3_4567"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.991"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="299"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="353"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="408"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="437"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS86523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="464" mass="49964" checksum="A1045E73A6332513" modified="2012-04-18" version="1">MSQNIFRKESLATYLGSDKQFVKSMNGIDLMTLGIGAIIGTGIFILPGTVAATTAGPGVTLSFFFAAIVCALAAMCYAEFASALPVAGSAYSYGNVVFGEIVGWVLGWALILEYMLAVASVSTGWSAYFASFIESFGIKIPTALSGPFDPAHGTYINIVAVLIVLFVGWILSHGISTSIKINDVMVLVKLAIIVIFIVIGIFFIKPHNYHPFLPYHLFGSLKGATTVFFAFLGFDVVAASSAEVKNPQKNMPIGIIGSLIVATVLYMGVSIVLTGMVKYTHLDVANPVAFALKYVNQGWLADLLSLGALVGMFTMMISMTYSSSRLVYSIGRDGLLPNFISKFDEKHQAPQRALWIVTIIIAFMGGFVSLDQLTNLVNIGTLLAFTAVSFGVIPLRKRSDIKNDGFKVPLYPALPIISGLACIGMICLLSAETLINAAIWFVLGLIIYFAYGYRHSKLSHLSDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJP4</accession>
    <name>H4GJP4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glutamine ABC transporter permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_6141</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000045">
        <property type="protein sequence ID" value="EHS86245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6141"/>
    <dbReference type="EnsemblBacteria" id="EHS86245">
        <property type="protein sequence ID" value="EHS86245"/>
        <property type="gene ID" value="PS3_6141"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1033"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043429">
        <property type="entry name" value="ArtM/GltK/GlnP/TcyL/YhdX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614">
        <property type="entry name" value="PTHR30614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="441"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="271"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS86245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="468" mass="51195" checksum="C890E01E3A1EB143" modified="2012-04-18" version="1">MLGLVSFNSATPAHADEETYTIGTVDSFVPFEIPNNKGTYNGKNPGIEIEMLQQIAKHEHFKYNLRVMTFDAMLQALDANQIQAAFGGMSVTDERKAKIDFTTSYYNSGVVMAVAKNSKITSLSQLRGKTVDLKSGTSGAEYATSIQKKYGFKVKYFKSDNTLFSDVVNGNGAATFNDEPVLRYGIRNGMKLKIVTKAANSTPVAMGVKKGENQELLTKLNDGIAWLKKTGRMQKIINKYTKGSATKTDSASQRTIWALIKNNAGALADGLWMTIELTVVGSICAMVFGLLLGVMGITDGKLWSSISSIIIYIFRGIPMIVLAFFIYIGLPAVIGQKIPLFVAGVLTLMLDEGAYVGAIVKGGFQAVDRGQWEAARSLGLPYYKALFKVVAPQGIKIMVPSLVNQLIITLKDTSILSAIGVMELTQTGTVIISRNLEGFKMWLIIATMYVIIITVLTWLSNYVQRKMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJR2</accession>
    <name>H4GJR2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC superfamily ATP binding cassette transporter, ABC protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_5952</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000047">
        <property type="protein sequence ID" value="EHS86212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122295.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000047.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5952"/>
    <dbReference type="EnsemblBacteria" id="EHS86212">
        <property type="protein sequence ID" value="EHS86212"/>
        <property type="gene ID" value="PS3_5952"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1068"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="224" mass="24657" checksum="BCA3F03668A6C472" modified="2012-04-18" version="1">MAEIELTNINKFFGEDTARVHVLKDLNFTANPGELVLILGPSGSGKSTFLTIAGGLQTPDSGSVLVDGQDVYQLSSKQRDRLRLNKIGFVLQAYNLVPYLTVLEQFKLADKVKATGNMNPAQLDELLKDLGIYELRYKYPDQLSGGQKQRVAIARALYSQPKIVLADEPTAALDSPRVAEVGALLADLAHKQNRAIVVVTHDVRLEEFADQTYRLSDGVMTKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJS8</accession>
    <name>H4GJS8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_3785</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122311.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3785"/>
    <dbReference type="EnsemblBacteria" id="EHS86115">
        <property type="protein sequence ID" value="EHS86115"/>
        <property type="gene ID" value="PS3_3785"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1100"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="2">
        <location>
            <begin position="33"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="389" mass="42986" checksum="03F49DFC55CF1388" modified="2012-04-18" version="1">MKINQASRINGETDNFLSRQQELIRELKQAGHEVISLGRGSPDLPTFPEIVDEFLQDSKKPLNHGYPPYGGKQTLKVAIQEFYQSEYGVELSLDEITIFSGSLAAITALPMVLLNPGDYALIPDPAFFGYQNGVKMAGGRSYPLPLKADHQYLPSYDQIPPVVLKQANLLFLNYPNNPTGAGATREFFEETVSFAKQNQIPVVHDFAYADISFHQSAPSFLSVPGAKDVGVELYTLSKTFNMAGWRIAFAVGNQEIIHLIEGFIRASFGGVFGAIQDAVEFGLLNSQKGRANLRAIYQDRQVAAVKLLKDHHIEVLDSAGTFFLWIKLPNGVDDVQFANDLLKEKYVAVVAGSTFGEYGKGYIRVSLVDQTKTILEALKRLVSFIEENG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJT9</accession>
    <name>H4GJT9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromosome partition protein Smc</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">smc</name>
        <name evidence="6" type="ORF">PS3_4958</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Required for chromosome condensation and partitioning.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">Contains large globular domains required for ATP hydrolysis at each terminus and a third globular domain forming a flexible hinge near the middle of the molecule. These domains are separated by coiled-coil structures.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SMC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122322.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4958"/>
    <dbReference type="EnsemblBacteria" id="EHS86126">
        <property type="protein sequence ID" value="EHS86126"/>
        <property type="gene ID" value="PS3_4958"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1117"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01894">
        <property type="entry name" value="Smc_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011890">
        <property type="entry name" value="SMC_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="SSF75553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02168">
        <property type="entry name" value="SMC_prok_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="5">
        <location>
            <begin position="519"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="283"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="672"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="756"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="32"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS86126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="1188" mass="133321" checksum="A697269057AE2672" modified="2012-04-18" version="1">MRLLSLEIEGFKSFAQKTKIEFQPGMNGIIGPNGSGKSNIIEAIRWVMGEQFAKTLRGDKMADVIFNGSTDRKPLNRAQVKITFDNSDHYLQSEFTELTVTRRLYRNGDSEYLINDHAVRLKDIVELFMDSGIGRESFSIISQGRVAEIFNGKPSDRRTVIEDVAGVSKYKQNKQVAEKRLAETNDNLNRVNDIITEIMDRLEPLAEQSALAQDYLEQKTRLDLLDRTQTVRSILTKRQQLGTVQDQLAHAKATSDQYETETKSANEHLLVLRETYQRQLKAKDEHQQQVLSLTELIAKLNGEQSLSSVRQEQRAQEIGRLQTAIQSLKEQLAVLQKKLGQDRETITKQSQQLASDQAALKAAQSLSAREQVAQLEEQVETKRSQLVDLMQEQTSVQNQQQYLKRNHQRDSDQQAQADTDLKNLQEQYEEANQKAQAQHQQVAASQAKLDRISAKLQAEQAQAQTIQQRYEQISQQWYQALGEVKSAQNRIQGFRSMAADYTGYYQGVQYVLKNRQRFTGLFGSVSELIQVAPQYTMAIETVLGSQLQQLVVDTQITGKAIINFLVQQRAGRVTILPMDNLRPSWTPNSLQRVQTLPGFIGQASELIQYQDQYKGVVAHLLSTTVVADNLDHATTIARAGQHQLRVVTLDGQLINASGAMTGGANRRQRTGLLQQKEQLAQLETALKKTQANASELEHQVQNIQTAREQNQAVLTKLQEEQGQLQNAHQTLVTESQVSANELTSLKRQLTSLEFTVNQQNSQSQNYQSQLDQLDRDYARLSQEIADHQTAIQQMKDQINDLQQNANSQAERIQELMQKTAVGQERLNQLKSQQNADQVQLADYEQSQADYQHQLDQLESSVQAQADEVENSQAALQKATVELTDQQALVEKLSTETDAMAEQIENETTQLDRLQGLQRVAMNELSTLSGQQAKLEAQIDQGLNRLSERYSMSLTDAQTDLSELDDDELARQIKLLNRGIAELGEVNTASIAEYEEVKTRYDFLAGQQADLLESRAQLDATMSEMDREVATRFIKTFEEVSTAFSATFKQIFAGGSAKLVLTDPDAPLTTGVDIIAQPPGKRNQNLSLLSGGEQALTAITLLFAIIQVRPVPFAILDEPEAALDAVNVDRFANYLSHFGDDGPQFIVITHRKGTMMNANILYGVTMQESGVSRMVAVDIDETLGQHQAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJU8</accession>
    <name>H4GJU8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit biogenesis GTPase RsgA</fullName>
            <ecNumber evidence="7">3.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rsgA</name>
        <name evidence="10" type="ORF">PS3_1960</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Helps release RbfA from mature subunits. May play a role in the assembly of ribosomal proteins into the subunit. Circularly permuted GTPase that catalyzes slow GTP hydrolysis, GTPase activity is stimulated by the 30S ribosomal subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer. Associates with 30S ribosomal subunit, binds 16S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class YlqF/YawG GTPase family. RsgA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122331.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_1960"/>
    <dbReference type="EnsemblBacteria" id="EHS86135">
        <property type="protein sequence ID" value="EHS86135"/>
        <property type="gene ID" value="PS3_1960"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1126"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04466">
        <property type="entry name" value="S1_YloQ_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01854">
        <property type="entry name" value="YjeQ_EngC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01820">
        <property type="entry name" value="GTPase_RsgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004881">
        <property type="entry name" value="Ribosome_biogen_GTPase_RsgA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010914">
        <property type="entry name" value="RsgA_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031944">
        <property type="entry name" value="RsgA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32120">
        <property type="entry name" value="PTHR32120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03193">
        <property type="entry name" value="RsgA_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16745">
        <property type="entry name" value="RsgA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00157">
        <property type="entry name" value="TIGR00157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50936">
        <property type="entry name" value="ENGC_GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CP-type G" evidence="9">
        <location>
            <begin position="58"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="ENGC_GTPASE" evidence="8">
        <location>
            <begin position="67"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="107"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="162"/>
            <end position="170"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS86135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="289" mass="32591" checksum="C4C3230B4E266CF1" modified="2012-04-18" version="1">MQTGIIQQSLSGFYDILSDHQIFRTRARGNFRKRGIKPVVGDRVEFEDGYLLKVLPRDNQLIRPMVANVDVAVVVTAMKEPGFSTNLLDRQLIALESQQIVPVIYFTKTDLLSDQEAQEFQSVIKGYQEIGYATFFSRPPFEQALLDNLMTALDGKIVTMMGQTGAGKSTLLNHLAPDLNLATGEISNALQRGKHTTRKVSLLQVQGALIADTPGFSSYEDFPMTVQELPDYFPEMRRISGDCRFRGCLHLKEPGCAVKKAVEDGIIMCSRYENYQQFHELIMNQKPKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJV8</accession>
    <name>H4GJV8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_17970</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000050">
        <property type="protein sequence ID" value="EHS86032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122341.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000050.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17970"/>
    <dbReference type="EnsemblBacteria" id="EHS86032">
        <property type="protein sequence ID" value="EHS86032"/>
        <property type="gene ID" value="PS3_17970"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1138"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS86032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="366" mass="40384" checksum="FDAFC27C5F6C89F7" modified="2012-04-18" version="1">MKKTLAIAMRVLKQLLRDKRTLALMFIAPILIMWLLNVMFSANTTTNVKLATVDLDSALVTQLDQTKHVTVKEYQSKAQANRTVKNDRADAAIVQTSTNHYSITYANTDSSKTTMTKQVFQQALTKVGVRQLTTTMQTMAKATGTSVPSATTSTKITNHYDYGNSDTGFFTKMVPILMSFFIFFFVFLISGMALLNERTTGTLDRLLATPVRRAEIVFGYMLSYGLVAIVQTIVIVWATIGLLNIEVVGSIFSIVIITAILAFVALAFGILMSTFANSEFQMMQFIPLVIVPQVFFSGIIPLDQMASWVQVIGKILPITYAGDALSQIILHGTSITDLGGDILALLIFLIILTTANILGMKRYRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJW7</accession>
    <name>H4GJW7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA-3-methyladenine glycosylase I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_21108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000050">
        <property type="protein sequence ID" value="EHS86041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21108"/>
    <dbReference type="EnsemblBacteria" id="EHS86041">
        <property type="protein sequence ID" value="EHS86041"/>
        <property type="gene ID" value="PS3_21108"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1152"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005019">
        <property type="entry name" value="Adenine_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03352">
        <property type="entry name" value="Adenine_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="188" mass="21634" checksum="8DB06ECBA2246513" modified="2012-04-18" version="1">MIKRCDWAQSTPLMQNYHDYEWDRPVHNEQKLFEILTLEMFQAGLSWAIVLKKRETIRQAFLNFDFSRMSQLDVDLLMQNSGIIRYRRKIEAVLHNASRMQAIQETGGLNQLVWSLVDNKTIDHQVHRIDQVPTSSPVAIQLSNDLKSAGFKFFGPTTVYSFMQAAGVVNDHLVDCIVHDQIGGVNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJZ4</accession>
    <name>H4GJZ4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_21464</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS86011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122377.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21464"/>
    <dbReference type="EnsemblBacteria" id="EHS86011">
        <property type="protein sequence ID" value="EHS86011"/>
        <property type="gene ID" value="PS3_21464"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1204"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="15"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="329" mass="37237" checksum="63B98A6EAF324535" modified="2012-04-18" version="1">MEYTRLGNTGLQVSKICLGGMSFGDANLGGMHQGWILDEEGTRRIIKKALSIGINFFDTANIYGYGTSEEYVGRALKDFANRDEVIIATKLFFGDSLEPAPNTTGLSRKAIFNQVEASLRRLGTDYIDILYIHRWDYETPIEETMAALNDLVRAGKVRYLGASAMYTWQFQKAQYVADKHGWTKFSVMQNHYNMLYREDEREMMPFCKDSGVGLAPYSPFAAGRVIRDWDADTARSQTDEIAKKKYDATEEQDLAIVKRVAELADRYQVSRSQIALNWLWKKGIDSPIVGVTKEAYLDDFAGAFDVTLTDEDVAYLDEKYLPHKIVGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJZ8</accession>
    <name>H4GJZ8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PPK2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_15820</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000052">
        <property type="protein sequence ID" value="EHS85880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122381.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000052.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15820"/>
    <dbReference type="EnsemblBacteria" id="EHS85880">
        <property type="protein sequence ID" value="EHS85880"/>
        <property type="gene ID" value="PS3_15820"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1214"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008976">
        <property type="term" value="F:polyphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006797">
        <property type="term" value="P:polyphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016898">
        <property type="entry name" value="Polyphosphate_phosphotransfera"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022300">
        <property type="entry name" value="PPK2-rel_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022488">
        <property type="entry name" value="PPK2-related"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03976">
        <property type="entry name" value="PPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028756">
        <property type="entry name" value="PPK2_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03709">
        <property type="entry name" value="PPK2_rel_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PPK2" evidence="3">
        <location>
            <begin position="28"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="291" mass="33609" checksum="D2E80448BFFBD3B1" modified="2012-04-18" version="1">MEEQYKFVTGDLKLADVPTVVDNPLSLKEAKAVVKKQRKAMSKLQPKLYAQHKYGVVIVLQALDAAGKDSLIAHTFSGLNPAWFDVVNFKQPSSIDLDHDFLWRINQQLPARGMLGIFNRSYYEDVLISRVHPEILLNENLPGIDGLADLNEEFYQNRYHDIQNYEDYLNHAGYVVIKIFLHISKDEQKQRFLDRVNTPDKQWKFSASDVHEHKFFDQYIDAYQRAINATATKDNPWYVIPADDKWNSRAIAATIIRERLEQLPLAFPEIAKEDNAKLKAAAKELKKEAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK01</accession>
    <name>H4GK01_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Eps transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_3343</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LytR/CpsA/Psr (LCP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000053">
        <property type="protein sequence ID" value="EHS85873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122384.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000053.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3343"/>
    <dbReference type="EnsemblBacteria" id="EHS85873">
        <property type="protein sequence ID" value="EHS85873"/>
        <property type="gene ID" value="PS3_3343"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1223"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004474">
        <property type="entry name" value="LytR_CpsA_psr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03816">
        <property type="entry name" value="LytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00350">
        <property type="entry name" value="lytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="LytR_cpsA_psr" evidence="3">
        <location>
            <begin position="94"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="334" mass="37728" checksum="C86B4C6B0E98260D" modified="2012-04-18" version="1">MDSEEIKHHHHHHHRRHKHHWLRWLWVLIGVAFVIAVIFAAMAWRNLRDATNGMYSSSGVSGRSISSVLKQKKPISILLLGTDTGALGRTEKGRTDTIMMMTINTQTNKTTIVSLPRDMKVDLPDYPDYSPAKINSAYTYGGVKETIKVIKEQFGIPVDAYLLVNMGSMEKAIDQVDGIDVKSPLTFTYEGYSFKKGKTYHMDGKEALAFSRMRYDDPDGDYGRQSRQRLIIAALIKESASYKSVLNRKFLQTLSGGMQTNLTFSNMTQLALNYRDATKVVQDYASGTSDDEDGVSYQQVSYNERQRISNLIRQSLGLQTKTLPDDDDSDSSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK17</accession>
    <name>H4GK17_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_19309</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000054">
        <property type="protein sequence ID" value="EHS85869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122400.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000054.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19309"/>
    <dbReference type="EnsemblBacteria" id="EHS85869">
        <property type="protein sequence ID" value="EHS85869"/>
        <property type="gene ID" value="PS3_19309"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1243"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045276">
        <property type="entry name" value="YbiO_bact"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30460">
        <property type="entry name" value="PTHR30460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="SSF82861"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="287" mass="31613" checksum="8EEF6D6FAFEDD542" modified="2012-04-18" version="1">MNSLISKQAQLAAKSFTNLNWDRLAQNIVDKAFSLIMTILIFFLILGIGKRIINSIFHNTKRITLLGGSHRAETFHSIILNIYRYTCYFFFLYAILSLIGVPIGTLIAGAGIFSIALGLGAQGFVSDVVNGFFILLEQQIDVGDIVQIGTIKGTVKAVGLRTTQVLGSDGTLTFIQNRNITMVQNFSRHDLTANVDIAITTKTPLNELKLVVENLTDEIMNDFDNLTSQPQIIGPTTNQNGQLVYRIMITAKSGTQTVTASKILARYLQAAQQAQIELQTTSHYPIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK59</accession>
    <name>H4GK59_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamyl-tRNA(Gln) amidotransferase subunit A</fullName>
            <shortName evidence="8">Glu-ADT subunit A</shortName>
            <ecNumber evidence="8">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gatA</name>
        <name evidence="10" type="ORF">PS3_8623</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000059">
        <property type="protein sequence ID" value="EHS85693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122442.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000059.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8623"/>
    <dbReference type="EnsemblBacteria" id="EHS85693">
        <property type="protein sequence ID" value="EHS85693"/>
        <property type="gene ID" value="PS3_8623"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1304"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Amidase" evidence="9">
        <location>
            <begin position="24"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="8">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="490" mass="52574" checksum="26B80162046A841A" modified="2012-04-18" version="1">MDYRQTDISQLHADLVAGKVTATELVKASLARMDETEADVQAFITRNDEAALAKAKALDEAGIPADQLLAGIPLAVKDNILTKGLRTTAASKILENFMPVYDATVIEKLNAAGFINVGKTNLDEFAMGSSTETSYFGTTHNPWDLSRVPGGSSGGSAAAVAAGDVLGAFGTDTGGSIRMPASFNGIVGMKPTYGRVSRWGVIAFGSSFDQVGWLTQNVKDNAILMTAISGQDDHDMTSSRRPVPDFAAGLDEANVKGLRIGVPKEYMSDAIDADVREVIQAGLDHLVSLGATVDEVSLPHTKYGVPAYYILASSEASSNLQRFDGIRYGFRADNVKNLEDVYVKTRSEGFGDEVKRRIMLGTFSLSAGFYDAYFNKAAKIRRLIAEDFEKVFADHDVIIGATGTSTAFKIGENIDDPTKMYYNDVLTVTANLAGVPAMSVPAGFSKVNGMPVGMQLIGKKYDEATLYKTAYVFEQTTDHHKQTAKLGGDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK99</accession>
    <name>H4GK99_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">PTS system sucrose-specific IIABC component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PS3_11004</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122482.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11004"/>
    <dbReference type="EnsemblBacteria" id="EHS85551">
        <property type="protein sequence ID" value="EHS85551"/>
        <property type="gene ID" value="PS3_11004"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1361"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00212">
        <property type="entry name" value="PTS_IIB_glc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036878">
        <property type="entry name" value="Glu_permease_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018113">
        <property type="entry name" value="PTrfase_EIIB_Cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001127">
        <property type="entry name" value="PTS_EIIA_1_perm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003352">
        <property type="entry name" value="PTS_EIIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013013">
        <property type="entry name" value="PTS_EIIC_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001996">
        <property type="entry name" value="PTS_IIB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010973">
        <property type="entry name" value="PTS_IIBC_sucr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00358">
        <property type="entry name" value="PTS_EIIA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00367">
        <property type="entry name" value="PTS_EIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02378">
        <property type="entry name" value="PTS_EIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55604">
        <property type="entry name" value="SSF55604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00826">
        <property type="entry name" value="EIIB_glc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00830">
        <property type="entry name" value="PTBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01996">
        <property type="entry name" value="PTS-II-BC-sucr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51093">
        <property type="entry name" value="PTS_EIIA_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51098">
        <property type="entry name" value="PTS_EIIB_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01035">
        <property type="entry name" value="PTS_EIIB_TYPE_1_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51103">
        <property type="entry name" value="PTS_EIIC_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIB type-1" evidence="14">
        <location>
            <begin position="3"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIC type-1" evidence="15">
        <location>
            <begin position="108"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIA type-1" evidence="13">
        <location>
            <begin position="517"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate; for EIIB activity" evidence="11">
        <location>
            <position position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EHS85551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="647" mass="68395" checksum="A39A33A78CFFD005" modified="2012-04-18" version="1">MDHKQVAADVVKAVGKDNMVAAAHCATRLRLVLKDESKVDQKALDANDGVKGTFSTNGQFQIIIGPGDVNNVYDYLIKETGLKEVSTDDLKQIAAKNQKFNPVMAFIKLLSDIFVPIIPALVAGGLLMALNNVFTSKFGSESIIQMYPALKGISSMISVMSAAPYTFLPILVGVSAAKRFGANQYLGAFVGMMMTITATNPDAIKGIAYWNLFGLHVSYAAYTSQVIPALAAVWLLSVFEKWFHKHLPSAVDFTFTPLLSVLLTGFLTFIIVGPVMRELSNWLTDGIVWVYNTLGFVGTGIFGAVYSPIVLTGLHQSFPAIETQLVSAFANGKGSGDFIFVVASMANVAQGAATTAVYFLTKDSKMKGLSSSSAVSALLGITEPAIFGVNLKLRFPFFCALVGSCVASTLAGLFHVIAASMGSAGFIGFISIYPKSIPFYLICELISFAIAFIITYLYGKSHMQLVDGDVVAGADDNAATVAAAEETTTTAPSAGADVVTIASPVSGQVEDLSQVNDEVFSQKMMGDGAAVVPSDGTIYAPADGTLSVAYATKHAYGITADNGAEILIHAGIDTVNLEGQHMESFVEQGQTVKKGDKLGTIDLEAVKAAGYDTTVMVILTNTANYQTVSRDADGEVTHGDDLFTAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKA1</accession>
    <name>H4GKA1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Sucrose-6-phosphate hydrolase</fullName>
            <ecNumber evidence="3 8">3.2.1.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 9">Invertase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PS3_14598</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Enables the bacterium to metabolize sucrose as a sole carbon source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.</text>
            <dbReference type="EC" id="3.2.1.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Glycan biosynthesis; sucrose metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyl hydrolase 32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.26" evidence="3 8"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122484.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14598"/>
    <dbReference type="EnsemblBacteria" id="EHS85553">
        <property type="protein sequence ID" value="EHS85553"/>
        <property type="gene ID" value="PS3_14598"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1363"/>
    <dbReference type="UniPathway" id="UPA00238"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004564">
        <property type="term" value="F:beta-fructofuranosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005985">
        <property type="term" value="P:sucrose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001362">
        <property type="entry name" value="Glyco_hydro_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018053">
        <property type="entry name" value="Glyco_hydro_32_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013189">
        <property type="entry name" value="Glyco_hydro_32_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013148">
        <property type="entry name" value="Glyco_hydro_32_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006232">
        <property type="entry name" value="Suc6P_hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08244">
        <property type="entry name" value="Glyco_hydro_32C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00251">
        <property type="entry name" value="Glyco_hydro_32N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00640">
        <property type="entry name" value="Glyco_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="SSF75005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01322">
        <property type="entry name" value="scrB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00609">
        <property type="entry name" value="GLYCOSYL_HYDROL_F32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 8 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_32N" evidence="10">
        <location>
            <begin position="37"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_32C" evidence="11">
        <location>
            <begin position="347"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS85553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="491" mass="56170" checksum="F4D9AAEDF2079E9F" modified="2012-04-18" version="1">MEWTREQRYRPYDQHSALELLKLQRQADNSPEQLKYHIRPTSGLLNDPNGFSYFNKEWHVFYQSFPFGAAHGLKSWMHMTSPDLVHWRSHGLALAPDTEYDSHGAYSGSARVVNGRLCLMYTGNHRDDDWIRTPYQLGAWMDHNYQVTKFDQPLFVNPSTITEHFRDPQLLEHDDRFYAIIGSQDAEKKRGHIDLWTSDQLEYGWHELGHVDLGQDDLGYMVECPNLITVDGRQVMIFCPQGLDHQVAPYNNIYPDMYLTAESFDYDHPAMINPDAHMQNLDDGFDVYATQAMNTADGKAYAISWVGLPDLEYPTDNQNWANCMSQVKELHYHDGQLYQSPVPAIRDLRQTDQAINIDGQVETINPEAGQQFELNLTLPADQTGTLHVAAKDDLSKSVAINFDTQAGKLTIDRGHTDLPTNLDYGTSRTVDLPAHQTLSLDIFVDHSLLEVFINDGAKVMTLRYFNNQANQMLAFDHPVQATGTWWPLSDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKA4</accession>
    <name>H4GKA4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_9407</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9407"/>
    <dbReference type="EnsemblBacteria" id="EHS85556">
        <property type="protein sequence ID" value="EHS85556"/>
        <property type="gene ID" value="PS3_9407"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1371"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011951">
        <property type="entry name" value="HAD-SF_hydro_IA_YjjG/PynA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02254">
        <property type="entry name" value="YjjG/YfnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS85556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="225" mass="26012" checksum="A288C87456372856" modified="2012-04-18" version="1">MDNTLFDFDAGEHQALTELFRRHQWSLPDDRRQRYLQYNKGLWRMTERGEITKDQLFQNRFKDFIWAEFAIKIGPEVDQEYLNLLAHQAQLLPGAMETLRLAKELGFEMNVITNGAKLTQAQRLELSGIKPYFNTIIISSEVGIEKPDPQIFATLFEQQHLQKQGSLMIGDGVASDLTGAQQFGIDSLWYNIYQETLPKELSVKYQVMNHDQLQATLRKIAKSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKB1</accession>
    <name>H4GKB1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">dUTP diphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_3962</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dUTP + H2O = diphosphate + dUMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.6.1.23"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122494.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3962"/>
    <dbReference type="EnsemblBacteria" id="EHS85563">
        <property type="protein sequence ID" value="EHS85563"/>
        <property type="gene ID" value="PS3_3962"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1379"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046081">
        <property type="term" value="P:dUTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008181">
        <property type="entry name" value="dUTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241">
        <property type="entry name" value="PTHR11241"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase" evidence="4">
        <location>
            <begin position="73"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="178" mass="20299" checksum="4909C6C3C600BE35" modified="2012-04-18" version="1">MKRGFQVVSFKADQKINLPQRQTQQAAGYDLEAAEDFVLPSIWKGNFLKTLWKIHQQKELTNDDITQADQSLKPYLVPTGIKAYMQADEVLMLFNRSSGPLKRRLVLPNSVGIIDADYYNNPQNEGEIFVQLLNYGLTDYHIKKGERIAQGIFMPYLTADEELQPSQKRQGGFGSTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKC5</accession>
    <name>H4GKC5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Lactose transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_18158</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the sodium:galactoside symporter (TC 2.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122508.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18158"/>
    <dbReference type="EnsemblBacteria" id="EHS85577">
        <property type="protein sequence ID" value="EHS85577"/>
        <property type="gene ID" value="PS3_18158"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1396"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039672">
        <property type="entry name" value="MFS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001927">
        <property type="entry name" value="Na/Gal_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001127">
        <property type="entry name" value="PTS_EIIA_1_perm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11328">
        <property type="entry name" value="PTHR11328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00358">
        <property type="entry name" value="PTS_EIIA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00792">
        <property type="entry name" value="gph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00830">
        <property type="entry name" value="PTBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51093">
        <property type="entry name" value="PTS_EIIA_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00371">
        <property type="entry name" value="PTS_EIIA_TYPE_1_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="256"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="403"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="434"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIA type-1" evidence="9">
        <location>
            <begin position="505"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="638" mass="70320" checksum="43411E963B812B8F" modified="2012-04-18" version="1">MTNSNAPSKHLFSKFSFAMGNFGHAAFYGALSNYFIVYVTSVMFVGVSSRIADKLIGLITGLIVLIRILEIFINPLLGNIVDNTHSRWGKFKPWILSGTIISSVLLIALFAGLFGLIKVNWILYAIVFVIVFVVMDIFYSFSDVSYWGMVPALSEDSAERGVYTSLGTFTGAIGWNGLTIIVVPVVTYFTYLTTSKHEQGAPGWFAFAVIISLLAIVCATLVTIGTHEKDNLLRRSAQQKTTIKDVISALAHNDQLLWTCLAYFMYSLANTITTGVLYYFFKFVLGKPGTYWIVGMAAMIISFCTSPLYPVLNRFIPRKWLYTLGMVCMMIAYLIFIFFHDNLTLLVIGLVLYYFTFAQLVSILTMTDSIEYGQLKNGERNEAVILSIRPLIDNLTGAFSNGLVGYIAIAAGMTGAATAADMTAKNIATFNSPAFYIPFALSILSLVIFLTKVKLSEKAHAEIVDELTLKLAAGQSNLTDDQPTNSKPASTKVFAPVDGNLIPMDQVKVNGQPFPGTGFAIRPSDGHIYAPFDGRIVFTFSSKHLIGLVSDQGLEAIIHIGIDTVNLNGQGFVSHYSDGQTIQAGDLLMDFDRELINQNHYDDTVIMFFTKPQQLLPLASINERPINHGDLIEQVQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKC7</accession>
    <name>H4GKC7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription accessory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_7292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122510.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7292"/>
    <dbReference type="EnsemblBacteria" id="EHS85579">
        <property type="protein sequence ID" value="EHS85579"/>
        <property type="gene ID" value="PS3_7292"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1398"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05685">
        <property type="entry name" value="S1_Tex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3500.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041692">
        <property type="entry name" value="HHH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044146">
        <property type="entry name" value="S1_Tex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023323">
        <property type="entry name" value="Tex-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023319">
        <property type="entry name" value="Tex-like_HTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018974">
        <property type="entry name" value="Tex-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032639">
        <property type="entry name" value="Tex_YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006641">
        <property type="entry name" value="YqgF/RNaseH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17674">
        <property type="entry name" value="HHH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09371">
        <property type="entry name" value="Tex_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16921">
        <property type="entry name" value="Tex_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00732">
        <property type="entry name" value="YqgFc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="648"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="724" mass="80938" checksum="5D1305CCF93E79A6" modified="2012-04-18" version="1">MEATIVKNVEATLTEFKPWQVKAALELMDEGNTIPFISRYRKEATGTLDEVQLQTIQTTYHKAQELADRKQTVLKAIAEQDKLTDALKQQIESAPDLTTVEDLYLPYKQKRRTKAQIAKEQGLMPLANWLLHYEDTDLTTKAAEFVTDEVADAPTALSGAHEILAEAISEMSTVRAWLRDYTTKNGRIVTKVTKQGATIDEKGVYQQYYDFDAVIKDMTSYRTLAINRGEKEKVLRVKIEVASEPIDNYLAFRLIRGHVENDATRFVMAAAQDAYKRFLGPAIERELRHQMTEEADEQAINVFGENLYHLLMQAPIKGKMVLGFDPAYRTGCKLAVLDPSGKLIQTAVIYPHPPAPEAKRAQAQQAFLDLINQYQIEMVAIGNGTASRESEQFVATALKQLDRDVYYVIVNESGASVYSASQDARDEFPDLTVEKRSAISIGRRLQDPLAELVKIDPQAIGVGQYQHDLPETELKTELTAVVERAVNRVGVNLNTASYQLLTYISGLSATIAKNIVKYRDENGQYVDRTQLKKVSRLGPKAYQQAVGFLRIVDGDNPLDNTDIHPESYPVATQILQDAQVTVSELGSSSANQALQALDPQTYVTDTVGLATVTDVINDLQKPGRDLRDSMPAPLLHQDVMTIDDLKVGMQLEGTVRNVVDFGAFVDIGVKHDGLVHVSRMSKKFIKDPRQVVSVGDIVTVWVYEVDLDRQRIQLTMLPPQNEHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKC8</accession>
    <name>H4GKC8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">NH(3)-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="4 6">6.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">nadE</name>
        <name evidence="9" type="ORF">PS3_1435</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 6">
            <text>ATP + deamido-NAD(+) + NH4(+) = AMP + diphosphate + H(+) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21188"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 7">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.5" evidence="4 6"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122511.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_1435"/>
    <dbReference type="EnsemblBacteria" id="EHS85580">
        <property type="protein sequence ID" value="EHS85580"/>
        <property type="gene ID" value="PS3_1435"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1399"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00333"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00193">
        <property type="entry name" value="NadE_ammonia_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022926">
        <property type="entry name" value="NH(3)-dep_NAD(+)_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="PTHR23090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_synthase" evidence="8">
        <location>
            <begin position="24"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="46"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="261"/>
            <end position="262"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS85580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="276" mass="30524" checksum="2CC4D05162006C86" modified="2012-04-18" version="1">MTTLQATIIKDLQVKAQIDPATEIQQRVQFIQEYLKTTGMKTLVLGISGGQDSSLAGRLSQLAVESLRQTTHDENYQFIAVRLPYGEQADESDAMFAINEFIKPDRMIRVNIQPATDAMVASLQAGGETISDFNKGNIKARERMIVQYAIAGQHQGAVVGTDHAAEAVTGFYTKFGDGGADLTPLTGLNKRQGKQLLTYLNAPAKLYEKTPTADLEEDRPALPDEVALGVTYQNIDDYLEGKNVDQAVADKIEAWYQKTGHKRHLPVAPQDEWWKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKD5</accession>
    <name>H4GKD5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_19993</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000063">
        <property type="protein sequence ID" value="EHS85499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122518.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000063.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19993"/>
    <dbReference type="EnsemblBacteria" id="EHS85499">
        <property type="protein sequence ID" value="EHS85499"/>
        <property type="gene ID" value="PS3_19993"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1426"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028259">
        <property type="entry name" value="AP2-like_int_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14657">
        <property type="entry name" value="Arm-DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14659">
        <property type="entry name" value="Phage_int_SAM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="3">
        <location>
            <begin position="163"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="373" mass="42973" checksum="04A1542729463ABD" modified="2012-04-18" version="1">MAQYTKRGAKWQARIQWRDSHGKRHTKSKLGFATKSQAKQWAVEVESSLMKGIKLDRSITLYDYWSHWVATYKAPTLSKISLSRYNSFGNLIRQSFGDTKISDISRSQYQEFINDFGQNHAPSTVQKLNGFIRACINSAILDDYLVKDFTQGVALTSDRSRIVKVEYLDRSEIERIINTLTDDLNSNYTSRYMILTAIYTGMRLSEIQALTWSDIDFNKQTIKINKSWDFRTRSFKPTKNESSNRTIRINTELLDILNQLRRPAKGTMLFINQFGTIPTSNAVNKCLRSIMNDLGIKRANFHFHSLRHSHVAILLAKGIDIYAISKRLGHSDVSTTINTYAYLLDEYKQQTDNQIIDALNFGTHTAHKTQPYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKE6</accession>
    <name>H4GKE6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_12045</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000064">
        <property type="protein sequence ID" value="EHS85438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003649842.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000064.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12045"/>
    <dbReference type="EnsemblBacteria" id="EHS85438">
        <property type="protein sequence ID" value="EHS85438"/>
        <property type="gene ID" value="PS3_12045"/>
    </dbReference>
    <dbReference type="GeneID" id="66535900"/>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1448"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS85438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="92" mass="11148" checksum="967A89F369B794AC" modified="2012-04-18" version="1">MSNQYEKLVEQQARLKQKIEREDFKLRQSKYYENRQARKARSRRLIQKGALLEKYFQADNLSVEQTEELLKIFANYVNAHKPNKFKNDQPNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKH0</accession>
    <name>H4GKH0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glycerol-3-phosphate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_11833</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000067">
        <property type="protein sequence ID" value="EHS85384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122552.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000067.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS85384">
        <property type="protein sequence ID" value="EHS85384"/>
        <property type="gene ID" value="PS3_11833"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1496"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000849">
        <property type="entry name" value="Sugar_P_transporter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002808">
        <property type="entry name" value="Hexose_phosphate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="27"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS85384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="432" mass="46934" checksum="BE6A6236BB7A9DB2" modified="2012-04-18" version="1">MFNWLLPSAPATQLIPNDQQPRAYLKYRSRILISTILAYAAYYVVKSNFTLASPHLTKYAGFTTAQVGLVLSALAISYGIGKFFVGLLVDRMNPRFSVGIGLILSAIFNWCFGYTHSLGVMIILMIFVGITQGFGAPSCQKSLATWFDAENLGTATSTWNISHNIGSGTASIIVATIVSIYGAANIQNIFMIPSIISIVMAIIVMTMGVDTPESVGLPRVNKDEQKNIKHKTTWGAFRDNILKNPIVWAFCIMNAFSYILRYGIENWIPTYLSTGKGFSSVITNTGYTVFEYAAIPGTILIGLFSDKVLKGRRVPITLACSTLVLIATPLYAGTHSVTITYLLLGLMGICIYAQQVLMGIVIMDVLPIESVAGGIGLSGFFGYAFGEVIASYGIGSIVGSNNWNGAFGIVAISAIICIVFFIFIEFKYHHKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKH9</accession>
    <name>H4GKH9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lysozyme</fullName>
            <ecNumber evidence="4">3.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_21053</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Hydrolysis of (1-&gt;4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.17" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21053"/>
    <dbReference type="EnsemblBacteria" id="EHS85259">
        <property type="protein sequence ID" value="EHS85259"/>
        <property type="gene ID" value="PS3_21053"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1520"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002053">
        <property type="entry name" value="Glyco_hydro_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008270">
        <property type="entry name" value="Glyco_hydro_25_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018077">
        <property type="entry name" value="Glyco_hydro_fam25_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01183">
        <property type="entry name" value="Glyco_hydro_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00641">
        <property type="entry name" value="Glyco_25"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00953">
        <property type="entry name" value="GLYCOSYL_HYDROL_F25_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51904">
        <property type="entry name" value="GLYCOSYL_HYDROL_F25_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS85259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="477" mass="52042" checksum="C0DB788AA5F78B7C" modified="2012-04-18" version="1">MNQTQANADTTDDSTQTESIVSNNTTSTNSVTLSSSTDTNTTSTTQTSTTSTDNSNTANTTESTTASSSTTTTTHQHLTLAVATSSTFSTSSASVKNGEVTEDDVTYYYVNNEKQSDYFYTDNDGKVYYFGDDGKMYQDQFYSNWGNMYYFGEDGARYTEQFYSNWGNMYYFGEDGVRYTNQFYSNWGNMYYFGEDGVRYTNQFYTNWGNTYYFDEDGVRYTNKLYSNWGNTYYFDSYGALLKNQTVEIDGVTYKADSNGVLTKVNTGKVGISDGAYGVDVSSYQGTNMSGYASNGATFTIVKLTEGTTYVNSKAEAQIDSAQANNQVVLGYHFATFSSNASAAQTEANFAIAEAQALGLPSGSYLALDWETGDGNVVSGSTSANTNAILTFMNAVAAAGYKPLLYSGAYVLRNNVNTSSVISAYPNSLWVASYPTSGAIYSADVAYFPSMDGVAIWQFTDNWKGLGVDGNIAVQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKJ1</accession>
    <name>H4GKJ1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chaperonin GroEL</fullName>
            <ecNumber evidence="6">5.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">60 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Chaperonin-60</fullName>
            <shortName evidence="6">Cpn60</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">groEL</name>
        <name evidence="6" type="synonym">groL</name>
        <name evidence="10" type="ORF">PS3_11029</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 8">Forms a cylinder of 14 subunits composed of two heptameric rings stacked back-to-back. Interacts with the co-chaperonin GroES.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.7" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122573.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11029"/>
    <dbReference type="EnsemblBacteria" id="EHS85271">
        <property type="protein sequence ID" value="EHS85271"/>
        <property type="gene ID" value="PS3_11029"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1535"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="SSF48592"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="SSF52029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="521"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="29"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="86"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="476"/>
            <end position="478"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="492"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="540" mass="56630" checksum="B004FF84E66C6F15" modified="2012-04-18" version="1">MAKELKFAEDARAAMLRGVDKLADTVKTTIGPKGRNVVLEQSYGSPTITNDGVTIAKGIELEDHFENMGAKLVAEVASKTNDIAGDGTTTATVLTQAIVNEGMKNVTAGANPVGIRRGIDLATKTAVEALKKMSHEVKTKDDIAQIASISAANPEVGTLIADAMEKVGNDGVITIEDSRGVETSVDVVEGMSFDRGYMSQYMVTDNDKMEADLDNPYVLITDKKISNIQDILELLQAVVQEGRSLLIIADDITGEALPTLVLNKMRGTFNVVSVKAPGFGDRRKEQLADIAVLTGGTVISDDLGMNLSDVTVAQLGQANKVTVTKDATTIVDGAGDKTAIAERVEQIKKAIAESTSDFDKEKLQERLAKLAGGVAVVKVGAATETELKERKYRIEDALNATRAAVQEGFVPGGGTALVNVIADLEKIEATGDEATGVNIVKAALEAPVRQIAENAGLEGSVIVNQLKNEKPGVGYNAAEDKFEDMVAAGIVDPTKVTRSALQNAASVSSLLLTTEAVVADKPEDNPAPAAPAGAGMPGMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKJ2</accession>
    <name>H4GKJ2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Co-chaperonin GroES</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">10 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Chaperonin-10</fullName>
            <shortName evidence="3">Cpn10</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">groES</name>
        <name evidence="3" type="synonym">groS</name>
        <name evidence="5" type="ORF">PS3_12666</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Together with the chaperonin GroEL, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding. GroES binds to the apical surface of the GroEL ring, thereby capping the opening of the GroEL channel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heptamer of 7 subunits arranged in a ring. Interacts with the chaperonin GroEL.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the GroES chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122574.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12666"/>
    <dbReference type="EnsemblBacteria" id="EHS85272">
        <property type="protein sequence ID" value="EHS85272"/>
        <property type="gene ID" value="PS3_12666"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1536"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00580">
        <property type="entry name" value="CH10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018369">
        <property type="entry name" value="Chaprnonin_Cpn10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="PTHR10772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00681">
        <property type="entry name" value="CHAPERONINS_CPN10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="93" mass="9912" checksum="E4638D94B55164BD" modified="2012-04-18" version="1">MLKPLGDRVVLKAEDEEQTVGGIVLASNAKNKPTTGTVVAVGPGKALDNGQVRELSVKEGDKVLFDKYAGSEVEYNGDKYLVVHENDLVAVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKL7</accession>
    <name>H4GKL7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA-directed DNA polymerase III epsilon subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_3003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122599.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3003"/>
    <dbReference type="EnsemblBacteria" id="EHS85186">
        <property type="protein sequence ID" value="EHS85186"/>
        <property type="gene ID" value="PS3_3003"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1574"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Exonuclease_RNaseT/DNA_pol3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Exonuclease" evidence="2">
        <location>
            <begin position="2"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="177" mass="20228" checksum="6EE8C514385B645C" modified="2012-04-18" version="1">MDFVAYDFETANNYRFSACSLALIVVRNNQITDELYSLINPQTDFAWRNVAIHGIHEADVADSPTFTELWPHLKTFFTPDQLVIAHNAQFDNSVLKHVLQHYDLPVPSFKSLDTVRTSRKLSPELINHKLDTVSQAYQIDLHHHHNALDDAHACAEILIHQVNNFGVEAIRPFIKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKM0</accession>
    <name>H4GKM0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphate acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_3351</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + phosphate = acetyl phosphate + CoA</text>
            <dbReference type="Rhea" id="RHEA:19521"/>
            <dbReference type="ChEBI" id="CHEBI:22191"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphate acetyltransferase and butyryltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.8" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122602.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3351"/>
    <dbReference type="EnsemblBacteria" id="EHS85189">
        <property type="protein sequence ID" value="EHS85189"/>
        <property type="gene ID" value="PS3_3351"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1577"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012147">
        <property type="entry name" value="P_Ac_Bu_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004614">
        <property type="entry name" value="P_AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042113">
        <property type="entry name" value="P_AcTrfase_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042112">
        <property type="entry name" value="P_AcTrfase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002505">
        <property type="entry name" value="PTA_PTB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01515">
        <property type="entry name" value="PTA_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000428">
        <property type="entry name" value="P_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00651">
        <property type="entry name" value="pta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PTA_PTB" evidence="6">
        <location>
            <begin position="4"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="325" mass="34981" checksum="CB7A865E7E2795D8" modified="2012-04-18" version="1">MDIFQNLADQLKGKNKTIVFPEGEDERVMGAAVRLQADGLVTPLLLGNQDKMAQVAQDHGWDLSVLETLDPTQYPADDLKAMHDALLERRNGKNTSEQVDQMLTDVSYFATMLVYMGKVDGMVSGAVHPTGDTVRPALQIIKTKPGNKRISGAFIMQKGDERYIFADCAINIELDAETMAEVAAQSAETAHMFGLEPRVAMLSFSTKGSAAGEMVTKVAEATKLAQAKIDYPVDGELQFDAAFVPSVGERKAPNSKVAGHANVFVFPSLEAGNIGYKIAQRFGGFEAVGPILQGLNAPVADLSRGTNEDEVYKVALITAAQALED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKN0</accession>
    <name>H4GKN0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADH dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_7757</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122612.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7757"/>
    <dbReference type="EnsemblBacteria" id="EHS84950">
        <property type="protein sequence ID" value="EHS84950"/>
        <property type="gene ID" value="PS3_7757"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1590"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006116">
        <property type="term" value="P:NADH oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="PTHR43706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="3">
        <location>
            <begin position="2"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS84950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="406" mass="44004" checksum="C6493A687AA65CCC" modified="2012-04-18" version="1">MKHVVILGAGYAGLKVVHELQKRVKGDVKLTLVDRNNYHYEATDLHEVAAGTKKPSDISYPIADVVKPSVTTFIQDEVTALDLANQTVKLADHKDLTYDYLVCALGFVSESFGIPGVDEHALPMATLKEAEAIHQHLLAMMDKYQEVQDPSYLNIVIGGAGFTGIELVGALNDARKTLADRAGVQPSDIKINVIEASTHLLPMFSEKLATYGINLVKSFGVNLVTGARITKVDDGQVEFQQGDPEDAVTSTMEAKTIIWTTGVSGSPIIDEAGFSARRGRVIPSEHLTDNDHDNVYLIGDVAAVMPPDGNRPYPTTAQIALGMGKYVANDLASRLTNGQHLTTPFTYKSLGTVASVGNTHAFGEAMGHEVRGYMASATKKMIANESLFRVGGFREVMKKGRFDLYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKN4</accession>
    <name>H4GKN4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome d ubiquinol oxidase, subunit II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_13030</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome ubiquinol oxidase subunit 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122616.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13030"/>
    <dbReference type="EnsemblBacteria" id="EHS84954">
        <property type="protein sequence ID" value="EHS84954"/>
        <property type="gene ID" value="PS3_13030"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1594"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003317">
        <property type="entry name" value="Cyt-d_oxidase_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43141">
        <property type="entry name" value="PTHR43141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02322">
        <property type="entry name" value="Cyt_bd_oxida_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000267">
        <property type="entry name" value="Cyt_oxidse_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00203">
        <property type="entry name" value="cydB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="339" mass="37789" checksum="0A0E5938D9CBA16C" modified="2012-04-18" version="1">MTILQLIWFLLLAVLFSGFFFLDGFDYGVGMAVHTLADNEGERDQIIHSIGPVWDGNEVWLITAGGAMFASFPFWYASLFSGYYLVFLLILVGLILRGVSFEFRAKSAAKGKVFWGWMLTLGSTMVPFLFGMLFISMIHGMPIDANGNFHAGFFDYVNIFSVVGGVAVTLLSYLHGLNYIALKTVGAIRDRAKNYAKFLYWVLYLGLVVFALLLIFQTDFLSVHPIGTLVCLVLVVLFALLAHIALFKDNEMLAFIMSGLTMVGVVALIFTGLYPRVLISSISPKYDLLIQSASSSEYTLTVMTIAAVCILPFVLAYIIWTYWIFRKRIAMPAAQPVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKN6</accession>
    <name>H4GKN6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Elongation factor 4</fullName>
            <shortName evidence="7">EF-4</shortName>
            <ecNumber evidence="7">3.6.5.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Ribosomal back-translocase LepA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">lepA</name>
        <name evidence="9" type="ORF">PS3_8055</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.n1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.n1" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122618.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8055"/>
    <dbReference type="EnsemblBacteria" id="EHS84956">
        <property type="protein sequence ID" value="EHS84956"/>
        <property type="gene ID" value="PS3_8055"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1596"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03709">
        <property type="entry name" value="lepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00071">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006297">
        <property type="entry name" value="EF-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038363">
        <property type="entry name" value="LepA_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013842">
        <property type="entry name" value="LepA_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035654">
        <property type="entry name" value="LepA_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512">
        <property type="entry name" value="PTHR43512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06421">
        <property type="entry name" value="LepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01393">
        <property type="entry name" value="lepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="11"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="23"/>
            <end position="28"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="140"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS84956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="611" mass="68364" checksum="2FB6FEA020383B6D" modified="2012-04-18" version="1">MDLAAMKERQQRIRNFSIVAHIDHGKSTLADRILEMADSISKREMKNQILDDMELERERGITIKLNAVALTYHAQDGEDYIFHLIDTPGHVDFSYEVSRSLAACEGAILVVDATQGVEAQTLANVYLALDNDLEILPVINKIDLPSADAEGTKAQIEEEIGLDTSEAVDISAKSGLGVDQVLEKIVQDIPAPTGDLEAPLKTLIFDSKYDDYRGVVLSVRVFEGTVKKGDRIKFVNSGAEYEVAEVGINSPKPLARDLLMAGDVGYITASIKDIKDARVGDTITAANNPAPEALHGYRQMQPMVYAGLYPTDNAKFNDLREALEKLQLNDASLTFEPESSQALGFGFRCGFLGMLHMDVIQERLEREFNLDLITTAPSVTYHVYQHDGEMIEVENPAEMPDVTLIKSIEEPFVKASIMVPNDYVGAVMELCQRKRGQFDTMEYLSDTRVNVIYHIPLAEIIYDFFDRLKSSTRGYASLDYEIDDYRPSDLVKIDILLNGDRVDALSFISHRSFAEERGREIASKLKKIIPRQNFEIPIQAAIGSKIIARTNIKAYRKDVTARIHTGDPDRRAKLLDKQKRGKKRMKAVGRVEVPQAAFMAVLQTDEEIDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKP8</accession>
    <name>H4GKP8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome-binding factor A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rbfA</name>
        <name evidence="2" type="ORF">PS3_9393</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Binds 30S ribosomal subunits, but not 50S ribosomal subunits or 70S ribosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RbfA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122630.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9393"/>
    <dbReference type="EnsemblBacteria" id="EHS84968">
        <property type="protein sequence ID" value="EHS84968"/>
        <property type="gene ID" value="PS3_9393"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1618"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00003">
        <property type="entry name" value="RbfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000238">
        <property type="entry name" value="RbfA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023799">
        <property type="entry name" value="RbfA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020053">
        <property type="entry name" value="Ribosome-bd_factorA_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33515">
        <property type="entry name" value="PTHR33515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02033">
        <property type="entry name" value="RBFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89919">
        <property type="entry name" value="SSF89919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00082">
        <property type="entry name" value="rbfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01319">
        <property type="entry name" value="RBFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0690">Ribosome biogenesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="120" mass="13756" checksum="499A501847DD67B9" modified="2012-04-18" version="1">MPSKQFRVERLAQQIQREVDDILLKRVRDPRVQGVTITGVDVTGDLQQATIYYSLLSEYASDGQKAQAGLDKAKSLIRSELSSRLNIFKAPQLIFKRDPSIEYGNRIDELLNKLHRENQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKR1</accession>
    <name>H4GKR1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc metalloprotease</fullName>
            <ecNumber evidence="9">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PS3_6470</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the peptidase M50B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122643.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6470"/>
    <dbReference type="EnsemblBacteria" id="EHS84981">
        <property type="protein sequence ID" value="EHS84981"/>
        <property type="gene ID" value="PS3_6470"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1634"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004387">
        <property type="entry name" value="Pept_M50_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42837">
        <property type="entry name" value="PTHR42837"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00054">
        <property type="entry name" value="TIGR00054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="11" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="332"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="191"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS84981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="422" mass="46576" checksum="141529F2533174C5" modified="2012-04-18" version="1">MIITIIAFIIIFGILVLVHEYGHYFFAKRAGILVREFSIGMGPKIWWKRRHGTTYTIRILPLGGYVRMAGMDDDSDSLRPGLAVSLLLNDQGKVTTINASNQTTLFQGVPLRVVDFDLEKDLWISGYLSDDETEVKRYDVDHDATIIEKDGTEVRIAPLDVQFNSASLPARMMTNFAGAMNNFILSLVVFVIVGFMVAAIPTNSNRLGSVVNNSPAAKAGLKANDQITKINSTTVKNWDTMSSYISDQPGKKLQITFVRNGVTHHTSATTKKIVQNGQSYGQLGITEKTTTNFKARLGYGWHMFTYSATLIFTTLGHMFTRGFSLNDLGGPVAIYAGTSQATSLGVTGVLSFLAMLSINLGIVNLLPIPALDGGKLLLNICEAIIRRPIPEKFELAINLIGFAFLMLLMILVTWNDIQRYFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKR7</accession>
    <name>H4GKR7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">30S ribosomal protein S2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpsB</name>
        <name evidence="7" type="ORF">PS3_6375</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122649.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6375"/>
    <dbReference type="EnsemblBacteria" id="EHS84987">
        <property type="protein sequence ID" value="EHS84987"/>
        <property type="gene ID" value="PS3_6375"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1640"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_B">
        <property type="entry name" value="Ribosomal_S2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_S2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_S2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_S2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_S2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="PTHR12534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="SSF52313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01011">
        <property type="entry name" value="rpsB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="224"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="240"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS84987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="257" mass="29004" checksum="0BC0F90579A0AA82" modified="2012-04-18" version="1">MPVVSMKQLLEAGVHFGHQTRRWNPKMAEYIFTERNGIYIIDLQKTVKMVDSAYNYVKDVAANGGIVLFVGTKKQAQDSIEEEAKRAGQYYINHRWLGGTLTNWKTIQSRIKRLKELKEMAEDGTFDRLPKKEVAVLTKQREKLERFLGGIEDMPRIPDVLYVVDPHKEQIAVREAQKLHIPIVAMVDTNSDPDDIDVIIPSNDDAIRAVRLITATMADAVIEGKQGEDDQDAPVEEAGEVSEDSLKDLKDSVEGNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKU6</accession>
    <name>H4GKU6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rqc2 homolog RqcH</fullName>
            <shortName evidence="1">RqcH</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rqcH</name>
        <name evidence="4" type="ORF">PS3_7760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ribosome quality control system (RQC). Recruits Ala-charged tRNA and directs the elongation of stalled nascent chains on 50S ribosomal subunits, leading to non-templated C-terminal Ala extensions (Ala tail). The Ala tail promotes nascent chain degradation. May add between 1 and at least 8 Ala residues. Binds to stalled 50S ribosomal subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Associates with stalled 50S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NEMF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122678.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7760"/>
    <dbReference type="EnsemblBacteria" id="EHS85016">
        <property type="protein sequence ID" value="EHS85016"/>
        <property type="gene ID" value="PS3_7760"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1675"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00844_B">
        <property type="entry name" value="RqcH_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043682">
        <property type="entry name" value="RqcH_bacterial"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="NFACT-R_1" evidence="3">
        <location>
            <begin position="450"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="431"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="294"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="445"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="559" mass="63578" checksum="AFB8F17DA193DFB8" modified="2012-04-18" version="1">MAFDGLFTHAMVHELNATLAGGRIAKINQPYPLELLMVIRANRHNYPLLLSANPAYPRVQITQIPYQNPANPSNFTMTMRKYLDGAIIDEIKQVDNDRIIQIFFQHRDELGDLQTLILQIEIMARHSNIILFQAADHKIIDTIKHVGSDQNRVRTLLPGTTFIMPPKQARTNPFLPNQHYSDLVRQFKDDPRELAKALQNQYQGFGSDSALELAHRLLTSDHLPSTYENFLATFNQTHATLYENERGKLNFAAFTPTINYKHPRSFDSLSDLLDAFYADKAERDRSKELAGQVIKVINNVLKKDRRKVKKLNQELDDAKTADQYRINGEILTTYLHQLQPGMTEIELPNFYANNEPITISLAPELSPSRNAQRYFTKYNKLKTSVDYVNEQLALTNQEIDYLSAILSQIDLAAPADIQDIRLELQEQGYIKQKGSSKKQRKTKPSQPSEYQTSSGIPVLVGKNNRQNDQLTFKIANKNELWFHVKDLPGSHVVLRSTSPSDEEIQEAAEIAAYFSKGRDSSHVQVDYLPIKAIHKPSGARPGFVTFRGQTTLTVTPKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKX2</accession>
    <name>H4GKX2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_21928</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000073">
        <property type="protein sequence ID" value="EHS84946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122704.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000073.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21928"/>
    <dbReference type="EnsemblBacteria" id="EHS84946">
        <property type="protein sequence ID" value="EHS84946"/>
        <property type="gene ID" value="PS3_21928"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1709"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS84946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="367" mass="40809" checksum="5DB1B28F36BABD29" modified="2012-04-18" version="1">MIKEHRQILFWSLELLILAGLLWVGSQLQFVLEPIITFVSAVFVPLIISVFLYYMLSPLMNLIMKLRIGPFKIPRSLASLFIVLLLILLVVGGISALIPSVINEFNQLIQWLPGAAKQGQELINQWSHYKWVKQIDLSAYYDQINQYLTRFAKQAVTTLSSSAGSIIGTITSTVIITITVPVMLFYMLKDSPKLVPSIQKIFSPQRSQEVSDLLHQMNRTLSSYISGQAIECLFVAIATSIGYLIINQPLAIVLGVIAGIANMVPYVGPYIGIAPSLLVSIAIAPNKIIWVIVVVVIVQQIDGNIIYPNIIGRSLKIHPLTIIVLLLAAGNIAGIPGMILCIPFYAVIRTVVIYFWNIWQLQSEKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKX9</accession>
    <name>H4GKX9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">30S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpsG</name>
        <name evidence="10" type="ORF">PS3_14249</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit. Contacts proteins S9 and S11.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000076">
        <property type="protein sequence ID" value="EHS84831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122711.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000076.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14249"/>
    <dbReference type="EnsemblBacteria" id="EHS84831">
        <property type="protein sequence ID" value="EHS84831"/>
        <property type="gene ID" value="PS3_14249"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1720"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00480_B">
        <property type="entry name" value="Ribosomal_S7_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_S5/S7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005717">
        <property type="entry name" value="Ribosomal_S7_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_S7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_S7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_S7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="PTHR11205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="SSF47973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01029">
        <property type="entry name" value="rpsG_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6 8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_S7" evidence="9">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS84831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="156" mass="17851" checksum="CD23B00DA7AD6F4A" modified="2012-04-18" version="1">MPRKGHIAKPEVLPDPIYNSKLVTSLINHLMLDGKRGTASKILYQALDQIKEQTGNDPLEVFEQAMENIMPALEVKARRIGGSNYQVPIEVRPDRQRTLALRWLTQYARLRGEHTMVERLANEIIDASNNTGASIKKKEDTLRMAEANRAFAHYRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKY4</accession>
    <name>H4GKY4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">DNA-directed RNA polymerase subunit beta</fullName>
            <shortName evidence="6">RNAP subunit beta</shortName>
            <ecNumber evidence="6 8">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">RNA polymerase subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Transcriptase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpoB</name>
        <name evidence="16" type="ORF">PS3_7285</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6 8">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6 8">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="6 8"/>
    <dbReference type="EMBL" id="AICN01000076">
        <property type="protein sequence ID" value="EHS84836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122716.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000076.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7285"/>
    <dbReference type="EnsemblBacteria" id="EHS84836">
        <property type="protein sequence ID" value="EHS84836"/>
        <property type="gene ID" value="PS3_7285"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1726"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01321">
        <property type="entry name" value="RNApol_bact_RpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042107">
        <property type="entry name" value="DNA-dir_RNA_pol_bsu_ext_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019462">
        <property type="entry name" value="DNA-dir_RNA_pol_bsu_external_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010243">
        <property type="entry name" value="RNA_pol_bsu_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="PTHR20856"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10385">
        <property type="entry name" value="RNA_pol_Rpb2_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02013">
        <property type="entry name" value="rpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 6 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA_pol_Rpb2_1" evidence="13">
        <location>
            <begin position="31"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_2" evidence="12">
        <location>
            <begin position="143"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_2" evidence="12">
        <location>
            <begin position="380"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_3" evidence="14">
        <location>
            <begin position="478"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_45" evidence="15">
        <location>
            <begin position="556"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_6" evidence="10">
        <location>
            <begin position="684"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_7" evidence="11">
        <location>
            <begin position="1072"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1169"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1176"/>
            <end position="1192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF10385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EHS84836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="1192" mass="133372" checksum="55AD1D5D6252C34E" modified="2012-04-18" version="1">MNSLAGHLVKYGKRRTRRSYSRIREVLDLPNLIEIQTDSYNWFMDTGLREMFNDIMPIDDFQGKLSLEFVDYQLLEPKYTVDEAREHDANYSAPLHVTLRLTNHETGEIKSQDVFFGDFPLMTDQGTFIINGAERVIVSQLVRSPGVYFSQEADKNGRPNFGANFIPNRGAWLEYETDAKGISYVRIDRTRKLPITELVRSLGFGSDDEIIDMLGDESESLNATIDKDVHKEPEDSRVEEALKDIYERLRPGEPKTADSARSLLITRFFDPKRYDMAPVGRYKTNKKLKMKYRLLGQTLAETLADPETGEILANKGDVVTKEVLKNLESALESDDFKMITYTPSDDAVVTEPVQLQKILVYSKDDPEKVVPIVSNGNISLDLKHIQPADIIASVNYFLNLQEGIGTTDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDINTVTPQQLINIRPVVASIREFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVRDVHYTHYGRMCPIETPEGPNIGLINSLSSYARVNQYGFIETPYRRVSWDTHKVTDRIDYLTADEEDNYIIAQANTKLNEDGSFAEEMIMCRDKEDYIETSADNVDYMDVSPKQVVSVASACIPFLENDDSNRALMGANMQRQAVPLINPHAPLIGTGIEYKAAHDSGVALIAKNAGTVEFVDAKEIRIRQEDGSLDTYKLMKFRRSNGGKNYNQRPIVKVGEHIDVDDVLADGPSMEQGELALGQNPLIAFMTWQGYNFEDAIAINERLVKDDVYTSIHIESYESEARETKLGPEEMTREIPNVGQEALRDLDEGGIIRVGAEVNDGDILVGKVTPKGVTELSAEERLLHAIFGEKSREVRDTSLRVPHGGGGIVQDVKVFTRENGDELAPGVDMMVRVYIAQKRKIQVGDKMAGRHGNKGTVSIVVPEEDMPYMPDGTPIDIMLSPMGVPSRMNIGQVIELHLGMAARELGIHIATPVFDGATDADVWDAVKEAGLPSDGKTILYDGRTGEPFEQRVSVGVMHYLKLAHMVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEILTYKSDDVVGRVNTYEAIINGEQIPKPGVPESFRVLVKELQSLGLDMKVLDNDHQEVELKNMDEDVMDVRKMAQKAKPVEATATETSEATDEVPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKY6</accession>
    <name>H4GKY6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ATP-dependent Clp protease ATP-binding subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_9730</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000076">
        <property type="protein sequence ID" value="EHS84838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9730"/>
    <dbReference type="EnsemblBacteria" id="EHS84838">
        <property type="protein sequence ID" value="EHS84838"/>
        <property type="gene ID" value="PS3_9730"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1728"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="6">
        <location>
            <begin position="65"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="6">
        <location>
            <begin position="404"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="ClpB_D2-small" evidence="7">
        <location>
            <begin position="581"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="679" mass="75940" checksum="EBCFBEF28A5C699F" modified="2012-04-18" version="1">MKRLGVSERNMNRSNRQNNQPAEGTPTLDKLGRDMTKLANEKDVDPVIGRNKEIQRALQILSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVKGSVPETLQNKRLISLDMGSLVAGTKFRGEFEDRLKKVINEIAEDGNVILFVDEIHTLIGAGGAEGAIDASNILKPALARGEIQLIGATTLDEYRKYVEKDAALERRFSTVMVEEPSTEETLTILEGLREQYQQYHQVEITDDALQSAVELSKRYINDRYLPDKAIDLMDESAATIRINAENHPHKDIQLSQQLAEVRNQKEQAILEQNFDLAADLRQSELKLKVELEAYEVKRQRAADDGHYRLKVTANDVATVVAEWTGVPVTQLQKQESKRLVNLEQNLHQRVIGQDEAVRVISEAIRRSRSGLKDPQRPIGSFMFLGPTGVGKTELAKALAEEMFGSRDQMIRIDMSEYMERYSTSRLVGAAPGYVGYEEGGQLTEQVRRHPYSVVLLDEVEKAHPDVFNLLLQVLDDGFLTDSQGRRVDFRNTILIMTSNLGATQLQDTKTVGFGATDVHEDYAAMKAAVNEQLKLAFRPEFLNRIDETIVFHSLTKPELRQIVKLLASQLIHRLADQGIKLKLTTAALDTIAEDGYNPAYGARPLRRSIQKLVENPMSMLILNGDIQVGDQITVGAHKGQLDFKQNGQLVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKZ5</accession>
    <name>H4GKZ5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Orotate phosphoribosyltransferase</fullName>
            <shortName evidence="4">OPRT</shortName>
            <shortName evidence="4">OPRTase</shortName>
            <ecNumber evidence="2 4">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pyrE</name>
        <name evidence="6" type="ORF">PS3_17971</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>diphosphate + orotidine 5'-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + orotate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="2 4"/>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122727.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17971"/>
    <dbReference type="EnsemblBacteria" id="EHS84761">
        <property type="protein sequence ID" value="EHS84761"/>
        <property type="gene ID" value="PS3_17971"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1738"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 4" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="5">
        <location>
            <begin position="69"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-phosphoribose 1-diphosphate binding" evidence="4">
        <location>
            <begin position="123"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS84761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="212" mass="23108" checksum="22F8A7E32382145F" modified="2012-04-18" version="1">MTNYAEKVAQGLLDIQAVTLSPDQPFTWASGLHSPIYTDNRLTISYPDVRANIYQGMVKLIKANFPEAEVIAGTATAGIPHAAWVAQAMDLPFIYVRSKPKDHGQGKQVEGVLKSGQKVVVIDDLLSTGGSVLKAAQAVNREGGQVLGVVAVFSYQLPALDQNFAEAGLSYQTVTDYTTLITTAEQKQLINQDQLASLHQWREDPQAWSDQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL01</accession>
    <name>H4GL01_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycerophosphodiester phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_11609</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11609"/>
    <dbReference type="EnsemblBacteria" id="EHS84767">
        <property type="protein sequence ID" value="EHS84767"/>
        <property type="gene ID" value="PS3_11609"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1747"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018476">
        <property type="entry name" value="GlyceroP-diester-Pdiesterase_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10110">
        <property type="entry name" value="GPDPase_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="SSF51695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="2">
        <location>
            <begin position="304"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS84767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="553" mass="63799" checksum="43D9D07399795894" modified="2012-04-18" version="1">MLAWTLSWSVVLQFIIIPVGRCLASQLFYTANWPLVTIANLILVIDHHPWALLGLGLEVLFALALTYWMVTVWFNGLLSISQGEQIKFKTGKIWRPDSWSGISVMLLIALVGLPWVNLLYRTPLWSQIQLPIFLLDFLPRHTWILATATILYLLAVCLMYRWVMVLPQIMLNHLAVRQALQKSWQETKGWAGLQLWRSICLAILWPTLILWLIYGLVWVSPIAQLPRSMMGLVIGGLQIISELALSIIVIRLTLVLVGSRNLKDGNQEPLDWRWGCLFLLVLGLLLGKAIYQGQAVNQMAVKKLLVISHRGVNGHNGVQNTIPALLKTIRTSHPNYVEIDVHETKDGQFVVLHDENLRKLAGINRRPRQLTLRQLEKITVRENGYRAKLVSLDQYLKVAQQHHQKLIVELKTSRYDSSLMVNHFVRRYAKTLIAHHDLVHSLDYQALIQMHHAAPRLSIVDIQGYNLMNPRTSFQAYTMEYGSLNNRFMQQAHAQHQRIFAWTVDSPMMMRRMILMGTDGIVTDRPSELRRVWHQIYKLDHNRLVLWTYLSGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL11</accession>
    <name>H4GL11_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_10998</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122743.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10998"/>
    <dbReference type="EnsemblBacteria" id="EHS84777">
        <property type="protein sequence ID" value="EHS84777"/>
        <property type="gene ID" value="PS3_10998"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1759"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS84777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="298" mass="31470" checksum="53B44BDD509D73C6" modified="2012-04-18" version="1">MSIILSAIGQGLLWGLLGLGLYLTFRILDFPDLTVEGTFPLGACVCVAAITHGLSPFIGLLVSLLAGALAGLATGLLYTKLNIPNILAGILVMTGMYSIDLRILGRSNVGLLNYQTFFNYDFIQQLPDYLGAIVIGVIFVVVVITLLTGFINTDLGQAFVATGDNERMAASFGINTDNTKILGLMVSNGIIGLAGGMIAQHDGYADVNMGIGTIVIGLAAIIIGEVLLPNLSLSGRLIAIIIGSIAYRFVILIVLQLGFNTNDTKLLSAIILALCLAIPVLRHKLKLDHLFNQGGQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL12</accession>
    <name>H4GL12_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_4570</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122744.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4570"/>
    <dbReference type="EnsemblBacteria" id="EHS84778">
        <property type="protein sequence ID" value="EHS84778"/>
        <property type="gene ID" value="PS3_4570"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1760"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS84778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="253" mass="27872" checksum="467F946EE32E45D2" modified="2012-04-18" version="1">MTTLLELNHIETTVNTGTPNERAILKDLNLTVEQGEFITLLGTNGAGKSTLLNVINGSLRPSHGQVVIDQHPVTKLPEAKRAKYIAQVFQDPKLGTAPRMTVAENLLLAAKRGQRRSLIPRRLNAHLDEFKQLTSQLPNGLSDRLTTAAGDLSGGQRQTLSFLMATMTKPQLLLLDEHTAALDPNTSHQLMALTDQVVTQQELTCIMITHQIKDALKYGNRIIILNQGQIVLDVKGPERSDLSEADLLQYFEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL20</accession>
    <name>H4GL20_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YycH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_14590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122752.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14590"/>
    <dbReference type="EnsemblBacteria" id="EHS84786">
        <property type="protein sequence ID" value="EHS84786"/>
        <property type="gene ID" value="PS3_14590"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1770"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009996">
        <property type="entry name" value="YycH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042274">
        <property type="entry name" value="YycH/YycI_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07435">
        <property type="entry name" value="YycH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003591916" description="YycH domain-containing protein" evidence="1">
        <location>
            <begin position="36"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="YycH" evidence="2">
        <location>
            <begin position="15"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS84786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="430" mass="48420" checksum="BF7D8E238CEAA345" modified="2012-04-18" version="1">MIKWMKTNLLPLGVIIAVVISVVLSISVWINPYQANRIPASNSTSSTNDSLTSMGSVYQANQVVVTKQDGSQQLLYNQKRNPALTIQQALTKAKMSGLNRVANGDSGKYLDYLRMANTVTISFPDQISMTVFNQVFDQSLDADRLATVNYVVVPLNDRSRVYLLADQGKKVYRVKVDQLSLKTIKQSLKGGRRVDVDHKIVNGTPILSYPHEYTLPTYSYDVSQHNVDNLAQTLLATANSNTIDTERNGNQIIYTNGNSHRMTYNRQTGSIQYDNYLGKDQKYGRQSLESHLYNRLKKLSIDLTGIYFDTVSQAGQTVTYRSFVQGFPIFSSRNYGTILIKSNNDGMERYQLSNYYLGLPQTSDGTTVTMPSSAVVSNALKESGQLKKVKGMRIGYTWNNDDTETVTLEPTYYVYYNGNWVPYQTLLKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL28</accession>
    <name>H4GL28_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_22336</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122760.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_22336"/>
    <dbReference type="EnsemblBacteria" id="EHS84658">
        <property type="protein sequence ID" value="EHS84658"/>
        <property type="gene ID" value="PS3_22336"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1780"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038766">
        <property type="entry name" value="Membrane_comp_ABC_pdt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30287">
        <property type="entry name" value="PTHR30287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="369"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="415"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="490"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="719"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="775"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="817"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="8">
        <location>
            <begin position="351"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="244"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS84658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="852" mass="95312" checksum="E18C19448403EFB0" modified="2012-04-18" version="1">MSKQILWKDAWQAIPRSFGRFIAISLLMLLGTFAFIGLKITGPDMRQTALDFFKQNNLADVTVTSTYGLDQTDQQTIKDQVGVRQVTFGYYQDAAINHSQTSIRVYSNTKQISKYQLVSGKRPTKANEIALSTSLQGKYKLGQTINLGHTTELKNQKFKVVGFVKSSEYVSRTDYGQTNVGTGQLSGIAVTTKNAFNATNYNIARVTYRATKRMNAYSDRYNEYTDDKKTQLQTALNKLRAPKYQNYQSQITTAQNQVTQLEQAASANPALQTQLSQAQAQLTSTENNLKNLGYPSYTVSTRSSFPGYSVYRSNSRRVDVLANVFPVFLFIIAALVSLTTMMRFVEEERITIGTFSALGYSHRDISLKFILYSIMSSGIGVLLGAIGGFTILPQIVFKAYAASSTISGLQLHFSWTYLLIVIAVALCSTTLAALYALRKDYQEHPASLLLPKPPKAGSKILLERFTPLWRHLSFNYKVTFRNLFRYKSRALMTIFGVAGCVGLLVMGIGIRDSLSGIISKQYETIIKYDLIVAQKAAPIETESQSYQKLLNSDKVRRHQAIYTQQFNKIAGSDQSTQTITVIVPANQKHFNKFVNLLSPSGQELSLPKNGVILTQKMADLLKAKKGSQVSLKDANGQSHHFKVMGIAQMYMGHYLFMSRSAYEQSFKTNYQTNAQLVTLKHNSSKQVKKVAQSFIRTGAVTTTSLNLENQQLIANVINGLNTVIVVLIAIATILAMVVLYNLTNINVSERIRELSTIKVLGFFDREVTMYIYRETIILTTLGILFGYLFGYGLHAFIMINLPPDNAMFDDSMYPLNFLISTIIPCLITLGLAFIMHRKIRDVDMLEALKSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL54</accession>
    <name>H4GL54_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cell division protein FtsW</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_6378</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122786.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6378"/>
    <dbReference type="EnsemblBacteria" id="EHS84684">
        <property type="protein sequence ID" value="EHS84684"/>
        <property type="gene ID" value="PS3_6378"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1810"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018365">
        <property type="entry name" value="Cell_cycle_FtsW-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001182">
        <property type="entry name" value="FtsW/RodA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30474">
        <property type="entry name" value="PTHR30474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01098">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00428">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS84684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="389" mass="42373" checksum="9D5D7D46A87BEFCD" modified="2012-04-18" version="1">MNKQKKRSVLGWDPYLLIPYLILCIIGIVMVYSASADVAMQVGGTARSYLVKQSIFALVGIGIFFLLSRVNLTFWRSKYTLGFLSGILIVLLVYVRFFGAAVNGARGWVNLGPVSIQPAEVCKFFLTIYLANQFARYENLSRSDYLRKLRKPIILTIVLIGLILSQPDTGGALINASIFLVIIMASRIPWKYGATALVGGFLALLIGLPYVAQLFAKIGGYRAARFVAYLNPFANASGSGTQLVNSYYAISNGGIFGSGIGNSIQKMGYLPESNTDFILAIISEELGLVTVLIILALLATLICRIILLGIRSNNLYETLLCYGTGTFFAVETMFNVGAVCGLLPITGVTLPFISYGGSSMFVLSASLGLVANVERRMRRQKARRQQEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL73</accession>
    <name>H4GL73_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Cell division protein FtsA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ftsA</name>
        <name evidence="9" type="ORF">PS3_7786</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Cell division protein that is involved in the assembly of the Z ring. May serve as a membrane anchor for the Z ring.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Self-interacts. Interacts with FtsZ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="5">Localizes to the Z ring in an FtsZ-dependent manner. Targeted to the membrane through a conserved C-terminal amphipathic helix.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the FtsA/MreB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122805.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7786"/>
    <dbReference type="EnsemblBacteria" id="EHS84703">
        <property type="protein sequence ID" value="EHS84703"/>
        <property type="gene ID" value="PS3_7786"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1836"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02033">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020823">
        <property type="entry name" value="Cell_div_FtsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003494">
        <property type="entry name" value="SHS2_FtsA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02491">
        <property type="entry name" value="SHS2_FTSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003101">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00842">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01174">
        <property type="entry name" value="ftsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 5 6" id="KW-0132">Cell division</keyword>
    <keyword evidence="1 5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FtsA" evidence="8">
        <location>
            <begin position="7"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="403"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="423"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR003101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS84703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="462" mass="50284" checksum="208E84A3193F2BC8" modified="2012-04-18" version="1">MQNTQLYVGLDIGTTSIKVLVCENVKGDLKVIGVGTQPTTGLNRGVIVDIDKTASAISQAVAQAAEKSGVQIREVVVGLPANYLRLSEVHGMVTLNTEQGQSREITDQDVIDVAQATMNQALSPDQEPIDLVIRDFAVDRFTGIKDPRGMVGVRLEMWATMYSGLTTVIHNARKAVEQAGLNVQDFVIAPIATGFNLLNDGEQDFGTLMVDLGGGQTTTSIILDHQLKFAYVDPEGGQLITHDISEVLNTSQRNAEQLKRDHGFANSELASDDVQLPVEVVGKNQPVNISEKYLSEIIEARVRQIFERAKNQLDSVQAPDLPGGLVLTGGVAALPGIKELASEYFSGNIKVFVPDQMGIRHPGFSQVLALCRYESNLSEVEQLVKLVVNQGALIESPHQYGQFQNVPEPQYRPPVAPTPRRAASEGDNNQSDAPRPSRPNQRKSSSNPTIEKVKGFFSNFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL81</accession>
    <name>H4GL81_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA translocase FtsK</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PS3_15384</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Homohexamer. Forms a ring that surrounds DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FtsK/SpoIIIE/SftA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122813.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15384"/>
    <dbReference type="EnsemblBacteria" id="EHS84711">
        <property type="protein sequence ID" value="EHS84711"/>
        <property type="gene ID" value="PS3_15384"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1847"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041027">
        <property type="entry name" value="FtsK_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002543">
        <property type="entry name" value="FtsK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018541">
        <property type="entry name" value="Ftsk_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17854">
        <property type="entry name" value="FtsK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09397">
        <property type="entry name" value="FtsK_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01580">
        <property type="entry name" value="FtsK_SpoIIIE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00843">
        <property type="entry name" value="Ftsk_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50901">
        <property type="entry name" value="FTSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="11" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="FtsK" evidence="10">
        <location>
            <begin position="452"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="207"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="765"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="226"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="269"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="470"/>
            <end position="477"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS84711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="785" mass="86777" checksum="02414162652D6153" modified="2012-04-18" version="1">MAAKRTTSRKRTASPRRRRTNASARQRDQRIIFSLSGILVILLTLLGLFRLGLIGQWVYDLFMILGGQSYFVLMLVLIIYCLGLSIYGKFPHFKKRLIIGFGLFYLGLVTYLHYLMFNQIDQAQHIFNTTWNNILQATLGGDNTLFVGGGMIGAMCYQITSYLIAPIGNAILGWLLMLVGVVVFFNLPWNKVLVGVQNLFHWLKDQGERRKNQTSQSHSKPGQKQVKKRAKESDKRTSLLDKFKDRRQDPAEKDLPTPVSERPPIVEPTINVATSTNPTKPSENLQNSQTNTPDVETSDEGHEGVVLAGVEGSTVDDDYQLPPANLLAKVQSTDQSADLNSIKANTKKLQETLASFGVDATVENVNLGPTVTKYELRPAIGVKVSKITHLADDLALALAAKDIRIEAPIPGKSLIGIEVPNTKIATVGFRDMIEAAPRNDKPLEVPLGRTVTGTVMMADLTKMPHLLIAGSTGSGKSVAINGIIVSILMKAKPNQVKFLMIDPKKVELSIYNGIPHLLSPVVSEPKKAARALGKVVAEMERRYELFSAFNVRNLDGYNQMVRESGEADQHELPLILVIVDELADLMMTVSNDVETAIVRIAQMGRAAGIHMILATQRPSVDVITGLIKANVPSRIAFAVSSGIDSRTILDANGAEKLLGRGDMLFQPIDQNKPTRVQGAFISDHDVESVVDFIKDESQVEYDDRIVVTDNEMEQAENAEDEDELFQEALAFVVDQQKASTSLIQRRFRIGYNRAARIIDDLEQRGYIGPANGSKPREVYKQADHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL89</accession>
    <name>H4GL89_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Competence negative regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_3990</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MecA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122821.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3990"/>
    <dbReference type="EnsemblBacteria" id="EHS84719">
        <property type="protein sequence ID" value="EHS84719"/>
        <property type="gene ID" value="PS3_3990"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1856"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038471">
        <property type="entry name" value="MecA_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008681">
        <property type="entry name" value="Neg-reg_MecA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39161">
        <property type="entry name" value="PTHR39161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05389">
        <property type="entry name" value="MecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029008">
        <property type="entry name" value="MecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS84719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="233" mass="27065" checksum="DC18DBA820EECAA3" modified="2012-04-18" version="1">MMEVNRLDDDTLQVIISQDDLEARGITMLDLMGDQHQIQEFFYQVLEEVDTENQFKDNDSVTFQVLQSPSGIELIISKNNSFLDNPELLQQHQEQLSKFLKKELQAHDRKTTISDHDDSSAEQTDEIESALNDPDLQKYTKVVSFADFEDFIELSRLADTSNLASDLYKYQDHYYLILTFFENGDLTSEAIRDRLGIVYEYANESTMDPMLLREHGQHVMTQAAFELARHYFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL92</accession>
    <name>H4GL92_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ion transport 2 domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PS3_14039</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000079">
        <property type="protein sequence ID" value="EHS84621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122824.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000079.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14039"/>
    <dbReference type="EnsemblBacteria" id="EHS84621">
        <property type="protein sequence ID" value="EHS84621"/>
        <property type="gene ID" value="PS3_14039"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1860"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="PTHR11537"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="184"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Ion_trans_2" evidence="10">
        <location>
            <begin position="135"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="212"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS84621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="248" mass="28449" checksum="22C0F060863DCECB" modified="2012-04-18" version="1">MINNKYKNIIYIGYEIMMAALSIASIFMIVLDYAKSIDITAYPYNVFDNLILTIFTIDYFGRLIASKNKRKFIAGNIWDLLSIIPVNQIFYFFRVARVFRIFRLLKLLRLVRLIGLTGRLRKFINTNGLIYYIYISIVVLIIGASMYSISEKVDFATALWWSITTATTVGYGDISPTTSIGKLAAVMVMIIGIGFIGMLTSSISNFFIANDDVDLKDELDKLHKENDQLNDKLDKLEQIIREQQQNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHQ4</accession>
    <name>H4GHQ4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein translocase subunit SecA</fullName>
            <ecNumber evidence="11">7.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">secA</name>
        <name evidence="15" type="ORF">PS3_11354</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer and homodimer. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF. Other proteins may also be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="11">Distribution is 50-50.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the SecA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.4.2.8" evidence="11"/>
    <dbReference type="EMBL" id="AICN01000002">
        <property type="protein sequence ID" value="EHS87559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11354"/>
    <dbReference type="EnsemblBacteria" id="EHS87559">
        <property type="protein sequence ID" value="EHS87559"/>
        <property type="gene ID" value="PS3_11354"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.6"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017038">
        <property type="term" value="P:protein import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18803">
        <property type="entry name" value="SF2_C_secA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01382">
        <property type="entry name" value="SecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000185">
        <property type="entry name" value="SecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022490">
        <property type="entry name" value="SecA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020937">
        <property type="entry name" value="SecA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011115">
        <property type="entry name" value="SecA_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014018">
        <property type="entry name" value="SecA_motor_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011130">
        <property type="entry name" value="SecA_preprotein_X-link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044722">
        <property type="entry name" value="SecA_SF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011116">
        <property type="entry name" value="SecA_Wing/Scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036266">
        <property type="entry name" value="SecA_Wing/Scaffold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036670">
        <property type="entry name" value="SecA_X-link_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612">
        <property type="entry name" value="PTHR30612"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07517">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01043">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07516">
        <property type="entry name" value="SecA_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00906">
        <property type="entry name" value="SECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00957">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00958">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81767">
        <property type="entry name" value="SSF81767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81886">
        <property type="entry name" value="SSF81886"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03714">
        <property type="entry name" value="secA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01312">
        <property type="entry name" value="SECA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51196">
        <property type="entry name" value="SECA_MOTOR_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-1278">Translocase</keyword>
    <keyword evidence="9 11" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SECA_MOTOR_DEAD" evidence="14">
        <location>
            <begin position="1"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="80"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="413"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="96"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="491"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EHS87559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="791" mass="90465" checksum="34CC198E606AB321" modified="2012-04-18" version="1">MSIIDRFELSRIRRILKQVNSYADQMAAMTDGQLKDQTRKFKARLAKGETLDDLLPEAFATVREADKRLLGMFPFDVQVMGAIALHQGAVAEMKTGEGKTLTATLPIYLNALSGEGAMLITPNEYLARRDGEEMGVVYQWLGLTVAVGFPKTSDKEWKPAQKRAVYNSDVIYTTNGTIGFDYLIDNLASDEEHKYMRPFNYAIVDEADAVLLDSAVTPLVISGAPRVSSTFIGVADEIIYTLIRDVDYEMDEEEDNVWLTNQGIDKIENFMQIDDLFDGHHKDQVRSIELALKAHFLFEKDEDYVINQDNEVSLMDKGNGRVMQGMKLQAGQHQAIEMKEQVDVTDDTRAMATITYQNLFRMFKKLSGMTGTGKVANQEFIESYFMKVIQIPTRKPVIRKDYPDQIYTTLPEKLQASLEEVKQIHALGRPILLVTATVELSEIYSELLLREHIPHSVLNARNAAKEAMIIKEAGQLGAVTVATNMAGRGTDIKLGPGVKELGGLAVIGTEKMASKRIDQQLRGRAGRQGDPGSSKFFISLEDEVVIKYGAHWVHRYYDRHESKRSMNAPKRLRRGKFRRVINYAQEDSDASGAQQRAQSLEMDVSSQIQRELVYQQRNDIIFGEGQVDFDPEQLFRDEFNLFFTEHQPLTMDQLDRYILDNITYQYFDAPQGIDLANRKQVVDFLMQLADEEMTAKKQEFATEEEQDRFIRLSLLKAIDECWIDEVDGLEQLRQIVVARQFAQRQPTFEYHKEAERAYRQMKKEIRHMVLRYLLLSTIITDKKGKKDIYYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHR0</accession>
    <name>H4GHR0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_13439</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000003">
        <property type="protein sequence ID" value="EHS87555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121594.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000003.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13439"/>
    <dbReference type="EnsemblBacteria" id="EHS87555">
        <property type="protein sequence ID" value="EHS87555"/>
        <property type="gene ID" value="PS3_13439"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.22"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009370">
        <property type="entry name" value="YutD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038141">
        <property type="entry name" value="YutD-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06265">
        <property type="entry name" value="DUF1027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF012565">
        <property type="entry name" value="DUF1027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="137"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="106"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR012565-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="184" mass="22083" checksum="8161346D7A5A3A95" modified="2012-04-18" version="1">MKRDVVQAYIDQRAAQRAGVYQVVRDDESHFKINGHQYLLIKDYRAGFDPEQLANRFSSILSKYDYIVGDWGHNQLRLRGFYQSDNPLFDRDHSVTLIQDYLYEECNFGCPYFIIQNLEVAIPRRVVNQKRSAHALKEYREHQRSASRPKPRYRKNQEVTSVKNKHRPNRRKFVIRDKKKRVHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHR5</accession>
    <name>H4GHR5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Mannose-6-phosphate isomerase</fullName>
            <ecNumber evidence="3 4">5.3.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_3546</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>D-mannose 6-phosphate = D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12356"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="5.3.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4 7">Belongs to the mannose-6-phosphate isomerase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.8" evidence="3 4"/>
    <dbReference type="EMBL" id="AICN01000004">
        <property type="protein sequence ID" value="EHS87545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121599.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000004.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3546"/>
    <dbReference type="EnsemblBacteria" id="EHS87545">
        <property type="protein sequence ID" value="EHS87545"/>
        <property type="gene ID" value="PS3_3546"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.28"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004476">
        <property type="term" value="F:mannose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001250">
        <property type="entry name" value="Man6P_Isoase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014628">
        <property type="entry name" value="Man6P_isomerase_Firm_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01238">
        <property type="entry name" value="PMI_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036894">
        <property type="entry name" value="PMI_Firm_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00218">
        <property type="entry name" value="manA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <feature type="active site" evidence="6">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036894-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR036894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="318" mass="35756" checksum="04881288656FA822" modified="2012-04-18" version="1">MNDVLFLKPAFQTKMWGGQKLRTDFHYDIPSDNTGECWAISAHPHGPATIANGDFSGLTLAELWDQHPDFFGNPSGPVFPLLTKILDAAKILSVQVHPDDQYAQEHEHELGKTECWYVLSAEPGSYLIYGHNARDKDELKNLVDQGQWDQLLRKVYVKTGDFFFVPSGTLHAIGPGVVVLETQQSSDTTYRFYDFDRVDPATGKTRELHLQQSIDCTETPFVDQNQTPVTTKYDHASITQLVDSTHFIVNKWQTNFGHLTLHHEVAPYTLASVVDGQGELKINDQVYPITKGDHFIIPATTDKWTLDGNMTVIASEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHT5</accession>
    <name>H4GHT5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 5">Thymidylate synthase</fullName>
            <shortName evidence="5">TS</shortName>
            <shortName evidence="5">TSase</shortName>
            <ecNumber evidence="1 5">2.1.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">thyA</name>
        <name evidence="8" type="ORF">PS3_10530</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8-dihydrofolate + dTMP</text>
            <dbReference type="Rhea" id="RHEA:12104"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.45" evidence="1 5"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121619.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10530"/>
    <dbReference type="EnsemblBacteria" id="EHS87491">
        <property type="protein sequence ID" value="EHS87491"/>
        <property type="gene ID" value="PS3_10530"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.54"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00008">
        <property type="entry name" value="Thymidy_synth_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000398">
        <property type="entry name" value="Thymidylate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020940">
        <property type="entry name" value="Thymidylate_synthase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="PTHR11548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00108">
        <property type="entry name" value="THYMDSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="SSF55831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03284">
        <property type="entry name" value="thym_sym"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00091">
        <property type="entry name" value="THYMIDYLATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4 5" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylat_synt" evidence="7">
        <location>
            <begin position="8"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="181"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="221"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="262"/>
            <end position="264"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="319" mass="36852" checksum="59350B85D90FF9C3" modified="2012-04-18" version="1">MSINDNEQQYLDFARYVLEHGDEKGDRTGTGTRSVFGYQMRFDLQAGFPILTTKRVPFGLIKSELLWFLRGDTNIRFLLQHKNHIWDEWAFQRWVESDEYTGPDMTDFGRRSLVDADFNQVYQEQKRQFCERILNDDEFSAKYGDLGLVYGSQWRAWQSPKGPIDQIQNVIDQIKTNPNSRRLIVSAWNPVDVPSMALPPCHTMFQFYVNNGKLSCQLYQRSADIFLGVPFNIASYALLTHMIANQCGLEVGEFVHTFGDAHIYSNHIEQIKEQLSRPMHQAPRLILPDEAKPVDEYEMSDIKLEGYEAEPAIKAPVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHT6</accession>
    <name>H4GHT6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_11327</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121620.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11327"/>
    <dbReference type="EnsemblBacteria" id="EHS87492">
        <property type="protein sequence ID" value="EHS87492"/>
        <property type="gene ID" value="PS3_11327"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.55"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032524">
        <property type="entry name" value="ABC_tran_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16326">
        <property type="entry name" value="ABC_tran_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="4"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="320"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="532"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="569"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="532"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="352"/>
            <end position="359"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="630" mass="71713" checksum="C59A6D4920ABFF69" modified="2012-04-18" version="1">MQTLRADNLTSTYGEKTLFKDVSFIINENDRIGLIGTNGSGKTSLLNVIAGLTNAEQGAITKPSEYTIGYLQQQPNLDPQKTVTEAVFASDQAVFKLIRRYHQALDEFSQHPEDSQAIEAYTKLQAQMDQANAWEAESQIKTILTQLHLTDLNRKISELSGGQLKRVGLAVVLIQHPDLLILDEPTNHLDLDSIIWLQDYLAAYKGAVLIVTHDRYFLDQVTNHIWELSFGDLYHYDGNYQAFVEQKANRIELARETEKKRQSLYQKELAWMRTGAKARSTKQKGRINRFNELKSQIGHLQEDQDLEIALGSQRLGKDVIEFKNANLAFDQLTILQDFNWLVQANDRIGISGENGAGKTTLFKVIMGQLPLDSGVLKIGETVKLGYYSQQTEPIPEDKRVIEYLSEVAESVVDRNGQRIEVTGLLERFLFPRFMHGTLIRKLSGGEKRRLFLLKILMQQPNVLLLDEPTNDLDIGTLAVLEDYLDTFAGTVITISHDRYFLDRVAEQLLIFKGQGKIDRYVGRFTDYLNQSAEASSKPTEQSRVNPAPQATPKATKDKTKLTYGERLEWDQLNEDIDQLTDQQQALELAMSENGDNYEKLARLQQELNEVVKTLDEKTARWEYLSEFVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHU8</accession>
    <name>H4GHU8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_21026</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CvfB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121632.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21026"/>
    <dbReference type="EnsemblBacteria" id="EHS87504">
        <property type="protein sequence ID" value="EHS87504"/>
        <property type="gene ID" value="PS3_21026"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.72"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014464">
        <property type="entry name" value="CvfB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039566">
        <property type="entry name" value="CvfB_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040764">
        <property type="entry name" value="CvfB_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37296">
        <property type="entry name" value="PTHR37296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17783">
        <property type="entry name" value="CvfB_WH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13509">
        <property type="entry name" value="S1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF012524">
        <property type="entry name" value="YitL_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1_2" evidence="2">
        <location>
            <begin position="5"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="CvfB_WH" evidence="3">
        <location>
            <begin position="229"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR012524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="299" mass="33508" checksum="BE889A134DC8B163" modified="2012-04-18" version="1">MNTDLGKIVTAVMIDENETDYFVQPARNGQTYRLPKDEAPQVLHVGGQVQGFVYENDHHQLQMTCDHIPTVQIDHYDFGTVVNVRRDLGVFVDIGLPNKDVVVSLDDLPTLKHLWPQPGDRLLIALTVDKHDRIWGKLADEAIFQAIARRPQAKMKNWNVEGTVYRLKMAGSYVLTDDYCLGFIHPSERDQEPRLGSKVSARVIGLSSHGVLNLSLKPRSYEAIGEDAQQIMMILEHDPNHELALTDKSDPETIKTILGISKGQFKRAIGHLMKAGLVRQTDGVTKLISDDNGTQDLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHV1</accession>
    <name>H4GHV1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_9715</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Responsible for synthesis of pseudouridine from uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a uridine in RNA = a pseudouridine in RNA</text>
            <dbReference type="Rhea" id="RHEA:48348"/>
            <dbReference type="Rhea" id="RHEA-COMP:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:12069"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121635.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9715"/>
    <dbReference type="EnsemblBacteria" id="EHS87507">
        <property type="protein sequence ID" value="EHS87507"/>
        <property type="gene ID" value="PS3_9715"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.76"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140098">
        <property type="term" value="F:catalytic activity, acting on RNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034470">
        <property type="term" value="P:ncRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluC/D_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PseudoU_synth_2" evidence="4">
        <location>
            <begin position="90"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="287" mass="32936" checksum="3A09821E37949A83" modified="2012-04-18" version="1">MTYSITEISNQPAQPLRQLLQTSWQLPKRYVHFLRINQYVLVNGQYVPMNTIIYPGDQLQMQFSENEFRTRQSYLPNNHQTLPILFENRDLVVINKPAGLKSHPNQPNELDTVMNYMAAQIAHPQMIHRLDQSTSGAMIIGKHPIVVPIINRLLSTHQINREYLAVVSGKWEENAGMINLLIGHDPNDKRKRQVNGIDALPAQTNFKLIQAHQTHSLIKLQLATGRTHQIRVHLKAKHHPIISDPLYGMETGPRMMLHAFQLHLILPFSGEKLTINAPLPAEFEQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHY1</accession>
    <name>H4GHY1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-acetylmuramoyl-L-alanine amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_15349</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121665.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15349"/>
    <dbReference type="EnsemblBacteria" id="EHS87537">
        <property type="protein sequence ID" value="EHS87537"/>
        <property type="gene ID" value="PS3_15349"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.108"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008745">
        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02696">
        <property type="entry name" value="MurNAc-LAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002508">
        <property type="entry name" value="MurNAc-LAA_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003646">
        <property type="entry name" value="SH3-like_bac-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01520">
        <property type="entry name" value="Amidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00646">
        <property type="entry name" value="Ami_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00287">
        <property type="entry name" value="SH3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="SH3b" evidence="2">
        <location>
            <begin position="42"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="MurNAc-LAA" evidence="3">
        <location>
            <begin position="180"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="294" mass="33143" checksum="2CC13DA99A06EBBE" modified="2012-04-18" version="1">MQNRLTFFQKYHHRLPGWIISLVAVFIALGIVISLTNPSLGQQTVQINPKTMTVRQGPGMNYQSINLSHSTSGQVIKTTNGWKQVQLSSGKQVWIAGWLLQAQNLPQKTNLTGATIVIDPGHGGNDTGATYVTNSQNPAYFEKTYTFQLAKKVAQALRRQGARVYLTRNKDEYVSLGARTRLAERVHADLFISFHFDSSPSENEASGFTTYYYHRKNHSRAIAQAINKAFNNLPLTNRGVDFGDFYVLRDNKQPAVLLEMGYINSSKDYQQIKSANYQQKIVTDLILGLKNYFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHZ7</accession>
    <name>H4GHZ7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dihydroorotase</fullName>
            <shortName evidence="5">DHOase</shortName>
            <ecNumber evidence="5">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pyrC</name>
        <name evidence="7" type="ORF">PS3_14258</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(S)-dihydroorotate + H2O = H(+) + N-carbamoyl-L-aspartate</text>
            <dbReference type="Rhea" id="RHEA:24296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="EC" id="3.5.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class I DHOase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000006">
        <property type="protein sequence ID" value="EHS87476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121681.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000006.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14258"/>
    <dbReference type="EnsemblBacteria" id="EHS87476">
        <property type="protein sequence ID" value="EHS87476"/>
        <property type="gene ID" value="PS3_14258"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.127"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01317">
        <property type="entry name" value="DHOase_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00220_B">
        <property type="entry name" value="PyrC_classI_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004722">
        <property type="entry name" value="DHOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="6">
        <location>
            <begin position="52"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="62"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="428" mass="46023" checksum="ECFE6D5C826E94DE" modified="2012-04-18" version="1">MQTLIQHGQVYTGGHLVNTDILIKDGRIQALGHDLIDPQNVDTVIDAEKLLVSPGLIDVHVHYRDPGLTQKETIKTGSMAAAHGGFTTVGAMPNVDPVPDTPDKIAHMVERNQSEGVVHIKQYASITMGRLSDELVDYAGVKDAGAFALSNDGNGVQLAGTMYRAMQGAAKAGLPIAAHVEDESLLFGGKMTLGERSQELGIPGAPSVSESAQVARDLVLANDTGVQYHICHISTKESLAMVKFAKEKGVPVTCEASPHHLLLSDEEIHGENANYKMNPPLRHADDRAALQSSLMDGTVDMIATDHAPHTADDKPNDFSCAANGITGSETAFAECYTHLVKQGSLSLERLLDLMATNPADIFHLEGAGRIEVGQPADLAIFDLNHEYEIKESDYLSKGKNTPFTGDHVYGRTIYTLVDGQIVYQAKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI41</accession>
    <name>H4GI41_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glucokinase</fullName>
            <ecNumber evidence="2">2.7.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Glucose kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_6173</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.2" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121725.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6173"/>
    <dbReference type="EnsemblBacteria" id="EHS87380">
        <property type="protein sequence ID" value="EHS87380"/>
        <property type="gene ID" value="PS3_6173"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.184"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004654">
        <property type="entry name" value="ROK_glcA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00744">
        <property type="entry name" value="ROK_glcA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01125">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="322" mass="33926" checksum="011E6142027E3601" modified="2012-04-18" version="1">MSDKKLIGVDLGGTTIKFAILTDDGKIQQKWSIRTNVLDEGSHIVPDIIESINHHIDLYKMDKSQFVGIGMGTPGTVNRQLGTVIGAYNLNWKTTQPVKEQIEAGTGLKFAIDNDANCAGLGEAWKGAGNDGDDVVFITLGTGVGGGIVANGELVHGINGAAGEVGHMCVEPNNGYQCTCGNYGCLEQYASATGIVHLAQDATEEYVGNSPLKEMVDNGEEVTSKDVFEIAQTGDYLANQVVDKVCFYLGLACANISNTLNPESLVIGGGVSAAGAYLLDRVKNNFEKYAFPTVRTSTVLKLAQLGNDAGVIGAASLAKQFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI46</accession>
    <name>H4GI46_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Gluconate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_11862</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121730.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11862"/>
    <dbReference type="EnsemblBacteria" id="EHS87385">
        <property type="protein sequence ID" value="EHS87385"/>
        <property type="gene ID" value="PS3_11862"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.191"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015128">
        <property type="term" value="F:gluconate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003474">
        <property type="entry name" value="Glcn_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30354">
        <property type="entry name" value="PTHR30354"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02447">
        <property type="entry name" value="GntP_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002746">
        <property type="entry name" value="Gluconate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00791">
        <property type="entry name" value="gntP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="307"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="343"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="383"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="447" mass="47807" checksum="498ADB650818E592" modified="2012-04-18" version="1">MPLLIVLIGVIILIFLIVKLKLNTFVSLVVTSFIVALLLQIPVTKIPTTIESGIGSQLGHLAIIFGFGSMLGSLVSEAGGGYRIATTLINKFGRRWIQVAVILASFIIGLALFFEVGLVVVLPIIFFIARELDMPLMYLGVPMAATLNVTHGFLPPHPAPTAITEVLHAQLGHVLLLGIVVAIPTIIIAGPLYNWALQKLYPKVYRKNIDISVLGEYKEFKLADTPGFGISVLTAMMPVILIAIATICFFVLPSKSLVNQVIQFIGAPDFAMLLSMLIAVFTMGIFRQKNMKEISDIMVSSVKSISMMLLIIGGGGAFKQVLVDGGISKYVSALFTSTNLSPIFAAWLITAILRVSLGSSTVSGITAAGLLAPMAEQYAPGSAMPALMVLAVGAGSIFCDHVNDAGFWMIKEYFGLSLKETLLSWTTLTIVISLAGLGAVYTLSLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI51</accession>
    <name>H4GI51_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="13">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="13">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">pheS</name>
        <name evidence="15" type="ORF">PS3_14274</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="13"/>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121735.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14274"/>
    <dbReference type="EnsemblBacteria" id="EHS87390">
        <property type="protein sequence ID" value="EHS87390"/>
        <property type="gene ID" value="PS3_14274"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.196"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00281">
        <property type="entry name" value="Phe_tRNA_synth_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004530">
        <property type="entry name" value="Phe-tRNA-synth_IIc_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004188">
        <property type="entry name" value="Phe-tRNA_ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022911">
        <property type="entry name" value="Phe_tRNA_ligase_alpha1_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF41">
        <property type="entry name" value="PTHR11538:SF41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02912">
        <property type="entry name" value="Phe_tRNA-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13 15" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="14">
        <location>
            <begin position="112"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EHS87390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="348" mass="39090" checksum="6ED89EBBB3A1AEAB" modified="2012-04-18" version="1">MSLKDQLTELRAQALEAIQNSTDDQAINEARVKFLGKKGPITAVLRGMKDLSKEERPKVGQFANEIRDQIQVALDEKRSAIEAARLNQQLASETLDVTLPGRPVIQGQPHVIQQIIDQIIDLFVGMGYEVATGHEVEEEVYNFEKLNLPKDHPARDMQDTFYITPSILMRTQTSPMQARMMEKHDFSRGPLKMISPGKVYRRDTDDATHSHQFHQVEGIVIGEHITMADLKGTLEAVAQNLFGDELSVRLRPSYFPFTEPSVEADITCFNCHGQGCSICKQTGWIEALGAGMVHPNVLKMSGVDPEVYGGFAFGLGPDRFAMLKYGVEDIRNFYLNDMRFLTQFDQKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI58</accession>
    <name>H4GI58_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal transduction histidine-protein kinase ArlS</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PS3_19579</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121742.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19579"/>
    <dbReference type="EnsemblBacteria" id="EHS87397">
        <property type="protein sequence ID" value="EHS87397"/>
        <property type="gene ID" value="PS3_19579"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.210"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041610">
        <property type="entry name" value="ArlS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18719">
        <property type="entry name" value="ArlS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="11">
        <location>
            <begin position="203"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="10">
        <location>
            <begin position="265"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="485" mass="55332" checksum="CA253E97CDE4A4C6" modified="2012-04-18" version="1">MENQASTKRFLSLKLKWALGTAVGSWLISILLIVIIFSSFTQDLLRQERQTLSSTMTSVSEQINNHLDTLSSQQIKQEINSEVGSTSSKAVFSRDIVRGLSNSQIEMAIYNQQGKVIYQLGKVQVKFKTSNQRTISLMRGNDHQILVGRMPIRSKDHQVIGYLQIENNLNTYHQRYQRQLLVVVIALLLVVILSGLMGYFLSWFFLRPLNDIEDTVKAISDDPTKDVRVPELRQNDELSELSTMFNQMLDRTQRYIDQQSQFVGDVSHELRTPVAIIQGHMQLLDRWGKDDPQVLADSIQASLKETERMNNLVQEMLDLSRAEQVELNFRDATTPVKEVVMQVYNNFKMIHPDYTFVMDDDLPGEVISPIYHDHLEQIMIILCDNAVKYSTNRPEVDFSLSRSANFVEIGIQDYGEGIADEDIDKVFDRFYRVDKARSRKKGGNGLGLAIAKRLIEGYHGMITLESTLGSGSVFTVKLPIIDEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI62</accession>
    <name>H4GI62_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransf_25 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_20884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121746.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20884"/>
    <dbReference type="EnsemblBacteria" id="EHS87401">
        <property type="protein sequence ID" value="EHS87401"/>
        <property type="gene ID" value="PS3_20884"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.215"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="36"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="245" mass="28477" checksum="DBE2C727929D15B0" modified="2012-04-18" version="1">MIYQTFARLYDELFDDDMYQAWADFVQTIVPQPTAILDVAGGAGRLAVLLAQRGYEMTDLDLSPEMLSLAVEHREAAQVDLMLVEGNMLALGDLPKYPVITCFADSLCYLDNFAEFQQALSEIYAHLEDGGHFIFDMMSPYQMDQVYPGYMFNYQDEDHQRAFMWQSFADDDVDHGVIHDLTFFVQSNNGQYDRISEMHFQRSYPREWVLKTLEKLGYQKIQVRADFGKAAVDEHTTRWFFICQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI66</accession>
    <name>H4GI66_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_9002</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121750.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9002"/>
    <dbReference type="EnsemblBacteria" id="EHS87405">
        <property type="protein sequence ID" value="EHS87405"/>
        <property type="gene ID" value="PS3_9002"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.219"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017924">
        <property type="entry name" value="RNA-binding_YhbY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="SSF75471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00253">
        <property type="entry name" value="RNA_bind_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRM" evidence="3">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="103" mass="11574" checksum="F5BC0F261DCFFD9F" modified="2012-04-18" version="1">MHLRGKQKRFLRAQANHLQPLSSVGKNGLTPAWLAQLSGALDKRELIKVSILQNADETTSEVKAYIESQSDIQVVQKIGRVLVLFKVAKDPELRQLSEQVEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI74</accession>
    <name>H4GI74_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dephospho-CoA kinase</fullName>
            <ecNumber evidence="1">2.7.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dephosphocoenzyme A kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">coaE</name>
        <name evidence="2" type="ORF">PS3_20020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3'-dephospho-CoA + ATP = ADP + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18245"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CoaE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.24" evidence="1"/>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121758.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20020"/>
    <dbReference type="EnsemblBacteria" id="EHS87413">
        <property type="protein sequence ID" value="EHS87413"/>
        <property type="gene ID" value="PS3_20020"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.228"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00356"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00376">
        <property type="entry name" value="Dephospho_CoA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00152">
        <property type="entry name" value="TIGR00152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="197" mass="21731" checksum="C1806DE4D25E577E" modified="2012-04-18" version="1">MTKVIGLTGGIATGKSTVSQYLRTKGLKIIDADIVARQVVEPGTKGLALIQEAFGKEVINPDGSLNRAQLGKVVFADSQARQKLNQILHPLIFRTILGRFEELKAQRCPVVVLDIPLLFETGWEQRCDLTWVVACSPQQQLTRLMARDHLDQAAAQARIAAQYPLTEKMARADVVLMNDNNQSGLYHQVDQALAKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI83</accession>
    <name>H4GI83_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histidine triad protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_12673</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121767.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12673"/>
    <dbReference type="EnsemblBacteria" id="EHS87422">
        <property type="protein sequence ID" value="EHS87422"/>
        <property type="gene ID" value="PS3_12673"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.239"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01277">
        <property type="entry name" value="HINT_subgroup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039384">
        <property type="entry name" value="HINT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648">
        <property type="entry name" value="PTHR46648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="2">
        <location>
            <begin position="5"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="1">
        <location>
            <begin position="98"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="142" mass="16276" checksum="D9A4E9F81ECCD428" modified="2012-04-18" version="1">MEDCIFCKIIAGEIPSYTVYEDDVVKAFLDISQNTPGHTLVIPKKHVKDIFEYDEDLAAEVFKRIPKIARAIKASNPDIKGMNVMNNNGEVAYQSVFHSHWHLLPRYSSDDEFKINFTDHSNDYASEDYEAVRQSIAAQFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI85</accession>
    <name>H4GI85_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Foldase protein PrsA</fullName>
            <ecNumber evidence="11">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">prsA</name>
        <name evidence="16" type="ORF">PS3_17739</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Plays a major role in protein secretion by helping the post-translocational extracellular folding of several secreted proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the PrsA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="11"/>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121769.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17739"/>
    <dbReference type="EnsemblBacteria" id="EHS87424">
        <property type="protein sequence ID" value="EHS87424"/>
        <property type="gene ID" value="PS3_17739"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.243"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01145">
        <property type="entry name" value="Foldase_PrsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023059">
        <property type="entry name" value="Foldase_PrsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00639">
        <property type="entry name" value="Rotamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="SSF109998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50198">
        <property type="entry name" value="PPIC_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12" id="KW-0697">Rotamase</keyword>
    <keyword evidence="6 11 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007915276" description="Foldase protein PrsA" evidence="14">
        <location>
            <begin position="29"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="PpiC" evidence="15">
        <location>
            <begin position="138"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="295"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EHS87424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="334" mass="35557" checksum="7841F9E866259107" modified="2012-04-18" version="1">MKSKKILALVAGVALALPLAACGNKAVATTNGGKITQSEYYSSMKQTTAGKQVLQQMILDKVLEKQYGSKVSSSDVNKEYNAAKKTYGSSFSSVLAQQGYTTSTFKDQIRSRLLLEEAVRASSSFSTSALKKQWKEYQPKISTAMITVGSESDAQSIIDDLNSTSSNKLKEFKKLAKSKSTDSATASSGGVMPTFDNTDTSLPDAVKTAAYKLKNGEYTTSPVQGDDDTYYVIYMIKHPAKGKYANHISDLKDQIVTENMNDSDFVKKVVSKALKKGDVSIKDSDLKNILADYLNTSSSSSSSSSSSSSSSTSSSSASDSSSTESSSASSSSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIB9</accession>
    <name>H4GIB9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">6-phospho-beta-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_18965</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121803.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18965"/>
    <dbReference type="EnsemblBacteria" id="EHS87340">
        <property type="protein sequence ID" value="EHS87340"/>
        <property type="gene ID" value="PS3_18965"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.293"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018120">
        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="PTHR10353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00572">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="496" mass="56943" checksum="264EA31C68BE356E" modified="2012-04-18" version="1">MENNKKLPEGFLWGGATADFQYEGGYDEGGRGLSTHDYETNGSVENPRHHTMEMPDGTIIGPASSFIDAETVPDEAKPVFIDGQYYPSHKAVDFYHHYKEDIKLMAGMGFNVYRFSISWSRIFPTGEEETPNEAGLKFYDDVINEMAKYKMEPLITICHDEMPMNLALKYNGWASRELIDLYVKYAKVLFERYGDRCRYWLTFNEINAVRGFGPTGVRKNQGIDRYQAAHNMFVASAKTVIMGHEMIPNSKFSTMYALSTFYPATCKPQDVFHNMQQLRENWYFVDTMGRGYYPSYADDILLHHGIEGLSEIEMATEDEEILNKGQLDFISFSYYRSNITKDGDDWFNVGGSSNPYLKETPWGWGLDPLGLRYTMNMIYDRIQKPIFIVENGIGIIDQADENGYVEDDERINYLQDHLSAMADAINQDHVPCLGYTMWGPIDLVSLSTGEMKKRYGFVYVDMDDLGNGTLKRTPKKSYYWMKKVIESNGESLADHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIC1</accession>
    <name>H4GIC1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">L-ribulose 5-phosphate 4-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_20426</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121805.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20426"/>
    <dbReference type="EnsemblBacteria" id="EHS87342">
        <property type="protein sequence ID" value="EHS87342"/>
        <property type="gene ID" value="PS3_20426"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.296"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="SSF53639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldolase_II" evidence="1">
        <location>
            <begin position="7"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="242" mass="27184" checksum="C7EEBB4303888DA7" modified="2012-04-18" version="1">MLEELKKEVYDANMQLPELGLVTFTWGNVSGIDREKGLFVIKPSGVDYNDLKPEDMVVVNLKGEVVEGDYNPSSDTPTHTYLYNHFPKIGGIVHTHSPWAVSFAAAKLDIPAMNTTHADTFYNDIPAADALTKEEIEEDYEGNTGKTIVKTFEERGLDYEATPGTLVSQHGPFTWGPTPAKAVYNAKVLEVVAEEDYHTMQMTMDNTRLPQYLLDKHYYRKHGANAYYGQNSAHSKDHAKHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GID7</accession>
    <name>H4GID7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">6-phosphogluconate dehydrogenase, decarboxylating</fullName>
            <ecNumber evidence="4 7">1.1.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_9402</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>6-phospho-D-gluconate + NADP(+) = CO2 + D-ribulose 5-phosphate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:10116"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="1.1.1.44"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 7">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the 6-phosphogluconate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.44" evidence="4 7"/>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121821.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9402"/>
    <dbReference type="EnsemblBacteria" id="EHS87358">
        <property type="protein sequence ID" value="EHS87358"/>
        <property type="gene ID" value="PS3_9402"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.327"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00410"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004616">
        <property type="term" value="F:phosphogluconate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019521">
        <property type="term" value="P:D-gluconate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016054">
        <property type="term" value="P:organic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006114">
        <property type="entry name" value="6PGDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006113">
        <property type="entry name" value="6PGDH_Gnd/GntZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006184">
        <property type="entry name" value="6PGdom_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006183">
        <property type="entry name" value="Pgluconate_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11811">
        <property type="entry name" value="PTHR11811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00393">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000109">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00076">
        <property type="entry name" value="6PGDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01350">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00873">
        <property type="entry name" value="gnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00461">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0311">Gluconate utilization</keyword>
    <keyword evidence="4 7" id="KW-0521">NADP</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 7" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6PGD" evidence="8">
        <location>
            <begin position="180"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="129"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="187"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="453"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="478" mass="53514" checksum="70578CD457D75785" modified="2012-04-18" version="1">MSEEAQIGVIGLAVMGKNLALNIESRGFTVGVYNRHRNRTDEMMKDHSEKKLVPSYTIEDFVDSLEKPRRILMMVQAGKGTDAVIDELLPLLDKGDVLIDGGNTNFHDTMARNAKLDESGINFIGMGVSGGELGALQGPSLMPGGQKEAYDLVEPILTQIAAKAEDGKPCVTYIGPNGAGHYVKMVHNGIEYGDEELIDEAYNVMRNVLGLSVDDMADIFADWNKGELDSYLVEITADILTRKDDLGDDKTKPIVDMILDRGANKGTGKWSSETALDGGAPQSVITEAVYARYISMLKDERVAASKALPEDVESANFTDEEKQEYIEKVREALYFGKVMSYAQGFEQMRIDSERYDWNLQYGELAQIWREGCIIRAQFLQKITDAFEKDPELKNLLLDDYFKEITKKYQKAIRDVVALAVKAGIPVPAFAAAISYYDSYRAEVLPANLLQAQRDYFGAHTYERTDRDGNYHYSWYEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIE1</accession>
    <name>H4GIE1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Oligoendopeptidase F</fullName>
            <ecNumber evidence="6">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_4089</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Has oligopeptidase activity and degrades a variety of small bioactive peptides.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M3B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121825.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4089"/>
    <dbReference type="EnsemblBacteria" id="EHS87362">
        <property type="protein sequence ID" value="EHS87362"/>
        <property type="gene ID" value="PS3_4089"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.335"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013647">
        <property type="entry name" value="OligopepF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042088">
        <property type="entry name" value="OligoPept_F_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004438">
        <property type="entry name" value="Peptidase_M3B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PTHR11804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08439">
        <property type="entry name" value="Peptidase_M3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00181">
        <property type="entry name" value="pepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M3_N" evidence="9">
        <location>
            <begin position="116"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M3" evidence="8">
        <location>
            <begin position="206"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="605" mass="68533" checksum="6D3A8C5308DB68FA" modified="2012-04-18" version="1">MPSTHLPLRKDVPNELKWDLSLLYSDDQAMQADLAEITKLTAQLGDMKGHLGDSAQALTQVLQASRDLDVKLEKAYVYAFLRRDSDTTDARANNLFGQAAQTLVQVQTQTAFIEPELLALPEDKLANWLTTSPALQEFAVEIKRMRLQRGHVLKTSEEALLNRLNRTLDSAEEIYNTLNDADLKFGQIKDEHGQWVELTHGNRSQYTESMDRSVRQAAVKAYAKPYHDLRNTFAQTFNSFVSSQNAQAEIRHYQSARQAALTPHQIDEQVYDTLVTTVNDHLDLAHRWYAIKQRTLGISDFSQADFNVPLAGNEVMKTTFSQGKELTLAALAVLGDDYVKGLQTEFNQRWIDVAENKGKRSGGYQVSVYDGNPFILLNWTDKLYSTFVLAHESGHAMHSWFSQHHQPAQYADAPIFLAEVASTFNENILTEYLLAKYQDDPQIQLYVLEESINGYIGSIFRQTQFAEFEHQAYQAEQDGQTLTADYLDQLSTELLHRYYGPQVSLLAGGASQSWAYVPHFYMNYYVYQYATSWAASSSLAQQVWAGQTGALDRYLGFLKSGSSKDPIETMQAAGIDMTGRAYLDQALKMFAERLDQIEDLLKHQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIK7</accession>
    <name>H4GIK7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Enoyl-[acyl-carrier-protein] reductase [NADH]</fullName>
            <ecNumber evidence="6">1.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_6960</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:10240"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.9" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121891.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6960"/>
    <dbReference type="EnsemblBacteria" id="EHS87202">
        <property type="protein sequence ID" value="EHS87202"/>
        <property type="gene ID" value="PS3_6960"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.418"/>
    <dbReference type="UniPathway" id="UPA00544"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004318">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016631">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05372">
        <property type="entry name" value="ENR_SDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014358">
        <property type="entry name" value="Enoyl-ACP_Rdtase_NADH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43159">
        <property type="entry name" value="PTHR43159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000094">
        <property type="entry name" value="Enoyl-ACP_rdct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 6 9" id="KW-0520">NAD</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="20"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="190"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000094-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000094-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000094-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="252" mass="27215" checksum="B56D23DFABB5AD6B" modified="2012-04-18" version="1">MSGILENKKIVIMGVANKRSIAWGCAKVMAEQGAELIYTYNRDRTLKSIKKLVDDDNRLIQCDASDEASIQTAFNTILDRFGKVDGIVHSIAFANQEDLGGEIMDVTEEGYNLAQDVSAYSLIAVARNGRKILNDPASIVAMSYFGAERAIPNYNVMGVAKAALEANVRYLARDMGKYGVRVNAISAGAIKTLAVTGVQGHKDLLRMSQERTVDGKDATQEEVGGTCAFLMSDLSTGVVGDVIYVDKGVHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIL2</accession>
    <name>H4GIL2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Biotin carboxyl carrier protein of acetyl-CoA carboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_15370</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121896.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15370"/>
    <dbReference type="EnsemblBacteria" id="EHS87207">
        <property type="protein sequence ID" value="EHS87207"/>
        <property type="gene ID" value="PS3_15370"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.423"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009317">
        <property type="term" value="C:acetyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001249">
        <property type="entry name" value="AcCoA_biotinCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01071">
        <property type="entry name" value="ACOABIOTINCC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00531">
        <property type="entry name" value="BCCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0092">Biotin</keyword>
    <keyword evidence="4" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="6">
        <location>
            <begin position="71"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="39"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="149" mass="16094" checksum="D720413AA0F0593B" modified="2012-04-18" version="1">MEFKDIQKLMTAFEKSTTREMDLADGDFHLYLSKNEQSIRSVEPSTTSPAVSAPSTKGAEETPAEPVKSAGQLIKSPLVGTVYLQPKPDTPAYVEVGSQVKVGDVVCIVEAMKMMTEIKSEVAGTISEINVQNGDLVEVEQPLFTVTEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIQ9</accession>
    <name>H4GIQ9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA small subunit methyltransferase A</fullName>
            <ecNumber evidence="6">2.1.1.182</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">16S rRNA dimethyladenosine transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">16S rRNA dimethylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rsmA</name>
        <name evidence="6" type="synonym">ksgA</name>
        <name evidence="9" type="ORF">PS3_13445</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>adenosine(1518)/adenosine(1519) in 16S rRNA + 4 S-adenosyl-L-methionine = 4 H(+) + N(6)-dimethyladenosine(1518)/N(6)-dimethyladenosine(1519) in 16S rRNA + 4 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:19609"/>
            <dbReference type="Rhea" id="RHEA-COMP:10232"/>
            <dbReference type="Rhea" id="RHEA-COMP:10233"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74493"/>
            <dbReference type="EC" id="2.1.1.182"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.182" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121943.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13445"/>
    <dbReference type="EnsemblBacteria" id="EHS87254">
        <property type="protein sequence ID" value="EHS87254"/>
        <property type="gene ID" value="PS3_13445"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.479"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052908">
        <property type="term" value="F:16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00607">
        <property type="entry name" value="16SrRNA_methyltr_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="PTHR11727"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="rADc" evidence="8">
        <location>
            <begin position="38"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="297" mass="33290" checksum="A14A978E003A0761" modified="2012-04-18" version="1">MKKTTAIGNYRRTREILEKHGIHAKKGFGQNFLTDLNVLNGIVQSAHITQGDNVIEIGPGIGALTEVLVKAAHQVVALEIDGDLLPVLDDVLAPYNNVKVIHQDILKANLPELIQTEFTNPQQPIKVVANLPYYITSPILMNLLASEVNWDTICVMMQKEVAERLTAKPGTKAYGSLTLAIEYQMQAKVAFNVSRRSFVPAPNVDSAIVVLTPRQNEIPELPFDDKRLFGLIRGCFAHRRKSLWNNLKSLMSNDRAKLDLVTNALAEVRIDPQTRPEQLTLNDYIRLTNALHQQMVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIR9</accession>
    <name>H4GIR9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NADH peroxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_19585</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121953.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19585"/>
    <dbReference type="EnsemblBacteria" id="EHS87264">
        <property type="protein sequence ID" value="EHS87264"/>
        <property type="gene ID" value="PS3_19585"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.489"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="4">
        <location>
            <begin position="5"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="3">
        <location>
            <begin position="339"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="458" mass="50310" checksum="4FB8F0BEB83C205C" modified="2012-04-18" version="1">MENQKVVIVGASHGGHESAIEILDKYDHVDVVIYEAGDFVSFMSCGMQLFLEDKVTAQDDVRNFAPADIESRGGHVFNQAEVTAINADQKTVTVHFKATDSDQTVPYDKLILSSGVTPMTLPVPGADLDNIYLMRGYDWATKIKAALTDDRFHKITVVGAGYIGVEAADVLSSYGHNKEVTLLDRNDHILERYLNTENIAILTNEFEKHGVKIKTGLNIESFVGENGQVTAIQTDQETIPTDLVIVSAGVKPNTQWLKGTINLDRHGFIQTDAYLRTNLPDVYAIGDAILPLSIPANKPLPIALATTARREAQYVVNHLFEAKPTHPFKGVVGSSALSVYDYHFATAGLNEMTARESHIDFATSFYEDRQRPAYIPKNDNPRVYVSLTYNPLTHQLLGGSVLSTADLTAQGNVLSLAIAQKLTLEDLACADFFFQPGFDRQWSLLNLAAQHALGNAPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIS6</accession>
    <name>H4GIS6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Diacetyl reductase [(S)-acetoin forming]</fullName>
            <ecNumber evidence="4">1.1.1.304</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Acetoin(diacetyl) reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Meso-2,3-butanediol dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_20024</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the irreversible reduction of 2,3-butanediol to (S)-acetoin in the presence of NADH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-acetoin + NAD(+) = diacetyl + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:27286"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15687"/>
            <dbReference type="ChEBI" id="CHEBI:16583"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.304"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.304" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121960.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20024"/>
    <dbReference type="EnsemblBacteria" id="EHS87156">
        <property type="protein sequence ID" value="EHS87156"/>
        <property type="gene ID" value="PS3_20024"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.503"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052588">
        <property type="term" value="F:diacetyl reductase ((S)-acetoin forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045150">
        <property type="term" value="P:acetoin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014007">
        <property type="entry name" value="23BDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02415">
        <property type="entry name" value="23BDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="257" mass="26825" checksum="750C6BE71F44A8DD" modified="2012-04-18" version="1">MSKKVALVTGAGQGIGKAIAERLAKDGFAVAVLARHADKLNEVVAEIEANGGEAFAVTADVAKRDEVFAAVDQVVDHYGDLNVMVNNAGVAPTTPIDTVTPDDLDYVYGINVGGTIWGIQAAHAAFKKLGHPGKIINATSQAGVVGNPNLTVYSSSKFAIRGITQVVARELAEEGTTVNAFAPGIVKTPMMYDIAHQVGKNAGKSDEWGMQTFAKDIALGRLSEPEEVASAVAFLAGPDSDYITGQTLEVDGGMQFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIS9</accession>
    <name>H4GIS9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">50S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">rplM</name>
        <name evidence="7" type="ORF">PS3_14602</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 6">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121963.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14602"/>
    <dbReference type="EnsemblBacteria" id="EHS87159">
        <property type="protein sequence ID" value="EHS87159"/>
        <property type="gene ID" value="PS3_14602"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.508"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_L13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_L13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_L13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_L13_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="PTHR11545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="SSF52161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01066">
        <property type="entry name" value="rplM_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="147" mass="16409" checksum="9122C4C01648F12A" modified="2012-04-18" version="1">MRTTYMAKPGEVDRKWYIVDATDVPLGRLASVVASILRGKNKPTFTPHVDTGDNVIVINAAQVKLTGKKLSQKMYYRHSNHPGGLKKRSAAEYLALAPERMVEKTIKGMLPHNPLGRKTGMKLHVYADANHKHEAQKPEVLDITNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIV0</accession>
    <name>H4GIV0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">50S ribosomal protein L29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpmC</name>
        <name evidence="6" type="ORF">PS3_15360</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121983.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15360"/>
    <dbReference type="EnsemblBacteria" id="EHS87180">
        <property type="protein sequence ID" value="EHS87180"/>
        <property type="gene ID" value="PS3_15360"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.532"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_L29/L35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_L29/L35_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018254">
        <property type="entry name" value="Ribosomal_L29_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="SSF46561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00579">
        <property type="entry name" value="RIBOSOMAL_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="68" mass="8005" checksum="C6B2F9E930CF3C4C" modified="2012-04-18" version="1">MKTKDYVQELNGLTTDQLQNREKELKEQLFNLRFQLATGQLENTASLKTVRKDIARVKTVLRQQELNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIV3</accession>
    <name>H4GIV3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8 11">50S ribosomal protein L22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rplV</name>
        <name evidence="12" type="ORF">PS3_9726</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="7">This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="8 11">This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g., L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8 10">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121986.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9726"/>
    <dbReference type="EnsemblBacteria" id="EHS87183">
        <property type="protein sequence ID" value="EHS87183"/>
        <property type="gene ID" value="PS3_9726"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.535"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_B">
        <property type="entry name" value="Ribosomal_L22_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_L22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_L22/L17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_L22/L17_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005727">
        <property type="entry name" value="Ribosomal_L22_bac/chlpt-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501">
        <property type="entry name" value="PTHR13501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="SSF54843"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01044">
        <property type="entry name" value="rplV_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 8 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8 10" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="115" mass="12477" checksum="77627385076C709E" modified="2012-04-18" version="1">MAENVTSAKATAKMVRVSSWKVRLVLDTIRNKTVAEAFAILKFTPNGAASDVEKVLKSAVANAENNFDLDQASLVVSECYANEGPTLKRFRPRAKGSASPINKRTSHITVVVTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIW6</accession>
    <name>H4GIW6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pyrimidine-nucleoside phosphorylase</fullName>
            <ecNumber evidence="8">2.4.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PS3_8251</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes phosphorolysis of the pyrimidine nucleosides uridine, thymidine and 2'-deoxyuridine with the formation of the corresponding pyrimidine base and ribose-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2'-deoxyuridine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:22824"/>
            <dbReference type="ChEBI" id="CHEBI:16450"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate + thymidine = 2-deoxy-alpha-D-ribose 1-phosphate + thymine</text>
            <dbReference type="Rhea" id="RHEA:16037"/>
            <dbReference type="ChEBI" id="CHEBI:17748"/>
            <dbReference type="ChEBI" id="CHEBI:17821"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>phosphate + uridine = alpha-D-ribose 1-phosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:24388"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.2" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121999.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8251"/>
    <dbReference type="EnsemblBacteria" id="EHS87138">
        <property type="protein sequence ID" value="EHS87138"/>
        <property type="gene ID" value="PS3_8251"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.551"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004645">
        <property type="term" value="F:1,4-alpha-oligoglucan phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016154">
        <property type="term" value="F:pyrimidine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006206">
        <property type="term" value="P:pyrimidine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006213">
        <property type="term" value="P:pyrimidine nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036566">
        <property type="entry name" value="PYNP-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013102">
        <property type="entry name" value="PYNP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018090">
        <property type="entry name" value="Pyrmidine_PPas_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017872">
        <property type="entry name" value="Pyrmidine_PPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000053">
        <property type="entry name" value="Thymidine/pyrmidine_PPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10515">
        <property type="entry name" value="PTHR10515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07831">
        <property type="entry name" value="PYNP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000478">
        <property type="entry name" value="TP_PyNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00941">
        <property type="entry name" value="PYNP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="SSF47648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="SSF52418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54680">
        <property type="entry name" value="SSF54680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02644">
        <property type="entry name" value="Y_phosphoryl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00647">
        <property type="entry name" value="THYMID_PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="PYNP_C" evidence="14">
        <location>
            <begin position="345"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00003877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00011889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00014680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EHS87138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="432" mass="45879" checksum="973014D3CB24F775" modified="2012-04-18" version="1">MRMLDVIDRKRNGHQLCDQELAFFVQGVVEGTIPDYQISALLMAIYFQGMTDEEQASLTMKMLASGDQLDLSAISGVKVDKHSTGGVGDKTSLPLAAMVAALGIPVPMISGRGLGHTGGTLDKLEAIPGYRIDLSEAEFIDQVARDGLAIVGATGEVAPTDKKIYALRDVTDTVDSIPLIASSIMSKKIASGTDALVIDVKTGAGAFMKTLDDSRALAKALVTIGQQVGLDCKAIISDMNQPLGNKIGNALEIEETIDLLKGQGPADLLELVLTLGSYMAVMGGKATDSEQGRAKLLQTIEDGSALAKFKQLVIDQGGDARVIEDYTVMPQAKYKIAYQAKRSGVLAKLTADEIGKAAMVLGGGRQQAEDQLDYAVGIELHKKLGDPVETGEPILTIYSNQPEIQDALALLDDGIEISDQVEVPDLIHEVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIX0</accession>
    <name>H4GIX0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_17930</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122003.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17930"/>
    <dbReference type="EnsemblBacteria" id="EHS87142">
        <property type="protein sequence ID" value="EHS87142"/>
        <property type="gene ID" value="PS3_17930"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.562"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008788">
        <property type="term" value="F:alpha,alpha-phosphotrehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005993">
        <property type="term" value="P:trehalose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032091">
        <property type="entry name" value="Malt_amylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012769">
        <property type="entry name" value="Trehalose_TreC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16657">
        <property type="entry name" value="Malt_amylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02403">
        <property type="entry name" value="trehalose_treC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aamy" evidence="2">
        <location>
            <begin position="14"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="560" mass="64864" checksum="392DE93450E503A1" modified="2012-04-18" version="1">MTRQTDLGKKVIYQLYPKSFYDSNGDGIGDLRGVIQKIDYLKQLHIDQIWFNPFFISPQHDNGHDIADYYRIDPKFGTMDDFREMVKVLKEAHIGVMLDMVLNHISTDHEWFQRALAWDAKYQKFFYLRSPKANGDLPNNWESKFGGPAWAPFGDSGQYYLCLYDKTQADLDWHNPEVRQELEKVVRFWMQEGVEGLRFDVINVIGKDEQLVDSPSPRQLTVEKALYTDTPVVHQYLKELNQKTFGADDQIVTVGEMSSSTIENSIEYTNPSEHELDMVFTFHHLKVDYQDGNKWTDMPFDFQKLKHLLIDWQTKMDQGHGWNALFWNNHDQPWALSRFGDSGQYREKSAEMLAAAMHLLRGTPYIYMGEEIGSINTPYQSIDEYNDVESINAYHQLMAEGKSPQEALHLVYLKSRDNSRVPLAWDDSPQAGFTTGTPWLQPKNQERINVKAELDHGEIFNFYQRLIQLRKSEPLISAGHFRAHLADHPAVLAYERYLDDDQLLVFNNFYGDATEVLVPSEFVGKGGQVLIDNYEADLAQLPGQIKLRPYESLAFKISRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIY0</accession>
    <name>H4GIY0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
            <shortName evidence="7">EPSP synthase</shortName>
            <shortName evidence="7">EPSPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">aroA</name>
        <name evidence="9" type="ORF">PS3_6154</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.19" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122013.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6154"/>
    <dbReference type="EnsemblBacteria" id="EHS87152">
        <property type="protein sequence ID" value="EHS87152"/>
        <property type="gene ID" value="PS3_6154"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.579"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00089"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000505">
        <property type="entry name" value="EPSPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01356">
        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00104">
        <property type="entry name" value="EPSP_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00885">
        <property type="entry name" value="EPSP_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EPSP_synthase" evidence="8">
        <location>
            <begin position="7"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate-3-phosphate binding" evidence="7">
        <location>
            <begin position="21"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoenolpyruvate" evidence="7">
        <location>
            <begin position="91"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="342"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="432" mass="45917" checksum="B77CB0A16E0CA506" modified="2012-04-18" version="1">MKILPTAPQGLQGTLQVPGDKSISHRALMIGSISEGETQIENFLTGEDCLSTLQAFRDLGVQIEQTGTQVTIHGVGLRGLKAASHPLDMGNSGTTTRLMMGLLAGQPFESHLTGDDSLQKRPMKRVAEPLSQFGGRIDLSPAGTLPATVIGQPLHATNYQLKVASAQVKSALILAALQADGPSTIKELLPTRNHTEIMLRQFGAEIETASDGLTITVQPQPKLRGQSVVVPSDISSAAFFLVAGAITPNSTITLTNVNLNPTRTGILQVLQKMQADLRITSKPSDGEPLGDLTIRTSDLAPIEIGAADIPAVIDELPLVALLAARAQGQSVIRGAEELRVKETDRIATVVAELRKLGVTVEELPDGMIIQGRPAWDIQDHQLDSYGDHRLGMMNAIAALTADEQLTLANEAAIKVSYPTFFADLDHLLGGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIY3</accession>
    <name>H4GIY3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Triosephosphate isomerase</fullName>
            <shortName evidence="4">TIM</shortName>
            <shortName evidence="4">TPI</shortName>
            <ecNumber evidence="4 5">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tpiA</name>
        <name evidence="6" type="ORF">PS3_4781</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="4 5"/>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122016.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4781"/>
    <dbReference type="EnsemblBacteria" id="EHS87155">
        <property type="protein sequence ID" value="EHS87155"/>
        <property type="gene ID" value="PS3_4781"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.582"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="PTHR21139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="SSF51351"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 4 5" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="2 4 5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="3 4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="active site" description="Electrophile" evidence="4">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="261" mass="28815" checksum="A50F0E01C1D39874" modified="2012-04-18" version="1">MRKPFVAANWKMHKNVQESVDFINQIKGKLPSFDQLEVGIAGTTLALHSMAVAAKDSDVQIIAQNAFKEFEGAYTGEISPKALADSGVDYVLLGHIERRTMFNESNQMVNQKVIATLQAGMTPIICTDETMVQKEHGGSVHYVFAQLMNVLHDVSFDQVKNIIISYEPSWAVGAGQHANPILAEEGCRQIRQTIADSYSYEVADKIRILYGGSVNPDNIEELMRKRDIDGVLIGRASLDADNFLSMVNTVNNLNASPKVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIY6</accession>
    <name>H4GIY6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Arginine biosynthesis bifunctional protein ArgJ</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="7">Glutamate N-acetyltransferase</fullName>
                <ecNumber evidence="7">2.3.1.35</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="7">Ornithine acetyltransferase</fullName>
                <shortName evidence="7">OATase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="7">Ornithine transacetylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="7">Amino-acid acetyltransferase</fullName>
                <ecNumber evidence="7">2.3.1.1</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="7">N-acetylglutamate synthase</fullName>
                <shortName evidence="7">AGSase</shortName>
            </alternativeName>
        </domain>
        <component>
            <recommendedName>
                <fullName evidence="7">Arginine biosynthesis bifunctional protein ArgJ alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="7">Arginine biosynthesis bifunctional protein ArgJ beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="7" type="primary">argJ</name>
        <name evidence="8" type="ORF">PS3_11326</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>L-glutamate + N(2)-acetyl-L-ornithine = L-ornithine + N-acetyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:15349"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.3.1.35"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Heterotetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">Some bacteria possess a monofunctional ArgJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ArgJ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.35" evidence="7"/>
    <dbReference type="EC" id="2.3.1.1" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000025">
        <property type="protein sequence ID" value="EHS87129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122019.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000025.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11326"/>
    <dbReference type="MEROPS" id="T05.002"/>
    <dbReference type="EnsemblBacteria" id="EHS87129">
        <property type="protein sequence ID" value="EHS87129"/>
        <property type="gene ID" value="PS3_11326"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.587"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00106"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:acetyl-CoA:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004358">
        <property type="term" value="F:glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103045">
        <property type="term" value="F:methione N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02152">
        <property type="entry name" value="OAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01106">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002813">
        <property type="entry name" value="Arg_biosynth_ArgJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042195">
        <property type="entry name" value="ArgJ_beta_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100">
        <property type="entry name" value="PTHR23100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01960">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="SSF56266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00120">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_5023265343" description="Arginine biosynthesis bifunctional protein ArgJ alpha chain" evidence="7">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023265342" description="Arginine biosynthesis bifunctional protein ArgJ beta chain" evidence="7">
        <location>
            <begin position="181"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="7">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="7">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="7">
        <location>
            <begin position="180"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="394" mass="42071" checksum="4F2E2AAE3C30DE17" modified="2012-04-18" version="1">MQEIDFKWPLGFKSDGIHIGLRKSKLDFGWIVSEVPASAAGAYTTNKFCAAPTALTKDAINHGHQLQAIVMNSAIANSCTGQQGQSDAFEEQSLVAQKLGIESTLVGVASTGLIGAFLPMDKVKVGIQTLNQTNNFKVTEAVLTTDTHPKTISLEFEIAGQTCTMSGFCKGSGMIHPKMATMLGFITTDATINGDLLQTILSEQVDLTFNQITVDGDTSTNDMVVVMANGQAKAPIIQDSSEDYESFKTALHQVMTYLAQQIAKDGEGATKLVEANVSQAPSPFDAQKIAKAVVGSNLVKAALFGEDANWGRIISTIGITDADFNPATIDIALNGHPVVQQSLGLNFDEATLKQALQANKITIDIDLHDGQATGQAWGCDLTYQYVQINASYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIZ0</accession>
    <name>H4GIZ0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-dehydropantoate 2-reductase</fullName>
            <ecNumber evidence="5">1.1.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ketopantoate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_3009</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5">Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>(R)-pantoate + NADP(+) = 2-dehydropantoate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:16233"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.169"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 5">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.169" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122023.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3009"/>
    <dbReference type="EnsemblBacteria" id="EHS87102">
        <property type="protein sequence ID" value="EHS87102"/>
        <property type="gene ID" value="PS3_3009"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.594"/>
    <dbReference type="UniPathway" id="UPA00028">
        <property type="reaction ID" value="UER00004"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ApbA" evidence="6">
        <location>
            <begin position="6"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="ApbA_C" evidence="7">
        <location>
            <begin position="188"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="321" mass="36280" checksum="793B49C4BC4028EB" modified="2012-04-18" version="1">MTLKYTVLGAGAMGLRFGVLLQEHAGAEVEFVDNWQPQVDTIKEQGGVYVSRDHENKHLVPVKIYSPEEYNGDPDVFIVFAKQMTLQDLLDRSARFFHPDKQYVFSGMNGMGHVEKINQYFDPSHVLAGTCLIGTVLNQAGDVDFIGKSGAGSINVAKESDDFDQATLDQIMTDFTAANLNPTLHDNFYGTLMTKVVFNSVINTLCTLFEIQMGQFIDYEGAEKLGVQLIDEAFDVCERAGIQLLSTRQEEWETVRYVSAVSNPLHYPSMYQDMSKGRNTEVDYINGYIYDLGKKYNYEAKTHDFLRGLVHLAENTRKFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIZ4</accession>
    <name>H4GIZ4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_8613</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122027.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8613"/>
    <dbReference type="EnsemblBacteria" id="EHS87106">
        <property type="protein sequence ID" value="EHS87106"/>
        <property type="gene ID" value="PS3_8613"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.598"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="310"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="363"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="425"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="447"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="475" mass="50570" checksum="FEE2FA4B55110FC1" modified="2012-04-18" version="1">MSLWKTMTRQEDPSVYQDKDGHLKRTLTTKDFIGLGVGTIVSTAIFTLPGEVAALHAGPSVSLSFLLAAVVAGLVAFAYSEMAAAMPFAGSAYSWINVVFGEFFGWVAGWALLAEYIIAVAFVGSGFSANFRPLLATWGLKLPASLSNPFGTDGGVIDLCSALVIILVALLVSRGVSGATKVENIMVTLKVLAVLTFIFVGATALHASYYDPFIPAYKETANGAFGGWQGIYAGVSSIFLSYIGFDSIAANSAEAKNPGKTMPRGILGSLAIAVVLFVAVSLVLVGMVPYTKLNTAEPVGMALRAAGHPAVAAVVQTIAVIGMFTALLGMTLAGSRLTYSFGRDGMLPKWLGKLDKRNNPNNALLTITVLTVVIGAFFPFAFLSQLISAGTLIAFMFVSLGIYALRRREGKDIPVPEFKMPWYPVMPALAFVASLIVFWGLANDAKIYAGIWFVLGVIIYFAYGRTHSYLAKRDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIZ5</accession>
    <name>H4GIZ5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_20224</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122028.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20224"/>
    <dbReference type="EnsemblBacteria" id="EHS87107">
        <property type="protein sequence ID" value="EHS87107"/>
        <property type="gene ID" value="PS3_20224"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.599"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07208">
        <property type="entry name" value="Pat_hypo_Ecoli_yjju_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037483">
        <property type="entry name" value="YjjU-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="3">
        <location>
            <begin position="7"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="38"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="159"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="285" mass="31912" checksum="4326AEBEBA376791" modified="2012-04-18" version="1">MLYNAALVLEGGAMRGQYSAGVTDAFLKHHLEFKTVIGVSAGALCGANFVSKQYGRMVHVNTHYRHDPNYISSLNIIKKQPVVNLDFLFDDHGWDWNNFDEEAYQRSATDFTIVATSLAGKTVSFTNPLGEKLVEALKASSSMPFLFDPQPTDQGLCLDGGVTDSIPFDLAQAKGFDKIVVVRTRPRDYQKKATSPLLKRLYQRHYRDYPDFITAAINRPEVYNQQVATLNQLEDKGQIYVIAPENPVKVGRLESNVKKIQALYDEGQAQAEALISEIEEYLAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ05</accession>
    <name>H4GJ05_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_2951</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122038.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2951"/>
    <dbReference type="EnsemblBacteria" id="EHS87117">
        <property type="protein sequence ID" value="EHS87117"/>
        <property type="gene ID" value="PS3_2951"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.618"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amoino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="406"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="AA_permease" evidence="7">
        <location>
            <begin position="19"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="458" mass="50225" checksum="47A5E29D6B454B92" modified="2012-04-18" version="1">MEESKPKLSRTMTPGQMEMIAIGGTIGSGLFMGATSTIKWTGPSVLLAYAFVGIILYAVMRALGEMVYLNPGTGSFADYGSKYINPFAGYLVKWSNVFQFIIVGISDIIAMSEYLNYWWPNLPDWLSGIVMIIILTLANLVSAKAYGQLESWFAMIKVATIIVMIILGLLVITIGLGNNWHPVGISNLWSHGGFFTGGLTGFLFSLSVIAGSYQGVELLGITAGESASPQKAIVKSVRSVIWRILIFYIGAIFVIVSIYPWNQLSSVGSPFVQTFTKVGITGAAGIINFVVLTAALSGANSGIYSASRMLFKLSVDGEVPAYFKRLSKRIVPNVAIITIAFWIFAGFIVNYILTAVSSGTANLFVMVYSSSVLPGMVPWFVILLSEHNFRKQHPERLIDHPFQMPLFPGLNYFALISLTVILICMFFNPDTRVSVSIGVVFVVIIWLAYRVHVYRQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ24</accession>
    <name>H4GJ24_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Exopolysaccharide biosynthesis protein, sugar transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122057.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13170"/>
    <dbReference type="EnsemblBacteria" id="EHS87049">
        <property type="protein sequence ID" value="EHS87049"/>
        <property type="gene ID" value="PS3_13170"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.656"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="294" mass="34338" checksum="78DB371808315C35" modified="2012-04-18" version="1">MLISIIAPVYNVAPYFTEFVNSVRHQTIPDFELILIDDGSNDGSSEMCDQFAQEDDRIKVVHQANRGASAARNHGLKLMTGDYVTFVDSDDVLDEDYLQILYDHLISHHADIAVTNYKVFHQANDCFYYYLTDGDLYTRDLTPKEACEYQSNWDSNSSIFIVPFCKLFKAELLEYFQFPEGMIFEDEASIHRLFMKAHKIVLINENHYIYRQGIPSVMTSQMDDKRVEDLIKCFQIKLTDFVMAGYDYIPTRNRFERILLDYQRIFEANQLTDSHGYHLIQEMLLFGSQYHGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ28</accession>
    <name>H4GJ28_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Bacterial cell division membrane protein FtsW</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_12873</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122061.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12873"/>
    <dbReference type="EnsemblBacteria" id="EHS87053">
        <property type="protein sequence ID" value="EHS87053"/>
        <property type="gene ID" value="PS3_12873"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.662"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018365">
        <property type="entry name" value="Cell_cycle_FtsW-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001182">
        <property type="entry name" value="FtsW/RodA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011923">
        <property type="entry name" value="RodA/MrdB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30474">
        <property type="entry name" value="PTHR30474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30474:SF1">
        <property type="entry name" value="PTHR30474:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01098">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00428">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="328"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="392" mass="43646" checksum="22DBA34EEEB6DF0B" modified="2012-04-18" version="1">MRRAIDFLKWRKGATIDWGIVFCVLILAIVGIMSIYVAADNDTSGGAVYRQVISQFVWYVIGAVVVYLVMQMDAEQLWKVAPYVYGASLFLMFAVVIFYSRSYYISTGARSWFAIGPFTFQPSEIAKPAYVLMMGRVITTHNSMYPKHTAKSDWKLIGKMILWLLPLIVSLTLQNDFGTSLVFCAIFAGLVLVSGITWRIIIPSVTIVGAIGGSALWMVTSNFGRSILTMVGFQSYQFDRVDTWLHPEQDTTNQGYQLWQSIKAVGSGGLTGTGFNHSNVYVPVRESDMIFTVIGENFGFIGSIALVAIYMLLIYLMIRVIFETRNEFYAYLSTGVVMMILFHVFENIGMNIGLLPLTGIPLPFVSAGGSSLLGNLIGIGIILSMRYHEERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ64</accession>
    <name>H4GJ64_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_18733</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122097.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18733"/>
    <dbReference type="EnsemblBacteria" id="EHS87089">
        <property type="protein sequence ID" value="EHS87089"/>
        <property type="gene ID" value="PS3_18733"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.711"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="357"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="427"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="455" mass="49388" checksum="DBA0116DA282BCE3" modified="2012-04-18" version="1">MTQTTIKKLGFWSIVLFAINSIIGSGIFLTPGSVVSMVGAKAPLAYLGAAVIAIVLAISFASAAKYVSKSGGAYAYAKASFGDRFGFYMGVVRYFSASVAWGVMAVSVIKSTLSIFGGNPNDFRSVSIGFVILMIIITIVNFLGEEFFTWVNNLSTIGKLTSLVILIIAGVSILLVTGQNNFSQINRLTGNGGLIPSVTTSSFVMAMVAAFYAFTGFESVSSGSEDMIKPEKNLPRAIPLAIIIISIIYIGTISVAMMINPVAIVKTNQVVALAAVFSNRILQTIILLGALVSMFGINVAASFNSPRILEAMANEGQMPHWIAKRNRKGIPIVAYIITVALAILIPMSFLYETTLVILMSAMVRFFEFIVIPIGVVGYYYDKHQQPILNAPKNWWTDVLIPLLGVLLTAFLLFEYDWVGEFSIHGHLNWYAISGMIGGFVVLPIIFALFARWERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ77</accession>
    <name>H4GJ77_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_5367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122110.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5367"/>
    <dbReference type="EnsemblBacteria" id="EHS87032">
        <property type="protein sequence ID" value="EHS87032"/>
        <property type="gene ID" value="PS3_5367"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.731"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016657">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, nitrogenous group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02140">
        <property type="entry name" value="Frm2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033877">
        <property type="entry name" value="Frm2/Hbn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43035">
        <property type="entry name" value="PTHR43035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="201" mass="22332" checksum="92C4694E59E1561E" modified="2012-04-18" version="1">MTNPFIELQSKRHSEYDLNDQVSQSPTQLADLIKTAIKQSPTAFNNQSTRAVITFGKDSDAVWEITLAELKQVMTDDAAIEQTAAKIDTFKAGFGTVLFYTDTDVVKEMQAQFPSYADNFPDWAEQGLGGAQQAVWEALTAEGLGASLQHYNPLIDQEIAERFDIPDSWQLRAEMPFGNVGQKAAVKDSIDDDLRFKTFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ78</accession>
    <name>H4GJ78_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleoside-diphosphate-sugar epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_12430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122111.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12430"/>
    <dbReference type="EnsemblBacteria" id="EHS87033">
        <property type="protein sequence ID" value="EHS87033"/>
        <property type="gene ID" value="PS3_12430"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.733"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="8"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="283" mass="30906" checksum="205ADE2C514A2567" modified="2012-04-18" version="1">MKYAITAATGNFGQTAVNELLKLVDAKDIIVIARNQAKAQTLFPQVEVRQGSYDSVDSMTAALDRVDRVLFISSQPGGEVSRDLQHQNVVQALQNNDVEFVAYTSFPHAQTSISALAQDHRLTENAIIKAGLNHAFLRNNWYLENELGFLQNGANDQVAAYWANNQAGWALEREYAEAAVKVLVSTAPAEIYEFAGPAMTYAQLGEALQAATGNHFEVIQLSQDEYIAALEASGLNHDLATLYASFQLPIEEGSLAENTNDLPTVLGHDLLPITEAIKEVLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ87</accession>
    <name>H4GJ87_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">High-affinity heme uptake system protein IsdE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Iron-regulated surface determinant protein E</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Staphylococcal iron-regulated protein F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_3800</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial solute-binding protein 8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122120.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3800"/>
    <dbReference type="EnsemblBacteria" id="EHS87042">
        <property type="protein sequence ID" value="EHS87042"/>
        <property type="gene ID" value="PS3_3800"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.746"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015886">
        <property type="term" value="P:heme transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019957">
        <property type="entry name" value="ABC_transptr_haem-bd_IsdE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03659">
        <property type="entry name" value="IsdE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="7">
        <location>
            <begin position="37"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="291" mass="32051" checksum="BC4600CBAF609995" modified="2012-04-18" version="1">MKRKVIIGSLLAFLVIIGGIAWGARVFVQKQQEQSQRIVATSAAITEIFAKLDLDLVGVPKTQAKLPARYQNVAKIGSPMAPSVEKIASLNPTTVYAVSTLKDQYNSAFKKQAVNVVYLKLDSVNQLKGTLSSLGKQYHRTRQAHAANQEIDSAITTVKKRVHGRKPRVLILMGMPGAGYMIMTEKTYVGDLVKLAGGENVYAANGQSFISPNNESLAQQKPDVILRLAHALPDQTIPQFNEEFKNNPVWAQMPAVKNHRVYDLKQPTFNASANINAAKALKQVSQWLYPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ93</accession>
    <name>H4GJ93_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dinucleotide-binding enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_17748</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122126.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17748"/>
    <dbReference type="EnsemblBacteria" id="EHS87048">
        <property type="protein sequence ID" value="EHS87048"/>
        <property type="gene ID" value="PS3_17748"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.758"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="189" mass="19953" checksum="6863AD704F150641" modified="2012-04-18" version="1">MKVSVIGKGNMGQAIAKNFEAAGQSVEFIGHGEAQPTFGDLVVFAVPFAAEKDLANQYQAQLAGKIVVEIANPLNFDNWDELVVPSDSSAAEILQTVLPQSKVLKAFNTTFAGTLNSGQVGDNQTTVLVAGDDEDAKETFASALSGSPLTVMDAGKLKRAREMEALGFLQMTLAAQDKISWNGGFGINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJB5</accession>
    <name>H4GJB5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_13009</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000032">
        <property type="protein sequence ID" value="EHS87005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13009"/>
    <dbReference type="EnsemblBacteria" id="EHS87005">
        <property type="protein sequence ID" value="EHS87005"/>
        <property type="gene ID" value="PS3_13009"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.808"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="6">
        <location>
            <begin position="101"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="101"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3">
        <location>
            <position position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="204" mass="23273" checksum="449D22B2D08429DF" modified="2012-04-18" version="1">MYDISTVSNGQQALAYIEENSVDMLISDVMMPIMDGFTLVKTLRQDGYDFPIIMLTAKETIRDKTIGFTSGVDDYITKPVNLDELVLRIAALMRRAKIQSSRVIKIGETSINEATLLVQKEKITVELANKEFALLFKLLSYPNQVFTTSQLFDEIWGLDSDSNEDTVKTHISRLRKKLSDFKDDFEIQTVRGLGYRGILHHEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJC4</accession>
    <name>H4GJC4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Allophanate hydrolase subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_16007</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000033">
        <property type="protein sequence ID" value="EHS86993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122157.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000033.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16007"/>
    <dbReference type="EnsemblBacteria" id="EHS86993">
        <property type="protein sequence ID" value="EHS86993"/>
        <property type="gene ID" value="PS3_16007"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.825"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003833">
        <property type="entry name" value="CT_C_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010016">
        <property type="entry name" value="KipI_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34698">
        <property type="entry name" value="PTHR34698"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02682">
        <property type="entry name" value="CT_C_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00796">
        <property type="entry name" value="AHS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00370">
        <property type="entry name" value="TIGR00370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AHS1" evidence="4">
        <location>
            <begin position="6"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="239" mass="26654" checksum="35246B5885952308" modified="2012-04-18" version="1">MDKPNYQLIPLGDQAIMVQMANQIDPQENQRLQAIARLISGPEILGVVPAYSTLTVNFDAQQTTMARMMDRLDEVIQQSLQMELPPAKTWLIPVSYGGEFGPDLGDVADFAQTSPEEVIKRHTDTDYLIYFLGFLPGFAYMGSVDQQIAMPRLATPRLSIPAGSIGIAGQQTGFYPVASPGGWRLIGQTPIRLYDPQSPQSFYQAGDYVRFVPVNQREFKQIQAAVERDQYQVEVIDHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJD3</accession>
    <name>H4GJD3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hemolysin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_11046</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TlyA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000035">
        <property type="protein sequence ID" value="EHS86882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122166.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000035.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11046"/>
    <dbReference type="EnsemblBacteria" id="EHS86882">
        <property type="protein sequence ID" value="EHS86882"/>
        <property type="gene ID" value="PS3_11046"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.841"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004538">
        <property type="entry name" value="Haemolysin_A/TlyA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32319:SF0">
        <property type="entry name" value="PTHR32319:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005578">
        <property type="entry name" value="TlyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00478">
        <property type="entry name" value="tly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FtsJ" evidence="2">
        <location>
            <begin position="61"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="273" mass="30161" checksum="1BAB0391A30B42F2" modified="2012-04-18" version="1">MGKERVDVLLVCQGLFDSREQAKRAVMAGEVLGKNSERLDKPGQKIDETTTFRLKGKKMPYVSRGGLKLAKALQVFEISVKDKIVLDIGSSTGGFTDVMLQNGAKLSYALDVGTNQLVWQLREDPRVVVMEQTNFRYSKLEDFTAGQPKFSSIDVSFISLQLIFPPLAEILPVDGQVVALIKPQFEAGKDKVGKHGIVKDPKVHRQVIQQVIGYANEAHFDVLNLDFSPIKGGQGNIEFLVHLKLSDRPGVVSNAVNIDQVLAMAKQELNTQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJG2</accession>
    <name>H4GJG2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Galactofuranosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_13024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000038">
        <property type="protein sequence ID" value="EHS86716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122195.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000038.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13024"/>
    <dbReference type="EnsemblBacteria" id="EHS86716">
        <property type="protein sequence ID" value="EHS86716"/>
        <property type="gene ID" value="PS3_13024"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.914"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="353" mass="40622" checksum="9E0CE31E20E8DAE7" modified="2012-04-18" version="1">MQNYIISLKDPSGNVGGPKANMDNIKFLKEQMGFQEFWLDYGWKDGFWWHHNLWDWINTRLHKYQLSHSILPNFFKEHPDIDNVVIQYPLYSNKLVKQIADSVHQNSHAKLYFIIHDAEMIRLYADEPEKGQAEVDSFNLADGIIGHNAKMNDFLRQNGVKVPLVDLGIFDYDNPQPIQPYHGYDKSVCYAGNLIDAVFLEKVHPTNRFDLFGPNPAEQYNDGLNYRGQFSPTELPAHMDENFGLVWHGTSVDTCDGTFGRYLKWNNPHKTSLYLSSGLPVIIWDQAALADFVTENGVGITISSLNELNEKLDALTEDEYRQIHQNVEKIANQMRSGYYITHAMQKMLATSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJH4</accession>
    <name>H4GJH4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sucrose phosphorylase</fullName>
            <ecNumber evidence="4">2.4.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Sucrose glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_8193</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>phosphate + sucrose = alpha-D-glucose 1-phosphate + D-fructose</text>
            <dbReference type="Rhea" id="RHEA:24048"/>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 13 family. Sucrose phosphorylase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.7" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000039">
        <property type="protein sequence ID" value="EHS86617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122207.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000039.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8193"/>
    <dbReference type="EnsemblBacteria" id="EHS86617">
        <property type="protein sequence ID" value="EHS86617"/>
        <property type="gene ID" value="PS3_8193"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.927"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009018">
        <property type="term" value="F:sucrose phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044262">
        <property type="term" value="P:cellular carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11355">
        <property type="entry name" value="AmyAc_Sucrose_phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016377">
        <property type="entry name" value="Sucrose_GGa_phosphorylase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022527">
        <property type="entry name" value="Sucrose_phospho"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003059">
        <property type="entry name" value="Sucrose_phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03852">
        <property type="entry name" value="sucrose_gtfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aamy" evidence="7">
        <location>
            <begin position="6"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>sucrose</name>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>sucrose</name>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="194"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>sucrose</name>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>sucrose</name>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="294"/>
            <end position="295"/>
        </location>
        <ligand>
            <name>sucrose</name>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="338"/>
            <end position="341"/>
        </location>
        <ligand>
            <name>sucrose</name>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>sucrose</name>
            <dbReference type="ChEBI" id="CHEBI:17992"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR003059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR003059-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR003059-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="485" mass="55443" checksum="438758B06E2A03E3" modified="2012-04-18" version="1">MPIENKTMLITYSDSMGKNIKETHEILKEYIGDAIGGVHLLPFFPSTGDRGFAPYDYTVVDSAFGNWDDVEAMGEDYYLMFDFMINHISKKSVQYQDFKKNHDDSKYNDFFIRWEKFWEAAGPNRPTQEDVDKIYKRKDKAPMQEITFDDGTTEHLWNTFGEEQIDINVKSKVANEFFKTTLIDMVKHGADLIRLDAFAYAIKKVDSDDFFVEPEIWDLLNEVRDILAPYQAEILPEIHEHYSIPAKISDHGYFVYDFALPMTTLYTLYSGKTNRLAKWLKMSPMKQFTTLDTHDGIGVVDARDILTDDEIDYASEELYKVGANVKKKYSSAAYNNLDIYQINSTYYSALGDNDKAYLLSRAFQVFAPGIPMVYYVGLLAGSNDLELLESTKEGRNINRHYYNKDEIAQEVQRPVVANLLKLLAWRNEFAAFDLDGSIDVQTPSETTITITRKDASGDNVAVLTADAANKTFTITANGEVVMEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJK7</accession>
    <name>H4GJK7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein of hypothetical function DUF985</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_5142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122240.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5142"/>
    <dbReference type="EnsemblBacteria" id="EHS86517">
        <property type="protein sequence ID" value="EHS86517"/>
        <property type="gene ID" value="PS3_5142"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.981"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06121">
        <property type="entry name" value="cupin_YML079wp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009327">
        <property type="entry name" value="Cupin_DUF985"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039935">
        <property type="entry name" value="YML079W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33387">
        <property type="entry name" value="PTHR33387"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06172">
        <property type="entry name" value="Cupin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_5" evidence="1">
        <location>
            <begin position="5"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="166" mass="18911" checksum="4209969864F11262" modified="2012-04-18" version="1">MTTKQEYIEQLRLEAHPEGGWYRQVYHSAKSVYDQTSLASRYQYTSIYFILDGSSPSHLHRLLHDEIWYYHDGAPIVVHCFSPNGDYTAVKLGRNLAAGEQLQFRVPAGTIFGSEVTDPTSFGFVSCAVAPGFDYHDFELYTQAQLLPIFPEQANIIKRLAYQNLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJN6</accession>
    <name>H4GJN6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome-binding ATPase YchF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ychF</name>
        <name evidence="7" type="ORF">PS3_4928</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000045">
        <property type="protein sequence ID" value="EHS86237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122269.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000045.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4928"/>
    <dbReference type="EnsemblBacteria" id="EHS86237">
        <property type="protein sequence ID" value="EHS86237"/>
        <property type="gene ID" value="PS3_4928"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1024"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00944">
        <property type="entry name" value="YchF_OLA1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006641">
        <property type="entry name" value="CHP00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00092">
        <property type="entry name" value="TIGR00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="5">
        <location>
            <begin position="3"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="6">
        <location>
            <begin position="280"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS86237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="365" mass="39932" checksum="2AFCBC7A8104DC6B" modified="2012-04-18" version="1">MALTAGIVGLPNVGKSTLFNAITKAGAEMANYPFATIDPNVGMVEVPDDRLARIDELIPAKKIVLTTFEFTDIAGIVKGASKGEGLGNKFLENIRQTDAIVHVVRAFDDDDITHVTGKVDPIDDIDTINLELIMADLDSVNNRLSKVQRAAKGRDKDALAELAVLEKIKPVLEAGQSARTIDFSDEEQPIVKGLFLLTTKPVLYVANVAEDDMADPESNPYIAQIREHVQNEGEVIGIAAAAEEEIAEMDDDEKADFLEMEGVTEPGLNRLIRAAYKLLGLETFFTAGGKETRAWTYRKGTKAPQAAGIIHSDFERGFIRAEVMSFEDLDQYGSPAAVKEAGRLRLEGKDYVMQDGDIVEFRFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJP2</accession>
    <name>H4GJP2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_4576</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000045">
        <property type="protein sequence ID" value="EHS86243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122275.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000045.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4576"/>
    <dbReference type="EnsemblBacteria" id="EHS86243">
        <property type="protein sequence ID" value="EHS86243"/>
        <property type="gene ID" value="PS3_4576"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1031"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="335"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="398"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="426"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="AA_permease" evidence="6">
        <location>
            <begin position="16"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS86243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="450" mass="49973" checksum="ABC63338F1E714F5" modified="2012-04-18" version="1">MEEQEQKLSRSLKSRHITMIAIGGAIGTGLFLGSGSAIRTAGPSIILTYLIVGIFCFFMMRALGELLLSDTSKHSFIDLVRDYLGEQFEFIAGWTYWSCWLSLAMADLTATGIYLQYWFPSLPQWVGPLVIIVLLMLINMINVGWFGEFESWFSAIKVIAIVALIVVGIVMIILHTKVSTGYTSLTNLYSHGGFFPFGFGGFLTSFQMVVFAFVGIEMVGLTAGETRNPQKDIPKAINSLPIRIGLFYIGAMTAIMAIYPWNQIKTTSSPFVQVFAGIGVAGAAGILNFVVLTSAMSATNSAIFSTSRSLYALAENQQAPKQYAKLSNKAVPNRALQVSSLILFIVVILNYIMPSGIFNIISGVSTINFVFVWLIILWTHLAYRRVHPEGVAGFSMPWYPYTSWATIIFFIFVLIILLFIPSTRPSLIISIIFFIVLFIGYYALQRRKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJQ0</accession>
    <name>H4GJQ0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Universal stress protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_14273</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000046">
        <property type="protein sequence ID" value="EHS86232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122283.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000046.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14273"/>
    <dbReference type="EnsemblBacteria" id="EHS86232">
        <property type="protein sequence ID" value="EHS86232"/>
        <property type="gene ID" value="PS3_14273"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1042"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006276">
        <property type="entry name" value="UspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="3">
        <location>
            <begin position="4"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR006276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="166" mass="18015" checksum="A96E7A2EC0692EF0" modified="2012-04-18" version="1">MAEYKNILVGIDGSKQSLAAFDKAVLLAKQNQATLHLLSVVNGERYPTADTIGYGFIDRDVYQESIQKMESTLADYKQRALDAGVPEVLTKVVIGNVKLELSSGYAEDHDIDLLVVGATGLNVIGKMLVGSTANYVIGHTAYDVVVVKTDINNDPLKIDRTAYPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJR7</accession>
    <name>H4GJR7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative sugar-phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_13235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000047">
        <property type="protein sequence ID" value="EHS86217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122300.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000047.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13235"/>
    <dbReference type="EnsemblBacteria" id="EHS86217">
        <property type="protein sequence ID" value="EHS86217"/>
        <property type="gene ID" value="PS3_13235"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1075"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000150">
        <property type="entry name" value="Cof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00099">
        <property type="entry name" value="Cof-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="269" mass="29997" checksum="A3B2088EDE17BF0D" modified="2012-04-18" version="1">MTLPFAAVAFDMDGTFLTDDKTFDHQLFNEIASQLRAQDVQLIVASGNSLECLLNYFTPEEQAELTFLSENGAHVVNQAGDLLISQTLDPAIAQAVISYLVNDLHCYPSLAGTKYGYLPAQESPANLERMKFYFPKYQVIDDYDHLPEDRYYQMSFLIDEQTESRINDLIQRFGDQVSITPSGNGSVDITVPGIDKAWALNHLLDQWQLRSDQLAAFGDGDNDLGMLKLAKYSYAMQNGNANVKAVANYQTKTDNNHNGVLQSLADYLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJU7</accession>
    <name>H4GJU7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10 11">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="7 10 11">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rpe</name>
        <name evidence="15" type="ORF">PS3_7961</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10 11">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10 13">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 10 11">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="7 10 11"/>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122330.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7961"/>
    <dbReference type="EnsemblBacteria" id="EHS86134">
        <property type="protein sequence ID" value="EHS86134"/>
        <property type="gene ID" value="PS3_7961"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1125"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="PTHR11749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001461">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01163">
        <property type="entry name" value="rpe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="13" id="KW-0170">Cobalt</keyword>
    <keyword evidence="9 10 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="10 14">
        <location>
            <begin position="141"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="174"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10 14">
        <location>
            <begin position="196"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10 12">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10 12">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 14">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13 14">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EHS86134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="217" mass="23273" checksum="B28484C83546F7B2" modified="2012-04-18" version="1">MIKVAPSILSADYVNLQKDIELVEQGGAEYLHIDVMDGSFVPSISYGPGFVKAIRPISKMVLDVHLMVQNPEHIIPDFVEAGADIIGVQVEATQHIHRALQIIKNGGVKAEVVINPGTPVEMIKPVLHMVDQVLVMTVNPGFGGQKFLPETVAKIAELNALKQENGYNFDIEIDGGVNNETVVPCYEAGATVAVAGSYVYGADDPVAKINAIKDATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJZ0</accession>
    <name>H4GJZ0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cadmium efflux ATPase CadA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_6591</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS86007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6591"/>
    <dbReference type="EnsemblBacteria" id="EHS86007">
        <property type="protein sequence ID" value="EHS86007"/>
        <property type="gene ID" value="PS3_6591"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1196"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="508"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS86007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="558" mass="59905" checksum="0F90401D580A81CA" modified="2012-04-18" version="1">MLIEMIKKMIHGDFGVDILAVTAIVATLAVGDYWASMIILLMLIGGDSLEDYANRKANSELKSLLDNSPEQAHRLNGDQVEDIKADSVKVDDVLLIRPGELVPVDGRVLEGDSNLDESSLTGESVPVEKTVGDQVLSGSVNTEGMLKVVAERTAANSQYQQLIHLIQQAENDPGRFVRLADRYAIPFTIVAYVLAGIAWWISKDPVRFAQVLVVASPCPLILAAPVAFVSGMGRSSRNGIVVKTGDSIEKLATAKTMAFDKTGTLTQGNLTVDQVKPADHFTSDEVLQLAASVEQNSGHVIAQSITNYALDHQIKLLTTNEVKEVTGNGLQAMVNGHLVKVGKLIFVTDNQAIKPLNQTASYVSVDGQYAGCITLEDQLRPETIRTISELHQLKINNQMMLTGDQEETAKRIGQEIGFDQVAANLLPADKISRIKALTPDQRPAVMVGDGVNDAPVLKTADVGIAMGARGSTAASESADIVIMTDNLEKVASAVLIARDTLKVAKQSVLIGIFICIGLMLICMTGWIPAIIGALLQEVIDTVCILWSLKARTGQKLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJZ6</accession>
    <name>H4GJZ6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome maturation factor RimM</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rimM</name>
        <name evidence="7" type="ORF">PS3_10006</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Binds ribosomal protein S19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">The PRC barrel domain binds ribosomal protein S19.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RimM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS86013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122379.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10006"/>
    <dbReference type="EnsemblBacteria" id="EHS86013">
        <property type="protein sequence ID" value="EHS86013"/>
        <property type="gene ID" value="PS3_10006"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1209"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00014">
        <property type="entry name" value="Ribosome_mat_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011961">
        <property type="entry name" value="16S_RimM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002676">
        <property type="entry name" value="RimM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036976">
        <property type="entry name" value="RimM_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01782">
        <property type="entry name" value="RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02273">
        <property type="entry name" value="16S_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="RimM" evidence="5">
        <location>
            <begin position="6"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="PRC" evidence="6">
        <location>
            <begin position="95"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS86013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="169" mass="18888" checksum="9D61B89ABC5974CE" modified="2012-04-18" version="1">MTLYKVGKIVNTHGIRGEVKVITTTDFVEERFQVGNQLWIEQAGQAPIAVTIKQARPHKGTMLVKFAEFDNINEVEHFRNAQLSVTEADQAELEEGQYYYRQIIGLKVQTIDGRSLGTIKEILAPGANDVWVVQREGQSDLLLPVIDDVIKKVDLDAGLVTVELLEGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK07</accession>
    <name>H4GK07_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dTDP-4-dehydrorhamnose 3,5-epimerase</fullName>
            <ecNumber evidence="3">5.1.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Thymidine diphospho-4-keto-rhamnose 3,5-epimerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_19593</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose</text>
            <dbReference type="Rhea" id="RHEA:16969"/>
            <dbReference type="ChEBI" id="CHEBI:57649"/>
            <dbReference type="ChEBI" id="CHEBI:62830"/>
            <dbReference type="EC" id="5.1.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dTDP-4-dehydrorhamnose 3,5-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.13" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000053">
        <property type="protein sequence ID" value="EHS85879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122390.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000053.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19593"/>
    <dbReference type="EnsemblBacteria" id="EHS85879">
        <property type="protein sequence ID" value="EHS85879"/>
        <property type="gene ID" value="PS3_19593"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1229"/>
    <dbReference type="UniPathway" id="UPA00124"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008830">
        <property type="term" value="F:dTDP-4-dehydrorhamnose 3,5-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019305">
        <property type="term" value="P:dTDP-rhamnose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000888">
        <property type="entry name" value="dTDP_sugar_isom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21047">
        <property type="entry name" value="PTHR21047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00908">
        <property type="entry name" value="dTDP_sugar_isom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01221">
        <property type="entry name" value="rmlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="site" description="Participates in a stacking interaction with the thymidine ring of dTDP-4-oxo-6-deoxyglucose" evidence="2">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="193" mass="21781" checksum="EC66A865BF13B707" modified="2012-04-18" version="1">MGKLVVKTTKLQDVKLITPAVFGDHRGFFTETYSDRDFKDAGIDFEFIQDNQSLSSQAGVLRGLHFQRGRAAQTKLIRVVTGAVLDVIVDLRKGSPTYKQWEGYILSASNHRQLLVPRGFAHGFLTLTDNVNFVYKCDNYYDDEADGGISFKTPELAIDWPIDFDKAITSEKDSKQPTLTEFEVDNPFVYGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK14</accession>
    <name>H4GK14_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_5151</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the thioredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000054">
        <property type="protein sequence ID" value="EHS85866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122397.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000054.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5151"/>
    <dbReference type="EnsemblBacteria" id="EHS85866">
        <property type="protein sequence ID" value="EHS85866"/>
        <property type="gene ID" value="PS3_5151"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1240"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="5">
        <location>
            <begin position="28"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="105" mass="11873" checksum="0D8BB97BE15FB4F7" modified="2012-04-18" version="1">MIHETTDTTFQNDTQQGVQLIDFWAQWCGPCRMQTPVVEELAETMGNQVSFHKLNVDCNQATAKECQIMSIPTLVIKKDGVVVDKISGYHSKEQLAQVLQAYLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK23</accession>
    <name>H4GK23_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="3 13">6.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 13">AIR synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 13">AIRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10 13">Phosphoribosyl-aminoimidazole synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">purM</name>
        <name evidence="16" type="ORF">PS3_1765</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the AIR synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1" evidence="3 13"/>
    <dbReference type="EMBL" id="AICN01000055">
        <property type="protein sequence ID" value="EHS85839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122406.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000055.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_1765"/>
    <dbReference type="EnsemblBacteria" id="EHS85839">
        <property type="protein sequence ID" value="EHS85839"/>
        <property type="gene ID" value="PS3_1765"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1255"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="PTHR10520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 13" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="14">
        <location>
            <begin position="57"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="15">
        <location>
            <begin position="173"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EHS85839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="345" mass="37073" checksum="8ADB3D9D9EF7627E" modified="2012-04-18" version="1">MNRYQEAGVDVNAGYELVDRIKQDVKSTNRPGVMGGIGAFGGLFDLGSLKVQHPILVSGTDGVGTKLLIAQEMDKHDTIGIDVVAMCVNDVLAQGAEPLYFLDYIATGHNDPKKMAAIVSGVAEGCRQAGAALIGGETAEMPDMYSFEEYDLAGTVTGVVDKDDLLTSDNPVAGDILIGLASSGIHSNGFSLVRQILFKDHQVALSDQPATLAGQSVGEAILQPTRIYVPFVLPLIKQHLITGVSHITGGGLVENLPRMFGDDLQAVVDSSTWPQLPVFNYLRELGELPLHDCFETFNMGIGMVLAVHPDQVTMVQNQLRTLDEESYIIGELKARPEDEAKIVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK30</accession>
    <name>H4GK30_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">N5-carboxyaminoimidazole ribonucleotide synthase</fullName>
            <shortName evidence="5 7">N5-CAIR synthase</shortName>
            <ecNumber evidence="5 7">6.3.4.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 7">5-(carboxyamino)imidazole ribonucleotide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">purK</name>
        <name evidence="9" type="ORF">PS3_6541</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="function">
        <text evidence="7">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 7">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ATP + hydrogencarbonate = 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + ADP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19317"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5 7">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 7">Belongs to the PurK/PurT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.18" evidence="5 7"/>
    <dbReference type="EMBL" id="AICN01000055">
        <property type="protein sequence ID" value="EHS85846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6541"/>
    <dbReference type="EnsemblBacteria" id="EHS85846">
        <property type="protein sequence ID" value="EHS85846"/>
        <property type="gene ID" value="PS3_6541"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1262"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00942"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034028">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004638">
        <property type="term" value="F:phosphoribosylaminoimidazole carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01928">
        <property type="entry name" value="PurK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005875">
        <property type="entry name" value="PurK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040686">
        <property type="entry name" value="PurK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17769">
        <property type="entry name" value="PurK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01161">
        <property type="entry name" value="purK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 5 7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="8">
        <location>
            <begin position="108"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="149"/>
            <end position="155"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="179"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="264"/>
            <end position="265"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS85846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="374" mass="41104" checksum="4D2ADE3693A474F0" modified="2012-04-18" version="1">MLLPGSTIGIIGGGQLGQMMALDAKENGMQVVILDPTPACPAGQVADHQIVADYDDFTAIEELAHRSNVLTYEFENVDLAALEKVADQVAIPQGTELLRITKDRLREKRFLRVAKVQTAPFMEVESEADLEKALASIGYPAVLKTAQGGYDGHGQAVLKSAADLPAAQAVLAAGSCILEGWVDFDLECSVMVARNTDGEVVTFPVAENIHHNEILHKTIVPARISPQLQDRARQMAVQIAEQLDLKGILGVEMFVKRDGDIYINELAPRPHNSGHYSIEACDFSQFAMHNRAILNWPLPEVTLLKPAIMVNVLGQHVKPVQALISQKPNWHFHDYGKAESRENRKMGHVTILTDDLETTLAEIADTKVWQAEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK60</accession>
    <name>H4GK60_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C</fullName>
            <shortName evidence="6">Asp/Glu-ADT subunit C</shortName>
            <ecNumber evidence="6">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">gatC</name>
        <name evidence="7" type="ORF">PS3_19786</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 6">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4 6">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the GatC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000059">
        <property type="protein sequence ID" value="EHS85694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122443.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000059.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19786"/>
    <dbReference type="EnsemblBacteria" id="EHS85694">
        <property type="protein sequence ID" value="EHS85694"/>
        <property type="gene ID" value="PS3_19786"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1305"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00122">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="Asp/Glu-ADT_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004">
        <property type="entry name" value="PTHR15004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="Glu-tRNAGln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="SSF141000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00135">
        <property type="entry name" value="gatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="105" mass="11721" checksum="24A4F6B794AE64B1" modified="2012-04-18" version="1">MAENLDREQVQHVAELAKLSFTDAELDHFVPQLEEIIDLFNQLAEVDTEQVKPMTTATDEVNVLREDEAVKSSEAMRQALLANAPETEDGLIRVPAILDESEDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK64</accession>
    <name>H4GK64_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-acetylmuramidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_3346</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 73 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000059">
        <property type="protein sequence ID" value="EHS85698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3346"/>
    <dbReference type="EnsemblBacteria" id="EHS85698">
        <property type="protein sequence ID" value="EHS85698"/>
        <property type="gene ID" value="PS3_3346"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1310"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002901">
        <property type="entry name" value="MGlyc_endo_b_GlcNAc-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01832">
        <property type="entry name" value="Glucosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00047">
        <property type="entry name" value="LYZ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="LYZ2" evidence="3">
        <location>
            <begin position="35"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="194" mass="22259" checksum="71E2A171CA36AE65" modified="2012-04-18" version="1">MKWTIIILVMLVALVGAYFGRHVYMRYRQQYEQQQTIEKQAKEKRDFIKKIAPEAQKMQTKYHVMSSITIAQAILESNWGKSSLAKDYHNLFGIKGSGANSKVMSTKEYTNGQWVTISGRFKVYSSWNASIADHTKLMLNGTYYNSNNYSAVVNATNYKDAAQALQDADYATDPDYAKKLINIIQTYDLDQYDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK65</accession>
    <name>H4GK65_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_17325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000061">
        <property type="protein sequence ID" value="EHS85626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17325"/>
    <dbReference type="EnsemblBacteria" id="EHS85626">
        <property type="protein sequence ID" value="EHS85626"/>
        <property type="gene ID" value="PS3_17325"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1311"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="102" mass="12106" checksum="8AC284E44505734E" modified="2012-04-18" version="1">MNLVTKSFRQEQRIMEEKTFISWLGKIASVIAILIYVSYVFNIIQNFHGNPVSWEQPFVAGINCSLWSIYAYFKQHRDWPVFWANFPGVFFSFATVITCFWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK72</accession>
    <name>H4GK72_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_3764</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000061">
        <property type="protein sequence ID" value="EHS85633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122455.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000061.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3764"/>
    <dbReference type="EnsemblBacteria" id="EHS85633">
        <property type="protein sequence ID" value="EHS85633"/>
        <property type="gene ID" value="PS3_3764"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1323"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221">
        <property type="entry name" value="PTHR33221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02082">
        <property type="entry name" value="Rrf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51197">
        <property type="entry name" value="HTH_RRF2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS85633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="153" mass="17280" checksum="02B485C30AFDDD66" modified="2012-04-18" version="1">MRISTRFSDAIHLICFLDIYQNQKLTSDFIASSIQTSSVMVRQIMIQLRKAGLLKTTKGTANPQITRPLDEISLYDVYLAIEEASGPHPLFEIDYDTNPNCIVGGNIQGVLESYYEDAKQAAMAKLQQRTIADVVNQIKISQSKKELKQRQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK88</accession>
    <name>H4GK88_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Citrate lyase alpha chain</fullName>
            <shortName evidence="1">Citrase alpha chain</shortName>
            <ecNumber evidence="1">2.8.3.10</ecNumber>
            <ecNumber evidence="1">4.1.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Citrate (pro-3S)-lyase alpha chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Citrate CoA-transferase subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_9101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + citrate = (3S)-citryl-CoA + acetate</text>
            <dbReference type="Rhea" id="RHEA:19405"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57321"/>
            <dbReference type="EC" id="2.8.3.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>citrate = acetate + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:10760"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="EC" id="4.1.3.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.3.10" evidence="1"/>
    <dbReference type="EC" id="4.1.3.6" evidence="1"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122471.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9101"/>
    <dbReference type="EnsemblBacteria" id="EHS85540">
        <property type="protein sequence ID" value="EHS85540"/>
        <property type="gene ID" value="PS3_9101"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1347"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009346">
        <property type="term" value="C:ATP-independent citrate lyase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008815">
        <property type="term" value="F:citrate (pro-3S)-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008814">
        <property type="term" value="F:citrate CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006472">
        <property type="entry name" value="Citrate_lyase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40596">
        <property type="entry name" value="PTHR40596"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04223">
        <property type="entry name" value="CitF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009451">
        <property type="entry name" value="Citrt_lyas_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01584">
        <property type="entry name" value="citF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="512" mass="55207" checksum="F4E72E5552548B89" modified="2012-04-18" version="1">MKNKVGREISDEVLAQTDYQPFETTEIGHPVVTYTDHQVRVTKGDDKLVDSLTQVIKDTLKDGMTISFHHHFRNGDYVFNMVMDQIIKLGYQNLTLAPSSLTGVMNDKVIEAIKAGTVTNITSSGMRGSLGEAISHGLMKNPVIFRSHGNRARSIENGNIKIDVAFLGVPNADRMGNANGMEGESTLGSLGYALVDAKYADKVVLLTDTIADYPNTPASIQQTQVDYVVKVDKVGDPDRIGSGATRFTKNPKELKIAKMVNDVIVNSPYFKQDFSFQTGTGGASLAVARFLRQSMLDHHITASFTCGGTTSPIVELLEEGLVKKVMDVQDFDKGAAASMKKNPGQTEIDGSWYADPDNKGAMVDQLDIAILSALEIDTDFNVNVLTGSNGEIRGAVGGHQDAGTAKMTIISAPLTRGRIATIVPAVNSVTTPGSSIDVVVTEVGVAVNPARQDLIEAFKAHPSIPLVSIEELQARAAKIVGQPKPVEYTDRTVALVEYRDGTLIDEIKQIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK98</accession>
    <name>H4GK98_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Metabolite transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_9704</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122481.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9704"/>
    <dbReference type="EnsemblBacteria" id="EHS85550">
        <property type="protein sequence ID" value="EHS85550"/>
        <property type="gene ID" value="PS3_9704"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1358"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="340"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="376"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="25"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS85550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="435" mass="47718" checksum="67473CD4DA576153" modified="2012-04-18" version="1">MDLSAEQITITRLNNAKESPTFYKVFTLVGLGLMIDAADVYMASSTNTAMITSKFATEAQGSTFLSAGFLGLFIGSIIAGYFGDYFGRRKAYQANLFIFGAATLLGGFAPNIAILSILRFIATIGLGAEIVTAFATINEFAPTKHRGRWSGAISVIGNIGSPLGFLLCMLIIPDFGWRPLFFIIGIAALILWAVRRHNFPESPRWLISKGRTEEAEKIIQAMSINGYYDPIEEHENKGQQSTTIKVGLKRAFIVGIIVAMATTVCQYTFTSWVPTILVKEGVDIFHSLMYSTVMMIGAPLGALVGASVVDRAGRKWTIGIGFLVIAILGIIYTYQKSDVAVMMIGFCLTMAMYAMNASILSVYVPELFPTKYRFRGEGYAAGAGKLMNVLMPYFIVWLMTNFATSYIFYSIAILAIIASIVTFAFGPETKQRYIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKC4</accession>
    <name>H4GKC4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Galactose operon repressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_19213</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122507.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19213"/>
    <dbReference type="EnsemblBacteria" id="EHS85576">
        <property type="protein sequence ID" value="EHS85576"/>
        <property type="gene ID" value="PS3_19213"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1395"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="2"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="334" mass="36834" checksum="1DAD73B4BBDFE0A0" modified="2012-04-18" version="1">MATIKDIAKKAQVSPTTVSRVLSYDPTLSVNNETRKKVFAAAEALNYTKYQTKYQYRHNGEQLFVVMWCSAQQEINDVYYFSIRQGIESEAKREGYQVTTIYADESWEPLANGAGVIVVGSNQYGASQLAFLKKLDLPVAFADGDLLGQGFASVTIDTTDAVKHVLDHFAKHGQKEVGILVGDLADQYDKDNLIDFRFQDFKALMTQRGLYRSDHVFVGAFTPDSGYAAIKKAIDEGIKLPSALFVANDAMAIGAVKAFQEAGIQIPDQLSLISFNDTSVAQYAFPTLSTVSVNTNQLGQLAVKVIADLQENGQREPYKITLQTKLILRDSSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKE0</accession>
    <name>H4GKE0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">3-isopropylmalate dehydratase small subunit</fullName>
            <ecNumber evidence="10">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Alpha-IPM isomerase</fullName>
            <shortName evidence="10">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">leuD</name>
        <name evidence="12" type="ORF">PS3_5338</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 10">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the LeuD family. LeuD type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="10"/>
    <dbReference type="EMBL" id="AICN01000063">
        <property type="protein sequence ID" value="EHS85504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122523.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000063.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5338"/>
    <dbReference type="EnsemblBacteria" id="EHS85504">
        <property type="protein sequence ID" value="EHS85504"/>
        <property type="gene ID" value="PS3_5338"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1433"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009316">
        <property type="term" value="C:3-isopropylmalate dehydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01031">
        <property type="entry name" value="LeuD_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004431">
        <property type="entry name" value="3-IsopropMal_deHydase_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00171">
        <property type="entry name" value="leuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase_C" evidence="11">
        <location>
            <begin position="5"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS85504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="194" mass="22034" checksum="787D66C56616B34C" modified="2012-04-18" version="1">MEPIVVHTGTTIPIMNDDIDTDQIIPKQFLKNLLKTGYENALFYNWRYLPDGRPDPKFILNAPERQAATILITGSNFGSGSSREHAAWALKDWGIRVVIAGSFSDIFYMNCTENGVLPIKLPQTARDQLAQLSSDQAIVVDLPNQVVKNGEDTYPFEIDQTWKEKFISGDDDIDITMKHIQEIEAYEARIPQYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKE2</accession>
    <name>H4GKE2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_2619</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000064">
        <property type="protein sequence ID" value="EHS85434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2619"/>
    <dbReference type="EnsemblBacteria" id="EHS85434">
        <property type="protein sequence ID" value="EHS85434"/>
        <property type="gene ID" value="PS3_2619"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1437"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="134" mass="15732" checksum="A5446747F4146F2F" modified="2012-04-18" version="1">MATFVERKSRLTIVKRLHGRDSQSMTQAVLELASQLQGKLKTLTVDHGKEFANYQTIERRTGTQVYFAHAYSPHERGSNENRNRVLRHFIPKGQAIEELSDHKLIQINWYLNSRPLKCLNWHTPIEIFLLNLRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKE4</accession>
    <name>H4GKE4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative RelB-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_8817</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RelB/DinJ antitoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000064">
        <property type="protein sequence ID" value="EHS85436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122527.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000064.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8817"/>
    <dbReference type="EnsemblBacteria" id="EHS85436">
        <property type="protein sequence ID" value="EHS85436"/>
        <property type="gene ID" value="PS3_8817"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1445"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007337">
        <property type="entry name" value="RelB/DinJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04221">
        <property type="entry name" value="RelB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02384">
        <property type="entry name" value="RelB_DinJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="93" mass="10329" checksum="2F8F02A95B3EDF40" modified="2012-04-18" version="1">MAVKEKKRVQVKIDKDLADNTETVLNELGLNPTTAINMFYKRIVAEAALPFKPALSEAERANLGLLKATKETPVTEFKDAKEVADWLNDPDEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKE7</accession>
    <name>H4GKE7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_3181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000064">
        <property type="protein sequence ID" value="EHS85439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122529.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000064.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3181"/>
    <dbReference type="EnsemblBacteria" id="EHS85439">
        <property type="protein sequence ID" value="EHS85439"/>
        <property type="gene ID" value="PS3_3181"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1449"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="69" mass="7970" checksum="E92EC50B76402EE3" modified="2012-04-18" version="1">MSQSNLEKQEAKLKALNQKIKNEKNKIEQRLGKQIISQANLDYANLSNDQIKLLAKQFSEFLKVKSVDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKG2</accession>
    <name>H4GKG2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_5363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000066">
        <property type="protein sequence ID" value="EHS85397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122544.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000066.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5363"/>
    <dbReference type="EnsemblBacteria" id="EHS85397">
        <property type="protein sequence ID" value="EHS85397"/>
        <property type="gene ID" value="PS3_5363"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1481"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13673">
        <property type="entry name" value="Acetyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="145" mass="16715" checksum="FE3D6813AB0D4848" modified="2012-04-18" version="1">MEWKQKKFNELTTKELFQIYQFRSQVFTVGQNRIYQDPDENDLVATHVFAINNSDVVAYARIFIEDNFVTFGRVATSPSYRGQGLGQQLMDLVMQTIQQNYPKKPIEIHAQDYVEGFYQKFGFTSQGAPVQIAGTPHIKMTHPAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKH5</accession>
    <name>H4GKH5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methionine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000067">
        <property type="protein sequence ID" value="EHS85389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122557.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000067.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13016"/>
    <dbReference type="EnsemblBacteria" id="EHS85389">
        <property type="protein sequence ID" value="EHS85389"/>
        <property type="gene ID" value="PS3_13016"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1513"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Meth_synt_2" evidence="1">
        <location>
            <begin position="9"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Meth_synt_2" evidence="1">
        <location>
            <begin position="162"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="370" mass="41432" checksum="D1D1F88AB1FE9216" modified="2012-04-18" version="1">MTKLHYDIVGSFLRPAALKTAREQFEHGQIDHDQLKQVEDQEIKKLVQKEIEVGLKVVSDGEFRRSYWHLDTFWGFGGVKHTVPENGYFFHDEETRADSAQVAGKISFQPGHPDVEAFKFLKTLTDGTNVTPRQSIPAPAQFYAELVRGEENIAAIKRYYDQEADLLADISKAYRDLILALYDAGARDIKLDDCTWGMVVDDEFWATMASAGFDRDALQDTYLKVNNDALVDLPADLRISTHICRGNYHSTWAAQGGYAPVASHLFAKEKVDAFYLEFDDDRSGDFAPLAEVPAGKEVVVGLVTSKKPELEDPATLKARVQEASQYVALEDLALSTQCGFASTEEGNKLTEDQQWAKIKLVIDTAKEIWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKH6</accession>
    <name>H4GKH6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methionine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_16004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000067">
        <property type="protein sequence ID" value="EHS85390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16004"/>
    <dbReference type="EnsemblBacteria" id="EHS85390">
        <property type="protein sequence ID" value="EHS85390"/>
        <property type="gene ID" value="PS3_16004"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1514"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Meth_synt_2" evidence="1">
        <location>
            <begin position="9"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Meth_synt_2" evidence="1">
        <location>
            <begin position="164"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="369" mass="41606" checksum="7A45E0C467C87BF9" modified="2012-04-18" version="1">MTNLHYDIVGSFLRPEALKKAREQFEQGQIDHDQLKQVEDQEIQKLVQKEVEAGLKVVSDGEFRRSYWHLDTFWGFDGVDHTQAEHGYFFHDEETRADSATVVGKIKFNPQHPDLLAFKFLKSITPTGIVPRQSFPSPAQLYFELVRDERNVKALEQVYPNTDELLSDISNAYRDLILALYDAGARDIKLDDCTWGVLVDESFWQGPGSEIFDRADLQSKFLQLNNDALVDLPADLRISTHVCRGNFHSTWASSGGYAPVAKDLFAKEKVDAFYLEFDDDRSGDFAPLVEVPAGKEVVIGLVTSKKPELEDPAVLKARVKEASQYVALEDLALSTQCGFASTEEGNKLTEAQQWAKIKLVIDTAKEIWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKI0</accession>
    <name>H4GKI0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">UDP-galactopyranose mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_11350</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UDP-galactopyranose/dTDP-fucopyranose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122562.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11350"/>
    <dbReference type="EnsemblBacteria" id="EHS85260">
        <property type="protein sequence ID" value="EHS85260"/>
        <property type="gene ID" value="PS3_11350"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1521"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008767">
        <property type="term" value="F:UDP-galactopyranose mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004379">
        <property type="entry name" value="UDP-GALP_mutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015899">
        <property type="entry name" value="UDP-GalPyranose_mutase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03275">
        <property type="entry name" value="GLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00031">
        <property type="entry name" value="UDP-GALP_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GLF" evidence="4">
        <location>
            <begin position="148"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="373" mass="43637" checksum="43F7DD071A3A729D" modified="2012-04-18" version="1">MTQYDYLVVGAGLFGATFAYEAAQRGKRVKVIEKRDHLAGNIYTKEEFGIQVHQYGAHIFHTSNKEVWNYVQKFAEFNRYTNSPVANYNGKMYNLPFNMNTFSEMWGVRTPQEAIDKINEQRQEMAGKEPQNLEEQAISLIGRDIYEKLIKGYTEKQWGRKATELPAFIIRRLPVRLTYDNNYFNDDHQGIPKGGYTQIVEKMLASDLIDVETGVDFFDQKDQYLEKFPKVVFTGMIDQFFNYELGELEYRSLKFETETLDEGNYQGNAVVNYTDAETPYTRIIEHKHFEFGKGDENKTVITREYPADWQRGDEPYYPVNNDKNNQLYQQYQNYAAEQAKNVIFGGRLGQYKYYNMDQVIAAALETVQQEFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKI6</accession>
    <name>H4GKI6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome hibernation promoting factor</fullName>
            <shortName evidence="2">HPF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hpf</name>
        <name evidence="4" type="ORF">PS3_5373</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for dimerization of active 70S ribosomes into 100S ribosomes in stationary phase; 100S ribosomes are translationally inactive and sometimes present during exponential growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with 100S ribosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HPF/YfiA ribosome-associated protein family. Long HPF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122568.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5373"/>
    <dbReference type="EnsemblBacteria" id="EHS85266">
        <property type="protein sequence ID" value="EHS85266"/>
        <property type="gene ID" value="PS3_5373"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1530"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044238">
        <property type="term" value="P:primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00552">
        <property type="entry name" value="RaiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00839">
        <property type="entry name" value="HPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034694">
        <property type="entry name" value="HPF_long/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036567">
        <property type="entry name" value="RHF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003489">
        <property type="entry name" value="RHF/RaiA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032528">
        <property type="entry name" value="Ribosom_S30AE_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038416">
        <property type="entry name" value="Ribosom_S30AE_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16321">
        <property type="entry name" value="Ribosom_S30AE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02482">
        <property type="entry name" value="Ribosomal_S30AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69754">
        <property type="entry name" value="SSF69754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00741">
        <property type="entry name" value="yfiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Ribosom_S30AE_C" evidence="3">
        <location>
            <begin position="123"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="180" mass="20905" checksum="84D73EFB9FAAF423" modified="2012-04-18" version="1">MLKFNIRGENIEVTDAIREYVEKRLSKLEKYLEENVPAEAHVNLKVYSNKTYKVEVTIPLPYLTLRAEETSNDMYGSIDLVTDKLERQVRKYKTKVNRKAREKGLKDLEFVDTVDDEEPVSDLKIVRTKQISLKPMDSEEAVLQMDMLGHDFFVFEDADTNGTSIVYRRNDGCYGLIEVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKJ3</accession>
    <name>H4GKJ3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribonuclease BN</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_5132</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122575.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5132"/>
    <dbReference type="EnsemblBacteria" id="EHS85273">
        <property type="protein sequence ID" value="EHS85273"/>
        <property type="gene ID" value="PS3_5132"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1537"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017039">
        <property type="entry name" value="Virul_fac_BrkB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30213">
        <property type="entry name" value="PTHR30213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03631">
        <property type="entry name" value="Virul_fac_BrkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF035875">
        <property type="entry name" value="RNase_BN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00765">
        <property type="entry name" value="yihY_not_rbn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="298" mass="33556" checksum="636DF7F7589C88AB" modified="2012-04-18" version="1">MRNARHTVKRIIRTYLYHFQKARLSASAAELAYYTLLSIFPAILIVGNILPMVGLDAKTVLSYLETAIPTSVYNLISPVIYDLLHRGSGGLLTTGVVIAIWSTSQAIAAFQRDVNAAYGVAEDLNPIWNRIVSFLWILVVLVVIATLVFLYGLGEPILRNLQPILNFNTRYIHQFASYRYPVTFIGLFVGLLCLYYFVPNARVRLRFVALGSLVATFLLMGLARLLTYYGVLFNTQIASYKTISAFIVMMIWLDLSGIIVMIGATINATTQVLVEGKVQQRRYLWDFIKDKSNPDQPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKK4</accession>
    <name>H4GKK4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleotide-binding protein PS3_6958</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_6958</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Displays ATPase and GTPase activities.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RapZ-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000070">
        <property type="protein sequence ID" value="EHS85231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122586.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000070.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6958"/>
    <dbReference type="EnsemblBacteria" id="EHS85231">
        <property type="protein sequence ID" value="EHS85231"/>
        <property type="gene ID" value="PS3_6958"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1558"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00636">
        <property type="entry name" value="RapZ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005337">
        <property type="entry name" value="RapZ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30448">
        <property type="entry name" value="PTHR30448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03668">
        <property type="entry name" value="ATP_bind_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005052">
        <property type="entry name" value="P-loopkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="62"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="289" mass="32572" checksum="C3F75F0695B72030" modified="2012-04-18" version="1">MDQQLEVVVITGMSGAGKTVAVQSFEDSGYFVIDNMVPSLAPRFIDVIEESHQFSKIAMVMDARSQSFYDSVLPLIHQLRAREDITMRLLFLDADDVKLVARYKETRREHPLSENDGVMSGIQRERQLLATMKSEADLVVDTTTLTPRQLRLRLQDYFGDGEADQFYINVMSFGFKYGLPLDADIVMDVRFLPNPFYLPELKHLTGNDQAVQDYVMQTSEAQAFYRHFLALLMTTLPGYIKEGKSSLTIAIGCTGGQHRSVTMANRLSTALSEAGYQASVHHRDVEKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKL3</accession>
    <name>H4GKL3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">Phosphoglucosamine mutase</fullName>
            <ecNumber evidence="6 8">5.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">glmM</name>
        <name evidence="13" type="ORF">PS3_6956</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23424"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="5.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="6">Activated by phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.10" evidence="6 8"/>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122595.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6956"/>
    <dbReference type="EnsemblBacteria" id="EHS85182">
        <property type="protein sequence ID" value="EHS85182"/>
        <property type="gene ID" value="PS3_6956"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1570"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05802">
        <property type="entry name" value="GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01554_B">
        <property type="entry name" value="GlmM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006352">
        <property type="entry name" value="GlmM_bact"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01455">
        <property type="entry name" value="glmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 6 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGM_PMM_I" evidence="10">
        <location>
            <begin position="4"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_II" evidence="11">
        <location>
            <begin position="161"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_III" evidence="12">
        <location>
            <begin position="260"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_IV" evidence="9">
        <location>
            <begin position="376"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="6">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="6">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="6">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS85182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="448" mass="48543" checksum="CA005E58C91424D7" modified="2012-04-18" version="1">MDLKYFGTDGVRGVANQDLSPELAFQVGRAGGYVLTRHSERKQPQVLVARDTRISGQMLENALVAGLLSVGIEVLRLGVVTTPGVAYLVRAQEADAGVMITASHNPIEYNGIKYFGADGFKLSDDLEYEIEQLLDAPVDELPRPSADGLGMVDDYLEGSLKYTSFLEQTISTDLSGLKVVVDAANGATSAFISPLFADLNVDLIPINDQPDGLNTNLDCGSTHPEGLQKAVLEHEADLGLAFDGDGDRCIAVDEHGQLVDGDKIMYICGRYLEQNGRLKHNTVVTTVMSNLGMYKALEAHDMQSVKTKVGDRYVVEEMLANGYNLGGEQSGHIIFLDHNTTGDGMLTALQLLQVVKATGQTLSELASEVKTYPQELVNITVQDKQAALSNPELKAVIEEVEQEMAGDGRVLVRPSGTEPLLRIMAEAPTEELVHQYVTKIAEVARRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKN9</accession>
    <name>H4GKN9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Protein GrpE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">HSP-70 cofactor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">grpE</name>
        <name evidence="7" type="ORF">PS3_852</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 5">Belongs to the GrpE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122621.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_852"/>
    <dbReference type="EnsemblBacteria" id="EHS84959">
        <property type="protein sequence ID" value="EHS84959"/>
        <property type="gene ID" value="PS3_852"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1599"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_coiled_coil"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="PTHR21237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="SSF51064"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="SSF58014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01071">
        <property type="entry name" value="GRPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS84959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="186" mass="20758" checksum="4C38C2EF577CDD61" modified="2012-04-18" version="1">MAKEEQTQDQSTQAPADEVETTTEASEQVEDQVAELSQQVADLQAKLTEAEDKELRAQAEVQNMSKRMKKEQAQLLKYEGQDLAKSLLPVLDNLKRALAIEVTDENGQQLKKGIQMVHDHLETALTDHDVHEVDALNHPFDPNTQQAVQTVPAQEGQEPETVVEVLQAGYTLHDRVLRPAMVVVCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKQ4</accession>
    <name>H4GKQ4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-dehydropantoate 2-reductase</fullName>
            <ecNumber evidence="5">1.1.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ketopantoate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_19206</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5">Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>(R)-pantoate + NADP(+) = 2-dehydropantoate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:16233"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.169"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 5">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.169" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122636.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19206"/>
    <dbReference type="EnsemblBacteria" id="EHS84974">
        <property type="protein sequence ID" value="EHS84974"/>
        <property type="gene ID" value="PS3_19206"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1625"/>
    <dbReference type="UniPathway" id="UPA00028">
        <property type="reaction ID" value="UER00004"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ApbA" evidence="6">
        <location>
            <begin position="3"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="ApbA_C" evidence="7">
        <location>
            <begin position="187"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="314" mass="34768" checksum="1CD876BAB4481B1B" modified="2012-04-18" version="1">MKVLVAGVGGMGTRFALCLAHAGNDVVVADGWQDRLSYLKKDGLKAKFNGQEVQVRVPAYAQTEVPTDQHFDVVLFLCKSMQLDQMVQDVLPAFDDNTFALCLMNGVGHEKTLAKYVDPDRVLLGNTMWTAQMTRPDHVFLGDDGSCELGSPNGNPASQKMAKQVVEVFSQAGLKAHYLDNPRYSVFRKGCVNGTLNTLCTLLECNLTEYSQTPGAHDMVVKIVDEFAAVDEKEGVNLDRQEVVDHVEASYDVIGDHYPSMYQDLIKNNRVTEIDYMNGYVSTHGHKYGLATPYCDLLTDLVHAKESVRHANKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKQ7</accession>
    <name>H4GKQ7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="3 15">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PS3_18376</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>(R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + NAD(+) = (R)-N(6)-lipoyl-L-lysyl-[protein] + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 15">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="13 15">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="15">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="3 15"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122639.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18376"/>
    <dbReference type="EnsemblBacteria" id="EHS84977">
        <property type="protein sequence ID" value="EHS84977"/>
        <property type="gene ID" value="PS3_18376"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1630"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006258">
        <property type="entry name" value="Lipoamide_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01350">
        <property type="entry name" value="lipoamide_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 13 15" id="KW-0274">FAD</keyword>
    <keyword evidence="5 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9 13 15" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11 15" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="17">
        <location>
            <begin position="4"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="16">
        <location>
            <begin position="331"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="429"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="137"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="174"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="302"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="14">
        <location>
            <begin position="41"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EHS84977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="450" mass="48797" checksum="704ED0A740A60DFD" modified="2012-04-18" version="1">MANYDVLVIGAGPGGYVAAEEAARLGKTVAVIEKKAIGGACLNVGCIPSKTYLQHSQWLLTFDQARQYGIEIQVQQLDFAKLHAHKDQVVASLQNGIRTTFKQAKIDYFAGEAQYFKGTTFLVDGERITGKDVILATGSHPFVPQINGIDQVNYLTTDEFFDLATLPKRFVVIGGGVIAIELAFAMRPLGVEVTVIEVAPEILLTEDPVAREIIRQKLKDLGVQIFEGAQIANVKPEQVELVGESPQRFDQLLVATGRKPNTDLAKAIGLELTDRGFVAVDQYYESSKAHFYAIGDLLPTYMLAHVASAEGIKAVRAICRQAEDPVDADAIPRSLYTSPEVASFGMNEEAARAAGFDVVTGQLPFAYNGRAIATAKTDGFIRLISEKQYHLILGALIVGDHATDLLQELILLKNAEGTLDQIVDSVYAHPTISELIQDVAKQVIAKGARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKQ8</accession>
    <name>H4GKQ8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ATP-dependent Clp protease ATP-binding subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_15929</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15929"/>
    <dbReference type="EnsemblBacteria" id="EHS84978">
        <property type="protein sequence ID" value="EHS84978"/>
        <property type="gene ID" value="PS3_15929"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1631"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="4">
        <location>
            <begin position="30"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="4">
        <location>
            <begin position="301"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="ClpB_D2-small" evidence="5">
        <location>
            <begin position="472"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS84978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="564" mass="63216" checksum="F8F7152ED9EBB57E" modified="2012-04-18" version="1">MQELQADSDLPVIGRQSELTDLIRVLNRQTKNNALLVGPPGVGKRTIIRDLIKHLDQTKATKIYRLDLGQLVAGTKYRGEFEERFTKLLNNLQELDEKIILYVDDLPGLTTIGQADGAISAAEMLKPILEAGAIQLITSMTHAEFRESLDPDPALARHFQRITIEEPNEREVTEILAVWEDRFAAYHGVSYQWDCIRVAINLSNRYLSERALPDKALDLLDEAGAMQGNDGSQKVTPDDLAYIVERHTGVKVRGVMATERDHLLNLVPMLHAKIIGQDEAVEKVANAIIRSRAGIQNPQRPIGSFLFLGPTGVGKTALAKRLAEVLYGDQNEMIRIDMSEYMEKHAVARLVGPPPGYVGYEEGGQLTEAVRQRMSAVILLDEIEKAHPDVFNLLLQLLDEGRLTDAKGRTIDFKNTILIMTSNLGSTKILESLEENGELVSSTRNSVLEDLKHQFRPEFLNRIDDIILFNPLTSTDAEQIVKLMLAELTERLQNQHVNLEFDPAVVTYLAHVGFDPVFGARPLQRTIVQMIETPLAKKIIRQTRDSSSTYYITQDQSGFTINQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKV8</accession>
    <name>H4GKV8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Histidinol-phosphatase</fullName>
            <shortName evidence="7">HolPase</shortName>
            <ecNumber evidence="4 7">3.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_6132</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H2O + L-histidinol phosphate = L-histidinol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14465"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="3.1.3.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 8/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PHP hydrolase family. HisK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.15" evidence="4 7"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122690.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6132"/>
    <dbReference type="EnsemblBacteria" id="EHS85028">
        <property type="protein sequence ID" value="EHS85028"/>
        <property type="gene ID" value="PS3_6132"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1690"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00013"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004401">
        <property type="term" value="F:histidinol-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010140">
        <property type="entry name" value="Histidinol_P_phosphatase_HisJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21039">
        <property type="entry name" value="PTHR21039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01856">
        <property type="entry name" value="hisJ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHP" evidence="8">
        <location>
            <begin position="5"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS85028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="276" mass="31960" checksum="EBF395DC5C211A57" modified="2012-04-18" version="1">MKIEGHSHTELCPHGSGDRLEDMVKKAIQMGFTDYCVTEHAPLPNGFVAEFGGVEDDWQTASLTYEQVESYFNLVDQVRAKYQDQIKLHVGFEVDYVDGFEAEIRQFLDTYGPRTDQNILSVHYLRGADGLIYGVDYSPEELQMGFTDLEDQPHAFYQRYLRVIKQAISTDLGVYQPQRLGHLTLMRKYQDYFALPQDYNEQEWRLIKNILILLKDQGRQLDFNLAGMYKEYCNDFYPGTRITKLAKELGVSMVYGSDAHSIKEVGHGYHLYEAFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKW3</accession>
    <name>H4GKW3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ribonuclease HI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_8992</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122695.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8992"/>
    <dbReference type="EnsemblBacteria" id="EHS85033">
        <property type="protein sequence ID" value="EHS85033"/>
        <property type="gene ID" value="PS3_8992"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1697"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="130" mass="14292" checksum="E36F1BB8B3B440FF" modified="2012-04-18" version="1">MIKIYSDAAVSGNPGPAGAGCLIIINGQQHQFSHSLPATDNHHAEFMAASWAFEQLAQFKSADQIIFFYTDSRLVSDAIGKGYAKKYAAELSRLLTLIDQYPTVVTEWLPEKLNHGAHQLALQALHQSHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKW9</accession>
    <name>H4GKW9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative mannosyl-glycoprotein endo-beta-N-acetylglucosamidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_6356</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 73 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000073">
        <property type="protein sequence ID" value="EHS84943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6356"/>
    <dbReference type="EnsemblBacteria" id="EHS84943">
        <property type="protein sequence ID" value="EHS84943"/>
        <property type="gene ID" value="PS3_6356"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1705"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002901">
        <property type="entry name" value="MGlyc_endo_b_GlcNAc-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01832">
        <property type="entry name" value="Glucosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00047">
        <property type="entry name" value="LYZ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="LYZ2" evidence="4">
        <location>
            <begin position="283"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS84943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="449" mass="50548" checksum="50162C5A76B9F4ED" modified="2012-04-18" version="1">MDTTQTSDTSNDVQTNSVTLQSGSDSTSASNVNTTGSDNSSEQSSQSNSNSNSTVHAPLLLYSVQTNNDSTTKNGVVSEDGSNYYYVNGQKQTDYFYTNDSGQVYYFGDDGKMYQDQFYSNWGNMYYFGEDGARYTDQFYSNWGNMYYFGDDGVRYTNQFYSNWGNVYYFGEDGVRYTNQFYSNWGNLYYFGEDGARYTNQFYSNWGNTYYFGEDGVRYTNQFYSNWGNLYYFGDDGVRLDNGFYYAWGNVYYFKNDGARATNETVSVDGVGDLYFDNDGVLAYDASGTSQNEFISSIVNGAIEGWVDYGVLPSLTIAQAIIESAWGKSSLSATYHNLFGIKAGSSWTGATVNMSTGEYYNGSYTTIVDAFRAYSSNNDSVKDHSLFLVNNSRYSNLLWDKSSDSVTAKIYQDGYATSPTYTSTLRNVISNYGLTVFDQVAFKIKDMGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKX6</accession>
    <name>H4GKX6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dTDP-4-dehydrorhamnose reductase</fullName>
            <ecNumber evidence="2">1.1.1.133</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_15826</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>dTDP-beta-L-rhamnose + NADP(+) = dTDP-4-dehydro-beta-L-rhamnose + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21796"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57510"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62830"/>
            <dbReference type="EC" id="1.1.1.133"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the dTDP-4-dehydrorhamnose reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.133" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000075">
        <property type="protein sequence ID" value="EHS84840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122708.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000075.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15826"/>
    <dbReference type="EnsemblBacteria" id="EHS84840">
        <property type="protein sequence ID" value="EHS84840"/>
        <property type="gene ID" value="PS3_15826"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1716"/>
    <dbReference type="UniPathway" id="UPA00124"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008831">
        <property type="term" value="F:dTDP-4-dehydrorhamnose reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019305">
        <property type="term" value="P:dTDP-rhamnose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005913">
        <property type="entry name" value="dTDP_dehydrorham_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029903">
        <property type="entry name" value="RmlD-like-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10491">
        <property type="entry name" value="PTHR10491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04321">
        <property type="entry name" value="RmlD_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01214">
        <property type="entry name" value="rmlD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RmlD_sub_bind" evidence="3">
        <location>
            <begin position="3"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU364082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS84840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="281" mass="32359" checksum="FD961FF8877C593C" modified="2012-04-18" version="1">MTEIMITGAKGQLGTELCHLLDERGITYDAFDSKSLDITDKELVDRKVKELKPRVIYHCAAYTAVDKAEDDGKEINWSVNVDGTRNITKAAQDVEATLVYISTDYVFDGTKDSEYDVDDDPNPKNQYGKAKLAGEQAVQEIMDRYYIIRTSWVFGQYGNNFVYTMLRLAKDHDQLTVVSDQFGRPTWTKTLAEFMTYLIDRNTQFGIYQLSNEGTCSWHEFAKEILKDENVKVLPVTSEEYPQKAYRPRHSVMSLQKTKATGFKIPTWKEALYEFTKKNRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKZ1</accession>
    <name>H4GKZ1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_12861</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nlpA lipoprotein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122723.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12861"/>
    <dbReference type="EnsemblBacteria" id="EHS84757">
        <property type="protein sequence ID" value="EHS84757"/>
        <property type="gene ID" value="PS3_12861"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1734"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004872">
        <property type="entry name" value="Lipoprotein_NlpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30429">
        <property type="entry name" value="PTHR30429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03180">
        <property type="entry name" value="Lipoprotein_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002854">
        <property type="entry name" value="MetQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS84757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="281" mass="31275" checksum="E5B36B9FBC4C9B73" modified="2012-04-18" version="1">MHKKRNWTIGIIIAVVVVIGAYFSFFHHSQTSQKTVTVGVVDSSKADVAIWKQVAKTAKEKYGITVKTKTFTDYNQPNKALKNGDIDLNAFQHQAFLDEWNKANKGNLVSIGKTVIAPIRLFSTKYKSINDLPDGATIAVPNDATNESRALYVLKNAGLITFKKGTGKLATISDIKTNKKNLNIKEMDASQTARTSDDVDAAVVNNTYANAAKLSSKDVIYTEPVNKDSEQWINIIVARKQDKNNQAYKDVVKAYQTAAVKKLYQKYYGDTEKTAWDLNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL10</accession>
    <name>H4GL10_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ABC transporter substrate-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_15021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122742.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15021"/>
    <dbReference type="EnsemblBacteria" id="EHS84776">
        <property type="protein sequence ID" value="EHS84776"/>
        <property type="gene ID" value="PS3_15021"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1758"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06325">
        <property type="entry name" value="PBP1_ABC_unchar_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007487">
        <property type="entry name" value="ABC_transpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35271">
        <property type="entry name" value="PTHR35271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04392">
        <property type="entry name" value="ABC_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS84776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="330" mass="35127" checksum="C123F3F0EA389673" modified="2012-04-18" version="1">MKRLYTIIGILVAVVIIALVGNQLSQRSNSTKPTVGILQLMSHPSLNQIHKGVIAGLKEEGYVNGKNIKIDFQNAENDQSNLKSMAEKFKNENAKVTIGITTQAVQALANASSKTPIVMGAVSDPVGSGIVKSLKHTGNNVTGVQHVEPVAQQAKLIRTLLPNVKTIGVIYNAGDDAATGEVKLFQSLMEKQGITVKTYTITSTNDIDQVSANMASEVEAVYVPTDNTVAAGIQTLLKNTDSANVPVFGSVQSMIKSGAVATYSISQYDMGVLTGKMAGQILKGKKPSAMPVQHVKTGHYYINTTAAKKFNITIPQSILNSAKKDGVIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL33</accession>
    <name>H4GL33_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase/oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_17113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122765.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17113"/>
    <dbReference type="EnsemblBacteria" id="EHS84663">
        <property type="protein sequence ID" value="EHS84663"/>
        <property type="gene ID" value="PS3_17113"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1787"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="277" mass="30686" checksum="9C87C37CCFFB4D98" modified="2012-04-18" version="1">MLRRLKSLRSLKNKVVLITGGSQGIGEALANEAARRGAIVVVCARNVEKLELVAKRCMILSGRPSFHYQLDVTDPDAVDRVLTIIQHEVGAIDVLVNSAGLGDMVAVVKQKYTSMSKMVQVNLLATMYLSRKVAKQMMDQGYGAIINIASLAGKIPTPHSGVYSATKAGIVQFDNILRMEVADYGVQVLTVNPGPVKTEFFQKADPTGNYLAMLPEQLQIEPEQLASQVWDNVGHKTREINVPAYTTALSWVYNLIPGLGDWWIKRLFNYQQDKWKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL38</accession>
    <name>H4GL38_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable GTP-binding protein EngB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">engB</name>
        <name evidence="10" type="ORF">PS3_722</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Necessary for normal cell division and for the maintenance of normal septation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122770.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_722"/>
    <dbReference type="EnsemblBacteria" id="EHS84668">
        <property type="protein sequence ID" value="EHS84668"/>
        <property type="gene ID" value="PS3_722"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1793"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00321">
        <property type="entry name" value="GTPase_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019987">
        <property type="entry name" value="GTP-bd_ribosome_bio_YsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03598">
        <property type="entry name" value="GTPase_YsxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0717">Septation</keyword>
    <feature type="domain" description="EngB-type G" evidence="9">
        <location>
            <begin position="22"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS84668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="197" mass="22290" checksum="1AF47CBEAD418EFB" modified="2012-04-18" version="1">MQVHEVELTISAVKAEQYPATNYPEIALVGRSNVGKSSLTNVLINRKNYAHTSGQPGKTQTLNFYEVEHQLYFVDVPGYGYAKVSKKQREQFGKMIEHYLTEREQLRGVVQLIDGRHAPTEDDVMMYQWLAYYQIPTLVVATKIDKVKSSAYTKSISSITKGLGLDQSVPLVLFSAQTKRGAQEVWDWIEKQTGVTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL39</accession>
    <name>H4GL39_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent Clp protease ATP-binding subunit ClpX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">clpX</name>
        <name evidence="9" type="ORF">PS3_9745</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the ClpX-ClpP complex. Forms a hexameric ring that, in the presence of ATP, binds to fourteen ClpP subunits assembled into a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the ClpX chaperone family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122771.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9745"/>
    <dbReference type="EnsemblBacteria" id="EHS84669">
        <property type="protein sequence ID" value="EHS84669"/>
        <property type="gene ID" value="PS3_9745"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1794"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00175">
        <property type="entry name" value="ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004487">
        <property type="entry name" value="Clp_protease_ATP-bd_su_ClpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046425">
        <property type="entry name" value="ClpX_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010603">
        <property type="entry name" value="Znf_CppX_C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038366">
        <property type="entry name" value="Znf_CppX_C4_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06689">
        <property type="entry name" value="zf-C4_ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00994">
        <property type="entry name" value="zf-C4_ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00382">
        <property type="entry name" value="clpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51902">
        <property type="entry name" value="CLPX_ZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ClpX-type ZB" evidence="8">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="119"/>
            <end position="126"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS84669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="415" mass="45683" checksum="6E64B9F5FB206355" modified="2012-04-18" version="1">MFEDTNGQDIHCSFCGKSQADVAKIVAGPGVYICNECVALCQEIIDQELAEERSGQQTFKVLTPAEIKAQLDDYVIGQEDAKKTLSVAVYNHYKRVNAMIEGENNDTELQKSNIAVIGPTGSGKTYLAQSLARILNVPFAIADATTLTEAGYVGEDVENIILKLLQAADFDIDRAEKGIIYIDEIDKIAKKSENVSITRDVSGEGVQQALLKILEGTIANVPPQGGRKHPQQEFIQVDTSNILFIVGGAFDGIETIVKERLGDKTIGFGTNSREIDTVTDKNVLQHVVPEDLLKFGLIPEFIGRLPVMTALEKLDEDDLVRILTEPKNALVKQYQTLIGLDDCELEFTDGALKAMAQLAIERQTGARGLRSIIEDVMRDVMYDLPSRQDVAKVVIEKRCVTQHTAPRYVLKQEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL56</accession>
    <name>H4GL56_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative inositol-phosphate phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_3989</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3989"/>
    <dbReference type="EnsemblBacteria" id="EHS84686">
        <property type="protein sequence ID" value="EHS84686"/>
        <property type="gene ID" value="PS3_3989"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1812"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS84686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="254" mass="28159" checksum="1428105A60D6B303" modified="2012-04-18" version="1">MLAEIDQQIQTLIREVGERTKAAMQADFTIDEKTSPHDLVTTVDKQNEQIINQRLREIDPTAQIISEEGFGDQVHDIHGHVWIVDPVDGTMNFIMERENFAIMLALYIDGQPTLGYILDVMKGLLYHGGPALGVWEDDHQLSTPLNRGLRDGLIDVSSRILMNDLHHAQTAAKAARSIRVFGSAGISMTQLITGRTGAYITNLKPWDLAPGRVLAEAQGLVVKRIDEQPIDMLSSNFVLVATPKAAKDIMELIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL71</accession>
    <name>H4GL71_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cell division protein SepF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">sepF</name>
        <name evidence="6" type="ORF">PS3_5620</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Cell division protein that is part of the divisome complex and is recruited early to the Z-ring. Probably stimulates Z-ring formation, perhaps through the cross-linking of FtsZ protofilaments. Its function overlaps with FtsA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. Interacts with FtsZ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">Localizes to the division site, in a FtsZ-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SepF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5620"/>
    <dbReference type="EnsemblBacteria" id="EHS84701">
        <property type="protein sequence ID" value="EHS84701"/>
        <property type="gene ID" value="PS3_5620"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1834"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01197">
        <property type="entry name" value="SepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023052">
        <property type="entry name" value="Cell_div_SepF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007561">
        <property type="entry name" value="Cell_div_SepF/SepF-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038594">
        <property type="entry name" value="SepF-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35798">
        <property type="entry name" value="PTHR35798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04472">
        <property type="entry name" value="SepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 5 6" id="KW-0132">Cell division</keyword>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0717">Septation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS84701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="144" mass="15934" checksum="4DF87C15CBB250ED" modified="2012-04-18" version="1">MANFFSNLFGVDDEYNEQEEYYDQTTSTNATTNVRNSKVVPFDSSNRSSTSTPNSQIDLVEPRLYGDAREIATQLLDGKAVIVNFSQMDANTAVKVVDFLNGAIFAIDGEIKRIGEQIFLCTPRNFEVAGKLAATLKNMGQSEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL91</accession>
    <name>H4GL91_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_15776</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000079">
        <property type="protein sequence ID" value="EHS84620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15776"/>
    <dbReference type="EnsemblBacteria" id="EHS84620">
        <property type="protein sequence ID" value="EHS84620"/>
        <property type="gene ID" value="PS3_15776"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1859"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028259">
        <property type="entry name" value="AP2-like_int_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14657">
        <property type="entry name" value="Arm-DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14659">
        <property type="entry name" value="Phage_int_SAM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="5">
        <location>
            <begin position="61"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="4">
        <location>
            <begin position="168"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS84620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="369" mass="42638" checksum="557D020A4AFFE17B" modified="2012-04-18" version="1">MEEKSMASITKRGKVWQARITWMDSSGKRHSKSKSGFKTKSQANQWSVQQESVLVSGVQIDKDSTLVDYFDHYIEIYKRPHVTESTYKGYGFIRRQLVNFFQYRKIKDITRTDYQEFINSYGSDHAPKSVQKLHSIMRGCVQSAILDDYIIKDFTQGVQVVANKDKMVKVDYLNLDEIKRLLNYTIDGINPLRRYTSRYIIVTAIYTGMRLAEIQGLVWSDIDYLHETITISKTWDSVNHRFKPTKTESSNRTIKVPDNLIKLLRQLQAVSSSTMVFVDQFGQIPTSNAVNKLLRRLLDELGIKRANFHFHSLRHSHVAILLSQGVDIYAISSRLGHSNISTTTDTYAYLIDEYKDKTDDHIISALDQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLA6</accession>
    <name>H4GLA6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase M16 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13438</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122838.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13438"/>
    <dbReference type="EnsemblBacteria" id="EHS84605">
        <property type="protein sequence ID" value="EHS84605"/>
        <property type="gene ID" value="PS3_13438"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1883"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M16_C" evidence="1">
        <location>
            <begin position="181"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="416" mass="46960" checksum="3605E9D0F10DFEA9" modified="2012-04-18" version="1">MDKQIMAGVHLHVIPTHQFQTTKIMMTFTSRQTATNAAVRNLLTNILSTSSAKYPTQTALARQLSNLYGAMIGAYVSRLSYAHVMRFKISVINERYTDQPLLPQGFDLLKELIFNPLMNADGFDALTFKLQQENLINTLKSWDDDKQFYALRQVEELVFQNDPVLATPSTGTVDQVAELTNLDLMRAYQDMIAKDRIDIYVVGDVDAAEVEQLVSQLPLKERQFNQFAVLTRPNWQGDVLEKVEHQPIEQAKLDRVYLCPVNYREPLYRAAMVMNGLLGGTPYSFLFTNVREQASMAYYASSSLRPFNGHLVMQTGIQPDNVTAVQTLLDRQVTLLQAGDFSLERLQMVKDALINQYETSQDYQNSLLERQLTATLTGIATANDFAKEIQAVTKDQVVEVAQGLSKCVEYVLTGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLB3</accession>
    <name>H4GLB3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Valine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Valyl-tRNA synthetase</fullName>
            <shortName evidence="9">ValRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">valS</name>
        <name evidence="14" type="ORF">PS3_5382</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val)</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The C-terminal coiled-coil domain is crucial for aminoacylation activity.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122845.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5382"/>
    <dbReference type="EnsemblBacteria" id="EHS84612">
        <property type="protein sequence ID" value="EHS84612"/>
        <property type="gene ID" value="PS3_5382"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1891"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02004">
        <property type="entry name" value="Val_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019499">
        <property type="entry name" value="Val-tRNA_synth_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="PTHR11946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10458">
        <property type="entry name" value="Val_tRNA-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 9 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1" evidence="11">
        <location>
            <begin position="19"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_1" evidence="12">
        <location>
            <begin position="611"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="domain" description="Val_tRNA-synt_C" evidence="13">
        <location>
            <begin position="817"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="815"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="9">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="9">
        <location>
            <begin position="528"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="531"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF10458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS84612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="882" mass="101544" checksum="8AB0DC60835B2F76" modified="2012-04-18" version="1">MSDQDMSTKYNPTEVEKDRYQWWIDQGFFKPSGDQKAHPYSIVIPPPNVTGKLHLGHAWDTTLQDIIIRQKRMQGYDVLWLPGMDHAGIATQAKVEARLAEQGISRYDLGREKFIQQVWDWKDEYADIIHQQWAKMGISVDYDRERFTLDDGLSKAVRKVFVDLYNKGLIYRATYIINWDPKARTALSDIEVEHQDDQGAFYHVKYPFTDGTTFNGQDYIEIATTRPETMFGDVAVAVNPDDDRYKELVGKHIRVPLVDREVEIIADDYVTPDFGTGMVKITPAHDPNDFQIGKRHDLPEINTMNEDASLNENAGKYEGMDRFDAREAIVKDLEDQGYILRIDPIVHSVGHSQRTGVQVEARLSTQWFVKMKPLAEQALQAQETDDRVDFFPPRFENTYRQWMENVHDWVISRQLWWGHRIPAWYHKETGEMYVGMEAPADSENWEQDPDVLDTWFSSGLWPFSTMGWPDTDSEDFKRYFPTTTLVTGYDIIFFWVSRMIFTSLEFTGRTPFKNVLLHGLIRDEEGRKMSKSLGNGIDPMDVIEKYGVDALRWFLVTGSTPGQDIRFSYKKMDAAWNFINKIWNASRYVIMNLDDMAAPSLPAQSEWTLADKWILSRLNATVSQVTSQFDKFEFGEAGRALYNFIWNDFCDWYIEMTKETLNNGSDQEKTNTKNILGYVLDQTLKLLHPIMPFMTEQVWQSMPHDGDSIMVAKFPEEHAEFSDPKAEDQIASLIELIKAVRNIRNEAGAPMSKPVDLMIKVDSAELGEIFNETKEYIDRFCHPAELKIGQDLTEPKLAMSGILAGATVYIPMAELVDLEEEKARMQKEIQKLEQEVARSEKKLGNEKFVANAPEAVVADEKAKAAEWQQKLAAAKERLAQLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHR7</accession>
    <name>H4GHR7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">PTS family mannose/fructose/sorbose porter component IIC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_14473</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000004">
        <property type="protein sequence ID" value="EHS87547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121601.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000004.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14473"/>
    <dbReference type="EnsemblBacteria" id="EHS87547">
        <property type="protein sequence ID" value="EHS87547"/>
        <property type="gene ID" value="PS3_14473"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.30"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004700">
        <property type="entry name" value="PTS_IIC_man"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03609">
        <property type="entry name" value="EII-Sor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51106">
        <property type="entry name" value="PTS_EIIC_TYPE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="268" mass="27353" checksum="185F66B8226F27D2" modified="2012-04-18" version="1">MSAISIVLVAIVAFLAGVEGILDEFQFHQPLVACTLIGLVTGHLIPCVMLGGSLQMIALGWANIGAAVAPDAALASIASAIIFVEGGANTSDIGTAIGLAVPLAVAGLFLTMIVRTISVAIVHVMDAEGAKGNLGRVDMWQIIAVCLQGLRIMLPAIALAFIPASTVKSFLASMPSWLTDGMTIGGGMVVAVGYAMVINMIATREVWPFFIIGFVIAAITQLTLIAIGALGVALMLLYLQLEGKGGSSNGSNGASGTGDPLGDILDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHS0</accession>
    <name>H4GHS0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Transcription termination/antitermination protein NusG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nusG</name>
        <name evidence="9" type="ORF">PS3_15371</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Participates in transcription elongation, termination and antitermination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the NusG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000004">
        <property type="protein sequence ID" value="EHS87550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121604.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000004.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15371"/>
    <dbReference type="EnsemblBacteria" id="EHS87550">
        <property type="protein sequence ID" value="EHS87550"/>
        <property type="gene ID" value="PS3_15371"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.38"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006354">
        <property type="term" value="P:DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00948">
        <property type="entry name" value="NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043425">
        <property type="entry name" value="NusG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001062">
        <property type="entry name" value="Transcrpt_antiterm_NusG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265">
        <property type="entry name" value="PTHR30265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265:SF2">
        <property type="entry name" value="PTHR30265:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02357">
        <property type="entry name" value="NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00338">
        <property type="entry name" value="NUSGTNSCPFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82679">
        <property type="entry name" value="SSF82679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00922">
        <property type="entry name" value="nusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5 6" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="3 5 6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 5 6" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="NGN" evidence="7">
        <location>
            <begin position="5"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="8">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="181" mass="20387" checksum="C1723A8CFF4465D2" modified="2012-04-18" version="1">METSEKQWYVLHTYSGYENRVKSNLESRVQSMNMEDYIFQVVVPEETVRQVKDGQAKEVEEKTFPGYVLVQMVMTDQAWYIARNTPGVTGFLGSHGGGSKPTPMLPEEVDRIMKRMGVEVARNDIDVEVGDQVKIIAGAFADLVGTVTEVDHDKMKLKVAIEMFGRETTADLGFNQVDTMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHS3</accession>
    <name>H4GHS3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Recombination ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_7517</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. RarA/MGS1/WRNIP1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121607.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7517"/>
    <dbReference type="EnsemblBacteria" id="EHS87479">
        <property type="protein sequence ID" value="EHS87479"/>
        <property type="gene ID" value="PS3_7517"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.41"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032423">
        <property type="entry name" value="AAA_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021886">
        <property type="entry name" value="MgsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16193">
        <property type="entry name" value="AAA_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12002">
        <property type="entry name" value="MgsA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="SSF48019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="50"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="445" mass="50510" checksum="94A334272D514795" modified="2012-04-18" version="1">MDQTSLFDQAMDENAPLASRVRPRDLDHFVGQEHLVGPGKFLREMIEKDQLSSMIFWGPPGVGKTTLAEIIARQTKSHFVTFSAVMNGIKEIRKIMQAAENDRQVGIRTIVFIDEIHRFNKAQQDAFLPYVEKGSIILIGATTENPSFEINSALLSRTRVFMLKPLSDQDLMKLLHQVLSEPQAFGEIKVSITDELLHEIAIYSNGDARSALNTLEMLVINSDMHDHQVTVDQNLLAELTDHKTQYYDKDGEEHYNIISALHKSMRNSDVDSAIYWLSRMLDGGEDPLYIARRLVRFASEDIGLADNNALNLAINTFQACQFLGLPECDVHLTQCVIYLALSPKSNATYQARVAVKKDIKETINEPVPMQLRNGTTKLMREMGYGKGYQLAHHYADKLTTIPTRPASINNHIYYQPTEQGNEGKIKQRLEYIKRWKASHDTNFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHS6</accession>
    <name>H4GHS6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA processing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_20221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DprA/Smf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121610.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20221"/>
    <dbReference type="EnsemblBacteria" id="EHS87482">
        <property type="protein sequence ID" value="EHS87482"/>
        <property type="gene ID" value="PS3_20221"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.45"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009294">
        <property type="term" value="P:DNA-mediated transformation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003488">
        <property type="entry name" value="DprA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43022">
        <property type="entry name" value="PTHR43022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02481">
        <property type="entry name" value="DNA_processg_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00732">
        <property type="entry name" value="dprA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="283" mass="31070" checksum="35B5FACCAB3C3E1B" modified="2012-04-18" version="1">MKLNDFIFRLSLCEHLGSVNQLKIIRAKIASPEAALATLLKTAGISSQQGQAILNQWFSPELDRRMRLNQTVPHLLITDEAYPAALKESYGAPTVLYYRGNRELLTTQMLAVVGARQMSSYGQMVMDQLLPPVIYAGITIVSGLARGVDGYSHHIAIDHAGKTIAVIGTGLDRFYPQEHRQLMQQMMQSQLVLTEYPLGTPPLASHFPARNRIIAGLCQSCLVVEGRQRSGSLITANLALQENRNVLAVPGAINHELSTGCNELIAEGAKPILRALDILEEYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHT2</accession>
    <name>H4GHT2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DegV family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13394</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121616.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13394"/>
    <dbReference type="EnsemblBacteria" id="EHS87488">
        <property type="protein sequence ID" value="EHS87488"/>
        <property type="gene ID" value="PS3_13394"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.51"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003797">
        <property type="entry name" value="DegV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043168">
        <property type="entry name" value="DegV_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02645">
        <property type="entry name" value="DegV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00762">
        <property type="entry name" value="DegV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51482">
        <property type="entry name" value="DEGV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="280" mass="30161" checksum="F2D3633F2010DC2E" modified="2012-04-18" version="1">MANIKLVTDSSAGLTDEEIKEYDITIVPLSVMIDDQVYVEREDITNDEFVAKMAESTNLPKTSQPPLGKFVEVFDRLGADGSQVLCIDMMASLSGTVHAAEQAATMTETDVTVIDSLTTDRALAYQLIEAAKAIKAGATMEEVVAKARETEKKTTLYLAIDNVKNLVAGGRVSKFAGTLSGVLNIKPMLKVGNGEIEIPAKVRGNKGMHKLWSQIVADMVKGPEIVAIGISHVAAGKEMEWLKAQLNEAFPDIPVLVRTTVPVIATNTGFGANCLIYYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHU6</accession>
    <name>H4GHU6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_11330</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RsuA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121630.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11330"/>
    <dbReference type="EnsemblBacteria" id="EHS87502">
        <property type="protein sequence ID" value="EHS87502"/>
        <property type="gene ID" value="PS3_11330"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.67"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042092">
        <property type="entry name" value="PsdUridine_s_RsuA/RluB/E/F_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000748">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB/E/F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018496">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00093">
        <property type="entry name" value="TIGR00093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01149">
        <property type="entry name" value="PSI_RSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="4">
        <location>
            <begin position="2"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="239" mass="26895" checksum="8FBADDDE054FB32F" modified="2012-04-18" version="1">MERLQKVMAQAGVASRRASEKLITEGHVEVNGQLVTELGTKVSVHDLVSVDGVPIEPEPTEYYILNKPRQVVSTAHDDKGRRTVVDILYDDGVETRVYPVGRLDYDTTGLLVLTNDGGLAQSLMHPKYEIDKVYIAKIKGKLSREQEKQLRDGVKFDGHRARGAKVKVISTDDSRHTQIVELTIHEGHYHQVKKMLQAVGHPVMKLHRESYGPLTLGNLQAGEWRPLKPHEIHELKQAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHV6</accession>
    <name>H4GHV6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Potassium transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_9398</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121640.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9398"/>
    <dbReference type="EnsemblBacteria" id="EHS87512">
        <property type="protein sequence ID" value="EHS87512"/>
        <property type="gene ID" value="PS3_9398"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.81"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="1">
        <location>
            <begin position="6"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="2">
        <location>
            <begin position="137"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="223" mass="24793" checksum="095E66A82FCCD873" modified="2012-04-18" version="1">MAKKESYVVIGLGKFGMTICSALAQAGQEVLAIDSNPDVVNEVSHLVVRAVIANAEEEETLKELAVGNFDHVYISIGHNIEASIMATLIVKELGAPDVTCRAENIHHARVLERVGADTVVRPEHDIAERLVFRKLNPNVVDFVRISENLMLAEVTIRNPKFFNRSLGELEIRRRFNVNVIAIVDHQDQLNEMPMATDTIHDHDKLTVIGSQAAIEQLSDEVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHW5</accession>
    <name>H4GHW5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Flavoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_13247</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121649.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13247"/>
    <dbReference type="EnsemblBacteria" id="EHS87521">
        <property type="protein sequence ID" value="EHS87521"/>
        <property type="gene ID" value="PS3_13247"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.91"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="2">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="375"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="475" mass="52205" checksum="F06143426F868B99" modified="2012-04-18" version="1">MKLVAIAGSIAKDSYNKKLVNFMAKAYSDVANIEVLSLDQVPLFDRDLSYKDFDQLVELDRKIKLADGVILATPEHNHTTTVAMKSMLEWLSTDTHPFQDKPVLIVGASYHIQGTSRAQMDLRDILDAPGVNAIALPGNDFLLGNCKEKFDEQGNLNDEGTIKFLNSVLVKFTEWVNTINAVSAEEAADWTKEDLTASGITDTTIDVDMMSKDWVNQAAAKTHAAEDTDYVKLDSGVLSVNQLNWFLKTMPMELTYMDDNDQFIYYNHYLDRDDMLAPRYPWQTGMPMSEVHPQRAMNGVKRVINQLRYNKTDLVKMPVPGNKVNEKWLMHYYKRMADEEGKFRGINEWVLDIWPIVAQYLEMTGQKLVADPNAKVDAHGGASGKPASPSVEANQTDTDAGASDSDSEETSAPVAEPAVDTNAGASGEEEETTTSASPVAKVEPSVDTDAGASSEEDDAANGIPDSVDTDAGASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHW8</accession>
    <name>H4GHW8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase sigma factor SigA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">sigA</name>
        <name evidence="10" type="ORF">PS3_5701</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts transiently with the RNA polymerase catalytic core.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the sigma-70 factor family. RpoD/SigA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121652.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5701"/>
    <dbReference type="EnsemblBacteria" id="EHS87524">
        <property type="protein sequence ID" value="EHS87524"/>
        <property type="gene ID" value="PS3_5701"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.94"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00963">
        <property type="entry name" value="Sigma70_RpoD_SigA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009042">
        <property type="entry name" value="RNA_pol_sigma70_r1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007127">
        <property type="entry name" value="RNA_pol_sigma_70_r1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042189">
        <property type="entry name" value="RNA_pol_sigma_70_r1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012760">
        <property type="entry name" value="RNA_pol_sigma_RpoD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028630">
        <property type="entry name" value="Sigma70_RpoD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03979">
        <property type="entry name" value="Sigma70_r1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00140">
        <property type="entry name" value="Sigma70_r1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02393">
        <property type="entry name" value="RpoD_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00716">
        <property type="entry name" value="SIGMA70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RNA_pol_sigma70" evidence="8">
        <location>
            <begin position="170"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_sigma70" evidence="9">
        <location>
            <begin position="339"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="6">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-2" evidence="6">
        <location>
            <begin position="146"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-3" evidence="6">
        <location>
            <begin position="225"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-4" evidence="6">
        <location>
            <begin position="314"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Interaction with polymerase core subunit RpoC" evidence="6">
        <location>
            <begin position="170"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="379" mass="43102" checksum="5B87C6FF354C6989" modified="2012-04-18" version="1">MAKTNKDLVISNAVEFDQSAYDKAVGKLIREHKQAKEIKYSELSTQLAEPFELNADGIDQLMQNVEDSGISIVDENGDPDPRALQATEKLSNSASKDTSAPSNVKINDPVRMYLKEIGRVPLLTADQEVELALRIEQGDEVAKQELAEANLRLVVSIAKRYVGRGMQFLDLIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRIQRQLLQDLGREPLPEEIGAEMDMETQKVRDILKIAQEPVSLETPIGEEDDSHLGDFIEDQDATSPAEHAAYEMMKKQLENVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHY8</accession>
    <name>H4GHY8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">tRNA/rRNA methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_10007</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000006">
        <property type="protein sequence ID" value="EHS87467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121672.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000006.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10007"/>
    <dbReference type="EnsemblBacteria" id="EHS87467">
        <property type="protein sequence ID" value="EHS87467"/>
        <property type="gene ID" value="PS3_10007"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.116"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="Fmu/NOL1/Nop2p_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031341">
        <property type="entry name" value="Methyltr_RsmF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027391">
        <property type="entry name" value="Nol1_Nop2_Fmu_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031340">
        <property type="entry name" value="RsmF_methylt_CI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17125">
        <property type="entry name" value="Methyltr_RsmF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13636">
        <property type="entry name" value="Methyltranf_PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17126">
        <property type="entry name" value="RsmF_methylt_CI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="7">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="5">
        <location>
            <begin position="104"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="450" mass="49937" checksum="AB8BD2A189CBBE24" modified="2012-04-18" version="1">MELPTAFIEKYQRLLGDEAADFLTALQAPSQAGFRSNPLKPALPTATIAQAKGKVAAVPTGYYGQVDGHSLDHVTGWVYSQEPSAMLVGEVVAPQAGEKVLDLCAAPGGKSTHLIGKMQDQGLLVANEIFPKRAKILAENLERWGANQAVVTSESPDHLAPQFPQFFDRILVDAPCSGEGMFRKEPEGIQYWHPDYPLECANRQRKILGSALQMLKPGGTLVYSTCTFAPEEDEQVIAWLLDTYSELSLVEIPKQAGMDAGRPAWANGNPELTKTLRLFPHHYQGEGHFIAKLHRAGEPHASKRAKPRKGQRPSLTKEQQQYWEEFRQSVLPAYQPERLVVFGDHLIAMPDLFPRDAKVKIIQGGVELGIFKKKRFEPSHALALACQQARQVEISLDDWRSYVHGDVITLTDPPTNGWYVVTCQDHHCGWGKVTGSNLKNFFPKGLRFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHZ2</accession>
    <name>H4GHZ2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoenolpyruvate-dependent sugar phosphotransferase system EIIA.1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_10160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000006">
        <property type="protein sequence ID" value="EHS87471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121676.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000006.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10160"/>
    <dbReference type="EnsemblBacteria" id="EHS87471">
        <property type="protein sequence ID" value="EHS87471"/>
        <property type="gene ID" value="PS3_10160"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.121"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00211">
        <property type="entry name" value="PTS_IIA_fru"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002178">
        <property type="entry name" value="PTS_EIIA_type-2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00359">
        <property type="entry name" value="PTS_EIIA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="SSF55804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51094">
        <property type="entry name" value="PTS_EIIA_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PTS EIIA type-2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="150" mass="17027" checksum="5F9E39471278E402" modified="2012-04-18" version="1">MLFDKQIILFDQEMADQEMALTTLASQLEKAGLVTSDFTTAIIQREHQFPTGLLTQTMGVAIPHTDADKVIEPQIAFMRLKKPVIFHQMGDNQEIAVQIIFMLALKQGQDQLKMLQKLMALFQDSSAMHHLMTIRSNEELITLMQVAQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI01</accession>
    <name>H4GI01_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">33 kDa chaperonin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Heat shock protein 33 homolog</fullName>
            <shortName evidence="6">HSP33</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hslO</name>
        <name evidence="7" type="ORF">PS3_987</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Redox regulated molecular chaperone. Protects both thermally unfolding and oxidatively damaged proteins from irreversible aggregation. Plays an important role in the bacterial defense system toward oxidative stress.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">Under oxidizing conditions two disulfide bonds are formed involving the reactive cysteines. Under reducing conditions zinc is bound to the reactive cysteines and the protein is inactive.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the HSP33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000007">
        <property type="protein sequence ID" value="EHS87452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121685.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000007.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_987"/>
    <dbReference type="EnsemblBacteria" id="EHS87452">
        <property type="protein sequence ID" value="EHS87452"/>
        <property type="gene ID" value="PS3_987"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.131"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00498">
        <property type="entry name" value="Hsp33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00117">
        <property type="entry name" value="HslO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000397">
        <property type="entry name" value="Heat_shock_Hsp33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016154">
        <property type="entry name" value="Heat_shock_Hsp33_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016153">
        <property type="entry name" value="Heat_shock_Hsp33_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30111">
        <property type="entry name" value="PTHR30111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01430">
        <property type="entry name" value="HSP33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005261">
        <property type="entry name" value="Heat_shock_Hsp33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118352">
        <property type="entry name" value="SSF118352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64397">
        <property type="entry name" value="SSF64397"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0862">Zinc</keyword>
    <feature type="disulfide bond" description="Redox-active" evidence="6">
        <location>
            <begin position="239"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="6">
        <location>
            <begin position="272"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="295" mass="31936" checksum="DA65119171CC3DB1" modified="2012-04-18" version="1">MTDYLVKGITNNGMFRIYTLNATATVEAARQIHDTWSGASAALGRTMIGTILLSTSGLQGEGRLTVKIQGDGPVGYMVADGTAQGTVKAYIQNPHVNLPLNDQHKIDVRGAVGTNGTLSVTKMTPGDKTPYTGEVNLVSGELGEDFTYYLAQSEQIPSAVGLSVFVESDDSIGVAGGFLVQVLPGASDEEIGKLETTIKDLPLVSEMLREGETPETIMERIFPANELKVLEKLPVKYECDCSKKRFAQALVAIKKADLKQLIEEDHGAEAVCRFCHKKYHFNEVELTALLDQKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI11</accession>
    <name>H4GI11_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase</fullName>
            <ecNumber evidence="7 8">6.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">D-alanyl-D-alanine-adding enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">murF</name>
        <name evidence="12" type="ORF">PS3_9146</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + D-alanyl-D-alanine + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-L-lysine = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine</text>
            <dbReference type="Rhea" id="RHEA:16085"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57822"/>
            <dbReference type="ChEBI" id="CHEBI:70758"/>
            <dbReference type="ChEBI" id="CHEBI:83903"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + D-alanyl-D-alanine + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine</text>
            <dbReference type="Rhea" id="RHEA:28374"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57822"/>
            <dbReference type="ChEBI" id="CHEBI:61386"/>
            <dbReference type="ChEBI" id="CHEBI:83905"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7 8">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MurCDEF family. MurF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.10" evidence="7 8"/>
    <dbReference type="EMBL" id="AICN01000007">
        <property type="protein sequence ID" value="EHS87462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121695.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000007.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9146"/>
    <dbReference type="EnsemblBacteria" id="EHS87462">
        <property type="protein sequence ID" value="EHS87462"/>
        <property type="gene ID" value="PS3_9146"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.145"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047480">
        <property type="term" value="F:UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008766">
        <property type="term" value="F:UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02019">
        <property type="entry name" value="MurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035911">
        <property type="entry name" value="MurE/MurF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005863">
        <property type="entry name" value="UDP-N-AcMur_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63418">
        <property type="entry name" value="SSF63418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01143">
        <property type="entry name" value="murF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 7 8" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 7 8" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 7 8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase" evidence="9">
        <location>
            <begin position="26"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="11">
        <location>
            <begin position="110"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="10">
        <location>
            <begin position="317"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="112"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="460" mass="50379" checksum="0F493A9A7AB9AA77" modified="2012-04-18" version="1">MKMKLAEIAKAIDAQDINAAWDELEVTSVAFDSRNLTPGSLFVPLQGARDGHEFVAQAFQNGAKATLWAADHDIPSDDQFPRLVVKDPLAAFQRLSQYYLKKINPIVVAVTGSNGKTTTKDMAAAILATQFNVTKSFANFNNEIGVPVTLLNMEPSTEVVVVEMGMDRFDQLDLLSRLATPDFAVITMIGEAHIEFFGTRDRIADAKMEIVHGMNEDGTLIINGDEPLLTARAADIEQQVVTFGRQLADDFYATSVQLADDQVAFSVNEWPDLTFTLPMVGEYNVNNALAAIAVGAQLQITPENMQKALQGLQMTENRAEWIQGTNGERILSDVYNSNPTAVAEVLKTMEQVQAEGKKVIVLGDMLELGAASAQLHAQLAAEIDPDQIAEVYLVGNEMQALLKQLTTDGFPNQRLHHYDTNELAQLTKDLNATLRTDDIVLLKGSHGIHLETVLQGLTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI31</accession>
    <name>H4GI31_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">D-alanyl carrier protein</fullName>
            <shortName evidence="3">DCP</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">D-alanine--poly(phosphoribitol) ligase subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">dltC</name>
        <name evidence="6" type="ORF">PS3_18181</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Carrier protein involved in the D-alanylation of lipoteichoic acid (LTA). The loading of thioester-linked D-alanine onto DltC is catalyzed by D-alanine--D-alanyl carrier protein ligase DltA. The DltC-carried D-alanyl group is further transferred to cell membrane phosphatidylglycerol (PG) by forming an ester bond, probably catalyzed by DltD. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cell wall biogenesis; lipoteichoic acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="3">4'-phosphopantetheine is transferred from CoA to a specific serine of apo-DCP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DltC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000011">
        <property type="protein sequence ID" value="EHS87441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18181"/>
    <dbReference type="EnsemblBacteria" id="EHS87441">
        <property type="protein sequence ID" value="EHS87441"/>
        <property type="gene ID" value="PS3_18181"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.171"/>
    <dbReference type="UniPathway" id="UPA00556"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036370">
        <property type="term" value="F:D-alanyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070395">
        <property type="term" value="P:lipoteichoic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00565">
        <property type="entry name" value="DltC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003230">
        <property type="entry name" value="DltC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01688">
        <property type="entry name" value="dltC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="5">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="3 4">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="78" mass="8868" checksum="3935C4AC655C6919" modified="2012-04-18" version="1">MMNEKIQELLAAAAGRDVADFADHDENIFTSGKIDSMSTVQFLFSLQEEYGIQVPVSEFDRDQWSTINLIADRVKELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI40</accession>
    <name>H4GI40_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rhodanese domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_4600</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121724.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4600"/>
    <dbReference type="EnsemblBacteria" id="EHS87379">
        <property type="protein sequence ID" value="EHS87379"/>
        <property type="gene ID" value="PS3_4600"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.183"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="45"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="133" mass="15435" checksum="D3A7D45D4315666F" modified="2012-04-18" version="1">MNSFLLVFDLVVTVLLVFWLVTYVIGRYRRSHYATVLSQDEFQKGSHKAQVIDLRQKADFDRGHILGARNLPYAFMKQQYGELRSDLPIYMYDQGMSMSTNAAAFLGRRGYKKLYILDGGYAKWDGKTKKAKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI45</accession>
    <name>H4GI45_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_20418</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121729.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20418"/>
    <dbReference type="EnsemblBacteria" id="EHS87384">
        <property type="protein sequence ID" value="EHS87384"/>
        <property type="gene ID" value="PS3_20418"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.190"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025997">
        <property type="entry name" value="SBP_2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13407">
        <property type="entry name" value="Peripla_BP_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="5"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="334" mass="37490" checksum="96E5A4C646E01A71" modified="2012-04-18" version="1">MGKRATISDVARQAHVSATTVSRYLNHHYQKMSDETKARIAQVIADLDYSPSSSARQMRQKSSHLVGVLVGDIANPFSSLMAKGIYDVLQPAGYDIQLMNSNDSKEAESRHLDRLIEQRADGIIVQPSAASFAQFERCRTNGIPIIIVDRTMKDLPTDVAYVTSENFDACYRLGPILVDHGYQNVISVSSRLAEASGQILRIKGLMQTANDHQLQYINLEIRDGNVEWLKAELQQNLQELSGRTAVISLMGPLLFNLLDIFRGLNLTFPQDIGLISYDDWDWSRFVGDGIFLLRQDMELMGSQAAQFLLEMIHDPKKKLGTEYLPVTMVERPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI63</accession>
    <name>H4GI63_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal silencing factor RsfS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rsfS</name>
        <name evidence="3" type="ORF">PS3_6172</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Functions as a ribosomal silencing factor. Interacts with ribosomal protein L14 (rplN), blocking formation of intersubunit bridge B8. Prevents association of the 30S and 50S ribosomal subunits and the formation of functional ribosomes, thus repressing translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with ribosomal protein L14 (rplN).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Iojap/RsfS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121747.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6172"/>
    <dbReference type="EnsemblBacteria" id="EHS87402">
        <property type="protein sequence ID" value="EHS87402"/>
        <property type="gene ID" value="PS3_6172"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.216"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:mature ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090071">
        <property type="term" value="P:negative regulation of ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01477">
        <property type="entry name" value="Iojap_RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004394">
        <property type="entry name" value="Iojap/RsfS/C7orf30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21043">
        <property type="entry name" value="PTHR21043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00090">
        <property type="entry name" value="rsfS_iojap_ybeB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="118" mass="13324" checksum="1E5BEA0D751D3CF7" modified="2012-04-18" version="1">MNSEEILKMVVTEADGRRAEDIVALKVDQISPMADYFVVMTGGSNRQVQAISDAIVKKAHELKLPIGSVEGKNAAQWILVDLGEVVVHIFREETRQFYNLEKLWSEAPLVNINDWIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI70</accession>
    <name>H4GI70_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Translation initiation factor IF-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">infC</name>
        <name evidence="8" type="ORF">PS3_11884</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the IF-3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11884"/>
    <dbReference type="EnsemblBacteria" id="EHS87409">
        <property type="protein sequence ID" value="EHS87409"/>
        <property type="gene ID" value="PS3_11884"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.224"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00080">
        <property type="entry name" value="IF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036788">
        <property type="entry name" value="T_IF-3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036787">
        <property type="entry name" value="T_IF-3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019813">
        <property type="entry name" value="Translation_initiation_fac3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001288">
        <property type="entry name" value="Translation_initiation_fac_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019815">
        <property type="entry name" value="Translation_initiation_fac_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019814">
        <property type="entry name" value="Translation_initiation_fac_3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10938">
        <property type="entry name" value="PTHR10938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00707">
        <property type="entry name" value="IF3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05198">
        <property type="entry name" value="IF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54364">
        <property type="entry name" value="SSF54364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55200">
        <property type="entry name" value="SSF55200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00168">
        <property type="entry name" value="infC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00938">
        <property type="entry name" value="IF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF3_N" evidence="7">
        <location>
            <begin position="3"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="IF3_C" evidence="6">
        <location>
            <begin position="80"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="170" mass="19602" checksum="52FF393028A795B6" modified="2012-04-18" version="1">MFVNDGIRAREVRLIDQNGEQLGVKTKAEALQLAEAADLDLVLVAPKARPAVARIMDYGKYRFEMQKKERENRKNQKTTAVKEIRLSPTIDTNDFNVKLSRVRKFIEKGDKVRVSLRFRGRAITHKDIGRDMIERMAEETKDIATVEQRPKMEGRSMFLTLAPLSDKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI73</accession>
    <name>H4GI73_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcriptional repressor NrdR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nrdR</name>
        <name evidence="10" type="ORF">PS3_5389</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR-boxes.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NrdR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121757.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5389"/>
    <dbReference type="EnsemblBacteria" id="EHS87412">
        <property type="protein sequence ID" value="EHS87412"/>
        <property type="gene ID" value="PS3_5389"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.227"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00440">
        <property type="entry name" value="NrdR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003796">
        <property type="entry name" value="RNR_NrdR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30455">
        <property type="entry name" value="PTHR30455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00244">
        <property type="entry name" value="TIGR00244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ATP-cone" evidence="9">
        <location>
            <begin position="49"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="zinc finger region" evidence="7">
        <location>
            <begin position="3"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="150" mass="17437" checksum="AD134345D55191BB" modified="2012-04-18" version="1">MICPHCRQEGSRVIDSRPADDGSSIRRRRECPNCGFRFTTFERYEEIPLLVIKKDGSREPFNRQKLLNGLIRSAEKRSVGMQTLTEIADKVESQARRLGETEISTQAIGEFVMNELKPVDEIAYIRFASVYREFKDVEAFRKEIESMEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI78</accession>
    <name>H4GI78_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">EMAP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_14631</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121762.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14631"/>
    <dbReference type="EnsemblBacteria" id="EHS87417">
        <property type="protein sequence ID" value="EHS87417"/>
        <property type="gene ID" value="PS3_14631"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.233"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02796">
        <property type="entry name" value="tRNA_bind_bactPheRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027855">
        <property type="entry name" value="DUF4479"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033714">
        <property type="entry name" value="tRNA_bind_bactPheRS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037154">
        <property type="entry name" value="YtpR-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14794">
        <property type="entry name" value="DUF4479"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="4">
        <location>
            <begin position="93"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="213" mass="22498" checksum="96E9CD6D975EE924" modified="2012-04-18" version="1">MLISSYNQAGMGDVLVTMVAPTVGGQTVHKAGQVVQLTSSENGSLLGYNFLAASELLPELTSQAAVQLFLTDGQVAKLNQAVQAAGFDQLLVADHDPKFVIGYVAEMEDHPKSDHLHITKVDVGDQQLQIVCGSPNIANHVKVIVAKPGAMMPSGQMIWPGELVGVPSFGMICAGRELNLANAPQQPGCVILPDDFGQAGDAFDFEKGNQLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI94</accession>
    <name>H4GI94_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglycerate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_5994</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121778.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5994"/>
    <dbReference type="EnsemblBacteria" id="EHS87315">
        <property type="protein sequence ID" value="EHS87315"/>
        <property type="gene ID" value="PS3_5994"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.258"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="217" mass="24785" checksum="CDDA6B35403165F4" modified="2012-04-18" version="1">MTKLNIFLVRHGQTYFNIYNKLQGWSNSPLTQQGREDAQRVGQRLKDIHLDAAFCSDLTRAVETCQTILNVNEADSVVRPRTTPDLREEFYGVFEGTNMSLAWEMAGLPHGYKTFKDIVTDYSINQAKDWLKEADPFHDAENSQEYWERLDRGLATIRQADLLDEANVLLVSHGNTCLSLIERFGEGKYDITERPANGSLSKLVMTDDDVKVITYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI96</accession>
    <name>H4GI96_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_7286</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7286"/>
    <dbReference type="EnsemblBacteria" id="EHS87317">
        <property type="protein sequence ID" value="EHS87317"/>
        <property type="gene ID" value="PS3_7286"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.261"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003352">
        <property type="entry name" value="PTS_EIIC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13303">
        <property type="entry name" value="PTS_EIIC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="PTS_EIIC" evidence="9">
        <location>
            <begin position="29"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="369" mass="38154" checksum="476E31C352E842BA" modified="2012-04-18" version="1">MNNSTAHEHKGAKKTYANSSKWMEAAYRIFAAVANAVLVILGGGLLVQTIGQLTGIQALTLVGAAIQVLLAPAIGYAVASQVHTNTLVTFASMASAMIGANAVHFTTTATQALTATGQAYATAANAPVYTTGQPISALLAAVCATIVGKWLTGKTPLDMVLVPFGALAVGVVAGLALAAVVTPALLAVSKWIANMEQVSPVIGSIAISVVWALFLMTPASSAALSVALMLDPISSAAALIGTTSQFVNYTVMSFRQNNAGANIAQFVVTPKIQFPNILINPWLLVPSVTAAAICAPIATSLGFRSTYTLGGLGLNSFIAPIAYLSKGPYQLMLYVLFGMIVPAVISLALYFFMKKIGLIGENQLHLDIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIB6</accession>
    <name>H4GIB6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative sugar-specific permease, SgaT/UlaA family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_17377</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121800.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17377"/>
    <dbReference type="EnsemblBacteria" id="EHS87337">
        <property type="protein sequence ID" value="EHS87337"/>
        <property type="gene ID" value="PS3_17377"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.287"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004703">
        <property type="entry name" value="PTS_sugar-sp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03611">
        <property type="entry name" value="EIIC-GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="401"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="445"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="504" mass="56020" checksum="6E5D1A0DA979633A" modified="2012-04-18" version="1">MDAVLNFLMACWEYFATNILEQPAFMIGFIVLLGYLLERKPWYECVSGFLKATVGYLILNVGSAGLVNSFRPILVGLRDRFNLSAMVTDPYFGQNAVTAGIYKTFGRTFADTMLLLLFAYIFNILLVRFQKWTKLRSVFTTGNVQVQQAATAFWLFLFCFPKMREIPILIVMSIILGLYWAVGSNLTVGITQELTDGAGFAIAHQQMFGIFFFAKLAEMWGKSNKKKGRKSGRRLEDIELPGFMSMFNDNMVSTSILMIVFFGIILIVLGKPYLVHAKFMTADQSFLFYLLTTSLNFAVYLAILQLGVRTFVAELTESFQGISDRWLPGSVPGIDVAATLGFGDASNAATIGFMFGALGQFVMIILLLVFHSPTIVIAGFIPMFFDNAAIAVYANNRGGVVAAMLFPFLCGLIQISGSALIATWVGMAQYGGYIGMFDWATVWPFFTVLMKYMGYIGIALVIIILVAIPQLQYRADPKGYFQVVTDYEAWKASHHDEAEGAAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GID2</accession>
    <name>H4GID2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dipeptidase</fullName>
            <ecNumber evidence="5">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_3338</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an L-aminoacyl-L-amino acid + H2O = 2 an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:48940"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="ChEBI" id="CHEBI:77460"/>
            <dbReference type="EC" id="3.4.13.19"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the peptidase C69 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121816.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="MEROPS" id="C69.001"/>
    <dbReference type="EnsemblBacteria" id="EHS87353">
        <property type="protein sequence ID" value="EHS87353"/>
        <property type="gene ID" value="PS3_3338"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.313"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070004">
        <property type="term" value="F:cysteine-type exopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016805">
        <property type="term" value="F:dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005322">
        <property type="entry name" value="Peptidase_C69"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12994">
        <property type="entry name" value="PTHR12994"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03577">
        <property type="entry name" value="Peptidase_C69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="477" mass="54338" checksum="22F11AED61F7E808" modified="2012-04-18" version="1">MSQSLSACTTVLVGKKATIDGSTMAARNDDTFKPLTPQRVVVYPKYRQHPNQVHSYLNHFVGDRPAEGARYQGVPNVNYEQEGVFDENGFNEFNVGTSATESVYANEHVLAFDPLNTESGINEDVIVAMTLPFIHSAKEGVQYLGQLIAKYGSAEGNGVIFVDRDDVWYMEIVTGHHWVAQRIPDQAYAVTGNRVAIQDVDFTDPHQFMWSDGIRDFVDQHHLNPDLKGWNFRHIFGTATTFDQHYNTPRQWYGHRLLKPSLELEPTDFDLPFIQEPDRKLSLTDVEAVLSSHYQNTPYDPLGHGSDADKQRFRPISLNRTQNSHILQVRSDLPEMASTIMWMSLGIPSFTPYVPFFGNADTLDATYRLTPPEMNPDFKSAYWMYRGLSMLVESHYAEFIQADYDYLKDAREYLYRFVEDTAHQTADLTANEAIQVLNTASTELTAEMAQRTKKLMSQLMMHGLELSKLTFKMDANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GID3</accession>
    <name>H4GID3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ribonucleotide reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_4921</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121817.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4921"/>
    <dbReference type="EnsemblBacteria" id="EHS87354">
        <property type="protein sequence ID" value="EHS87354"/>
        <property type="gene ID" value="PS3_4921"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.316"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004465">
        <property type="entry name" value="RNR_NrdI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37297">
        <property type="entry name" value="PTHR37297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07972">
        <property type="entry name" value="Flavodoxin_NdrI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005087">
        <property type="entry name" value="NrdI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="157" mass="18030" checksum="F6489646AB552F35" modified="2012-04-18" version="1">MDPLYCLYISISGNTRSFMQRLKEYALQLHTINPDQDYPLIDLKEISDQSIPAREDRPFFVFVPTYLDGGNGFETGTTEVMTNSFGEYLLDGDNTDLVMGVVGSGNKNFNEQYCLTARKYAKECHSEVIGDYELRGTDEDIERIYHQLVQVAKNYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIF5</accession>
    <name>H4GIF5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription elongation factor GreA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Transcript cleavage factor GreA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">greA</name>
        <name evidence="7" type="ORF">PS3_5345</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GreA/GreB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121839.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5345"/>
    <dbReference type="EnsemblBacteria" id="EHS87376">
        <property type="protein sequence ID" value="EHS87376"/>
        <property type="gene ID" value="PS3_5345"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.352"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00105">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036953">
        <property type="entry name" value="GreA/GreB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028624">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001437">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023459">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022691">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036805">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30437">
        <property type="entry name" value="PTHR30437"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01272">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03449">
        <property type="entry name" value="GreA_GreB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006092">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46557">
        <property type="entry name" value="SSF46557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="GreA_GreB_N" evidence="6">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="GreA_GreB" evidence="5">
        <location>
            <begin position="83"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="157" mass="17801" checksum="D28D4AD9FF4C6300" modified="2012-04-18" version="1">MPEIYYQLMTITGYQEIESQITKLKSQRPTLVARLKAARALGDLSENTGYSTAKRDLRHIDSRLRFLNKQLKYAKVIEPTDSKLVEIGTKVVLEDLSDQEEMIYQVVGRQEADVNQGKISYESPLGSAILHQSVGKIVTVTAPEFTYQVRIIKIELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIF7</accession>
    <name>H4GIF7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Haloacid dehalogenase (HAD) superfamily hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_2747</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000015">
        <property type="protein sequence ID" value="EHS87306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121841.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000015.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2747"/>
    <dbReference type="EnsemblBacteria" id="EHS87306">
        <property type="protein sequence ID" value="EHS87306"/>
        <property type="gene ID" value="PS3_2747"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.354"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000150">
        <property type="entry name" value="Cof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00099">
        <property type="entry name" value="Cof-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="273" mass="30273" checksum="CBD94FD5ABAC363F" modified="2012-04-18" version="1">MQQMIALDLDNTLFNSDKEISPRNEQVLKRVHQAGVKIVLCTGRPINAIRNYLDQLELNQAEDYTINFNGGLVTNNQTGEFLFQKGLVKSDLDVLYEFVMAHQLPLDVLDFDKVYEIKTGQQSLYGATVKGIDFTNDDYQDLPAGDKLYSKAVIAVDPAQMAQVKALVRDDDQIGQHFTVVQSQPHILEFIPLGLNKAVGLQHLLEHFEMTFADLTAFGDADNDKEMLAAAGDGVVMANGLPAVKTIADHETLSNDEDGVAVYIENKFVTLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIH7</accession>
    <name>H4GIH7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Signal peptidase I</fullName>
            <ecNumber evidence="4 6">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_4766</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <topology evidence="6">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the peptidase S26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="4 6"/>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121861.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000017.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4766"/>
    <dbReference type="EnsemblBacteria" id="EHS87298">
        <property type="protein sequence ID" value="EHS87298"/>
        <property type="gene ID" value="PS3_4766"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.378"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019758">
        <property type="entry name" value="Pept_S26A_signal_pept_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43390">
        <property type="entry name" value="PTHR43390"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00761">
        <property type="entry name" value="SPASE_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S26" evidence="7">
        <location>
            <begin position="9"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="201" mass="22643" checksum="13D491FD4A4C974B" modified="2012-04-18" version="1">MKTFRSIMSWVIPVALGLAVALILNVFVVQRVKVDGPSMQPNLQNNEKVWTFKQAKIHRGSVIVFDANGVDPQVTSKTNYVKRVIAVGGDTVESKNGNLYVNGKKVSQSYISSYQRTTGTGNWTLQSISIKNGWLKYNGATKVPKGYYFVLGDHRSVSNDSRYWGFVPKSKILGVVKVAPWSGTKTTRYNINQFWKHYFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GII0</accession>
    <name>H4GII0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Threonine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Threonyl-tRNA synthetase</fullName>
            <shortName evidence="11">ThrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">thrS</name>
        <name evidence="13" type="ORF">PS3_14227</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + H(+) + L-threonyl-tRNA(Thr)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="11"/>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121864.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000017.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14227"/>
    <dbReference type="EnsemblBacteria" id="EHS87301">
        <property type="protein sequence ID" value="EHS87301"/>
        <property type="gene ID" value="PS3_14227"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.381"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="PTHR11451"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="12">
        <location>
            <begin position="228"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS87301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="601" mass="68661" checksum="DABD4E0E7BB6F6EA" modified="2012-04-18" version="1">MPQVAVMSPNGEVEKIDRDSQAGLEALRALSALLLKAALHQQFDGIRLGAAVAEDDQFYVDSDKDEQQVSADELASLEATIKQFAKDDVKVEFAQVKVEDALAEVKDDPFSTELINEHAQDGLVAMYEVAGVKSVATQPVLVYGNVVKNYQLLSVAGAYWKGMSSNPMLQRIYGTVFFKKDDLEADLKARQEARERDHRVIGNQLDLFFVDPKVGAGLPYWMPKGATIRRVIERYIIDKEVADDYEHVYTPVLMNLDAYKTSGHWDHYREDMFPPMDMGDGELLELRPMNCPSHIQVYKHHIRSYRDLPLRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVTLDQIQDEFAKILKLIMDVYKDFDITDYTFRLSYRDPKNTEKYFANDEMWEQAQKMLKGAMDDLGLDYYEAEGEAAFYGPKLDIQTKTALGNEETMSTIQLDFMLPEKFDLHYVGEDGQQHRPVMIHRGVVGTMERFMAYLTEIYKGAFPTWLAPQQVNIIPVSDDRHGEYADQVAKELKHLNIRAKVDHRNEKMGYKIRESQTQKVPYTLVVGDDEMANGAVSVRKYGEQEQNGMSKDDFLKLIQADIASYSREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIK8</accession>
    <name>H4GIK8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acetyl-CoA carboxytransferase</fullName>
            <ecNumber evidence="3">2.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PS3_10556</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + N(6)-carboxybiotinyl-L-lysyl-[protein] = malonyl-CoA + N(6)-biotinyl-L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:54728"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="EC" id="2.1.3.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.15" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10556"/>
    <dbReference type="EnsemblBacteria" id="EHS87203">
        <property type="protein sequence ID" value="EHS87203"/>
        <property type="gene ID" value="PS3_10556"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.419"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009317">
        <property type="term" value="C:acetyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001095">
        <property type="entry name" value="Acetyl_CoA_COase_a_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42853">
        <property type="entry name" value="PTHR42853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03255">
        <property type="entry name" value="ACCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01069">
        <property type="entry name" value="ACCCTRFRASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="257" mass="27908" checksum="ED60AFD0F9D9F9E6" modified="2012-04-18" version="1">MSQLTAAEIVKASRSESKITGVEIIQQLFPDFMELHGDRRGSDDPAIVGGLATFNGQPITVITTDRGRSIAERVAKHFGSPEPGGYRKALRLTEQAGKFHRPVVMLVNTAGAYPGRSAEENGQGQAIAENLLAISQIPTPIITIIYGEGGSGGALALACGDQVWMLANSTYSILSPEGFASILWKDSSRADEAAEVMQMTPEALLKKQVIEGVIPESTDHQVTTAQIEAVLKRELPKLQELTPEELLSQRHQRYRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIL4</accession>
    <name>H4GIL4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
            <ecNumber evidence="5">1.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_18352</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121898.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18352"/>
    <dbReference type="EnsemblBacteria" id="EHS87209">
        <property type="protein sequence ID" value="EHS87209"/>
        <property type="gene ID" value="PS3_18352"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.425"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011284">
        <property type="entry name" value="3oxo_ACP_reduc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01830">
        <property type="entry name" value="3oxo_ACP_reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="12"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="153"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR611284-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR611284-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU366074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="243" mass="25897" checksum="015A954D08245257" modified="2012-04-18" version="1">MELKDKVVLITGSSRGIGAATALAFAKAGSKVILNARHELPSELTDELTALNTEYTFLQADVADPDTIKDFAQTAWDAYGHVDVLVNNAGITRDKLMIGMKLDDFDQVINVNLRGPFMLTQAILKKMNKQRSGSIVNLASVVGLHGNAGQANYAASKGGIIGLTKTVAQEGAMRGIRCNAIAPGMIESDMTAVLSDKVKEQLLNQIPLKRLGNPEEVAQAALFLAQNDYVTGQVIVVDGGMTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIM0</accession>
    <name>H4GIM0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA N6-adenosine threonylcarbamoyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">N6-L-threonylcarbamoyladenine synthase</fullName>
            <shortName evidence="6">t(6)A synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA threonylcarbamoyladenosine biosynthesis protein TsaD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tsaD</name>
        <name evidence="8" type="ORF">PS3_9162</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121904.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9162"/>
    <dbReference type="EnsemblBacteria" id="EHS87215">
        <property type="protein sequence ID" value="EHS87215"/>
        <property type="gene ID" value="PS3_9162"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.433"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01445">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017860">
        <property type="entry name" value="Peptidase_M22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022450">
        <property type="entry name" value="TsaD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF6">
        <property type="entry name" value="PTHR11735:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03723">
        <property type="entry name" value="T6A_TsaD_YgjD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01016">
        <property type="entry name" value="GLYCOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TsaD" evidence="7">
        <location>
            <begin position="27"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="137"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="337" mass="36438" checksum="ED0491C65E115BA1" modified="2012-04-18" version="1">MAKQELILAFESSCDETSVAVVENGHKILSNIVATQIASHQRFGGVVPEVASRHHIEQITACINDALLEAQVTYDDLTAVAVTYGPGLVGSLLIGVTAAKAVAWAHHLPLVPVNHMAGHLYAARFEHEFSYPQMGLLVSGGHTELVYMAAPHEYQIIGETRDDAAGEAYDKIGRVMGIAYPAGKRVDEMALQGTDTFHFPRAMEKEDNLDFSFSGLKSAFINTVHNAQQRGKQLDDNDLAASFQASVVDVLVEKTIKALDQYPVKQLILAGGVAANRGLRSRLEKDLVKFHPEVDLLKAPLKLCGDNAAMIGAAAHIGYQYGDRADWSLNAQPGLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIM7</accession>
    <name>H4GIM7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">YaaQ protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_3353</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121911.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3353"/>
    <dbReference type="EnsemblBacteria" id="EHS87222">
        <property type="protein sequence ID" value="EHS87222"/>
        <property type="gene ID" value="PS3_3353"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.441"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010375">
        <property type="entry name" value="CdAMP_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38456">
        <property type="entry name" value="PTHR38456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06153">
        <property type="entry name" value="CdAMP_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="106" mass="11518" checksum="F8A61D6AA68C0222" modified="2012-04-18" version="1">MKMVIAIVQKKDSNHLQSALIEAGFRSTRLASVGGFLSEGNVTFLIGVVDPQIPMVMEIIREHAQSRDQFVSSPAATDLGRSTMPVNVQVGGATVFVLPVEQFEQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIR2</accession>
    <name>H4GIR2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="14">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Methionyl-tRNA synthetase</fullName>
            <shortName evidence="14">MetRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">metG</name>
        <name evidence="19" type="ORF">PS3_4962</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 14">Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="14"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4962"/>
    <dbReference type="EnsemblBacteria" id="EHS87257">
        <property type="protein sequence ID" value="EHS87257"/>
        <property type="gene ID" value="PS3_4962"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.482"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02800">
        <property type="entry name" value="tRNA_bind_EcMetRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01228">
        <property type="entry name" value="Met_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004495">
        <property type="entry name" value="Met-tRNA-synth_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023457">
        <property type="entry name" value="Met-tRNA_synth_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43326">
        <property type="entry name" value="PTHR43326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00399">
        <property type="entry name" value="metG_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 14 16" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="9 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 14 16" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="18">
        <location>
            <begin position="569"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="539"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="14">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="14">
        <location>
            <begin position="310"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="542"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EHS87257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="673" mass="75746" checksum="2C4C285784CBA5E7" modified="2012-04-18" version="1">MAENKPTYYITTPIYYPSGKLHIGNSYTTVACDAEARFRRMSGYDVFFLTGTDEHGLKIEQKAEKLGLDPQAYVDQMAAGIKELWKKLEITNDKFIRTTDDYHEAAVQKIFQQLLDQGDIYKGEYIGWYSVDDEEYFTESQLAEVYRDDQGQVIGGKAPSGHEVELVKEESYFFKMSKYADWLMDYYQAHPDFIQPSSRMNEMINNFLKPGLEDLAVTRTSFNWGVKVPNDPKHVVYVWIDALSNYITALGYGSDDDSLYQKYWPADVHMVGKEIVRFHTIYWPIMLHALGLPLPKHVIGHGWLTMKDGKMSKSKGNVIYPETLIERYGLDATRYYLLRAMPFGNDGIFSPEDFVDKVNYDLANDLGNLLNRTVAMVNKYQGGQLVATNDATTDFDADLKATFEEVLANYRTLMADTHFADALAEVWKFVSRANKYIDETEPWVLAKDESKAAVLSDVMTNLAKSLRVIAALIQPVMPNAPREILSQLGLAETTLTLNDLSFDDFPATAQVAAKGTPIFPRLDVPAEVKFIKSQMTANTKQKGRKAMEEAKQQAQEAESTGKKSIRIDAFDKVELKVAQIKAVDHVEGADKLLKFQLDDGTAEGRQILSGIAQWYPQPEILVGKKVVIVANLKPRKMRGELSQGMLLSAEHDGEVELVTLPDTDVMVPGAGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIR4</accession>
    <name>H4GIR4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_10204</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121948.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10204"/>
    <dbReference type="EnsemblBacteria" id="EHS87259">
        <property type="protein sequence ID" value="EHS87259"/>
        <property type="gene ID" value="PS3_10204"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.484"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="3">
        <location>
            <begin position="3"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="234" mass="26234" checksum="5A266FB77B4736E0" modified="2012-04-18" version="1">MANPHQALYDEAKAIVFLTGAGVSTASGIPDFRSANGLYRQNQRAEYYLSHQYFATDPAGFYDYCQQNLYFPDAKPNVIHQKQAALTQQDRATVITQNIDNLYELAGTKHLIDFHGNLYDLYCVKCHQKVSLADYQTSRIHSSDGGEIRPNIVLYDEPIDSQNVQKSVAAIQQADLVVIVGTSMRVYPFAGLLDYVNSQAKVIAVNQEPLQLPIDFEMVTVDASQYFKELKINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIT1</accession>
    <name>H4GIT1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_2992</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121965.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2992"/>
    <dbReference type="EnsemblBacteria" id="EHS87161">
        <property type="protein sequence ID" value="EHS87161"/>
        <property type="gene ID" value="PS3_2992"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.510"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="267" mass="30231" checksum="C14E351DBBDA443C" modified="2012-04-18" version="1">MNNKVLFGSYVPTNSWLHRLDPRAKIIACFWFVILVFFAKNFWTYAWLTFILGIAMVMTKISFKMYWNGIKVLIGLITFTVAIQILFSSGGTVYWQWGILAITSGGLKQALVIIMRFVLIINASTVLTLTTESLELATGLEILMKPLQALKVPVNQLTMMMSIALRFVPTLMNEVTTIMNAQRSRGVNFNEGSLIKRIKQLVPIMVPLLVSSFKRADELATAMEARGYNPDQARSQYRVIAWQRQDTQAMVAMLVVTIALGLLRIWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIU2</accession>
    <name>H4GIU2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">30S ribosomal protein S5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpsE</name>
        <name evidence="11" type="ORF">PS3_11349</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body.</text>
    </comment>
    <comment type="function">
        <text evidence="7">With S4 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 30S ribosomal subunit. Contacts proteins S4 and S8.</text>
    </comment>
    <comment type="domain">
        <text evidence="7">The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 9">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121975.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11349"/>
    <dbReference type="EnsemblBacteria" id="EHS87172">
        <property type="protein sequence ID" value="EHS87172"/>
        <property type="gene ID" value="PS3_11349"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.523"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01307_B">
        <property type="entry name" value="Ribosomal_S5_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_S5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005712">
        <property type="entry name" value="Ribosomal_S5_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_S5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_S5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_S5_N_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="PTHR13718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01021">
        <property type="entry name" value="rpsE_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S5 DRBM" evidence="10">
        <location>
            <begin position="15"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="170" mass="17666" checksum="8477BE8DEE4A457B" modified="2012-04-18" version="1">MASTQSYIDPAKLDLEDQVVSINRITKVVKGGRRLRFAALVVVGDHNGHVGFGTGKAQEVPEAIRKASAAAQKNLISVPMVGTTIPHEVIGTFGGGKIMLKPSAEGTGVTAGGAVRNVMELAGVADVTSKRLGSNTPINVVRATFEGLKALKSAEEVSQLRGVSVDHLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIW1</accession>
    <name>H4GIW1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0340 protein PS3_11359</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_11359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0340 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11359"/>
    <dbReference type="EnsemblBacteria" id="EHS87133">
        <property type="protein sequence ID" value="EHS87133"/>
        <property type="gene ID" value="PS3_11359"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.545"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00800">
        <property type="entry name" value="UPF0340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028345">
        <property type="entry name" value="Antibiotic_NAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006340">
        <property type="entry name" value="DUF436"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04260">
        <property type="entry name" value="DUF436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007510">
        <property type="entry name" value="UCP007510"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110710">
        <property type="entry name" value="SSF110710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01440">
        <property type="entry name" value="TIGR01440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="185" mass="19939" checksum="E3767B37C3EEE177" modified="2012-04-18" version="1">MTQINEALTKGLTEFLDRANYEPGDLFVLGCSTSEIQGQRIGQDSNLAVGETVISTVLPLVQSHGLHLAVQGCEHLNRALVIERAVAKQLAYEPVTVYPSLKAGGATQVAAFKAFDDPIMVEHVQAQLGMDVGDTAIGMQVKFVQIPVRTSVTEVGHAHTTYLASRPKLIGGERAVYTWSADNYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIX2</accession>
    <name>H4GIX2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MerR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_9984</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122005.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9984"/>
    <dbReference type="EnsemblBacteria" id="EHS87144">
        <property type="protein sequence ID" value="EHS87144"/>
        <property type="gene ID" value="PS3_9984"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.564"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="1">
        <location>
            <begin position="8"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="144" mass="16548" checksum="7D6C2097C8DDE2AB" modified="2012-04-18" version="1">MDTIANETYSIGEVAAMFNLSVPTLRYYDHEGLILNLQKNAAGVRQFTEQNINAINIIECLKRAGMSIKNIKQFMKWCDQGDATLDLRLAMFQELKTQVQHQMDELQATMDTIDYKCHYYQQAVADGTEKYVAQSHHHLDTDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ25</accession>
    <name>H4GJ25_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_15781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122058.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15781"/>
    <dbReference type="EnsemblBacteria" id="EHS87050">
        <property type="protein sequence ID" value="EHS87050"/>
        <property type="gene ID" value="PS3_15781"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.658"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="8"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="281" mass="31833" checksum="F21A946965523131" modified="2012-04-18" version="1">MELNGAKFREIRRERGLSQIDLSEGICTQATISLIENSNRIPKLEILNQLVDRLDVPLSAILVVDRLDNGLLEQITNSMYQNKLGEVNHLLEEIDSNSLDGDDLTRYYYLMGFKELRQGNYDDAIYNLGLVLTQLSVNSSRQDYWPAVYYGFAAAYSRKGDLDKAQEMVDRSMKDLTHFMEGARHRNYGFWLAISVAQLQLELNQPDKSLQVCQAIIKVALNKRQLSPLGKAYHVMGQVNLSQGDSQLARENITIANSFAKVLKDQVELKETAALLDSIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ33</accession>
    <name>H4GJ33_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP synthase subunit delta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP synthase F(1) sector subunit delta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">F-type ATPase subunit delta</fullName>
            <shortName evidence="6">F-ATPase subunit delta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">atpH</name>
        <name evidence="7" type="ORF">PS3_10384</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="6">This protein is part of the stalk that links CF(0) to CF(1). It either transmits conformational changes from CF(0) to CF(1) or is implicated in proton conduction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ATPase delta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122066.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10384"/>
    <dbReference type="EnsemblBacteria" id="EHS87058">
        <property type="protein sequence ID" value="EHS87058"/>
        <property type="gene ID" value="PS3_10384"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.670"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01416">
        <property type="entry name" value="ATP_synth_delta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026015">
        <property type="entry name" value="ATP_synth_OSCP/delta_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020781">
        <property type="entry name" value="ATPase_OSCP/d_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000711">
        <property type="entry name" value="ATPase_OSCP/dsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11910">
        <property type="entry name" value="PTHR11910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00213">
        <property type="entry name" value="OSCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00125">
        <property type="entry name" value="ATPASEDELTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47928">
        <property type="entry name" value="SSF47928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01145">
        <property type="entry name" value="ATP_synt_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00389">
        <property type="entry name" value="ATPASE_DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0139">CF(1)</keyword>
    <keyword evidence="2 6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="3 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="180" mass="20381" checksum="79877E38A8407008" modified="2012-04-18" version="1">MSLNKMTVANRYARAMFELVDAQGQLDQTYEELTNLRQVFLDNPTLASLLSGVNLSLKEKESLLQVLKQDASQPVTNLIQMVFDYGRMDTLVAIINEFERRYDLKYKRVHADVVTAVQLDETRRNQLKANLAERLGANEVLLSEKVDPAIIGGVIVQTENQTMDGSIATQIEQVRRLLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ40</accession>
    <name>H4GJ40_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_15825</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122073.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15825"/>
    <dbReference type="EnsemblBacteria" id="EHS87065">
        <property type="protein sequence ID" value="EHS87065"/>
        <property type="gene ID" value="PS3_15825"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.679"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00036">
        <property type="entry name" value="HTHLACI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="2"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="314" mass="35334" checksum="C0B31B7A41D17069" modified="2012-04-18" version="1">MITIKDIAKCAGVSVSTASRALNHNSRISQKTIDRVEKIANELGYQPNQSAKTLSSGESTAVGVIFPVTEKDAPANPFHLDLIRGMNTELQTMGRVLSVAICQTSEALLTNVKQMVEQAKIHHFLVLYSYQDDPVTQYLRESGQNFVVVGTPDNPKDRFVDNNNLLAGKKATSFLLEQYQVHHPLFIRSTGHWNYEKNREEGYRSTLPETDQLVLQISEQTTNQQLRQFVEDHPEIDGIVAADDIGLLIFYHNLGFLPEIYQVPTVCFNSSRLIDMAGLKVDKVDMQPKLIGQQAVKLLFKPQQHQKLIGFEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ51</accession>
    <name>H4GJ51_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Multidrug ABC transporter ATP-binding and permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_6163</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122084.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6163"/>
    <dbReference type="EnsemblBacteria" id="EHS87076">
        <property type="protein sequence ID" value="EHS87076"/>
        <property type="gene ID" value="PS3_6163"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.691"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="16"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="333"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="367"/>
            <end position="374"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="581" mass="64684" checksum="9A0829E964E2727A" modified="2012-04-18" version="1">MIRIARKYFNWLAVTIAVVCLCIQVASDLYLPTVTSDLINKGVLQQDFNYIWHQGAIMLLVAFVGLAGSAVNVYFASTQSMKVGQKLRQDIFKHVLTFSSKEMSDFGDSSLITRSTNDVVQVQNVLVQMLRMMLMSPIMLVGAIIIAYNQEPRLTKIFLISLPILALAMIIIMVFAVPLFKSIQTKTDRINLVFREGLTGVRVIRAFNQDQREQDWFRQANADYTNTGIKAFTLVSFLMPIMTLILSMTNVGITWLGAHYIANMSMQVGNLVSFMTYSTQIMISFMMLSMIFVFVPRASASAARINQVLDTDSSVVDDPENEVALDVEQAADLKFDHVDFRYEGADELALTDLNFDVKAGQTLAIIGGTGSGKSTLVNLIPHLFNIERGRIEVNHQDITKLSQHDLHSLISITQQKAILFTGTIRSNLLFGNPNATEEDMWRVLEIAQARDFVEETGGLDAIVEENGDNFSGGQRQRLAIARTLIKPASIYIFDDSFSALDFKTDAKLRAALREDSAMQSAINVIVAQRVSTVVDADLILVLDDGKVVGQGTHAELKAHNQVYQEIIDSQIQEGDVERAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ73</accession>
    <name>H4GJ73_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Polyphosphate kinase</fullName>
            <ecNumber evidence="6 7">2.7.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP-polyphosphate phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Polyphosphoric acid kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ppk</name>
        <name evidence="13" type="ORF">PS3_4947</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>[phosphate](n) + ATP = [phosphate](n+1) + ADP</text>
            <dbReference type="Rhea" id="RHEA:19573"/>
            <dbReference type="Rhea" id="RHEA-COMP:9859"/>
            <dbReference type="Rhea" id="RHEA-COMP:14280"/>
            <dbReference type="ChEBI" id="CHEBI:16838"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="PTM">
        <text evidence="6 7">An intermediate of this reaction is the autophosphorylated ppk in which a phosphate is covalently linked to a histidine residue through a N-P bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the polyphosphate kinase 1 (PPK1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.1" evidence="6 7"/>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122106.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4947"/>
    <dbReference type="EnsemblBacteria" id="EHS87028">
        <property type="protein sequence ID" value="EHS87028"/>
        <property type="gene ID" value="PS3_4947"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.722"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009358">
        <property type="term" value="C:polyphosphate kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008976">
        <property type="term" value="F:polyphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00347">
        <property type="entry name" value="Polyphosphate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003414">
        <property type="entry name" value="PP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041108">
        <property type="entry name" value="PP_kinase_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024953">
        <property type="entry name" value="PP_kinase_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036830">
        <property type="entry name" value="PP_kinase_middle_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025200">
        <property type="entry name" value="PPK_C_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025198">
        <property type="entry name" value="PPK_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036832">
        <property type="entry name" value="PPK_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30218">
        <property type="entry name" value="PTHR30218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02503">
        <property type="entry name" value="PP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13090">
        <property type="entry name" value="PP_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17941">
        <property type="entry name" value="PP_kinase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13089">
        <property type="entry name" value="PP_kinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015589">
        <property type="entry name" value="PP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140356">
        <property type="entry name" value="SSF140356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03705">
        <property type="entry name" value="poly_P_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 6 7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PP_kinase_N" evidence="10">
        <location>
            <begin position="11"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="PP_kinase" evidence="9">
        <location>
            <begin position="126"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="PP_kinase_C_1" evidence="12">
        <location>
            <begin position="330"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="PP_kinase_C" evidence="11">
        <location>
            <begin position="505"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="686"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="697"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphohistidine intermediate" evidence="6">
        <location>
            <position position="434"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="563"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="591"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS87028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="714" mass="82287" checksum="0F38AE1C63EB8C88" modified="2012-04-18" version="1">MTKNFAKTEYFKNRELSWIDFNGRVLQEARDKMNPLLERVNFLGITQSNVDEFFMVRVASLTKMEAAKVESTDASGMTPQAQIIAINEAEHQAVRQRYSTYNKQLIPALADANIHLLSADQLTDGQAEFIERYFNDEIYPILTPMADDSSRPFPFISNNSLNLGVLLKDEAGNNDYATLRVPTGVVNRLVKLPEAENDFILLEEIIKPFLGRLFTGYTIKKVMAYRLIRDMDLDVAERDASDLLMAVQDQLKERERGHVVRLEIDGKMTDKLCRRLVKKIHVDERAIYEVNGPIDLTFLKKLPSRISEHDDLKYQPFQSYQEPALDKDHDIFAAMRQQDYLMQHPYDTFDSVVNFIHQAATDEQVLAIKMTLYRVSKQSPIIRDLGLAAQRGKQVTVLVEVKARFDEDNNVHWAQQLEKMGCHVIYGLVGLKTHSKIALVIRRDEDGIRRYLHLGTGNYNDSTARFYTDMGLLTCNRDMGMDATTLFNMLSGYSQHQYLHKIHQSPKLIRQFINQKIDEEIAAAQAGNKALIKMKMNSLSDKHIIEKLYEASAAGVKIHLLIRGICCLRTDLPGVSDNIKVHSIVGRFLEHSRIYYFYNNGNEDVYLSSADMMTRNLNRRVELLFPVLQTDLHDRVLRIFDILWRDNVKTRVLHDDQFERVNRRNQDPLDAQQYFVNEAEQKVQALKASEQDKSSSEFQPLTKHPDDLSLDSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ79</accession>
    <name>H4GJ79_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_10770</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10770"/>
    <dbReference type="EnsemblBacteria" id="EHS87034">
        <property type="protein sequence ID" value="EHS87034"/>
        <property type="gene ID" value="PS3_10770"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.734"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221">
        <property type="entry name" value="PTHR33221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02082">
        <property type="entry name" value="Rrf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51197">
        <property type="entry name" value="HTH_RRF2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="145" mass="16130" checksum="C267321FE820C4DB" modified="2012-04-18" version="1">MIMKFSYKFSDAIHLLAYLDIYQNGDLSSRRIADSIEANPSVVRNLMRDLKKAGLILTHQGSATPSLAKQPAKISLYDIYTAVDINHDLIHVDPNTNPACIVGGNIQTTLQDAYDRIQQAAFNEMKAISLQDIIDGILNNQQRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJA0</accession>
    <name>H4GJA0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Major tail 2 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000031">
        <property type="protein sequence ID" value="EHS87012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13785"/>
    <dbReference type="EnsemblBacteria" id="EHS87012">
        <property type="protein sequence ID" value="EHS87012"/>
        <property type="gene ID" value="PS3_13785"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.778"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022345">
        <property type="entry name" value="Phage_69_Orf23_MTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011855">
        <property type="entry name" value="Phgtail_TP901_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06199">
        <property type="entry name" value="Phage_tail_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01997">
        <property type="entry name" value="MTP2FAMILY"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02126">
        <property type="entry name" value="phgtail_TP901_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="210" mass="22692" checksum="7C765EBB2F677AD0" modified="2012-04-18" version="1">MSEIEALQGIDTLLFARKLSEASKTDGMLIPYQTSLKLSPKRKSDTTKTKSGTVAVSGGLETELEAEFIANWAKIADQLDDSIFNNEVMEFWIVFRKRVNADNKFYAYYMRGYVTENPLEGKADDAATRNPTITVIDTPQRGWTSLPNDIGAQIAYVFRGVGKIEGAAKNDGTDGNGKAWNKDTDPGTGSEPASAASSVTSPASSSPDAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJA7</accession>
    <name>H4GJA7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">L-lactate dehydrogenase</fullName>
            <shortName evidence="6">L-LDH</shortName>
            <ecNumber evidence="4 6">1.1.1.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ldh</name>
        <name evidence="12" type="ORF">PS3_1303</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of lactate to pyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>(S)-lactate + NAD(+) = H(+) + NADH + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:23444"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the LDH/MDH superfamily. LDH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.27" evidence="4 6"/>
    <dbReference type="EMBL" id="AICN01000032">
        <property type="protein sequence ID" value="EHS86997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122140.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000032.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_1303"/>
    <dbReference type="EnsemblBacteria" id="EHS86997">
        <property type="protein sequence ID" value="EHS86997"/>
        <property type="gene ID" value="PS3_1303"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.799"/>
    <dbReference type="UniPathway" id="UPA00554">
        <property type="reaction ID" value="UER00611"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:glycolytic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
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        <property type="entry name" value="L-lactate_DH"/>
    </dbReference>
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        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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        <property type="entry name" value="Ldh_1_C"/>
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    </dbReference>
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        <property type="entry name" value="Ldh_1_N"/>
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    </dbReference>
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        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="SSF56327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01771">
        <property type="entry name" value="L-LDH-NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00064">
        <property type="entry name" value="L_LDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ldh_1_N" evidence="10">
        <location>
            <begin position="7"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Ldh_1_C" evidence="11">
        <location>
            <begin position="147"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="122"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="150"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6 7">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="81"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 8">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="120"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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    <feature type="binding site" evidence="6">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
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        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>substrate</name>
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        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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    <feature type="modified residue" description="Phosphotyrosine" evidence="6">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004843"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
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            <dbReference type="HAMAP-Rule" id="MF_00488"/>
        </source>
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        </source>
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        </source>
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            <dbReference type="RuleBase" id="RU003369"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EHS86997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="319" mass="34505" checksum="9033492CADBE2220" modified="2012-04-18" version="1">MSISRQKVVLVGDGAVGSAYAFAMVQQGIAEEFAIINHTHEKAVGDVLDLEDATAYTHNKKIYAAGYDTCKDADIVVLTAGAAQKPGESRLELVSRNLKITKDIVDQVMASGFDGIFLVAANPVDILTYATQKFSGLPEKRVIGSGTSLDSARLQVALSKVFDVHPSNVIAYMLGEHGDTEFASFNNATIGGVPLLSLAEFNNVPMDQLKKIEDEVRHKAYEIINRKGATYYGVATSLARITRAILRDDDEILPVSAPMNGEYGLNDIYIGTTAVINRTGICEVFELPLFDEEAKSMAHSAAVLKEITDNGMKQLEAEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJC8</accession>
    <name>H4GJC8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Proteinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_3356</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000034">
        <property type="protein sequence ID" value="EHS86969.1"/>
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    </dbReference>
    <dbReference type="RefSeq" id="WP_007122161.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000034.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3356"/>
    <dbReference type="EnsemblBacteria" id="EHS86969">
        <property type="protein sequence ID" value="EHS86969"/>
        <property type="gene ID" value="PS3_3356"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.830"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M10" evidence="3">
        <location>
            <begin position="87"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="222" mass="25006" checksum="CAFA2CC07045BE48" modified="2012-04-18" version="1">MSPIKFKRFFTTIIYAGILIGVIWLYRYNQPFQRSIDNFTSAAQITINNWINPKTTQTDATNASETTSENGSESGQWTSTSATLYLDLNNQTLDQATREAIQAWNDTGAFTFKITTNRKKADVVITTMDNNSNGAAGLTKMNINSVTHYFVNGKMYLNQAYLLSPEYNYSYQQIVNTAEHELGHVIGLSHTDEISVMQPAGSLYSIQQLDIDNVQKFIINEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJF6</accession>
    <name>H4GJF6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prophage Lp2 protein 18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_18883</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000036">
        <property type="protein sequence ID" value="EHS86879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122189.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000036.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS86879">
        <property type="protein sequence ID" value="EHS86879"/>
        <property type="gene ID" value="PS3_18883"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.902"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007731">
        <property type="entry name" value="DUF669"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05037">
        <property type="entry name" value="DUF669"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="248" mass="27208" checksum="CCBAAB0342DF46DE" modified="2012-04-18" version="1">MLFTTNSNDVFSGTYVEEAGEYNVKVLPTTTLTQTKTNGNPMITLNYEVIDGKYQGGQIRYDNQTWVDYDQEAHAQSIKRFNTLLKATGIKDGIKIENMQQLVNLLIGSTMSIKVDWEQGNNGYYNLRVKQHNPVLPSGSKPNGVKAPDSGNSNSSNNGFNTTFNSTYNTPQGNQNSQWGNNQSQSQNGLGGQNGANNDLFNDLFAGQPQSLKDQALANQDQPVDTANLPFDNPQGQSAGDQIQEVPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJH8</accession>
    <name>H4GJH8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable transcriptional regulatory protein PS3_6964</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_6964</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TACO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000040">
        <property type="protein sequence ID" value="EHS86607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122211.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000040.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6964"/>
    <dbReference type="EnsemblBacteria" id="EHS86607">
        <property type="protein sequence ID" value="EHS86607"/>
        <property type="gene ID" value="PS3_6964"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.933"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.980">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00693">
        <property type="entry name" value="Transcrip_reg_TACO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017856">
        <property type="entry name" value="Integrase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002876">
        <property type="entry name" value="Transcrip_reg_TACO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026564">
        <property type="entry name" value="Transcrip_reg_TACO1-like_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029072">
        <property type="entry name" value="YebC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12532">
        <property type="entry name" value="PTHR12532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01709">
        <property type="entry name" value="Transcrip_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75625">
        <property type="entry name" value="SSF75625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01033">
        <property type="entry name" value="TIGR01033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS86607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="243" mass="26668" checksum="C5E4DC5259FAE544" modified="2012-04-18" version="1">MSGHSKWHNIQGRKNAQDAKRGKIFQKISRDLYQAARAGDPDPANNPQLRLVMDKARAANMPKQNIQRAIDKATGAGGANFEEITYEGYGPGGVAVMVSCLTDNKNRTASAIRSAFTHSGGSLGANGSVSYMFNRQGLIEILREDLDKDEDEMLMDALDAGADDMQADEEMFHIYTDPSSMTAVRDALQADGYELETAEVTMIPENKTEVPSDKVSQYQHLIDELEENDDVADVYEAGFIPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJI3</accession>
    <name>H4GJI3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FeS assembly protein SufD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_8991</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0051 (ycf24) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000041">
        <property type="protein sequence ID" value="EHS86573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122216.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000041.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8991"/>
    <dbReference type="EnsemblBacteria" id="EHS86573">
        <property type="protein sequence ID" value="EHS86573"/>
        <property type="gene ID" value="PS3_8991"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.942"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011542">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43575">
        <property type="entry name" value="PTHR43575"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="SSF101960"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01981">
        <property type="entry name" value="sufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SufBD_N" evidence="2">
        <location>
            <begin position="95"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="424" mass="46638" checksum="A1F149FA6820D690" modified="2012-04-18" version="1">MTFDFEKMSQVVAANSRKRQEPSWLSQKRQAGLQALTTAPAPKMQKFDFRDWDLMPLPDLSATSDLDEATELDKAHLVQVGTTTPYVNLPDDVRQQGVILTDLATAAEQYADLVQPALMTVVSADENQLTAYHQAMMNNGIFVYVPQNVEVDIPLETMIYQDATNPNPLVHHVLIVTEANAKLQFVEHLATLGEEKTMASLMVEIIAKENSQVSFASLDELGINTTEYFKRRAQIGRNAKVEWSIGLMSDGNVVGDVDSELVGTGGMADSKTIAVSTRQQEIGINNRVTHHGQNTEGLINQRGVLLEQSKLVFNGIGEIIHGAHGAEAEQQNRVLMMSPDAHGDANPILLIDENDVIAGHAASVGPVDPEQMYYLVSRGIPQSQAERLVIRGYLSPVITAIPSKLLRQKMIDILERKLAHGQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJI4</accession>
    <name>H4GJI4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Cysteine desulfurase</fullName>
            <ecNumber evidence="4 6">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_15792</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L-selenocystine to produce L-alanine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="4 6"/>
    <dbReference type="EMBL" id="AICN01000041">
        <property type="protein sequence ID" value="EHS86574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122217.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000041.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15792"/>
    <dbReference type="EnsemblBacteria" id="EHS86574">
        <property type="protein sequence ID" value="EHS86574"/>
        <property type="gene ID" value="PS3_15792"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.943"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006534">
        <property type="term" value="P:cysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06453">
        <property type="entry name" value="SufS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010970">
        <property type="entry name" value="Cys_dSase_SufS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01979">
        <property type="entry name" value="sufS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_5" evidence="7">
        <location>
            <begin position="26"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="411" mass="45181" checksum="BA3EC4ED4A4EB908" modified="2012-04-18" version="1">MDKTSEEIRHDFPTLDQMVNQAPLAYLDNAATSQRPAPVLKAVEDFYFHDNANVHRGVHTLAQRATDRYEQARETVRQFLGAESPTEIIFTKGCTEALNLVASSYGMSQIQAGDEIVISIQEHHSNLIPWQQLAIQKGAILKYIDLTDEGEIDLTDAQEKITDQTKLVAINHASNVMGTITPLKTLVELAHQKGAVVVADGAQAVLHESVNVRDLDVDFYAFSGHKMLAPMGIGVLYGKASLLEKMPPYQYGGEMISRVQRQESSWAQAPYKFEAGTQNVAGAIGLAAAIDYLNNLGWEWIQQQESELIQYATQELTKVAGLKLYGPKEAQRRSGVFSFNLGQIHPHDVATALDMAGVAVRAGHHCAQPLMEYLGVPATVRASFAFYNTKAEVDQLVAGLQATKEFFDHGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJK1</accession>
    <name>H4GJK1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription antiterminator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_18962</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122234.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18962"/>
    <dbReference type="EnsemblBacteria" id="EHS86511">
        <property type="protein sequence ID" value="EHS86511"/>
        <property type="gene ID" value="PS3_18962"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.973"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.24.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004341">
        <property type="entry name" value="CAT_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036650">
        <property type="entry name" value="CAT_RNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011608">
        <property type="entry name" value="PRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036634">
        <property type="entry name" value="PRD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03123">
        <property type="entry name" value="CAT_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00874">
        <property type="entry name" value="PRD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01061">
        <property type="entry name" value="CAT_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50151">
        <property type="entry name" value="SSF50151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63520">
        <property type="entry name" value="SSF63520"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51372">
        <property type="entry name" value="PRD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRD" evidence="1">
        <location>
            <begin position="63"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="PRD" evidence="1">
        <location>
            <begin position="170"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="282" mass="32299" checksum="5604D787774FF953" modified="2012-04-18" version="1">MKFVKNFNNNAALVEDDQQTEWIVIGKGVGFGKHRGDEIDLAKIERRFKAADQEETVLNTATQLSPEIVEVVNRVEKLVKDQLSRSFTDYQYLSLADHIDFAIKRTRDGINLDSATVSWETNRLFPKEYAVAKEALMLINGMTGAGLDEGEAVMLTQHFVNLESNGTTIQDTMKISRLIKGIIDIVQYQYQIQLDPDSFNFTRFIGHLRAFMIRQLTNSKVNGQALDQSLMMVIQEKYPDAVACVDRIAVFLKNQMGWDLSPDERVFLILHVWRVTNYQEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJK4</accession>
    <name>H4GJK4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_5341</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122237.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5341"/>
    <dbReference type="EnsemblBacteria" id="EHS86514">
        <property type="protein sequence ID" value="EHS86514"/>
        <property type="gene ID" value="PS3_5341"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.978"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027785">
        <property type="entry name" value="UvrD-like_helicase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13538">
        <property type="entry name" value="UvrD_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="7">
        <location>
            <begin position="207"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="228"/>
            <end position="235"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="763" mass="87807" checksum="31510BD9F4602186" modified="2012-04-18" version="1">MTDPKRYEQEHLDHVIDEIRRAKVATEEKIGHTKTDIGQIKEGFDDIRMNTTTYSGMMDTAMSVRAQQQMLDERENTWQHAADLLETLERLESRPYFARIDFKETDDAEAETIYIGLASFTDQPDHFLVYDWRAPISSIYYEGELGKVSYQAPDGVQTVDVQLKRQFQIEDGVIVTLYDTEEAVTDQMLLSALDNHSSTKMKSIVTTIQRTQNQIIRNTDARLLFVQGAAGSGKTAAVLQRVAWLLYRYRGNLTASQVIMFSPNQLFNDYIDQVLPELGEHNMIQMTYYQYVGRRVPRLQVATIQERFEADQEVVHQKATKLLTSLKYFNAVTRYAKHLGLDHHLRFKNIMFRGRVFFSKEKIAEIYYSFNRNYHLGNRLDATKESLIKILNSRIGNEMRSKWVEEAIQTMSKEDLDVALHEHPGEFESEEAERKFLARRIVMDALRPVKKAIDHNRWMNINAQYLHLLRVTPKLVNIADFDISPAEWQQYLDQQTMAFKEKRISTSGVSIYLYLYDLLTGKRGQREIRFVFVDEVQDYDAFQLAYLQFNFPKAKFTLLGDLNQAIFTHEQSHTLLQELGTMFGEDNTKVIQLTKSYRSTQQLTDFTKGLLVNGERIEAFERQGELPRVLELADESTAVATVVNTLDRYQAAHDTTAIIGKTLADSERIAELLKAAGEQVTLIRTENQRLVPGTIVVPSYLAKGLEFDAVIVWNANAKQYHGDAERQLIYTICSRAMHALTVTSIGPVSPLIDEVDASLYQRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJK9</accession>
    <name>H4GJK9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_6497</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122242.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6497"/>
    <dbReference type="EnsemblBacteria" id="EHS86519">
        <property type="protein sequence ID" value="EHS86519"/>
        <property type="gene ID" value="PS3_6497"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.986"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="280" mass="31947" checksum="7C6F6E074AD80639" modified="2012-04-18" version="1">MFTFLDMINSSLGYFNMSVKVKNRIYTIVALLGDGYLIYVTFMLLKNQSWLRGFLYCLAVIVITYYLYLNIVYYYLGRTSRWDFLSPKLTQLTGFSPETPEEKDRKRLARREAMLAMNQSNGLYANEDVIQTEVKISQQEQQNLRDVVDDLVARKLIAVDYNGLDAEGITEHFRSTGEGVPAVNKGQQPPFFELEHNSQTGQYEVYIGINQMERRPVGHITKVGLTEINTAAKAYELYLANVYITGGPQKMPGRSGSPILTDADFGLIVQVAYRNRQAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJM1</accession>
    <name>H4GJM1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Asparagine--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Asparaginyl-tRNA synthetase</fullName>
            <shortName evidence="7">AsnRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">asnS</name>
        <name evidence="9" type="ORF">PS3_5725</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + H(+) + L-asparaginyl-tRNA(Asn)</text>
            <dbReference type="Rhea" id="RHEA:11180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9659"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.22"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000043">
        <property type="protein sequence ID" value="EHS86436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122254.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000043.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5725"/>
    <dbReference type="EnsemblBacteria" id="EHS86436">
        <property type="protein sequence ID" value="EHS86436"/>
        <property type="gene ID" value="PS3_5725"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1000"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00534">
        <property type="entry name" value="Asn_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="8">
        <location>
            <begin position="132"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS86436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="432" mass="50087" checksum="C708844EDFE033BE" modified="2012-04-18" version="1">METINIIDAPKHVNERVRIGVWLTDKRSSGKIAFLQLRDGTAFFQGILLKNKVSEEIFEMARHDLRQESSFWVTGEIHEDARSKFGYEIQIDDIELIGDSEDYPIGNKEHGVDFLLDHRHLWLRSSKPQAMMKVRDRVIRATYEFFGQQGFTKIDPPILTGNAPEGTTDLFHTEYFDQDAYLSQSGQLYEEAGAMALNKVYSFGPVFRAEKSKTRRHLIEFWMIEPEMAFMHQEESLEVQEQYVAYLVSAVLKDCRHELEVLGRDPESLVPFTKLPYPRISYDEAVEMLKKANFEIEWGVDFGSPEETYLADQFDQPVFILNYPKSIKPFYMKGHPTRDDVYVCADLLAPEGYGEIIGGSERETDYEVLRQAILDAGLDESAYEWYLDLRKYGTVPHSGFGLGLERFLTWITKTDHVRETIPFPRLLNRLYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJM2</accession>
    <name>H4GJM2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptidase propeptide and ypeb domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000043">
        <property type="protein sequence ID" value="EHS86437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13430"/>
    <dbReference type="EnsemblBacteria" id="EHS86437">
        <property type="protein sequence ID" value="EHS86437"/>
        <property type="gene ID" value="PS3_13430"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1001"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041401">
        <property type="entry name" value="DUF5590"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17881">
        <property type="entry name" value="DUF5590"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="SSF54403"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5590" evidence="1">
        <location>
            <begin position="28"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="146" mass="16295" checksum="6D4272ED83580822" modified="2012-04-18" version="1">MVVLVACGWGFYASANHDKTVAQNRVTALAKSKANLTTVSNFFIYNQERTYYSVAGVNKKNQQLLVIVPAGSSKVTVLKQSSGITGREAIQVVQKKHQIRRVLHTALGIYRKSPVWEVAYYNTKGKLNYALVNFKTGKIVEEMNNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJR5</accession>
    <name>H4GJR5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_13422</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000047">
        <property type="protein sequence ID" value="EHS86215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122298.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000047.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS86215">
        <property type="protein sequence ID" value="EHS86215"/>
        <property type="gene ID" value="PS3_13422"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1071"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="371"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="443"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS86215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="501" mass="55122" checksum="9E3F3A196CFCC02A" modified="2012-04-18" version="1">MEEKQQSSVVTKISLGGLILMIFSSIFGWNNSLTAFYQMGYASILWYLVAAILFFVPSSLMFAEYGAAFKAARGGIFSWLRGSIGEKPAFIGTFLWLAAWVVWLVSSSQYFLVAVSTMIFGKDVTTSWHLFGLSSDLTLGILEVLFLVIVTFIATHGIDKIVKVTSFAGIFTMAMPIAFLVISAIILIMHHGTLAEPITTTNLIHSPNQNFVSPIAVVSFLVYAIFAFGGMETMAGVIDNVDQPEKTFPKGVLIGCGMMACLYFLVIFLCGISTNWNAILGKSNVNLANVEYVIINNLGLEMGKGFGMSHANSLLLGQIFSHIAAFADVFGGIGAAFVMTYSPIKSFVQGCDQRLLPKRFTTMNQHGMPAFAMWCQVAVVSCIIMFIACGGAAASTFYTILTDMMNVGSSTPYLFLIGAFPFFKMKEGLDRPFVFYRRMSFTWVVTIIVWLVIAIGIIFTIIQPIIEGEYMTSFWTAFGPVFFGVVAWIFYTRAEKRGTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJS6</accession>
    <name>H4GJS6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cystathionine gamma-lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_17329</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122309.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17329"/>
    <dbReference type="EnsemblBacteria" id="EHS86113">
        <property type="protein sequence ID" value="EHS86113"/>
        <property type="gene ID" value="PS3_17329"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1095"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="PTHR11808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="376" mass="41914" checksum="565840CEE7D192A0" modified="2012-04-18" version="1">MCSQGKKSVETIAAQAGNHRDANGSVSAPLYFSSNYRHPSLEQAINFDEQKEYTYARLATPTRRVLEETLAELESGQRAFALSSGMAAVQLALSILKTGDELISLDDLYGGDFRYFRYLESHMGVKFKQWDGEKLADLVDLLSPATRVVWLETPSNPTMKKLDLKALSAAVHEYNSSILVMVDNTFYTPIYQQPLTLGADVVVHSATKYLSGHNDLLGGVVVVNDEQLDNEYYEYYITTGDTMATFDAWLLLRSLKTLPIRMERHTKSATKIKEFLESRPEVTKVLYPGKAGMISFYLATPELTRQVLDNVKIISFAESLGGAESLITIPLYQTHADVEEAQREKLGITPTLLRLSVGLENPDDLIADLAQLLDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJU4</accession>
    <name>H4GJU4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dak phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_15383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122327.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15383"/>
    <dbReference type="EnsemblBacteria" id="EHS86131">
        <property type="protein sequence ID" value="EHS86131"/>
        <property type="gene ID" value="PS3_15383"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1122"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004371">
        <property type="term" value="F:glycerone kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019986">
        <property type="entry name" value="DAK2_dom-cont_prot_YloV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004007">
        <property type="entry name" value="DhaL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036117">
        <property type="entry name" value="DhaL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033470">
        <property type="entry name" value="UPF_DhaK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13684">
        <property type="entry name" value="Dak1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02734">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01121">
        <property type="entry name" value="Dak1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01120">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101473">
        <property type="entry name" value="SSF101473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03599">
        <property type="entry name" value="YloV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51480">
        <property type="entry name" value="DHAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DhaL" evidence="1">
        <location>
            <begin position="9"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="562" mass="60228" checksum="74447EAC1F9ED4CE" modified="2012-04-18" version="1">MSVTEITNLEFGQMVQAAAAKLNNQAEFINSLNVFPVPDGDTGTNMSMSMASGAKYERESTSDQVGDLATALAKGLLMGARGNSGVILSQIFRGFSKGCEGKATLSPTDFVNAYANGAQTAYKAVMKPTEGTILTVVRESAQAGLDAIKAGDADLAIIMKKIVDAADAALAKTPDLLPVLKEVGVVDSGGQGLDFVLHAFYEALTGEVIDEIKPDNAQIEEMINVQHHQSAQGKLNPEDIEFGYCTQMMVRIGHGNSVTKQFDYEEFYNYLAGMGDSLLVINDDEVVKVHVHTEHPDQVLGWGLQFGDLQTIKIDNMRWQQEEIMQNDNQVATTMSDKPAEESVADTAVIAVSSGDGISKLFQSLGVTKIIAGGQTMNPSIQDLTDAINQSGAKQAIVLPNNGNIFMAAEQAAEVADIPTVVVHAKTIAQGMSALLEYNPDADLDTNHDAMEGNLSLVKSGEVTNAVRDTTIDGQTIKKDDYMGIVDGKIVVTKPTVAEAAIAMVKEMLDDESEIVTILYGQDGSSEVAEQIEAAIEEIDDEVEVQIYEGNQPVYPYLISVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJW0</accession>
    <name>H4GJW0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Asparagine synthase (Glutamine-hydrolyzing)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_14293</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asparagine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000050">
        <property type="protein sequence ID" value="EHS86034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122343.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000050.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14293"/>
    <dbReference type="EnsemblBacteria" id="EHS86034">
        <property type="protein sequence ID" value="EHS86034"/>
        <property type="gene ID" value="PS3_14293"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1143"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_Synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="8">
        <location>
            <begin position="2"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="5">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="7">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS86034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="530" mass="60547" checksum="35C869FC24C8ABEA" modified="2012-04-18" version="1">MCGFLTVSAEKYDMTKFKQALACDERRGPDMTWVEDQGLVKFGFNRLAIMDLSADGMQPFQYDGVTLVCNGEIYNYHELEDNLKDDYQFKSTSDCEVLIPLYKKYGMDVMCKMLDAEFALVLYDQTTQQVLAARDPIGIRPMFYGYLTETDQIAFASTAKSLMEICDRIYPFPPGHYYDGEKFVCYNDPAFSPKIRTASVAEATEGIRLRLVDAVQKRIHADAPLGYLLSGGLDSSLVCAIAKKLSPDVPMRTFAIGMDRNPIDLKYAREVADYLGTDHTEFIMTREDVLGALRDVIYALETWDITTIRASLGMYLLCKKIHENTDLKVLMTGECSDEMFGYKYTDYAPSPAEFQKESQKRIRELYMYDVLRADRCISSNSLEGRVPFADRDFVRYVMSLDPAMKMNVYGKGKYLLRQAFAGQDYLPDDILLREKAAFSDAVGHSLVDDLKEYAESQYTDEDVAKAGEKYAYCTPFTKESLLYRDIFEEFFPGKAEWIKDFWMPNKEWDALKDVDDPSARVLKNYGDSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJX7</accession>
    <name>H4GJX7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polyprenyl synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_6153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS85994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122360.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6153"/>
    <dbReference type="EnsemblBacteria" id="EHS85994">
        <property type="protein sequence ID" value="EHS85994"/>
        <property type="gene ID" value="PS3_6153"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1170"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="325" mass="35770" checksum="B52DE5EBECF2113C" modified="2012-04-18" version="1">MSRSFIPQYPELNTNLQAVADLITNRIQTADHQLETALKTMANNGGKYIRPGFVCLFSMIKGPNDQEQLLKAAASIEILHMATLIHDDIIDDAPQRRGAISIQAAFGKDIAVYSGDYLFTVFFQLITEAFANTPLLKVNATVMSKILNGELGQMAHRFDLNQSVTTYLDNIKGKTAALFSLAAREGAFLGNLSEQEVQIAQVIGEKVGMAFQILDDILDYSDGHDMNKPVLEDLTTGVYSLPLLIALANDPSHAIRDLLAKQSQMTMDDLLQIRQLVIANDGVKIAHQTAQQYSNDALALLADLPDSKATRNLKKLINKLLNRTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJY6</accession>
    <name>H4GJY6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphoserine aminotransferase</fullName>
            <ecNumber evidence="6">2.6.1.52</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Phosphohydroxythreonine aminotransferase</fullName>
            <shortName evidence="6">PSAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">serC</name>
        <name evidence="9" type="ORF">PS3_12415</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-oxoglutarate + 4-(phosphooxy)-L-threonine = (R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16573"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58452"/>
            <dbReference type="ChEBI" id="CHEBI:58538"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-oxoglutarate + O-phospho-L-serine = 3-phosphooxypyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:14329"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="6">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 6 7">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 6">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.52" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS86003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122369.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12415"/>
    <dbReference type="EnsemblBacteria" id="EHS86003">
        <property type="protein sequence ID" value="EHS86003"/>
        <property type="gene ID" value="PS3_12415"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1190"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00197"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004648">
        <property type="term" value="F:O-phospho-L-serine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00160">
        <property type="entry name" value="SerC_aminotrans_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022278">
        <property type="entry name" value="Pser_aminoTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247">
        <property type="entry name" value="PTHR43247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000525">
        <property type="entry name" value="SerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01364">
        <property type="entry name" value="serC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0718">Serine biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_5" evidence="8">
        <location>
            <begin position="4"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="6">
        <location>
            <begin position="76"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="6">
        <location>
            <begin position="236"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS86003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="361" mass="39868" checksum="34BE03816250DA00" modified="2012-04-18" version="1">MDKVYNFSAGPATLPHEVVLKAQAELLSFKDSRMSVMEISHRSDLYTTMASEAEQDLRDLMQIPDNYEVLFLQGGATQLFYTLPLNFANQVHRVGYINTGHWSERAEEEARYVSGLAVEEVGSSADQHFTELVHHVTLNGDYDFIHLTTNNTIEGTMYRDLPGLNGKTLVADLSSNFLGQEYNVNDFGIIYAGAQKNSGIAGLTVAIVRKDLLEQVPDLPAMLSFSKQAAKHSALNTPPVFAIYMAGLVFKWIKEQGGVAAMQKRNEAKAQLLYEFLDSSNLFRNPVVSADRSTMNIPFVTGDTDLDALCVQEATKQGLMSLKGHKSVGGLRASLYNAMTIDGVQALVKFLEEFEKEHGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJY7</accession>
    <name>H4GJY7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Hydroxymethylglutaryl-CoA synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_8057</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. HMG-CoA synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS86004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122370.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8057"/>
    <dbReference type="EnsemblBacteria" id="EHS86004">
        <property type="protein sequence ID" value="EHS86004"/>
        <property type="gene ID" value="PS3_8057"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1192"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013746">
        <property type="entry name" value="HMG_CoA_synt_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011554">
        <property type="entry name" value="HMG_CoA_synthase_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08540">
        <property type="entry name" value="HMG_CoA_synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01835">
        <property type="entry name" value="HMG-CoA-S_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG_CoA_synt_N" evidence="4">
        <location>
            <begin position="3"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="HMG_CoA_synt_C" evidence="5">
        <location>
            <begin position="180"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="HMG_CoA_synt_C" evidence="5">
        <location>
            <begin position="257"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="2">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR611554-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR611554-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS86004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="386" mass="42488" checksum="EC894C467EF88342" modified="2012-04-18" version="1">MQIGIDKMAFATTPYYLDLVDLAQARGEDHNKYLIGIGQNKQAVVPPTQDIVTLAAEAASRLLDDHDREDISTVIVATESGIDNSKAAAIYVKQLLDLDDFVRTIEVKEACYSGTAAIQFARGLVALDPQQKVLVIAADIARYGLHTGGEVTQGAGAVAMLISANPQMLAIESGSVALSQDVMDFWRPIYATEAMVDGKYSTNVYIDFFQRTFNRYCQRQNRQLSDFAALAFHLPFTKMGKKALEAELGDQQDATSQRLRANLVAEQAIPREVGNLYTGSLYLGLMSLLVNGDVQPGERIGLFSYGSGAEGEFYSGILQPSYQDGLKAASLALDHRQRVTMSEYEALFNQQLGIKAEDVDFDLSQDPSRFVLTGQRDHQRQYLSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK10</accession>
    <name>H4GK10_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0473 protein PS3_13774</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_13774</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0473 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000054">
        <property type="protein sequence ID" value="EHS85862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122393.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000054.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13774"/>
    <dbReference type="EnsemblBacteria" id="EHS85862">
        <property type="protein sequence ID" value="EHS85862"/>
        <property type="gene ID" value="PS3_13774"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1236"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01448">
        <property type="entry name" value="UPF0473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009711">
        <property type="entry name" value="UPF0473"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40066">
        <property type="entry name" value="PTHR40066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06949">
        <property type="entry name" value="DUF1292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="100" mass="11779" checksum="CD6C3C8F59F4C7C1" modified="2012-04-18" version="1">MTEPRDEQEVFTLVDENGNEELYMELMRFQSDETGKWYICLYPAEEENADEVSIQAFAFEQTGDEEDDEIELQPIENDVEWEMVQEVLNTFIDDEGNVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK11</accession>
    <name>H4GK11_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Ribonuclease HIII</fullName>
            <shortName evidence="12">RNase HIII</shortName>
            <ecNumber evidence="5 12">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">rnhC</name>
        <name evidence="15" type="ORF">PS3_7778</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 12">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the RNase HII family. RnhC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="5 12"/>
    <dbReference type="EMBL" id="AICN01000054">
        <property type="protein sequence ID" value="EHS85863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122394.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000054.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7778"/>
    <dbReference type="EnsemblBacteria" id="EHS85863">
        <property type="protein sequence ID" value="EHS85863"/>
        <property type="gene ID" value="PS3_7778"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1237"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14796">
        <property type="entry name" value="RNAse_HIII_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00053">
        <property type="entry name" value="RNase_HIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004641">
        <property type="entry name" value="RNase_HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024568">
        <property type="entry name" value="RNase_HIII_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="PTHR10954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11858">
        <property type="entry name" value="DUF3378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037748">
        <property type="entry name" value="RnhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00716">
        <property type="entry name" value="rnhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3378" evidence="14">
        <location>
            <begin position="2"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="RNase_HII" evidence="13">
        <location>
            <begin position="90"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF11858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EHS85863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="301" mass="33109" checksum="61BEB581DFE670E2" modified="2012-04-18" version="1">MNVVIQVDPATFQKMQATYPSQGNLPTGASFQAKLKGVTVTGYTKSRKVMFQGQLAQEEAQRWQANPVQKKANLTKDPSLPADFSHWSVLGSDEVGAGSYFGPLTTAAVYVAANQVDQLTQLGIQDSKKLTDPQIIKLAEQIIALCPYTYLNLMPEAYNQRMQKYNQAQLKALCHNIVLAKTLEKIAPARPQAILIDQFVQAKTYYHYLTGQPQIVKDKVYFRTKGEQVHVAVAAASIVARYYSLQAMDALSNDAGMTIPIGANHAVDEVAAKLIRQGQQLGHFAKLHFGNTQKAQAIANQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK36</accession>
    <name>H4GK36_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">D-alanyl-D-alanine</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PS3_18571</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase S11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000055">
        <property type="protein sequence ID" value="EHS85852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18571"/>
    <dbReference type="EnsemblBacteria" id="EHS85852">
        <property type="protein sequence ID" value="EHS85852"/>
        <property type="gene ID" value="PS3_18571"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1270"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015956">
        <property type="entry name" value="Peniciliin-bd_prot_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018044">
        <property type="entry name" value="Peptidase_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037167">
        <property type="entry name" value="Peptidase_S11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001967">
        <property type="entry name" value="Peptidase_S11_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00768">
        <property type="entry name" value="Peptidase_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00725">
        <property type="entry name" value="DADACBPTASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69189">
        <property type="entry name" value="SSF69189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="6" id="KW-0133">Cell shape</keyword>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase_S11" evidence="12">
        <location>
            <begin position="4"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="9">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR618044-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS85852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="388" mass="41930" checksum="09DE24A513EAE447" modified="2012-04-18" version="1">MGATTAKAAAIDANAALAVDASTGQVLYQQNASQSLPIASMSKLLTMIVIEQEIKAGKLSWNSQLKITQAEAELSENTEYSNITLEAGKSYTVKELVTAALLKSADAATITLSRATGDNTAEFAQKMTKAARKIGVKDAKIYNAVGLTNEQMGTFKTKGVAADAENQMSAKDVALIAQYVIKKYPDLLKITQQSSATVAGQTVENTNSLLTGFTYHNQQVKIDGLKTGTSDQAGNCFVSTGKYRGHRLITVIMHAGGSDSSNRFTQTQKLYETVLGSYSPHWLTAKYSVKVAHAKSKKVKLVYQSASAIWVKKGTKLKQPKIQLLKVYQTKSHKLKAPVKTSDQVANAVYQQAQGLNGGKLKVALYPEKSLPKLNAFMDFFRTITGQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK37</accession>
    <name>H4GK37_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_12391</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000055">
        <property type="protein sequence ID" value="EHS85853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122420.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000055.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12391"/>
    <dbReference type="EnsemblBacteria" id="EHS85853">
        <property type="protein sequence ID" value="EHS85853"/>
        <property type="gene ID" value="PS3_12391"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1271"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="157"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="381" mass="43026" checksum="3E5EFE008EBF4DB6" modified="2012-04-18" version="1">MLKLTRREKSSLILEGILTIILLGLLNLAVLTILQILMVNQPGVNLGFFVMQQSVAVGPIRMRIVGAQQILVILMLVADFVILWWRMVRRYHQYQMDHIISELHYIAQGHLEHRIPFSVKGAQQHVITSVNALVDSTARSIKEEREIEKSKDELITNVSHDLRTPLTSIIGYLGLIENHQYRSEEEIVQFANIAYNKAQQMKSMVDDLFEYTKVQFNAEKPKFVEVDLTQLLEQVSASFELEAEKHGIQMQTDFVQSPLMVEVVPDKIGRVFSNLVSNALKYGDGSSYIRIATQVKDDQVDITVANDGVPIPEESVQHLFERFYRVESSRSKQTGGTGLGLAIVQSIIETHHGHVGVKSDESATTFTVTIPLQQPKDQKGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK44</accession>
    <name>H4GK44_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_16436</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000056">
        <property type="protein sequence ID" value="EHS85811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122427.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000056.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16436"/>
    <dbReference type="EnsemblBacteria" id="EHS85811">
        <property type="protein sequence ID" value="EHS85811"/>
        <property type="gene ID" value="PS3_16436"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1280"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="3"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="5">
        <location>
            <begin position="132"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="200" mass="22601" checksum="FD1690B138947EE5" modified="2012-04-18" version="1">MVKIALIDDHEMMLNSLAKAMQEQANLEVVGTFTDVASFQLFLKTTEVDLVIMDMMLKDETAFQVLENLQKSLSDMPKVILISGFYDELLHQRALKLGVQAFLRKESSYDELMRAIQEVMAGNVLIPDKVFNQPDDEKLTEVELAVLQAMADEKTNPQIAEELFMSQRTVESHVTNICRKLGVSGRIGAVREALKRKLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK56</accession>
    <name>H4GK56_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">23S rRNA (Uracil-5-)-methyltransferase RumA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_3779</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000059">
        <property type="protein sequence ID" value="EHS85690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122439.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000059.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3779"/>
    <dbReference type="EnsemblBacteria" id="EHS85690">
        <property type="protein sequence ID" value="EHS85690"/>
        <property type="gene ID" value="PS3_3779"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1301"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034470">
        <property type="term" value="P:ncRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030390">
        <property type="entry name" value="MeTrfase_TrmA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030391">
        <property type="entry name" value="MeTrfase_TrmA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061">
        <property type="entry name" value="PTHR11061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01230">
        <property type="entry name" value="TRMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01231">
        <property type="entry name" value="TRMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRAM" evidence="6">
        <location>
            <begin position="6"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="411"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="411"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="455" mass="51595" checksum="EF29D276064BDAA0" modified="2012-04-18" version="1">MKVKIPVRKGEELTGQIEDLTYQGNGVMKVDHYPIFIPEALPEEKVTVKVTKVTTNFAWGQVLNWQSKSPDRVADPDQRYLQTGIAPLGHLKYPAQLRFKQKLIQDLLQKAHLAVDVAPTMGMDKPFQYRNKAQVPVRFIRGQIETGFYKRGSHELVPIENFYIQDPVIDETIKIVRDLIRQYHIRAYDEVKQTGDLKTIMVRRGYYSHQVMVVLVTRTTKLPHQAELVQGIVDQVPGITSIIQNINSKPTNVVLGRENHTLWGAETIHDQLLGIDFTISPLSFYQVNPQQTERLYQTAIDYAELDGNQTVIDAYCGIGTISLAVAKHAKKVYGVEIVEAAIQDAKLNAQHNQIENAEFVVGKAEEQMAKWQAQGLKPDVLIVDPPRKGLDERVIEAAGQMTPQKIVYISCNPATLVRDLKRFMDQGYRVTKPIQPVDQFPQTTHVESITLLERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK66</accession>
    <name>H4GK66_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LacI family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_8349</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000061">
        <property type="protein sequence ID" value="EHS85627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122449.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000061.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8349"/>
    <dbReference type="EnsemblBacteria" id="EHS85627">
        <property type="protein sequence ID" value="EHS85627"/>
        <property type="gene ID" value="PS3_8349"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1312"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00036">
        <property type="entry name" value="HTHLACI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="5"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="327" mass="36855" checksum="5C26113EB2D654A9" modified="2012-04-18" version="1">MNQRVKLDDVAEKAGVSKTTVSRVLNHRGYLSDQTIKKVHQAMEELHYRPNVVARHLYHQKTNQVGLVFPTVNDPFFAHLESRLAYELYERGYATLMGNSQNNPDKERRFLHYLLDGQIDGLIVGAHNQGLSEYQETNLPIVSIERWVAPKIPIVASDNYAGGLMATQRLLDDGCQNIIHTNYPRLESSTNQRRQQAYEDLMIQHHRQPITYEVNFDSPVEDKVAIFKKIFAEHPEVDGIVADNDTNASLIIHLAQELGYQVPDQLKVVGFDGADTTQILMPELTTIKQPINDLASTAVDLLIKRINGEENVASVILPVTLHEGTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK70</accession>
    <name>H4GK70_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycine/betaine ABC transporter permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_7729</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000061">
        <property type="protein sequence ID" value="EHS85631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122453.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000061.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7729"/>
    <dbReference type="EnsemblBacteria" id="EHS85631">
        <property type="protein sequence ID" value="EHS85631"/>
        <property type="gene ID" value="PS3_7729"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1319"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="16"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="208" mass="22096" checksum="6C1D18E7A79E9318" modified="2012-04-18" version="1">MISYWQANSSMILTDTWQHLQLVGGALIIALIIATELIVLCLGHDRWLTVLNHVFSLAYSIPSFALFALLIPITGLGRLTAVIVLVVYCEYVLLRSFVSGIESVDPILIEVAKGMGMTDTQVLIKVQLPLALPSIFGGLQIALASTMGIATIAATINAGGLGELLFAGLQSQAMAPILWGIIATVLLTLIGAGILKMIERVLVSDKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK83</accession>
    <name>H4GK83_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Abhydrolase_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_15819</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122466.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15819"/>
    <dbReference type="EnsemblBacteria" id="EHS85535">
        <property type="protein sequence ID" value="EHS85535"/>
        <property type="gene ID" value="PS3_15819"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1342"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Abhydrolase_3" evidence="2">
        <location>
            <begin position="90"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="309" mass="35159" checksum="55D3D295514FC941" modified="2012-04-18" version="1">MKLKKQVLTKPRHRSPKANMVELGIQLSGIKRQFEDPSKFAVYLNQQATKVKKPFAFPKGTHFHVNYEIKAFEGLSYIVLNQQLRPAKRLVYFCGGEYAERPTKDHWNFLDQLAQVTKAQIYAPIVPTIAQGGAPVAYPWVRKFYEHLLDLDRHVPTMMLGDSAGGGLAAGFVSTLIDQNLLLPDQLVLISPWLDLSLANSAISDYEKSDVYLSVAGLQRIGQRWQGEWEQNDPRLSPVEADWTPEVPVQIFVGNKEIMYPDCLRFAEKLRQQHVSVDLQIGQQMFHDYPIYPTPEGQHVIKEIADFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKA9</accession>
    <name>H4GKA9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="8">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gltX</name>
        <name evidence="12" type="ORF">PS3_8637</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122492.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8637"/>
    <dbReference type="EnsemblBacteria" id="EHS85561">
        <property type="protein sequence ID" value="EHS85561"/>
        <property type="gene ID" value="PS3_8637"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1377"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033910">
        <property type="entry name" value="GluRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="SSF48163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1c" evidence="10">
        <location>
            <begin position="5"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_2" evidence="11">
        <location>
            <begin position="361"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="259"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS85561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="499" mass="57694" checksum="9B65F37247665450" modified="2012-04-18" version="1">MAKNKVRVRYAPSPTGHLHIGNARTALFNYLFARHYKGQFIIRIEDTDTKRNIEDGERSQLDNLKWMGLDWDEGPDKGGDFGPYRQSERRDLYDQYIQQLLDQDLAYKSYMTEDELEAVRERQKANHEMPHYEYEYAGMSADEIASAQAAAEAKGLKPVIRFRVPNDAVYEWDDLVKGHLSFESSSVGGDFVIQKRDGMPTYNFAVVVDDHLMQITHVFRGDDHVSNTPKQMMIYEALGWDVPKFGHMSLIINTETGKKLSKRDESILQFIEQYRDLGYLPEALVNFIVLLGWSPEGEDEIFSLKEFVKMYDEKRLSKSPAAFDRKKLRWINNQYMKLSSADEVFNAAMPQLIEAGLMEKHADPYQMEWMRRLVELFKREISYAHEIIDYVKPFVHGPADIDDDAKEEMKAETAVVVLKAFRDKIAAMDFMDATSVLAAIKAVQKETKIKGRQLWMPIRIAVTHESHGPELPESIELFGQKTTLEHLDTMIDRIENNQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKB0</accession>
    <name>H4GKB0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">DNA repair protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">radA</name>
        <name evidence="8" type="ORF">PS3_10558</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent ATPase involved in processing of recombination intermediates, plays a role in repairing DNA breaks. Stimulates the branch migration of RecA-mediated strand transfer reactions, allowing the 3' invading strand to extend heteroduplex DNA faster. Binds ssDNA in the presence of ADP but not other nucleotides, has ATPase activity that is stimulated by ssDNA and various branched DNA structures, but inhibited by SSB. Does not have RecA's homology-searching function.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Plays a role in repairing double-strand DNA breaks, probably involving stabilizing or processing branched DNA or blocked replication forks.</text>
    </comment>
    <comment type="domain">
        <text evidence="5">The middle region has homology to RecA with ATPase motifs including the RadA KNRFG motif, while the C-terminus is homologous to Lon protease.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the RecA family. RadA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122493.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10558"/>
    <dbReference type="EnsemblBacteria" id="EHS85562">
        <property type="protein sequence ID" value="EHS85562"/>
        <property type="gene ID" value="PS3_10558"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1378"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01121">
        <property type="entry name" value="Sms"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01498">
        <property type="entry name" value="RadA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004504">
        <property type="entry name" value="DNA_repair_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041166">
        <property type="entry name" value="Rubredoxin_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18073">
        <property type="entry name" value="Rubredoxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00416">
        <property type="entry name" value="sms"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5 6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0346">Stress response</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RECA_2" evidence="7">
        <location>
            <begin position="69"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Lon-protease-like" evidence="5">
        <location>
            <begin position="354"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RadA KNRFG motif" evidence="5">
        <location>
            <begin position="255"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="98"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="457" mass="49540" checksum="2AD4570B41ACC97D" modified="2012-04-18" version="1">MAKAKVHYVCQNCGYNSPRFLGRCPNCGEWNTLVEEVEQTSVAAATKATTTLTGIVARPQKINEIDTKKTPRVKTKLRELNRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLSLENHPVLYISGEESANQIKMRAQRIAVSGDNFYIYPETNMDSIRATIDNLKPEYVVIDSVQTMQASGVESAIGSVAQIRAVTAQLMQIAKGENITVFVVGHVTKGGTIAGPKILEHMVDTVLYFEGDSHHTYRILRSVKNRFGSTNELGIFEMHDTGLTEVANPSEMFLEERLKDATGSAVVVSLEGTRPILVEVQALITPTVFGNAQRTASGLDRNRVSLIMAVLEKRANLLLQNQDAYLQAAGGVKLNEPAIDLALAISIASSYRDKGTRPTDAFIGELGLTGEVRRVSRIEQRVAEAAKLGFKRVFIPKNNLQHWTPPTNIEVVGVATINEALKLALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKB3</accession>
    <name>H4GKB3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Multidrug ABC transporter ATP-binding and permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_15067</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122496.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15067"/>
    <dbReference type="EnsemblBacteria" id="EHS85565">
        <property type="protein sequence ID" value="EHS85565"/>
        <property type="gene ID" value="PS3_15067"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1382"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="28"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="348"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="381"/>
            <end position="388"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="586" mass="65075" checksum="CDFADEA7682228E7" modified="2012-04-18" version="1">MSVREKSTEKFSLAGIARYFKPYTVMLIVAALLSIVGSILNLIGPRLISQITNYISAGLHGTIKLSTIGHLGMILAIMYALGFVFNYVQGYMMATIAQRVSQDMRIDISRKINRLPLRYFDQNSTGDILSRVTNDVDTVGQSMNQSLSTVISSLTMFFGSAIMMFATNWLMAIAGIVTALLGFTIMMFVIARSQKYFKQQQDLLGEVNDQVDETFSGHLVVKAYNGEQYEKGRFANKNQRLYQAAWKSQFLSGMMMPMMIFVGNLAYVVVCIVGAVLAVQGSISFGTIVAFMLYIRLFTQPLQNLSQAVSSVQSMAAASQRVLEFMQQPELADESGKVASIEPVEGQVDFDHVSFGYDPDQVIIKDFNEAVQPGQKVAIVGPTGAGKTTIVNLLMRFYETNQGQIRIDGHNTADLKRALVHDQFAMVLQDTWLFEGTLRDNLVYNREHISDQQLDTVMEAVGLTDLVEQLPQGYDTVLLDDEMLSAGQKQLITIARAMLKDAPLLILDEATSSVDTRTELKVQKAMDKLMTDKTSFVIAHRLSTIRDADLILVMNHGNVIESGTHEELLAQEGFYADLYNSQFEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKB4</accession>
    <name>H4GKB4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Multidrug resistance ABC superfamily ATP binding cassette transporter, ABC/membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_12407</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12407"/>
    <dbReference type="EnsemblBacteria" id="EHS85566">
        <property type="protein sequence ID" value="EHS85566"/>
        <property type="gene ID" value="PS3_12407"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1383"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="277"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="11"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="330"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="364"/>
            <end position="371"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="577" mass="63167" checksum="8EB91C05473AF20E" modified="2012-04-18" version="1">MSKREWGYVGISIVFIVLQVWLDLKLPSYMSAITTLVETSGSKMSDILLQGGYMLLCALGSMAAATITGYFVAKVAAGLSRTLRSLTYNQILDFSNAEIGHFSTASLINRSTNDIVQIQNLVAMGLQAIVKAPIMAIWALTLIIDKSWQWTASTGIAVLVLVVMLALVLLVAVPRYRRVQSLTDNLNRVTREQLTGIRVVRAYNAERYEEEKFDQANDELTNNNLVANRVMALMSPTMTLINSGLTLAIYWIGAYLIEAASLSSKLPLFSDMVVFSNYAMQVIMAFMLLNMIFILLPRAQVSAKRIIQVLTTPISIQDGQGGTPKRQGQVEFKHVSFTYPGAQESSLSDVSFSVKPGSTLAIIGATGSGKTTLVNLINRLYDVTDGQVLVDGQDVRDYQLAELRQRVSLAPQKATLFKGTIASNVSYGDRGTGEISSTQIDMSLAAAQATEFVQQKAEGINEPISQGGTNVSGGQRQRLSIARSLAGNAEIMIFDDTFSALDYRTDAQLRHDLNQEFTQETKIIVAQRIGTIRDADQILVLDHGQIVGQGTHEELLANSPEYLEIAESQLSPDELKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKG6</accession>
    <name>H4GKG6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Exodeoxyribonuclease III</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_13786</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000067">
        <property type="protein sequence ID" value="EHS85380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122548.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000067.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13786"/>
    <dbReference type="EnsemblBacteria" id="EHS85380">
        <property type="protein sequence ID" value="EHS85380"/>
        <property type="gene ID" value="PS3_13786"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1491"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008853">
        <property type="term" value="F:exodeoxyribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020848">
        <property type="entry name" value="AP_endonuclease_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="PTHR22748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00727">
        <property type="entry name" value="AP_NUCLEASE_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00728">
        <property type="entry name" value="AP_NUCLEASE_F1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endo/exonuclease/phosphatase" evidence="3">
        <location>
            <begin position="5"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="278" mass="31465" checksum="BF31FBA90D6CFA40" modified="2012-04-18" version="1">MKLISWNIDSLNAALTGTSDRSEQTRAVLKTIAAQDPDTIAIQETKLGASGLTKKHQAGLAEFFPGYQYVWRSSEEPARKGYAGTLYLYKDEYTPTVTYPVIDAPSPMDQEGRIITLEFPDFFLTEVYTPNAGNGLKRLAEREVWDDRFREYLQSLDQEKPVLVSGDLNVAHEEIDLAHPGSNHHSAGFTDEERSHFTKLLAAGFTDTFRQLNPDAVGKYTWWAQRSLTSKVNNSGWRIDYWLVSNRLADRVTNSTMLDSGERRDHTPIMLEIDLQAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKJ9</accession>
    <name>H4GKJ9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_15364</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122581.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15364"/>
    <dbReference type="EnsemblBacteria" id="EHS85279">
        <property type="protein sequence ID" value="EHS85279"/>
        <property type="gene ID" value="PS3_15364"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1544"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032254">
        <property type="entry name" value="DUF4828"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16110">
        <property type="entry name" value="DUF4828"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS85279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="120" mass="13454" checksum="D15EEA9B6553B065" modified="2012-04-18" version="1">MRKNSLLVSSIGLVAGISSSLGFTQLKRKIKPLTSLPVLYAGNWKYVDGTRNRTHTISISPKLNLTIDDQAIPANVEHINSQELTFVDKFGYRITIQTNQERPVKLIDEADDQAYNIEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKK7</accession>
    <name>H4GKK7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_16862</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122589.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16862"/>
    <dbReference type="EnsemblBacteria" id="EHS85176">
        <property type="protein sequence ID" value="EHS85176"/>
        <property type="gene ID" value="PS3_16862"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1561"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="412"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="26"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS85176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="441" mass="47922" checksum="C5FDFD011145C15E" modified="2012-04-18" version="1">MENNLSQSLDFSLPRKTLKKDSVKTVILASMVGTMIEFYDFYAYGTASAAYFPRVFFPEVTPTIALLLSLLTFGVAFIARPLGSFVFGHYGDKMGRKKTLVISLLLMGCSTVLIGLLPGYNTLGLTAVVLLCLCRFVQGIGLGGEWSGAALVATENAPANKRALYGAFPELGAPLGFFLSNGLFVVLEFTLSPSQMESFGWRIPFLASAVLVVVGFWVREKMQETPLFRMAKQRSATTKSPLVEVFKSNWLEIVQGTFIVAVTYTLFYTLATWSLSYATTTLGFSKQAYLMMLMGTVVIFALLILFASRISDAFGRKRVLLFSAVALVIFGLVFPYLLTGHHNVWGALTFLGVGFALMGIAFGPLGAVLPELFPTKVRYSGSGIAYNLAAIVGAAFAPTIAAWLAANWGIHSVGLYLSLMAVLCVVSLLTMHETKEVDFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKL2</accession>
    <name>H4GKL2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Glutamine--fructose-6-phosphate aminotransferase [isomerizing]</fullName>
            <ecNumber evidence="2 8">2.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">GFAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Glucosamine-6-phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Hexosephosphate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">L-glutamine--D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">glmS</name>
        <name evidence="11" type="ORF">PS3_12665</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="2 8"/>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122594.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12665"/>
    <dbReference type="EnsemblBacteria" id="EHS85181">
        <property type="protein sequence ID" value="EHS85181"/>
        <property type="gene ID" value="PS3_12665"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1569"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901137">
        <property type="term" value="P:carbohydrate derivative biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00164">
        <property type="entry name" value="GlmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GlmS_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="PTHR10937:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="initiator methionine" description="Removed" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="9">
        <location>
            <begin position="2"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="287"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="458"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for GATase activity" evidence="8">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="active site" description="For Fru-6P isomerization activity" evidence="8">
        <location>
            <position position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS85181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="606" mass="67114" checksum="CD119CC6958AB541" modified="2012-04-18" version="1">MCGIVGVTGNDRSVEILINGLKTLEYRGYDSAGIYVNDQNGHDYLVKRQGRIKNLESALTDDVHGKAGIGHTRWATHGEPSEKNAHPHFSNDKRFYLVHNGVIENYEQLKREYLGEVELNSQTDTEVIVQLVAHFVHESHMTTKSAFLKVLRLISNESSYAFVLMDREEPDTLFVAKNKSPLLVGLADGYNLVGSDAISMLKETNRFMEIEDHELVIVKPDRIQIEDFEGQERSRDSFVVDMDADAADKGAYPYYMLKEIDEQPAVMRRIVGQYFDQAGNPLVDVAILDALAKADHLYIIGAGTSYHAGLVGARMIEKYAHIPTTVHISSEFAYDHPMLSEHPFFLFLSQSGETADSRQVLVEVNENHWPSLTMTNVDKSTLSREATYTMLLHAGPEIAVASTKAYTAQIAVEAVLAKALGQFLKHPEADEFNLRSQLGMVAYGMQAITDSKDDIEEIASRFLSKTRNAFYIGRAIDWSVSIEAALKLKEISYVQAEGFASGELKHGTIALIENGTPVIGIITQDKTAGLTRSNLEETVARGANAITIASRHLAQETDTIIIPDVDDNLTPLLSVVPAQLLAYYTSLGKGYDVDKPRNLAKSVTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKM3</accession>
    <name>H4GKM3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SsrA-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Small protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">smpB</name>
        <name evidence="4" type="ORF">PS3_10188</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="3">The tmRNA-SmpB complex associates with stalled 70S ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SmpB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122605.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10188"/>
    <dbReference type="EnsemblBacteria" id="EHS85192">
        <property type="protein sequence ID" value="EHS85192"/>
        <property type="gene ID" value="PS3_10188"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1580"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070929">
        <property type="term" value="P:trans-translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09294">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00023">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023620">
        <property type="entry name" value="SmpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000037">
        <property type="entry name" value="SsrA-bd_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020081">
        <property type="entry name" value="SsrA-bd_prot_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30308">
        <property type="entry name" value="PTHR30308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01668">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74982">
        <property type="entry name" value="SSF74982"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00086">
        <property type="entry name" value="smpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01317">
        <property type="entry name" value="SSRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="156" mass="18302" checksum="7D331F6F37791840" modified="2012-04-18" version="1">MAKQHQSNHENLVAQNKKARHDYAVTDTYEAGLVLTGTEIKSIRDRRVNLKDGYAQFHHDELWLMNVHISEYAGGNIFNHDPLRNRKLLLHKKELKKIRGEISVKGITLVPLKMYLKHGYAKVLLGVAKGKHEYDKRNDIKKREQDRQIERVLKHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKN7</accession>
    <name>H4GKN7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chaperone protein DnaJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dnaJ</name>
        <name evidence="10" type="ORF">PS3_10149</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 2 Zn(2+) ions per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DnaJ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122619.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10149"/>
    <dbReference type="EnsemblBacteria" id="EHS84957">
        <property type="protein sequence ID" value="EHS84957"/>
        <property type="gene ID" value="PS3_10149"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1597"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:protein folding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
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        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DnaJ"/>
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        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="SSF49493"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="SSF57938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02349">
        <property type="entry name" value="DnaJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <keyword evidence="5 6 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="5"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="9">
        <location>
            <begin position="137"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="6">
        <location>
            <begin position="150"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="6">
        <location>
            <begin position="167"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="6">
        <location>
            <begin position="193"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="CXXCXGXG motif" evidence="6">
        <location>
            <begin position="209"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="7">
        <location>
            <begin position="137"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS84957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="381" mass="41568" checksum="F3F8307DF4C64CE7" modified="2012-04-18" version="1">MAEKDYYETLGVDKDASEQDIKRAFRKLAAKYHPDVNKEPGAEEKFKEINEAYETLSDPQKRSQYDQFGSAGPQGFGGGQGGFGGFGGGAQGGFSDFSDIFGDLFGGGRTRRDPTAPKPGRDLQYTMTLDFMDAVFGKESQIKYNRETNCDVCHGSGAKPGKSAHTCSQCHGTGYIVTQRQTMMGVMQSQQVCPTCGGTGQEIDPADRCDKCHGVGHIQERHEVKVTIPAGVDDGQQLRLQGQGEVGKNGGPYGDLYIVFRVTPSRDFKRDGTTIYVEEDITIPQATLGDKIQVKTVHGNVELSIPAGTQSETDFRLRGKGVPILHGNGNGDEYVKVHVKTPKKLNKRQREAMMAFAEASGEEVEGVKGGFWEKLKDAFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKR2</accession>
    <name>H4GKR2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Phosphatidate cytidylyltransferase</fullName>
            <ecNumber evidence="13">2.7.7.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PS3_13730</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H(+) = a CDP-1,2-diacyl-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.7.7.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the CDS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="13"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122644.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13730"/>
    <dbReference type="EnsemblBacteria" id="EHS84982">
        <property type="protein sequence ID" value="EHS84982"/>
        <property type="gene ID" value="PS3_13730"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1635"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000374">
        <property type="entry name" value="PC_trans"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01315">
        <property type="entry name" value="CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="6"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EHS84982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="262" mass="29008" checksum="356D32648ADE8B1E" modified="2012-04-18" version="1">MKTRVITAVIALIVFIPFILYGGLPLTIFAMALSLIAMSELLVMKKMFLTSFAAILSFLGVLVVAMPSSWVAWMPDQLGRMSLLYIIVILLLAHTVFHRQRFTFEDTGVLVLGMIYIGFGFNAFVIARSVNFQTLLYGMLIVWLTDSFAYICGRKIGKHKLAPKVSPNKTWEGSIAGTVIATVVLAIYLLIFPVGYHNVFMMILMTLVLSIIGQIGDLIESALKRYYGVKDSGKILPGHGGILDRFDSMLVVLPAMYILGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKS2</accession>
    <name>H4GKS2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UPF0154 protein PS3_17328</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_17328</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the UPF0154 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17328"/>
    <dbReference type="EnsemblBacteria" id="EHS84992">
        <property type="protein sequence ID" value="EHS84992"/>
        <property type="gene ID" value="PS3_17328"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1648"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00363">
        <property type="entry name" value="UPF0154"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005359">
        <property type="entry name" value="UPF0154"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03672">
        <property type="entry name" value="UPF0154"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS84992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="81" mass="9221" checksum="D3C1D5167D3149BA" modified="2012-04-18" version="1">MRREEITVGITITVAIIALLVGAIAGFYGARKYMENYLRNNPPISEDMLRTMMMQMGQKPSDRKLHQMMQTMKAQAKKSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKS3</accession>
    <name>H4GKS3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0291 protein PS3_16193</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_16193</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0291 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122655.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16193"/>
    <dbReference type="EnsemblBacteria" id="EHS84993">
        <property type="protein sequence ID" value="EHS84993"/>
        <property type="gene ID" value="PS3_16193"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1649"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01103">
        <property type="entry name" value="UPF0291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009242">
        <property type="entry name" value="DUF896"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37300">
        <property type="entry name" value="PTHR37300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05979">
        <property type="entry name" value="DUF896"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="83" mass="9899" checksum="BA0AB72F820B3F04" modified="2012-04-18" version="1">MPESKEQTELLKRINELAHKNKAEGLTPAEKAERKKLRDQYLKNFREAFRSNIELMQIYDDNGKEVTPEKIKEIQRKRGLRDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKU8</accession>
    <name>H4GKU8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Homoserine kinase</fullName>
            <shortName evidence="11">HK</shortName>
            <shortName evidence="11">HSK</shortName>
            <ecNumber evidence="4 11">2.7.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">thrB</name>
        <name evidence="14" type="ORF">PS3_4587</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + L-homoserine = ADP + H(+) + O-phospho-L-homoserine</text>
            <dbReference type="Rhea" id="RHEA:13985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.39"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the GHMP kinase family. Homoserine kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.39" evidence="4 11"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122680.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4587"/>
    <dbReference type="EnsemblBacteria" id="EHS85018">
        <property type="protein sequence ID" value="EHS85018"/>
        <property type="gene ID" value="PS3_4587"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1677"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00064"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004413">
        <property type="term" value="F:homoserine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00384">
        <property type="entry name" value="Homoser_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000870">
        <property type="entry name" value="Homoserine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000676">
        <property type="entry name" value="Homoser_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00191">
        <property type="entry name" value="thrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0791">Threonine biosynthesis</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="12">
        <location>
            <begin position="70"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP_kinases_C" evidence="13">
        <location>
            <begin position="192"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS85018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="288" mass="30767" checksum="A705A48AC35E1A5E" modified="2012-04-18" version="1">MRIKVPASSANLGPGFDSMGMALGLYLTIDVLEPTDHWVVDHDLEQIPHDERNMVVRAALKIKSDLAPHHIVVNSPIPLAHGLGSSSSCNVGGIELANQIAKLHLSAEEKVEIASQIEGHPDNVAPTILGGTVIGTSLHDHFIAVKAPAMPYQFVAYVPAYNLKTSDSRNALPNMIGFHEATQASAIANTLVAALFAQDYQAVGELMEADMFHEPYRSKLVPELAQVRAIAHQHQALATYLSGAGPTVMTLLPKDASVDEFVQALKANGLDDPVMVLPIAEAGVEVDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKV6</accession>
    <name>H4GKV6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">ATP phosphoribosyltransferase</fullName>
            <shortName evidence="14">ATP-PRT</shortName>
            <shortName evidence="14">ATP-PRTase</shortName>
            <ecNumber evidence="5 14">2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">hisG</name>
        <name evidence="16" type="ORF">PS3_2948</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 14">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Heteromultimer composed of HisG and HisZ subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">Lacks the C-terminal regulatory region which is replaced by HisZ.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the ATP phosphoribosyltransferase family. Short subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17" evidence="5 14"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122688.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2948"/>
    <dbReference type="EnsemblBacteria" id="EHS85026">
        <property type="protein sequence ID" value="EHS85026"/>
        <property type="gene ID" value="PS3_2948"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1688"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13595">
        <property type="entry name" value="PBP2_HisGs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01018">
        <property type="entry name" value="HisG_Short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024893">
        <property type="entry name" value="ATP_PRibTrfase_HisG_short"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="PTHR21403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 16" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 14" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="10 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HisG" evidence="15">
        <location>
            <begin position="52"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EHS85026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="207" mass="23258" checksum="04363576E896CA14" modified="2012-04-18" version="1">MILKIALTKGRVEEQFMELLDKLQIDITPFRNKQRKLIIDLPGYQFILAKGPDVMTFVNRGVVDLGIVGSDLLMEQGNERNELMDLKIGQCQFILASVAGFDVNAPGRKTIGTRYPRVAARYFDRLGQDVEIIKIEGSVELAPLIQLTDAIIDITETGTTLRENNLVVYDRLTKVSTRLVANTMALRHKKTAILNFMHELSSVIPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKY2</accession>
    <name>H4GKY2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glutamine amidotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_6146</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000076">
        <property type="protein sequence ID" value="EHS84834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122714.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000076.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6146"/>
    <dbReference type="EnsemblBacteria" id="EHS84834">
        <property type="protein sequence ID" value="EHS84834"/>
        <property type="gene ID" value="PS3_6146"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1724"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011697">
        <property type="entry name" value="Peptidase_C26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044668">
        <property type="entry name" value="PuuD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43235">
        <property type="entry name" value="PTHR43235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07722">
        <property type="entry name" value="Peptidase_C26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS84834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="242" mass="26446" checksum="F5807C933391DDE7" modified="2012-04-18" version="1">MRKPIIAMPADTFTKATNIINLRNAAFAPDPAIQAITKSGGTPLILPTVAAESAPDYVHLFDGLLLLGGFDVDPTFFGEEPHYELGETFRKRDLFEIALLKATVEAGKPVMGICRGLQLINAGLGGTLYQDLSEDPSAKLKHHQLAPGNLPTHHVNTEPGSKLNELLGDRVYVNSRHHQAVKRIAPGLRITATADDGVAEAIESENSDQVVAVQWHPENMFKHNEEAQMLFKDLITRSQKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKZ7</accession>
    <name>H4GKZ7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bifunctional protein PyrR</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="5">Pyrimidine operon regulatory protein</fullName>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="5">Uracil phosphoribosyltransferase</fullName>
                <shortName evidence="5">UPRTase</shortName>
                <ecNumber evidence="5">2.4.2.9</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="5" type="primary">pyrR</name>
        <name evidence="7" type="ORF">PS3_9718</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Also displays a weak uracil phosphoribosyltransferase activity which is not physiologically significant.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Regulates transcriptional attenuation of the pyrimidine nucleotide (pyr) operon by binding in a uridine-dependent manner to specific sites on pyr mRNA. This disrupts an antiterminator hairpin in the RNA and favors formation of a downstream transcription terminator, leading to a reduced expression of downstream genes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>diphosphate + UMP = 5-phospho-alpha-D-ribose 1-diphosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:13017"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer and homohexamer; in equilibrium.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrR subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122729.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9718"/>
    <dbReference type="EnsemblBacteria" id="EHS84763">
        <property type="protein sequence ID" value="EHS84763"/>
        <property type="gene ID" value="PS3_9718"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1740"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01219">
        <property type="entry name" value="PyrR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023050">
        <property type="entry name" value="PyrR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2 5" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="6">
        <location>
            <begin position="4"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="PRPP-binding" evidence="5">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS84763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="176" mass="19689" checksum="9DF01EA055276877" modified="2012-04-18" version="1">MAREIVDGSSMQRALTRITYEIIEQNKGVDNLVFVGIKTRGVYLAQRLAKRMEQLEGVKVPVGSLDITLYRDDRHQADHTVEPTVTSADIDFDINDKHVILVDDVLYTGRTVRAALDALMDLGRPKRISLAVLVDRGHRELPIRPDFVGKNIPTAVNETVHVAVEEYDGNEGITLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL18</accession>
    <name>H4GL18_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase superfamily I metal-dependent hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_2741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122750.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2741"/>
    <dbReference type="EnsemblBacteria" id="EHS84784">
        <property type="protein sequence ID" value="EHS84784"/>
        <property type="gene ID" value="PS3_2741"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1768"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="17"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="268" mass="29710" checksum="D62AE9FF878FE794" modified="2012-04-18" version="1">MSLEKELKYSILASGSTGNSTYIETGQHRILIDAGLSGKKIEERLQAIGRSMTDIEAIFVTHEHSDHIQGVGVLARRYGVNVYANQGTWDAMSNKIGKVKSEQCFEVQPGQVIDFGDLQVESFAVSHDAAQPQFYQIHHDDKSLCLLTDTGYVSERMVGIIQNADAYLMECNHDTEMLRNGPYTWSLKQRILGDEGHLSNEEGAAALISVIGDRTKEVILGHRSQHNNLRSLAHLTVAEELETHDLGVDHDFKLIDAEPDQALGLTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL25</accession>
    <name>H4GL25_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic-di-AMP phosphodiesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_13413</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Has phosphodiesterase (PDE) activity against cyclic-di-AMP (c-di-AMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3',3'-c-di-AMP + H2O = 5'-O-phosphonoadenylyl-(3'-&gt;5')-adenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54420"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:71500"/>
            <dbReference type="ChEBI" id="CHEBI:138171"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="2">For phosphodiesterase activity, probably binds 2 Mn(2+) per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GdpP/PdeA phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13413"/>
    <dbReference type="EnsemblBacteria" id="EHS84791">
        <property type="protein sequence ID" value="EHS84791"/>
        <property type="gene ID" value="PS3_13413"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1776"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106409">
        <property type="term" value="F:cyclic-di-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014528">
        <property type="entry name" value="GdpP/PdeA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026583">
        <property type="entry name" value="YybT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0464">Manganese</keyword>
    <keyword evidence="1 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="4">
        <location>
            <begin position="161"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="359"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="431"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="456"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="512"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR026583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR026583-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS84791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="673" mass="76079" checksum="27AE6808CE448A75" modified="2012-04-18" version="1">MINRQNLDQFLRRTTVLWILVFAFVIAIIGIIIGLMYDWRIGASLAVIVLIGMIYSFARLQRINNDTEAYLNQLAVQVNQSQEEALLEMPIGMILLTDENIIKWINPYMAKYFDPQEMIVGRKLAMVDDDLAKLVAKHRHDDQTSVVEWQDYKFELQVQDQFNTVYLTDITDYEEISDRYQREQLFLGNIFLDNYDEITKGMSDSMVSNLSNYVTSQLSQWAKDHDLLLKVIDDDNYLLIGHNYSLKTLENENFDVLDTIRQNTSQQNSPVTISMGIAYGQSNLIRLANMAQKNLDLALGRGGDQVVVRMESGQARFFGGKTNPMEKRTRVRARMISQALRDLMNQADNIFVIGHTRPDLDSIGACFGIRRLAEMNDHESWIVLDDDETNHSDIERLMGEVDNYQSIKEHIINPEEALKKATDASLLIMVDHSKPSLSAFPKLTERLANRLVIIDHHRRGEEFPENPLLVYIEPYASSTCELITEMFEYQPRESKGLNRLEATAMLAGIRIDTKSFSQNAGTRTYDAASYLRSAGADGKMIQYLTKETPANFIQRNHLIDTAEFIDDAMAICAGADDQTYDSVIAAQAADMLLQVSGIQASFVITRQSPDIVGISARSIGDFNVQVIMERLGGGGHLTNAATQIKDTTVEAVKAQLITELTSDQEAESETEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL44</accession>
    <name>H4GL44_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="2 6">6.3.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_21018</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 6">
            <text>ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-cysteine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glutamate--cysteine ligase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="2 6"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122776.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21018"/>
    <dbReference type="EnsemblBacteria" id="EHS84674">
        <property type="protein sequence ID" value="EHS84674"/>
        <property type="gene ID" value="PS3_21018"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1799"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007370">
        <property type="entry name" value="Glu_cys_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006334">
        <property type="entry name" value="Glut_cys_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38761">
        <property type="entry name" value="PTHR38761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04262">
        <property type="entry name" value="Glu_cys_ligase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glu_cys_ligase" evidence="7">
        <location>
            <begin position="14"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Glu_cys_ligase" evidence="7">
        <location>
            <begin position="255"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="456" mass="50917" checksum="394FDAB1D1ABD247" modified="2012-04-18" version="1">MFSQIGETIFEHEVVAKTSDFNMGLEVEMHRADSQGRISSDPTPAGISLNSPWVTKDFTETMTEVVTPAASNSLDAIHYLYSIQNALRAALAPGEVLWPLSMPPAFADANKIELAQTTPEKHAYLERVLENMGRVNKGLPCGAHISLSIDDHVIDIIWEQNSGRFASKAALKNHLYLLLAQGFVRYRWVLTYLFGASPVAEAGFFDPEDDQPTGPIRCLRQSHYGFDMRFKGDYSTIQNYVERIEAGVANGEIIDEAQFHGAIRLKTSGDLTDMEKNGVSYIELRMLDLDPTSTTGVKLDTLRLLRIMASYFIMTPGMQTKDVSVTIQRANEINEEVATENPTTHSQYEIAGKNFLNRLFQFANQIQLGPEYLETLETMMDRFDNPANTISGQLVAHIENGSLLPYMTKRAAHYQDGALMAMRTFNGFADMETPLSAEDLKNNLFRGSYEPSGLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL48</accession>
    <name>H4GL48_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Competence protein EC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_9200</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9200"/>
    <dbReference type="EnsemblBacteria" id="EHS84678">
        <property type="protein sequence ID" value="EHS84678"/>
        <property type="gene ID" value="PS3_9200"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1803"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030420">
        <property type="term" value="P:establishment of competence for transformation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07731">
        <property type="entry name" value="ComA-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035681">
        <property type="entry name" value="ComA-like_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004477">
        <property type="entry name" value="ComEC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004797">
        <property type="entry name" value="Competence_ComEC/Rec2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03772">
        <property type="entry name" value="Competence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00360">
        <property type="entry name" value="ComEC_N-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00361">
        <property type="entry name" value="ComEC_Rec2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="422"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Lactamase_B" evidence="7">
        <location>
            <begin position="453"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="713" mass="81713" checksum="FA850624B0A08175" modified="2012-04-18" version="1">MIWRLMILVESPWRWLVILISIMFGVRAWIWQQEQIQLTHPPQAQVSQMVVVQPDQWKVNGDFVQAIGQGKRQNYLIQAYLKEPSQQKYLERLHNPVQVIIKGDATPIAPATNYNEFDRRFWYRTQGVQMVIKGQLINVQPVKNYSLVDRLHQVRHQLATYFHHLPAPLSGYALRLLIGLQDPATDEQMTTVKQLGLIHLFCLSGLHVASVCSLVQGGGRILGYDRHFLNWILIGTLPLYWVIGGGSLSLTRAILMIECQLWSQQLGRNQPDAWTISLLIQEFIQPTVLMSLGGQLSYLLSFALCWFDWQKVKWQSFKLGALGLPPLLHFVFEFHLLSLVINYLMIPFFSWIIFPGTIISALLYPFLPGISGSFNRLLVIFDQSLVWLAEFPGMIHFGKIGVLLTLIPLIITWWTIEHATGRSWIILLLCYGGIYLGIHRPLSGEVTFVDIGQGDCIIIREPFNHRVMLIDTGGDLQIQKPIWARGHFSHDRSVGTSINYLKSLGIYQRDSVWLTHSDADHIGFLTTVMKNFRVKQILVPAGMETLPKFQTRLAYPVKVVPVTNQSSPMTDLQILHPLQVGSATNDDSLVLFGHFGKLRFLFMDDLPQNGEKLIMKQYPNLQADVLKLGHHGSKTSSNLAFLKQVQPHYGIISAGRRNRYHHPNLETMQRLQQSKIVPLSTQNYGMIRYRYYSFYDQLTTKLNGDEKFWISQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL52</accession>
    <name>H4GL52_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphopantetheine adenylyltransferase</fullName>
            <ecNumber evidence="6">2.7.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Dephospho-CoA pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Pantetheine-phosphate adenylyltransferase</fullName>
            <shortName evidence="6">PPAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">coaD</name>
        <name evidence="8" type="ORF">PS3_2763</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the bacterial CoaD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.3" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122784.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2763"/>
    <dbReference type="EnsemblBacteria" id="EHS84682">
        <property type="protein sequence ID" value="EHS84682"/>
        <property type="gene ID" value="PS3_2763"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1807"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00355"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02163">
        <property type="entry name" value="PPAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00151">
        <property type="entry name" value="PPAT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001980">
        <property type="entry name" value="PPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342:SF1">
        <property type="entry name" value="PTHR21342:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01020">
        <property type="entry name" value="LPSBIOSNTHSS"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01510">
        <property type="entry name" value="coaD_prev_kdtB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 6 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="7">
        <location>
            <begin position="5"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="9"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="88"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="123"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="177" mass="19993" checksum="D4AC9B6E260112EE" modified="2012-04-18" version="1">MKVALFPGSFDPLTMGHLDLIRRASHLFDQVAVAVMNNSSKHPLFTVQERVNQIETAVAGLSNVSVITAQGLTVNLMNQMGADYLLRGLRNETDFRYERDIAAMNHYLDDQIETVFLLTDPKYQHLSSSLLKEVALEGGDISAYLPENLNQALQKQIHDRMIERVKQFNEKSRTSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL69</accession>
    <name>H4GL69_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cell division initiation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_22302</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122801.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_22302"/>
    <dbReference type="EnsemblBacteria" id="EHS84699">
        <property type="protein sequence ID" value="EHS84699"/>
        <property type="gene ID" value="PS3_22302"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1831"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019933">
        <property type="entry name" value="DivIVA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007793">
        <property type="entry name" value="DivIVA_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35794">
        <property type="entry name" value="PTHR35794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05103">
        <property type="entry name" value="DivIVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03544">
        <property type="entry name" value="DivI1A_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 8" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="179"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="29"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="183"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="213"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="229" mass="25491" checksum="2FB00CFF50211A27" modified="2012-04-18" version="1">MGLTVDEITNKTFDNKMRGFNPDQVKEFLGQVAGQLRELEAQNHELAEKAKASEEKLRYLSDLKDSLNKSILVAQEAAEKVKNNAEREAEITVREAQKQATDIVTAANEKANAVIAECANSTRKLTVATSDLKKSTRIFRQRLQVMLESQLEVLHGEDWDQLLADDDYSKLDDLEKILGSRLDSDSSESVESTPTTQTTSESLEPLSEIPDSESETDDGKTVVVFPEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL96</accession>
    <name>H4GL96_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TGT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_20613</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122828.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20613"/>
    <dbReference type="EnsemblBacteria" id="EHS84595">
        <property type="protein sequence ID" value="EHS84595"/>
        <property type="gene ID" value="PS3_20613"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1872"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="SSF51713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGT" evidence="1">
        <location>
            <begin position="6"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="285" mass="32694" checksum="99FD230434816E4F" modified="2012-04-18" version="1">MTQSQLVPMLNQETISPLTAMEWQQLGIKTLAVTAVDNLRTEQHAKFTTIGGWAKFYQWSGQVIAWSGIQAPFKKINKNTIKSGATYWRQQQSQKLTLSDYEWWLAGVQADIVLPLAQAAPYYGPVDDIERAVEVNLAWQNHHLWGVIQGAGLKRARQRSVQGLIDQGVQNFVLGGNNQHLPFDEWQRINEAILAMLPDGRQTMMRVSSLAELQQAVSWKINYILTDLPRYWNRHRKVFWSGQLVDLSEVNDASTRLMQEETVLGERLLMSHNWQQLNNLLKNND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLA7</accession>
    <name>H4GLA7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutamine ABC transporter substrate binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_3014</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122839.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3014"/>
    <dbReference type="EnsemblBacteria" id="EHS84606">
        <property type="protein sequence ID" value="EHS84606"/>
        <property type="gene ID" value="PS3_3014"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1884"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003591920" evidence="2">
        <location>
            <begin position="31"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="3">
        <location>
            <begin position="44"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS84606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="284" mass="31769" checksum="D7DF9356B73982FE" modified="2012-04-18" version="1">MKRLIRIGLLVLLLMPVMLVAGCQTVTQRADTQDTWSQIKKRGKVVIGVDDSFVPMGFREINGKLVGYDVDLARAVFKQYGIKVDFQTIDWSMKETELRNGTIDAIWNGYSVTKERKAKVAFTRSYLLNRQVLVTKKKSHILNLNDMAGKVLGLQTGSTAAADLDAQPKLLKDKIKDQTPILYDTFPSAFIDLNAGRIQGILMDEVYANYYLKHLSNSGSYRTLISKAFPADSFAVGLRKGDKTLKKKINDGLAKLQKDGELAKINEKWFGKKSNYLGPVNANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLA9</accession>
    <name>H4GLA9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Amino acid ABC superfamily ATP binding cassette transporter, membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_3548</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122841.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3548"/>
    <dbReference type="EnsemblBacteria" id="EHS84608">
        <property type="protein sequence ID" value="EHS84608"/>
        <property type="gene ID" value="PS3_3548"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1886"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043429">
        <property type="entry name" value="ArtM/GltK/GlnP/TcyL/YhdX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614">
        <property type="entry name" value="PTHR30614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="15"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="213" mass="23827" checksum="6DC6764D91F7B2AC" modified="2012-04-18" version="1">MTYMSEILPSLLQGTVVTLEIFFWTLIGSLPLGILVSLGLNSRIKPLKWILDLYVWLMRGTPLLLQLIVVFYGLPIIGIVFPRFQAAVFAFIFNYGAYFAEIFRGGLQSIDRGQYEGAKVLRLSYSQTIRKIVIPQVVKIVIPSIGNEVINLVKDSSLVYVIGIGDLLRAGNVATSRDVTLVPLLMAGAIYLILVAICTFALNRVEKRYSYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLB4</accession>
    <name>H4GLB4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thiol peroxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_21680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122846.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21680"/>
    <dbReference type="EnsemblBacteria" id="EHS84613">
        <property type="protein sequence ID" value="EHS84613"/>
        <property type="gene ID" value="PS3_21680"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1892"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03014">
        <property type="entry name" value="PRX_Atyp2cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002065">
        <property type="entry name" value="TPX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="17"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS84613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="164" mass="18120" checksum="FF883FBC2FF5C93E" modified="2012-04-18" version="1">MQITINGEARDLVGNPPAVGEEIPHFKVFNANHEKVKTREFFDKPTLISVVPSITTSVCSLQTKKFNQQMDEYPNANFVTISVDTPEQQADWCAAEGVKNMQLLSDSEQSFGYALKVLIPDESVLARSIFVLDVDGKIVYEQVVPELTDEPDYAAALAALEKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHR2</accession>
    <name>H4GHR2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DedA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_10386</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the DedA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000003">
        <property type="protein sequence ID" value="EHS87557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10386"/>
    <dbReference type="EnsemblBacteria" id="EHS87557">
        <property type="protein sequence ID" value="EHS87557"/>
        <property type="gene ID" value="PS3_10386"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.25"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032818">
        <property type="entry name" value="DedA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30353">
        <property type="entry name" value="PTHR30353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="216" mass="24332" checksum="23791F3186BC2D0D" modified="2012-04-18" version="1">MSVINFFIDFVLHIDSHLVQIVTTFGDWTYLILFAIIFIETGAVILPFLPGDSLLFAASALSANALYHLNIWWFIIIFFIACFGGDSLNFYIGKNLEKLLARHTWFTKLIDQESLHKTEAFFEKHGAMAIILARFMPIIRTFAPFAAAGSGFPYRKFVRYSIIGCTAWVAICCGAGYFFGNLPFVKAHFSLIIIGIIVVSLIPALVSYLRSKLVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHR6</accession>
    <name>H4GHR6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PTS system mannose-specific EIIAB component</fullName>
            <ecNumber evidence="4">2.7.1.191</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">EIIAB-Man</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">EIII-Man</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PS3_11865</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II ManXYZ PTS system is involved in mannose transport.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-mannose(out) + N(pros)-phospho-L-histidyl-[protein] = D-mannose 6-phosphate(in) + L-histidyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:49232"/>
            <dbReference type="Rhea" id="RHEA-COMP:9745"/>
            <dbReference type="Rhea" id="RHEA-COMP:9746"/>
            <dbReference type="ChEBI" id="CHEBI:4208"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="ChEBI" id="CHEBI:64837"/>
            <dbReference type="EC" id="2.7.1.191"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.191" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000004">
        <property type="protein sequence ID" value="EHS87546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121600.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000004.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11865"/>
    <dbReference type="EnsemblBacteria" id="EHS87546">
        <property type="protein sequence ID" value="EHS87546"/>
        <property type="gene ID" value="PS3_11865"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.29"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00006">
        <property type="entry name" value="PTS_IIA_man"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00001">
        <property type="entry name" value="PTS_IIB_man"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.35.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004701">
        <property type="entry name" value="PTS_EIIA_man-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036662">
        <property type="entry name" value="PTS_EIIA_man-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033887">
        <property type="entry name" value="PTS_IIA_man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004720">
        <property type="entry name" value="PTS_IIB_sorbose-sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036667">
        <property type="entry name" value="PTS_IIB_sorbose-sp_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03610">
        <property type="entry name" value="EIIA-man"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03830">
        <property type="entry name" value="PTSIIB_sorb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52728">
        <property type="entry name" value="SSF52728"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53062">
        <property type="entry name" value="SSF53062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51096">
        <property type="entry name" value="PTS_EIIA_TYPE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51101">
        <property type="entry name" value="PTS_EIIB_TYPE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PTS EIIA type-4" evidence="19">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="PTS EIIB type-4" evidence="20">
        <location>
            <begin position="163"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="128"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EHS87546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="333" mass="36385" checksum="B6B7EF17E28C4748" modified="2012-04-18" version="1">MVGIVLASHGGFADGIFQSGEMIFGQQENVAHVILKPDEGPDDIRQKLSDAVATFDDQEQVLFLVDLWGGTPFNQVSNLFDEHKDTWAVVSGMNLPMVIEAYASRFSMNTAREIATHLVDVARDGIKTKPEDLMPAEETSTEAAPADNQPTGAIPEGTVLGDGHIKYVFARVDTRLLHGQVATGWTKAMNPDRIIVVSDNVAKDKLRKSMIQQAAPAGVHANTVPIKKMEEVAQDPRFGATKAMLLFETPEDALEAIKGGVELKELNIGSMAYTEGKVNVNKVLAMDQKDVDTLKELQEMGIKLDVRKVPSDSPESIDSLLKHAQELINEEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHT3</accession>
    <name>H4GHT3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Hemolysin III</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_12899</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0073 (Hly-III) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121617.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12899"/>
    <dbReference type="EnsemblBacteria" id="EHS87489">
        <property type="protein sequence ID" value="EHS87489"/>
        <property type="gene ID" value="PS3_12899"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.52"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019835">
        <property type="term" value="P:cytolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005744">
        <property type="entry name" value="Hy-lIII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="PTHR20855"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01065">
        <property type="entry name" value="hlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="215" mass="23995" checksum="081FB36C5EBEA9DF" modified="2012-04-18" version="1">MTITKRHYKTLLIEIGNAITHGLGAALAIAGLVVLIVHAALHPQVHNAMRITTFAIYGSIMVLFYLASTLFHSLIFTRAKHVFQVFDHSMIYMMIAGSYTPYCLVAIRGALGWTLFGIIWAMAIAGIVYKSIWLNKKSHLSTMIYVIMGWMCLIALVPLWHSLGPAGFGLLFAGGVVYTLGALLYSFPTDYTHFIWHFFVMAGSALMYFSILFYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHX9</accession>
    <name>H4GHX9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamine amidotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_2757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2757"/>
    <dbReference type="EnsemblBacteria" id="EHS87535">
        <property type="protein sequence ID" value="EHS87535"/>
        <property type="gene ID" value="PS3_2757"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.106"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01741">
        <property type="entry name" value="GATase1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044992">
        <property type="entry name" value="ChyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695">
        <property type="entry name" value="PTHR42695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GATase" evidence="1">
        <location>
            <begin position="41"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="231" mass="25830" checksum="2045B833827111FD" modified="2012-04-18" version="1">MTSKGVIKMRVNVLQHVSDEGPGSVMNWAHEHGAIVTVYHPNDFQKLPTVDETDLLVVLGCPASPNDSDQWIKDERKLIKELMDAHKPMLGMCFGAQQMAKILGAEVKDAPTKEVGWLEVTKQSDVIPDLPATVTPLHWHQQKFTIPEGGQLLYSSKYLREQAFIYQDYVIGLQFHLEPLIDNVREMVANDGDYADGSVLNQSGQEIIDHGVPAENQLVMNQLLDYLAKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHY0</accession>
    <name>H4GHY0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aspartate--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="8">AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">aspS</name>
        <name evidence="10" type="ORF">PS3_4557</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of L-aspartate to tRNA(Asp) in a two-step reaction: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp)</text>
            <dbReference type="Rhea" id="RHEA:19649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9678"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.12" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121664.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4557"/>
    <dbReference type="EnsemblBacteria" id="EHS87536">
        <property type="protein sequence ID" value="EHS87536"/>
        <property type="gene ID" value="PS3_4557"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.107"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00044">
        <property type="entry name" value="Asp_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004524">
        <property type="entry name" value="Asp-tRNA-ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF5">
        <property type="entry name" value="PTHR22594:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="SSF55261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00459">
        <property type="entry name" value="aspS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="9">
        <location>
            <begin position="140"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="8">
        <location>
            <begin position="197"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="219"/>
            <end position="221"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="489"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="534"/>
            <end position="537"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="593" mass="67234" checksum="018645AC22A2122B" modified="2012-04-18" version="1">MQRTNYAGLTDESQLGQRVVLKGWVAKRRNLGGLLFIDLRDREGIVQLVFNEQENEAAFESASHCRNEDVIEIAGTVRQRSEINPELATGKIEVVVETINVLAKSQTPPFEVVDNVDAAEDLRMRYRYIDLRRPEMMRNMKLRSKVTAVVHNYYDNHDFMDVETPDLTLSTPEGARDYIVPSRVYPGHFYALPQSPQLFKQLLMAAGIDKYYQIARCFRDEDLRGDRQPEFTQIDTEMSFATPEEIQTVAEGLIKRVMKEVLDIEVQTPFPRMEWQEAMDRYGSDKPDTRFGMEIHDLSDLVADSDFKVFASTVSAGNYVRAICVPGGADAYSRKDITKKEEFIKRYHAKGLAWVKVTDDGYNGPIAKFINDLAAPINQRLGAKTGDLILFVADSFNIVCNALGYLREAIAQELDLIAPDQWNYLWVVNWPMFEYDEGFDRWIAAHHPFTMLNEEDLHYLEPGEDPHKAHAQSYDIVLNGNEIGGGSMRIHNPEVQEKVLKALGYSKEDAQARFGFLLNALENGMPPEGGMAFGLDRWVMLLAQAESIRDVIPFPKNSKAVEPLTGAPGQVELNQLEDLHLKVDLEGSNQDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHY4</accession>
    <name>H4GHY4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA small subunit methyltransferase E</fullName>
            <ecNumber evidence="6">2.1.1.193</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_17941</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) + N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42920"/>
            <dbReference type="Rhea" id="RHEA-COMP:10283"/>
            <dbReference type="Rhea" id="RHEA-COMP:10284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74502"/>
            <dbReference type="EC" id="2.1.1.193"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the RNA methyltransferase RsmE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.193" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121668.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17941"/>
    <dbReference type="EnsemblBacteria" id="EHS87540">
        <property type="protein sequence ID" value="EHS87540"/>
        <property type="gene ID" value="PS3_17941"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.112"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006700">
        <property type="entry name" value="rRNA_ssu_MeTrfase-E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30027">
        <property type="entry name" value="PTHR30027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04452">
        <property type="entry name" value="Methyltrans_RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015601">
        <property type="entry name" value="MTase_slr0722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00046">
        <property type="entry name" value="TIGR00046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="247" mass="27657" checksum="F1C617AC62AA8F24" modified="2012-04-18" version="1">MQRYFISTPTTEGIIQLTPDTCHHLIDVMRAQVGTQFELVVNHQNVYLAELVDADRGTAKLLETIPNEVELPLKVTLACGLPKTKEKPEMIVQKATELGAHQIIFFEAQRSVSHWTSGKEVKKLARLQKIANAAAEQAHRIIQPRVTYVKKLVDVFGQQDFDIRLVAWEESAKQGETKALVKNLQKAQAGQEILAIFGPEGGLTQQEIDQLATYQVEPVGLGPRILRTETAPLYLMSAISYQIELFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHZ9</accession>
    <name>H4GHZ9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Lysyl-tRNA synthetase</fullName>
            <shortName evidence="8">LysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">lysS</name>
        <name evidence="11" type="ORF">PS3_10579</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8 9">Binds 3 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000007">
        <property type="protein sequence ID" value="EHS87450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121683.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000007.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10579"/>
    <dbReference type="EnsemblBacteria" id="EHS87450">
        <property type="protein sequence ID" value="EHS87450"/>
        <property type="gene ID" value="PS3_10579"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.129"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00775">
        <property type="entry name" value="LysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04322">
        <property type="entry name" value="LysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00252">
        <property type="entry name" value="Lys_tRNA_synth_class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002313">
        <property type="entry name" value="Lys-tRNA-ligase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044136">
        <property type="entry name" value="Lys-tRNA-ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00499">
        <property type="entry name" value="lysS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="10">
        <location>
            <begin position="179"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="496" mass="57419" checksum="F931EA4B574CB3EF" modified="2012-04-18" version="1">MAEEHLNDQMQVRRDKMQSLRERGIDPFGQRFAPDHSTSNEVREKYGEDDGELLLQQGNVVTIAGRLIGKRGSGKASFADLLDRDGKIQIYVRQDRVGEESYQLFKESDLGDFWGITGDVIKTRMGELTVRANQITFLTKALRPLPDKYHGLQDPELIYRQRYLDLITNQESLERFKNRTKIIKAIRRYMDENDFMEVETPMLETVASGADARPFETHHNALNIEMYLRIATELRLKRLIVGGLERVYELGRVFRNEGMDPRHNPEFDTMETYAAYFDFNDVMAETEGIFKAAAKVMNHDMVINYQGTELDFSKPFTRMHMVDAIKETTGIDFWEPMTDDQARQLADDNGVKYESWWKVGHIINAFFEEKVQDQLEQPVFIYGHPVEISPLARKNKDDERFTDRAELYILGSEYANMFTELNDPIDQRARFEAQAQEHEAGNDEFQPIDEDFVEALEYGMPPTGGLGVGIDRLVMLMTDAKSIRDVLLFPTMRLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI24</accession>
    <name>H4GI24_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphate transport system permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PS3_14260</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000011">
        <property type="protein sequence ID" value="EHS87434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121708.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000011.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14260"/>
    <dbReference type="EnsemblBacteria" id="EHS87434">
        <property type="protein sequence ID" value="EHS87434"/>
        <property type="gene ID" value="PS3_14260"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.163"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011864">
        <property type="entry name" value="Phosphate_PstC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02138">
        <property type="entry name" value="phosphate_pstC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="78"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="297" mass="31802" checksum="B1FEAD8A524F8F58" modified="2012-04-18" version="1">MTKHKLLQPSKESRQDRHGKCWVGLAIAIIGLLVITILIFLCTKGVVLFVNHGIGVFDFMTGVKWSPSEEKFGALPMILTSFVVTLVAGLIALPLALLVALAITEILPERFQNLLRSLVELMVGIPSVIYGFIGLTVVVPQLRQWFGGTGFGLLAAIVVLVMMIFLTITSMTVDTLRATPAAYRIGSMGLGATRWQTIWHVILPYAKGGIITALIFGMARAFGEALAVQMVIGNTVSLPNGWMNPAATLTSVITTGIGNSVMGTPANDGLWSLALVLLLMSLIFNGLAQLTKGRAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI30</accession>
    <name>H4GI30_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Teichoic acid D-alanyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_8078</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">O-acyltransferase that catalyzes D-alanylation of both teichoic acid and lipoteichoic acid (LTA). D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall. Catalyzes D-alanylation from DltC carrier protein.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cell wall biogenesis; lipoteichoic acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the membrane-bound acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000011">
        <property type="protein sequence ID" value="EHS87440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121714.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000011.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8078"/>
    <dbReference type="EnsemblBacteria" id="EHS87440">
        <property type="protein sequence ID" value="EHS87440"/>
        <property type="gene ID" value="PS3_8078"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.170"/>
    <dbReference type="UniPathway" id="UPA00556"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070395">
        <property type="term" value="P:lipoteichoic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024194">
        <property type="entry name" value="Ac/AlaTfrase_AlgI/DltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024024">
        <property type="entry name" value="DltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016636">
        <property type="entry name" value="AlgI_DltB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500216">
        <property type="entry name" value="DltB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04091">
        <property type="entry name" value="LTA_dltB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="374"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR016636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="406" mass="48414" checksum="6A434AC594D47513" modified="2012-04-18" version="1">MLNWLQTLPNYSAYGSPTYFIYLIIAIIPLMIGIYFGKRLVWYEAFVSFLFIFFMFTGSSVQQGCSLIAYIIYETILVMWYFYYRQKHNTSWVFYLLAFLSILPLVIVKVTPAMVGHNSLLGFMGISYLTFRAVGTIIETRDGVLKELNWWKFIRFMVFMPTITSGPIDRYRRFATDYQAVPERQEYLKMVDKAIYYLFLGFLYKFVITYFFAMQWQPFMEKMAMLSAPHLSWWVVGVAYTYAGNLFFDFAGYSLFAVGISYLMGVKTPMNFNKPFSSPNIKEFWNRWHMTLSFWFRDYIFMRFVFLATKKRWFKSRNALSSTAYMLNMIIMGFWHGVTWYYILYGFLHGLALVVNDAWLRYKQKHFKNLKSTWLTKGIAILITFNFVVFTFLIFSGFLNKLWFTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI32</accession>
    <name>H4GI32_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein DltD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_12865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; lipoteichoic acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DltD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000011">
        <property type="protein sequence ID" value="EHS87442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121716.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000011.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12865"/>
    <dbReference type="EnsemblBacteria" id="EHS87442">
        <property type="protein sequence ID" value="EHS87442"/>
        <property type="gene ID" value="PS3_12865"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.172"/>
    <dbReference type="UniPathway" id="UPA00556"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070395">
        <property type="term" value="P:lipoteichoic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006998">
        <property type="entry name" value="DltD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023896">
        <property type="entry name" value="LTA_DltD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40039">
        <property type="entry name" value="PTHR40039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04914">
        <property type="entry name" value="DltD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021438">
        <property type="entry name" value="DltD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04092">
        <property type="entry name" value="LTA_DltD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR021438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="431" mass="50084" checksum="2FA554EDFFE9954A" modified="2012-04-18" version="1">MKKPAKQLWLVLGPLFVAALLCLLVFAYPWSGKRSQATLQRAAVSLSNVVFKNRDLKVQALSDKSKHYVAFFGSSELNRMDKYHPSVMAAKYHNYTPFLFGSRGTQSLPQMLNMTMMSKQLTNKKAVFIISPQWFTKPGVDKMAFAYYTGSYSDIYWLLQANPKSAYDRYTAQRLIELLGTDGTIPNAAKRIASGKVLTGFERRWLSFRMSLLAHEDNLFSGYFLNNNYQKKIVPDEEALPVTYNYSKLYQQAAKDKAKQSKNNHLGIIDSFYRKQLRGKYWSTKNSQRHFDYYKSPEYGDFETVLKQFQKTNTNVIFVITPVNEKWEKHTGMSMKMYYQTVNKIKFQLQSQGFNNIVDFSHKGNQSSFMNDTIHLGWSGWVDFDRQVSRFIENKQASPNYHVNDVFLSKKWTNLIPTTNNLKQFQKTELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI36</accession>
    <name>H4GI36_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Dipeptide/tripeptide transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_3355</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000012">
        <property type="protein sequence ID" value="EHS87428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121720.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000012.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3355"/>
    <dbReference type="EnsemblBacteria" id="EHS87428">
        <property type="protein sequence ID" value="EHS87428"/>
        <property type="gene ID" value="PS3_3355"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.178"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006857">
        <property type="term" value="P:oligopeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17346">
        <property type="entry name" value="MFS_DtpA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005279">
        <property type="entry name" value="Dipep/tripep_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="PTHR11654"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00924">
        <property type="entry name" value="yjdL_sub1_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01022">
        <property type="entry name" value="PTR2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="404"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="440"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="467"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="499" mass="54733" checksum="4EDD7D209634EC07" modified="2012-04-18" version="1">MGKSRNLDTAFFGQPRGLSTLFFTETWERFSYYGMRAILLYYMYYAVSQGGLGMDQATAASLMSIYGSLVYLSSIVGGWLSDRVFGSYWTVFYGALLIMFGHISLSLPFGQGALYASITFIVVGSGLMKPNITNMVGKLYSEEDARRDAGFSLYYFGVNFGAFIAPIAVPWAAGGFGFHAFGAHQNFHAGFSLAAIGMFIGLVQYLIDGKKYISPQSLVPTEPIEKENLRRVVTWIVAGLVVVLAFLGGLFLVGQLNIENIINMVTVLAFIVPIAYFVIMLKSKEVTSKEKKKVQAYIPLWIAAVAFWSIEESGSVVLALFAAQRTVLHIGGWHYSAANFQVLNPLFIMILTPIFVWLWNNWKNQPSSPKKFSGGLIFAGASYIWMALPGIIHGTTAGKVSPFWLVGSWFICMIGEMLLSPIGLSVTARLAPKAYSSQMMSLWLLADAAAQAINAQIVKFYSTGTEVPYFLSIGAVSVVLGIGLLLISKKIFSLMEDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI50</accession>
    <name>H4GI50_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="14">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phenylalanyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="14">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">pheT</name>
        <name evidence="19" type="ORF">PS3_9429</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 14">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="14"/>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121734.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9429"/>
    <dbReference type="EnsemblBacteria" id="EHS87389">
        <property type="protein sequence ID" value="EHS87389"/>
        <property type="gene ID" value="PS3_9429"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.195"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02796">
        <property type="entry name" value="tRNA_bind_bactPheRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00283">
        <property type="entry name" value="Phe_tRNA_synth_beta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004532">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033714">
        <property type="entry name" value="tRNA_bind_bactPheRS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PTHR10947"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03147">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00896">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="SSF54991"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00472">
        <property type="entry name" value="pheT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51447">
        <property type="entry name" value="FDX_ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 14 19" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 14" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="16">
        <location>
            <begin position="42"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="B5" evidence="18">
        <location>
            <begin position="412"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="FDX-ACB" evidence="17">
        <location>
            <begin position="717"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="476"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EHS87389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="810" mass="90024" checksum="7D91BB6278600D31" modified="2012-04-18" version="1">MNLSYQWLNEYLPLADHQIEPAELAEKLARTSVEIDDVYSPSTGLSKLVVGYIESCQPHPESDHLKLCQVQISSEEKVQIVCGAPNVTAGKKVIVALPGARVADNQKIRKGKIRGEVSNGMLCALQEIGFSEKVAPKDYEEGIWFMADDAQVGDSVFPYLGMDDTIIATDVTPNRGDMFSVYGNVNDIAAMYDLPVNFSMHTVEESSAEKTTDLLQVEIPDPELAPTYKARVIKNVTIQDSPMWLQIRLWNAGVRPINNVVDVTNYILLKYGQPLHSFDFDKLNQKHLTVRHAQADEHLVTLDGEDRELTSQDLVVADGDHPVALAGTMGGDDSKIDDQTINVVLESAIFDPILVRKQARCLDLHSESSMRFERGINPAMCEQALNEAAYWLQELAGGQATQGIATGSEKVARDVNIDLSLTKTNDVLGTDLSMTDLQQIFKQLQFTTQVMNDDHLEVVVPPRRWDIEIQADLYEEIARIYGYDNLPSTLPATGDTEGGLTAWQKLVREARHVLEGLGLNQAISYSLTTDEKARQFKINPSETAPVQLDYPMSSDHTAARMNIISGLLNDVAYNVARQVENVALYEEGRVFLPQADQARPDEPEHIAMALTGSLTTNSWLDKAQPVDFYTVKGILEQLLTNLAIAGKVSYRANDQRDEMHPGRTADVYLDDQLIGFLGQVHPVTAQAYKIPETYVMELDLSQLETAPRLDHHYEPISKYPTVTRDVALLVDQTVTNAQIVEAIQAKGGQYLVDVQLFDLYEGAHLPAGKKSLAYTLTYQDRQNTLTEDQVNAAFEKVVDHLQTTLAAEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI59</accession>
    <name>H4GI59_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_6529</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121743.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6529"/>
    <dbReference type="EnsemblBacteria" id="EHS87398">
        <property type="protein sequence ID" value="EHS87398"/>
        <property type="gene ID" value="PS3_6529"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.211"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="7">
        <location>
            <begin position="130"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="130"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="228" mass="26352" checksum="59A6C66FCE2CE132" modified="2012-04-18" version="1">MSRVLIIEDEENLAHFVELELKHEGYETKVALDGREGLEAALADDYDVILLDLMLPELNGLEVARRVREVKNTPIIIMTARDSVIDRVSGLDHGADDYIVKPFAIEELLARVRALLRRISIEEDNASDHQTKVTYKDLVIEKENRIVRRDEEVINLTKREYELLLILMENINVVMSRKDLLSKVWGYDTNVETNVVDVYIRYLRNKIDRPGEPSYIQTVRGTGYVIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI82</accession>
    <name>H4GI82_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_4598</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121766.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4598"/>
    <dbReference type="EnsemblBacteria" id="EHS87421">
        <property type="protein sequence ID" value="EHS87421"/>
        <property type="gene ID" value="PS3_4598"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.238"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="245" mass="27145" checksum="529A140B401F2F1B" modified="2012-04-18" version="1">MLTIDQLVGGYSQVPVLKNISFQVQPGELVGLIGLNGAGKSTTINHIIGLLQPFSGLIQVNGATLANDPLTYKKAIAYVPETPVLYEELTLREHLQLTINAYQLAPTSAWQRAKELLKTFRLENKLDWFPVDFSKGMKQKVMICMAFMTEAKLLVIDEPFYGLDPLAVHDLLTIIDQKKAQGVAILMSTHVLDTAQRYCDRFVLLADGQVQAQGSLQDLQALNGHQGESLDEIYLQLAQGDRHAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIA4</accession>
    <name>H4GIA4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Argininosuccinate synthase</fullName>
            <ecNumber evidence="2 9">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">argG</name>
        <name evidence="10" type="ORF">PS3_13645</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + L-aspartate + L-citrulline = 2-(N(omega)-L-arginino)succinate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the argininosuccinate synthase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="2 9"/>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121788.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13645"/>
    <dbReference type="EnsemblBacteria" id="EHS87325">
        <property type="protein sequence ID" value="EHS87325"/>
        <property type="gene ID" value="PS3_13645"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.269"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01999">
        <property type="entry name" value="Argininosuccinate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00005">
        <property type="entry name" value="Arg_succ_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001518">
        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018223">
        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023434">
        <property type="entry name" value="Arginosuc_synth_type_1_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024074">
        <property type="entry name" value="AS_cat/multimer_dom_body"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="PTHR11587"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="SSF69864"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="9"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="403" mass="44416" checksum="0DA0EDCA076394BF" modified="2012-04-18" version="1">MANPKVVLAYSGGLDTSVEIAWLKNAGYDVIAVCFNVGEEEMDLSAIKQKGLDVGAIESIVVDARHEFATEYVLPALQANALYEGVYPLVSALSRPLISKHLVEIAKEYGAVAIAHGCTGKGNDQVRFEVSIHALAPDMIIEDPIREVHWSREEEIAYAKDNNIPVPITKESPYSIDENLWGRANEAGVLEDPWVGAPEDAFARTNALENTPDTPAVVELSFVQGIPTALNGRELPLEELIAQLDHLAGQHGIGRIDHIENRLVGIKSREVYEAPAAEVLIKAHQDLENLTMERGLGHYKPLVEHEISNLIYNAQWFSPLFNSLMAFIKASQTDVTGTVRVKLFKGNVICEGRKSPLSLYNEDLATYTSSDSFDHESANGFIKLWGLPTQVYAQVHQHAEQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIC8</accession>
    <name>H4GIC8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphatase YbjI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_15024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121812.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15024"/>
    <dbReference type="EnsemblBacteria" id="EHS87349">
        <property type="protein sequence ID" value="EHS87349"/>
        <property type="gene ID" value="PS3_15024"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.309"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000150">
        <property type="entry name" value="Cof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00099">
        <property type="entry name" value="Cof-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="266" mass="29509" checksum="97160055ACC274F4" modified="2012-04-18" version="1">MIKAIAVDMDGTFLHSDGTYNHDRFLKVFRQLQDHQITLIAASGNPHYQLKKQFAPVQADLAYVAENGVEVIQAGQVLSCEQMTSSAIQNVFQLHQDLPNSQFILSGFNQAYLMGEKDDEFFDSVKHHYLGLKRIDDLQEVNEPIVKMQIKVPVDQTSQLQTYINDHIQGVTAVTSGYDDLDLIITGQDKAAGLAKLCQANHWQASELMAFGDGQNDLSMLNFVGHSYAMANGMAIVKEAAQNLAPSNDEDGVLQVIEAYLIQQYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIC9</accession>
    <name>H4GIC9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_7736</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121813.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7736"/>
    <dbReference type="EnsemblBacteria" id="EHS87350">
        <property type="protein sequence ID" value="EHS87350"/>
        <property type="gene ID" value="PS3_7736"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.310"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028978">
        <property type="entry name" value="Chorismate_lyase_/UTRA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011663">
        <property type="entry name" value="UTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07702">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00866">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64288">
        <property type="entry name" value="SSF64288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="242" mass="27690" checksum="09FDF9A3B23D1C89" modified="2012-04-18" version="1">MVDLVYRTVMKDIKQKIMHGEYPEMKLPDERSLSQQYAVSRSSIKRALEILVRQGIIFKKRGSGTFINPLYLKKQAMFSYEGSNLGITDSYQFDGKTQRIEVKSFQVIPASEEVQTDLFLKPTDFVYEIKRIRYIDDQPFILETGYIPIKIAPTISEEVVQGSVFNYIEAALGKTVTRSYVSVSAAPSTAVDQELLDLKPTEPVAVLDGVFFLDDGTPFEVSNMRFHYRYLRYNTFTNINGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GID0</accession>
    <name>H4GID0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase</fullName>
            <ecNumber evidence="3">4.2.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_3337</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the prolyl-tRNA editing family. YbaK/EbsC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.-.-" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121814.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3337"/>
    <dbReference type="EnsemblBacteria" id="EHS87351">
        <property type="protein sequence ID" value="EHS87351"/>
        <property type="gene ID" value="PS3_3337"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.311"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00002">
        <property type="entry name" value="YbaK_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004369">
        <property type="entry name" value="Prolyl-tRNA_editing_YbaK/EbsC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007214">
        <property type="entry name" value="YbaK/aa-tRNA-synth-assoc-dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04073">
        <property type="entry name" value="tRNA_edit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006181">
        <property type="entry name" value="EbsC_YbaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="SSF55826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA_edit" evidence="4">
        <location>
            <begin position="45"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR006181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="165" mass="18256" checksum="FD8DF6174F534242" modified="2012-04-18" version="1">MSKKKGIKKTQVEQILDKHHIPYQQAAFATTQDGDSARSLKMYHQGINDHLIYKTLVLRGNVTGPLVGVVPIDRHLDEKALARVSGNKKVEMVPLKDLLKTTGYVHGANTPIGIYEKYHYPIFIDNEAQATPTIYVSSGQVGRSVQLKASDLADLVDATFADLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIE7</accession>
    <name>H4GIE7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MFS family major facilitator transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_14053</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121831.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14053"/>
    <dbReference type="EnsemblBacteria" id="EHS87368">
        <property type="protein sequence ID" value="EHS87368"/>
        <property type="gene ID" value="PS3_14053"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.343"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="351"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="394"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="438"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="12"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="464" mass="50961" checksum="20B33B9E8DA87898" modified="2012-04-18" version="1">MTETTSKTTYWALFSTALITFLGILNETSMNVTYQILCNEFHISLDMVQWITAGYLLIVTIVMGTTAYLIRKYAARWLHLTAGIAFIIGCLLCALAPNFPVLLVGRLIQGIATGFSTPLMFHLIFTQIPKNKLGVMSGIAGMVISFAPALGPTFGGAVATSLSWRWIFWIIIPFVILGTVIGQLTIRNHPMGNDKPFSYLGLFVLGLSLFAFVYSTAQLGSHGFNQDFWIWCLLGILFLVVFIGVNHFGKAELINLRIFKNGSISLATITYFCLQFINIGLSVIIPTYAIYVLHSSSFIAGLILLPGSFAGAFTSPFAGTLADRIGFRLPISIGSYLLIIGTWFYMGLQSLLTPLMISLVYIVIRVGFNLAFANTISNASTVVSRQETADVNSVFNMVQQFAGSISVSLSAALISIQQRSKPTSAIAFHTYLGGRQDFIMMFVLAVIIAITVWYNFHLQAKTKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIF0</accession>
    <name>H4GIF0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Galactose-1-phosphate uridylyltransferase</fullName>
            <shortName evidence="10">Gal-1-P uridylyltransferase</shortName>
            <ecNumber evidence="10">2.7.7.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">UDP-glucose--hexose-1-phosphate uridylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">galT</name>
        <name evidence="13" type="ORF">PS3_7278</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>alpha-D-galactose 1-phosphate + UDP-alpha-D-glucose = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:13989"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.7.7.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the galactose-1-phosphate uridylyltransferase type 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.12" evidence="10"/>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121834.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7278"/>
    <dbReference type="EnsemblBacteria" id="EHS87371">
        <property type="protein sequence ID" value="EHS87371"/>
        <property type="gene ID" value="PS3_7278"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.346"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008108">
        <property type="term" value="F:UDP-glucose:hexose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00571">
        <property type="entry name" value="GalP_UDP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000766">
        <property type="entry name" value="GalP_uridyl_Trfase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023425">
        <property type="entry name" value="GalP_uridyl_Trfase_II_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005850">
        <property type="entry name" value="GalP_Utransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005849">
        <property type="entry name" value="GalP_Utransf_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39191">
        <property type="entry name" value="PTHR39191"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02744">
        <property type="entry name" value="GalP_UDP_tr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01087">
        <property type="entry name" value="GalP_UDP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006005">
        <property type="entry name" value="GalT_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01239">
        <property type="entry name" value="galT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01163">
        <property type="entry name" value="GAL_P_UDP_TRANSF_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="7 10 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GalP_UDP_transf" evidence="11">
        <location>
            <begin position="17"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="GalP_UDP_tr_C" evidence="12">
        <location>
            <begin position="236"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS87371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="482" mass="54639" checksum="7E66E698BAA52386" modified="2012-04-18" version="1">MGVISQYADQVIASGAYEPLDRNYVMNKIRGLVGDEDVAVGDGRLVDQLVELAIAHGAIEDSVTDRSILNDQLFDLMTPSPARVNARFWEDYHRSPQEATDHFYQLCTENDYVKVAAIKKNIEFVKKVQDGNELEITINLSKPEKDPKAIQAAAHDTAVKYPQCALCMENEGYKGRLGYASRSNHRIIRLTVGGREWGFQYSPYAYFNEHCIFLLAQHEPMEINQQTLVNLVEIEQTFPHYFVGSNADLPIVGGSMLAHEHYQGGRHTFPMMKAPIKQELTFPDYPMVTAGIVDWPMSDLRLTSTDTTALVNLGSHIMRTWQNYSDPSLQINAGTADDRHHTVTPIMHRDGDQFILDLVLRDNNTTNQYPLGIFHPHEKLWHIKKENIGLIEVMGRAILPARLKDELAEVKKYWLQEPNKIAESHLPWAQEVQARQTITADNVETVLEQEVAQVFSDVLADAGVFKRNADGEAGWQRFIKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIF4</accession>
    <name>H4GIF4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_20649</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121838.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20649"/>
    <dbReference type="EnsemblBacteria" id="EHS87375">
        <property type="protein sequence ID" value="EHS87375"/>
        <property type="gene ID" value="PS3_20649"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.351"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="107" mass="11887" checksum="29CDD1A13F6A2A71" modified="2012-04-18" version="1">MSIEIPTNMEEVAMKVAQQQVRGNNITVDQVIQTAIQETMQALLDNAFDGHYDEVKWQDQSLVVIDFMGELVGQATSETSLVDEFKSNADQLTDRLAQATEQIVGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIH6</accession>
    <name>H4GIH6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UDP-N-acetylmuramyl-tripeptide synthetase</fullName>
            <ecNumber evidence="3">6.3.2.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">UDP-MurNAc-tripeptide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">murE</name>
        <name evidence="7" type="ORF">PS3_10766</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="3">Carboxylation is probably crucial for Mg(2+) binding and, consequently, for the gamma-phosphate positioning of ATP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the MurCDEF family. MurE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.-" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121860.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000017.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10766"/>
    <dbReference type="EnsemblBacteria" id="EHS87297">
        <property type="protein sequence ID" value="EHS87297"/>
        <property type="gene ID" value="PS3_10766"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.377"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00208">
        <property type="entry name" value="MurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005761">
        <property type="entry name" value="UDP-N-AcMur-Glu-dNH2Pim_ligase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01085">
        <property type="entry name" value="murE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 4" id="KW-0133">Cell shape</keyword>
    <keyword evidence="3 4" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="6">
        <location>
            <begin position="128"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="5">
        <location>
            <begin position="346"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-MurNAc-L-Ala-D-Glu binding" evidence="3">
        <location>
            <begin position="173"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="129"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="3">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="506" mass="56704" checksum="8BD5DE4D69B2BFBF" modified="2012-04-18" version="1">MLTVNEVVRLLKERQLFEEVICDNQRHYTLPESVRATEITDVTYDSRQVKSQALFFCKGLNFNFKYLQDAVAAGATVTITERSQVDDILKTQLPVIAIIVTDVQKAMATVARAFYGNPDQALTMIGFTGTKGKTTSVYFTRHILQAVFGKQVAQLSSIDECLDGEHFIEAHLTTPESLDLFRMLKAAVDHGMKYLVMEVSSQAYKKSRVYGLEFDYGVFLNISPDHISPVEHPDFDDYLYCKSQIVANSKTVIISREIKDYTFLAEKAQAYQTKVISIGQDATADYQYQGHSHGQFTVKTSSGTSDYQIKIPGDFNYANATAAIAVAQQLTDDQEQIKAGLLATTVPGRMECLSNHHGYVAYVDYAHNYLSLSESFKFMKHEYPKGRLIVVIGAAGGKAESRRHDIGMTLSEYADVAILTSEDNFKEDPYQIVDDIKANITNPELQVIVEVDRVKAIEQAFAMVKPGDVIFMAGKGREVFMHDVDGDKPYIGDYQLSQKLMVEYDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GII1</accession>
    <name>H4GII1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative MDR transporter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_9927</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the major facilitator superfamily. EmrB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121865.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000017.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87302">
        <property type="protein sequence ID" value="EHS87302"/>
        <property type="gene ID" value="PS3_9927"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.382"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="354"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="400"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="538"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="9">
        <location>
            <begin position="10"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="563" mass="61840" checksum="F3E88550CAE92EE7" modified="2012-04-18" version="1">MSKSYKIWIILMANCIGIFLCMLDTTVMNIALPDIQTSLKVTLNSLSWPLNIYTIIFAALTIPLGKLAENIGIKHTYLIGLVVFTIGSVISSLSTSLIHLMIGRGIQSLGASIVFPLSMTIGINLVSLESRKKTIAALGITQGLAAALGPSIGGVLTQFLGWRWIFIINIPLTILSIILCLISFDLKNDKRTKESIDFLGALLCITMLSTLVLSLVQGRSWGWLSIPIIASLIISFVSVICFVTWEIHTSNPMIPMDLFKNHEFTGSAIAIVLSNLFLVAVTVVLPTYFVRIQSKSELTAALLITPITGMIFIFSPLAALIIDKLGARMVIAFGFILITISYVLFTNINMNNILLVVITCIILGSGYGIIAGPITVLAASDFEGSLLTASQSVAGVLRQVGISLAVAIYVTSLYANMVTAKSNSTNYINEQVAKLDISQSKKQAIINRSVNSLGKNSSKISTNHFSKKDRQQFINLAYQQELAKYPDLTDQTQKSNIYDKVKTTVETTLDNLNHKINQVITRIKNYARHQYELAFIKLYKYSIPFAALSILSFLFFPKKKTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIL7</accession>
    <name>H4GIL7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-oxoacyl-[acyl-carrier-protein] synthase 3</fullName>
            <ecNumber evidence="9">2.3.1.180</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">3-oxoacyl-[acyl-carrier-protein] synthase III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Beta-ketoacyl-ACP synthase III</fullName>
            <shortName evidence="9">KAS III</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fabH</name>
        <name evidence="12" type="ORF">PS3_15235</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>acetyl-CoA + H(+) + malonyl-[ACP] = 3-oxobutanoyl-[ACP] + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:12080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9625"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78450"/>
            <dbReference type="EC" id="2.3.1.180"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the thiolase-like superfamily. FabH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.180" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121901.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15235"/>
    <dbReference type="EnsemblBacteria" id="EHS87212">
        <property type="protein sequence ID" value="EHS87212"/>
        <property type="gene ID" value="PS3_15235"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.428"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033818">
        <property type="term" value="F:beta-ketoacyl-acyl-carrier-protein synthase III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01815">
        <property type="entry name" value="FabH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013751">
        <property type="entry name" value="ACP_syn_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004655">
        <property type="entry name" value="FabH_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08545">
        <property type="entry name" value="ACP_syn_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00747">
        <property type="entry name" value="fabH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACP_syn_III" evidence="11">
        <location>
            <begin position="106"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="ACP_syn_III_C" evidence="10">
        <location>
            <begin position="236"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="ACP-binding" evidence="9">
        <location>
            <begin position="252"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="324" mass="34943" checksum="6C860F0A4DB6F1B3" modified="2012-04-18" version="1">MEAIKIIETARYAPSKIVTNDDLSQIMDTSDEWITERTGIRQRHISEGENTSDLAIKVGERLLAKSGYQPDQVDLIIVATMSPDAYTPATAAIVQGELGASHAVAFDISAACSGFMYAMMVAKQLLQMPTVNRAMVIGAEVLSKIIDWQDRTTAVLFGDGAGGVLLEKDATATSIVLGQNWQTFGDQHDALSAGYTKVANQFPGPVTDLKPFYMDGHGVYRFATHQVPKSIIAAADQASIDVDAIDHFILHQANARIIRSVAKRLHVSQEKFPININEYGNTSAASEPILLSECVDQGIVKRGDIIALSGFGGGLTVGTMIIKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIN4</accession>
    <name>H4GIN4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="5 10">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PS3_4918</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Genetic information processing; DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="5 10"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4918"/>
    <dbReference type="EnsemblBacteria" id="EHS87229">
        <property type="protein sequence ID" value="EHS87229"/>
        <property type="gene ID" value="PS3_4918"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.448"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026459">
        <property type="entry name" value="RNR_1b_NrdE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013554">
        <property type="entry name" value="RNR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="PTHR11573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08343">
        <property type="entry name" value="RNR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="SSF48168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04170">
        <property type="entry name" value="RNR_1b_NrdE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00089">
        <property type="entry name" value="RIBORED_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RIBORED_LARGE" evidence="12">
        <location>
            <begin position="553"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="322"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS87229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="716" mass="81286" checksum="21376A24DA831757" modified="2012-04-18" version="1">MTQVKYFDLNNEINIPKNGQIQLEKDHEALIDFIETNVKPNTKQFASVAERLDWLIDNDYIEAGFIREYPIEFIEKLYDYLKAQDFHFKSFMAAYKFYAQYALRTNDKEYYLENYIDRVAMNALFLANGNQALAQDLADELIHQRYQPATPTFLNAGRKRRGEFVSCFEIQATDDMNSIGRTVNSALQLSKIGGGVGISLNNLREAGAPIKKIKGAASGVVPVMKLLEDSFTYSNQLGQRQGAGVVYLSVFHPDIIAFLAAKKENADEKIRIKTLSLGITVPDKFYELVENDEEMFLFSPYDVERVYSVPFSYVDITAEYDNMVANDEITKYKINARDLEDEISKLQQESGYPYIVNIDMENRENPIDGKIIMSNLCSEIAQVQSPSIINNDQSFAKLGTDVSCNLGSTNVANLMETPDFGRSVRAMTYGLTRISDSESLDIVPSIQKGNDELHAIGLGAMGLHGYLAKNHIQYGSPVAIEFTSVYFMLLNYWTLVASHEIAKERQTAFYNFEKSTYADGSYFDKYVTKDWGPQSEAVKNLFNGIFIPGIEDWQALKADIMKDGIYNAYRLAVAPNGSTSYIGDSTSSLHPIINRIEERQEKMIGKIYYPAPYLNNDTIKYYKSAYDTDMRSVIDTYAAAQQHVDQALSMTLFMRSSIPEGLYEWKNGRTDKMTTRDLNILRHYAYQSGIKSIYYIRTFTDDEDEIGANQCESCVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIQ1</accession>
    <name>H4GIQ1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_5750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121935.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87246">
        <property type="protein sequence ID" value="EHS87246"/>
        <property type="gene ID" value="PS3_5750"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.471"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003591860" evidence="2">
        <location>
            <begin position="32"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="230" mass="23560" checksum="DA767B66EF608272" modified="2012-04-18" version="1">MKSNGIKKGLVGTAALLAAVTLAACGTNKSASSESNTSNSSVTHTSAYDKATKQIAAGKYQAALDTLNDSGSTSTKTKNLASDLQNYISAKSAYDEGNYDQALTNLSAAKSGNTKMEAAFQALRAKITKATSSSESSTNSASSVSETSSTNSASSSVANAAAANSTSESVISQFASKMGFGQQGYGIIPVSQNGDVYRFEVRQSNADNTVANLIGIYDYNADTGVVTQIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIQ7</accession>
    <name>H4GIQ7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zinc/iron ABC transporter substrate binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_14638</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the bacterial solute-binding protein 9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14638"/>
    <dbReference type="EnsemblBacteria" id="EHS87252">
        <property type="protein sequence ID" value="EHS87252"/>
        <property type="gene ID" value="PS3_14638"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.477"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006128">
        <property type="entry name" value="Lipoprotein_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006127">
        <property type="entry name" value="ZnuA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01297">
        <property type="entry name" value="ZnuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00690">
        <property type="entry name" value="ADHESNFAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1 3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="305" mass="34142" checksum="7015B390E5145C71" modified="2012-04-18" version="1">MIKKYWKIGLLVVVLLGVFGLMVTNWTPNKTSQKPIRVVTSLNFYGEVAKAVAGDHGQVTSFIDNEAVDPHDFQPSTKEAQKVAKANVVIVNGLGYDSWLTKLTKAADSSQKVINVGTQVADKKNGDNEHVWYQPATMNKLAIQLAKQFSKLDPEHKADYYKNAKAYQAKLTKLDAVIQTAKQNVGDQKLVDVSEPVFDYALNNLGYEVNNKHFEKAVEDDNDPSPQDIQQIRDDITQHKIAFFVNNSQESDKTVKNLVKLAQENNVPVLNVTETEPNGKSYVEWMTDQYKQLIKIQGSESSSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIR1</accession>
    <name>H4GIR1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_5403</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121945.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5403"/>
    <dbReference type="EnsemblBacteria" id="EHS87256">
        <property type="protein sequence ID" value="EHS87256"/>
        <property type="gene ID" value="PS3_5403"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.481"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004536">
        <property type="term" value="F:deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015991">
        <property type="entry name" value="Hydrolase_TatD-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00010">
        <property type="entry name" value="TIGR00010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01137">
        <property type="entry name" value="TATD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01091">
        <property type="entry name" value="TATD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="257" mass="29269" checksum="8FCEB99C37506328" modified="2012-04-18" version="1">MEIYDSHTHLNDEPFFDDVPAFLARAQHYQVTEMNIVGSNQLLNQRALELAHRYHQLHAVIGWHPEEFSDYQAATLDQLANQLADPAVVAVGEIGLDYYWDQDHHELQKQMLLDQISLAQQFHLPIVIHCREALSELYDILKMIDISSIGGVMHSFSGDVDWAKKFLDLGMYLSFSGVVTFNSAHEVQTSAQYVPADRLLVETDAPYLTPMPYRGKQNEPAFTAFVVEKIAQLRQVEPGELAKITTANARQLFKKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIS2</accession>
    <name>H4GIS2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uracil-DNA glycosylase superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121956.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13020"/>
    <dbReference type="EnsemblBacteria" id="EHS87267">
        <property type="protein sequence ID" value="EHS87267"/>
        <property type="gene ID" value="PS3_13020"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.494"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="SSF52141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDG" evidence="1">
        <location>
            <begin position="25"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="210" mass="23912" checksum="B3AE472C97BFB626" modified="2012-04-18" version="1">MHYPSELVEAIKAQTVDLKLEDFLEGAGPLHPHLMIVGEAPGREEIESHIPFHGASGKELMRFLNLAGYKREDVYITSVVRSRPYSVKQVKDRRTGKIVTKYPNRTPSNKEVRIYAPLFDWELHQVQPDVIIPVGNTALHRLLGPDEVISAVHGQLIQHPVLEFNSATGQYQWSQASYQIVPMYHPAAVLYRRQLMPTVEADWQQLSQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIS7</accession>
    <name>H4GIS7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Prenyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_13761</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Quinol/quinone metabolism; menaquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13761"/>
    <dbReference type="EnsemblBacteria" id="EHS87157">
        <property type="protein sequence ID" value="EHS87157"/>
        <property type="gene ID" value="PS3_13761"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.504"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd13962">
        <property type="entry name" value="PT_UbiA_UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026046">
        <property type="entry name" value="UBIAD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929">
        <property type="entry name" value="PTHR13929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005355">
        <property type="entry name" value="UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="292" mass="32530" checksum="3F4569D4FC7C4651" modified="2012-04-18" version="1">MLQSKTVVVSALPFIIGFLYAGVTYHQINWWGSFWLLVACVAFHFAVNAHNQLSDYYRFHKDQQIEGSHNNVIQRFQIPLWVAKLTIISLVIFTAIIGLTLVYQTGVLLLIIGIISFLVGYFYSGGPYPILKTPLGEPVSGITMGFNIVILGTYINVATTVHVDGAFLGRMLLVALPTILVISNLMLANNISDLVEDQRMGRHTLITFIGQVNGCRLWRICYYLAYLAIAVGIGSHVLPFSTIVVFLTFPIVEKKINEFIHDPDKATTFLNSIQIILLILVSEIIGLMLSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIT4</accession>
    <name>H4GIT4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">50S ribosomal protein L17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rplQ</name>
        <name evidence="6" type="ORF">PS3_10547</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit. Contacts protein L32.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the bacterial ribosomal protein bL17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121968.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10547"/>
    <dbReference type="EnsemblBacteria" id="EHS87164">
        <property type="protein sequence ID" value="EHS87164"/>
        <property type="gene ID" value="PS3_10547"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.513"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01368">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_L17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036373">
        <property type="entry name" value="Ribosomal_L17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="PTHR14413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64263">
        <property type="entry name" value="SSF64263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00059">
        <property type="entry name" value="L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01167">
        <property type="entry name" value="RIBOSOMAL_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="126" mass="14037" checksum="BC94D6B83D6F1E02" modified="2012-04-18" version="1">MSYRKLGRTSSQRKALLRDLTTSLIVNGSITTTEPRAKEVRKTMDQMITLAKRGDLASHRKAAAFVRNVVADVKEDGDDIKVQSALQSLFEDLAPKYADRNGGYTRILKTMPRRGDGAKMVILELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIT6</accession>
    <name>H4GIT6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">30S ribosomal protein S11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpsK</name>
        <name evidence="9" type="ORF">PS3_2991</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 30S ribosomal subunit. Interacts with proteins S7 and S18. Binds to IF-3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121970.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2991"/>
    <dbReference type="EnsemblBacteria" id="EHS87166">
        <property type="protein sequence ID" value="EHS87166"/>
        <property type="gene ID" value="PS3_2991"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.515"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019981">
        <property type="entry name" value="Ribosomal_S11_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_S11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_S11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="PTHR11759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03632">
        <property type="entry name" value="uS11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="129" mass="13745" checksum="56B323E2E8CC1EBF" modified="2012-04-18" version="1">MATKKGTRKRRVKKNVETGVAHIHSTFNNTLIMITDVQGNAVAWSSAGVLGFKGSRKSTPFAAQMASEAAAKQAMEHGMKTVEAEVKGPGSGRESAIRALQAAGLEVTAIRDVTPVPHNGSRPPKRRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIU0</accession>
    <name>H4GIU0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein translocase subunit SecY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secY</name>
        <name evidence="11" type="ORF">PS3_10172</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121973.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10172"/>
    <dbReference type="EnsemblBacteria" id="EHS87170">
        <property type="protein sequence ID" value="EHS87170"/>
        <property type="gene ID" value="PS3_10172"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.520"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01465">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026593">
        <property type="entry name" value="SecY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="PTHR10906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="SSF103491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="364"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="434" mass="47076" checksum="EAC797B5D52D17BD" modified="2012-04-18" version="1">MFSTVKDAFKVKEIRGKIFFTLFVLFVYRLGSYITVPGVNAGALQSLANSGLASILNTFSGGGLSNYSLFAMGVSPYITAQIVVQLLQMDIVPKFVEWSKQGEVGRRKLNQATRWLTIVLGFIQSIGITAGFSALSSIKLVETPGVTSYITIGLILTAGTMFATWLGDMITERGLGNGVSMIIFAGILAQIPGGIVQLWNDQIAGESGSALWEGIGFVAVVVIALLIIVQFVTWVQQAERRLRIQYTRRTTTAPANSYLPLKINVSGVIPVIFAGAFISTPQTILVAFQQAHSGDTWYQILTSIFNLSSGPGITLYTVLIVAFTFFYAFVQVNPEKLAENLQKQGSYITGVRPGRGTQDFVSKLLMRLSTVGSLFLGLISLIPLLASVWFGLSESIGLGGTSLLIVVQIALDVVRQLKGMTTKREYVGFIHEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIV1</accession>
    <name>H4GIV1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5 7">50S ribosomal protein L16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rplP</name>
        <name evidence="10" type="ORF">PS3_13021</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 7">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121984.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13021"/>
    <dbReference type="EnsemblBacteria" id="EHS87181">
        <property type="protein sequence ID" value="EHS87181"/>
        <property type="gene ID" value="PS3_13021"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.533"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01342">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_L10e/L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_L10e/L16_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000114">
        <property type="entry name" value="Ribosomal_L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020798">
        <property type="entry name" value="Ribosomal_L16_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220">
        <property type="entry name" value="PTHR12220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00060">
        <property type="entry name" value="RIBOSOMALL16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="SSF54686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01164">
        <property type="entry name" value="rplP_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00586">
        <property type="entry name" value="RIBOSOMAL_L16_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00701">
        <property type="entry name" value="RIBOSOMAL_L16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L16" evidence="9">
        <location>
            <begin position="4"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="143" mass="15900" checksum="41C7514A8D870C1B" modified="2012-04-18" version="1">MLVPKRVKHRKVQRGHMRGEAKGGKTVNFGEFGLQALDSHWISNRQIEAARIAMTRHMKRGGKVWIKIFPHHSYTSKGVGVRMGNGKGAPEGWVAPVKRGKVMFEVAGVPEEVAREALRLAGNKLPVRTKILQREVGGESNED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIV9</accession>
    <name>H4GIV9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GIY-YIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_21916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121992.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21916"/>
    <dbReference type="EnsemblBacteria" id="EHS87131">
        <property type="protein sequence ID" value="EHS87131"/>
        <property type="gene ID" value="PS3_21916"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.543"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018647">
        <property type="entry name" value="SLFN_3-like_DNA/RNA_helicase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09848">
        <property type="entry name" value="DUF2075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="36"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="569" mass="66231" checksum="C533344FB56BF452" modified="2012-04-18" version="1">MVSEPYIKEFSYSQDGVNLIKSLQISDNEQNRYIRDYPTVYVINDEKKDKYDVYVGETNNIVQRTLQHLNADPKNRDDWDDLKKAKNPNMYVIAHDHFNKSLTLDVENRLMLYLGGSQCVDRIKNRRTNEQGEYYPVEEFDEIFEKIWRELNKRNHSLFPAMEEIRNEAIFKASPFHKLTAEQLQAKFTILNKIREILSDPNSEDEHLIMVTGDAGAGKTVLLSSLFYDLTQDDENRDVHLMVNHNEHETVYRTISKKLGISTSKVTKPTNFINNYSKYNRADVVLVDEAHLLWTQGRQGYRGSNQLRDLLERAKVVIAIFDENQILRNQQILTNDQIKNLKEKALNTGDLIELHNQLRMQANDSTINWIKNLIENQTIGNLPEDDSYKVKVFDTPQQLEDAIDYENDLDVDSDYKNGISRLVATYDWPYSSTGKDVYKVKIGNWELPWNYQIKDHPKIVNYKDLSWVEKPNTIKEVGSIYSVQGFDLNYVGVILGPSIKYRDGKIIFDKSVHFNKDVTNNRTMENGEKKNFGEELIKNEMNVLMTRGVHGLFIYAVDEALREKLKSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIW2</accession>
    <name>H4GIW2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-oxoacyl-[acyl-carrier protein] reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_17224</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17224"/>
    <dbReference type="EnsemblBacteria" id="EHS87134">
        <property type="protein sequence ID" value="EHS87134"/>
        <property type="gene ID" value="PS3_17224"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.546"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="254" mass="26830" checksum="BD011359FC52EC0F" modified="2012-04-18" version="1">MKMTRPTAIITGGSRGIGAAMADKLGELGYNVVLDYHSDSSKGKAEAVAKSLEGKYGVETLVVQSDVSQYDDCVHLVQATVDKFGDQIDCLVNNAGITNNSNWIDIKPEAYERVIAINLMSALHMTHLVLPYMVNHSDKDHQCNIVSTTSVGGLTGVINQADYCASKSGLIGLTRGLALEYAGKGIRVNAIAPGMIMTDMLRGVNQDELKALSATIPQGYIGDVSDIAGALGYLLTASYVTGQVLSPNGGFVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIX4</accession>
    <name>H4GIX4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prolyl aminopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_8393</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8393"/>
    <dbReference type="EnsemblBacteria" id="EHS87146">
        <property type="protein sequence ID" value="EHS87146"/>
        <property type="gene ID" value="PS3_8393"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.566"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="173" mass="20412" checksum="5A5E1E43BA975283" modified="2012-04-18" version="1">MSQVEIRPVKLTEIKQLQHLMRVAYVQTYVEDHNHLDVNTYLNQDYSLDQIRQEMANPQSQYYFLVVHDRLAGYLKLNIGQAQTEPDYPDALEIQRIYILEAFQGLHLGSQLMEFAHERARFHGAKQIWLGVWEKNYDAMEFYAKFGFHQVGSHDFIQGSDVQTDYILLKELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIX9</accession>
    <name>H4GIX9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="2 6">6.3.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_8988</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 6">
            <text>ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-cysteine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glutamate--cysteine ligase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="2 6"/>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122012.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8988"/>
    <dbReference type="EnsemblBacteria" id="EHS87151">
        <property type="protein sequence ID" value="EHS87151"/>
        <property type="gene ID" value="PS3_8988"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.574"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007370">
        <property type="entry name" value="Glu_cys_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006334">
        <property type="entry name" value="Glut_cys_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38761">
        <property type="entry name" value="PTHR38761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04262">
        <property type="entry name" value="Glu_cys_ligase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glu_cys_ligase" evidence="7">
        <location>
            <begin position="12"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Glu_cys_ligase" evidence="7">
        <location>
            <begin position="261"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="506" mass="58756" checksum="FFCAA5C070605644" modified="2012-04-18" version="1">MVTVKQILNENPQLMDLLLEGNFGIELEQHRILTDGQLSRYPYPQVFRSRRHNPYLKTDFSDTMTEIASAPTLGATAAVKNTKILQQIVHDQLHEDERYWPLSVLPELNDADLEYAGHNNTRHWMDDYHDYLQEKYGTVRQIMTGVHVGYSLNDSLIYGLYHHGFKEKYASMTEFRNEIYFQLAQGFVLNRWLFTYLFGCSPLLTNRPTDLPAEIQGPMRSFRSSKYGYANLPDEEVAYGSFDQFINQIQQYVDDGTYFSHHEFYGPVRLKTPADTLADIKNNGTRWIEIRAFDLDPLSRSGISNDTLNFLELYLTYTLTLEEPAELNARLVEAVELNNQVALQNPTEQFDWVKDRASELLDQIEAFADEVNAPKIYQDALLFTRRRVEDPSLTISGQIMEKLISPEDYLNYGLLIANDRFSMNRHYEHPIEVLKDQYPENTQQLLRAAIEMGVTVIFEDDSHFELSVGDHLEFFDKTIKFIFPEGPEQYLANMFPELAELTDEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ14</accession>
    <name>H4GJ14_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Beta-hydroxyacyl-ACP dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_4381</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 4-hydroxybenzoyl-CoA thioesterase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thioester dehydratase family. FabZ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122047.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4381"/>
    <dbReference type="EnsemblBacteria" id="EHS87126">
        <property type="protein sequence ID" value="EHS87126"/>
        <property type="gene ID" value="PS3_4381"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.632"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006684">
        <property type="entry name" value="YbgC/YbaW"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003230">
        <property type="entry name" value="YbgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00051">
        <property type="entry name" value="TIGR00051"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4HBT" evidence="6">
        <location>
            <begin position="18"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="138" mass="15702" checksum="60A667ED4E264B09" modified="2012-04-18" version="1">MKSYIHRVHYYETDKMAITHHSNYIRWMEEARVDFLDQIGWNFAKLEALGIASPVVKVDCTYKVPTKFDDLVEITTSIDKYTGVKLVLHYVMKLAGTDTIVCVAESAHAFTNEAGRLISIKKSLPEMDQSLKQCLASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ70</accession>
    <name>H4GJ70_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-octaprenylphenol hydroxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_14607</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122103.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14607"/>
    <dbReference type="EnsemblBacteria" id="EHS87025">
        <property type="protein sequence ID" value="EHS87025"/>
        <property type="gene ID" value="PS3_14607"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.717"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="514"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="545"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="ABC1" evidence="2">
        <location>
            <begin position="70"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="569" mass="64215" checksum="B9EE71682D20B0EE" modified="2012-04-18" version="1">MTTISGKKRLLQIISVLKKYSFISNFYRQTNPDQIRQALEELGPTFIKLGQILSTRPDLVSPALINELQQLQDKVKADDYEVIAQIYEEQTGQTIAENFASFEETAFASASIGQCHHATLLDGTPVVVKIQHPDVSTLVQVDLNLFRRAVKMLKYVPNDGKSVVDLPQVFDQLSTSLMNEIDTLHEVKNGERFYDLNNGQGVFVVPQVYPNESSTKVLVDQAMTGDSIKQLFASEEHPLSTDEQARNTAIGHQLVENFIKQVFTDHFFHADPHPGNILYRPVTSSDQPNVSIDTKTKTFNHLEFEYQKASELPPFRLIYLDFGMMGTLSNELADGIANIIIALVQKDIYKVSQAVLAVCNRTGQVDEQQFNQQFGIFIRPYLNQGLGDIDFGTMVFEITRLCQTNHLQMKPEVTMLMKAFATLEGTVAKLDPTISMMAVARPFAKKYLKEHFNWRESLGDASLAMYSSLESLSKLPNQVNRLMTILASGEQQFNFHYQGQDKVLKSLHKMVNQLATVIVIAAVIMGSSIMVVGSSDHPFIHDFGIWAYVIAIFIVVIMMISWLWKSWRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ85</accession>
    <name>H4GJ85_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Iron compound ABC transporter ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_10210</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122118.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10210"/>
    <dbReference type="EnsemblBacteria" id="EHS87040">
        <property type="protein sequence ID" value="EHS87040"/>
        <property type="gene ID" value="PS3_10210"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.744"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="261" mass="29348" checksum="38EF24B5ED6A7240" modified="2012-04-18" version="1">MISVKEVSFQYDHQPRLIDNLNFSLPSKKITAIVGPNGSGKTTLLKLLMRSLKPEEGEVTIAGQSIWNLSAKEFAQQVAVVHQHNPLYDVIKVGELIDFGQLPYHDLLTSADRSDQGKDAIIQQLGLTELVDRPMGSLSGGQQQLVWLATALNQSPQILVLDEPTTYLDLYYQVKLMHLIQKIQKTQQLTIIMILHDLNQVLHYADWCLLLKDGQLIDQGLPKEILTPKQIQATFNLATDMINMPQGVQLYIKNEQPLMGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJA2</accession>
    <name>H4GJA2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">SPP1 Gp6-like phage portal protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_11374</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000031">
        <property type="protein sequence ID" value="EHS87014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11374"/>
    <dbReference type="EnsemblBacteria" id="EHS87014">
        <property type="protein sequence ID" value="EHS87014"/>
        <property type="gene ID" value="PS3_11374"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.786"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021145">
        <property type="entry name" value="Portal_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006428">
        <property type="entry name" value="Portal_SPP1-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05133">
        <property type="entry name" value="Phage_prot_Gp6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01538">
        <property type="entry name" value="portal_SPP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="458" mass="51878" checksum="D6DDD91300018ED5" modified="2012-04-18" version="1">MTKQGVYLFNGDQIDVPDLRPFYIEQDNRVNHYESNYDIYAGQHAILRKAVDPDSWRPDNRLVSNWGAYVVDTYSGYFMGRKPTISLDETTTNDRLQDWLNESSLLDILTECSKQISIYGRSYLLGFQDEESRTNTISVEPTNGFLVYSTSVKNKPIGFVRYNVYNNKRIGELYTDSEVIEFEDDRITDRHNHLFGEVPAVELYANVERLSLIDKVKSLVETYDEALSQKVNQNEYFDNALLKILGIPFEHDETGQPIIDLKNRVLVAEDPDSKDGVIDFVSKPDNDGTQENLLNRIKDDIFQTAMVANLNDKVFSGNTSGVAIKYKLLPMQNQALNEERKFTIALRKFLSTLLSLGQIIGTADKDSLMRDLTFKFYRNVPEDLANEADTAQKLDGIVSQETQLSTLSFVDDPKTEMQRMKDEGIDKALTAVKTSASATDLITNQSGGLNGQEDKPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJB1</accession>
    <name>H4GJB1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_3986</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000032">
        <property type="protein sequence ID" value="EHS87001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3986"/>
    <dbReference type="EnsemblBacteria" id="EHS87001">
        <property type="protein sequence ID" value="EHS87001"/>
        <property type="gene ID" value="PS3_3986"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.804"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="192" mass="20263" checksum="0DC27FC10F29FE89" modified="2012-04-18" version="1">MLMPSVISIYIIITGNKLLANQVDRGSMAYILSAPIRRTTVVTTQAIYFIGSLAATFLTTTGTFMMVNNMADTGFANDVIVYLNLGAFMVSLALAGIMFAASGIFNLSKNVMGTGGLLVLAFLIIAIVGSFADFGVPDLKPMQHFTILSLFDIKNILAGGSDWIPKMAVLGGIGLGTITLGGLSFVKKDLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJE5</accession>
    <name>H4GJE5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_2749</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000036">
        <property type="protein sequence ID" value="EHS86868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122178.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000036.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2749"/>
    <dbReference type="EnsemblBacteria" id="EHS86868">
        <property type="protein sequence ID" value="EHS86868"/>
        <property type="gene ID" value="PS3_2749"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.857"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="2"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="7">
        <location>
            <begin position="134"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="134"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="239" mass="27516" checksum="E899FC1A23C36F4E" modified="2012-04-18" version="1">MKILMIEDNNSVSAMMAMFFKKEQWEATFAYDGEEGVDKFNEANGDYDIILLDLNLPKKDGMQVAADIRRESPTIPIIMLTARDSESDQVLGLEMGADDYVTKPFSPITLIARIKALHRRIQLSQISDDTETENSEYDVQTDHFKLNSKTREAYINDQLIEDLTPKEFDLLKTMAMKPKQVFTREQLLQLVWNYEFYGDERTVDAHIKKLRQKIEKAGPKVIQTVWGVGYKFDDSDAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJG5</accession>
    <name>H4GJG5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13393</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000039">
        <property type="protein sequence ID" value="EHS86608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122198.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000039.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS86608">
        <property type="protein sequence ID" value="EHS86608"/>
        <property type="gene ID" value="PS3_13393"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.916"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009825">
        <property type="entry name" value="ECF_substrate-spec-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07155">
        <property type="entry name" value="ECF-ribofla_trS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="161" mass="17360" checksum="91880754FD5B1CF2" modified="2012-04-18" version="1">MRTTNIRKITISAMLMALLIIFGRVFLIPIPWTHGNVNLCDAAVFLASLLFGKWYGGTVGGFGALLLDLISGYANFAPFSFLAHGLEGFLAGWLYTRTHNRFLALGVGIVVMVAVYVVANGVLYTWVAGILGIGTDLLQGIIGAVVAALVYQPLKRWGNNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJI6</accession>
    <name>H4GJI6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FeS assembly protein SufB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_13758</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0051 (ycf24) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000041">
        <property type="protein sequence ID" value="EHS86576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122219.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000041.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13758"/>
    <dbReference type="EnsemblBacteria" id="EHS86576">
        <property type="protein sequence ID" value="EHS86576"/>
        <property type="gene ID" value="PS3_13758"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.945"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010231">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045595">
        <property type="entry name" value="SufBD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19295">
        <property type="entry name" value="SufBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="SSF101960"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01980">
        <property type="entry name" value="sufB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SufBD_N" evidence="2">
        <location>
            <begin position="131"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="467" mass="52726" checksum="1191458A90F7AD54" modified="2012-04-18" version="1">MAGAADVLNDQEYEFGFHDNIEPIYSTGRGLTEETVRQISAEKDEPEWMLEYRLKCYHEYINMPLPQFGPDLSQLNLDEMLYYQKMTDKKFRDWDEVPEDLKRTFDRLGVPEAERKYLAGSSAQYESEVVYHNMKDEFEKQGIIFTDTDSALKEYPELFKKWFGKLVQPTDNKFAALNGAVWSGGSFIYVPKGVKVMTPIQSYFRLNYENTGQFERTLIIVDEGASVDYVEGCTAPNYSSDSLHAAVVEVNVLPDAYCRYTTIQNWSDNVYSLETKRAAADKNATMEWVDGNLGSKVTMKYPSVYLNGEGARGTMLSIAVASNGIHQDSGARMIHNAPKTSSSIISKSISRTGGSTDYRGTVRFAKNSSGSKAHVECDTIIMDDQSSSNTIPYNEIDNPNVAMEHEAKVSKISEKQLYYLMSRGLSEKKATEMIIMGFVEPFTKELPMEYAVELNRLISFEMEGSIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJJ1</accession>
    <name>H4GJJ1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4767 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_3333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3333"/>
    <dbReference type="EnsemblBacteria" id="EHS86501">
        <property type="protein sequence ID" value="EHS86501"/>
        <property type="gene ID" value="PS3_3333"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.952"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031927">
        <property type="entry name" value="DUF4767"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15983">
        <property type="entry name" value="DUF4767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4767" evidence="1">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF15983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="142" mass="15538" checksum="4E6F19D7501FA742" modified="2012-04-18" version="1">MWNTSKDQALANFMASWAPKMGQSYVKYDGTNSIKSASGMTYPDDFDIAYFQSTPAKGAGNGTETKISMGWAPNGQGPYDYNVVAIYNYNSGKAEGRITYAFCVHNGQPVALVNQTTNGNDVWTVTQNQDVSSNFAKIFNEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJJ2</accession>
    <name>H4GJJ2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Multidrug transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_12639</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the major facilitator superfamily. EmrB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122225.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS86502">
        <property type="protein sequence ID" value="EHS86502"/>
        <property type="gene ID" value="PS3_12639"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.954"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="272"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="362"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="450"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="9">
        <location>
            <begin position="19"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS86502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="495" mass="53442" checksum="4D57E9F0E46AD45B" modified="2012-04-18" version="1">MNSNQPTDIYGKPYHRGLFVAVFLIGAFVMVLNQTVISTAQPAIMKSFDIGTSTVQWLTTGFSLVNGIMIPISAWLMTRFRTKPLILVALASFFGGTLVAFCANSFGMLLAGRLIQALGAGAIMPLMQTVFLTIYPPEQRGAAMGINGIVIGLAPAVGPTLSGWVVDSMTWRDLFGIILPIVAVAFIGSIFFIKDVLPIKKTKLDLLSVITSTIGFGTLLYGFSNAGTDGWTDTWVLLEILIGVVFVIIFAWRQLKMDEPFLNLRVFKSVEFTMAALISAVATLAMTGAEIILPLYIQNLRGISAFHSGLLLLPGALMIAVIAPIAGRIFDRYGAKDMTILGMFLLSIGTVPFFFLTKDTSMAYIVIFYAFRMAGIAMALMTVTTAGMNALPMELMSHGTASNNTFRQVMSSIGVAVMTSVLTNVTKNAMPSSSVLHNTPLKYRDEAINAALSGYHATFILATGFAVVALILSLFLKSKPNRSTDIDVDKLKEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJJ7</accession>
    <name>H4GJJ7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">6-phospho-beta-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_22333</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122230.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_22333"/>
    <dbReference type="EnsemblBacteria" id="EHS86507">
        <property type="protein sequence ID" value="EHS86507"/>
        <property type="gene ID" value="PS3_22333"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.966"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018120">
        <property type="entry name" value="Glyco_hydro_1_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="Glyco_hydro_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="PTHR10353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00572">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS86507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="479" mass="54952" checksum="4C02D20EE1F5A2BE" modified="2012-04-18" version="1">MYQTAMPKGFLWGGAVAAHQLEGAWNVDGKGPSVADVMTAGANGVPREITDGVIAGKNYPNHEAIDFYHHYKEDIKLMAEMGFKCFRTSIAWTRIFPQGDEEEPNEAGLKFYDDLFDECHKYGIEPVITLHHFEMPWHLVEQYGGWQSRQVIDFFTRYARVVFERYQDKVKYWMTFNEIDNQSAFNNAFLMATNSGIIFKDGQDREAAMYQAAHYELVASALAVKIGHEINPDLQIGCMINFQTVYPATCSPDDALLAQRVGQRRYWFSDVHVHGEYPAEVEAYLNQKGYRPDITEEDRQVLREGTVDYVGFSYYNSMTVSAKKVDADHLGDLNESTVDNPYLDKTDWDWEVDPEGLRYALNWLTDRYHKPLFIVENGMGAYDQVTEDGRIHDDYRIDYLWRHISEVEKAVALDGVDLMGYTPWGCIDLVSAGTGQMSKRYGFIYVDKDDEGNGSLQRSRKDSFYWYQQVIASNGAKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJM7</accession>
    <name>H4GJM7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Isopentenyl-diphosphate delta-isomerase</fullName>
            <shortName evidence="6">IPP isomerase</shortName>
            <ecNumber evidence="6">5.3.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Isopentenyl diphosphate:dimethylallyl diphosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Isopentenyl pyrophosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Type 2 isopentenyl diphosphate isomerase</fullName>
            <shortName evidence="6">IDI-2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fni</name>
        <name evidence="8" type="ORF">PS3_19778</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biosynthesis of isoprenoids. Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its allylic isomer, dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5 6">Homooctamer. Dimer of tetramers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the IPP isomerase type 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000043">
        <property type="protein sequence ID" value="EHS86442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122260.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000043.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19778"/>
    <dbReference type="EnsemblBacteria" id="EHS86442">
        <property type="protein sequence ID" value="EHS86442"/>
        <property type="gene ID" value="PS3_19778"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1006"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02811">
        <property type="entry name" value="IDI-2_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00354">
        <property type="entry name" value="Idi_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011179">
        <property type="entry name" value="IPdP_isomerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43665">
        <property type="entry name" value="PTHR43665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003314">
        <property type="entry name" value="IPP_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02151">
        <property type="entry name" value="IPP_isom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0288">FMN</keyword>
    <keyword evidence="6 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 6" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0521">NADP</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_dh" evidence="7">
        <location>
            <begin position="157"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="8"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="67"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="286"/>
            <end position="287"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="351" mass="38745" checksum="3D96192B09E973B7" modified="2012-04-18" version="1">MVNQHAQRKNEHLSLAEKDYDFNHQYDPFAGIQLIPRALPETDLNKIQTHANLTDKLDLAWPFYLEAMTGGSERAGKINHQLATVAKEYDLAMATGSMSIIFKDDQAKASFTIVRETNPNGIIIANLGASATPQQAQAAIDLIQANALEIHLNVAQELIMPEGDREFLWLENLQAIQAAIKVPLIVKEVGSGMSVNDLNQLANAGIQVVNISGRGGTSFPRIENRRNHHRNYHDNLLAWGLTTPQALIESQFRHNANQQIIASGGITSPLDVIKAGAMGAQAVGVAGYFLHQLIQDGPETLSQEIAEWQAEIPEIMTMLGVSSFDQLKQVDLVLDPELYNYQKQRQTQIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJM9</accession>
    <name>H4GJM9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Branched-chain amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_9005</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AzlC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000044">
        <property type="protein sequence ID" value="EHS86252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9005"/>
    <dbReference type="EnsemblBacteria" id="EHS86252">
        <property type="protein sequence ID" value="EHS86252"/>
        <property type="gene ID" value="PS3_9005"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1010"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011606">
        <property type="entry name" value="Brnchd-chn_aa_trnsp_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34979">
        <property type="entry name" value="PTHR34979"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03591">
        <property type="entry name" value="AzlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="152"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS86252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="273" mass="30639" checksum="C8D88B52E209C6EB" modified="2012-04-18" version="1">MTQHHVNPEKRWLSVLGDTMPLCLSYAPIGLACGILLHAAGFNFIMAFLVSILVYSGGAQFILASMLVTNAPLSTIFMTIFFLEMRYALLGSSLSKYLHEDKPWDVFLFAFSLNDENYAINYLKYATDKNWTIKDATMVEHFALISWTGGNLIGALVGSTISIDLDIIEFALTALFIYMLVMQVRNHLTLLICLLAGALSVLFLVLIQNTLGLIISTLMASLIGFAVEDYVRKHSKHPKRTWILGEIFRPSTIRQTVEDKRKKQDQSQEAANH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJN4</accession>
    <name>H4GJN4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Inosine-5'-monophosphate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_10168</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000045">
        <property type="protein sequence ID" value="EHS86235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122267.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000045.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10168"/>
    <dbReference type="EnsemblBacteria" id="EHS86235">
        <property type="protein sequence ID" value="EHS86235"/>
        <property type="gene ID" value="PS3_10168"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="PTHR11911"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMPDH" evidence="5">
        <location>
            <begin position="12"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS86235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="367" mass="38380" checksum="B2EF9E0A165D2243" modified="2012-04-18" version="1" fragment="single">MSNWETKFAKQGLTFDDVLLIPAESNVLPNEVDLSVQLTDNIKLNLPFISAGMDTVTESSMAIAMALQGGMGVIHKNMSIQGQASEVANVKGVSLNQNFNRAAVDSQNRLLVAAAVGVTSDTFDRAEALFNAGADAIVIDTAHGHSAGVLRKIKEIREHFPDNTLIAGNVATAEGTRALFEAGVDVVKVGIGPGSICTTRVVAGVGVPQITAIYDAASVAREYGKSIIADGGIKYSGDIVKALAAGGNAVMLGSMLSGTTEAPGDLFEDNGHKFKSYRGMGSIGAMAQSHGSSDRYFQGGVNEANKLVPEGIEARVEYKGDVADVVFQMVGGLRSGMGYCGAENIQKLIDTAQFVKITNAGLRESHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJP8</accession>
    <name>H4GJP8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fumarate reductase flavoprotein subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_3521</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000045">
        <property type="protein sequence ID" value="EHS86249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122281.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000045.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3521"/>
    <dbReference type="EnsemblBacteria" id="EHS86249">
        <property type="protein sequence ID" value="EHS86249"/>
        <property type="gene ID" value="PS3_3521"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1038"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="3">
        <location>
            <begin position="10"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="483" mass="52207" checksum="CE6C72E3532A3B55" modified="2012-04-18" version="1">MSLPKEDQYDVVVVGSGAAGFSAGLTAVQGGSKTLVLEKGRTTGGSSNYTEGLFAVNSSLQKAKGIKVSGTEVLKEEVEYSQYKADARIWRNYVDGSAENVDWLQEQGVEFERVQAMGAGEATWHIYKGMGDAVIHQALQPRFETAGGEVVTSAAAVNLVRQADQTFVVTVRDEANQDVKAVHARAVILATGGYLNNPEMMAEETNYDLSRLETVSSGKGTGDGLRMAWQVGARKYGTGMAMLFGGYLSDPETPSFKFMSSQMNVAAGQQPLLWVNENGERFVNEEVVYNFSQAGNALYTQAEVYSILDQGVIDKMITEGNFMGLGVYVLRGQKMDTLQEEIDAAVAADKAFIFKADSIEELSQKMGLAQLPATISRYNRDAAQGEDREFGKPVEYMQPVTQGPFYGFKLNIGAFCTMGGLQVTPQNEVRDEQGQVIPGLYAVGNDAAGLTGDTYGPNMPGTCVGYAIYSGRNAGQQVTEYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJS4</accession>
    <name>H4GJS4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">O-acetylhomoserine (Thiol)-lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_4931</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122307.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4931"/>
    <dbReference type="EnsemblBacteria" id="EHS86111">
        <property type="protein sequence ID" value="EHS86111"/>
        <property type="gene ID" value="PS3_4931"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1093"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006235">
        <property type="entry name" value="OAc-hSer/O-AcSer_sulfhydrylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01326">
        <property type="entry name" value="OAH_OAS_sulfhy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="432" mass="46011" checksum="910E8917278BC339" modified="2012-04-18" version="1">MSVYDLDTNGLSFETLQVHAGQHPDAETGSRAVPIYQTTAYVFKDAEQAAGRFALTDAGNIYSRLTNPTTEVFEKRVAALENGTGGVALGSGAAAVTAAIQNVAGQGDEIVSASTIYGGTYNLFAATLPQFGITTHFVDPDDPANFEKAINDHTKVVYLETIGNPNANLIDIPAVAEIAHKHGILLIVDNTFGTPYLIRPLDLGADIVVHSATKFIGGHGTSMGGVVVEGDKFDYEASGRYPGFTTPDQHYNGLVWKDLGGAAFTTKLRAQILRDTGAALSPFNSFLLLQGLESLSLRIERHVENAQKVADYLNNHPKVAWINYPSLAGNKYKPLADKFFPKGVGSIFTIGLTGGAEAAVKFIDNLKLFSLLANVGDAKSLVVHPASTTHAQLSEEELLSAGITPDLIRLSIGIENVDDLIADLDQSLAQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJT0</accession>
    <name>H4GJT0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Amino acid ABC transporter ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_7737</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122313.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7737"/>
    <dbReference type="EnsemblBacteria" id="EHS86117">
        <property type="protein sequence ID" value="EHS86117"/>
        <property type="gene ID" value="PS3_7737"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1106"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015424">
        <property type="term" value="F:ABC-type amino acid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030679">
        <property type="entry name" value="ABC_ATPase_HisP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039085">
        <property type="entry name" value="ABC_ATPase_HisP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="244" mass="27471" checksum="1DF20038EF2B7380" modified="2012-04-18" version="1">MISFQNVDKQFADHHALDNVSVEFPEHQTTVLVGPSGAGKSTLLRSINLLERPQAGLLKINDQTIDFAKPIARNELLKARQQTAMVFQSWNLFPHLTILQNITEGPIVVHKQPKKEAEANARRLLEQVGLADTADRYPDQLSGGQQQRISICRALALDPSYILLDEPTSALDPELEAQVLRVLQDLAKQDQSMIVVTHNMEFARRVADKIVFVEDGRIEFDGSTDDFFTHPTERIQAFLDAMEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJU1</accession>
    <name>H4GJU1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Acyl carrier protein</fullName>
            <shortName evidence="1">ACP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">acpP</name>
        <name evidence="5" type="ORF">PS3_6174</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 3">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by AcpS. This modification is essential for activity because fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group.</text>
    </comment>
    <comment type="PTM">
        <text evidence="3">4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by acpS.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122324.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6174"/>
    <dbReference type="EnsemblBacteria" id="EHS86128">
        <property type="protein sequence ID" value="EHS86128"/>
        <property type="gene ID" value="PS3_6174"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1119"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="Acyl_carrier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="PTHR20863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00517">
        <property type="entry name" value="acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1 2 3" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="4">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="1 2">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="80" mass="8990" checksum="06320939BDE826F6" modified="2012-04-18" version="1">MDRAEVFKKVSAIVADHFEVDRAKITDNLNLRTDLNADSIDFVEFVLEVEDTFGATIDDDDAEQLSTIGEVVDYIVSHQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJY0</accession>
    <name>H4GJY0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter ATP-binding membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_18011</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS85997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122363.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18011"/>
    <dbReference type="EnsemblBacteria" id="EHS85997">
        <property type="protein sequence ID" value="EHS85997"/>
        <property type="gene ID" value="PS3_18011"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1176"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="19"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="325"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="359"/>
            <end position="366"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="535" mass="58701" checksum="144D0E08333DFB5B" modified="2012-04-18" version="1">MSALFRLFKLIAPLTGIMLAAICLGVLGQLTVIALPVIAITGLLTDHGNFINYLWPILICGFLRGPLHYGEHYCNHLIAFKLLALLRQKVFSALRRLSPAILENKSKGDLIALVTSDIEMLELFYAHTISPVAIATLVSIMVAGVLTWMSPWLGLWALLGYLLIGYLIPLVSHHIMDARGLQVRNQLGQLSSYVLDSLYGLGELLQFKGMPKRLQSLTDQTKALNHTQRQISLGTGLQNAAIYLIVFGFSLGTLLIAYALHLSPVNIIIATVIVLSSFGPVIALSSLTAGLSQTIGAAQRLVNLLDEEPIVQDVEQGQQPSYDQAQLTNVTFTYPDTVNPVIQNLNLTIPKGQIMGIHAPSGTGKSTILKLLARFYDPDTGQITINKTSLIEINSNYLRQMESYVAQETWLFQDTIANNLKLGHPKASDQEIQLAAQQAGIANYIESLPQQYQTKLSELADSISEGQKQRLGIARAFLHEGELLLLDEITANLDVLNEAIILKSLQKIAKEKTVVIVSHRLSTLSIADQIYELAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJY2</accession>
    <name>H4GJY2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Glutamine ABC transporter, glutamine-binding protein/permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_20481</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS85999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20481"/>
    <dbReference type="EnsemblBacteria" id="EHS85999">
        <property type="protein sequence ID" value="EHS85999"/>
        <property type="gene ID" value="PS3_20481"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1178"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043429">
        <property type="entry name" value="ArtM/GltK/GlnP/TcyL/YhdX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614">
        <property type="entry name" value="PTHR30614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="449"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="282"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="481" mass="51677" checksum="1B3C60A38A1DD2C6" modified="2012-04-18" version="1">MAASSSSSSSEATSSSASSTSGDKLAEIKQKGVLVVGLSADYPPLEFTAKINGKTEYVGVDVSIAKAIAKDLGVKLEIKNMSFDSLLVAMETGKVDMVISGMNATAERKQSVDFSHVYYKGGATFLINKKDKAKYKSGNDLKTANIGVQTGTVQATMAKNSFPNATVKPLSNLSNLVLALKSGKTNAVLMDALIAKAYASNDDSLAVVSKTGIKNDLSDAGTAVAMPKGQTSLVNAVNQTIDRINQNKTVEKTYLPAAAKYMKKVEAKRTLWNYWTYFAKGIGDTLIITVVSVFFGFVLGILLSLMRLSKIKLLHWIAVCYIEFVRGTPLMVQIMFIYFGIGAVISSMPAILAGIIAISLNSGAYVAEIIRSGIQSINKGQSEAARSLGLSQKMTFQYVVMPQALKNIWPALGNEFITLLKDSSLASTIGVAELMYQTQLVQTSTYKGVAPLAITMVLYFIMTFTVSKILGYYEGKMNHDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJY4</accession>
    <name>H4GJY4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_11221</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS86001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122367.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11221"/>
    <dbReference type="EnsemblBacteria" id="EHS86001">
        <property type="protein sequence ID" value="EHS86001"/>
        <property type="gene ID" value="PS3_11221"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1185"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039369">
        <property type="entry name" value="LacA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43017">
        <property type="entry name" value="PTHR43017"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="185" mass="20400" checksum="9DEAA83E88657C0E" modified="2012-04-18" version="1">MGVFENLRKGQHYAISDPDYQAEVHQEINRSEHLSYLISQTDPNEQKRILDLENQLLNDQMHDGTYFTPPFNIDCGSRVFLGKNVFANHDLNIMSAGTITIEDGVMMGPEVALLTVNHEPKNIRTVYTGEIHIKKNAWLGARVSVLPGVTIGENAIVGTGAVVTHDVPDNTIVAGVPARVIKTID</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK00</accession>
    <name>H4GK00_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Glutamate decarboxylase</fullName>
            <ecNumber evidence="3 7">4.1.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_14606</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H(+) + L-glutamate = 4-aminobutanoate + CO2</text>
            <dbReference type="Rhea" id="RHEA:17785"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="4.1.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 5 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the group II decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.15" evidence="3 7"/>
    <dbReference type="EMBL" id="AICN01000052">
        <property type="protein sequence ID" value="EHS85882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14606"/>
    <dbReference type="EnsemblBacteria" id="EHS85882">
        <property type="protein sequence ID" value="EHS85882"/>
        <property type="gene ID" value="PS3_14606"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1217"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004351">
        <property type="term" value="F:glutamate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006536">
        <property type="term" value="P:glutamate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010107">
        <property type="entry name" value="Glutamate_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321">
        <property type="entry name" value="PTHR43321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01788">
        <property type="entry name" value="Glu-decarb-GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="435" mass="50270" checksum="ED3F32B7419A966D" modified="2012-04-18" version="1">MNQQPTEPRIAYQVIKDQLLDEGNARQNLATFCQTYMEPEATKLMSETLEKNAIDKSEYPRTAEIENRCVNIIANLWHVQSNEEYMGTSTVGSSEACMLGGMAMKFAWRNRAKKLGLDLNAHKPNLVISSGYQVCWEKFCVYWDVELRTVPMDLNHQSLNMEKVMDYVDEYTIGIVGIMGITYTGRYDDLEKLNDLVENYNQKTDYKVYIHVDAASGGLYAPFMEPDLKWDFQLKNVVSINTSGHKYGLVYPGIGWVLWRDQAYLPKELIFNVSYLGGEMPTMAINFSRSASHIIGQYYNFIRYGFKGYHDIHQRTHDVAVYLAKEIEKLDLFEIVNDGSRLPIVCYRNKDNREHEWTLYDLADRLAMKGWQVPAYPLPADLDQIEVQRIVVRADFGMGMAHDFIEDLKQAIHDLDQAHIVFHEKTALKKYGFTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK19</accession>
    <name>H4GK19_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Xaa-Pro dipeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_14269</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000054">
        <property type="protein sequence ID" value="EHS85871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122402.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000054.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14269"/>
    <dbReference type="MEROPS" id="M24.006"/>
    <dbReference type="EnsemblBacteria" id="EHS85871">
        <property type="protein sequence ID" value="EHS85871"/>
        <property type="gene ID" value="PS3_14269"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1247"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_NTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="SSF53092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase_N" evidence="2">
        <location>
            <begin position="6"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M24" evidence="1">
        <location>
            <begin position="147"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="367" mass="41110" checksum="12581EB34A455125" modified="2012-04-18" version="1">MAYEKLAELQTTLQNHQIDVAYLSDPMTIKYLTGFGSDPYERILALIVFPDQDPFLFGPALEVEAIKGTGWPYPVYGYLDHEQPFAMIAEQIKKRLPDPHQIATEQNYLTVSRQRALQEQFPHAKFDHDLTQTIEHMRLIKSADEIEKLKIAGQWADFAFETGFKAVQIGRTEQEISAEIEYALKKKGIMEMSFDTLIQVGPHAAEPHGETSTNQVQNNELVLFDLGTVFDGYVSDASRTIAVGTLSDRQKEIYQVCLEAQLTAQAAAKPGITAAELDKIARDVIDHAGYGEYFIHRLGHGMGMSDHEFPSIMEGNDLVLEPGMCFSIEPGIYIPGFAGVRIEDCVVITPDGNEPFTHMTKELQYFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK35</accession>
    <name>H4GK35_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cyclopropane-fatty-acyl-phospholipid synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_10206</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFA/CMAS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000055">
        <property type="protein sequence ID" value="EHS85851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122418.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000055.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10206"/>
    <dbReference type="EnsemblBacteria" id="EHS85851">
        <property type="protein sequence ID" value="EHS85851"/>
        <property type="gene ID" value="PS3_10206"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1267"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003333">
        <property type="entry name" value="Mycolic_cyclopropane_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003085">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="2">
        <location>
            <position position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR003085-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="394" mass="45364" checksum="C8CDA5F600FC43FE" modified="2012-04-18" version="1">MLEKTFYKQLLSRSFNGLTVKVIYWDGRSAIYGDGEPEVTIKFNEKISIREITKNASITLGEAIMDKKIEVDGSLQKLIFAAYNSADSFMRSSKYRRFLPKQRGHSEQQSKNDVQSHYDVGNDFYQIWLDPTMTYSCAYFTDNNVDNDLEQAQIDKVHHILKKLDPQPGKTLLDIGCGWGTLMLTAAREYGLKVTGVTLSEEQFKHVQRTIDEEHLADVAEVHLIDYRELGDQQWDYITSVGMFEHVGKENLGLYFQDVARYLKDDGVALIHGITRQQGGAYNGWIDKYIFPGGYIAGLTELIDHIEDAHLQIADMEMLRRHYQRTLEIWDRNFNEHRGEITDMMGERFVLMWDLYLQACAASFESGNIDVIQYLITKGASGKSLPMTRDYMHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK45</accession>
    <name>H4GK45_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative sensor kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_16939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000056">
        <property type="protein sequence ID" value="EHS85812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122428.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000056.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16939"/>
    <dbReference type="EnsemblBacteria" id="EHS85812">
        <property type="protein sequence ID" value="EHS85812"/>
        <property type="gene ID" value="PS3_16939"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1281"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="349"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="592" mass="67600" checksum="3A31ABD62BDE6A2C" modified="2012-04-18" version="1">MTKQINWYKWIFAGLVMVSLGIYFTIALRHSANIGISVRQQHGQWIITRILSHSAGDNISVAVGDQLLQIDGNDPGKVSSVQRMLLVNHASKLTILRDGHQQVISLASHPFLSTYWILLAVSLSVILITVLILFWRRQSLQERHFENFMLFFAIALVALIPSSQGIILGRIILVAFLSLIPMMVRKMLVSPKINSFLGWLTLIYTYLNPLLYIAFVLGWLPAWYWLADYLNDWGSFFLPLVLVILVFLEGIQKGQERVSRVNFSLIFLTSLIPLYLLYLYPLKWPPQHSFLNVAAFLLVPIICLLHILIVNRSLSNSYRRVRGYAQGYLLVLTTTIMALIINLGRGLPLWLVTAYALMLMIAVAPVYQEVFALIAGFDSRHQRLELFDAVENEREQVALTIHDTAIQNLILMKKQFERASPEDNHQKLLDTMDDLIHELRDLCNRVYPMLISDLGVANTIRQMIDHLQQTWPIEIQFQDHSGGQLDQLSDRQANFVLRSLQEMLINSVKHGQANQAWVQVTRNQKSLTITCQDNGTFQKHSQTSGHYGLTTIKQKLNLLGGSLVIQTYPRTEIAMMIPLESEEESNGKDRFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK54</accession>
    <name>H4GK54_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_8650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000059">
        <property type="protein sequence ID" value="EHS85688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122437.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000059.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8650"/>
    <dbReference type="EnsemblBacteria" id="EHS85688">
        <property type="protein sequence ID" value="EHS85688"/>
        <property type="gene ID" value="PS3_8650"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1298"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000150">
        <property type="entry name" value="Cof"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00099">
        <property type="entry name" value="Cof-subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS85688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="269" mass="29385" checksum="6D875B7953FF2E19" modified="2012-04-18" version="1">MAIKLVALDIDDTLLNSKGEILESTKKVISQALDQGIKVVLCSGRPLAGVKPYLDQLGIQGDDQYAVTYNGAIICSASGRILAKELLDNQQYRRLTAFGKEHQVPFNVLDEHSVIYTADHDIDFVTVVQAWENEAGIMVRQPDELPADFTITKGAFVGDPERLDQIQPLVETTFGDEMYVVRAGKCFLEMMNSKVNKGNALAQLSELLQIAPDEVMVFGDEGNDLAMFDFAGIAVCMGNGSDEAKAQADFVTASNDDDGIAQAFEKFIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK63</accession>
    <name>H4GK63_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="6">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_7755</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000059">
        <property type="protein sequence ID" value="EHS85697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7755"/>
    <dbReference type="EnsemblBacteria" id="EHS85697">
        <property type="protein sequence ID" value="EHS85697"/>
        <property type="gene ID" value="PS3_7755"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1308"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006268">
        <property type="term" value="P:DNA unwinding involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005751">
        <property type="entry name" value="ATP-dep_DNA_helicase_PcrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01073">
        <property type="entry name" value="pcrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="8">
        <location>
            <begin position="8"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="9">
        <location>
            <begin position="288"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="676"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="757" mass="85947" checksum="6B1509A4834F9B6C" modified="2012-04-18" version="1">MTEQSLLQGMNDKQQEAVLTTEGPLLVMAGAGSGKTRVLTHRIAYLIEEKHVLPWNILAITFTNKAAREMQERVGKLLGENARDVWVSTFHALCVRILRRDIDRIGFNRAFTIADSSEQRTLVKRICEDQNIDTQKFDPRSILSAISNAKNAMETPEQYLEKHRGPFGQMVGKVYQSYQTELANNQSLDFDDLIMKTIELFQTSSDTLAYYQDKFHYIHVDEYQDTNDAQYQLVNLLAQKYQNLCVVGDADQSIYGWRGANMNNILNFERDYPQAQTVMLEQNYRSTQTILKAANEVIINNLQRKDKNLWTENGAGEKISYYRAQNEHDEAQFIVKKIQEERANHQYHYNDFAVLYRTNAQSRVIEETFLKSSIPYTMVGGHKFYDRKEIRDILAYLTLIVNPQDSMSFTRVVNTPKRGIGDTSVEHLREFADDNHWPLLYAAQNVINANQIGAAARNKLDQFGQLMVDLTDQADQLSITDLTELILDKSGYDEALRQDKSLQAQARRENLDEFKSVTAEYDKQHADDNLPQMEKIINFLADLALVSDQDDVDEQEPAVTLMTLHAAKGLEFPVIFLIGMEQGIFPLARAMERTDEMEEERRLAYVGITRAKRKLYLTNAFSRMLYGRVQRNQPSDFIDEIDDSLIKTENEGGMVFGTGKGGLYGGYASQASQATTYQAKPRRTPGKKVAPKVANSGTGADQQAWQTGDKVTHKKWGTGTVVSVNGTGTDMELDIAFPQQGVKRLLASFAPITKVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK74</accession>
    <name>H4GK74_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative galactoside O-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_11223</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000061">
        <property type="protein sequence ID" value="EHS85635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122457.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000061.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS85635">
        <property type="protein sequence ID" value="EHS85635"/>
        <property type="gene ID" value="PS3_11223"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1325"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="185" mass="20097" checksum="40116575C10E8357" modified="2012-04-18" version="1">MDVYQRAAAGEPLDMRTDQEYHEVLGPEMVRSRTLCHRINMLDPYDPQVRDLFDDLFEGRLPQSSTILTPVQIDRGKTITIGEDTFINWGFNTVSTGGIHIGNHVQIGPNVALLTANHDLNDLQIIYCKPIVIEDGAWLGEGVKVTQGVTIGEGAIVAAGAVVTKDVPANAIVGGCPAKIIKDHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK85</accession>
    <name>H4GK85_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Malolactic regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_19586</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122468.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19586"/>
    <dbReference type="EnsemblBacteria" id="EHS85537">
        <property type="protein sequence ID" value="EHS85537"/>
        <property type="gene ID" value="PS3_19586"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1344"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="294" mass="33776" checksum="3D0A36A7A2CDD4D6" modified="2012-04-18" version="1">MNSRDLKYFIELVELRNYTQVAKAFKVSQPTITQAVQRLEKEFQTKLVNSERNHRDDMITRSGILLYENAKVMLGQLSTTHQEIERSKHRQIQIGVPPIIGKMVISQVVEKLQPQLLKRLKIISMGSHELLTEVTKGHLDLALIASTEPLHVSQITANPLAALPFKLIVSEHHPLAQYDQLSFAQLGQERFINFDQQYIHQLAFQTYCDYTGVKPSIAVYRIPDISWVKELVRQNYGISLMVEQAVRNEPGIKVIELTDEVPIKFYISLVIRQSYVMSPEEQGIVEDFKSIDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKD7</accession>
    <name>H4GKD7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Lysine transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_8617</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000063">
        <property type="protein sequence ID" value="EHS85501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8617"/>
    <dbReference type="EnsemblBacteria" id="EHS85501">
        <property type="protein sequence ID" value="EHS85501"/>
        <property type="gene ID" value="PS3_8617"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1428"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amoino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="439"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="AA_permease" evidence="7">
        <location>
            <begin position="16"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="476" mass="53140" checksum="5A7062C644D88824" modified="2012-04-18" version="1">MDQNQGEMKHSLKTRHLSMIALGGTIGTGLFIASGSAISTAGPGGALVAYLVMGLMVYFIMTSLAEMGTFNPISGSFTQYARDYVDPALGFAMGWNYWFCGAISVAVELSTVGIIMNFWFPHIASWWFSLGAFLLISLINYLTVNVYGETEFWLAFLKIAAVVIFVIVGALVIIGKLGHQGFVGFKFFHYRDAPFVNGFLGIVSAFVVSGFSFQGTEMVGIAAGESKDPKQSVPRAIHSTFWRILLFYVLTIFIIACLIPYDNKNLLSSDVTDIVTSPFTLIFHQAGLEYAATLMNFVILIAVLSSANSWLYAIIRILYSQGRSGDTWRGFGFVNRRGVPINSFLAMAGLSFLIWLIQFVGPQAYNYLIAASGLGGFMEWLGIALTHYRFRRAYLKQGYQLNDLPYRASLFPFGPIFAFILCIIVIVGQNVNAFITWDWQSILITYMSVPLFFIFMFYYKLRHHSHLIPLDQVKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKF0</accession>
    <name>H4GKF0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_18897</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000064">
        <property type="protein sequence ID" value="EHS85442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122532.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000064.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18897"/>
    <dbReference type="EnsemblBacteria" id="EHS85442">
        <property type="protein sequence ID" value="EHS85442"/>
        <property type="gene ID" value="PS3_18897"/>
    </dbReference>
    <dbReference type="GeneID" id="57060338"/>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1454"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="6">
        <location>
            <begin position="4"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="286" mass="31601" checksum="A5805484A4CEB145" modified="2012-04-18" version="1">MLPLIRRNLKLFFRNRAGVFFSLLGALISFGLFIIFLKSNMLDSWKQLQHPARVLDLWVIGGTLAVASITTTLSALSRMVSDLEYRVFDDLILTGLSRLKIRLSYVLAATIIGTLLQIALFLIMVSYFSWVDGISIPGYITFQVLIVTILASFSASVLNFILVYRMKNIDTVGKFSSIVGAASGFLVGIYIPIGVLPTFAQNLVKLTPGAYVAAIYRQLLMHDILKNVQPNILHNLQKTLGIGINLNGNLTTKLQNFAIVTGVTVILLIIILITNKHDQNKSVSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKF3</accession>
    <name>H4GKF3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA-3-methyladenine glycosylase I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_21107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000065">
        <property type="protein sequence ID" value="EHS85427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122535.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000065.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21107"/>
    <dbReference type="EnsemblBacteria" id="EHS85427">
        <property type="protein sequence ID" value="EHS85427"/>
        <property type="gene ID" value="PS3_21107"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1467"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005019">
        <property type="entry name" value="Adenine_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03352">
        <property type="entry name" value="Adenine_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS85427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="194" mass="22962" checksum="DB66D059DDFF5E62" modified="2012-04-18" version="1">MKTNLTRCSWANTDNALLRDYHDHEWGRPCHNNRMLFELLSLEIMQAGLSWQTVLNKRQALKAAFENFDYRTIQMLDSQLPQLLTNDKIIRNRRKILAIISNARIIDSLETQNKSFNQYLWDFIKWTPIQHHYQSHKDLPRTDQTARRISKQMKSDGFKFTGPVVIYSFMQAAGLVNDHEISCYRYKEITQTHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKG1</accession>
    <name>H4GKG1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_13380</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000066">
        <property type="protein sequence ID" value="EHS85396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13380"/>
    <dbReference type="EnsemblBacteria" id="EHS85396">
        <property type="protein sequence ID" value="EHS85396"/>
        <property type="gene ID" value="PS3_13380"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1480"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="17"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="163" mass="18146" checksum="85E6C0A57BC02D38" modified="2012-04-18" version="1">MKQAERRFIMEIKPKTFHKILVGVDNAPDALAAFDYAVDKAKRDGSELGIVSVYETHNVNVFQILDKDYVIDSREKLEEQVQEYVQAAIDYGVDPKKITLIVDQGDHPAERICNHVIPEFTPDLLVIGSIGKTGSRKAIGSQASYIVENSGVSVTVIRTDQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKG5</accession>
    <name>H4GKG5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flavodoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_12184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000067">
        <property type="protein sequence ID" value="EHS85379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122547.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000067.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12184"/>
    <dbReference type="EnsemblBacteria" id="EHS85379">
        <property type="protein sequence ID" value="EHS85379"/>
        <property type="gene ID" value="PS3_12184"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1489"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001226">
        <property type="entry name" value="Flavodoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12682">
        <property type="entry name" value="Flavodoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00201">
        <property type="entry name" value="FLAVODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="162" mass="18295" checksum="CCFBD604D4DED1B4" modified="2012-04-18" version="1">MTKTIVLYFSSTGTTKQLAEIIANKLATEMVELKALEPYSPADLNWHDEQARTTIEQHEHHSRVPVDPQTLPDLTGYDQVIIGHPIWWGIPPRLIADTIDHLDLNGKVLAPFATSGGSTYSRSQSFIERTIQENNYQTTVKTGQILHNTNDVDQWLKAINFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKG8</accession>
    <name>H4GKG8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Pyruvate carboxylase</fullName>
            <ecNumber evidence="2 8">6.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PS3_18590</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + hydrogencarbonate + pyruvate = ADP + H(+) + oxaloacetate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20844"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.4.1.1" evidence="2 8"/>
    <dbReference type="EMBL" id="AICN01000067">
        <property type="protein sequence ID" value="EHS85382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122550.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000067.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18590"/>
    <dbReference type="EnsemblBacteria" id="EHS85382">
        <property type="protein sequence ID" value="EHS85382"/>
        <property type="gene ID" value="PS3_18590"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1493"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006090">
        <property type="term" value="P:pyruvate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003379">
        <property type="entry name" value="Carboxylase_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005930">
        <property type="entry name" value="Pyruv_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02436">
        <property type="entry name" value="PYC_OADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001594">
        <property type="entry name" value="Pyruv_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01235">
        <property type="entry name" value="pyruv_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0092">Biotin</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="14">
        <location>
            <begin position="1"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="13">
        <location>
            <begin position="121"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="15">
        <location>
            <begin position="531"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="12">
        <location>
            <begin position="1073"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="540"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="612"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="11">
        <location>
            <position position="709"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="738"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="740"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="872"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR001594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EHS85382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="1143" mass="126825" checksum="59BB6C87ADB178CC" modified="2012-04-18" version="1">MFKKVLVANRGEIATRIIRACHELGIKAVAVYPKEDEFSAHRQAADEAYLIGAGQAPIDAYLNIDEIIRVAKLSGAEAIHPGYGFLAENADLAQACVDNGIKFIGPKPEHLRMFGDKLTAKQVSIDAGLQPIPGLVGDIDDVDQVREFAHQHGYPIMIKAANGGGGRGMRIINDDQELVAEYDQARDEAMKAFGSSKMYVEKDIQNPKHIEVQIIADEHGHVMHLFERDCSVQRRHQKVIEFAPSVVLSPQRRAQVCQMAVKLMESVHYQNAATVEFLETPEQFYFIEVNPRIQVEHTVTELITGIDLVKTQLQVAAGEDLHADIHLPQQADLKYNGAAIQCRVTTEDPANNFMPDTGKITTYQAPGGPGVRLDGNVYPGYQVTEFFDSLLVKCCTKGEDFDDARVKMVRALQEFTVRGVKTNIPFMENVLQDPTFKQGDCSTTFIDETPGLFNFENQTDAKAQLVNYISNVTVNGFAGVEQRPELTLPEMNAPRLDQIEPTTPTPRTVKDILDAEGAEAALAFVKEQPKVLLTDTTMRDAHQSLFATRMRTYDMLPAMKVYDQALPQLFSVESWGGATFDVCYRFLGEDPWVRLRLLKEYMPHTLQQMLLRASNSVGYQNYPDNVLKRFIDHSAKSGVDVFRIFDSLNWIEQMQRSIDYVRETGKIAEGAMCFTGDFLNPNEHKYTLNYYLDLAQSLIDAGAQMIGIKDMAGLLKPQAAYELVGELKAKFDVPIHLHTHDTTGNGVATYVSATQAGVDIVDVAMAAMAGTTSQPSLGTFYYALEGDERQPALNMNNVARLNNYWANVRPLYQDFSNGVNAPQPDLYQTEMPGGQYSNLQQQSKSLGINDFSKVEAKYREVNQLLGNIIKVTPSSKVVGDMAIFMIQNQLDKDNILTKGQQVDFPDSVVNFFAGDLGQPYGGFPADLQKVVLKDHPAITVRPGSLADPVDFAAVKAELAEKIHREPTEDDLLGYLMYPKVFVDYFHNNEQYGPLMDLDTTTYYQGMRPGEEIQIQLSAGQTMLLKLLFVTPADADGYRTLYYELDGQAYQMSVKDQSLKGQVVSVPKADPDDPSQIGMPLNGTVVKVNVKAGDQVKVGDVLVTTEAMKMESAVKAPFTGTVQEVLVKVGDTLKSQDLLLTLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKI3</accession>
    <name>H4GKI3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_21469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122565.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21469"/>
    <dbReference type="EnsemblBacteria" id="EHS85263">
        <property type="protein sequence ID" value="EHS85263"/>
        <property type="gene ID" value="PS3_21469"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1524"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025536">
        <property type="entry name" value="DUF4422"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14393">
        <property type="entry name" value="DUF4422"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DUF4422" evidence="1">
        <location>
            <begin position="4"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="253" mass="29997" checksum="20A4343EAF00E22E" modified="2012-04-18" version="1">MKIKILVATHKNYQMPTDPVYLPVAVGQALHPDRQLPYQGDDQGENISVKNPHYNELTAIYWGWKNLDVDAMGLVHYRRHFMSRPGRKMQDVISGAEVAALLEQYDVLLPKRRHYWIETNRSQYLHAHEHAPFETMEKVIKRDYPEYGAAFDLVMQRTWGHMFNMFIMKKEPLDQFSQWMFDVLGKVEAQTDVSTFTPYEQRVYGFLSEHLLDIWLEVHAEYRVKEIPYAFMESTNWFKKGGNFLKRKLIGHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKM1</accession>
    <name>H4GKM1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9 11">Uracil-DNA glycosylase</fullName>
            <shortName evidence="9">UDG</shortName>
            <ecNumber evidence="4 9 11">3.2.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ung</name>
        <name evidence="13" type="ORF">PS3_11868</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9 11">Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9 11">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9 11">Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="4 9 11"/>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122603.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11868"/>
    <dbReference type="EnsemblBacteria" id="EHS85190">
        <property type="protein sequence ID" value="EHS85190"/>
        <property type="gene ID" value="PS3_11868"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1578"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10027">
        <property type="entry name" value="UDG-F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00148">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002043">
        <property type="entry name" value="UDG_fam1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018085">
        <property type="entry name" value="Ura-DNA_Glyclase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264">
        <property type="entry name" value="PTHR11264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="SSF52141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00628">
        <property type="entry name" value="ung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00130">
        <property type="entry name" value="U_DNA_GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 9 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 9 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDG" evidence="12">
        <location>
            <begin position="50"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9 10">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS85190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="228" mass="25735" checksum="4213B24E8730866F" modified="2012-04-18" version="1">MQELIHNDWWPVLKNVFEAPAYQQLRDFLINEYQTQTVYPPAEDIFNAFRWTPYSQVKVVILGQDPYHGPGQAHGCSFSVRPGVPLPPSLKNIYQELTTDVGAQPVHHGYLKSWADQGVLLLNAVLTVRSGQANSHRGMGWETITDAAIQALSQRPQPVVFILWGRSAQAKERLIDLNRNYVITSAHPSPLSAYRGFFGSRPFSRANLALQSWGVEPIDWQLPMNVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKN3</accession>
    <name>H4GKN3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter, CydDC cysteine exporter (CydDC-E) family, permease/ATP-binding protein CydD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_11872</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122615.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11872"/>
    <dbReference type="EnsemblBacteria" id="EHS84953">
        <property type="protein sequence ID" value="EHS84953"/>
        <property type="gene ID" value="PS3_11872"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1593"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042883">
        <property type="term" value="P:cysteine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014216">
        <property type="entry name" value="ABC_transptr_CydD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02857">
        <property type="entry name" value="CydD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="22"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="332"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="365"/>
            <end position="372"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS84953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="575" mass="64838" checksum="22846E81DE562D32" modified="2012-04-18" version="1">MIDRLMFKLTGIRQLMGKLVGLYVLQAFLVIGQSLFLSYFLVRLWRGHQVLDEWPWLAWFVGCYLARHALNWLASRWLDDYARQTVRQMRQSLLAKLFVLGPQVVQREGTGNMVTLALDGISQVEEYITLVFSKMINMMIAPILILIVTFYLDLTSGIVMVIVYPLIILFFIILGLAAQARADRQFETFQLLSNHFIDSLRGIDTLKYFGLSKPYSKSIYRSSENFRLSTMATLRIAMTSTFALDFFTTLSIAMMAVFLGFKLIDGEIGLFPALGILIMAPEYFLPLRNFANDYHATLNGKNSFQKIMAIINEPSPKTSSAKLHSWSASDQLQVDQVAFQYGEDGAQLKPLSFTARGYQKIGIIGMSGSGKTTLINLLSGFLQAKAGQFVVQGQAMSTMDESTWREQITYIPQSPYLFDDTLRNNIAFYTPDASVEQIQEAVKVVGLTDLVNELPEGLDTMIGTGELALSGGQAQRIALARAFLDQSRKVMIFDEPTAHLDLETEIALKEAMLPLMKDHLVFFATHRLHWMRQMDYILVMDHGQLVEQGTYDELMAHQGSFVKLVNQMKGGETDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKP4</accession>
    <name>H4GKP4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha/beta fold family hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_9428</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122626.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9428"/>
    <dbReference type="EnsemblBacteria" id="EHS84964">
        <property type="protein sequence ID" value="EHS84964"/>
        <property type="gene ID" value="PS3_9428"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1614"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="32"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS84964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="247" mass="27738" checksum="A362E1C32A51EBE6" modified="2012-04-18" version="1">MEKDIIIKRDGLNLAAKVSFPDQDQYDVAILAYGFVGMMDPKVNDLLPVLAQKLQALGIGTVRFDFNGHGLSDGPLARMSIYNELEDYEAIMEYVLELPQLRHLYLIGHSQGGVLSAMMAGFYPDKVDKLVLMSSAATLVDDAKLGTCMGVDYDPDKVPAELDFGDFKVNGWYFRTAKFINTYEVAANYQGPVLIIHGQDDQIVNNYASTRFHAIYKHSELHLIPESDHGLHQNRDEVYQLVTNFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKQ6</accession>
    <name>H4GKQ6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative pyruvate dehydrogenase E1 component, alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_20085</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20085"/>
    <dbReference type="EnsemblBacteria" id="EHS84976">
        <property type="protein sequence ID" value="EHS84976"/>
        <property type="gene ID" value="PS3_20085"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1629"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016624">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="E1_dh" evidence="3">
        <location>
            <begin position="18"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS84976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="324" mass="35299" checksum="B1A638965CD412CB" modified="2012-04-18" version="1">MEVEGITSEQAQVMYKTMWNIRNFEENARRFFAAGQIPGFVHLYAGEEAIATGVCANLTDRDYITSTHRGHGHCVAKGGDLKGMMAEIFGRSTGLGKGKGGSMHIADLDKGILGANGMVGGGFGLATGAAMRNKYLHTNDVAVCFFGDGAANEGNFHECLNMASIWKLPVIFVNENNLFAESTPQWYSSGSGTIAERAAAYNMPGVRVNGKDIIAVYEVTKDAVERARNGEGPTLIEAVTYQNYGHFEGDEQKYKAPDGEEKAWADVDPMQVFYDYAIEHELLTAEELDQLAQESEQAVKEAIEFAQNSPIPEPESLLDDVFAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKR3</accession>
    <name>H4GKR3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Isoprenyl transferase</fullName>
            <ecNumber evidence="2">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_8972</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8972"/>
    <dbReference type="EnsemblBacteria" id="EHS84983">
        <property type="protein sequence ID" value="EHS84983"/>
        <property type="gene ID" value="PS3_8972"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1636"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="PTHR10291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="SSF64005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="16"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="60"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="192"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS84983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="241" mass="27060" checksum="28542FF0AE0BB680" modified="2012-04-18" version="1">MDLERIPNHIAIIMDGNGRWAQARHLPRVAGHQQGMQTVKTITKAASDLGVKVLSLYAFSTENWKRPGAEVKFLMQLPIKFFNTFVPDLVKNNVQVRVMGDISQLPKATQKAVNDAIADTAACDGMILNFALNYGGRDEILRATKYLAGQVASDELTIDDLDEDILSQAMMTGSLGEYADPELLIRTSGEERISNFMLWQLAYSELVFVDEHWPDFDGESLRRSIAVYQNRHRRFGGLVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKR9</accession>
    <name>H4GKR9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_21280</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0213 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21280"/>
    <dbReference type="EnsemblBacteria" id="EHS84989">
        <property type="protein sequence ID" value="EHS84989"/>
        <property type="gene ID" value="PS3_21280"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1645"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="SSF82771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIY-YIG" evidence="2">
        <location>
            <begin position="5"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS84989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="94" mass="11050" checksum="116E99A149847175" modified="2012-04-18" version="1">MTESKPYFMYVLLCADKTFYCGFTDDVQRRLATHEAGKGAKYTRPAKRHPLKLLYQAEFATKQAALQAEYQFKHLRRDQKEEFLREHGVNGFTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKS0</accession>
    <name>H4GKS0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_18734</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122652.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18734"/>
    <dbReference type="EnsemblBacteria" id="EHS84990">
        <property type="protein sequence ID" value="EHS84990"/>
        <property type="gene ID" value="PS3_18734"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1646"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTS" evidence="1">
        <location>
            <begin position="35"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="251" mass="28214" checksum="1EF7C4605FB12CDC" modified="2012-04-18" version="1">MQVPLQADERIDQLSSQGVQIIQSSQVFAFSLDAVLLAHFVPENHRQHLKTVDLCAGNGAVGLFLNQHLGGQIWEVELQARLADMALRSINLNGLGDRYHVINDDLANVYDYLPKDSVDIVTCNPPYFKDDQHSQKNPNQYLAIARHEIKTNLATVVDRMSGLLKMNGHAYMVHRPDRLTEILSTMEASRLVPKRLQFICPKPQWEANMVLIDAIKDGRPGGLKVLPEFMVADENGEYQPAMKELLYGDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKS8</accession>
    <name>H4GKS8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dipeptidase</fullName>
            <ecNumber evidence="5">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_6380</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an L-aminoacyl-L-amino acid + H2O = 2 an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:48940"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="ChEBI" id="CHEBI:77460"/>
            <dbReference type="EC" id="3.4.13.19"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the peptidase C69 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122660.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="MEROPS" id="C69.001"/>
    <dbReference type="EnsemblBacteria" id="EHS84998">
        <property type="protein sequence ID" value="EHS84998"/>
        <property type="gene ID" value="PS3_6380"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1656"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070004">
        <property type="term" value="F:cysteine-type exopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016805">
        <property type="term" value="F:dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005322">
        <property type="entry name" value="Peptidase_C69"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12994">
        <property type="entry name" value="PTHR12994"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03577">
        <property type="entry name" value="Peptidase_C69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS84998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="468" mass="52665" checksum="72B84AE64ED333D2" modified="2012-04-18" version="1">MNELSACTSILIGKNATIDHSIIIGRNEDSKSSWPKHFIVHPHGGLPAEFISKDTGVRIPLPQPSYRYSATPEWTDQAGLFEEDGINEFDVAMSATESAYSNDLVLSADPYVENGINEEAMVTLVLPFVKSARAGVQRLGDLIAQYGTGETNGILFADNEEAWYMEIGSGHYWVAQRIPDNAYAVAGNQLAIQEIDFNDSTNFMYHPEIVNFVNQHQLNPHPGTFNFRQIFGTATPADAIYSTPRVWYGHHLFSPQAAKDEHPEDQNLPFIMRPEKRLSILDAQKYLNSHFQGTIYDPLNGPDQVASRRYRPISLAKTQESHLLQMNRPSANLHWLSMGVGAQSNFIPFYNGLTQTLPAYQKGSLPASLDSAYWVYKHVGVLIDHNLKIGWPLLNEVQQTVTTQALTFIHEMDQLLNTTPIEEHSSLINERNNQFAAWTLEQYQKLAMRLLTDLTDDSPLNFNTDDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKS9</accession>
    <name>H4GKS9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Penicillin-binding protein (Beta-lactamase class C)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_12102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12102"/>
    <dbReference type="MEROPS" id="S12.A21"/>
    <dbReference type="EnsemblBacteria" id="EHS84999">
        <property type="protein sequence ID" value="EHS84999"/>
        <property type="gene ID" value="PS3_12102"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1657"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="349" mass="38361" checksum="2B3D59F4F467204F" modified="2012-04-18" version="1">MVGFGCWEQAQAASTKTIKQTINQLVKQKGFSGTITVVKNGRVIIQTSRGYANYAKKQPNRSQNQYQIASIQKSMTAEMIAKLASQKKLTLNDKLSKYYPKIKNADQVTLRQLLNMVSGLKVSSTISLHSFKSQQAYLNYLGSHATIQTNKIGQWQYSSFNYNLLAGIVEQVSGKSYQQYFKQVIAKPLGIKNYSFVTKFSSNVAQGYYNRGLSHYQAAGKLGKTNQYMSFGAGTIAMSGQDLYKVLAAMLSGKLIGTGNAQDLYSSKYLSGTTYTAGFYHVGQQLIKNQATYHMHGRQSGVDTAVEISADGQNAVIVQSNNGTLTSGGTINHVIDLPIYKKLVGYDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKV1</accession>
    <name>H4GKV1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histidinol-phosphate aminotransferase</fullName>
            <ecNumber evidence="5">2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hisC</name>
        <name evidence="7" type="ORF">PS3_12416</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2-oxoglutarate + L-histidinol phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122683.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12416"/>
    <dbReference type="EnsemblBacteria" id="EHS85021">
        <property type="protein sequence ID" value="EHS85021"/>
        <property type="gene ID" value="PS3_12416"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1680"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00012"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 5 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="6">
        <location>
            <begin position="29"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="357" mass="40500" checksum="F5C4B464364D320B" modified="2012-04-18" version="1">MKESIKHLKSYEPETSVEEVKAEYHLDRIVRLSANENPYGTSPKVKEAILNWDFDGNYYPDSESKILRNQIANYWSLSPDQLIFGVGLDEILELISRTFLTPGDEVVVPDPTFSEYALHAEIEGAKVVKVPIDAETGHYDWAAMTKAVTQQTKLVWLCNPNNPTGVIESRADIQAFLDQVSTDTLVLVDEAYMDFADEAQGVTCFPLLSQYPNLAVLRTFSKAYGLAGFRVGFIAMAPQLAQYMQMIRLPYNIPTVSQIAAQAAFADQAFVDQVVAKNRVERQRWMDFFDQMGVKYFPSQTNFIYYQVDDEQGLAEALLSQGYQIRRGFQPHWLRNTIGLPADNDQMMAITREFLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKW6</accession>
    <name>H4GKW6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UPF0398 protein ypsA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_12886</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122698.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12886"/>
    <dbReference type="EnsemblBacteria" id="EHS85036">
        <property type="protein sequence ID" value="EHS85036"/>
        <property type="gene ID" value="PS3_12886"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1702"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010697">
        <property type="entry name" value="YspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38440">
        <property type="entry name" value="PTHR38440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06908">
        <property type="entry name" value="YpsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS85036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="198" mass="23544" checksum="7C686DF283BB133B" modified="2012-04-18" version="1">MKRVWISGYRAYELGVFQEDDPKQLVIQTVLSQLIQDRLNQSNEETWVISGPQMGTERWGLKSAIALKDDYEQLRVSMLLPFADFGQNWNEDNQLRLSAIREQMDYQGTVSNQPYQGPQQLRNYQRFMFEHSDELILVFDPEQELNPDQHSKPFWDYRAAQKYQETQPTYQITLVTMDQLQDAAEEWAQRQVEEHDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL13</accession>
    <name>H4GL13_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell surface hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_21317</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122745.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21317"/>
    <dbReference type="EnsemblBacteria" id="EHS84779">
        <property type="protein sequence ID" value="EHS84779"/>
        <property type="gene ID" value="PS3_21317"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1761"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="2">
        <location>
            <begin position="94"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS84779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="320" mass="36678" checksum="3042864DCBCC7290" modified="2012-04-18" version="1">MPSEKQKLMLSGLATVLGAAGISFLVSEKLFKIAFQRVNYVPETSADKQKYADLYWDYVAWFKKVNKEHWTFSMDDDDDLMSAYFIPAKEPSLKAVVISHGYKGNGETMANYAKMFYELGFNVLLPDDRGHGQSAGSYISFGWLDRIDYQTWLHKLITRLGDQVKIVLFGVSMGGATVEMLSGDELPAQVKCIIADCGYSSIEDELQYLLKRQYHLPAHPFLPMVSTINRHRLGYYLKDVSSTNQLKKNTRPIFFIHGEKDVYVPSWMALENYQATEAPKKLWLVNQATHAESFWINPVDYQDHITEFLQQYFGNSTIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL14</accession>
    <name>H4GL14_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase</fullName>
            <ecNumber evidence="2">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_21709</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122746.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21709"/>
    <dbReference type="EnsemblBacteria" id="EHS84780">
        <property type="protein sequence ID" value="EHS84780"/>
        <property type="gene ID" value="PS3_21709"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1764"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="3">
        <location>
            <begin position="34"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS84780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="391" mass="42397" checksum="4900973F7EDB54D7" modified="2012-04-18" version="1">MPHSNLAIRYNYHLDEIKPNNILTFNQEISSIPDIVKLTLGEPDFNVPEIGKQAAIASIENNDSHYAPGTGSVALRKAIADFLKVRYQLTYSPDQEIVVTIGATEAIYASLNALLNPGDKVLIPTPTFPLYETVVRMLGGVPINLDTSSNNFVLSPAMVKAALDEYGPAVKAVILNYPSNPTGVTYSASDLQALAEVLAQSKAVVIADEIYSELLYDGQHVSIATYLPEQTLVLNGASKSHAMTGYRIGFIAGPTEIMQKVSLVHNLMITSPSDPAMAAAVPCFGTETGRQASVEMRAAYQIRRDYMVEELTNLGFTVAKPNGAFYIFAQIPRQFGSDDVQFARDLAQEAQVATIPGSFFGPGGEGYLRMSYATSLDDLKTAISRIQNFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL30</accession>
    <name>H4GL30_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator TetR/AcrR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_13418</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122762.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS84660">
        <property type="protein sequence ID" value="EHS84660"/>
        <property type="gene ID" value="PS3_13418"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1782"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039532">
        <property type="entry name" value="TetR_C_Firmicutes"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14278">
        <property type="entry name" value="TetR_C_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="7"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS84660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="189" mass="21781" checksum="87206B3DD7733C55" modified="2012-04-18" version="1">MTSKRNTKTKAKIERAFLQLMEDKGFNQLTVKDITQLAQINRSTFYLNYADKYDLLAKIEAKLFQNIHQILKLDKTNSYFDEASILAVLNYALANHRLVKVLTEGDLASQVESLLKQEIIAIVEEQLHVNFNQDPIVNSPYAREIFASSISAIIILWIKRDFQESVEEIMQILESLRQYSLGQIINEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL31</accession>
    <name>H4GL31_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Adenine phosphoribosyltransferase</fullName>
            <shortName evidence="10">APRT</shortName>
            <ecNumber evidence="5 10">2.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">apt</name>
        <name evidence="12" type="ORF">PS3_8648</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="5 10"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122763.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8648"/>
    <dbReference type="EnsemblBacteria" id="EHS84661">
        <property type="protein sequence ID" value="EHS84661"/>
        <property type="gene ID" value="PS3_8648"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1785"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00646"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006168">
        <property type="term" value="P:adenine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00004">
        <property type="entry name" value="Aden_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005764">
        <property type="entry name" value="Ade_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01090">
        <property type="entry name" value="apt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="11">
        <location>
            <begin position="31"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS84661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="175" mass="18943" checksum="99510426AF89D8D9" modified="2012-04-18" version="1">MTVNLYDHVESIPDFPEKGIVFRDILPLMANGEAFKQATAELAQFARERGVEMVVGPEARGFIVGCPIAYDLGVGFAPARKKGKLPRPAVSATYDLEYGSATLQMEHDAIKPGQKVLVVDDLLATGGTILATIDLVEQMGGIVVGCAFLVDLKDLHGMERLKDYDVKALMEFSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL35</accession>
    <name>H4GL35_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GTPase Obg</fullName>
            <ecNumber evidence="7">3.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">GTP-binding protein Obg</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">obg</name>
        <name evidence="11" type="ORF">PS3_5402</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122767.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5402"/>
    <dbReference type="EnsemblBacteria" id="EHS84665">
        <property type="protein sequence ID" value="EHS84665"/>
        <property type="gene ID" value="PS3_5402"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1789"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01898">
        <property type="entry name" value="Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.210.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01454">
        <property type="entry name" value="GTPase_Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014100">
        <property type="entry name" value="GTP-bd_Obg/CgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036346">
        <property type="entry name" value="GTP-bd_prot_GTP1/OBG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015349">
        <property type="entry name" value="OCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="PTHR11702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09269">
        <property type="entry name" value="DUF1967"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002401">
        <property type="entry name" value="GTP_bd_Obg/CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102741">
        <property type="entry name" value="SSF102741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="SSF82051"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02729">
        <property type="entry name" value="Obg_CgtA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03595">
        <property type="entry name" value="Obg_CgtA_exten"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51881">
        <property type="entry name" value="OCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Obg" evidence="10">
        <location>
            <begin position="2"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="8">
        <location>
            <begin position="161"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="OCT" evidence="9">
        <location>
            <begin position="358"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="167"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="192"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="214"/>
            <end position="217"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="284"/>
            <end position="287"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="318"/>
            <end position="320"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS84665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="436" mass="47584" checksum="AF57ABF84B93D338" modified="2012-04-18" version="1">MPTFVDQIKIEVHAGRGGNGMVAFRREKYVPNGGPAGGDGGRGGSIILKVDEGLRTLMDFRYHRIFKAKNGQNGMSKQMTGANAQDTVIAVPQGTTVRDLTTGQLIGDLVESGQELVIAKGGRGGRGNMRFATAKNPAPEIAENGEPGESREIELELKMLADVGLIGFPSVGKSTLLSVVTGAKPKIAAYEFTTLVPNLGMVMLPDGRDFAMADMPGLIDGASKGVGLGLQFLRHIERTRVLLHLVDMGSQDPDQAIERYHAINHELATYDPELLKRPQIVVATKMDLPDASENLKRFETMLATDDTLAQQPEVFAISAVTHAGVQELMQTTANLLEVTPEFDSNSHDEQMVVEYTAPSEDQEPSFTISRDDDGTWVLGGAKLERLFAMTNLDHEESQMRFARQLRGMGVDEALRERGIQSGDLVRIEDFVFEFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL36</accession>
    <name>H4GL36_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UvrABC system protein C</fullName>
            <shortName evidence="7">Protein UvrC</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Excinuclease ABC subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">uvrC</name>
        <name evidence="11" type="ORF">PS3_9431</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with UvrB in an incision complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0213 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the UvrC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122768.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9431"/>
    <dbReference type="EnsemblBacteria" id="EHS84666">
        <property type="protein sequence ID" value="EHS84666"/>
        <property type="gene ID" value="PS3_9431"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1790"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00203">
        <property type="entry name" value="UvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004791">
        <property type="entry name" value="UvrC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001162">
        <property type="entry name" value="UvrC_RNase_H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038476">
        <property type="entry name" value="UvrC_RNase_H_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08459">
        <property type="entry name" value="UvrC_HhH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="SSF46600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="SSF82771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00194">
        <property type="entry name" value="uvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50165">
        <property type="entry name" value="UVRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 7" id="KW-0228">DNA excision</keyword>
    <keyword evidence="6 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 7" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="GIY-YIG" evidence="9">
        <location>
            <begin position="15"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="8">
        <location>
            <begin position="197"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="UVRC" evidence="10">
        <location>
            <begin position="248"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS84666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="603" mass="68789" checksum="97A4D8D291A8DCE4" modified="2012-04-18" version="1">MASEYLEHKLALLPDRPGCYLMKNINGQIIYVGKAKNLKNRVRSYFKSHHDGKVAKMVSEVADFETIITATNKESFLLEVTLIQKHKPYFNILLKNGSGYPYLKITSGKDPELKITSQILNDGAYYFGPYPNVYAAQETLHFLWKVYPLKRCHGHQGRPCLYYHMGQCLGGCFREVPQAEYDAQIKKIKSFLNGNTAAVKKDLETQMQTASANLEFERAAEIRDLIHYIQVTVEKQKIISNDKTPRDIINFYLDKGWLSLQIFFVRQSRLMKRVKRLFPVVDDAEDEMTAFILQFYQQKNQLLPKELLLPATLPTKEISEILGIPVRTPQRGKNRDLMNMALDNAKISLDEKFRLLGMDSQKTTGAMKEITDALGLPAGHKIEAFDHSHIQGSDPVSAMVVFVNGEPAKKLYRKYKLTTVIDHADETASTKEVIRRRYTRLLKEGETMPDLIMMDGGEIQMNAARDVLENELDLDIPVVGMVKNDKHKTADLLFGDDDHHINLDPRSQGFYLVQRIQDEVHRFAINFHRNVRTKHSLSSRLDEIKGVGPRTRNKLMKEYGSINKIAQATVEDIQSLGINHATAQLIKVSLQKQAEVAKESSHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL57</accession>
    <name>H4GL57_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide deformylase</fullName>
            <shortName evidence="2">PDF</shortName>
            <ecNumber evidence="2">3.5.1.88</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Polypeptide deformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">def</name>
        <name evidence="3" type="ORF">PS3_7138</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + N-terminal N-formyl-L-methionyl-[peptide] = formate + N-terminal L-methionyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:24420"/>
            <dbReference type="Rhea" id="RHEA-COMP:10639"/>
            <dbReference type="Rhea" id="RHEA-COMP:10640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:49298"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="EC" id="3.5.1.88"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2">Binds 1 Fe(2+) ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the polypeptide deformylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.88" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122789.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7138"/>
    <dbReference type="EnsemblBacteria" id="EHS84687">
        <property type="protein sequence ID" value="EHS84687"/>
        <property type="gene ID" value="PS3_7138"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1814"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042586">
        <property type="term" value="F:peptide deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00487">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00163">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023635">
        <property type="entry name" value="Peptide_deformylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036821">
        <property type="entry name" value="Peptide_deformylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10458">
        <property type="entry name" value="PTHR10458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01327">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004749">
        <property type="entry name" value="Pep_def"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01576">
        <property type="entry name" value="PDEFORMYLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56420">
        <property type="entry name" value="SSF56420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00079">
        <property type="entry name" value="pept_deformyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="2">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS84687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="188" mass="21153" checksum="199FEE12CCCF8D2D" modified="2012-04-18" version="1">MITMDDIVRDTDNNPVLRTRAAKVDFPLSAENQQLAQQLMEYLENSQNEEIAEKYQLRAGVGLAAPQVNHGVRMTAVLIPGENEEDEPAFKDVLVNPVIISESVQMAALAEGEGCLSVDRDVPGYVPRHDRITLKYQNLAGETKQVRLKHYPAIVVQHELDHLIGVLFYDHIDENEPFNHPDDLMLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL75</accession>
    <name>H4GL75_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase</fullName>
            <ecNumber evidence="10">2.4.1.227</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">murG</name>
        <name evidence="13" type="ORF">PS3_1418</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + UDP-N-acetyl-alpha-D-glucosamine = beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + UDP</text>
            <dbReference type="Rhea" id="RHEA:23192"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:60032"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="EC" id="2.4.1.227"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 28 family. MurG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.227" evidence="10"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122807.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_1418"/>
    <dbReference type="EnsemblBacteria" id="EHS84705">
        <property type="protein sequence ID" value="EHS84705"/>
        <property type="gene ID" value="PS3_1418"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1838"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051991">
        <property type="term" value="F:UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol 4-beta-N-acetylglucosaminlytransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050511">
        <property type="term" value="F:undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030259">
        <property type="term" value="P:lipid glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00033">
        <property type="entry name" value="MurG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006009">
        <property type="entry name" value="GlcNAc_MurG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01133">
        <property type="entry name" value="murG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="1 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0133">Cell shape</keyword>
    <keyword evidence="9 10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_transf_28" evidence="11">
        <location>
            <begin position="4"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_tran_28_C" evidence="12">
        <location>
            <begin position="189"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-N-acetyl-alpha-D-glucosamine binding" evidence="10">
        <location>
            <begin position="10"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS84705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="368" mass="40269" checksum="04698DFB7BCA291E" modified="2012-04-18" version="1">MKLMVAGGGTGGHIYPALALIERLKQIEPTTQVLYIGTTRGLENKIVPQAGLELRTMEMQGFKRSLSVSNLKTIYLFLKSVQTAKKWLKEFQPDVVLGTGGYVSGAVLYAAAKMGIPTVIHEQNSVVGVTNKFLSKYVDEIAISFQAARSQFPSDKVTLTGNPRAQQVFMHEQTAFSWTADGLQDQVPTLMIFGGSQGAPKINQSVVEAIPEFNQRHYQVIFATGQKRYDQVMQDLAGVTLSNNVKVVPYIEDMQKKLPHVDVLVSRAGATTIAEVTALGLPTIMIPSPYVTANHQVKNAQALVDKGAAEMILEDQLTGKSLLDAADRLMLDERLRRQMAGKSAKLGHPKAADELITVLNQAIQKHQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL79</accession>
    <name>H4GL79_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA small subunit methyltransferase H</fullName>
            <ecNumber evidence="6">2.1.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">16S rRNA m(4)C1402 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">rRNA (cytosine-N(4)-)-methyltransferase RsmH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rsmH</name>
        <name evidence="7" type="ORF">PS3_3029</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine(1402) in 16S rRNA + S-adenosyl-L-methionine = H(+) + N(4)-methylcytidine(1402) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10286"/>
            <dbReference type="Rhea" id="RHEA-COMP:10287"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74506"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the methyltransferase superfamily. RsmH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.199" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122811.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3029"/>
    <dbReference type="EnsemblBacteria" id="EHS84709">
        <property type="protein sequence ID" value="EHS84709"/>
        <property type="gene ID" value="PS3_3029"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1843"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071424">
        <property type="term" value="F:rRNA (cytosine-N4-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01007">
        <property type="entry name" value="16SrRNA_methyltr_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002903">
        <property type="entry name" value="RsmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023397">
        <property type="entry name" value="SAM-dep_MeTrfase_MraW_recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265">
        <property type="entry name" value="PTHR11265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01795">
        <property type="entry name" value="Methyltransf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004486">
        <property type="entry name" value="MraW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81799">
        <property type="entry name" value="SSF81799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00006">
        <property type="entry name" value="TIGR00006"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="6">
        <location>
            <begin position="34"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS84709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="315" mass="35011" checksum="A4862395111854A9" modified="2012-04-18" version="1">MTQFNHVTVLLHEAVDGLNIKPAGIYVDGTLGGGGHSQAILERLSSEGHLYAFDQDQTAIQYNQEHLKQYIDDGRLTLVKSNFSQLKVALAQAGVSTIDGMVYDLGVSSPQFDDAKRGFSYQHDAPLDMRMDQTQSLSAMEVVNEWSFQDLMRILSRYGEEKFAKQIARSIEKQRQAAPIKTTLELAEIVKNAISAPARRHGGHPAKKSFQAIRIAVNNELGVLESSLEQALDLLTVGGRISVITFQSLEDRLVKTMFKERTTIPDLPAGLPVIPKETQPDFKLVNKKPIIASDQELATNHRAHSAKLRIIERIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLA1</accession>
    <name>H4GLA1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribonuclease Y</fullName>
            <shortName evidence="9">RNase Y</shortName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rny</name>
        <name evidence="12" type="ORF">PS3_6528</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Endoribonuclease that initiates mRNA decay.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the RNase Y family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6528"/>
    <dbReference type="EnsemblBacteria" id="EHS84600">
        <property type="protein sequence ID" value="EHS84600"/>
        <property type="gene ID" value="PS3_6528"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1877"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00335">
        <property type="entry name" value="RNase_Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017705">
        <property type="entry name" value="Ribonuclease_Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022711">
        <property type="entry name" value="RNase_Y_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF15">
        <property type="entry name" value="PTHR12826:SF15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12072">
        <property type="entry name" value="RNase_Y_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="SSF54791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03319">
        <property type="entry name" value="RNase_Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="HD" evidence="11">
        <location>
            <begin position="326"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="53"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS84600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="510" mass="57115" checksum="580C00BC3614C0C3" modified="2012-04-18" version="1">MIAIVAGGGIGYMIRKSMFEKRIMRAHVSAEGIITAAKKEAETAKKEAILEAKEESHRYREGVEQELKGRRQDVQKQEERLLQRENSLDRKDNALENRENAVSKREQKLDRRTEKVERDEQQVAKLVDQQAQELQRIAGLTNEEAKEQLFDQLNHQLAGEREKLIRESETEAQYTADKKAKGLIVEAIQRSAADMVSEATVTVVNLPNDDMKGRIIGREGRNIRTIETLTGIDLIIDDTPEAVILSGFDPVRREIAKIALDRLIKDGRIHPARIEEAVDKAREEMDAHLREVGEQTVFDLGIHGLHPDLIKTIGRMKYRTSYGQNVLQHSIEVAKLMGILAAELGEDVALAKRAGLLHDIGKAIDHEVEGSHVELGVELTQKYQENPVVINAIASHHGDVPAESVIAVLVQATDAISGARPGARSESLEQYIQRLTKLEALANEHEGVKNSYAIQAGREIRVMVQPNKVSDKQSAALAFDLKNEIEQQLEYPGHIKVTVIRETRSIEYAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLA2</accession>
    <name>H4GLA2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6 7">Protein RecA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Recombinase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">recA</name>
        <name evidence="12" type="ORF">PS3_3794</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 8">Belongs to the RecA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122834.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3794"/>
    <dbReference type="EnsemblBacteria" id="EHS84601">
        <property type="protein sequence ID" value="EHS84601"/>
        <property type="gene ID" value="PS3_3794"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1878"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00983">
        <property type="entry name" value="recA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00268">
        <property type="entry name" value="RecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013765">
        <property type="entry name" value="DNA_recomb/repair_RecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020584">
        <property type="entry name" value="DNA_recomb/repair_RecA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023400">
        <property type="entry name" value="RecA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45900">
        <property type="entry name" value="PTHR45900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00154">
        <property type="entry name" value="RecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00142">
        <property type="entry name" value="RECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54752">
        <property type="entry name" value="SSF54752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02012">
        <property type="entry name" value="tigrfam_recA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00321">
        <property type="entry name" value="RECA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="RECA_2" evidence="10">
        <location>
            <begin position="35"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="RECA_3" evidence="11">
        <location>
            <begin position="199"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="336"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="65"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS84601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="354" mass="38123" checksum="457289BBE1423A21" modified="2012-04-18" version="1">MADQREAALENALRKIEKNFGKGSIMRMGDAADTKIATVSSGSLAIDEALGVGGYPRGRIIEMYGPESSGKTTVALHAVAEVQRRGGTAAYIDAENALDPQYASALGVNIGDLLLSQPDTGEQGLEIADALIASGAVDIVVVDSVAALVPRAEIEGEMGDTHVGLQARLMSQALRKLSGEINKTKTIAIFINQIREKVGVMFGNPETTPGGRALKFYSTIRLEIRRGEQLKNGTEVIGNRAKIKVVKNKVAPPFKRAEVDIMYGEGISKTGELLDMAVEKDLVNKSGAWYSYKNDRIGQGRENAKVWLAEHPEAMNELMNEVRVAYGMEADETVATPETGEVKQETLEEASSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLB2</accession>
    <name>H4GLB2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Folylpolyglutamate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_19761</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122844.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19761"/>
    <dbReference type="EnsemblBacteria" id="EHS84611">
        <property type="protein sequence ID" value="EHS84611"/>
        <property type="gene ID" value="PS3_19761"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1890"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="PTHR11136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001563">
        <property type="entry name" value="Folylpolyglu_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01012">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="7">
        <location>
            <begin position="48"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="6">
        <location>
            <begin position="300"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR001563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="439" mass="48596" checksum="C976E288BFB5BE20" modified="2012-04-18" version="1">MTEIKTYDDALKFIHGRFKARKIPTLARMQELLKRLDNPQDGQSYIHVTGTNGKGSTVAMIRAMLMASGYQVGTFTSPFITRFNERIALNGQPISDQDLVTYTRQVATVVDQMDAEGQTPTEFEINTAIMFLYFADRNPDVVILEVGIGGLYDSTNVIEKPDIAVIVTVGYDHMQFLGDSLTEIATIKAGIIKKNSPVVVGQLPTEAKQVIGSTAKKKQSPLFSLEQEFHAQAKNSHQLAQKIEYQGLSLAKTVFQLGLAGDYQIQNAGVAITTVQLWLQKHQGYLDPSNIAEGLLNACWPGRMEIVNREPLMILDGAHNLPGIQALVQTIEHELADREVYLLVAILADKQYELMLGELASLRNVHLVLTQFAPPGPKRPTADLAGLKDVIRTRYPMQVFPEWQLGLAAISQQVSAEDVILITGSLYFISEVRNFLLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHS1</accession>
    <name>H4GHS1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">50S ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rplK</name>
        <name evidence="13" type="ORF">PS3_5738</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="PTM">
        <text evidence="7 9">One or more lysine residues are methylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000004">
        <property type="protein sequence ID" value="EHS87551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121605.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000004.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5738"/>
    <dbReference type="EnsemblBacteria" id="EHS87551">
        <property type="protein sequence ID" value="EHS87551"/>
        <property type="gene ID" value="PS3_5738"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.39"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_L11/L12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_L11/L12_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006519">
        <property type="entry name" value="Ribosomal_L11_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_L11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_L11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_L11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_L11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="PTHR11661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="SSF46906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="SSF54747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01632">
        <property type="entry name" value="L11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 9" id="KW-0488">Methylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003592382" description="50S ribosomal protein L11" evidence="10">
        <location>
            <begin position="18"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L11_N" evidence="12">
        <location>
            <begin position="9"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L11" evidence="11">
        <location>
            <begin position="71"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS87551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="141" mass="14853" checksum="FAB4570D8E6FB229" modified="2012-04-18" version="1">MAKKVANIVKLQIPAGAASPAPPVGPALGQAGINIMGFTKDFNARTADQKGMLIPVVITVYEDRSFDFITKTPPAAVLLKKAAGVEHGSGEPNTNKVATVTKDQVKEIAETKMQDLNAADVEAAMRMIEGTARSMGFVVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHS4</accession>
    <name>H4GHS4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Aldose 1-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_3767</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121608.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3767"/>
    <dbReference type="EnsemblBacteria" id="EHS87480">
        <property type="protein sequence ID" value="EHS87480"/>
        <property type="gene ID" value="PS3_3767"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.42"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09024">
        <property type="entry name" value="Aldose_epim_lacX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037481">
        <property type="entry name" value="LacX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="292" mass="33475" checksum="05874658638DA804" modified="2012-04-18" version="1">MITIENNRLSVSINELGAQLHSIKRLPDQTEYLWQGSPDSWERQAPVLFPFVGKLKDDQYTYQGQTYHQGQHGFARDMVFAVTKQSDHAVTFQLKDNEQTRQVYPFAFELNISYRLVDDQLLVNYLVVNPSQDQTLIYALGAHPGFRTPLSDTSNFEDVEISTEPAEELDRFKLVGPYNDRQHPFKMDMHQPIMANHDLFDEDAIIFETHGEPFAVNLTDTKTQHGVKVSVIDTPYVGVWSAYPKLGNFICIEPWWGVADAVDADGRLEDKDAMHRLIPQDSDTYSFSIKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHU0</accession>
    <name>H4GHU0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7 9">GTPase Der</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">GTP-binding protein EngA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">der</name>
        <name evidence="11" type="ORF">PS3_8977</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">GTPase that plays an essential role in the late steps of ribosome biogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8 9">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngA (Der) GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121624.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8977"/>
    <dbReference type="EnsemblBacteria" id="EHS87496">
        <property type="protein sequence ID" value="EHS87496"/>
        <property type="gene ID" value="PS3_8977"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.59"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00195">
        <property type="entry name" value="GTPase_Der"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031166">
        <property type="entry name" value="G_ENGA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016484">
        <property type="entry name" value="GTP-bd_EngA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032859">
        <property type="entry name" value="KH_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14714">
        <property type="entry name" value="KH_dom-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006485">
        <property type="entry name" value="GTP-binding_EngA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03594">
        <property type="entry name" value="GTPase_EngA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51712">
        <property type="entry name" value="G_ENGA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="EngA-type G" evidence="10">
        <location>
            <begin position="4"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="EngA-type G" evidence="10">
        <location>
            <begin position="175"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="10"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="57"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="119"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="181"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="229"/>
            <end position="233"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="294"/>
            <end position="297"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="437" mass="49286" checksum="C8756E667D0E0EF5" modified="2012-04-18" version="1">MANPIVAIVGRPNVGKSTLFNRIAGERISIVEDTPGVTRDRIYAHGDWLGKEFSMIDTGGIEISDQPLTTQIREQAEVAIDEADVIVMVVNVENGVTDADEQVAKILYRSHKPVVLAVNKVDNPERRSQIFDFYSLGLGEPYPVSSVHGVGIGDLLDEVIKHFPERSNTQDDQRIRFSFIGRPNVGKSSIVNAMLGEDRVIVSDIAGTTRDSIDTNFKTPDGHEFTMVDTAGIKKKGKLYENTERYALMRSMRAIDDSDVVIFVLNAEEGIRELDKHVAGYAHEAGKAIIIAVNKWDTIPDRDQRTMTDFTNLIRQEFQYLSYAQIIFVSAKTKQRLFKLPEMVLEAYEHHTRRVQSSVLNDVIMDAVAANPAPSSNGRRLRLYYATQVAIQPPTFVVFVNDPDLMHFSYERYLENRIRAAFDFTGTPIHLIKRQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHU2</accession>
    <name>H4GHU2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytidylate kinase</fullName>
            <shortName evidence="8">CK</shortName>
            <ecNumber evidence="8">2.7.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cytidine monophosphate kinase</fullName>
            <shortName evidence="8">CMP kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cmk</name>
        <name evidence="10" type="ORF">PS3_9397</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + CMP = ADP + CDP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>ATP + dCMP = ADP + dCDP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the cytidylate kinase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121626.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9397"/>
    <dbReference type="EnsemblBacteria" id="EHS87498">
        <property type="protein sequence ID" value="EHS87498"/>
        <property type="gene ID" value="PS3_9397"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.61"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036430">
        <property type="term" value="F:CMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02020">
        <property type="entry name" value="CMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00238">
        <property type="entry name" value="Cytidyl_kinase_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003136">
        <property type="entry name" value="Cytidylate_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011994">
        <property type="entry name" value="Cytidylate_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02224">
        <property type="entry name" value="Cytidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00017">
        <property type="entry name" value="cmk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidylate_kin" evidence="9">
        <location>
            <begin position="7"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="11"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="225" mass="24674" checksum="90A81C393B2FA0BE" modified="2012-04-18" version="1">MTKGFQVAIDGPASAGKSTVAKLVAQKFGYIYCDTGAMYRAVTLAALQQKIDLNDQVKVAAIAENLPLRFEAGDPQQVWLGDQNVTQLIRTNEVAQNVSTVAAIPAVRQAMVKMQREIASQNDIVMDGRDIGTTVLPQAPVKIFMVASAHERARRRYLDNQARGIATQSIEELQKAIELRDQKDSTRKVSPLVQAADAIRLDTTALTIDEVVNEISEIIKKVKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHV3</accession>
    <name>H4GHV3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase T</fullName>
            <ecNumber evidence="7">3.4.11.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Aminotripeptidase</fullName>
            <shortName evidence="7">Tripeptidase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Tripeptide aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pepT</name>
        <name evidence="11" type="ORF">PS3_4555</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Cleaves the N-terminal amino acid of tripeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Release of the N-terminal residue from a tripeptide.</text>
            <dbReference type="EC" id="3.4.11.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7 9">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase M20B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.4" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121637.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4555"/>
    <dbReference type="EnsemblBacteria" id="EHS87509">
        <property type="protein sequence ID" value="EHS87509"/>
        <property type="gene ID" value="PS3_4555"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.78"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045148">
        <property type="term" value="F:tripeptide aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00550">
        <property type="entry name" value="Aminopeptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010161">
        <property type="entry name" value="Peptidase_M20B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42994:SF1">
        <property type="entry name" value="PTHR42994:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037215">
        <property type="entry name" value="Peptidase_M20B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01882">
        <property type="entry name" value="peptidase-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="M20_dimer" evidence="10">
        <location>
            <begin position="212"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="active site" evidence="7 8">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7 8">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037215-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037215-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="413" mass="45609" checksum="F05699FCF282D1A0" modified="2012-04-18" version="1">MESRYPGLLERFLKYAKQNTRSDAESSTIPSTPRQTAFLKELQTELTSIGITDAKINPENSYLTGTIPSNLDQEVPVMGLIAHIDTADFNAENIQPQVIENYDGVSDIHLGDSPYVLSPQEFPALKNYAGETLVTTDGTTLLGADDKAGVSAIVTFAEYLFQHPEIKHGKIRLGFGPDEEIGTGADHFDVDDFGADFAYTVDGGPLGELEYETFNAAQATINFTGKNVHTGVAKDTMINALQLAIDFHNQLPTHDRAELTADREGFFHLYQLGGTVDAAQATYLIRDHDRQLFEFRKTALEAIARDMNARFDQDRVQVKISDQYYNLKDALNGHLESVEMAKQAMEALSIKPIIYPVRGGTDGSKISYMGLPTPNLFAGGENMHSRFEFVSVQTMEQVVNVLLKISDLNIADN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHW0</accession>
    <name>H4GHW0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">4-hydroxy-tetrahydrodipicolinate synthase</fullName>
            <shortName evidence="9">HTPA synthase</shortName>
            <ecNumber evidence="4 9">4.3.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dapA</name>
        <name evidence="11" type="ORF">PS3_538</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:34171"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="4.3.3.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the DapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.7" evidence="4 9"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121644.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_538"/>
    <dbReference type="EnsemblBacteria" id="EHS87516">
        <property type="protein sequence ID" value="EHS87516"/>
        <property type="gene ID" value="PS3_538"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.85"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00017"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00950">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00418">
        <property type="entry name" value="DapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005263">
        <property type="entry name" value="DapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020625">
        <property type="entry name" value="Schiff_base-form_aldolases_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="PTHR12128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00674">
        <property type="entry name" value="dapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00666">
        <property type="entry name" value="DHDPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="9">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="9">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="307" mass="33296" checksum="516D728C8E3538F6" modified="2012-04-18" version="1">MSNTNLKQADLMTAIITPFDEHGINYQSLDRLTEHLLSLGNKGFVVGGTTGETPTLSHDEKLALYEHFAKLINGRVPVIAGTGSNNTQETIEFTNEVAQIEGIDYALVVVPPYNKPNQRGMIAHFTAVADQVDLPIIIYNIPGRTGVKMAQETVVTLSQHPNIAGVKQCASMEEFEYIVENSADDFAVFSGEDNQAFIAKALGGAGVISVAAHLYAKQMRAMYDALDNGEVATAAKLQRWLTPRMQACFMYPSPSPIKAVMNAQGFEVGPCRLPIVSLNDEEKQTLAQALGLGQDAFSHELPLDLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHY3</accession>
    <name>H4GHY3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP diphosphokinase</fullName>
            <ecNumber evidence="3">2.7.6.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_7730</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate</text>
            <dbReference type="Rhea" id="RHEA:22088"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:142410"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the relA/spoT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.5" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121667.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7730"/>
    <dbReference type="EnsemblBacteria" id="EHS87539">
        <property type="protein sequence ID" value="EHS87539"/>
        <property type="gene ID" value="PS3_7730"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.110"/>
    <dbReference type="UniPathway" id="UPA00908">
        <property type="reaction ID" value="UER00884"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008728">
        <property type="term" value="F:GTP diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015970">
        <property type="term" value="P:guanosine tetraphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05399">
        <property type="entry name" value="NT_Rel-Spo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01668">
        <property type="entry name" value="TGS_RSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004811">
        <property type="entry name" value="RelA/Spo_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045600">
        <property type="entry name" value="RelA/SpoT_AH_RIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007685">
        <property type="entry name" value="RelA_SpoT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033655">
        <property type="entry name" value="TGS_RelA/SpoT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13291">
        <property type="entry name" value="ACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19296">
        <property type="entry name" value="DUF5913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04607">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00954">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00691">
        <property type="entry name" value="spoT_relA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HD" evidence="8">
        <location>
            <begin position="50"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="9">
        <location>
            <begin position="395"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="670"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="574"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="579"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="744" mass="84681" checksum="D661D08CBF009BFF" modified="2012-04-18" version="1">MSKMKNLTHEDVRNICASYMNDEHVQLVEKAYQFAMICHNGQKRKSGELYIVHPIQVAGILAKLHMDPETVCAGYMHDVVEDTGATLDDIEVLFGRTIALIIDGDTKISKIHYKSNREQMAETHRKLLLAMSQDIRVMIVKLADRLHNMRTLDSLREEKQKRISRETLEIYAPIADRLGISTMKWELEDLSLRYIDPEQYYTIVHLMHSRREQRVDYIDHAIEVVQKAVDELNLQGNVEIYGRPKHIYSIYRKMVNKHKQFSEIYDLLAIRVVVDTVPDCYAVLGAIHTNWKPMPGRFKDYIAMPKVNGYQSLHTTVIGPGGQPLEIQIRTHEMHEVAEFGVAAHWAYKEGKTDGIKQNLDSQKLNIVKEIIEMRNESQDTDDFMSGVQSDIFADKVYAFTPKGDVIEMPYGAGPLDMAYQIHTEVGNKTTGAKVNGRIVPLDYEIKNGDIVDIITSNSSHPSHDWLDLVSTRRAKNKIRQYFRMHDRQKNIEDGRHLIEQQVREAGFDPSKIVTDELATRVAERMHYNTADELFAAVGFGDISPMGARNRFTEDIRREEKKRAAEAKAQAILENNETLEPAEEEKKKKLPKSSGDGIIVEGVDNLLTRISQCCKPVPGDKILGYITMGRGVSVHREDCPNIKHAEQTGQRIVTVYWENPAGDQTTYDTDIMVVGDNQSGLVNEVLRAINNNTRYLNSINGKVDSNNVATINATVGVRSTKQLEIIMNSLRNLANVYEVRRVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHY6</accession>
    <name>H4GHY6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_13734</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13734"/>
    <dbReference type="EnsemblBacteria" id="EHS87542">
        <property type="protein sequence ID" value="EHS87542"/>
        <property type="gene ID" value="PS3_13734"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.114"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="101" mass="12440" checksum="F4BB4F2491830F2F" modified="2012-04-18" version="1">MITVLNRTKQFMRDNDINYKKEYIRPMIGPEHVYIFRFGKRKLNNRVIIRYSHTWTGRLKINEIDVRLHRQHHPRIFKSESELIDYLQLHLDKNHEINADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHY7</accession>
    <name>H4GHY7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fructokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_6628</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000006">
        <property type="protein sequence ID" value="EHS87466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121671.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000006.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6628"/>
    <dbReference type="EnsemblBacteria" id="EHS87466">
        <property type="protein sequence ID" value="EHS87466"/>
        <property type="gene ID" value="PS3_6628"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.115"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01125">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="290" mass="31959" checksum="0EE11B52F056BB5F" modified="2012-04-18" version="1">MLLGSIDSGGRRLTCAVADEHQQIIKKALYLTTDPAATFKHIVDFFSPYQEELAALGVASFGPIDIDPQSPTFGYITTTPKVGWKNVNFVGELKKHFNLPIYWTTGVNASVYGEYLNLKDQLHHGSLVYFTLGTGVGAGAIQNGNFIGGVGAPEMGHVFVKRHPDDRTFTGICPYHGDCLEGLVGGPTFQARLGIRGEDVDDTDHVWDILAYYVAQASIQATTILRPEKIIFGGSVARPQLIKKVREQFERMFNHYMEVGEIEDYIIHPSVPRNESATRGNFALAAELLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI02</accession>
    <name>H4GI02_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent zinc metalloprotease FtsH</fullName>
            <ecNumber evidence="5">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ftsH</name>
        <name evidence="9" type="ORF">PS3_5404</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the central section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000007">
        <property type="protein sequence ID" value="EHS87453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5404"/>
    <dbReference type="MEROPS" id="M41.009"/>
    <dbReference type="EnsemblBacteria" id="EHS87453">
        <property type="protein sequence ID" value="EHS87453"/>
        <property type="gene ID" value="PS3_5404"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.132"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="SSF140990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="8">
        <location>
            <begin position="200"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="609"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="613"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="655"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="431"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="208"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="506"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="684" mass="74806" checksum="931C5BA80B87A999" modified="2012-04-18" version="1">MAGVYFFFGGSSSTGEQKEVSQSTFIKELRKGEVKSVQLQPSNGVYRVTGEYKKAKTTSSSSTTGLSLGASSSTTTKQFETSVLENDATVSEIQRYANQTNTKISTKGEESNSIWSNLLVSLLPLVIIIIFFYMMMGQAGSGGGRGIMNVGKSKAKPSDPKKNKVRFGDVAGEEEEKQELVEIVEFLKNPKKFSRLGARMPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFDQAKKSAPSIIFIDEIDAVGRRRGNGQGGGHDEREQTLNQLLVEMDGFQGDEGVIVMAATNRSDVLDPALLRPGRFDRKVLVGRPDVKGREAILRVHAKNKPLAADVDLKEIAKQTPGFVGADLENLLNEAALLAARRNKDQIDRLDMDEAEDRVIAGPAKRDRVISKQERETVAYHEAGHTIVGLVLNDARVVHKVTIVPRGRAGGYAIMLPREDQMLMSKKNAEEQIAGLMGGRAAEEIIFKSQSSGASNDFEQATQIARSMVTQYGMSDKIGPVELQSSGAVFTGQGFDQSSYSQETAATVDAEIRRILMEGHEQALHIIETHREQHKLIAEALLKYETLDEKQILSLYQTGKMPEDEAESQNEEQRAQTFEEAKKALERRENERAQEYSEDELDSDTNQASDSSSTATSEDLTLTSEQTNFDHSEDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI06</accession>
    <name>H4GI06_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cystathionine beta-synthase (CBS) domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_13226</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000007">
        <property type="protein sequence ID" value="EHS87457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121690.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000007.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13226"/>
    <dbReference type="EnsemblBacteria" id="EHS87457">
        <property type="protein sequence ID" value="EHS87457"/>
        <property type="gene ID" value="PS3_13226"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.139"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017036">
        <property type="entry name" value="UCP035040_CBS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF035040">
        <property type="entry name" value="UCP035040_CBS_Lmo0553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="7"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="211" mass="24083" checksum="B00B522328D31B84" modified="2012-04-18" version="1">MILEDLIQPLNKLTTVKEDATLAEALDILEDTGYRCVPILDESEKIFRGNIYKMHLYRHKSRGGDMNLPVTALLKNATKFISIDDDFFTVFFTLRDLPYIAVLDHQKHFYGILTHNHLINMLSAAWNVNTGSYVLTIQANEERGFLVSVAKEITRYCQIANVMTLTPTGSKQLIVTLPEDVTPELFAKITNRLAKKHIQVLHIEDLRQAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI22</accession>
    <name>H4GI22_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phosphate ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_12659</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000011">
        <property type="protein sequence ID" value="EHS87432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121706.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000011.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12659"/>
    <dbReference type="EnsemblBacteria" id="EHS87432">
        <property type="protein sequence ID" value="EHS87432"/>
        <property type="gene ID" value="PS3_12659"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.161"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015415">
        <property type="term" value="F:ATPase-coupled phosphate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="Phosp_transpt1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="PTHR43423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00972">
        <property type="entry name" value="3a0107s01c2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="6"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="250" mass="28002" checksum="D306F09B3982516B" modified="2012-04-18" version="1">MQESALELQKVCVAYGDKPIVHQVSMEFPKHQISALIGASGSGKSTLLRSINRMNDGLATVTGQIIFDGIDINQPEINVYEVRRSIGMVFQQPTPFPKSIYENVIYGLRLDGVKDRQLLDERVETSLKQAALWEEVKDQLHQSALSLSGGQQQRLCIARTLAVRPEIILMDEPTSALDPISTSQIENTLHDLKDHFTIIIVTHNMQQASRASDWTAFLDQGNLIEFNPTEQMFMNPQQTKTADYLSGKFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI28</accession>
    <name>H4GI28_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Nuclease SbcCD subunit D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">sbcD</name>
        <name evidence="11" type="ORF">PS3_16915</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'-&gt;5' double strand exonuclease that can open hairpins. It also has a 5' single-strand endonuclease activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 8">Heterodimer of SbcC and SbcD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the SbcD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000011">
        <property type="protein sequence ID" value="EHS87438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121712.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000011.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16915"/>
    <dbReference type="EnsemblBacteria" id="EHS87438">
        <property type="protein sequence ID" value="EHS87438"/>
        <property type="gene ID" value="PS3_16915"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.167"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004593">
        <property type="entry name" value="SbcD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026843">
        <property type="entry name" value="SbcD_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12320">
        <property type="entry name" value="SbcD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00619">
        <property type="entry name" value="sbcd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 8 11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="9">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="SbcD_C" evidence="10">
        <location>
            <begin position="259"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="370" mass="41613" checksum="9667104720031D9D" modified="2012-04-18" version="1">MRFLHTADWHVGKSLHGYDLLKEQKHIFQQIEAIALEQKVDAIVIAGDLYDRGAPSEEAVSELNAELQRLNLQDRLPILAISGNHDSAARLATGDAWYSATQFYLHTQLSQAFEPIVIGDTQFFLLPYFELQAVRNYFGDDEIHSVKVAMERIVAQMQDHFAPDKHQVLVAHFFAAGSSHTDSETKVEVGGLNAVPVDLLTAFDYVTLGHLHNPNALKNPVIKYSGSPLKMSASEAELQKGVRIVDSATGESTWVPLKPAHDVRIIKGSFDDLMQKTDEGDRDDFVVIQLTDQEMIPDVMSRLRQVYPRIIALQRVNGPVQMKIDTTIDPALDPMQLVAKFYDFATDGQRLDHEQKEWLTQSLQRVQKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI53</accession>
    <name>H4GI53_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">rRNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_19577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19577"/>
    <dbReference type="EnsemblBacteria" id="EHS87392">
        <property type="protein sequence ID" value="EHS87392"/>
        <property type="gene ID" value="PS3_19577"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.200"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SpoU_sub_bind" evidence="1">
        <location>
            <begin position="32"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="254" mass="27535" checksum="4FE8EB71F89F1056" modified="2012-04-18" version="1">MVEIITSTQNQRVKQWKKLQTKKGRQQSQSYLLDGWHLVQEALQSQVQITALIGTKAELALHNDIVARVNQVYQVTGGIMQEITATVTPQGIAAVVGSPLLQLKTIPRSGKWLLLDRVQDPGNVGTMVRTADAAGFNGVVAGKGTVDFFSPKVMRSMQGSQFHIRLATAELSDLIPAMQANGMPVYGTELNPQAISYRELPQSDQVAFVMGNEGQGMADNLLQLTTKNVYIPMPGQAESLNVAVSAGILMFSVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI89</accession>
    <name>H4GI89_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Multidrug export protein MepA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_13400</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MepA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121773.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13400"/>
    <dbReference type="EnsemblBacteria" id="EHS87310">
        <property type="protein sequence ID" value="EHS87310"/>
        <property type="gene ID" value="PS3_13400"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.249"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13143">
        <property type="entry name" value="MATE_MepA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045070">
        <property type="entry name" value="MATE_MepA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="385"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="450" mass="49149" checksum="ADF8DC7DB5AAB9EE" modified="2012-04-18" version="1">MDKLFEHTSIPKAYFTLAMPVVLSMVVTLVYNMVDTFFVSQTQNPNLVAGVSQSAPIFTFLIALGDIFGLGGSSVISRLFGEKQDQLGRNVSGYAFYGSILCGIIVTIIMLVFKTPILHLLGATSATWQYANEYYTVLVSGATFIVFGLAPTNILRTEGLALESMKASMIGTILNIILNPIFIFPLGLGAAGSATATVISQIISDGFLIYYTHTKSTRLTTSIKETKISRHLQWELFAIGIPASVTNIMSTFAIALTNHYLIPYGADSVATMGIALKISTIINMVFVGFAFGAQPLIGYTYGAKDAKRFNQIMKFDLQVVCGFSIIMTVLMFILAPTLMKGFLHDPRVISEGAGMIRWLVLSSTFAGIMLVFTTMFQSMGKAFPAFLLSVSRQGLIFFIVIVITSQLFGYTGVIVAQPIADVLTAGLGILLFLIYRPRFKSKTRRERNVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIA0</accession>
    <name>H4GIA0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_20645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121784.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20645"/>
    <dbReference type="EnsemblBacteria" id="EHS87321">
        <property type="protein sequence ID" value="EHS87321"/>
        <property type="gene ID" value="PS3_20645"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.265"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017195">
        <property type="entry name" value="ABC_thiamin-permease_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09819">
        <property type="entry name" value="ABC_cobalt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037394">
        <property type="entry name" value="ABC_thiamine-permease_YkoE_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="187" mass="20178" checksum="64CE12C2E7C30784" modified="2012-04-18" version="1">MQHKGFSLKDIILLALIGIVFGVIFVGAGYLYNIIMLALTPVGFGPAAEDITIGLWCMAGPLAGFLLRRPGAAFLGEVIGAVVEMLIGDQWGAANLLSGAVQGLGSELGFAFTGYKIYNWLTAILTTLTTCAVTFVWDWFKSGYDAFKPSTVITLLIIRIICMFIIDGVLVKLISNLLERSQVLKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIA5</accession>
    <name>H4GIA5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Argininosuccinate lyase</fullName>
            <shortName evidence="7">ASAL</shortName>
            <ecNumber evidence="3 7">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">argH</name>
        <name evidence="10" type="ORF">PS3_7752</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="3 7"/>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121789.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7752"/>
    <dbReference type="EnsemblBacteria" id="EHS87326">
        <property type="protein sequence ID" value="EHS87326"/>
        <property type="gene ID" value="PS3_7752"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.270"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="PTHR43814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lyase_1" evidence="8">
        <location>
            <begin position="9"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="ASL_C2" evidence="9">
        <location>
            <begin position="366"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="459" mass="51393" checksum="75BFA30D850264B6" modified="2012-04-18" version="1">MSIQTAWGGRFQSAGAKWVDEFGASIDFDQLMAHEDIEGSLAHVQMLGHTGILTSPEVQQIKTGLEDLQADLDAGKLSFSVEREDIHMNLEALLTEKIGPVAGKLHTARSRNDQVATDFHLYLKHRLPLIIETIKELQRVLVQRAEENVETIMPGYTHLQHAQPISYGHYLMAYYAMLKRDVERFEFNQHHTDIMPLGSAALAGTTFPIDREYTAQLLDFGQIYTNSLDAVSDRDFALEFLSNAAILMMHLSRLSEELISWCSYEFNYLEMADEFSTGSSIMPQKKNPDMAELTRGKTGRVYGHLMGLLSTMKGLPLAYNKDMQEDKEGVFDTVKTIIPCLKVMTGMIKTLSVHPDKMAAATKNDFSNATELADYLATKGIPFRQAHAIVGNLVYEGLQSGKNLQDISLADYQKIDPRIEEDVYQVLDPKVAVQRRTSLGGTGFDQVKAQIKQAHAELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIC5</accession>
    <name>H4GIC5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_7734</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121809.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7734"/>
    <dbReference type="EnsemblBacteria" id="EHS87346">
        <property type="protein sequence ID" value="EHS87346"/>
        <property type="gene ID" value="PS3_7734"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.304"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="4"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="HTH cro/C1-type" evidence="5">
        <location>
            <begin position="4"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="335" mass="38121" checksum="07D7CC244D12BF6B" modified="2012-04-18" version="1">MARLTIKDIAELVGVSTATISNYLNGNYNKMSDRTRQQIAEVIGQANYHPSSIARGLAKNENRTIGVSIADITNPFTSQVVSGISEVCNKHDYKVIFTNSDNDPKHEIKNLVRLRSEQVSGYIIDPVNPNGTLFKTFSNENTIMVDRQANEIKVDTIVTDNESSVKEMVSLMKNSGIDEMYFVSWPLGGVSTRIQRFKGFLEAMNYPNNDSHLIQVPHHGAPEEYDHFKNTIQEIMANRGKKRIGFFSMNGRVFLRLIDAMQTLSYHYPDDYALATYEEFSWIRVFRPRISCIRQDSRLIGITAASNLIDKLENKTEQSPRLITMPTEIRLHESF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIE6</accession>
    <name>H4GIE6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Alpha-galactosidase</fullName>
            <ecNumber evidence="3 6">3.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PS3_10539</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.</text>
            <dbReference type="EC" id="3.2.1.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.22" evidence="3 6"/>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121830.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10539"/>
    <dbReference type="EnsemblBacteria" id="EHS87367">
        <property type="protein sequence ID" value="EHS87367"/>
        <property type="gene ID" value="PS3_10539"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.342"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052692">
        <property type="term" value="F:raffinose alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14791">
        <property type="entry name" value="GH36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038417">
        <property type="entry name" value="Alpga-gal_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000111">
        <property type="entry name" value="Glyco_hydro_27/36_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002252">
        <property type="entry name" value="Glyco_hydro_36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031705">
        <property type="entry name" value="Glyco_hydro_36_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031704">
        <property type="entry name" value="Glyco_hydro_36_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16874">
        <property type="entry name" value="Glyco_hydro_36C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16875">
        <property type="entry name" value="Glyco_hydro_36N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005536">
        <property type="entry name" value="Agal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00743">
        <property type="entry name" value="GLHYDRLASE36"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00512">
        <property type="entry name" value="ALPHA_GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_36N" evidence="10">
        <location>
            <begin position="31"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_36C" evidence="9">
        <location>
            <begin position="652"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="366"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="476"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="478"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="548"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="526"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="548"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR005536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005536-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005536-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="739" mass="84853" checksum="BA23F6121EB8C915" modified="2012-04-18" version="1">MSINVNEEHQLFHLQTANTSYIFHVMINGDAGQLYYGTKIPVKADYLNLDTHEEHDCTNTLTDDQTDFQLELLKQEYASFGKGDFRYPAFQLTNPDGSRISEFKYHHYELTNGKERLAGQPSAFASDEDDSQTLKLHFLDDVNQVELVLNYTVFENEDVIVRSSTFINHGAPVELNRALSMQLDLPDRQYDLVQFDGSWARERHLHRSPLRYGTQSNSSLRLHSGHQSNPFFMLARPHTDNNQGGAFGFNLIYSGNFLDSVEVDQFDTTRVLVGINPEEFGWHLGTDESFQTPEAVLTYTDNGFNQLSQQLGAFYNHHLLNQNFVNQDRPILINNWEATFMDFTEDKLMPIVEKAKELGIEMFVLDDGWFGHRDNDLSSLGDWFVDQKKFINGIGGFSYRVHNLGMKFGLWFEPEMISVDSELFQQHPEWRIGAPKRQLTPARHQFVLDMSRPEVVDYLYEKMSAIIKDTQLDYIKWDMNRSITEAFSADLPADRQLEFGHRYILGVYDLYDRLTKAFPKVLFESCASGGGRFDLGMMYYAPQAWASDDTDAVERLAIQDGTSYGYTQNMWGAHVSAVPNDQVGRLTSIDLRAKVAYFGDFGYELDITKLSPEEQATIRDQVAFYKQYRHLFQFGKFYRLESPDSDAQNVFSWEVVNDDQTEAIGARFQVLNGANPAYIRFYFAGLDPEKQYTVNDSDEVFSGQELMSAGYFVPRVMPRTPEKDPADFKGSLFIAKAIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GII9</accession>
    <name>H4GII9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoesterase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_10195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000020">
        <property type="protein sequence ID" value="EHS87268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121873.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000020.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10195"/>
    <dbReference type="EnsemblBacteria" id="EHS87268">
        <property type="protein sequence ID" value="EHS87268"/>
        <property type="gene ID" value="PS3_10195"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.394"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHH" evidence="1">
        <location>
            <begin position="16"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="232"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="319" mass="35372" checksum="302F196ECA0B1A4D" modified="2012-04-18" version="1">MDNLTAILTAIKKYNRIIIHRHQRPDPDAIGSQTGLAEILRSSFPHKEIYMVGKDIPSFDWIGSMDEIDGDEYHNALVIVIDTANTPRIDDRRYNNGDFLIKIDHHPNDEPYGDLMWVQPDASSCSELVYDFYNQFADQLTLPESAAHALYAGIVGDTGRFLYPATTSHTLAVAGALMEHGAQAAKISQRENEITFEMARLSAYVYNHLSINAHGVGQVKLSNEVLKDYGLTEATTSAVVPLPGQIHTVLAWAIFVQQEDGEYRARLRSKGPAINEVAKQHRGGGHPLASGAKAADDAECEQIIADLDQVCRQYLEKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIK0</accession>
    <name>H4GIK0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphatidylglycerol--prolipoprotein diacylglyceryl transferase</fullName>
            <ecNumber evidence="2">2.5.1.145</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lgt</name>
        <name evidence="3" type="ORF">PS3_5731</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the transfer of the diacylglyceryl group from phosphatidylglycerol to the sulfhydryl group of the N-terminal cysteine of a prolipoprotein, the first step in the formation of mature lipoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + L-cysteinyl-[prolipoprotein] = H(+) + S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[prolipoprotein] + sn-glycerol 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:56712"/>
            <dbReference type="Rhea" id="RHEA-COMP:14679"/>
            <dbReference type="Rhea" id="RHEA-COMP:14680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
            <dbReference type="ChEBI" id="CHEBI:140658"/>
            <dbReference type="EC" id="2.5.1.145"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; lipoprotein biosynthesis (diacylglyceryl transfer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Lgt family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.145" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121884.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5731"/>
    <dbReference type="EnsemblBacteria" id="EHS87195">
        <property type="protein sequence ID" value="EHS87195"/>
        <property type="gene ID" value="PS3_5731"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.409"/>
    <dbReference type="UniPathway" id="UPA00664"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008961">
        <property type="term" value="F:phosphatidylglycerol-prolipoprotein diacylglyceryl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042158">
        <property type="term" value="P:lipoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01147">
        <property type="entry name" value="Lgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001640">
        <property type="entry name" value="Lgt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30589">
        <property type="entry name" value="PTHR30589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01790">
        <property type="entry name" value="LGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00544">
        <property type="entry name" value="lgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01311">
        <property type="entry name" value="LGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol)</name>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="279" mass="32129" checksum="D549D50557085A13" modified="2012-04-18" version="1">MKIELINGAINPIALQWGPFRVHWYGIIIASAVILALWLSIREGRRLGIPEDDFYDALLWSLPIAVICARTYYVVFQWSYYSHNLSEIIAIWDGGIAIYGALIGGLITILCFCHYRHLNAWLFLDVLAPSVILAQAIGRWGNFMNQEAYGLVTTKAHLMALHLPTWIINQMYIGGAYRVPTYLYESVGDLLGFVILLIIRHRHHWLKQGEVLCGYLGWYALVRMIVEGMRTDSLMLGQLRVSQWLSVVILIAVVIIVIYRRRHFDLPWYSDLLIKEKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIL0</accession>
    <name>H4GIL0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Biotin carboxylase</fullName>
            <ecNumber evidence="3 11">6.3.4.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Acetyl-coenzyme A carboxylase biotin carboxylase subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PS3_9415</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 11">This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + hydrogencarbonate + N(6)-biotinyl-L-lysyl-[protein] = ADP + H(+) + N(6)-carboxybiotinyl-L-lysyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13501"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.14"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="11">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Acetyl-CoA carboxylase is a heterohexamer of biotin carboxyl carrier protein, biotin carboxylase and the two subunits of carboxyl transferase in a 2:2 complex.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.14" evidence="3 11"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121894.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9415"/>
    <dbReference type="EnsemblBacteria" id="EHS87205">
        <property type="protein sequence ID" value="EHS87205"/>
        <property type="gene ID" value="PS3_9415"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.421"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004549">
        <property type="entry name" value="Acetyl_CoA_COase_biotin_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00514">
        <property type="entry name" value="accC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0092">Biotin</keyword>
    <keyword evidence="11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="13">
        <location>
            <begin position="1"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="12">
        <location>
            <begin position="122"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS87205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="460" mass="51457" checksum="B73EEF3A3E542A4E" modified="2012-04-18" version="1">MFSKVLVANRGEIAVRIIRSLRELGIKSVAIYSTADRDSLHVQLADQAVCVGTQRPQDSYLNMNNILAAAIGTGAEAIHPGFGFLSENAEFAEMCEDLGIKFIGPRPETIDQMGNKEHAREQMIKGHVPVIPGSDGYVQTAEEAKQVADQIGYPVLLKAASGGGGKGIRRIESAQELARSFNEAQSEARVSFNDDRMYLEKIMENVKHIEVQVFRDDFNNAVYFPERDCSLQRNKQKVIEESPCPLITEEQRQELGEIALRAIETIDYRNTGTLEFLMDQDHHFYFMEMNTRIQVEHTVTEMVTGIDIVKAQVTVAAGEPLPFSQADIQLNGHAIECRINAEDPQKNFMPSTGEVSYLYLPVGNLGMRIDTALYPGSVVTPFYDSMIAKVIALGNDRTEAIEKLKRLINEMVIMGITTNQKFHLAILNDPEFIAGTFTTNYLEQEFLPRWKGEVRSETVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIL6</accession>
    <name>H4GIL6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acyl carrier protein</fullName>
            <shortName evidence="1">ACP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">acpP</name>
        <name evidence="4" type="ORF">PS3_12670</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by AcpS. This modification is essential for activity because fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121900.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12670"/>
    <dbReference type="EnsemblBacteria" id="EHS87211">
        <property type="protein sequence ID" value="EHS87211"/>
        <property type="gene ID" value="PS3_12670"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.427"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="Acyl_carrier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="PTHR20863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1 2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="3">
        <location>
            <begin position="4"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="1 2">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="81" mass="9309" checksum="B334322B39420F2E" modified="2012-04-18" version="1">MTKEEIFASVKEVVVEELDLDEDQVTLDAKIKEDLDADSLDVFEIMNELEDKFDIQLDVDENVQTIGDVVDFVKKQMDEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIL8</accession>
    <name>H4GIL8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13433</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121902.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13433"/>
    <dbReference type="EnsemblBacteria" id="EHS87213">
        <property type="protein sequence ID" value="EHS87213"/>
        <property type="gene ID" value="PS3_13433"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.429"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="6"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="148" mass="17152" checksum="4D12ABE96EE7DF3E" modified="2012-04-18" version="1">MKNDDYREIAQALTRVNTAITWIEEQELRKSRFNDLTLKELHAIDAITMYDQPTASVLAERLHVAPGTATATIDRLCRKGYAQRLSDQHDRRVIRITLTKKGRLIYRTQRAFHDRLANSFLKDVDDDQLGVIKKSLANLEDFLNVNLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIN3</accession>
    <name>H4GIN3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutaredoxin-like protein NrdH</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_8297</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Electron transport system for the ribonucleotide reductase system NrdEF.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121917.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8297"/>
    <dbReference type="EnsemblBacteria" id="EHS87228">
        <property type="protein sequence ID" value="EHS87228"/>
        <property type="gene ID" value="PS3_8297"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.447"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097573">
        <property type="term" value="F:glutathione oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011909">
        <property type="entry name" value="GlrX_NrdH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02194">
        <property type="entry name" value="GlrX_NrdH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="4">
        <location>
            <begin position="2"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="73" mass="8245" checksum="492611E18AAF754E" modified="2012-04-18" version="1">MVTVFSKNNCIQCKMTKKFLEQHNVAFVEHNIDEQPEFVDELKANGFMATPVVQLPDGNAFSGFRPDMLRGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIR3</accession>
    <name>H4GIR3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Multidrug transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_3030</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the major facilitator superfamily. TCR/Tet family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121947.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3030"/>
    <dbReference type="EnsemblBacteria" id="EHS87258">
        <property type="protein sequence ID" value="EHS87258"/>
        <property type="gene ID" value="PS3_3030"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.483"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="215"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="286"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="369"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="9">
        <location>
            <begin position="8"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="395" mass="44315" checksum="B7B3A6D819FFDB3D" modified="2012-04-18" version="1">MTQKTKRTIYIMLISQFLIFLGMSLIFPVEPYIRKEYHLTAFDMGMMSALFALVQFAASPIAGRLSDRWGRKPMMVWGLVMFTVGEFIFAMGQNLFWFDFSRMIDGLSAAMFTPASMALAADITTEQQRAKVIGWLSAAFSGGLILGPGLGGILANISYKFPFWIAGILGVISILVTVIWLPKDDEHDLKAHAQMAEEYRSASSWEQLKQIMSPALMMLCVMILIAAFGLAGFESIYSLYVNQVHGFDLNQIATVLTLNGILSLILQVFLFEWLVDKLKEVGLIRLTYFVAIIGTVMVIYLHHYWLVVFATLLVFEGFDLVRPAITTLMTKLGRDNQGLLNGVNTSLTSVGNVFGPLLSGWLLDVNSLYPYWLVNVFLVIAFLITFSLARQMKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIU5</accession>
    <name>H4GIU5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">30S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpsH</name>
        <name evidence="7" type="ORF">PS3_5717</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 30S ribosomal subunit. Contacts proteins S5 and S12.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121978.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5717"/>
    <dbReference type="EnsemblBacteria" id="EHS87175">
        <property type="protein sequence ID" value="EHS87175"/>
        <property type="gene ID" value="PS3_5717"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.526"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_B">
        <property type="entry name" value="Ribosomal_S8_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_S8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_S8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="PTHR11758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="SSF56047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="132" mass="14515" checksum="2966B4D876F7869B" modified="2012-04-18" version="1">MSMSDPIADFLTRIRNANMAHHESVEAPASKMKKDIAEILKNEGFIRDVEYVEDNKQGIIRVFLKYGKDGERVITGLKRISKPGLRSYVKADAVPKVLNGLGIAIVSTSNGVVTDKVARAQKVGGEVIAYVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIV6</accession>
    <name>H4GIV6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7 9">50S ribosomal protein L23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rplW</name>
        <name evidence="10" type="ORF">PS3_9725</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">One of the early assembly proteins it binds 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome. Forms the main docking site for trigger factor binding to the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 50S ribosomal subunit. Contacts protein L29, and trigger factor when it is bound to the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121989.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9725"/>
    <dbReference type="EnsemblBacteria" id="EHS87186">
        <property type="protein sequence ID" value="EHS87186"/>
        <property type="gene ID" value="PS3_9725"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.538"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_B">
        <property type="entry name" value="Ribosomal_L23_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_L23/L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001014">
        <property type="entry name" value="Ribosomal_L23/L25_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_L25/23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="PTHR11620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="SSF54189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00050">
        <property type="entry name" value="RIBOSOMAL_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="97" mass="11125" checksum="4863CAF99B372A06" modified="2012-04-18" version="1">MEARDIILRPVITEASTSMMDNKRYTFDVDLRATKTNVKNAVEEIFGVKVVKVNIMNVKGKQKRQGRYVGYTKRRRKAIVTLSADSNEIKLFGDDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIX7</accession>
    <name>H4GIX7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Hydroxyethylthiazole kinase</fullName>
            <ecNumber evidence="9">2.7.1.50</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">4-methyl-5-beta-hydroxyethylthiazole kinase</fullName>
            <shortName evidence="9">TH kinase</shortName>
            <shortName evidence="9">Thz kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">thiM</name>
        <name evidence="10" type="ORF">PS3_14588</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>5-(2-hydroxyethyl)-4-methylthiazole + ATP = 4-methyl-5-(2-phosphooxyethyl)-thiazole + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24212"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17957"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Thz kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.50" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122010.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14588"/>
    <dbReference type="EnsemblBacteria" id="EHS87149">
        <property type="protein sequence ID" value="EHS87149"/>
        <property type="gene ID" value="PS3_14588"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.572"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00139"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004417">
        <property type="term" value="F:hydroxyethylthiazole kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01170">
        <property type="entry name" value="THZ_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00228">
        <property type="entry name" value="Thz_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000417">
        <property type="entry name" value="Hyethyz_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02110">
        <property type="entry name" value="HK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000513">
        <property type="entry name" value="Thz_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01099">
        <property type="entry name" value="HYETHTZKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="266" mass="28512" checksum="52661C037F42898E" modified="2012-04-18" version="1">MTDFITELKATNPIVMTVANTITSGDVANGLNAIGVSPIMPSAPEEAEAMVNIAQGVTINVGSINQEQFRQSQLTCKAANSQGKPLVLDPVAVASSPYRSQVVDTLLRDYDFSAIRGNVGEIAFLAGVAWDTHGIDAGGDEVDFEQLTTIVKTCAQKYHTIVIMTGEIDYISDGKTVKSVPYGTPAFATHVGTGDLLSSLVAAFLTVASTDEYLEAAYQAALTFALCGQKVNDKLPGQWFNGLLNELYLVSNDKINHWEKEELHHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIY4</accession>
    <name>H4GIY4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Ornithine carbamoyltransferase</fullName>
            <shortName evidence="5">OTCase</shortName>
            <ecNumber evidence="3 5">2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_1540</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>carbamoyl phosphate + L-ornithine = H(+) + L-citrulline + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="3 5"/>
    <dbReference type="EMBL" id="AICN01000025">
        <property type="protein sequence ID" value="EHS87127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122017.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000025.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_1540"/>
    <dbReference type="EnsemblBacteria" id="EHS87127">
        <property type="protein sequence ID" value="EHS87127"/>
        <property type="gene ID" value="PS3_1540"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.583"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01109">
        <property type="entry name" value="OTCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024904">
        <property type="entry name" value="OTCase_ArgI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="OTCace_N" evidence="8">
        <location>
            <begin position="9"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="OTCace" evidence="7">
        <location>
            <begin position="155"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate binding" evidence="5">
        <location>
            <begin position="57"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate binding" evidence="5">
        <location>
            <begin position="135"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Ornithine binding" evidence="5">
        <location>
            <begin position="235"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate binding" evidence="5">
        <location>
            <begin position="272"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="340" mass="37556" checksum="594161CB55AAD6FE" modified="2012-04-18" version="1">MNNHFYGQSFLKELDFSAKELNYLIDFAIHLKALKQQHIPHPYLQGKNIALLFEKTSTRTRSSFTVAANDLGANPEFLGKNDIQLGNKESVADTAKVLGSMYDGIEFRGFKQSDVETLAQESGVPVWNGLTDDWHPTQMIADFMTLKEVYGHLPGLTLTYVGDGRNNMANSLLVTSAILGVNFKLGAPQHLQPAPAVISQAQALAQTSGAQITITDDPLTAVKDSDALYTDIWISMGETVDPAERIQALLPYQINQNLLNATGKDSTIVMHCLPAYHDKETTIGKQLAEEFGVSELEITNEVFLGQHSHVFQEAENRMHSIKAIMAATLGDLFIPEEFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIZ6</accession>
    <name>H4GIZ6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acetyl-CoA acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_2958</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2958"/>
    <dbReference type="EnsemblBacteria" id="EHS87108">
        <property type="protein sequence ID" value="EHS87108"/>
        <property type="gene ID" value="PS3_2958"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.600"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="5">
        <location>
            <begin position="8"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="6">
        <location>
            <begin position="277"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="3">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="395" mass="41780" checksum="A8CD5A0342D43EB2" modified="2012-04-18" version="1">MRLTMERVAIVGASRTPIGKLGGALASLSADQLGTIVIQAALNRAHVNPDQVDEVYLGCAIQAGAGQNVARQAAVNAGIPYSVPATTINVMCGSGLQAINLAAKMIQYGDADVMVAGGTESMSNAPYLIPKGRFGYKYGNAELIDAVYHDGLEDAFYHYPMGITAENLVQQYGITREQLDRFALESQRRAQAAQQAGRFKNEIVPVTLKTKHGSKTIMEDEAIRETSLGQLANLKAAFLEDGVVTAGNSSGINDGAAALVLMKESQAKTQHLSIMGYWEAGKLAGVDPQVMGIGPLVATKKVLETTNLTVDQLDLVELNEAFAAQSLIVKDQLNLPDDKVNVNGGAIALGHPLGDSGARIVVTLLYEMQRRQVNTGLATLCIGGGMGIAAIFNRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIZ7</accession>
    <name>H4GIZ7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidylglycerophosphatase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_11824</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122030.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11824"/>
    <dbReference type="EnsemblBacteria" id="EHS87109">
        <property type="protein sequence ID" value="EHS87109"/>
        <property type="gene ID" value="PS3_11824"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.602"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06971">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036681">
        <property type="entry name" value="PgpA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026038">
        <property type="entry name" value="Put_PGPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007686">
        <property type="entry name" value="YutG/PgpA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04608">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019587">
        <property type="entry name" value="PGPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101307">
        <property type="entry name" value="SSF101307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PgpA" evidence="1">
        <location>
            <begin position="59"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="165" mass="18830" checksum="E2EEF637411A06F6" modified="2012-04-18" version="1">MFNPNFKYPDTEAYNFVVNRLHERGVELHQLSDIAFEMQSQFGEHSSREEYYENLIDVLHKRELLNEAMVALELDRLAQENQIQEPLLSIIKNDSGVFGVDEALAIQISNIYGTIGTTNFGYLDRVKYGIIKKFDQDDQHVATFIDDLLGAIIAALCGKMAHLYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ06</accession>
    <name>H4GJ06_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">1-deoxy-D-xylulose-5-phosphate synthase</fullName>
            <ecNumber evidence="6">2.2.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PS3_8058</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the transketolase family. DXPS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.7" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122039.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8058"/>
    <dbReference type="EnsemblBacteria" id="EHS87118">
        <property type="protein sequence ID" value="EHS87118"/>
        <property type="gene ID" value="PS3_8058"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.619"/>
    <dbReference type="UniPathway" id="UPA00064">
        <property type="reaction ID" value="UER00091"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008661">
        <property type="term" value="F:1-deoxy-D-xylulose-5-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052865">
        <property type="term" value="P:1-deoxy-D-xylulose 5-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02007">
        <property type="entry name" value="TPP_DXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005477">
        <property type="entry name" value="Dxylulose-5-P_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43322">
        <property type="entry name" value="PTHR43322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13292">
        <property type="entry name" value="DXP_synthase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRANSKETOLASE_1" evidence="13">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS87118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="591" mass="65627" checksum="3C68A3A57D9E825A" modified="2012-04-18" version="1">MNLHPDYLLNRVEEPEDIKTMSMDQLEQLAEEMRTLILERVSTIGGHLGPDLGIVELAIAYHYVFNSPTDKVIWDVSHNSYPHKMLTGRKLGFLDPDHYQDISGFTAPDESDHDFFKIGHTSTSVALAVGEAEARDLKQDPKHRNIVAVIGDGSLSGGLAFEAFNNASKVKGNLIIVVNDNEMSIDDNQGGLYQGLAELRATEGKSPNNLFTFMGLDYRYIEDGNDLPTMIQMFKDVQDIDHPIVLHIHTLKGKGYQPAIDEKFKYHWRKAFDPTTGEDRQAPAQESYNQAVVKELLAEIDEGQEIAAINAAIPGAFGLKDFQAAQPDRYFDVGIAEQASITTAVGMAEAGIRPVVFENSTFLQRGLDQLIHDVALNSSPIVMVVVNGSISSQSATHQGTFDISYISSLPNIEYLAPTSVEEMVSMLRWAIHQQDVLVVIRQPAHELLHRPATTNDYDEVKYEIAHQGQDVALMAVGGFWKLGEQVQAQLKEKLNIDATLINPISVTNLDYETLHHLPEQHDVVVTIEDGNLSGGFGETIARYFGPTNVKVLSFGAPREFADAVPLDVLYNQYHLTPQQIVDDIQRVVHTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ17</accession>
    <name>H4GJ17_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable succinyl-diaminopimelate desuccinylase</fullName>
            <ecNumber evidence="5">3.5.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_5351</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + N-succinyl-(2S,6S)-2,6-diaminoheptanedioate = (2S,6S)-2,6-diaminoheptanedioate + succinate</text>
            <dbReference type="Rhea" id="RHEA:22608"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:58087"/>
            <dbReference type="EC" id="3.5.1.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 3/3.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.18" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000027">
        <property type="protein sequence ID" value="EHS87093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122050.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000027.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5351"/>
    <dbReference type="EnsemblBacteria" id="EHS87093">
        <property type="protein sequence ID" value="EHS87093"/>
        <property type="gene ID" value="PS3_5351"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.638"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00021"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009014">
        <property type="term" value="F:succinyl-diaminopimelate desuccinylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010182">
        <property type="entry name" value="ArgE/DapE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01910">
        <property type="entry name" value="DapE-ArgE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="8">
        <location>
            <begin position="171"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="379" mass="41284" checksum="ED0CEAFC1B5FDEB5" modified="2012-04-18" version="1">MDEERALKILQDLIRLQTVNGNEAQAAHYLQEILRQAGARVEMVDFAPERSGLVAEIGPTTGPGLALAGHLDTVAIGDPASWHHDPFGAEVENGRIYGRGSVDMKGGLAAMVATMLALAEEVDQLKGRLRLLISVDEEVGGQGSLQLTQLGYVHDVDAMIVCEATDDQIQYAHCGSFDYQIDSQGKLAHSSRPQLGANAVMNLVDYINQEVHVFDDHPTSPVLGELVHSVTVFHGGEQLNSIPAKAYLQGNVRTIPECDNQATKDRLQAIIDDLNQDPKHQLSLSIVADFMPVVTDPNDPFIGQLQDIVEAVTGQRPTATISHGATDASRYVMDDHQFAVVEYGPGSEDQSHQVDESLALEDYYQAITVYQKLAHDFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ19</accession>
    <name>H4GJ19_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">L-2-hydroxyisocaproate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_3004</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. LDH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000027">
        <property type="protein sequence ID" value="EHS87095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122052.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000027.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3004"/>
    <dbReference type="EnsemblBacteria" id="EHS87095">
        <property type="protein sequence ID" value="EHS87095"/>
        <property type="gene ID" value="PS3_3004"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.641"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018177">
        <property type="entry name" value="L-lactate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="SSF56327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00064">
        <property type="entry name" value="L_LDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ldh_1_N" evidence="6">
        <location>
            <begin position="4"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Ldh_1_C" evidence="7">
        <location>
            <begin position="148"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="281"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="311" mass="33787" checksum="971292581149A278" modified="2012-04-18" version="1">MSRKIGIVGMGNVGAAAAHYIVANGFADQLVLIDKNEAKVKADALDFEDAMSNLQYHTDITVNDYSALEDADVVISAIGSIKLQDNAGDDRFAELPFTRVNAREVAENLKAAGFHGKLVVITNPVDVITSIYQQVTGLPKNHVIGTGTLLDSSRMKRAVASRLGLDPRSVVGYNLGEHGNSQFTAWSTVRVLGKPITELAEARGLDLEELDHEAKVGGHTVFHGKKYTNYGVSTAAVKLALTIMSNSLTEMPVSNYREEYGIYLSYPAVVGNDGIVEQLQLDLTQEELDKLQNSANFIKQKYAESQEALKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ22</accession>
    <name>H4GJ22_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Aminopeptidase</fullName>
            <ecNumber evidence="8">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PS3_11892</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.</text>
            <dbReference type="EC" id="3.4.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase M1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000027">
        <property type="protein sequence ID" value="EHS87098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122055.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000027.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11892"/>
    <dbReference type="MEROPS" id="M01.002"/>
    <dbReference type="EnsemblBacteria" id="EHS87098">
        <property type="protein sequence ID" value="EHS87098"/>
        <property type="gene ID" value="PS3_11892"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.646"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="SSF63737"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 8" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M1_N" evidence="11">
        <location>
            <begin position="11"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M1" evidence="9">
        <location>
            <begin position="216"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1_C" evidence="10">
        <location>
            <begin position="507"/>
            <end position="814"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="843" mass="95621" checksum="0B83986F9D4B878F" modified="2012-04-18" version="1">MAISRFYDLFQPENYQIKLAIDRQAKTITGQVTITGHASQTDLQLHQKFLQVSQVLVNQNPVDFTSDDEQESLQFTSPSTGELTITVNYTAPLTDTMMGIYPSYYQIGDERKQLIGTQFETTFARQAFPSIDEPEAKATFDLAIKFDEQPGETILANMPETKVVDGYHYFQRTVKMSTYLVAFAFGDLQSKLTQTKSGVQVGVFATKAHAAKELDFALDIAKRAIEFYEDYYQTPYPLPHSWQLALPDFSAGAMENWGLVTYREAYLLLDPDNTALEMKQVVATVITHELAHQWFGDLVTMQWWDDLWLNESFANMMEYVAVDALEPDWHVWDLFQTTEVPMATNRDATDGVQSVHVDVNNPAEIDSIFDGAIVYAKGSRMLVMVRSLIGDDALRKGLKNYFAAHQYGNAKGADLWEALGQAANMPIGDIMNSWLEQPGYPVVQAKVENGHLILKQAQFFIGEHQDQGRQWQIPLNSNYAGVPKLMTTESLDLGDYQSLRQTAGKPFLLNQDNQAHFIVQYDTELFNDLLAQVDQLSNSDQREFLQDLSLLAQGQYISYADLIPRLGQFANQSSYIVTDVLYQTANKLKQFIQAGQAEEKYLQDFFKVLSQDQLEQLGWTANQQDTNDQILMRPTIISAALYGHNQVAIRQAHDLFAEYHDHLVDLPADTRGAIIKNELQHYLSAEVFHELLNTYRTTTDPSFKVALRGALTSITDADLIQHLIGEFENAETIKPQDLRGWFQGLLANEFAQQYAWDWIRNDWDWLEATVGGDMEFATYITVISRVFKTADRLAEFKAFFEPKLNTPGLTREIEMDTKVIASRVALVQAEKADVLAALQAYNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ39</accession>
    <name>H4GJ39_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_5712</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122072.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87064">
        <property type="protein sequence ID" value="EHS87064"/>
        <property type="gene ID" value="PS3_5712"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.678"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="390"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="447" mass="48432" checksum="17EBF4A01846921A" modified="2012-04-18" version="1">MDNKSTTSGLPMLSRSMIWMINFGFLGVQTAFTLQSSQMSRIFQTLGADPNNLGWFFILPPLAGIIVQPIVGYYSDRTWAPKLGGRRLPYLILGTIVAVIVMILLPNSGSFGFGYGSLAALWFGAVTVAFLDLSSNVAMQPFKMMVGDMVNDKQKSYAYGIQSFLSNTGAVLAAIFPFLLTWFGVANTAKKGVVPQSVVIAFYVGAALLVVTSLFTILRVKEYDPATYAKYHGISESDNSEGGNWLTLLKQAPKAFWTVTLVQFFCWFAFQYLWTYSAGAIAQNVWHTTNATSAAYQAAGNWYGVLAAVQSIAAVVWSYVLAKVPNQYHKVGYAGSLLLGAVGFLSVFFIHNQYALIISYALVGIAWAGMNTYPLTIVTNALSGKHMGTYLGLFNGSICLPQIVASVFSFALFPMLGGSQVNMFLLAGIVMVIGALSVATIKETHQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ46</accession>
    <name>H4GJ46_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit MurT</fullName>
            <ecNumber evidence="1">6.3.5.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murT</name>
        <name evidence="4" type="ORF">PS3_15372</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The MurT subunit catalyzes the ATP-dependent amidation of D-glutamate residue of lipid II, converting it to an isoglutamine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H2O + L-glutamine = ADP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57928"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:62233"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = ADP + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:59488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:143132"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + NH4(+) = beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:62233"/>
            <dbReference type="ChEBI" id="CHEBI:143132"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a heterodimer with GatD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MurCDEF family. MurT subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.13" evidence="1"/>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122079.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15372"/>
    <dbReference type="EnsemblBacteria" id="EHS87071">
        <property type="protein sequence ID" value="EHS87071"/>
        <property type="gene ID" value="PS3_15372"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.685"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140282">
        <property type="term" value="F:carbon-nitrogen ligase activity on lipid II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02214">
        <property type="entry name" value="Lipid_II_synth_MurT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043703">
        <property type="entry name" value="Lipid_II_synth_MurT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013564">
        <property type="entry name" value="MurT_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08353">
        <property type="entry name" value="MurT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0133">Cell shape</keyword>
    <keyword evidence="1" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="2">
        <location>
            <begin position="54"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="MurT_C" evidence="3">
        <location>
            <begin position="319"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="445" mass="49669" checksum="190AEF67EA1F09BD" modified="2012-04-18" version="1">MSIRSSIAEIAGRSSYWFLHSFFHGGSSLPGKITTRIDPNILKAFSQKYDVVIVTGTNGKTLTTALSVRVAQEKYQQVLTNPTGSNMTQGIVTTFITAPKAKQKGLAVIEVDEAYVVQITRQITPIAFVFTNLFRDQMDRYGEIYTTYDKILKGVALAPHATIIANGDDQIFNTKELPNPIIYFGFANNSSADLQAPANTDGLLCPKCHHILHYHKIVYANLGDYYCPHCGFKRPELTYQVTDIKQLTPTSSEVEIDGDAYHIGIGGVYNIYNALAAYSLGRFLGVPAEQIQHAFASDKKVFGRQEVIPIDDKEVTLILVKNPVGLNQVLDMILTDPEPFSFGFLLNANYADGIDTSWIWDGDFEKLLDHDIPQFMTGGERYKDISTRLTMAGIDEMWTCPDLNQVIDQIKQMPTKHVYLLATYTAVLQVRKLLADEHYIQGGMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ55</accession>
    <name>H4GJ55_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flavoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13250"/>
    <dbReference type="EnsemblBacteria" id="EHS87080">
        <property type="protein sequence ID" value="EHS87080"/>
        <property type="gene ID" value="PS3_13250"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.699"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="220" mass="25436" checksum="69F4325305C4B48A" modified="2012-04-18" version="1">MAKEDFAAKGQGKFTVNGLNPHDDDWLEKASQKVHAAQGDDYVLLDRGLLTVNQLNWLLKEVYGELTYCDDNHQLMWYNRQYDDPNQMMGRRRPEQVGDNMDEVHPDVGNVRKYAKQVWYGLRTKADGRDEVWTPVSKGHGEPIMHYNRYKRIEDEAGNFRGIAEWVVDLKPLAEYYCRTNGLKMVPDDQAQRPDFVETPADVKRHQADKDIDAASHPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ61</accession>
    <name>H4GJ61_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_7159</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122094.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7159"/>
    <dbReference type="EnsemblBacteria" id="EHS87086">
        <property type="protein sequence ID" value="EHS87086"/>
        <property type="gene ID" value="PS3_7159"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.708"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024528">
        <property type="entry name" value="ThrE_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12821">
        <property type="entry name" value="ThrE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="ThrE_2" evidence="6">
        <location>
            <begin position="9"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="166" mass="18252" checksum="98A0A7F810636BD4" modified="2012-04-18" version="1">MQTNLLIQCSMAFIAAICFGMMLNIPVKAYLPSGLIGCLSWAVYVILYFQLHLGLAMSNMIGAILISVTSMLLARRQKLPMIIYNVPALVNFVPGGQSYRMISNFVLGHNALAMTYFYQVIVIAGALSLGFGIGDLLNAAIFGRRIRGPRPFSQVLLPKKRPPKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ72</accession>
    <name>H4GJ72_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Exopolyphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_4593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122105.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4593"/>
    <dbReference type="EnsemblBacteria" id="EHS87027">
        <property type="protein sequence ID" value="EHS87027"/>
        <property type="gene ID" value="PS3_4593"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.721"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003695">
        <property type="entry name" value="Ppx_GppA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02541">
        <property type="entry name" value="Ppx-GppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ppx-GppA" evidence="1">
        <location>
            <begin position="24"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="309" mass="34827" checksum="D537C68AF59A6EA6" modified="2012-04-18" version="1">MINFVVIDLGSNSVRMRINELMENGSFLLTHQVKEYVRLSENMGPDKVLQKEPMERTIKALQEFKEIYDQLPNPQVHAIATAAVRQAKNQKHFLKRVKEAVDLEFDVISGDQEAYLDYLGVTRTLPVNDCIIMDTGGASTELVYVEGGKMKDRISIPLGSVLISQRFGLDDEISAVSLFKAMTFVEQALAKIDWLQEAHGKQLIGLGGSNRTLAKIMRRQLALDPDDLPDIHGMHVPSAHVYELMESVIQMNRTQREKTPGLAKVRADVIVGGWMPIILVMRMLDIQEVVFSNHGLREGVLYDYLDHQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ76</accession>
    <name>H4GJ76_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">L-lactate dehydrogenase</fullName>
            <shortName evidence="6">L-LDH</shortName>
            <ecNumber evidence="4 6">1.1.1.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ldh</name>
        <name evidence="12" type="ORF">PS3_1302</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of lactate to pyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>(S)-lactate + NAD(+) = H(+) + NADH + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:23444"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the LDH/MDH superfamily. LDH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.27" evidence="4 6"/>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122109.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_1302"/>
    <dbReference type="EnsemblBacteria" id="EHS87031">
        <property type="protein sequence ID" value="EHS87031"/>
        <property type="gene ID" value="PS3_1302"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.729"/>
    <dbReference type="UniPathway" id="UPA00554">
        <property type="reaction ID" value="UER00611"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00488">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011304">
        <property type="entry name" value="L-lactate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="SSF56327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01771">
        <property type="entry name" value="L-LDH-NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ldh_1_N" evidence="10">
        <location>
            <begin position="7"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Ldh_1_C" evidence="11">
        <location>
            <begin position="147"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="122"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="150"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6 7">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="67"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="81"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 8">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
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    <feature type="binding site" evidence="6">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
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        <location>
            <begin position="120"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
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        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphotyrosine" evidence="6">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00488"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="316" mass="33757" checksum="2ED4CAD213687344" modified="2012-04-18" version="1">MSKNHQKVVLIGDGAVGSSYAFAMVQQGLAEEFAIIDINKDRIHGDALDLEDATPFTSPKLVYDADYDTCGDADLVVITAGVPQKPGETRIELVDKNLKIMKSVVEPIMASGFDGIFLVAANPVDILTYAVQKFSGFPKNKVVGSGTSLDSARLRVALAKKFGVASPDVMANMMAEHGDTEFAAYSSATIGGKPLLEMAQEAGVTDEELLQIEDDVRHKAYEIINTKGATYYGVATCLMRISRAILRDQNAVLPVGAPMNGEYGLEDIYIGTPAVINASGVARVIEVPLNEREQKAMADSAKALKEVATNGLAKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ91</accession>
    <name>H4GJ91_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_21948</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="EHS87046">
        <property type="protein sequence ID" value="EHS87046"/>
        <property type="gene ID" value="PS3_21948"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.755"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="247" mass="27759" checksum="2037081B789FA2AD" modified="2012-04-18" version="1">MLHQLAPKDPLKLPGNAEQYAVKNVVLNPDSPLKGKRIYFLGSSITLGAAANDESFVDYLVAEDGIIAQKEALSGTTLANSNQPLLKGNSYLERLAKNPPKQPLDLMVVQLSTNDGRKQIPLGQISSTKDMATFDQQTTLGALEYLCALVTQNWVCPLAFYTCLRKGNDYQILIAKLKVLQDKWGFSMIDLGSDEKIISVTQQHPSYMYDDAHPTRLGYRDLWTPYFRKELLSIFKDQEVKLWSMQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ94</accession>
    <name>H4GJ94_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_16408</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000030">
        <property type="protein sequence ID" value="EHS87019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122127.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000030.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16408"/>
    <dbReference type="EnsemblBacteria" id="EHS87019">
        <property type="protein sequence ID" value="EHS87019"/>
        <property type="gene ID" value="PS3_16408"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.759"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="172" mass="20358" checksum="9743FCD40B478547" modified="2012-04-18" version="1">MLDITKLLQSSISFDRQMTEQKDILLGPEARQINAMTTLDIELGKLYQLSHWNEVWHDQETENLTELTRQFVRVIFWLLLFSAQKQWTHLVVMTPADYEHIKQLPPQSKVADQDKLYLSIKNFLYGAYFEHRQADFKHAWHLLLKLGLVDLKLDDQKIMTSFAEWQAALLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJF8</accession>
    <name>H4GJF8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphinothricin N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000037">
        <property type="protein sequence ID" value="EHS86719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122191.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000037.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13238"/>
    <dbReference type="EnsemblBacteria" id="EHS86719">
        <property type="protein sequence ID" value="EHS86719"/>
        <property type="gene ID" value="PS3_13238"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.908"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="165" mass="18793" checksum="2262EACEA315EDE9" modified="2012-04-18" version="1">MTIKFELATNKDLPKIVEIYNEAIPTHQSTADLEPITIDDRIDWFNSFDDTHPIWLIKANDETVGWVALEQMYGRAAYQKSAEIAIYLSPNGQGKGIGPQAIQFLKDQAKELGLKAIAAYVLSQNLPSRKMFERSGFAEWGHLPKVCIFDDIERDIYIYGYHPND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJG3</accession>
    <name>H4GJG3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_4939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000038">
        <property type="protein sequence ID" value="EHS86717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122196.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000038.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4939"/>
    <dbReference type="EnsemblBacteria" id="EHS86717">
        <property type="protein sequence ID" value="EHS86717"/>
        <property type="gene ID" value="PS3_4939"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.915"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="301" mass="34546" checksum="CA8FAE4D98BF6C47" modified="2012-04-18" version="1">MKPICAIVVTYNRKKLLRESIESLQNQTVNNFDILVVDNASTDNTKEYISDLIDSQQILYENTGANLGGSGGFNFGLKAACERGYQYVWLMDDDTMPTPTALAELLKADQLLDGNYGYLASEVLFTDGQPCKMNGQKIYKKWPDESQYIKDGLVRTIQATFVSFFLKTSTIEEVGLSYKEFFIWGDDMEYSNRISKRHPSYLVGKSIVIHKTKDNNGSNLSKDDGSKLWRYKLAYRNECVIARSNGLYGRCFQFAKVNWNIIKVLRSDQPYKLKKIWIIISSSIRGIFFRPKIEYVKVNRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJH3</accession>
    <name>H4GJH3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_6502</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000039">
        <property type="protein sequence ID" value="EHS86616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122206.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000039.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6502"/>
    <dbReference type="EnsemblBacteria" id="EHS86616">
        <property type="protein sequence ID" value="EHS86616"/>
        <property type="gene ID" value="PS3_6502"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.924"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="206" mass="23490" checksum="B4B01B21E4AADAB5" modified="2012-04-18" version="1">MSWNWLGWLLWIAAIAFLVYVIHYIRVKQLMLIAKTHQRFEKGLFFRYVVLVVIALAWLGFMGYATWFRPVSYADSSAVTTKTTYTALQLSSSGNDYYYVRVNHSNAGNHGVVSYTYQTQDGNYTTSAYFSSLADGNNVLPATAMDLPWNKKKLNQTDTQTGHAFVATMTVRYKNTLMNGIGIRANHIAIQYNLIRIPSDDMEIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJI7</accession>
    <name>H4GJI7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Anaerobic ribonucleoside-triphosphate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_13775</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000041">
        <property type="protein sequence ID" value="EHS86577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122220.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000041.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13775"/>
    <dbReference type="EnsemblBacteria" id="EHS86577">
        <property type="protein sequence ID" value="EHS86577"/>
        <property type="gene ID" value="PS3_13775"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.946"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008998">
        <property type="term" value="F:ribonucleoside-triphosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01675">
        <property type="entry name" value="RNR_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019777">
        <property type="entry name" value="Form_AcTrfase_GR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001150">
        <property type="entry name" value="Gly_radical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012833">
        <property type="entry name" value="NrdD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13597">
        <property type="entry name" value="NRDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02487">
        <property type="entry name" value="NrdD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00850">
        <property type="entry name" value="GLY_RADICAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51149">
        <property type="entry name" value="GLY_RADICAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 5" id="KW-0556">Organic radical</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="7">
        <location>
            <begin position="18"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Glycine radical" evidence="6">
        <location>
            <begin position="592"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="modified residue" description="Glycine radical" evidence="5">
        <location>
            <position position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="720" mass="81974" checksum="DD1D74EAF7AFC7C2" modified="2012-04-18" version="1">MQSVETQSVTKTLKPSTWMVIKRDGSQFNFHEYKIKAVLDQLGLSKADQAEVMGNLETQLAALSEISTIAISKVLVKVLREHNFKQAAESYVAYRLQDELDWKNQVDPVQRINRLVANEDTLVHENANKDSKVFNTQRDLTAGAVGKTVGLKLMPEHVAKAHVRGDIHYHDLDYTPLSPMTNCCLIDFKEMLTNGFKIGNAEVESPKSIQTATAQMAQIIANVASSQYGGCSADRVDEVLEPFAKCNYEKHLKESAEFIDDEDKQKAFAVKRTKKDIYDAMQGLEYEINTLFSSQGQTPFTTLGFGLGTSWIAREIQKAILQVRIEGLGKEKRTAIFPKLVFTIKKGLNYAPDEPNYDIKQLAVECATKRMYPDILMYDKIIELTGSFKAPMGCRSFLQGWWDEDGREVNSGRMNLGVVTINLPRIAMESHGDMDLFWEILNERLQVAKDALHFKVERTKEALPENAPILYRYGAFGKRLREDESVDELFKNSRATVSLGYIGLYEVGTVFYGPNWEHNQAAHDFTVEIVKVLHDKCVEWERESGYHYSVYSTPSESLTDRFCRLDTKKFGKVKDITDKEYYTNSFHYDVRKHPTPFEKLNFEKDYPKYAAGGFIHYCEYPNLKQNPAALEAVWDWAYDKVGYLGTNTPIDHCYKCGFQGEFKATAKGFQCPDCGNHDPKTCDCVKRTCGYLGNPLQRPMVHGRHVEIASREKNMRLANE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJL0</accession>
    <name>H4GJL0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metal uptake regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_15351</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fur family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122243.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15351"/>
    <dbReference type="EnsemblBacteria" id="EHS86520">
        <property type="protein sequence ID" value="EHS86520"/>
        <property type="gene ID" value="PS3_15351"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.988"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043135">
        <property type="entry name" value="Fur_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="PTHR33202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="147" mass="17009" checksum="CAC76EF2C4B0F57F" modified="2012-04-18" version="1">MATLSEQAVALIRRSQLRLTKQRQSLIDILASSPERYLEVTYVDQLMRQQYPGVSHNTIYRNLEEFQTAGIVEITQRSHGKAVKLSCDQEAHEHHHHFICQECGRVTEIHMKPFDLEFYQAQLPGAEITGHEFELYGRCADCAKKHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJM6</accession>
    <name>H4GJM6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphomevalonate kinase</fullName>
            <ecNumber evidence="2">2.7.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_20424</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 2/3.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.2" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000043">
        <property type="protein sequence ID" value="EHS86441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122259.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000043.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20424"/>
    <dbReference type="EnsemblBacteria" id="EHS86441">
        <property type="protein sequence ID" value="EHS86441"/>
        <property type="gene ID" value="PS3_20424"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1005"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00099"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004631">
        <property type="term" value="F:phosphomevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035102">
        <property type="entry name" value="Phosphomevalonate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005917">
        <property type="entry name" value="Pmev_kinase_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31814">
        <property type="entry name" value="PTHR31814"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01220">
        <property type="entry name" value="Pmev_kin_Gr_pos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="7">
        <location>
            <begin position="109"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP_kinases_C" evidence="8">
        <location>
            <begin position="260"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS86441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="364" mass="40232" checksum="27A5A47CD8D183B6" modified="2012-04-18" version="1">MIIAKAPGKLYIAGEYAVVENQYPAVLVALNQFVTCTIRPTSHLEGSITSHQYENMGLSWRRNGDQMVIDRRDNPFVYILSAISVTEEYAKALHKPLGVYDLTIDSQLDSDSGKKYGLGSSAAVTVATVKALLNYYEIPASKEVIFKLASIAHFTIQGNGSLGDVAASVYGGWIAYRSFDRAWLANQRQRCTLVDLISQTWPGLEIESLIVPNELQLMIGWTGKPASTSHLVDKIDAFHQIHPEQYQEFLENSRQCVERMVDGFHRQDIATIQNELRNNRLLLQSLAIQSEVQIETPLLQQLCQIAEQFKGAAKTSGAGGGDCGIAILSHPKDRSRLTAAWQAAGIEPLNLAIHYVDEVISESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJP5</accession>
    <name>H4GJP5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="7">DMRL synthase</shortName>
            <shortName evidence="7">LS</shortName>
            <shortName evidence="7">Lumazine synthase</shortName>
            <ecNumber evidence="4 7">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ribH</name>
        <name evidence="8" type="ORF">PS3_3331</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2 H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the DMRL synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="4 7"/>
    <dbReference type="EMBL" id="AICN01000045">
        <property type="protein sequence ID" value="EHS86246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122278.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000045.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3331"/>
    <dbReference type="EnsemblBacteria" id="EHS86246">
        <property type="protein sequence ID" value="EHS86246"/>
        <property type="gene ID" value="PS3_3331"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1034"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09209">
        <property type="entry name" value="Lumazine_synthase-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="PTHR21058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="SSF52121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="5-amino-6-(D-ribitylamino)uracil binding" evidence="7">
        <location>
            <begin position="56"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-amino-6-(D-ribitylamino)uracil binding" evidence="7">
        <location>
            <begin position="80"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="1-deoxy-L-glycero-tetrulose 4-phosphate binding" evidence="7">
        <location>
            <begin position="85"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="154" mass="17106" checksum="E17079FC4C07234F" modified="2012-04-18" version="1">MTIYEGHFNDQDYRIAIVISRFNEAITKRLLDGAVDNLVRHGVAKDRIDVYWVPGAYEIPFMAKKVVQSERYDGLVTLGAVIRGETSHYDLVTNGVANGVLELNLTSSIPVTFGVVTTENVDQAMQRSRVKAGNKGYDTAQALLEMISLSRQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJP9</accession>
    <name>H4GJP9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative (R,R)-butanediol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_4374</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000045">
        <property type="protein sequence ID" value="EHS86250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122282.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000045.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4374"/>
    <dbReference type="EnsemblBacteria" id="EHS86250">
        <property type="protein sequence ID" value="EHS86250"/>
        <property type="gene ID" value="PS3_4374"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1040"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027399">
        <property type="entry name" value="ADH_N_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13823">
        <property type="entry name" value="ADH_N_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PKS_ER" evidence="4">
        <location>
            <begin position="8"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="350" mass="36995" checksum="6F11B1C78D4A94B2" modified="2012-04-18" version="1">MKAAVWHGAKDIRVKDVDLKPLQDNEVTVKVAWTGICGTDLHEYLEGPVYVPTEGPDPLLGGQAPITLGHEFAGVIERIGKKVTNYQVGDHVAINPTVTNADRPSDIDVYSGYSFIGLASDGGFASHANIPEDNLVRLPKNFPLRLGAVIEPAAVAVQAIKRNGLTFGQNVAIFGAGPIGCLVAAAAKAAGANRIIICDLSPQRLAKALELGATDTINSGEQDPVQRIKELVPGGVDASFEVAGVQVTFNQAILATKPLGTMTLVSIFGHPIEFNPMILTNTGVKITSTIAYSQATFLETAELITKGSLQVEPVITKEIPLDDIVTDGFEALTTDKSQAKILVNLDGLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJQ8</accession>
    <name>H4GJQ8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_6160</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000047">
        <property type="protein sequence ID" value="EHS86208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122291.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000047.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6160"/>
    <dbReference type="EnsemblBacteria" id="EHS86208">
        <property type="protein sequence ID" value="EHS86208"/>
        <property type="gene ID" value="PS3_6160"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1061"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039369">
        <property type="entry name" value="LacA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024688">
        <property type="entry name" value="Mac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43017">
        <property type="entry name" value="PTHR43017"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12464">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01266">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Mac" evidence="4">
        <location>
            <begin position="5"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="204" mass="22467" checksum="B16F868E356BE515" modified="2012-04-18" version="1">MDTNRQKMLNGRPYDPSSPDLAKHRQQTHELCLEYNQTPESDVQKRTEIITTLVPHQGAGAYFQGPIQFDYGDNITVGQNFYANFNLTILDVCPVKIGDNVMLGPNVTIATPLHPLTFQQRNVRLQADGRETNYEYGQPITIGDNCWLAANVTVCPGVTIGEGCVIGAGSVVTKDLPDNCLAVGSPARVIRQITADDRLTDFPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJT3</accession>
    <name>H4GJT3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Branched-chain amino acid transport system carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_10761</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the transport system for branched-chain amino acids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the branched chain amino acid transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122316.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS86120">
        <property type="protein sequence ID" value="EHS86120"/>
        <property type="gene ID" value="PS3_10761"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1110"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004685">
        <property type="entry name" value="Brnchd-chn_aa_trnsp_Livcs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30588">
        <property type="entry name" value="PTHR30588"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05525">
        <property type="entry name" value="Branch_AA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00796">
        <property type="entry name" value="livcs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="154"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="240"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="353"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="417"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS86120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="456" mass="49529" checksum="2DB1FB7B484536DC" modified="2012-04-18" version="1">MKTSQLNWKKYLLLGSLIFGMFFGAGNLIFPIHLGQLAGSHWLSAAIGFIFSGVLLPLMALLAISITRSEGIYDLALPNGKWFALIFLILVHATLGPLFATPRTATVPFSIGIAPHLAKSLAGPGLFIYSAIFFGIVYFFSTREGNITTIIGKILNPLFLLLLFVIFLLAFISPLGSATTTTATSAYLTSSFTNGFLEGYNTMDCLAALAFGITVITAIKNYGLSRQRDISLATAKSGSIGILGIALIYLALIFLGATSLHHFTLAENGGTTLAQIAHYYLGTAGDAILATLATVTCMTTAMGLVIAFSQDFHKRFPRISYKAFLRFNCGLSFLIANLGLDQIIAWSTPMLMFLYPLAIALIILAIFSPFFKNDPMIYRITMAFTLIPAIFDMINSAPEIIRNTGFAQAMINFASHYFPFFSLGFGWLTFGIAGLIIGLLTHIFKTRQALPSLEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJT7</accession>
    <name>H4GJT7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UPF0122 protein PS3_3228</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_3228</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Might take part in the signal recognition particle (SRP) pathway. This is inferred from the conservation of its genetic proximity to ftsY/ffh. May be a regulatory protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the UPF0122 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122320.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3228"/>
    <dbReference type="EnsemblBacteria" id="EHS86124">
        <property type="protein sequence ID" value="EHS86124"/>
        <property type="gene ID" value="PS3_3228"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1115"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00245">
        <property type="entry name" value="UPF0122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007394">
        <property type="entry name" value="UPF0122"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40083">
        <property type="entry name" value="PTHR40083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04297">
        <property type="entry name" value="UPF0122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="113" mass="13558" checksum="257F340EFA843424" modified="2012-04-18" version="1">MELEKNERVNALFAFYQPLLTSKQNDYLQLYYADDYSLGEIAEEFQVSRQAVYDNIRRTERILEDYEQKLHLFSNFQLRNQQADQIQTYIKEHYPDDQNLNQLVAGLENLEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJT8</accession>
    <name>H4GJT8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Signal recognition particle receptor FtsY</fullName>
            <shortName evidence="5">SRP receptor</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ftsY</name>
        <name evidence="8" type="ORF">PS3_18555</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GTP-binding SRP family. FtsY subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122321.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18555"/>
    <dbReference type="EnsemblBacteria" id="EHS86125">
        <property type="protein sequence ID" value="EHS86125"/>
        <property type="gene ID" value="PS3_18555"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1116"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031226">
        <property type="term" value="C:intrinsic component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00920">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004390">
        <property type="entry name" value="SR_rcpt_FtsY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF7">
        <property type="entry name" value="PTHR43134:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="SSF47364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00064">
        <property type="entry name" value="ftsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54" evidence="7">
        <location>
            <begin position="405"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="238"/>
            <end position="245"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="320"/>
            <end position="324"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="384"/>
            <end position="387"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="438" mass="47534" checksum="CE105E2CD7CF71E8" modified="2012-04-18" version="1">MGLFDIFRRKAKPSSEASESTVVSESTTESLVETSNSQTEASVSEASRVNEGSLTTSESETILASESQSTSLSENSQVSLASISESELAASEVSEQSLEASTSESESTEPATSETPNETVEETDTATTEEKYDQGLKKSRSGFGERLNRFLARFRSVDEDFFDDLEELMIESDVGYEMAVRLSGELREEVKLQNAKSTQEVSNVIIEKMVELYEEAGANEDDSIHLAEQGPTVIMFVGVNGAGKTTTIGKLAARYKQAGQKVLLAAADTFRAGATEQLEVWAQRDGVDIVTGPENSDPAAVVFDAVKKAKDDHYDILFVDTAGRLQNKVNLMNELAKMKRILTREIPSAPHEVLLVLDATTGQNALNQAKLFKESTDVTGIVLTKLDGTARGGIVLAIRNELHLPVKFVGLGEKVTDLEKFDASDFVYGLFKGLVNVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJU2</accession>
    <name>H4GJU2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Phosphate acyltransferase</fullName>
            <ecNumber evidence="3 4">2.3.1.274</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Acyl-ACP phosphotransacylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Acyl-[acyl-carrier-protein]--phosphate acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Phosphate-acyl-ACP acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">plsX</name>
        <name evidence="5" type="ORF">PS3_1742</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reversible formation of acyl-phosphate (acyl-PO(4)) from acyl-[acyl-carrier-protein] (acyl-ACP). This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>a fatty acyl-[ACP] + phosphate = an acyl phosphate + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:42292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.274"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Probably interacts with PlsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Associated with the membrane possibly through PlsY.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PlsX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.274" evidence="3 4"/>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122325.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_1742"/>
    <dbReference type="EnsemblBacteria" id="EHS86129">
        <property type="protein sequence ID" value="EHS86129"/>
        <property type="gene ID" value="PS3_1742"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1120"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043811">
        <property type="term" value="F:phosphate:acyl-[acyl carrier protein] acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00019">
        <property type="entry name" value="PlsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003664">
        <property type="entry name" value="FA_synthesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012281">
        <property type="entry name" value="Phospholipid_synth_PlsX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30100">
        <property type="entry name" value="PTHR30100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02504">
        <property type="entry name" value="FA_synthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002465">
        <property type="entry name" value="Phsphlp_syn_PlsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00182">
        <property type="entry name" value="plsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="341" mass="36688" checksum="55D3D071DBF95EEB" modified="2012-04-18" version="1">MKIAVDAMGGDQAPQVVVAGVEQARDLYPELEFDLYGDPQQVTPLIQDSTRLKIVPTSEIIEMGEEPVRAIRKKKDSSIAQAATAVKEGRADAFFSAGNTGAILAAGLLIVGRIKGIDRPGLTSILPITQPGSDQTRFVYLDTGANAESKTKNLLQYAIMGKFYAENVLKVANPRVALLNNGAEEDKGDSFHKEVHQMLKQTADLNFVGNVEAGDLLYGKADVIVSDGWTANAALKATEGTAKMFMNLIKDGVKQGGWRALLGAWLLKPALKRIKNQMSTSTYGGAVLLGLKAPVVKTHGSSDAEAIKNTIVQIKQMIDANLIGQTVDYFSADQTREKLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJU5</accession>
    <name>H4GJU5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alkaline shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_15382</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asp23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122328.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15382"/>
    <dbReference type="EnsemblBacteria" id="EHS86132">
        <property type="protein sequence ID" value="EHS86132"/>
        <property type="gene ID" value="PS3_15382"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1123"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005531">
        <property type="entry name" value="Asp23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34297">
        <property type="entry name" value="PTHR34297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03780">
        <property type="entry name" value="Asp23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="120" mass="12933" checksum="F500673818FD4437" modified="2012-04-18" version="1">MTVKIKNQSGMIDISNDVIATIVGGAATDIYGVVGMASRKPVRDGFNQILGLDSYHQGVEVRQNDDQIDVEVNIIVSYGTKISEVSKSVQSQVKYNLKSMLGITADEVNVFVQGVRVAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJV1</accession>
    <name>H4GJV1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">16S rRNA (cytosine(967)-C(5))-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.176</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">16S rRNA m5C967 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">rRNA (cytosine-C(5)-)-methyltransferase RsmB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PS3_12675</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cytidine(967) in 16S rRNA + S-adenosyl-L-methionine = 5-methylcytidine(967) in 16S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42748"/>
            <dbReference type="Rhea" id="RHEA-COMP:10219"/>
            <dbReference type="Rhea" id="RHEA-COMP:10220"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74483"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.176"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.176" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12675"/>
    <dbReference type="EnsemblBacteria" id="EHS86138">
        <property type="protein sequence ID" value="EHS86138"/>
        <property type="gene ID" value="PS3_12675"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1129"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="Fmu/NOL1/Nop2p_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031341">
        <property type="entry name" value="Methyltr_RsmF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004573">
        <property type="entry name" value="rRNA_ssu_MeTfrase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17125">
        <property type="entry name" value="Methyltr_RsmF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00563">
        <property type="entry name" value="rsmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 12 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="13">
        <location>
            <begin position="169"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="12">
        <location>
            <begin position="258"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="381"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS86138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="443" mass="49577" checksum="049396F23A76F58F" modified="2012-04-18" version="1">MSQNNAREVAWQILQQVRQQHAYSNLQLNQSLHQADLNDADQRLVTNLVYGVLQHQITLEYWLAPFIKGKKVAAWVRTLLLMTLYQYHYLDRIPDWAATNEAIEIAKRHGNPGIRKFVTGVCHAILRQGVADLNTIKDPIQRLSVVASLPQWLIEKLSDQYGMQVTKAISVAVNQPANQSLRVNTNLTNTEAVQAELETAGFEVKLSPLADNAFIIKSGSVTETTAFKEGRITIQDESAMLAVESMHLKPDDLVLDACAAPGGKTVQIAENLTTGKVVALDIHAHKVKLIEQNAQRMQVADRIQALQLDARKVDERFADETFDKILVDAPCSGFGLLRRKPEIRYDKTWQDSQNLHRIQGVILDSVAPKVKKGGIITYSTCTILATENDGTVQAFLARHPEFELMTTKTARAIKADRHAKTLTILPNEFNSDGFFISNLKRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJV7</accession>
    <name>H4GJV7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_20434</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000050">
        <property type="protein sequence ID" value="EHS86031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122340.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000050.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20434"/>
    <dbReference type="EnsemblBacteria" id="EHS86031">
        <property type="protein sequence ID" value="EHS86031"/>
        <property type="gene ID" value="PS3_20434"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1137"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="242" mass="26624" checksum="55712B3CA7E21C07" modified="2012-04-18" version="1">MTARILLTNLQKSFGSQQVLAGINFDVEPGEIVGLIGPSGAGKSTAIKTMLGMEKADGGEALVLDHHMPNRQILGEIGYMAQSDALYESLTARENMRFFGQMRGLSHQELAPQIEAAAKVVQLTPDLDKRVSGYSGGMKRRLSLAIAFLGNPPLLILDEPTVGIDPALRRQIWTDLKRLRDQGTSILVTTHVMDEAELTDRVALLINSQIIAFDTPQQLKRQYQVDSIEEVFLAAEQPKEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJW9</accession>
    <name>H4GJW9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_9985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS85986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9985"/>
    <dbReference type="EnsemblBacteria" id="EHS85986">
        <property type="protein sequence ID" value="EHS85986"/>
        <property type="gene ID" value="PS3_9985"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1156"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="20"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="310" mass="35305" checksum="C54A886BEF65A979" modified="2012-04-18" version="1">MAGHFCCHDKIEVFRRCDMFSQIKYFIAVVEQHSFTLAAEECHISQSAISQQIKELEATLGVALLDRHGRTFTVTAAGQYFYRHGKDLLVSVDFLIKGTQQFNEPDQYQLNVGYLRDFGTGEFLQAVADFSSQYPQIKINITSGNHESLYRLMQSQKLDLIFSDQRRALSDRAINHFLTASPLMVAASVNQLKDRQNVDVDDLKDLTCLIIQSDPEDTSTIEYYQGALGISSDFQIVGTKEAAEMLMATNQGYVIVNERTQDQFNSAVIQARPLVHHQEKFLQKYFAYWDKDNGGYFVEAFAKLLANQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJZ3</accession>
    <name>H4GJZ3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD(P)-bd_dom domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_22126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS86010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122376.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_22126"/>
    <dbReference type="EnsemblBacteria" id="EHS86010">
        <property type="protein sequence ID" value="EHS86010"/>
        <property type="gene ID" value="PS3_22126"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1203"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="7"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="208" mass="22620" checksum="21B9B108E9FFB5D7" modified="2012-04-18" version="1">MKIFVAGASGRVGHKVVQELVAAGHQVVAGSRNPDLAIATEQIVPVKLDLHADPETIKPLLSEVEAIVFTAGSRGQDLLQTDAYGPVKLMQLAQSLGINRFVMLSALYTLEPSKWSEKLKNYYIAKFFADNYLVNQTDLDYTIIQPGILVDEAGTGNVILGEQGLTSIPIPDVAKVLATVVDQPNTYHQVIQINPGEHSIEQVFTELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK02</accession>
    <name>H4GK02_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Capsular polysaccharide biosynthesis protein CpsC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_10542</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000053">
        <property type="protein sequence ID" value="EHS85874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122385.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000053.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10542"/>
    <dbReference type="EnsemblBacteria" id="EHS85874">
        <property type="protein sequence ID" value="EHS85874"/>
        <property type="gene ID" value="PS3_10542"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1224"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009103">
        <property type="term" value="P:lipopolysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032807">
        <property type="entry name" value="GNVR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003856">
        <property type="entry name" value="LPS_length_determ_N_term"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13807">
        <property type="entry name" value="GNVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02706">
        <property type="entry name" value="Wzz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Wzz" evidence="8">
        <location>
            <begin position="5"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="GNVR" evidence="9">
        <location>
            <begin position="156"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="252" mass="28010" checksum="C84253B4FE9E3A75" modified="2012-04-18" version="1">METSNTIDLRRLVKILRKNLLMLIVWTLGLGIIAAADAEFLVQPKYTASTQILVNNKENKVQAGQAYNNQQADVQLINTYKDIITNQVILQEATKDLANPETSHANGKAYDLSVAELKDALSVSTQTNSQVFTLNAEASNPTEAKAMANTVASVFKKRVKKIMDVNNVTIVSSATTPKKKSFPNVKLFALAGAVVGFVISFGYLLIKELFDTTVRTNEFMTDELNLVNLGQVSHMRYDRKNKRQKSRGNRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK29</accession>
    <name>H4GK29_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Adenylosuccinate lyase</fullName>
            <shortName evidence="11">ASL</shortName>
            <ecNumber evidence="4 11">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PS3_6968</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:23921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = AMP + fumarate</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:16854"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="4 11"/>
    <dbReference type="EMBL" id="AICN01000055">
        <property type="protein sequence ID" value="EHS85845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122412.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000055.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6968"/>
    <dbReference type="EnsemblBacteria" id="EHS85845">
        <property type="protein sequence ID" value="EHS85845"/>
        <property type="gene ID" value="PS3_6968"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1261"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 11" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADSL_C" evidence="12">
        <location>
            <begin position="349"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS85845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="431" mass="49036" checksum="EA25D0DAF7D58E8C" modified="2012-04-18" version="1">MIDRYTRPEMGAIWTDENKYNCWLAVEIAVDEAWAEAGHIPAEDVEKIKQNAKFSVDRINEIEAVTHHDVIAFTRTVSESLGDEKKWVHYGLTSTDVVDTAQGYQLKQANDILRQDLQDFIDILAKRAREFKDTVCMGRTHGIHAEPTTFGLKFARWYAELKRDQERFEHAAKGVEAGKISGAVGTFAEVDPFIQDAVCKKLGLHSQEISTQVLPRDLHAEYIAAMALCATSIENIATEIRALQRTEIHEVEEHFAKGQKGSSAMPHKRNPIGSENMTGMARLLRGHVVTAYENDALWHERDISHSSAERVILPDTTIGLDYMLHRMGNILKNLDVFPETMKRNMDKTLGLIYSGRVLLKLVESGLSREAAYDLIQPYTAKCWAEQIPFRPLLEADPTIQKQLSKEKLDEAFDYHWHLRNVDEIFHRVGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK38</accession>
    <name>H4GK38_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_8311</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000055">
        <property type="protein sequence ID" value="EHS85854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122421.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000055.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8311"/>
    <dbReference type="EnsemblBacteria" id="EHS85854">
        <property type="protein sequence ID" value="EHS85854"/>
        <property type="gene ID" value="PS3_8311"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1272"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="7">
        <location>
            <begin position="128"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="128"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="229" mass="25723" checksum="14D1717DFF590CF9" modified="2012-04-18" version="1">MKILVVDDDREIVQLLEIYIRNEGYEPVTAFDGKEALSKLNTDHEIGLIILDLMMPEMDGMEVIKQVRKDSSMPILVLSAKTADMDKIHGLVSGADDYVTKPFNPLEVMARVNSLLRRSEHEVTNDQPDVLHAGPLEINQDSHEVKTLKGDHIQLTALEFGILYLLASHPNRVFSADDIFERVWKQESVVSAKTVMVHVSHLRDKIEEATGGEKVIETVWGVGYKVSAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK40</accession>
    <name>H4GK40_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_21982</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000056">
        <property type="protein sequence ID" value="EHS85807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122423.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000056.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21982"/>
    <dbReference type="EnsemblBacteria" id="EHS85807">
        <property type="protein sequence ID" value="EHS85807"/>
        <property type="gene ID" value="PS3_21982"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1276"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038766">
        <property type="entry name" value="Membrane_comp_ABC_pdt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30287">
        <property type="entry name" value="PTHR30287"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="620"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="672"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="714"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="280"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="652"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="752" mass="84719" checksum="822C95030CAEB4E0" modified="2012-04-18" version="1">MKYLNRKLWRDVRHNWTQFFSVFLMAFLSILVFVGLQGAWKGLDTSLDHYISDAKLANYWVQATNVTDNDLSKLRDLKGISKVSAGTRIQVKQNSHQLIIDSLDHPVTKLHLVKGTAYSNQRSGVWVNKEYATNHNLKVGQTISMRYQGQTVRLKIRGIVKSASRIYFNGTQEYIAPNYSNYGYAYVSPQTLTNEFHYAGGNNLIEIRGNHHQMRKAVEKIFGARLVAYYNRTTLPDVSSALDRVGQIRNLSYLFSFIFILLAILAMFTTIRRLIEDQMGEIATLKALGFQNRQLSLHYASFGLLVGGAGTVVGALFSPVISWFVLSTQQAMFSIPHWQLSYTYSSLAVIALVILICIGAADWAARGSMRGLPAKFLRGETESRVHHIWLERIRGWRKLSYACRWAIRDVFFNKSRVLMGIVGVAGGMMLMIAGIGMPQSINHLVEQAYGHDFTYSKRLNVNNYQQYQQTNHQNGQWVQLSQAHYSPDDGYDRLLMVISKGSSKGDYVQMKTIDGHKVEDGGVYVTQGFADRANLSKGMTLKIKTVGLSDPIKLKIKGIITSETNQGAYVTSKTWQDAGETFQPTTLLVGKGAKYDKSAISSMITMNSQRQNAERFVNNLMSIFALIIAFAVLLIVIVLYNLGALSFVERSRDYATLSVLGIKRNELRNLTLIENLITTFVGWLIGIPAGIWFLGEYVNTFSTINLVYSPYINWITITVATVFVWVCSLSTTLLISRRIRKLDMVEALKGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK67</accession>
    <name>H4GK67_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative L-threonine 3-dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_13220</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000061">
        <property type="protein sequence ID" value="EHS85628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13220"/>
    <dbReference type="EnsemblBacteria" id="EHS85628">
        <property type="protein sequence ID" value="EHS85628"/>
        <property type="gene ID" value="PS3_13220"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1313"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ADH_N" evidence="4">
        <location>
            <begin position="36"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="ADH_zinc_N" evidence="3">
        <location>
            <begin position="187"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="348" mass="37336" checksum="42CC018B7102BC9F" modified="2012-04-18" version="1">MFVFFITNGGIIVKALVLTGLKQLEVQDYDKPTIKPDEVLVHTAFAGICGTDKALYAGLPGSAEAVPPIVLGHENSGVVAEVGSAVTNVKVGDRVTVDPNIYCGQCKYCRTQRQELCEHLDAVGVTRDGGFEEYFTAPAKVVYPIPDSVSLKAAAVIEPISCAMHGVDLLDLHPYQKAFVLGDGFEGQLFAQILQASGVHEVTLGGRNDEKLANNREHFGVKTLNTVTDEIPEAEYDIVIEAVGTPETQEQALHAAARGAQVLMFGVGNPDDKFSVNTYDVFQKQLTIQGAFINPYTFEDSIALLASGKVDPLPLFSHELELSEVEDFVAGKLGKVSKAIVKVGGEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK68</accession>
    <name>H4GK68_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_3013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000061">
        <property type="protein sequence ID" value="EHS85629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122451.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000061.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3013"/>
    <dbReference type="EnsemblBacteria" id="EHS85629">
        <property type="protein sequence ID" value="EHS85629"/>
        <property type="gene ID" value="PS3_3013"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1315"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="13"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="135" mass="14994" checksum="6F7D953013E7F117" modified="2012-04-18" version="1">MAFNKSLAGYFDDLQHVGLPTDDLKKTVDFYAGLGFKMNGNFDTDDQGNQVVFMTLGHLTLEIWTGDGAVRQTGAINHLSFNTSDADAAYKAAKAAGYQMKENEVQHLDFWDKGIKFFNIAGPNDETLEFCEIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK79</accession>
    <name>H4GK79_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_21473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122462.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21473"/>
    <dbReference type="EnsemblBacteria" id="EHS85531">
        <property type="protein sequence ID" value="EHS85531"/>
        <property type="gene ID" value="PS3_21473"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1337"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032083">
        <property type="entry name" value="DUF4811"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16069">
        <property type="entry name" value="DUF4811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="174" mass="20059" checksum="97F2C34AE983B129" modified="2012-04-18" version="1">MIIIFTIIWALGFYWFTAVKQTMRHHIAYGMICLIFLIANVTAFVLHDNYHLGMTTQSSVSTQQLSSLSPRAKLLAYRSLGNGREKIYVYKTTTKTKIQHTKADTNVTTKVSITSTKPQVKVTTIKYRYANKLSQWMFGILGTDNETKSINYHFYVNTDWLVLSTDQLQQMTQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK81</accession>
    <name>H4GK81_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_15255</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Responsible for synthesis of pseudouridine from uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a uridine in RNA = a pseudouridine in RNA</text>
            <dbReference type="Rhea" id="RHEA:48348"/>
            <dbReference type="Rhea" id="RHEA-COMP:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:12069"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122464.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15255"/>
    <dbReference type="EnsemblBacteria" id="EHS85533">
        <property type="protein sequence ID" value="EHS85533"/>
        <property type="gene ID" value="PS3_15255"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1340"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140098">
        <property type="term" value="F:catalytic activity, acting on RNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034470">
        <property type="term" value="P:ncRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluC/D_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PseudoU_synth_2" evidence="4">
        <location>
            <begin position="86"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="292" mass="32990" checksum="CD015EB7BD16C186" modified="2012-04-18" version="1">MENTWVYRGTEPIKIKQYLHSLGMGHRLFNDIKNGQGEFLVDHRSVRPTTKILPNQPLTIKVQPEPIDPTVQVSAADLSVVYEDDNWLVVNKPAGVSSVPGPTQPTDTILNRVTGYLRQGQSPDQRPHLVTRLDRYTSGLMLIAKHNVATSMISPQVQTHQMLKEYLAVVQGRLPEPHQLITSPIARVAGQAQRVVDPDGQKAETEYWVEETKGDYSLVRLQLHSGRTHQIRVHLNSIGYPIVGDALYGGDCSIVDHQLLQAFRLAFVDPFSEEQRSFELPMDERFNQIWSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK87</accession>
    <name>H4GK87_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Citrate lyase holo-[acyl-carrier protein] synthase</fullName>
            <ecNumber evidence="2">2.7.7.61</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_6387</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2'-(5''-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA + apo-[citrate lyase ACP] = diphosphate + holo-[citrate lyase ACP]</text>
            <dbReference type="Rhea" id="RHEA:16333"/>
            <dbReference type="Rhea" id="RHEA-COMP:10157"/>
            <dbReference type="Rhea" id="RHEA-COMP:10158"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61378"/>
            <dbReference type="ChEBI" id="CHEBI:82683"/>
            <dbReference type="EC" id="2.7.7.61"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.61" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122470.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6387"/>
    <dbReference type="EnsemblBacteria" id="EHS85539">
        <property type="protein sequence ID" value="EHS85539"/>
        <property type="gene ID" value="PS3_6387"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1346"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050519">
        <property type="term" value="F:holo-citrate lyase synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051191">
        <property type="term" value="P:prosthetic group biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005551">
        <property type="entry name" value="CitX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03802">
        <property type="entry name" value="CitX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03124">
        <property type="entry name" value="citrate_citX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="180" mass="20370" checksum="CC18885D15AE6310" modified="2012-04-18" version="1">MEIFNQSPAVTIEQVLANRDRRVKLQASLLTDEYPTVIAIKLNIPGPVKNGPTIRQYFEEVNRQFETGLKAQGWPYKLSLMDLESVTGPESFYQLQADPRSVKKLTRQFEESSADHRLLDMDVIYQDGAQPRSISRADLNLPARTCLICGRLAKDCGRSRRHTVAELQQKVAELIEGALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKE1</accession>
    <name>H4GKE1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_17723</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000064">
        <property type="protein sequence ID" value="EHS85433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS85433">
        <property type="protein sequence ID" value="EHS85433"/>
        <property type="gene ID" value="PS3_17723"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1436"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS85433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="128" mass="14601" checksum="45B72ED0FCE39F09" modified="2012-04-18" version="1">MIDKERCSMYNQITITPTELVVSPAGLDRLWALKGPIRVPWRHVTGIFRDQGAFKEHKGLRLPGTAIPGLYYAGSFFYDGQRRFYNVRRNTPIVVITLINEGYDRLILSVENPTTVIERVTGHLLNKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKF1</accession>
    <name>H4GKF1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Pyridine nucleotide-disulfide oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PS3_20541</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="8">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000064">
        <property type="protein sequence ID" value="EHS85443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122533.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000064.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20541"/>
    <dbReference type="EnsemblBacteria" id="EHS85443">
        <property type="protein sequence ID" value="EHS85443"/>
        <property type="gene ID" value="PS3_20541"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1460"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016668">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 10" id="KW-0274">FAD</keyword>
    <keyword evidence="2 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 10" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="12">
        <location>
            <begin position="6"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="11">
        <location>
            <begin position="333"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="431"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="166"/>
            <end position="173"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="9">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS85443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="449" mass="49950" checksum="55F2D6269199D33E" modified="2012-04-18" version="1">MDKFKNIIIGFGKAGKTLAKYLAQHDETVLLIERSKQMYGGTCINVGCLPSKNLIFNGQRGVDFTTAVNKRGEMTRQLRNKNYHMVADEPLATVWDGAARFIDNYVLAVVMSDGTTKKVRGERIFINTGAVPNWPSIPGLEFSQRIFTSKEAMELKKQPKRLAIIGGGYIGLEFAGMFNSFGTHVTIFDQHTRLLEREDPDIATEVVTDLTNAGIEIKLETQLTQVKDNGEKVTLYYQQGDQTNTAEFDAVLVAVGRWPNVSSLGLENTDIALTNRGAIQVDDHLRTTVQNIWALGDVNGGPMFTYVSLDDFRIIIDQLFGKGNRSTADRMVIPTASFLNPPLANVGLNERQAKSAGYDLQTFKLSVKAIPKARVLEDQRGLYKVIVDQKTHLILGATLYATEAHETINLIALAMKAKLPYERLRDMIYTHPTMSEALNDLFKTPVVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKH2</accession>
    <name>H4GKH2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal kinase</fullName>
            <ecNumber evidence="1">2.7.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_13392</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.35" evidence="1"/>
    <dbReference type="EMBL" id="AICN01000067">
        <property type="protein sequence ID" value="EHS85386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122554.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000067.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13392"/>
    <dbReference type="EnsemblBacteria" id="EHS85386">
        <property type="protein sequence ID" value="EHS85386"/>
        <property type="gene ID" value="PS3_13392"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1502"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008478">
        <property type="term" value="F:pyridoxal kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009443">
        <property type="term" value="P:pyridoxal 5'-phosphate salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004625">
        <property type="entry name" value="PyrdxlKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534">
        <property type="entry name" value="PTHR10534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phos_pyr_kin" evidence="2">
        <location>
            <begin position="21"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="281" mass="30626" checksum="5206DE12D02C4F6F" modified="2012-04-18" version="1">MESTIIIEDFSALGQISMVASLSILQTFGCATAALPTSLLSTQTEGFGQPVQLATSAWFKATISHWQDQNVKISTALIGYLGNQAIISDVIDALTKFRPSLVLVDPAMADQGELYPGLDSDYVDAIRQLCHKADLITPNVTELALLTDREVPKWIDLAWVQNCLNKLESMGILASVVVSGVRMADQIGSVIAANNQLEFIASPYFFGHFYGTGDTFAPLLLGFLRRGVSLKEAVQKSTKLMEIAVEETSHLTADDRKYGLRLAKLLGKLSTEVANGRQKDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKH3</accession>
    <name>H4GKH3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_6921</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000067">
        <property type="protein sequence ID" value="EHS85387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6921"/>
    <dbReference type="EnsemblBacteria" id="EHS85387">
        <property type="protein sequence ID" value="EHS85387"/>
        <property type="gene ID" value="PS3_6921"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1509"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039369">
        <property type="entry name" value="LacA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024688">
        <property type="entry name" value="Mac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43017">
        <property type="entry name" value="PTHR43017"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12464">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01266">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Mac" evidence="4">
        <location>
            <begin position="4"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Mac" evidence="4">
        <location>
            <begin position="192"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="389" mass="43081" checksum="18F23BE093738C24" modified="2012-04-18" version="1">MDVFDRMQNGYLYRPEDPNLGISNTEQLKKQRLFNQTADHTILKRMFAAFGDGSQLRPPLFSVWGGQFVEVGRDVKIGPGLTLVDDAPIIIGDRVQIGASVTISVAGHPVDPTLRRRRLAFNLAVHLDDDCVIGDQVIILPGVTIGKGAIIEAGSIVTKDIPAHVVAAGNPCRVIQQVQVEKVEPKMAKSEFEKMRDGSLYLPEDPAILAEQQIHMKLQYQYNNLEPGDPKQAAMLKKMFADCGEECYIEPPLHANWGGFHTHLGDRVYANFNLTLVDDADIYIGDDTMIGPNVNISTAGHPIWPAYRLKKYQHNQQVHIGKNCWLGANVTVLPGVTIGDNTVVGAGSVVTKDLPANVVAVGNPCRVLREINDHDRQYYYKDRRVDLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKH7</accession>
    <name>H4GKH7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Aminopyrimidine aminohydrolase</fullName>
            <ecNumber evidence="6 8">3.5.99.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_16189</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes an amino-pyrimidine hydrolysis reaction at the C5' of the pyrimidine moiety of thiamine compounds, a reaction that is part of a thiamine salvage pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>4-amino-5-aminomethyl-2-methylpyrimidine + H2O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:31799"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16892"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:63416"/>
            <dbReference type="EC" id="3.5.99.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>H2O + thiamine = 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17509"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16892"/>
            <dbReference type="ChEBI" id="CHEBI:17957"/>
            <dbReference type="ChEBI" id="CHEBI:18385"/>
            <dbReference type="EC" id="3.5.99.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the TenA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.99.2" evidence="6 8"/>
    <dbReference type="EMBL" id="AICN01000067">
        <property type="protein sequence ID" value="EHS85391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122559.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000067.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16189"/>
    <dbReference type="EnsemblBacteria" id="EHS85391">
        <property type="protein sequence ID" value="EHS85391"/>
        <property type="gene ID" value="PS3_16189"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1518"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050334">
        <property type="term" value="F:thiaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004305">
        <property type="entry name" value="Thiaminase-2/PQQC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027574">
        <property type="entry name" value="Thiaminase_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03070">
        <property type="entry name" value="TENA_THI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="SSF48613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04306">
        <property type="entry name" value="salvage_TenA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0784">Thiamine biosynthesis</keyword>
    <feature type="domain" description="TENA_THI-4" evidence="9">
        <location>
            <begin position="11"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="219" mass="25109" checksum="B5A009DBCA4DAB2A" modified="2012-04-18" version="1">MTFTDQIQEAAMPLWQRSFVHPFIQELTQGTLPLSTFRYYLIQDYFYLKNFCDLHGEIAAQSQVEAERNALLAGERTLNHGEVAIRQGMFKTLKIEKAEVNQLGIAPTCYNYVNHMRAALAAGGPEVGIAALLPCYWLYHAVGQHLQAHGSPVALYQQWIDNYIDDDYAQAVDRMLDLVNQGAQQVDSTTRDQMAESFIRSSAYELQFWEMAYQKEQWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKK1</accession>
    <name>H4GKK1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aldehyde-alcohol dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_6961</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the iron-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122583.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6961"/>
    <dbReference type="EnsemblBacteria" id="EHS85281">
        <property type="protein sequence ID" value="EHS85281"/>
        <property type="gene ID" value="PS3_6961"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1547"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008774">
        <property type="term" value="F:acetaldehyde dehydrogenase (acetylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006066">
        <property type="term" value="P:alcohol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015976">
        <property type="term" value="P:carbon utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08178">
        <property type="entry name" value="AAD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034789">
        <property type="entry name" value="AAD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018211">
        <property type="entry name" value="ADH_Fe_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012079">
        <property type="entry name" value="Bifunc_Ald-ADH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="PTHR11496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000111">
        <property type="entry name" value="ALDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00913">
        <property type="entry name" value="ADH_IRON_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="3">
        <location>
            <begin position="14"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Fe-ADH" evidence="4">
        <location>
            <begin position="466"/>
            <end position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR000111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="869" mass="94975" checksum="A053962838485E95" modified="2012-04-18" version="1">MVEKAKPKMTKERAEEIVDGLVKKAHEALDIMSDFDQEKVDYITHQMVEAGQDKHMELAVEAYNETGRGVIEDKAIKNMFATEEIWHHIRKHKTVGIIEDDKANELIKVAEPLGVIAGITPVTNPTSTTLFKAIIAMKTRNPIIFSFHPQALQSSRHAAKILRDAAIAAGAPEGAIQWIDEPSRETSTALINHPGVASTLATGGPGMVTAAYSTGKPALGVGPGNGPVYIEKTANLKRAVNDIILSKTFDNGMICATENSAVIDADIYKEVTDMFKANRMYFVPKKDLDKLRKAMFNPETGSVLGPIAGKSAMQIAEMAGIEVPADTQVLAAEITGVGKDEDLLSGEKLSPVLSIYKAKDQEEAFDICARLLDYGGLGHTAAIQTSDMELAKQYGIQMKASRVIVNSPSGLSGIGNLYNRMTPSLTLGTGSWGANSISHNVTDYDLINVKTIAKRRNNMQWIKLPRVYFERDAVRYLKDMPDINRVFIVTGPSIQAHGYVDVVTDQLKLRQNEVPYMVFSDVENDPSTETVKKGVALMNSFKPDTIIALGGGSPLDAAKGMWMFYENQDASFFGAKQKFINIRKRAYRFEKPTKAQMVAIPTTSGTGSEVTPFAVITDSETHVKYPLTDYALTPDVAIVDPQFVDTVPKGNIAASGLDVLAHAMESYVSNMASDYTRPLSLQAIKLVFENLTKSYNGDAKAREEMHNASTLAGMAFANAFLGINHSIAHKLGGAFGLTHGIAIAITMPHVIKFNSTLPKKFTTWPKYEYFRADEDYATIAKTIGLSGKNNEELIDKLCLAVRDLAHSVGVKLTLKDWGVSKKDFDAKVDDLAVLAYEDQCTTANPQEPLISDLKQIMINEYDGTGVEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKK3</accession>
    <name>H4GKK3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13 14">UvrABC system protein B</fullName>
            <shortName evidence="13">Protein UvrB</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Excinuclease ABC subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">uvrB</name>
        <name evidence="19" type="ORF">PS3_14263</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 13">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11 13 14">Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The beta-hairpin motif is involved in DNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13 14">Belongs to the UvrB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000070">
        <property type="protein sequence ID" value="EHS85230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122585.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000070.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14263"/>
    <dbReference type="EnsemblBacteria" id="EHS85230">
        <property type="protein sequence ID" value="EHS85230"/>
        <property type="gene ID" value="PS3_14263"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1554"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00204">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004807">
        <property type="entry name" value="UvrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041471">
        <property type="entry name" value="UvrB_inter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024759">
        <property type="entry name" value="UvrB_YAD/RRR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24029">
        <property type="entry name" value="PTHR24029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12344">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17757">
        <property type="entry name" value="UvrB_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="SSF46600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00631">
        <property type="entry name" value="uvrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 13 14" id="KW-0228">DNA excision</keyword>
    <keyword evidence="9 13 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 13 14" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 14" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="31"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="435"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="16">
        <location>
            <begin position="632"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="628"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Beta-hairpin" evidence="13">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="44"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EHS85230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="670" mass="76498" checksum="9A865FE52A8D0A65" modified="2012-04-18" version="1">MIYRQADRPFELVSKFQPTGDQPEAIQQLTDGIQAGEKAQILLGATGTGKTFTISNVIANVNKPTLILSHNKTLAGQLYGEMKEFFPNNAVEYFVSYYDYYQPEAYVPSSDTYIEKDASVNDEIDQLRHSATSSLIERNDVIVVASVSSIFGLGSPAEYQNHVVSLRVGQEISRNRLLRELVLIQYDRNDIDFQRGRFRVRGDVVEIFPASREERALRIEFFGDEIDRIREVDALTGEVLGDREHASIFPATHFMTSEETMEVALPEIEADMHKQVAKFTKEGKLLEAQRIQQRTTYDIEMMREMGYTNGIENYSRYMDRRKPGEPPYTLLDFFPKDFLLIVDESHQTMPQVRGMYNGDKARKEMLIDYGFRLPSALDNRPLKLAEFEQHVHQVIYMSATPGPYEEEQTQDVVQQIIRPTGLLDPTIEVRPVMGQIDDLIGEINTRVEKNERVLVTTLTKKMSEDLTDYLKDAGLKVKYLHSDIKTLERTEIIRDLRLGKFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRNERSLIQTIGRAARNAHGAVIMYADTITDSMQAAITETKRRRTIQEAYNQAHGITPQTIIKPIREAISAVKPVEDPEEDQSAEFTEKDFMQLSKADQQKMLDDLNEQMRSAAKRLDFEQAAILRDTVMELTAKMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKM4</accession>
    <name>H4GKM4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease R</fullName>
            <shortName evidence="5">RNase R</shortName>
            <ecNumber evidence="5">3.1.13.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rnr</name>
        <name evidence="8" type="ORF">PS3_11027</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.13.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RNR ribonuclease family. RNase R subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.1" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122606.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11027"/>
    <dbReference type="EnsemblBacteria" id="EHS85193">
        <property type="protein sequence ID" value="EHS85193"/>
        <property type="gene ID" value="PS3_11027"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1581"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008859">
        <property type="term" value="F:exoribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01895">
        <property type="entry name" value="RNase_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040476">
        <property type="entry name" value="CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013223">
        <property type="entry name" value="RNase_B_OB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022966">
        <property type="entry name" value="RNase_II/R_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004476">
        <property type="entry name" value="RNase_II/RNase_R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011805">
        <property type="entry name" value="RNase_R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17876">
        <property type="entry name" value="CSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08206">
        <property type="entry name" value="OB_RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00358">
        <property type="entry name" value="3_prime_RNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02063">
        <property type="entry name" value="RNase_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="631"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="719"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="720"/>
            <end position="747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="778" mass="88543" checksum="68864B7E1DE2AACA" modified="2012-04-18" version="1">MAEIKEQILQYLQAHTNQGFAAEILGSNLGMNSADEFTSLIQMLAQLEREGKVEVTDLGEFKAVVQPQQVTGIFHGNPKGFGFVAYDENQADVYVNADHTMHALNGDEVVVKILRPGNGKEQGPEGQVTEIITHSYTRVVGEFKHLTMGNFIGEIILKDKKLANYKFYVTDQGINPQDNDVVTGTIATYPNDDAPQVMTGPAMGVVGNKDEPGVDILSVVYAHDVPHEFPEEALAQADLIPETVQPEEKLGRPDLTDQPLVTIDSIESKDLDDAVVAWRLDNGNYHLGVHIADVSHYVRPGTPLDEEAYRRGTSVYLTDRVIPMLPKKLSNGICSLNPNEERLAMSCEMEIDPQGNVVNHKIFPSVIRSHARMTYKAVNLILESHDEKTRAEYQDLVPMFETMADLHRILLKHRQHRGAIDFEAPEAKIIVDDFGHPIDIEVRDRGLSERMIESFMLAANETVAKHYDQMHVPFLYRIHEHPDSERIRNFAEFLSVFGIQMKGDLNNVQPKMLQNVLKDVAGTPEEQMVQVMMLRSMQQAKYSDEERGHFGLGAQYYTHFTSPIRRYPDTTVHRLIKWYYDHGKGEEAKAKYRDRLGEIAEHTSVTERRGIDTERDVDSLKKAEYMEDHVGETFDAVVSSVMKFGLFVSLPNTVEGLIHISVMKDDFYEYVEKHMALVGRSHHHIFQIGQPVKVKLLRVDKDLREVDFELVNPREAPVTKLRVPKADDRRGGRRGQRNSHSSRNRDNGNRNNRQINRGQARHRPHDDGPQKRDRRRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKN1</accession>
    <name>H4GKN1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">1,4-dihydroxy-2-naphthoate octaprenyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_7147</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Quinol/quinone metabolism; menaquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122613.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7147"/>
    <dbReference type="EnsemblBacteria" id="EHS84951">
        <property type="protein sequence ID" value="EHS84951"/>
        <property type="gene ID" value="PS3_7147"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1591"/>
    <dbReference type="UniPathway" id="UPA00079"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009234">
        <property type="term" value="P:menaquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd13962">
        <property type="entry name" value="PT_UbiA_UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026046">
        <property type="entry name" value="UBIAD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13929">
        <property type="entry name" value="PTHR13929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005355">
        <property type="entry name" value="UBIAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0474">Menaquinone biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="303" mass="34168" checksum="FCD5D45730B6B96C" modified="2012-04-18" version="1">MTLKVFLELVEIKAKTASILPCLLGFCFAWYHYQRFEWLPALVFFVAMVLFNMAVDMLDNYHDFVHAVDTEDYQQNTNIIGRESLSAQQILKALIVLVVIAGALGLWLVYLVGIPVLWMGIFCFAIGFLYSSGPFPMSGLPVGEFFSGFTMGVMITLISVYVNTFSVFTWSWSNLLSIWAVALANELWISNLLLTNNICDAKEDEDNHRHTIIHFIGIRAGLWAFAIKNAVALMAIAITVLIGLQPWTVLATWLLIPFIIQQTRLLFYKQVKKETFSTAVKILFFGSLTQVITFALGILLEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKQ5</accession>
    <name>H4GKQ5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyruvate dehydrogenase complex E1 component beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_15994</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122637.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15994"/>
    <dbReference type="EnsemblBacteria" id="EHS84975">
        <property type="protein sequence ID" value="EHS84975"/>
        <property type="gene ID" value="PS3_15994"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1628"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transket_pyr" evidence="1">
        <location>
            <begin position="5"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="343" mass="37247" checksum="B72B722E7A1C6A66" modified="2012-04-18" version="1">MAREILFMKAINEALDQAMAKDETVVLLGEDIAGGAEVKHLEEQNEDAWGGVMGVTKGLMQKYGRERVIDTPISEHGYMSSSVGMAVTGLRPVPELMFNDFIGFCFDALLGQGSKKRYMFGGKAKVPMTVRTMHGAGASAAAQHSGTYYGLFGSIPGLKVVVPATPYDAKGLLLSSIEEDNIVIFSEDKTLYGMKGEVPEEYYTIPIGKAAVRREGSDLTIVTIGKMLYVAYEVADQLAKDGISTEVIDLRTVAPWDQETVLNSVKKTGRLIIIDESNPHNNTATDIAAVVTDQAFDYLDGPVKRVTAPNTPVPFATNLEQLYIPNAQRVIETASELIDDLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKR5</accession>
    <name>H4GKR5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Uridylate kinase</fullName>
            <shortName evidence="7">UK</shortName>
            <ecNumber evidence="7">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Uridine monophosphate kinase</fullName>
            <shortName evidence="7">UMP kinase</shortName>
            <shortName evidence="7">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pyrH</name>
        <name evidence="9" type="ORF">PS3_18549</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the reversible phosphorylation of UMP to UDP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + UMP = ADP + UDP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="7">Allosterically activated by GTP. Inhibited by UTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the UMP kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122647.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18549"/>
    <dbReference type="EnsemblBacteria" id="EHS84985">
        <property type="protein sequence ID" value="EHS84985"/>
        <property type="gene ID" value="PS3_18549"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1638"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04254">
        <property type="entry name" value="AAK_UMPK-PyrH-Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_B">
        <property type="entry name" value="PyrH_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011817">
        <property type="entry name" value="Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015963">
        <property type="entry name" value="Uridylate_kinase_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005650">
        <property type="entry name" value="Uridylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02075">
        <property type="entry name" value="pyrH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="8">
        <location>
            <begin position="8"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Involved in allosteric activation by GTP" evidence="7">
        <location>
            <begin position="21"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="13"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="136"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS84985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="241" mass="26198" checksum="B80BE4829591B09C" modified="2012-04-18" version="1">MTENIKYNRVVMKLSGEALAGEQGFGINPPEIQTVAKELKEVYELGVEIAIVVGGGNMWRGITGEKLGMERAQADYIGMLGTIMNALSLQDALESLDVPTRVQTSIEMRQIAEPYIRRKAIRHLQKGRVVIFAGGTGSPYFSTDTTAALRAAEVNADAILMAKNGVDGVYSADPKTNDDAVKYERLTHMDIIQKKLHVMDSTASTLSMDNDIPLVVFNLNTPGNILKVVKGENIGTTIEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKS5</accession>
    <name>H4GKS5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_15366</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122657.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15366"/>
    <dbReference type="EnsemblBacteria" id="EHS84995">
        <property type="protein sequence ID" value="EHS84995"/>
        <property type="gene ID" value="PS3_15366"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1651"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018580">
        <property type="entry name" value="Uncharacterised_YfhO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38454">
        <property type="entry name" value="PTHR38454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09586">
        <property type="entry name" value="YfhO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="935"/>
            <end position="957"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="963" mass="109106" checksum="E5C61A91AF22BA51" modified="2012-04-18" version="1">MKFSFAPRTDKRQLYLGYTILFVFIAACLYCPYLIAGKTLIWDTDAFNQHLPLLAQYRQLVWHWLLHPFSSLNFWSFNWGLGTDTFQVFSYYTIGDVFAYIALLFPAAKITLAYQLTIVIRMYCVGLAFVYFAQHLPFCKLVIWGGAATYLVNSYLLYANVAQPFFTTTFIIFPLIIIQIERVLQGRSAWPLTLVFIWMLINNYYLAFILGVGAIIYLGLRVVTFYRHQLDYGRTFWKLVFATVTSLLVSAVMLIPEIIAVRSSTRIGSVFANGLTTYPQYYYVLLAKQLINGDDWDFMYWSALGMTSVIVVALVYVYRHAKQYPLITISLGASLVMMLIPAVGALFNGGMSPSNRWTLLIYLPLATSIMILLEHLTKIDRTTLKWMVGISLVYLMTVVATYMFKNDDDLFVPVIFLLLSLLIVGAAYYRAVTHPLNWMIGLILLNVGFNAVYAALPFNGSFANQMLTKGEYQQIAKQRYGGLDKSLSRSLTYRINTISNNQTTGQEKTYNDLTSGLASISSYFSLQNQYLGLFSQRLGNSQYETNIPLQQVDDRTVLNNFLGVKYIFAQTTTDDDTKVPSGYFVDQTTEPTVNYDQGQSSDATKTSDLKTTQTVRYTTKNAFPLLYWQNTYISPATYRHLSATAKERALASGVVVNSSAAKKWTTGMKKANLSGNVQVIATQLWNNRLQKMNGQSVTVTDPDAKYYLRLQKQTSAHQNELHLDIKSIHYQPFSMKKTLQLMADHLRETTLEPGAMINQVFTNYQNWRSLFLKGYPDMSYLLTASNKYGDETLEQPAQSKTSFFKVVTSGVLNLGAYGKHLSSQVTLTTNKLGKYRISYQLVAEQLGTKYRQEVRQIQAHGLKNLKISQNQISGNLTTSQVGILTSSIPYSSGWTATDNGKTVRLVRTNNAFIGLRLKTGHHHIVLRYHVPGLKAGMHVSLIGLLWLIISAIFTLNWRRKTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKU0</accession>
    <name>H4GKU0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
            <ecNumber evidence="3 8">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pdhA</name>
        <name evidence="10" type="ORF">PS3_15367</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>(R)-N(6)-lipoyl-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + H(+) + pyruvate = (R)-N(6)-(S(8)-acetyldihydrolipoyl)-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10480"/>
            <dbReference type="Rhea" id="RHEA-COMP:10481"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2 8">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="3 8"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122672.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15367"/>
    <dbReference type="EnsemblBacteria" id="EHS85010">
        <property type="protein sequence ID" value="EHS85010"/>
        <property type="gene ID" value="PS3_15367"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1668"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017596">
        <property type="entry name" value="PdhA/BkdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF41">
        <property type="entry name" value="PTHR11516:SF41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03181">
        <property type="entry name" value="PDH_E1_alph_x"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="E1_dh" evidence="9">
        <location>
            <begin position="48"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="364" mass="40327" checksum="40A495291EE0772A" modified="2012-04-18" version="1">MKKLDFESLKYYTGDEKPFQVLSQDGEVVDKDLMPDLTDEQLVDLFKEMIWSRVVGDRSTKLNRQGRLGFIAPTQGQEASEMASNYAMEKDDFLAGAYRDIPQLVKHGLPLAQAFMWSKGHYNGTKFPEELKALPPQIIIGAQYAQTAGIALGLKKKGTKNVAYSYTGDGGTSQGDFYEGINFAGAFHAPAVFIVQNNGYGISVPRAKQTAAPTLAQKGVAAGLPCFQVDGMDALAVYATVKQARDYAAEGNGPVLIETLTYRYGAHTLSGDDPSRYRSSDEEAEWHAKDPIERMRNFLTKKGLWTEEQENAYVDEVNAEVDAAVKELESQPAQTVSEMLKFEFVDTPATIQKQIKKYEEKEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKW0</accession>
    <name>H4GKW0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_6517</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Responsible for synthesis of pseudouridine from uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a uridine in RNA = a pseudouridine in RNA</text>
            <dbReference type="Rhea" id="RHEA:48348"/>
            <dbReference type="Rhea" id="RHEA-COMP:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:12069"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122692.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6517"/>
    <dbReference type="EnsemblBacteria" id="EHS85030">
        <property type="protein sequence ID" value="EHS85030"/>
        <property type="gene ID" value="PS3_6517"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1694"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluC/D_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="4">
        <location>
            <begin position="14"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="304" mass="34090" checksum="0C031FB962898B0A" modified="2012-04-18" version="1">MAPQHIVADSTMQGRLDKVLAQHLTDISRSQIKNWIDQGRVTVNHQQVKAKYNLLVGDQIEITPPQPKTIDLTPEAMALDIVYEDDDVIVVNKPQGMVVHPSAGHPNHTLVNGLLYHSPLSTINGEFRPGIVHRIDKDTSGLLMVAKNDHAHQSLAAQLKAKTNEREYLALVHGVIKEDEGKIDAPIGRSLKDRKQQAVVANGKSAVTHFKVLKRYQHYTLISCHLETGRTHQIRVHMKYINHPLAGDPLYGPRKTLPGNGQYLHARTLGFVHPTTGKLMRFSAPLPDWFEKMIQFLDRTDAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKW4</accession>
    <name>H4GKW4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N6-adenine-specific DNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_6953</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122696.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6953"/>
    <dbReference type="EnsemblBacteria" id="EHS85034">
        <property type="protein sequence ID" value="EHS85034"/>
        <property type="gene ID" value="PS3_6953"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1698"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034470">
        <property type="term" value="P:ncRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RNA_methylase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01261">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="THUMP" evidence="3">
        <location>
            <begin position="44"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="378" mass="42769" checksum="C38080A8AE8EDBF5" modified="2012-04-18" version="1">MTEFKLLATAAAGIEALVNRELKDLGYQTQTENGRVRFQGDIKAIARTNLWLRTADRVKIIVGEFDAKTFDDLFEQTKALAWEELLPIDANFPVSGRSSHHSQLHNVPSIQAITKKAIVERLSMVYHRRTRLTESGSKYPIEVAIDKDHVILTLDTTGDSLFKRGYRQAKGAAPMKENLAAALVMLAHWFPDNPFVDPVCGSGTIPIEAALIGHNIAPGINRHFMMEQWLNLTPAELLDEVRDEADAQAHYDVELDITGYDIDQSMVAIAQQNARAAGLSQDITFKQLALKDWRTEKLNGVIVANPPYGERLSDQANVRELYRQMGQLYRPMTTWSKYILTADLEFEKFYGAPATKKRKLYNGALRTDLFQYWGKKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKY9</accession>
    <name>H4GKY9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_6944</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122721.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6944"/>
    <dbReference type="EnsemblBacteria" id="EHS84755">
        <property type="protein sequence ID" value="EHS84755"/>
        <property type="gene ID" value="PS3_6944"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1732"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="27"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS84755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="233" mass="24873" checksum="1321913F8A2E44E7" modified="2012-04-18" version="1">MINLLNSWFPNVSSLGWTGDSGWGTAIGQTIYMTVWPAIFGGIIGLVFGVALVITKPGGILENAFWFGLCDKVVSVFRAIPFIILLAFIAPFTQLLVGTQIGMTAALVPLSVGVAPFYARQIQVALESVNPGKVEAAQALGATNWDIILNVYLKEARSEIARVSTVTLISLVSLTAMAGAIGAGGLGNTAISYGYNRFNNDVTFVATLLVLLFVLIIQLIGDWLAKSLNHQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL02</accession>
    <name>H4GL02_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methionine synthase II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_5733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122734.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5733"/>
    <dbReference type="EnsemblBacteria" id="EHS84768">
        <property type="protein sequence ID" value="EHS84768"/>
        <property type="gene ID" value="PS3_5733"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1750"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Meth_synt_2" evidence="1">
        <location>
            <begin position="10"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="371" mass="42230" checksum="CD4002E96A780624" modified="2012-04-18" version="1">MTKQFLHADIVGSFLRPEDLKQAHADFAAGKLDAAGLKQVQNKDIQELVQAEVAAGLKAVTDGEFSRSWWHLDFLWGLNGTERYDYNESYKFKGSKTRTDNVRLTGKIAYNPEHPFFEAFKYLQSVTPAGVVPKQTIPSPTMLFRDNRSDLALNFYDNWEDYLADLAQAYHQTIQHFYDLGCRYIQLDDTTWAFLIAKFLETKDDFQEQAKYRKLAEDAVTVINGALKDLPEDLTVTTHVCRGNFKSTYLFSGGYDVVAKYLAQLNYDGFFLEYDTDRAGSFAPLKQIWNNRDNVRVVLGLVTSKFPELESEDLLKERIQAATEFVPLKNLALSTQCGFASTEEGNSLTSAEQWNKIKLVVKVAQDVWPVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL04</accession>
    <name>H4GL04_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_9580</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nlpA lipoprotein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122736.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9580"/>
    <dbReference type="EnsemblBacteria" id="EHS84770">
        <property type="protein sequence ID" value="EHS84770"/>
        <property type="gene ID" value="PS3_9580"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1752"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004872">
        <property type="entry name" value="Lipoprotein_NlpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30429">
        <property type="entry name" value="PTHR30429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03180">
        <property type="entry name" value="Lipoprotein_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002854">
        <property type="entry name" value="MetQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS84770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="283" mass="31241" checksum="CC8C16F9D1C4A26C" modified="2012-04-18" version="1">MSRQVKRWLGCLIVLIVILGGLGYFGYRRYQSSRVTTVKLGLVGTDSEPVWNNVKKRLTKQNIKLQYVVFNDYVQPDKALKDGEIDMHACLTKYYFESYNKSQNAHLVSIGNTVISPLGLYSKKYSKLSDLPDGATIALPNEPTTIGRALNMLQAAGLIKVKAGSGIKPSLNDITDNPKNLKFKEVDPAQTPRALTSVDAAVINGNYAVSAKIKPGGKQALYMEKINQAAKPYVNIIAVQAKNKNNKVYQAVVKAYQTEATEKAIQKTYHGAQQAAWPIYGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL05</accession>
    <name>H4GL05_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent helicase/nuclease subunit A</fullName>
            <ecNumber evidence="10">3.1.-.-</ecNumber>
            <ecNumber evidence="10">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">ATP-dependent helicase/nuclease AddA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">addA</name>
        <name evidence="14" type="ORF">PS3_15029</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domain is required for chi fragment generation; this subunit has the helicase and 3' -&gt; 5' nuclease activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer of AddA and AddB/RexB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the helicase family. AddA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="10"/>
    <dbReference type="EC" id="3.6.4.12" evidence="10"/>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122737.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15029"/>
    <dbReference type="EnsemblBacteria" id="EHS84771">
        <property type="protein sequence ID" value="EHS84771"/>
        <property type="gene ID" value="PS3_15029"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1753"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01451">
        <property type="entry name" value="AddA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014152">
        <property type="entry name" value="DNA_helicase_suAddA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070:SF48">
        <property type="entry name" value="PTHR11070:SF48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02785">
        <property type="entry name" value="addA_Gpos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6 10 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="12">
        <location>
            <begin position="4"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="13">
        <location>
            <begin position="503"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="25"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS84771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="1274" mass="145354" checksum="8F36114A1F0257BE" modified="2012-04-18" version="1">MSSFKPSAEQQPAIEHRGHDILVSASAGSGKTAVLVERVIQLLEKDRLNIDQLLMVTFTKEAAKNMRERIRKRLMASPDQHMKEQISRLAVANISTIHSFCEQLIKRYYYTIDLDPNYRLVTDAEQNLLMDQVWHDLVNDWLTGDQKATYLRVADNFAQGVKGEGLAGVVRKMDQEANAQPDPNGWLTHLIDNYELDDQQAITDTHFYQAVVKPIAQAQLLELLNQWQNLSQVAENDKLRERLAEDLSKLEQLFINGELVDDWDLLVAATEKKSFGINPKKTKDSTDIELAQLNQRNALKSQLDEIHKTYFSWSSQRYHALLPNAKAILKELVSFSQQFRQRYQRAKQQRHLLDFSDLEHYAYAILTGRAVLTNELLATHQATATQIRRELQFQYQEIMVDEFQDTNRLQYDLLQQLHDPKRNHLFMVGDMKQSIYRFRQADPTLFLENYQRFRQADDPNEALDLSDNYRSMTNITEFTNVVFKQLMNQGVGEMDYDQTAELRAKAQWQLADGQVAPARQTEMLVYRTNHNPAIVGEDRLTGEVQMVATRIRQLLDDEKETIFDQDTQTMRRIKPNDIVILSRTKAANSQIVEQFGQLNIPVTVHGVENYFKATEIRIVMSLLRIIDNPYQDVPLVAVMRSPLITLPTDEAVIGFTEPEMAIIKLNHPDGAYYEAIQQFAQDFPEDDFPGNHVRDGIDYYIVAQKVNRLLTLLNHLRACARQRSLAELIWEIYDQTNYLDYVGGMPGGPQRQANLHALYERASAYEQSSFKGLYQFIRFIEQMQRKDDDLGVAPADLANDSINVMTIHGSKGLQFPIVFLIDMNHQFRKDSEGVVIEPRTGIGMSLVENVHDQAADLPPVEVKYELPQQTVIKEQLKRQNRAEEMRLLYVALTRAEQRLIITASVNDAPTNKLGNLKRDWNFCSQAVQSKSLVLNDALRLKANSMFDWLGLTLFRHPQFPSILDELDKPVFDEGSLQLGDVDFTVQLVDQALVRQWQANLPDVRKAGQTQLSLFGEDQPPLSKTQSQLMQQILRFEYPHQAATETTAYQAVSTIREAFRIQDPDDVAMGRLTIDADQVRGEGAYLKDTMAVPEFMQTNQAVTPTVLGTATHLLFERLDLSHGEVTFSQVSALRDQLVDQGLIPSPAVGDQLDLTAIVAFYQTDLGQRILAHPDQVQREVAFSMLLPGAKLFNNLTDQDGPILVHGIIDGYLVTDEGVELFDYKTDQITRTKLPQLRQRYQGQLALYASALASMLDCPLAKIKSDLYSVTCQSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL32</accession>
    <name>H4GL32_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Single-stranded-DNA-specific exonuclease RecJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PS3_10566</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RecJ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122764.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10566"/>
    <dbReference type="EnsemblBacteria" id="EHS84662">
        <property type="protein sequence ID" value="EHS84662"/>
        <property type="gene ID" value="PS3_10566"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1786"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004610">
        <property type="entry name" value="RecJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018779">
        <property type="entry name" value="RecJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041122">
        <property type="entry name" value="RecJ_OB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17768">
        <property type="entry name" value="RecJ_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10141">
        <property type="entry name" value="ssDNA-exonuc_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00644">
        <property type="entry name" value="recJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHH" evidence="7">
        <location>
            <begin position="83"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="8">
        <location>
            <begin position="315"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="RecJ_OB" evidence="10">
        <location>
            <begin position="457"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="ssDNA-exonuc_C" evidence="9">
        <location>
            <begin position="570"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="308"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS84662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="774" mass="85764" checksum="40B44E830FC93B0B" modified="2012-04-18" version="1">MRKAKYQWHQLSSDQTDLVQTIQEQCQIPSFLAQLLVERGCDSLEKAQHFLKPQLQDLHGPEELHGIEAAVEKIEAAISQDWQITVYGDYDADGLTSTALMYETLRDIGAKVDYYIPNRFTDGYGPNQAVYQRLIEQGTQLILTVDNGISGNEAIDYANRQGIEVVITDHHSLPAQLPAATAIVHPQYPGDEYPYADLSGVGVAFKMAWALLGEFPYELLDLVAIGELADLVSVQGENRSLITFGLQQLRSGMRTGLHELIKLTNADEEHLTDQDVGFQIAPRLNALGRMGDAATGVELLTTFDEEVAKELAQQVDQANQERQALVKAINLAAEKMAQAAPNRDRQTLLLVGKDWHQGVLGIVASHIVQVTGKPTIIASTNEDGTLAKGSGRGVAGFDLFQALDDHRDLMTSFGGHTMACGLSFDVDQTTDLAQALEAAAQKQAFDASQQPSLEIAGQLRPSQVTAEMYEQIHRLAPFGPGNEEPIIEITNAEISQAILIGEERQHVKLTLTNQPPINVLAFNQPQLASEMTVGSTANLVGCLSLNRWKGQTTAQLMFKDAETTGIMIEDHRTNQLAPQMFQQAAVYLVFNDQLRANIYGHTNGPVISPQTISELGMQEQRLIIVDCPTDIDQFAEVINQVGMVAVVDLWLYTSHQPVDQVPDRQHFIRLYQAMQSQPVIDSRTQLPALGQYLRVTNDQLIFMIQVFIEAGFVIIDNGLLKSVKNVKSVDLTKTQRYQRRQQWLKVQNTLLTVPTTSLRQQISAWLGDKLEENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL46</accession>
    <name>H4GL46_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">30S ribosomal protein S20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpsT</name>
        <name evidence="8" type="ORF">PS3_4959</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds directly to 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the bacterial ribosomal protein bS20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122778.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4959"/>
    <dbReference type="EnsemblBacteria" id="EHS84676">
        <property type="protein sequence ID" value="EHS84676"/>
        <property type="gene ID" value="PS3_4959"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1801"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00500">
        <property type="entry name" value="Ribosomal_S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002583">
        <property type="entry name" value="Ribosomal_S20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036510">
        <property type="entry name" value="Ribosomal_S20_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33398">
        <property type="entry name" value="PTHR33398"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01649">
        <property type="entry name" value="Ribosomal_S20p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46992">
        <property type="entry name" value="SSF46992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00029">
        <property type="entry name" value="S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="84" mass="9199" checksum="4598067666557D1E" modified="2012-04-18" version="1">MPIIKSAIERVRVNEKAAARNASQMSAMRTAVKKFDKAKLAGADNLEELYKNAVSAIDRAHSKGLIKANKAARDKSRLAARYAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL47</accession>
    <name>H4GL47_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA polymerase III, delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_15385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122779.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15385"/>
    <dbReference type="EnsemblBacteria" id="EHS84677">
        <property type="protein sequence ID" value="EHS84677"/>
        <property type="gene ID" value="PS3_15385"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1802"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010372">
        <property type="entry name" value="DNA_pol3_delta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005790">
        <property type="entry name" value="DNA_polIII_delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06144">
        <property type="entry name" value="DNA_pol3_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="SSF48019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01128">
        <property type="entry name" value="holA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_pol3_delta" evidence="1">
        <location>
            <begin position="19"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="341" mass="38177" checksum="52F8A331B233280D" modified="2012-04-18" version="1">MDIPTLKKQLKGASPAMVYTVVGTQMELQAETRQAFLDLIPAEERVMNVASYDLEATPLDEALADAMAAPFFGERRLVFLNKPTFLTGSTGRGSLKQDPDLLKEYLNHPEPTTTLVIMAPYEKLDGRKGVVKSLKKVAVEVSAAPLDESQARHIVEARFQKDGFILQNGAMDELVRRTNADYGQMMSHVQQLETYAYPGKDITIAAVQGLVPQSLDENVFDLVQAVLDQHQVMALDDYQQLLAAQQQPLQINAVLVSQFRLLLQVKILSQRGLSQGTITQQLKVHPYRVQLAQRTAQKYDLATLCQAYQGLIQIERDLKTTSRSPELLFQLFMLKFNKAVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL55</accession>
    <name>H4GL55_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal subunit assembly factor BipA</fullName>
            <ecNumber evidence="4">3.6.5.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">GTP-binding protein BipA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bipA</name>
        <name evidence="6" type="ORF">PS3_7300</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">A 50S ribosomal subunit assembly protein with GTPase activity, required for 50S subunit assembly at low temperatures, may also play a role in translation. Binds GTP and analogs. Binds the 70S ribosome between the 30S and 50S subunits, in a similar position as ribosome-bound EF-G; it contacts a number of ribosomal proteins, both rRNAs and the A-site tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Binds to ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. BipA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122787.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7300"/>
    <dbReference type="EnsemblBacteria" id="EHS84685">
        <property type="protein sequence ID" value="EHS84685"/>
        <property type="gene ID" value="PS3_7300"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1811"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03710">
        <property type="entry name" value="BipA_TypA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00849">
        <property type="entry name" value="BipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035651">
        <property type="entry name" value="BipA_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042116">
        <property type="entry name" value="TypA/BipA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006298">
        <property type="entry name" value="TypA_GTP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF8">
        <property type="entry name" value="PTHR42908:SF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01394">
        <property type="entry name" value="TypA_BipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Tr-type G" evidence="5">
        <location>
            <begin position="5"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="17"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="130"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS84685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="614" mass="68772" checksum="5C5A4C208630C5E1" modified="2012-04-18" version="1">MKLRDDIRNIAIIAHVDHGKTTLVNEMLKQSNTLPEHFDIQDRAMDTNDLEKERGITILSKNTAVKYGDYQINILDTPGHADFGGEVERVMKMVDGVLLVVDAFEGTMPQTRFVLQKALEQHLTPIVVINKVDRDGARPEEVVDEVLDLFIELGADEDQLDFPVIYASAMNGTSSYDSDISTQEHTMKPLFDTIVEKIPAPVDNSDEPLQFQVSILDYNDFVGRIGIGRVVRGQIHVGDQVTLMKLDGSTKNFRVTKLFGFVGLKRIEIDSAKAGDLIAVSGMEDINVGETVVDPEHPEALTPLRIDPPTLQMTFRTNDSPFAGREGKFVTSRQLQNRLESELETDVSLRVEDTDDPGAWVVSGRGELHLSILIETLRREGFELQVSRPEVIYREIDGVMSEPYEEVQIDTPDEYTGSIIDSLSKRKGEMQNMEPSNNGQTRLIFEVPSRGLIGYSTEFMSLTRGYGIMSHVFDQYKPVIKNWNPGRQKGTLVSMNAGKATTYAMMGIESRGTLLIDPGTEVYEGMIIGENSRENDITVNITKGKNLTNVRAAGSDDMARIKTPTHLSLEESLEFLNEDEYCEITPENVRLRKQILETNAREKAAKRRKAASRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL65</accession>
    <name>H4GL65_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_4118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122797.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4118"/>
    <dbReference type="EnsemblBacteria" id="EHS84695">
        <property type="protein sequence ID" value="EHS84695"/>
        <property type="gene ID" value="PS3_4118"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1825"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1820.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014965">
        <property type="entry name" value="Amino_acid_metab_prot_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035942">
        <property type="entry name" value="Lp2179-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08866">
        <property type="entry name" value="DUF1831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160800">
        <property type="entry name" value="SSF160800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS84695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="113" mass="13311" checksum="68037D1E49ABDF72" modified="2012-04-18" version="1">MQFQASQQITGDSTKYQISEHIKQYPLIDLGFEKTRQGNYKYEGSLDRQNPFKPVAKLRIVINQDLTGFKMETVNANGLQKINIYQMPRAEEFKTQLQFIMDEMVEREIFKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL66</accession>
    <name>H4GL66_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Adenosylhomocysteine nucleosidase</fullName>
            <ecNumber evidence="2">3.2.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_14276</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.9" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122798.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14276"/>
    <dbReference type="EnsemblBacteria" id="EHS84696">
        <property type="protein sequence ID" value="EHS84696"/>
        <property type="gene ID" value="PS3_14276"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1826"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00871"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008782">
        <property type="term" value="F:adenosylhomocysteine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008930">
        <property type="term" value="F:methylthioadenosine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009164">
        <property type="term" value="P:nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010049">
        <property type="entry name" value="MTA_SAH_Nsdase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01704">
        <property type="entry name" value="MTA/SAH-Nsdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="6">
        <location>
            <begin position="3"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS84696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="232" mass="24108" checksum="E471877F0AE613EA" modified="2012-04-18" version="1">MIYGIICAMPEEIKELTAQLEHPVTTTIGDKDYLSGQISGQEVVLVESGIGKVEAAITAEHLITDLKADVVINSGSAGGIGEGLAVGDVVVSTATAYHDVDVTAFGYENGQLPGQPARFTADQSLVAALVKAGAETGLTVRTGLIVSGDQFIASQAQIAVIKQKFPDVLSSEMEGAAVGQVANDHQVPYAVVRAMSDNGDEDAGVSFDEFIIEAGQRSAKMLLQLFANLAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL93</accession>
    <name>H4GL93_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative phage protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_16430</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000079">
        <property type="protein sequence ID" value="EHS84622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16430"/>
    <dbReference type="EnsemblBacteria" id="EHS84622">
        <property type="protein sequence ID" value="EHS84622"/>
        <property type="gene ID" value="PS3_16430"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1861"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025280">
        <property type="entry name" value="DUF4041"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018306">
        <property type="entry name" value="Phage_T5_Orf172_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13250">
        <property type="entry name" value="DUF4041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10544">
        <property type="entry name" value="T5orf172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00974">
        <property type="entry name" value="T5orf172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T5orf172" evidence="3">
        <location>
            <begin position="274"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS84622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="387" mass="45157" checksum="7DFBE86F7EA13363" modified="2012-04-18" version="1">MEQEDLISDVEDLKDELNHLKMEIEEKSKVLADVGDQIYFEQFGLYKPHYSFANSSSYKAQLDQTRATQKNMIRENIAATIFRPMMLDNSKSKGEAMQKKNIKQLLRAFNGECEAAINRVTKANIETIEKKISNSFNQLNKLNGPNGVRISTAYFDEKINESHIALEYAMKKDEEKELLREEREREREERQLQQELSREKKKYEKDEDHFIKAQQELTEKISQTNDSTEIDSLKSELAELQAQLDEIRVKKDKLTDRAENPTAGYVYIISNIGSFGKDVFKIGVTRRLEPMDRINELGSASVPFKFDVHALIFSNDAFKLESELHQHFNKQRLNQVNNRKEYFNITIDDVKDVLKNYQNLTFDFHEIPDAIEYRESCKIKQSISQAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLA4</accession>
    <name>H4GLA4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="6">2.7.8.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Phosphatidylglycerophosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PS3_18151</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">This protein catalyzes the committed step to the synthesis of the acidic phospholipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate = 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12593"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60110"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.5" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122836.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18151"/>
    <dbReference type="EnsemblBacteria" id="EHS84603">
        <property type="protein sequence ID" value="EHS84603"/>
        <property type="gene ID" value="PS3_18151"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1880"/>
    <dbReference type="UniPathway" id="UPA00084">
        <property type="reaction ID" value="UER00503"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01005"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006655">
        <property type="term" value="P:phosphatidylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004570">
        <property type="entry name" value="Phosphatidylglycerol_P_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000847">
        <property type="entry name" value="Phos_ph_gly_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00560">
        <property type="entry name" value="pgsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="15" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EHS84603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="194" mass="21612" checksum="5765E62136824CDA" modified="2012-04-18" version="1">MNLPNKLTVLRLILIPFLLLLMVLPLNWGTVSWWGASIPVAELIAAIIFIGASITDNLDGQIARRQHLVTNFGKFADPLADKLLVMTAFVLLVEQHVLPGWAVSIIVWRELAITGLRLLVVEQNGQVMAAQMPGKIKTTTQLIAIIFLLLHNVGFALINVPFGQIMFYICLFFTVYSGIDYIYQARSVFADGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLA5</accession>
    <name>H4GLA5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">M16C subfamily protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_12945</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122837.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12945"/>
    <dbReference type="MEROPS" id="M16.A20"/>
    <dbReference type="EnsemblBacteria" id="EHS84604">
        <property type="protein sequence ID" value="EHS84604"/>
        <property type="gene ID" value="PS3_12945"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1882"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M16" evidence="2">
        <location>
            <begin position="59"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16_C" evidence="3">
        <location>
            <begin position="178"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="334"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS84604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="429" mass="48193" checksum="18533E38E85AA68F" modified="2012-04-18" version="1">MGAKMADPVVRKVLANGLTVQLLPMANFHKTYAILTTNFGSVDRKYKLSPNDEWKTIPDGIAHFLEHKLFEKENYDAFELFGEHGADANAFTGFTQTSYLFSTTSQLKENLEVLLDFVQAPYFSAASVAKEQGIIGEEIKMYADNPGNRLYMGMMENLYPHDPMHIDIAGTVESIAQITPETLYEVYRAFYQPSNMTLSVVGALDVEETFSWIEDNQAAKEFISAPKPIRYPIISDSSGADVIKSRTISMAVERPKVMVGLRGLSSWSKGRDRLKAKSAIELGLEMLFDDTTDNYLQLYNRGILDDSFSFSFEIERGFHLASFATETDQVQAFIDEMKKVLENAEQLLVADQDQFEATKRASLGRLIFNLDSPEAIANRFEGALFDQATIYDEIAVLEEITFEDVLSATLNFIKPMCLTAMVINPLHND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLC0</accession>
    <name>H4GLC0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UspA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_21234</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122852.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21234"/>
    <dbReference type="EnsemblBacteria" id="EHS84619">
        <property type="protein sequence ID" value="EHS84619"/>
        <property type="gene ID" value="PS3_21234"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1899"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="5"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS84619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="159" mass="17334" checksum="9920A2E0D87ADF2C" modified="2012-04-18" version="1">MSQEYKHILVPVDGSKEAELAFKKSVAVAKRNGDDTELQLLHVVDTRAFQNISSFDSSMVEQVTDTAKKTLDDYVAYAKEQGVANVSYTIEYGAPKTLIAKEVPAKMHADLIMIGATGLNAVERVVIGSVTEYVTRTAVCDVLVVRTGLDNEPVENAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHV9</accession>
    <name>H4GHV9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">4-hydroxy-tetrahydrodipicolinate reductase</fullName>
            <shortName evidence="9">HTPA reductase</shortName>
            <ecNumber evidence="9">1.17.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dapB</name>
        <name evidence="13" type="ORF">PS3_9399</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NAD(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:35323"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:35331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the DapB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.8" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121643.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9399"/>
    <dbReference type="EnsemblBacteria" id="EHS87515">
        <property type="protein sequence ID" value="EHS87515"/>
        <property type="gene ID" value="PS3_9399"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.84"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00018"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016726">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00102">
        <property type="entry name" value="DapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022663">
        <property type="entry name" value="DapB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022664">
        <property type="entry name" value="DapB_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023940">
        <property type="entry name" value="DHDPR_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836">
        <property type="entry name" value="PTHR20836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05173">
        <property type="entry name" value="DapB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000161">
        <property type="entry name" value="DHPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00036">
        <property type="entry name" value="dapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01298">
        <property type="entry name" value="DAPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0520">NAD</keyword>
    <keyword evidence="4 9" id="KW-0521">NADP</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DapB_N" evidence="11">
        <location>
            <begin position="4"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="DapB_C" evidence="12">
        <location>
            <begin position="127"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="(S)-2,3,4,5-tetrahydrodipicolinate binding" evidence="9">
        <location>
            <begin position="160"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="8"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="94"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="120"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>(S)-2,3,4,5-tetrahydrodipicolinate</name>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS87515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="259" mass="27914" checksum="C37A1AB49D197DE2" modified="2012-04-18" version="1">MKTVLIAGFNGSMGQQAVKLVNSMEGFEITAGLAHHLPDSDPTSYGLPATALLFTSLDEIPTGIADIWLDFTVPTAVYANVKYALTHGYRPVVGTTGLTDDQEVELKKLTQDNHLGGLIAPNFGMSAVLLMKFAQEAAKYFPDVEIIEMHHEDKKDAPSGTALNTAKLISKNRPAHQTAPDETESLAGSRGGDYEGIKLHAVRLPGYVAHEQVLFGGAGEALTIRQDSFDRKSFMSGVKVALEQVHTLDQLVVGLEQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHX0</accession>
    <name>H4GHX0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="8">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glycyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="8">GlyRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">glyS</name>
        <name evidence="9" type="ORF">PS3_3769</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121654.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3769"/>
    <dbReference type="EnsemblBacteria" id="EHS87526">
        <property type="protein sequence ID" value="EHS87526"/>
        <property type="gene ID" value="PS3_3769"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.96"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00255">
        <property type="entry name" value="Gly_tRNA_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015944">
        <property type="entry name" value="Gly-tRNA-synth_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006194">
        <property type="entry name" value="Gly-tRNA-synth_heterodimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30075">
        <property type="entry name" value="PTHR30075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02092">
        <property type="entry name" value="tRNA_synt_2f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01045">
        <property type="entry name" value="TRNASYNTHGB"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00211">
        <property type="entry name" value="glyS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50861">
        <property type="entry name" value="AA_TRNA_LIGASE_II_GLYAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="689" mass="77962" checksum="5531EE08F4E4D6EA" modified="2012-04-18" version="1">MAHTYLLEIGLEEMPAHVVTPSMNQLQSRVENYLKDQRIDYQTIKAFSTPRRLALKIEGLSDKQPDVDELVKGPAKKIALDDQGNWSKAAIGFTKGQGVSVEDIEFQEVKGTEYVFVNKHIPGESVATVLAGLDQVITAMTFPTLMKWGEYKLDYIRPIHWLVSLLDDAVVPFKILNVTADRLTKGHRFLGHDLELTNASDYEEALLNDFVIADPKARKAKIKAQIQAIADEHQWQLDWDEELLEEVNNLVEWPTSFAGKFDQQYLVLPEVVLITSMKDNQRFFCVRNQAGQLQPAFIAVRNGNTEHLDNVIKGNERVLVPRLEDAKFFYEEDQKITIDQYVERLSRVSLHDQISSMAEKMARTGVIAMALAEQLNFSDQEKADLKRAATIYKFDLTTQMVGEFAELQGIMGEIYAKLAGENETVAAAIREHYMPISAEGALPATKVGALLAIADKLDSIYSFFAVGMVPSGSNDPYALRRQAYGIVRIINDQQWHLPVLDLQTQLPALFTAADLHPKFDIAENNDQVRQFFVDRLKQFFSLQSIRHDIVDAVCASEQPDLLAVIDAAQVLVKHQSDDNLKNQMEAITRVIRLAKKEALTTDEVIVKPELFKNPSEQRLFNQFNEFDDQSSLNDQFEQLMALEPVISQYFEENMIMDPDEAIRQNRLAQLTLVAQAASQFGDLDLLIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHX8</accession>
    <name>H4GHX8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RmuC family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_21510</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA recombination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RmuC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21510"/>
    <dbReference type="EnsemblBacteria" id="EHS87534">
        <property type="protein sequence ID" value="EHS87534"/>
        <property type="gene ID" value="PS3_21510"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.104"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003798">
        <property type="entry name" value="DNA_recombination_RmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30563">
        <property type="entry name" value="PTHR30563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02646">
        <property type="entry name" value="RmuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="25"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="287"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="333" mass="37763" checksum="75FEDFD5086B1AB8" modified="2012-04-18" version="1">MQQLKQGPQGNLDVSNQLYQNRQENEQLRQTIDQKLTQIQQNNDQQLERMRQTVEEKLESTIQSRLKQSMANVNQQLESVNKSLGEMQSLSQDVASLNKTLSGTKTRGIMGEVQLGNVIEDILTSAQYEREVPTIAGSSNRVEFAIKLPGEHQQTVWLPIDSKFPLDSYERIQTALDNSDREQVDKERKALAQSIRKFAQDINSKYIQPPQTTDFGIMFLPTENLYSEVVRNAQLVEELRKQENVIVTGPSTIAAILNALSVGFKTLTIEQNAQKISQTLGKFKAEFESYNDILTKAQKQLNDASKNLDRLSSTRTKAMDRVLRDVEALDDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI13</accession>
    <name>H4GI13_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_18537</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LemA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000007">
        <property type="protein sequence ID" value="EHS87464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18537"/>
    <dbReference type="EnsemblBacteria" id="EHS87464">
        <property type="protein sequence ID" value="EHS87464"/>
        <property type="gene ID" value="PS3_18537"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.147"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023353">
        <property type="entry name" value="LemA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007156">
        <property type="entry name" value="MamQ_LemA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34478">
        <property type="entry name" value="PTHR34478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04011">
        <property type="entry name" value="LemA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140478">
        <property type="entry name" value="SSF140478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="168" mass="18904" checksum="AEE92AC17E43AF6C" modified="2012-04-18" version="1">MVAYNGLVRLRMNGQESWSQIDVQLQRRNDLIPNLVETVKGYGKYEAGTLEKVTSLRQQVASVSSDDHQAKMAAADQLSGALNSLYAVAENYPDLKANEEYSRLMEELSNTENKISYSRQLYNSTVSTLNARIQSFPSNIVAKIHHFTPMEFLQVPESVKEAPKVSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI14</accession>
    <name>H4GI14_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Chloride channel protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_3018</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000007">
        <property type="protein sequence ID" value="EHS87465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121698.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000007.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3018"/>
    <dbReference type="EnsemblBacteria" id="EHS87465">
        <property type="protein sequence ID" value="EHS87465"/>
        <property type="gene ID" value="PS3_3018"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.150"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="Cl-channel_volt-gated"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="SSF81340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="302"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="338"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="394"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="420" mass="44805" checksum="7782A32D14284251" modified="2012-04-18" version="1">MNETKTISRNLPMIIATLVIGTLAGTSSMLLSLFLDQVERFFLGFNENFQQPVAINALPIRRLGSVLVGGIIAALIWYWLQKRYSPVKIQDAIENGAPMPFWYTWVHVLTQIFFVGTGGSIGRELAPREAGAMLAHQWTKLSRRFPRMNLSPEDRRLLIASAAGAGFAGVYIAPITGMMFALELLYKKISARAIAVSLTSSVIATLIGSLAKGWDPYYHVTNLNFSAKSIPWVLVLGLLMGIGGTWFKAGIKKAGQRRFKDQRLLVTLPVVALLTGIIAMGFPQIMGNGRGIAQLAIDTTSISTGTIAIMVAALLAKAIVTLMTIAGGGYGGTLTPSIAVGAAAGIVVGTLWMQFIGGDVLLSQCAIVGAAALLTASQQAPLMSLFMITEVCHLNYSALMPLGLAVAVSIAVSKLMQGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI16</accession>
    <name>H4GI16_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_10550</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000008">
        <property type="protein sequence ID" value="EHS87449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121700.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000008.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10550"/>
    <dbReference type="EnsemblBacteria" id="EHS87449">
        <property type="protein sequence ID" value="EHS87449"/>
        <property type="gene ID" value="PS3_10550"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.154"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="412"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="322"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="472" mass="53618" checksum="4BD8739ABE8A2373" modified="2012-04-18" version="1">MRVIKNYIYNAGYQILLMFTPLITTPYISRVLGAHYTGINEYTNSWVTFFYLIGQMGVILYGNREIAYHRNDDYKRSKTFWEIELLQACTISVSLIAYLMAVFVFSSTFKEYFLLQTLWIIATAIDVSWFFMGMEDFKKTVVRNTIVKIVTIALIFLVVKSSDDLWKYIILLGGAQLAGNLTLWPYLRKLVHWVPLRDLHPFQHFYPAFLLFIPTITTQVYLVVNRLMLGHMVTPADLGNFTYADRLVKLILAVATATGSVMIPNVAQKFANHDILGVRESLYKSFDFVTAISVPMALGLTAIAVKFAPWFLGNQYLQTGTIMMIEAPIVVLIAWSTVTGNQYLMPIDRVKEYTASVTAGAVVNVIANLFLIYWYKANGAALATVISEFTVTAIQLWCIRTTISRRRLFKSLWKYFVCGALMYLAVHAVNLWLPMNILTLAIQIIIGVIVYVASVWLIKAPVVAMAKELLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI20</accession>
    <name>H4GI20_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine--tRNA ligase</fullName>
            <ecNumber evidence="7">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA synthetase</fullName>
            <shortName evidence="7">SerRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA(Ser/Sec) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">serS</name>
        <name evidence="10" type="ORF">PS3_6504</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + L-serine + tRNA(Sec) = AMP + diphosphate + H(+) + L-seryl-tRNA(Sec)</text>
            <dbReference type="Rhea" id="RHEA:42580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:10128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + L-serine + tRNA(Ser) = AMP + diphosphate + H(+) + L-seryl-tRNA(Ser)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer. The tRNA molecule binds across the dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000011">
        <property type="protein sequence ID" value="EHS87430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121704.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000011.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6504"/>
    <dbReference type="EnsemblBacteria" id="EHS87430">
        <property type="protein sequence ID" value="EHS87430"/>
        <property type="gene ID" value="PS3_6504"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.159"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00176">
        <property type="entry name" value="Ser_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43697">
        <property type="entry name" value="PTHR43697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="9">
        <location>
            <begin position="173"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Serine binding" evidence="7">
        <location>
            <begin position="230"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="261"/>
            <end position="263"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="348"/>
            <end position="351"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="432" mass="49078" checksum="0DDFD5D397101B34" modified="2012-04-18" version="1">MLDLKKIRQEPDWYKEKLATRGVEAAEIDSILALDVKRRELLQETEQLKAQRNEASKKIGEAKRNQESADDAIAEMQAVAEKIKGLDEQVNAVDEQLHNELSCLPNIPHEGVPVSLTEDGAKELRVVGQPRQFDFEPKHHWDIGEQLGILDFDRAAKVSGARFVYYVGLGAQLERAVYNFMLDEHMQEGYTEVLPPYIVNAQSMYGTGQFPKFKEDVYQVNGENMTLIPTAEVPLTNYYRGEVIPEEDLPIRVTALSPSFRSEAGAAGRDTRGLIRMHQFNKVEMVKYTKPEQSWEELEMMTANAENILKKLNLPYHVITLTTGDMSFTASETHDLELWMPAQKKYREVSSCSNCLDFQARRMHTQYRDENGKLQYVHTLNGSGLAVGRTVAAILENYQNEDGSVDVPEVLVPYFHGITKITKENAVPFKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI39</accession>
    <name>H4GI39_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA dimethylallyltransferase</fullName>
            <ecNumber evidence="6">2.5.1.75</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Dimethylallyl diphosphate:tRNA dimethylallyltransferase</fullName>
            <shortName evidence="6">DMAPP:tRNA dimethylallyltransferase</shortName>
            <shortName evidence="6">DMATase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Isopentenyl-diphosphate:tRNA isopentenyltransferase</fullName>
            <shortName evidence="6">IPP transferase</shortName>
            <shortName evidence="6">IPPT</shortName>
            <shortName evidence="6">IPTase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">miaA</name>
        <name evidence="10" type="ORF">PS3_3795</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 6 8">Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6 7">
            <text>adenosine(37) in tRNA + dimethylallyl diphosphate = diphosphate + N(6)-dimethylallyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:26482"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="EC" id="2.5.1.75"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6 9">Belongs to the IPP transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.75" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121723.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3795"/>
    <dbReference type="EnsemblBacteria" id="EHS87378">
        <property type="protein sequence ID" value="EHS87378"/>
        <property type="gene ID" value="PS3_3795"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.181"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="PTHR11088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00174">
        <property type="entry name" value="miaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6 7" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with substrate tRNA" evidence="6">
        <location>
            <begin position="34"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with substrate tRNA" evidence="6">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate tRNA" evidence="6">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="307" mass="34792" checksum="284CA1FBE99264EE" modified="2012-04-18" version="1">MVKVVAIVGPTAVGKTALSIQLAQRLNGEIISGDSMQVYRHLNIGTAKVTPTEMAGIPHYLIDIKDVTERFSVAEFKDLAQKKIDEISQRQHLPIIVGGTGFYLKALMENLALGDDHFDDQSRIIRQRWQDRLQSEGSVAIWQALKRVDPQAAHQIPVQNSRRVIRALEVVERTGNLFSAQRTKQAKDEFLVIGLTTERSTLYERINHRVDLMMETGLLDEVKWLQDQGGSELPAAKGIGYREFFSYLAGDDDLASAIERVKLDSRHYAKRQLTWFRNQVATNWYDLVSGENSTNEIEESIVAWLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI56</accession>
    <name>H4GI56_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycine/betaine ABC transporter ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_15790</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15790"/>
    <dbReference type="EnsemblBacteria" id="EHS87395">
        <property type="protein sequence ID" value="EHS87395"/>
        <property type="gene ID" value="PS3_15790"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.204"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="241" mass="26754" checksum="DB678EDBA8BD36BF" modified="2012-04-18" version="1">MSKLLTINDVTKVYGQKTALGNANLTINQNDFVALVGMSGGGKTTLLRMIAGLEQPSTGKIEQSGQEINGINRQSRVMFQDDRLLPWMTVRDNLTFGNHQDDKVRQADELLAAVELADFGDYYPSRLSGGQKQRIALARALMDNPPLLLLDEPLGALDALTRLKMQDLIMDLTQSRQLTTVLVTHDVHEATRMANRIVVVKNSHIAQVFENPYRGSGDDKKAHQAVLAERILDEIYAEEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI60</accession>
    <name>H4GI60_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_20229</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121744.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20229"/>
    <dbReference type="EnsemblBacteria" id="EHS87399">
        <property type="protein sequence ID" value="EHS87399"/>
        <property type="gene ID" value="PS3_20229"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.213"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003772">
        <property type="entry name" value="YceD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02620">
        <property type="entry name" value="YceD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="182" mass="20942" checksum="0C26C0446B8D8991" modified="2012-04-18" version="1">MKWSLEDLSRYHDEPLHLATTFDLNAMMTKRFPEQILAVAPIKVDGYWSFDKGDATIGMHVDTTVTTPSSRSLEPVELQLSFDITESYTNEHSHFDRYDDDEVVFYLEDDELIDLDRILSENIVEQIPLKVLTAAEKAGEALPKGKDWSVITEDQLDQQQHQQVDPRFAKLKNLFPDQDNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI75</accession>
    <name>H4GI75_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Formamidopyrimidine-DNA glycosylase</fullName>
            <shortName evidence="15">Fapy-DNA glycosylase</shortName>
            <ecNumber evidence="15">3.2.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">DNA-(apurinic or apyrimidinic site) lyase MutM</fullName>
            <shortName evidence="15">AP lyase MutM</shortName>
            <ecNumber evidence="15">4.2.99.18</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">mutM</name>
        <name evidence="15" type="synonym">fpg</name>
        <name evidence="19" type="ORF">PS3_15805</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.</text>
            <dbReference type="EC" id="3.2.2.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 15">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the FPG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.23" evidence="15"/>
    <dbReference type="EC" id="4.2.99.18" evidence="15"/>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121759.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15805"/>
    <dbReference type="EnsemblBacteria" id="EHS87414">
        <property type="protein sequence ID" value="EHS87414"/>
        <property type="gene ID" value="PS3_15805"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.229"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008534">
        <property type="term" value="F:oxidized purine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00103">
        <property type="entry name" value="Fapy_DNA_glycosyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015886">
        <property type="entry name" value="DNA_glyclase/AP_lyase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015887">
        <property type="entry name" value="DNA_glyclase_Znf_dom_DNA_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020629">
        <property type="entry name" value="Formamido-pyr_DNA_Glyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012319">
        <property type="entry name" value="FPG_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035937">
        <property type="entry name" value="MutM-like_N-ter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000214">
        <property type="entry name" value="Znf_DNA_glyclase/AP_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010663">
        <property type="entry name" value="Znf_FPG/IleRS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01149">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06831">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06827">
        <property type="entry name" value="zf-FPG_IleRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00898">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01232">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81624">
        <property type="entry name" value="SSF81624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00577">
        <property type="entry name" value="fpg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51068">
        <property type="entry name" value="FPG_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01242">
        <property type="entry name" value="ZF_FPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51066">
        <property type="entry name" value="ZF_FPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13 15" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FPG_CAT" evidence="18">
        <location>
            <begin position="2"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="FPG-type" evidence="17">
        <location>
            <begin position="239"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DNA" evidence="15">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="3"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for beta-elimination activity" evidence="15">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for delta-elimination activity" evidence="15">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>DNA</name>
            <dbReference type="ChEBI" id="CHEBI:16991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>DNA</name>
            <dbReference type="ChEBI" id="CHEBI:16991"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EHS87414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="279" mass="31565" checksum="7361DEEFCD95ED93" modified="2012-04-18" version="1">MPELPEVETVRRGLMRIAAGRQINGIDVLYGKTITNDVEEFRQALIGQTITDIDRRGKYLLFRFTNQLTMVSHLRMEGSYFNQPTGGEVDKHTHVIFHFTDQTDLCYRDTRKFGRMTLVKTGNEYEVGGLKTIGPEPTEHDFKLDYFIDILKHSRGKIKPFLLNQSHVAGLGNIYCDEVLWMAKINPEQPANSLSDTQASILRKSIISEIKRATKLHGTTVHTFKNAFGNAGEFQNELQAYGHAGEPCPRCGTTMIKIKVAQRGTTYCPQCQPLRGQND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI77</accession>
    <name>H4GI77_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">UDP-N-acetylmuramate--L-alanine ligase</fullName>
            <ecNumber evidence="4 12">6.3.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">UDP-N-acetylmuramoyl-L-alanine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">murC</name>
        <name evidence="16" type="ORF">PS3_13440</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP + L-alanine + UDP-N-acetyl-alpha-D-muramate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine</text>
            <dbReference type="Rhea" id="RHEA:23372"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:70757"/>
            <dbReference type="ChEBI" id="CHEBI:83898"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the MurCDEF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.8" evidence="4 12"/>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121761.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13440"/>
    <dbReference type="EnsemblBacteria" id="EHS87416">
        <property type="protein sequence ID" value="EHS87416"/>
        <property type="gene ID" value="PS3_13440"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.232"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008763">
        <property type="term" value="F:UDP-N-acetylmuramate-L-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00046">
        <property type="entry name" value="MurC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005758">
        <property type="entry name" value="UDP-N-AcMur_Ala_ligase_MurC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01082">
        <property type="entry name" value="murC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6 12" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 12" id="KW-0133">Cell shape</keyword>
    <keyword evidence="11 12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase" evidence="13">
        <location>
            <begin position="4"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="15">
        <location>
            <begin position="106"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="14">
        <location>
            <begin position="295"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="108"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EHS87416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="432" mass="48202" checksum="251B0B3A9233409E" modified="2012-04-18" version="1">MSTYYFVGIKGTGMASLARLLKDQGHEVLGSDIEKETFTQAPLEAAGIKILPFNPDNLKPGMIVVQGNAFNDDHPEIVQAHAMGLTVQSYPATVEEQVQRYTSIGIAGAHGKTSTTGLLSHVLAKLAPTNYLIGDGVGRGVADAQFFVFEADEYRDHFLAYHPDYAIMTNVEFDHPDYFKDLADVVESFKAYSQQVKKAVFAWGEDPQLRQMTFDVPVYYYGTNEQDDFRAVNIQRTASGSAFDVYHQDQLIGHFNIHLYGQHSVLNALAVVALAYTEQLDMAVVAEVLQSFNGVKRRFSETDKGDIAIIDDYAHHPSEIKATLDAARQKYPDRELVAVFQPHTYSRLKAYLNEFGESLKKADQTYVTPVFGSIREQAGDISNQDLIDLIPNGHAIDEHTLDQLLQYHQAVVVFMGAGDIEKYEDQYEKLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI88</accession>
    <name>H4GI88_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_5706</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121772.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5706"/>
    <dbReference type="EnsemblBacteria" id="EHS87309">
        <property type="protein sequence ID" value="EHS87309"/>
        <property type="gene ID" value="PS3_5706"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.248"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="322"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="353"/>
            <end position="360"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="521" mass="58292" checksum="1C485BC290A54241" modified="2012-04-18" version="1">MAVLDVNGLTMSYADKKLYDQASFQLERGEHMGIVGENGAGKSTLIKILIGQVLPVAGKITWQKNLKIGYLDQYVDIKAGMTLIEFLQTAFQDLYAKNDQMNQLYADYAEKLDDHLLNQAGQLQADLDAHHFYEIDTQIERVMTGLGLIELGRDHIVAEMSGGQRSKIILAKMLLEEPDVILLDEPTNYLDTAHIEWLIEYLSNYDGSAMIISHDYDFLERVTNTICDVAFGKITKYRGSFQQAMRQKTERQALQAREYEKQQEVIDKAQRFIRKNKAGSKSTMAKSREKMLARMDRIDPPSDNVQANFNFPYENTGSANALRVENLSAGYQRPLLAPVTFSMSSGEKILFSGFNGVGKSTLIKTILGEIPALGGTASFSPSTIVNYFDQDLIWDDPSLSPLQTLQNLFPTMLPKTIRQRLARAGINAANTMKPLQLLSGGEQTKVKLAILELTPANFLILDEPTNHLDEPTKAALKQALQSFKGSLILVSHEQSFTNGWFDKVLNVEKLSLKGEKHAKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI98</accession>
    <name>H4GI98_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Inosine-uridine nucleoside</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_10537</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121782.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10537"/>
    <dbReference type="EnsemblBacteria" id="EHS87319">
        <property type="protein sequence ID" value="EHS87319"/>
        <property type="gene ID" value="PS3_10537"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.263"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901564">
        <property type="term" value="P:organonitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023186">
        <property type="entry name" value="IUNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304">
        <property type="entry name" value="PTHR12304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="SSF53590"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IU_nuc_hydro" evidence="3">
        <location>
            <begin position="6"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="319" mass="34588" checksum="24FC58C2AA8D2EBE" modified="2012-04-18" version="1">MAKKKMILDLDTGVDDALAIAYAVAAPDVDLIGIVASYGNNLLDVCAENSLKLLELLGQTDVPVYKGLPHASTSDHFEVMQVSLDIHGNNGIGDVDLPKPQRAVEKLSGVDFYIQAAHQYGQDLIIIPTGPMTNLAAALEKDPEIADLIGNVTFMGGALTVEGNVTDVAEANINQDAKAANTVLTSSLPLTMVGLDVTLRTLLTKQETAQWRALGTKAGQAYADLTDFYIDAYYNLDIDKNGCALHDPLAVGVSLDPSFVQTLSLFMKVIYDDHNYARTIGDKDKLNDPNPNVKVAITVDKERYLATFMNYLTQLFKEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIB8</accession>
    <name>H4GIB8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_16868</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16868"/>
    <dbReference type="EnsemblBacteria" id="EHS87339">
        <property type="protein sequence ID" value="EHS87339"/>
        <property type="gene ID" value="PS3_16868"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.292"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="PTHR43827"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="4">
        <location>
            <begin position="33"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="3">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="283" mass="32630" checksum="6090C8ADF3F65230" modified="2012-04-18" version="1">MDIINSYFELADGNRMPREGFGVYKITDQTILNQSIQAAYQTGYRLFDTAQMYGNEAMLGNALKDLDVNRDELFITTKVSEANQGYDQTVKSVQQSLKDLQLEYVDLLLVHWPIHQHFFETWRAFEDLKKMGLTKSIGTSNYGMGHLQLLATKAREMPTVNQLERHPWFNQSAMIKFDQDNHIITQAWAPLGRGRMMSNPSLVSIAESHEKSVAQVILRWQLQSDIAFIPKSSHTERIQENASIFDFELTSDEIKQIDSLNQNTRISQEPELVYELGHQYPHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIC3</accession>
    <name>H4GIC3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphoglycerate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_4119</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121807.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4119"/>
    <dbReference type="EnsemblBacteria" id="EHS87344">
        <property type="protein sequence ID" value="EHS87344"/>
        <property type="gene ID" value="PS3_4119"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.300"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="4">
        <location>
            <begin position="6"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="5">
        <location>
            <begin position="107"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="318" mass="34951" checksum="CFE3DE1D191CBFA9" modified="2012-04-18" version="1">MSKAKVLVTGIIPEQGLTELKEVFDVFYDPEVETREWVLEHLKEYDGLLLMGTKADKELIDAGTNLKIITANGVGFDHIDIEYAKEKGIVVSNCPTGVRVPTAEMTFALLLATVRRLFFYDKVVRSGNWIDVSEQKYMGMSLQGKTLGVYGFGRIGSEVAKFCQALGMKVIYNDPHPLDAKLEAELDVKYADFDTLVKEADVITLHAPALPSTTGIFNAKVFDEMKDTAYIVNAARGVLIKQDDLIDALKNHKIAGAGLDVFETEPEVPVELRELDNVIMSPHAGTGTLEARTAIAKEASNNLISFLRDGKPSNQVNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GID8</accession>
    <name>H4GID8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metal-dependent membrane protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_3223</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0177 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3223"/>
    <dbReference type="EnsemblBacteria" id="EHS87359">
        <property type="protein sequence ID" value="EHS87359"/>
        <property type="gene ID" value="PS3_3223"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.328"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="153"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="218" mass="24983" checksum="B9DFE4084E731C71" modified="2012-04-18" version="1">MIRILWLFLLYLVISIPPVFLMIMGNCEKNIRLELFFGAIALIAYGLIIWWVASYYPKYRRWPVVTMSNGKKALWVLGGLVIGRVLVSLFSWLNEVLAHQGETANDMQINQIMNGNKISVTFVVISLVFMAPVVEELIFRGLIMNLFFKDERFWWPIILSATLFSACHASTTIYSFLIYFSLGCVLAFIYRKTGNIKLSIGVHFLNNVVGFIPMLLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIG7</accession>
    <name>H4GIG7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Beta sliding clamp</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PS3_22316</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms a ring-shaped head-to-tail homodimer around DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the beta sliding clamp family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121851.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000017.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_22316"/>
    <dbReference type="EnsemblBacteria" id="EHS87288">
        <property type="protein sequence ID" value="EHS87288"/>
        <property type="gene ID" value="PS3_22316"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.367"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00140">
        <property type="entry name" value="beta_clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001001">
        <property type="entry name" value="DNA_polIII_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022635">
        <property type="entry name" value="DNA_polIII_beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022637">
        <property type="entry name" value="DNA_polIII_beta_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022634">
        <property type="entry name" value="DNA_polIII_beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30478">
        <property type="entry name" value="PTHR30478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00712">
        <property type="entry name" value="DNA_pol3_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02767">
        <property type="entry name" value="DNA_pol3_beta_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02768">
        <property type="entry name" value="DNA_pol3_beta_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000804">
        <property type="entry name" value="DNA_pol_III_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00480">
        <property type="entry name" value="POL3Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00663">
        <property type="entry name" value="dnan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 11 15" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="7 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_pol3_beta" evidence="12">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol3_beta_2" evidence="13">
        <location>
            <begin position="136"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol3_beta_3" evidence="14">
        <location>
            <begin position="253"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EHS87288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="379" mass="41306" checksum="BE52EA139E414D64" modified="2012-04-18" version="1">MKFTINRLAFINALNNVLRAVSSKTTIPILTGLKLVVENDQIILTGSNADISIETTINQTDGEYALVVSEPGAIVLPARFFIEIIKKLPDQEVSIAINDGFQAVITSGTAEFTVNGQDANNFPHLPEVDGTSQVMIAPDILREVIEQTRIAVSKQESRPILTGIHFTLANGQLTAVATDSHRLAQRNVAVPTSEGQQYDVVIPGNSLTELSRMVNEVDGDVTMNFTDNQILFTFGQTKFYSRLLEGNYPETSRLIPTSSETTIELAADSFLASIERASLLSHQSRNNVVKLTINPSLSVAKISGNSADVGNVEEAINAELMAGNDLEISFNPDYMRDALKSFGSAKILMSFTSALRPFTLVPADDHDNFVQLITPVRTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIJ1</accession>
    <name>H4GIJ1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="12">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="12">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">alaS</name>
        <name evidence="15" type="ORF">PS3_4596</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="12"/>
    <dbReference type="EMBL" id="AICN01000020">
        <property type="protein sequence ID" value="EHS87270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121875.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000020.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4596"/>
    <dbReference type="EnsemblBacteria" id="EHS87270">
        <property type="protein sequence ID" value="EHS87270"/>
        <property type="gene ID" value="PS3_4596"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.396"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="SSF101353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12 15" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II_ALA" evidence="14">
        <location>
            <begin position="7"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="734"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="572"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="576"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="674"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="678"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EHS87270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="882" mass="98694" checksum="D2812DD90597C9BA" modified="2012-04-18" version="1">MKELKKLNSAQIRRMYLDFFKAKGHTEMPSQSLVPVDDPTLLWINSGVATMKKYFDGEVVPDNPRMTSSQKSIRTNDIENVGKTARHHTMFEMLGNFSVGDYFKEKAIPWAWELLTSDEWYGLDPEKLYITYYPKDDVAKEVWLKTGVAPDHLLPNQDNFWDIGQGPSGPDTEIFYDRGQKMNNLPEDDPESYPGGENERYLEIWNIVFSQFNHTPEDTYEPLPNKNIDTGMGLERVVSIFENAPTNFETDLFLPIIKQTETFSDEKQYGQDKTDDIQFKIIADHIRAITFAINDGALPSNVGRGYVIRRLIRRAVVAGKKLNIDEPFLAQLVPTVGEIMKDYYPDVLKNAEYVASVVKSEEDRFSSTLNGGLNLLNEVIAEAKADQTNSIDGRTAFKLYDTYGFPFELTREYAEDEGLVVDEDGFQAAMTEQQNRARNARDVDNGMGVQNDLWTDFKEASQYVGYTDLIVKDAKVLAMALEGQRVDTAQAGQTVEVIFDQTPFYAEMGGQVADTGDVIDNYGQTVGRVVDVQHAPNQQNLHRIELTANLKVGTRYQLVVDQKRHQKIEKNHTATHLLDQALRNVLGGHTQQAGSLVEQHYLRFDFNHFGQVTADDLQKVEDMVNEQIWREIPVTTVETDLESAKEMGAIALFSDKYGDRVRVVKIGDFNTEFCGGDHVKNTNELGLFKIVSEAGVGAGVRRIEAVTASDAFQFMQERVTLLNSVADQLKVAQLKELPHRIEDLQNELKQVQRANEQLEAKLASQQAGAAFDQVQTTNQGTLIAAELNVAGMDQLRQLADQWRQKQQSDFLVLATAVDDKVNLLVAVSDEKSHQVKAGDLIKAIAPAIKGGGGGRPTLAQAGGKNPAGISKALQEAADYLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIJ2</accession>
    <name>H4GIJ2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_13757</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000020">
        <property type="protein sequence ID" value="EHS87271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121876.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000020.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13757"/>
    <dbReference type="EnsemblBacteria" id="EHS87271">
        <property type="protein sequence ID" value="EHS87271"/>
        <property type="gene ID" value="PS3_13757"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.397"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="240" mass="28510" checksum="6B29533943A7C7A1" modified="2012-04-18" version="1">MTDKLRTGNPIWVYYRNQDEAYNIQPPKLLQGYYGESYHIEIPRFPRHQFMSASDNLEGQFDDQARQIYLYYRNANWQSNEAATNYLIASKPIQLYDRPQGLPIDNQLPDDIIFECFRKVITTDRHHWYQVTAERWLEFDPESMQLLDDNPYTDHVEPTTETDFDDQMQILSLNQASAHIDFIPDHQVPCYDYVYGKELYQLTHGTEITLLRQLTDTNGVNWYETSKHEYINALYVTINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIJ4</accession>
    <name>H4GIJ4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ATP-dependent Clp protease ATP-binding subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_13746</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000020">
        <property type="protein sequence ID" value="EHS87273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13746"/>
    <dbReference type="EnsemblBacteria" id="EHS87273">
        <property type="protein sequence ID" value="EHS87273"/>
        <property type="gene ID" value="PS3_13746"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.399"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UVR" evidence="6">
        <location>
            <begin position="252"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="645" mass="71832" checksum="41086074D5251CA7" modified="2012-04-18" version="1">MGQYGINMTDLARQGKIDPVIGRDKEIARVVEILNRRTKNNPVLIGEAGVGKTSVVEGLAESIVSGQVPEKLANKEIIRLDVVSLVQGTGIRGQFEQRMQQLIQEVSKNDDIILFIDEIHEIMGAGNAEGGMDAGNVLKPALARGEFQLIGATTLNEYRQIEKDAALARRFQPVEVAQPSVEETIQILNGIKPRYQDYHHVKYTDDAITAAAKLSDRYIQDRFLPDKAIDLLDEAGSRKNLTLKTVDPRTIENQIHIAEAHKQQAAENQDYEKAAFYRDQVQKLEKAKKDAEENHTEEAAVVTEQDMQKIVEQMTNIPVGDMQKQEANQLRDLDKQLGAHVIGQQEAVDKIARAIRRNRIGLNKSGRPIGSFLFVGPTGVGKTETAKQLAKLLFGSTDAMIRFDMSEYMDKTSTSKLIGAAPGYVGYEEAGQLTEQVRRHPYSLILLDEVEKAHPDVMHMFLQILDDGRLTDSQGRTVSFKDTIIIMTSNAGTGDMVASVGFGSTNNNKSVLDKLTNYFKPEFLNRFDDIVEFNALTEEDLGQIVQLMITDMNQMLADRDLHVHVSKAVEGKLVELDFDPKMGARPLRRVIQEQIEDKLADYVLDHDEKHNLEAELIDDAIVIKAASEPTTATATQDQSETTVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIJ8</accession>
    <name>H4GIJ8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="4 5">2.7.7.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">UDP-glucose pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_8086</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>alpha-D-glucose 1-phosphate + H(+) + UTP = diphosphate + UDP-alpha-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; nucleotide-sugar metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the UDPGP type 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="4 5"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121882.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8086"/>
    <dbReference type="EnsemblBacteria" id="EHS87193">
        <property type="protein sequence ID" value="EHS87193"/>
        <property type="gene ID" value="PS3_8086"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.407"/>
    <dbReference type="UniPathway" id="UPA00215"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02541">
        <property type="entry name" value="UGPase_prokaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005771">
        <property type="entry name" value="GalU_uridylyltTrfase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43197">
        <property type="entry name" value="PTHR43197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01099">
        <property type="entry name" value="galU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="6">
        <location>
            <begin position="6"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="303" mass="33913" checksum="34BC3C5CB723FA5D" modified="2012-04-18" version="1">MKKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEEARKSGIEDIVVVDGKNKRSIEDHFDSNPELEDNLREKHKDEMLKLVEETTDINIYFIRQSHPRGLGDAVLTARAFIGDEPFVVMLGDDLNNINNDGKPLTQELIDSYEETGASTLAVMRVPHEDTAKYGVINPSAEVKPGLFNVTSFVEKPAPKDAPSDLAIIGRYVFTPEIFDVLANTKPGKGDEIQLTDAINTLNKTQRVFAHEYKGKRYDVGNKFGWIETNIEYGLNHPETKTELREYIKELGAKFVKEDSKASKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIN5</accession>
    <name>H4GIN5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="5">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_13618</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Genetic information processing; DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribonucleoside diphosphate reductase small chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121919.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13618"/>
    <dbReference type="EnsemblBacteria" id="EHS87230">
        <property type="protein sequence ID" value="EHS87230"/>
        <property type="gene ID" value="PS3_13618"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.449"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005971">
        <property type="term" value="C:ribonucleoside-diphosphate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01049">
        <property type="entry name" value="RNRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026494">
        <property type="entry name" value="RNR_NrdF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033909">
        <property type="entry name" value="RNR_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000358">
        <property type="entry name" value="RNR_small_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23409">
        <property type="entry name" value="PTHR23409"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00268">
        <property type="entry name" value="Ribonuc_red_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04171">
        <property type="entry name" value="RNR_1b_NrdF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="333" mass="38760" checksum="C83D14299A6B5E1D" modified="2012-04-18" version="1">MSLTHYDAINWNQVSDMIDKATWEKLTEQFWLDTRIPVSNDLDDWRQLDDDHKWVVGHVFGGLTLLDTLQSEEGLKALKENVQTQHETAVLNNIQFMESVHAKSYSTIFSTLNTPDEIDEIFEWSDSQEFLQNKAIKIGNLYLDANTDPLKKKVANVFLETFLFYSGFYTPLYYLGHNQLNNVAEIIKLILRDESVHGTYIGYKFQVGMRQLSEKQQQDMKDWLYNFLYDLYDNEEKYTHMLYDQVGWTEEVLTFIRYNANKALMNLGQDPLFPDTSSDVNPIVMNGISTTTSNHDFFSQVGNGYRLGEVEAMNDDDYHVARPNSDKDLNDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIP7</accession>
    <name>H4GIP7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cytidine 5'-triphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Cytidine triphosphate synthetase</fullName>
            <shortName evidence="9">CTP synthetase</shortName>
            <shortName evidence="9">CTPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pyrG</name>
        <name evidence="12" type="ORF">PS3_13446</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + NH4(+) + UTP = ADP + CTP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="9">CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the CTP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121931.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13446"/>
    <dbReference type="EnsemblBacteria" id="EHS87242">
        <property type="protein sequence ID" value="EHS87242"/>
        <property type="gene ID" value="PS3_13446"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.466"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01227">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="PTHR11550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP_synth_N" evidence="11">
        <location>
            <begin position="3"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="GATase" evidence="10">
        <location>
            <begin position="303"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Amidoligase domain" evidence="9">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="L-glutamine binding" evidence="9">
        <location>
            <begin position="383"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutamine hydrolysis" evidence="9">
        <location>
            <position position="382"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="509"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="511"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="14"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="148"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="188"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="406"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="533" mass="59443" checksum="773A8BF01CD3C558" modified="2012-04-18" version="1">MTKYIFVTGGVVSSLGKGIVAASLGRLLKNRGLKIAIQKFDPYINVDPGTMSPYQHGEVFVTDDGTETDLDLGHYERFIDNNLNRYSNVTTGKIYSEVLRKERRGDYLGRTVQVIPHITNMIKDKIQRAGESTDAEVVITEIGGTVGDIESQPFMEAIRQMKKEKGSENVLYIHTTLIPYLRAAGEMKTKPTQHSVRELRGLGIQPNILVVRTEEHITDEMRAKIADFCDVAPEAVIESMDASVLYEVPLMLQAQKMDQLVVDHFGLNVPAADMQAWQEMVDHIKHGLTKEVKIAMVGKYTDLQDAYISVNESLRHAGFPVNAKVKIDHFNAEHINSDNVKEVLGSYDGILVPGGFGNRGVEGMITAIKYARENDVPYLGICLGMQTAVIEYARDVLGYQDANSTEFDPNTAHNVIDLMSDQESVEDMGGTQRLGAYTCQLVPGKIAAAAYDNQTTISERHRHRYEFNNDFRQELEDHGLVISGINPERNLVEVVEIPQNRFFVAAQYHPEFLSRPNHPEGLFAAFVKAAARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIQ3</accession>
    <name>H4GIQ3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Bifunctional protein GlmU</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="15">UDP-N-acetylglucosamine pyrophosphorylase</fullName>
                <ecNumber evidence="15">2.7.7.23</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="15">N-acetylglucosamine-1-phosphate uridyltransferase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="15">Glucosamine-1-phosphate N-acetyltransferase</fullName>
                <ecNumber evidence="15">2.3.1.157</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="15" type="primary">glmU</name>
        <name evidence="17" type="ORF">PS3_11895</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate + UTP = diphosphate + UDP-N-acetyl-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13725"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="EC" id="2.3.1.157"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="15">Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="15">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="15">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">In the C-terminal section; belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.23" evidence="15"/>
    <dbReference type="EC" id="2.3.1.157" evidence="15"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121937.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11895"/>
    <dbReference type="EnsemblBacteria" id="EHS87248">
        <property type="protein sequence ID" value="EHS87248"/>
        <property type="gene ID" value="PS3_11895"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.473"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00532"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00973"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019134">
        <property type="term" value="F:glucosamine-1-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03353">
        <property type="entry name" value="LbH_GlmU_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01631">
        <property type="entry name" value="GlmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005882">
        <property type="entry name" value="Bifunctional_GlmU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038009">
        <property type="entry name" value="GlmU_C_LbH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01173">
        <property type="entry name" value="glmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10 15" id="KW-0133">Cell shape</keyword>
    <keyword evidence="14 15" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="16">
        <location>
            <begin position="6"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyrophosphorylase" evidence="15">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-GlcNAc binding" evidence="15">
        <location>
            <begin position="9"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-GlcNAc binding" evidence="15">
        <location>
            <begin position="78"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="UDP-GlcNAc binding" evidence="15">
        <location>
            <begin position="101"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Linker" evidence="15">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-acetyltransferase" evidence="15">
        <location>
            <begin position="252"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Acetyl-CoA binding" evidence="15">
        <location>
            <begin position="386"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="363"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EHS87248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="455" mass="49320" checksum="2538A5EC139D3D02" modified="2012-04-18" version="1">MTTRNAIILAAGKGTRMKSKLHKVLHEVCGKSMVEHVLSQLQLAGINKIVTVVGFGADDVKHTLGDQVAYALQKQQLGTGHAVMQTESLLGQSEGETLVVSGDTPLFTAATFDRLFDYHEQRHAAVTILTSKAPDPTGYGRIVRNEVGIVERIVEQKDATSEEQDIHEINTGVYCFDNQKLFAALKKLTNDNAQGEYYLTDVIGILKSQNEVVTAYQMDDFEESMGVNDLVALAKARRIMQERINTKLMKDGVSLIDPDTTYIDADVKIGNDTVIEGGVVIKGESRIGSDCFIGAHSRIEDSVLHDEVKVISSTLEKAEMHTGSDIGPNSHLRKGAELGKDVHVGNFCEVKNAYLGEGTKMGHLSYVGDATLGKNINIGSGVIFVNYDGANKAHTNVGDHVFIGSNSNLVAPVNIAADSFIAAGSTITDDTAQYDLAIARQRQTNKANYAKKLPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIQ4</accession>
    <name>H4GIQ4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pur operon repressor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_14296</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121938.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14296"/>
    <dbReference type="EnsemblBacteria" id="EHS87249">
        <property type="protein sequence ID" value="EHS87249"/>
        <property type="gene ID" value="PS3_14296"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.474"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045982">
        <property type="term" value="P:negative regulation of purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015265">
        <property type="entry name" value="PuR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010078">
        <property type="entry name" value="PurR_Bsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43864:SF2">
        <property type="entry name" value="PTHR43864:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09182">
        <property type="entry name" value="PuR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01743">
        <property type="entry name" value="purR_Bsub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <feature type="domain" description="PuR_N" evidence="6">
        <location>
            <begin position="4"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Pribosyltran" evidence="5">
        <location>
            <begin position="109"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="272" mass="29948" checksum="70C6B63E97CC8BCE" modified="2012-04-18" version="1">MKIRRSNRLVDMTRYLLEHPRELVSLKFFGERYGSAKSSISEDLGIIRKTLQDWGVGKLETLPGASGGARLTPMYSADNAKMVIDQLVEKVDDESRLLPGGYVYLSDLLGDPEVLREVGRLIATQYEAQDVDVVMTIETKGIPLAQSVAMYLNRPFVIVRNSSHITEGSTVSVNYVSGSSQRIKKMELSRRTLKEGANVVVVDDFLKGGGTLNGMKSLIDEFGAHLVGMNVFAEGQFEGERLIGECTSLIKVDSSDNQFSAKPGNYLATVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIR7</accession>
    <name>H4GIR7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Exopolyphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_14294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121951.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14294"/>
    <dbReference type="EnsemblBacteria" id="EHS87262">
        <property type="protein sequence ID" value="EHS87262"/>
        <property type="gene ID" value="PS3_14294"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.487"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003695">
        <property type="entry name" value="Ppx_GppA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02541">
        <property type="entry name" value="Ppx-GppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ppx-GppA" evidence="1">
        <location>
            <begin position="19"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="310" mass="35180" checksum="7E366E419D47EF29" modified="2012-04-18" version="1">MENLAIVDLGSNSTRMAITQIDANGNFREVKRVKENTRLSEGMGAEKRLQKPAMDRTIAALKRFKRIYRSYSQLSVRCISTAAVRQAVNQQEFLDRVQNEVGLTLQVLTGKQEAYYDYLGVERTLKMDHCLILDVGGASSELVLVQHKKARDMISLPFGAVNLSEQFQLGGYVRANDLFRAETLVTNRFNKVPWLRYANRVPIVLLGGANRTLARMSQVRRKQRPHSIHGYRMSSGAVYSIFRDMLSRNLAQRKRLPGLEYDRADIIVGGLLPLICLLQRNSDGRVIFSESGVREGIITEYIQQRGKQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIS5</accession>
    <name>H4GIS5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methionine synthase II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_5736</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000022">
        <property type="protein sequence ID" value="EHS87191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121959.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000022.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5736"/>
    <dbReference type="EnsemblBacteria" id="EHS87191">
        <property type="protein sequence ID" value="EHS87191"/>
        <property type="gene ID" value="PS3_5736"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.502"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Meth_synt_2" evidence="1">
        <location>
            <begin position="10"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="365" mass="41491" checksum="4632CB676AF06D73" modified="2012-04-18" version="1">MGQAPFKYDIIGSFLRPTKLKEAREQVRAGELSADQLKAIEDESIRQLIKQLVDHGYPVVTDGEFRRGWYHSDFLGALNGVKLSTYTMNLFGEDTLVGSTTIEGPISWNPNHPFIDHFKFAKAEADQYGVMTKLDIPGPNMVLIDTITTDQANYYGKDFKRLAQDFVQVYQDAIKSFYDAGCRYLQLDDPIWVSLCDDNFKAKIVAAGFDVDEVRDTFYQTSQAILAAKPADMDITLHICQGNLRSKKFYDTTYDDLVDTIFQLPYSGFFMEFDDEKYCNFELLKKLQGQKVVLGVVNTRTNELEDLTTLVDRVKRARQFVSKDQLCVATQCGFASTAEGNLISEEIQWQKLDLVRQVAQVALED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIT0</accession>
    <name>H4GIT0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA pseudouridine synthase A</fullName>
            <ecNumber evidence="4">5.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridine(38-40) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridylate synthase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA-uridine isomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">truA</name>
        <name evidence="9" type="ORF">PS3_2096</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121964.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2096"/>
    <dbReference type="EnsemblBacteria" id="EHS87160">
        <property type="protein sequence ID" value="EHS87160"/>
        <property type="gene ID" value="PS3_2096"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.509"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02570">
        <property type="entry name" value="PseudoU_synth_EcTruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PTHR11142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001430">
        <property type="entry name" value="tRNA_psdUrid_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="PseudoU_synth_1" evidence="8">
        <location>
            <begin position="9"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="PseudoU_synth_1" evidence="8">
        <location>
            <begin position="146"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4 5">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="260" mass="29566" checksum="CE0E99166B2D47C0" modified="2012-04-18" version="1">MYRYKITFAYDGAGFSGFQIQPHKRTVEQTLKTAVNKIAKNPDPPLHVIGSGRTDAGVHALNQVAHFDMPYLIPDESMRKALNSILPLDILVKKAERVPNDFHARYDTHSKRYRYRVDQGEFVNPFKRLYTAHYKYPLDLAKMQRAAQDLLGEHDFTSFVASGSQAQSNVRRIDDVLVKRDEINHEVIFEFEGNGFLYNQVRIMVALLLEIGNGQRPVDDIPRVLAAKDRQAARMTAPATGLYLVRVNYADSPAKNIDDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIT3</accession>
    <name>H4GIT3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC transporter ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_10174</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121967.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10174"/>
    <dbReference type="EnsemblBacteria" id="EHS87163">
        <property type="protein sequence ID" value="EHS87163"/>
        <property type="gene ID" value="PS3_10174"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.512"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030947">
        <property type="entry name" value="EcfA_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04520">
        <property type="entry name" value="ECF_ATPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="4"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="278" mass="31046" checksum="5F935A98B6DBCF4D" modified="2012-04-18" version="1">MSLVEVRNLSFTYPDNDEPVLHDLSFEIEANEWVAIIGHNGSGKSTLARLIDGLLSPDQGTIRIDQLPVDNDNLSLIHQKIGFVFQNPDNQFVGATVADDIAFGLENRQLSRPVMQDKVQRVLKRVDMVDFANHQPEQLSGGQKQRVAIAGILALTPQLLILDEATSMLDPEGRAEILNLIVELRRESKLSVLSITHDLEEALLADRVILMDKGQIIKVGTPQETVMNQQLMASHHLKPVFVRRLLDRLNAAGISAPADVNTSEQLEMWLWKQLDSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIT7</accession>
    <name>H4GIT7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">30S ribosomal protein S13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpsM</name>
        <name evidence="9" type="ORF">PS3_15044</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement. Contacts the tRNAs in the A and P-sites.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121971.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15044"/>
    <dbReference type="EnsemblBacteria" id="EHS87167">
        <property type="protein sequence ID" value="EHS87167"/>
        <property type="gene ID" value="PS3_15044"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.516"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_S13_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="30s_Rbsml_prot_S13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_S13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019980">
        <property type="entry name" value="Ribosomal_S13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_S13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF1">
        <property type="entry name" value="PTHR10871:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03631">
        <property type="entry name" value="uS13_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="88"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="91"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="121" mass="13564" checksum="92233AFF857EBD38" modified="2012-04-18" version="1">MARISGVDLPRDKRIVIGLTYIYGIGNTTAQKICAAAGVSEDVRVRDLTPDQEDKLRVQVDQLKVEGDLRREVSMNIKRLQEIGSYRGMRHRRGLPVRGQHTKNNARTRKGKAVAIAGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIU3</accession>
    <name>H4GIU3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">50S ribosomal protein L18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rplR</name>
        <name evidence="9" type="ORF">PS3_18389</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S and 23S rRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18389"/>
    <dbReference type="EnsemblBacteria" id="EHS87173">
        <property type="protein sequence ID" value="EHS87173"/>
        <property type="gene ID" value="PS3_18389"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.524"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_B">
        <property type="entry name" value="Ribosomal_L18_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005484">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004389">
        <property type="entry name" value="Ribosomal_L18_bac-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899">
        <property type="entry name" value="PTHR12899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00861">
        <property type="entry name" value="Ribosomal_L18p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00060">
        <property type="entry name" value="L18_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="121" mass="13265" checksum="FF512AEE2774115E" modified="2012-04-18" version="1">MTVVISKPDKNKIRQRRHKRVRGKISGTAERPRLSVYRSNKNIYAQVIDDVKGVTLASASTHDSEVSGNTKTEQAASVGALVAKRAAEKNISVVVFDRGGYLYHGRVQALAEAARENGLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIV4</accession>
    <name>H4GIV4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">30S ribosomal protein S19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpsS</name>
        <name evidence="8" type="ORF">PS3_14893</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121987.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14893"/>
    <dbReference type="EnsemblBacteria" id="EHS87184">
        <property type="protein sequence ID" value="EHS87184"/>
        <property type="gene ID" value="PS3_14893"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.536"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_S15/S19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_S19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005732">
        <property type="entry name" value="Ribosomal_S19_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_S19_S15_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="PTHR11880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="SSF54570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01050">
        <property type="entry name" value="rpsS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="93" mass="10488" checksum="84E27A37E0976661" modified="2012-04-18" version="1">MGRSLKKGPFADASLLKKVEAQADNEKKTVIKTWSRRSTIFPSFIGYTFAVYDGRKHVPVYVQEDMVGHKLGEFVPTRTFRGHAGDDKKTGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIW5</accession>
    <name>H4GIW5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphopentomutase</fullName>
            <ecNumber evidence="4">5.4.2.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Phosphodeoxyribomutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">deoB</name>
        <name evidence="6" type="ORF">PS3_13159</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Phosphotransfer between the C1 and C5 carbon atoms of pentose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>2-deoxy-alpha-D-ribose 1-phosphate = 2-deoxy-D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:27658"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>alpha-D-ribose 1-phosphate = D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:18793"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="EC" id="5.4.2.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="4">Binds 1 or 2 manganese ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route II): step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the phosphopentomutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.7" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121998.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13159"/>
    <dbReference type="EnsemblBacteria" id="EHS87137">
        <property type="protein sequence ID" value="EHS87137"/>
        <property type="gene ID" value="PS3_13159"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.550"/>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00173"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008973">
        <property type="term" value="F:phosphopentomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043094">
        <property type="term" value="P:cellular metabolic compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009264">
        <property type="term" value="P:deoxyribonucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16009">
        <property type="entry name" value="PPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00740">
        <property type="entry name" value="Phosphopentomut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010045">
        <property type="entry name" value="DeoB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024052">
        <property type="entry name" value="Phosphopentomutase_DeoB_cap_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21110">
        <property type="entry name" value="PTHR21110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001491">
        <property type="entry name" value="Ppentomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143856">
        <property type="entry name" value="SSF143856"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01696">
        <property type="entry name" value="deoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="5">
        <location>
            <begin position="5"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="397" mass="43664" checksum="8D81C6F3996C7471" modified="2012-04-18" version="1">MAYQRVFGIVLDSVGTGAADDANRYGDLGADTLGHVGEDYAGNLHLPNLARLGLSNLRDVPIQGVPVVKSALGCYGKMAEISAGKDSMDGHWEMMGLPVKQVLDTFPHGFPEALIQQIETFSGRPVIVNQPYSGTQVIHDYGEEQLATGALIVYTSGDSVLQIAAHEAVISVEELYRICEYVRGLVNGPEYLIGRVIARPYLGSDRDHFTRTANRRDFSLVPPEVTDLDRLQLAGYQVIGIGKINDIFSGTGINQAFHNESNQDGMQHLLDVMDTDFNGFCFMNLVDFDAMYGHRRDPQGFGQALMEFDQALGQVMERLRSDDLLMITADHGNDPGFKGTDHTRENVPLLVYSPSMQTPNQSLGTRTTFADFGATILDNFRITPGEGESFYQTIKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIY9</accession>
    <name>H4GIY9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Major facilitator superfamily permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_7452</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the major facilitator superfamily. EmrB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122022.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87101">
        <property type="protein sequence ID" value="EHS87101"/>
        <property type="gene ID" value="PS3_7452"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.593"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="272"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="442"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="9">
        <location>
            <begin position="19"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="477" mass="52155" checksum="6FB7B536B216BCCD" modified="2012-04-18" version="1">MKKIAVRDINNQPYNRLIMVIILMTGSFCTVLNQTILATAYPTLMRYFSVNTSTVQWLTTGFLMVNGMMIPVSAYLSNRLNTKYLYLGAMVVFEVGTVVCWLAPSFAILLVGRLIQALAVGVTVPLIQTIMLTIFPPEKRGTAMGYTGIVVGLAPAIGPTLSGFIIDTMTWRDLFRLIAPIVLLVIVLGWFFIHPVLANNDDKLDLRSLILSTFGFGLLLYGFSVVGSYGWSSPLVIGSLFVGTVIVAMMFYRQLKINHPFLEVRVFKHRPFIIGTTLSSIVRMAMVGVEMIIPIYLQNVHQLSAFESGLVLLPGAAMFGLLSPLTGRIFDKYGAKYLTMIGMTLLTIGTLLLVGVNQSFSIIWIALIYLVRMVGISMVMMPASTAGMNSLPLSLFADGSAVNNTIRQVAAAIGTALMTSVLDDVTRQSERHLTTSAGKLTAVLNGYHAAFLLAAGFAFIGIIIASFFKRKQERSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIZ9</accession>
    <name>H4GIZ9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ferric reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_9720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122032.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9720"/>
    <dbReference type="EnsemblBacteria" id="EHS87111">
        <property type="protein sequence ID" value="EHS87111"/>
        <property type="gene ID" value="PS3_9720"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.606"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="2">
        <location>
            <begin position="206"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="425" mass="48305" checksum="0B65C7D709FE4621" modified="2012-04-18" version="1">MLKRHGYWLGLIWMLVIFVLPLPFLQTLQVGIGGIVGAEWLPIAAGTIAYVWMLTSIYISTRPQWLDRTIGLPAAYQLHGIISLAAILLAFLHLEGAHSQGWIKLTGFWAFYLFLGVAAYSLIFMAGWLTSRVHWLASLKVWLEKVFRHEISLWLHRLNLVATILVFIHVWLIDYIRAITPFMILFTGATGLVFLAYGYYRYQAHQGHTRARLMEIKALNDQVLQLVIKLRRPLSLHSGDYIFIAFPEIAGLEELHPFSLAKIDPDRRIVTLAIRQDGDFTSQLVQLSSGQSLIVTGGYGLYHSIIQDQKPQAMILLAGGIGVVPLLSIAEQYADLPINFYYNAHSANNLLYEDLFTKWEQRNNFKSVRQVGRLATQEVINHLPDDRQGLLVLIAGPSSMAHHWIKVLKAAGIPRHQIFYEAFNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ07</accession>
    <name>H4GJ07_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SNARE associated Golgi family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122040.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13640"/>
    <dbReference type="EnsemblBacteria" id="EHS87119">
        <property type="protein sequence ID" value="EHS87119"/>
        <property type="gene ID" value="PS3_13640"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.622"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="225" mass="25509" checksum="A81BE13D29856F3B" modified="2012-04-18" version="1">MKQRLSHRALIVIATLIVIAMLGLVIHDFLPEINLLLHSNDANRQELVDLIQQHGIRDMIFLYIMIAVMNAIPGMSNSIVCIFAGLCYGPLWGLTINWFGNMTGNWLVAALIGHIDFSEKFMHNRWLERLQSSDHPWITLTVAYMIPVIPSVLVNYSCVNMQIPRRNFLVMVSLGVLPVSLIYAFGGDAIFNGEFKRLIIYAIIIIVLIMLSRLIGKNVKKRHAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ10</accession>
    <name>H4GJ10_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Xanthine phosphoribosyltransferase</fullName>
            <shortName evidence="1">XPRTase</shortName>
            <ecNumber evidence="1">2.4.2.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">xpt</name>
        <name evidence="2" type="ORF">PS3_13423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts the preformed base xanthine, a product of nucleic acid breakdown, to xanthosine 5'-monophosphate (XMP), so it can be reused for RNA or DNA synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + XMP = 5-phospho-alpha-D-ribose 1-diphosphate + xanthine</text>
            <dbReference type="Rhea" id="RHEA:10800"/>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; XMP biosynthesis via salvage pathway; XMP from xanthine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family. Xpt subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.22" evidence="1"/>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122043.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13423"/>
    <dbReference type="EnsemblBacteria" id="EHS87122">
        <property type="protein sequence ID" value="EHS87122"/>
        <property type="gene ID" value="PS3_13423"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.627"/>
    <dbReference type="UniPathway" id="UPA00602">
        <property type="reaction ID" value="UER00658"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000310">
        <property type="term" value="F:xanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046110">
        <property type="term" value="P:xanthine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032265">
        <property type="term" value="P:XMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01184">
        <property type="entry name" value="XPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010079">
        <property type="entry name" value="Xanthine_PRibTrfase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01744">
        <property type="entry name" value="XPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="1" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="5-phospho-alpha-D-ribose 1-diphosphate binding" evidence="1">
        <location>
            <begin position="128"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>xanthine</name>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>xanthine</name>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>xanthine</name>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="192" mass="21249" checksum="8CD673EC6422D429" modified="2012-04-18" version="1">MKELEDRIRQDGQVLPGNVLKVNSFLNHQIDPNLMMNMGKEFADHFKDDQITKIVTVESSGIAPAVMTGFQMQVPVVFARKHKSLTLVDHLYTADVYSYTKQTTNTISIDDRFVNADDRLLIIDDFLANGQAVEGLLKIADQAQATVAGVGIVIEKSFQKGAELIQERGIKLLSLANIASLEDGQVRFVGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ12</accession>
    <name>H4GJ12_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HesB/YadR/YfhF-family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_14608</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122045.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14608"/>
    <dbReference type="EnsemblBacteria" id="EHS87124">
        <property type="protein sequence ID" value="EHS87124"/>
        <property type="gene ID" value="PS3_14608"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.629"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="112" mass="12514" checksum="6F88EB93484C4FDA" modified="2012-04-18" version="1">MKLTVTQAASQYIIQQLQLTAGDGVKFFTSPENRQPVKHGPHQEFAQDNQADRPVALTEVDGINYHINFADNWFFASHDTTVDYHPDTGLSFTYAGIDADGGASINYEKYLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ29</accession>
    <name>H4GJ29_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">ATP synthase epsilon chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">ATP synthase F1 sector epsilon subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10 12">F-ATPase epsilon subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">atpC</name>
        <name evidence="17" type="ORF">PS3_13437</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12 13">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cell membrane</location>
            <topology evidence="2 12">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12 13">Belongs to the ATPase epsilon chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122062.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13437"/>
    <dbReference type="EnsemblBacteria" id="EHS87054">
        <property type="protein sequence ID" value="EHS87054"/>
        <property type="gene ID" value="PS3_13437"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.666"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12152">
        <property type="entry name" value="F1-ATPase_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00530">
        <property type="entry name" value="ATP_synth_epsil_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036794">
        <property type="entry name" value="ATP_F1_dsu/esu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001469">
        <property type="entry name" value="ATP_synth_F1_dsu/esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020547">
        <property type="entry name" value="ATP_synth_F1_dsu/esu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020546">
        <property type="entry name" value="ATP_synth_F1_dsu/esu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036771">
        <property type="entry name" value="ATPsynth_dsu/esu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13822">
        <property type="entry name" value="PTHR13822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00401">
        <property type="entry name" value="ATP-synt_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02823">
        <property type="entry name" value="ATP-synt_DE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46604">
        <property type="entry name" value="SSF46604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51344">
        <property type="entry name" value="SSF51344"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01216">
        <property type="entry name" value="ATP_synt_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 12 13" id="KW-0139">CF(1)</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 12 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATP-synt_DE_N" evidence="16">
        <location>
            <begin position="6"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_DE" evidence="15">
        <location>
            <begin position="92"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EHS87054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="142" mass="15174" checksum="AB33984E2008E1A2" modified="2012-04-18" version="1">MAEHSFQVTISTPDGVVYDQPATMVVVTTAGGQMGVMANHVPVVAALGIDLVTVKHSDTDAADDVIAVNGGFMEFHNNVATIAADSAELAQDIDINRAESAKERAEKMLNQANASHNKSELDRAEVHLKRAINRLKATNRPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ53</accession>
    <name>H4GJ53_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Membrane protease subunit, stomatin/prohibitin family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_22054</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_22054"/>
    <dbReference type="EnsemblBacteria" id="EHS87078">
        <property type="protein sequence ID" value="EHS87078"/>
        <property type="gene ID" value="PS3_22054"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.695"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="PHB" evidence="2">
        <location>
            <begin position="49"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="279" mass="30804" checksum="0BEE1CE57AEF3058" modified="2012-04-18" version="1">MIKEKNVFHINGYAGLLIALILLALSIWAFFSSIVLAVILFILSFILSSGLVIVQPNEAVVLTFFGSYLGTIREPGLFMTIPFTMNQKVSLRIDNFNSAVIKVNDMMGNPVEIAAVVVYKVVDTAQARFAVEDYGQFVEIQSESAVRHVASEYPYDTLEGDDQLTLRGNPTEVSQRLTQELQERLRVAGVEVVETRLTHLAYATEIAAAMLQKQQSTAILSARKIIVEGAVKITEDAIDRLAKETNLSLTDEQKLQVINNIMVAIISERGTQPVINTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ57</accession>
    <name>H4GJ57_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Lysine transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_8616</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122090.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8616"/>
    <dbReference type="EnsemblBacteria" id="EHS87082">
        <property type="protein sequence ID" value="EHS87082"/>
        <property type="gene ID" value="PS3_8616"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.703"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amoino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="448"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="AA_permease" evidence="7">
        <location>
            <begin position="21"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="502" mass="54688" checksum="ACBA42E688E35D1D" modified="2012-04-18" version="1">MATDTSHQQGGEIKRSLQSRHLSMIALGGSIGTGLFIASGSAITTAGPGGALVAYAVMGIMVYFLMTSLAEMATYIPLSGSFAAYATKFVSPAMGFALGWNYWLNCAITVPVEVTTAGLVMNFWFPHIPSWIFSAVVFALIFLINVLSVKSYGETEFWLALVKVVTVIVFLIVGVLVILGIMGGQGPVGLENFTYKKAPFVNGFSGILSVFLVAGFSFQGTEMVGIAAGESADPEKSVPRAIHSTFWRILLFYICTIFVIACILPYTNPNLLSSDVKDIVTSPFTLVFKRAGLAAAASVMNAVILVAVLSSANSWMYASSRMLYSQAREGYAWKILGYIDKRGIPIYGLLSTLVIGILVWLTQFIGPEAYNYLIGASGLSGFIAWVGIAVSHWRFRRAFMAQGHQLSELKYHAALFPLGPILAFVLCLVVICGQNIDAFINMDWSNIAITYMTVPIFIILYIYYKVKYGSKLIPLKKVDLSRRTKGEAFEPADEPTGERQNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ95</accession>
    <name>H4GJ95_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_18913</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000030">
        <property type="protein sequence ID" value="EHS87020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18913"/>
    <dbReference type="EnsemblBacteria" id="EHS87020">
        <property type="protein sequence ID" value="EHS87020"/>
        <property type="gene ID" value="PS3_18913"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.760"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="309" mass="35415" checksum="00A22147215B2F4C" modified="2012-04-18" version="1">MKTMSNQTPKSSLALMKQLSHSIKQGLATGERYVELLNVLANDQDPAVLLDATKDLTKLDLTSDYVKLPEHYNFADYYLIFIDRLLGLLKAQNTRLQGDHNDLRMQIAELGEDVAFKFERRIETNEVCFADQEYHLALFSLDLEHRVLGFNSQALVDFFVANHSYQTANDLQTAVAPLINLAKFVQNELNFTIDLGILDTDNQFGYYLNRPDLHTTVIDKLFVATADTGYFLMNLPESNGAELELENQVKLQIKFDPQLEEPQWYFMVQDQASRVSFFDLLLSSDLIQKWYLDNRDDLAVRPSNLSGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJA9</accession>
    <name>H4GJA9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_4075</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000032">
        <property type="protein sequence ID" value="EHS86999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122142.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000032.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4075"/>
    <dbReference type="EnsemblBacteria" id="EHS86999">
        <property type="protein sequence ID" value="EHS86999"/>
        <property type="gene ID" value="PS3_4075"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.802"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022567">
        <property type="entry name" value="DUF3459"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="Glyco_hydro_13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11941">
        <property type="entry name" value="DUF3459"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aamy" evidence="3">
        <location>
            <begin position="14"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="562" mass="65287" checksum="7E96D4832F2DC1C4" modified="2012-04-18" version="1">MENQHWWSNSVVYQIYPKSFQDSNGDGIGDLPGIIQRLDYLAKLGVDIIWLNPIYASPQVDNGYDISDYHAIDPAFGTMDDFKKLVELAHQKGIKIVMDLVVNHTSDQHHWFIESNRSTNNRYRDFYIWRDADPEHAPNNWGSYFSGSAWHYDPQTKQSYLHLFAPEQPDLNWENPLVRQEVYQMMNWWAELGVDGFRMDVISLISKPAGLPDAPKSDNSQYGNPETMVANGPRVHEFLQEMHQAVMDHHQMVTVGETPGVTTKEARKYANLNDRELNMVFQFEHMSLDGNPNPQLGKWYDQKVKLSLLRENLSKWQTELAGQAWNSLYWNNHDQPRAVSRFGDDSTETYREKSAKMLATILHFQQGTPYIYEGEEIGMTNYPFHDLSDYNDLESINAYHQLVDQAHLLDGETMLKYLAFHSRDNARTPMQWDDTTHAGFTTGQPWLPVNPNHSQLNVQQALADPNSIFYYYQRLIKLRHELPVITNGQYALVAGNLEDPDVYAYTRDDGQQRLWIIANFTKQTLQRDFSIPKAATRLISNYDDDQLITLRPYEAKVYQQIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJB3</accession>
    <name>H4GJB3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Polyketide transporter ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_4235</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000032">
        <property type="protein sequence ID" value="EHS87003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122146.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000032.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4235"/>
    <dbReference type="EnsemblBacteria" id="EHS87003">
        <property type="protein sequence ID" value="EHS87003"/>
        <property type="gene ID" value="PS3_4235"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.806"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="300" mass="34041" checksum="1D0396DC9DD19E89" modified="2012-04-18" version="1">MAVLKVEHLTKDFGHGRGIFDVNFEINKGEVLGFLGPNGAGKSTTIRHLMGFTKPGQGKVEINGFDCTNKPQEVMKYVGYLPGEVALPDDMTGAQFIKMMKAMRHIKDDRYLNHLIELFDLDYRGSMKKMSLGEKRKLAVVVAFMADPAILILDEPTSGLDPVMQEKFIEFMVEEKRRGKTILLSSHIFSEVEATCDRINIIKEGSIVAEVDSADLKNEKQKNFQLGFTNPSNLAQAMSVFDNKKIVQPLVLEVTVAKERISDFFKQLADLKVVAINERRFNLEKYFLDFYRSDKVFEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJB7</accession>
    <name>H4GJB7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_18761</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000032">
        <property type="protein sequence ID" value="EHS87007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122150.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000032.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87007">
        <property type="protein sequence ID" value="EHS87007"/>
        <property type="gene ID" value="PS3_18761"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.814"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="2"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="289" mass="32357" checksum="3D741F03E190DCDD" modified="2012-04-18" version="1">MIDYQLLEELVTFQETGTLSATAEKLLITQPSVTRGMQKLEDELGVSLFDRTVNRLKLNSTGELAAKLARKALAANQDFVTITQNYARQQNAITIASIAPGPLLLLNYLQTQNDELTNIVLKPVMSVTDVKESLLSAESQLAFITDPLDSDHIIEEPIGVEQLYLSISTFHALASYPKVTFDDLADMSFILSDDIGIWHDIIQNAIPNLHPLVQNSEEAIQQLTSFSDFPAFNSNITQLTSNRNTDNRKSVLIDDDRARMKFFAAYVNEQADRVKPIIELMKALWPTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJB9</accession>
    <name>H4GJB9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Nramp family metal ion transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_12436</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000033">
        <property type="protein sequence ID" value="EHS86988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122152.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000033.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12436"/>
    <dbReference type="EnsemblBacteria" id="EHS86988">
        <property type="protein sequence ID" value="EHS86988"/>
        <property type="gene ID" value="PS3_12436"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.820"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="PTHR11706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="341"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="376"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS86988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="403" mass="42449" checksum="88BE950832139A53" modified="2012-04-18" version="1">MQETTSNRHRQLRNAAIGGAFLMAMSAVGPGFLTQTATFTQSEGANFGFAILICIIVDVIVQLNIWRIIVVAGERAQVVANQVLPGLGYLLSLLVAIGGLFFNIGNVAGAGLGLNVMFGIPVTTGAIISGLAGIIILIIKNAMPVVDRTVQILAIAAVLVLVYVLCVFKIPYTTAVTHTFAPTKINFYSILTIVGGTVGGYISFSGGHRLLQGGAKGQKDTHYVNLGALTGIGLASLIRVLLFLVGLGVVLAGHQLAADNPAATIFQVAAGQIGYKFFGLLLFAAGMSSVIGSTFTSTSFLNYATNHDQEAKETTTQRWLTIGFIAISTLIYIFIGQPAKVLVFVGALNGTILPIALGILLLASRKQKVMGDGYRHPIWLSITGWIVVVFMAYAACNTIAGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJE0</accession>
    <name>H4GJE0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative galactoside O-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_11639</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000035">
        <property type="protein sequence ID" value="EHS86889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122173.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000035.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11639"/>
    <dbReference type="EnsemblBacteria" id="EHS86889">
        <property type="protein sequence ID" value="EHS86889"/>
        <property type="gene ID" value="PS3_11639"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.849"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="182" mass="19918" checksum="EFE111A74D8489E6" modified="2012-04-18" version="1">MTEQLDLRNQPAERRAEIARTRELVFKLNHQLPQSSEYLATLRELFTSGLGEHCFIEGPIYINLAANLKIGNRVSINPYFKCMAAGKITIEDGAKLAMGVSIITNNHDLYEREILTIEPVHIKANAWIGANVTILPGVTIGKNAVIGAGSVVTHDVPDNTVVVGNPARPLKTLESSKFMKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJG1</accession>
    <name>H4GJG1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cation-transporting ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_5340</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000037">
        <property type="protein sequence ID" value="EHS86722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5340"/>
    <dbReference type="EnsemblBacteria" id="EHS86722">
        <property type="protein sequence ID" value="EHS86722"/>
        <property type="gene ID" value="PS3_5340"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.911"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="SSF81660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="786"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="828"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="861"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="898"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="domain" description="Cation_ATPase_N" evidence="8">
        <location>
            <begin position="11"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS86722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="927" mass="101900" checksum="0CCD0B8BCC640092" modified="2012-04-18" version="1">MSEDHDQIITIAQIDDPNLIYQQMQTRSAGLNEEEVSQRQRQYGKNILKTKAGEPIWLKFLKNFSSTMAILLWISGAIAFLAQLQELGIAIWAVNLINGCFSFWQEYQAGKATEALSKMLPAHSRVVRAGTDVKILAEDLVPGDIIKLEEGDDIPADIRLLQTTDVQVNQSSLTGEVNPVHKNERPVTNPKKNHFELTNMVFSGTSMMKGNATGVVVTTGMATDFGKIAALTQNVHDDQSPLQKELSVLTKQISILAVSIGLIFFLVATFFVHYPLVKSFIFALGMIVAFIPEGLLPTVTLSLAGAVQQMAKKNALVKKLSSVETLGSASVICSDKTGTLTQNQMTVSDLWTVQKHYRVDGEGYAVNGKIYDGPHQVNYQSSPDLYELLRGGLLADNARIMPPDDQHPRYTVLGDPTEACLEVVAAKGELDLKAERTNSVRLKELPFDSDRKMMTVIQSADTNHQFNTYTKGAPNVVVAHCDYYLINGEVKPLGDDIRQQILAANDAYAKQGLRVLAVAGRTLPDNLYQDLSQATNDTVEQALVFIGLSVMLDPPRKEVVAAAKLCHQAHIKIIMVTGDYSLTAESIAKKIGLVDPNQPLRVVTGEELGAMTDDDLKAALSGEIVFARMAPEQKYRVVNALQEMGEVVAVTGDGVNDAPALKKADIGVAMGVTGTDVAKEAADMILTDDNFASIVEAIREGRGVYANIRKFLLYILNSNMPEAIPSVLFLLSGGKIPLALTVMQILAIDLGTDMIPALGLGKQRVEAGIMERPPRKMSEHLINRQLVLKAFGWYGLLSSIISVIAFFYGNLMHGYSLANLPTSGNVYVQATTITLGAIIFCQIAAVLNIRYNQQTMFTRQFWDNSMITIGIIFEIALFLCLSYVPVLQSVFQTAPLNAHNWYFLMAIPIPLILIDEFRKWILRRQHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJG4</accession>
    <name>H4GJG4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_20233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000038">
        <property type="protein sequence ID" value="EHS86718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS86718">
        <property type="protein sequence ID" value="EHS86718"/>
        <property type="gene ID" value="PS3_20233"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="203" mass="23358" checksum="99B57FC2B0F5E933" modified="2012-04-18" version="1" fragment="single">MADKLKIKGSGIAILALYSAIALLFIFPVLHGKSLIYGGDMLYHLRRINELSIDIKHGNLYPYLYAYDFKNFLFPGGIFYPQITLLPFSILKVFFGGYFIPIIFGWWFYTLITLLNTHLVVYRYSGNLLQAIFSSVIYSFSTYRAIDVWHRFALGEALSMCFLPLVIYGIYSIVYGNDREWPFLSIGLGFTIFSHVLSTYLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJH5</accession>
    <name>H4GJH5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Arginine repressor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">argR</name>
        <name evidence="10" type="ORF">PS3_13411</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Regulates arginine biosynthesis genes.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-arginine biosynthesis [regulation].</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ArgR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000039">
        <property type="protein sequence ID" value="EHS86618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122208.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000039.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13411"/>
    <dbReference type="EnsemblBacteria" id="EHS86618">
        <property type="protein sequence ID" value="EHS86618"/>
        <property type="gene ID" value="PS3_13411"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.929"/>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034618">
        <property type="term" value="F:arginine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051259">
        <property type="term" value="P:protein complex oligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00173">
        <property type="entry name" value="Arg_repressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001669">
        <property type="entry name" value="Arg_repress"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020899">
        <property type="entry name" value="Arg_repress_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036251">
        <property type="entry name" value="Arg_repress_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020900">
        <property type="entry name" value="Arg_repress_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34471">
        <property type="entry name" value="PTHR34471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01316">
        <property type="entry name" value="Arg_repressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02863">
        <property type="entry name" value="Arg_repressor_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01467">
        <property type="entry name" value="ARGREPRESSOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55252">
        <property type="entry name" value="SSF55252"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Arg_repressor" evidence="8">
        <location>
            <begin position="4"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Arg_repressor_C" evidence="9">
        <location>
            <begin position="81"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS86618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="151" mass="17048" checksum="6672E42FD33FEC7C" modified="2012-04-18" version="1">MDFRTRRALIKDLIKEREISTQEELMELVAQHGVQATQATISRDLQHLNIVKVMGQQGRTYYAQLQETPMALNERLVEAIRHRVESVTTVQFMNVVKTTPNSNFAMIVAGEFDGDENEKIVGTLAGNDTLIIISPDEEAARAVAEFINQYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJH6</accession>
    <name>H4GJH6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_4299</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000039">
        <property type="protein sequence ID" value="EHS86619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122209.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000039.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4299"/>
    <dbReference type="EnsemblBacteria" id="EHS86619">
        <property type="protein sequence ID" value="EHS86619"/>
        <property type="gene ID" value="PS3_4299"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.930"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="11"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="172" mass="19582" checksum="64709C0E5CACAA75" modified="2012-04-18" version="1">MPTETFLNLDPNKQSRIIQACLHEFGTRPLSEAQVAPIVKEAKIARGSFYKYFEDLTDAYQYVYGIALREIHKGIRPPDRGHGQVSDYLAQVTNFLDQSHQSGYYDLIRQHLLHNEERIPPRPQAVPMELSPQNWAVGVLVHTTIKECVREPKAQAAKLERLAAVLTKLLAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJJ0</accession>
    <name>H4GJJ0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_22232</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_22232"/>
    <dbReference type="EnsemblBacteria" id="EHS86500">
        <property type="protein sequence ID" value="EHS86500"/>
        <property type="gene ID" value="PS3_22232"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.950"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013663">
        <property type="entry name" value="Helicase_SWF/SNF/SWI_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08455">
        <property type="entry name" value="SNF2_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="186"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="467"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="635" mass="72033" checksum="A683B45C2BD84901" modified="2012-04-18" version="1">MPLEEDQQVVAFLNQGLKRLEDFGEVQVTKPFQRLIGQIDHKPNLTVGISLESGLLNLSVAGEAMDSADIAQALRAYHERRPYFKLKNGDYMGVESTTLTTLAHTLEDLGVDADQVDQGDISLPAYQALALNQSLQEQNDLTYQTDDHYAKLIRDFDQNQVGSFTVPQDLQAKLRPYQIAGFQWLMTLNHYHFGGLLADEMGLGKTIQIITLLLAINQEQKLPSLVVAPASVVYNWQDEFHKFAPSLKIATLGGGKVERRKLLAKSDQYDVFITSYDSLKRDIALYDQLEFQVQIIDEAQTIKNARTIGAQTVKLIHAQQRFALTGTPIENRLSELWSIFDYLMPGFLGTYQHFSKRFEEPIIFNDDQAKRAQLSQLIGPFVLRRLKKDVLTSLPAKTEIVEYAEMSGVQLKLYRARADQLIKRIQTSDETDFKGDKIEILAELTRLRQVCCDPHLVYDDYKGKSAKLSQTIALIKDQIENGHKILLFSQFTSMLDIIAQRLQKERIQTETITGAVDKQERQRRVKQFNEQDEPAVFLISLKAGGTGINLTSADVVIHYDPWWNAAAQNQATDRAHRIGQENPVTIYQMITKHTIEERIKELQDKKQALADQVLSGDQMSTTSFDKDELLAILDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJJ8</accession>
    <name>H4GJJ8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_20013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122231.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20013"/>
    <dbReference type="EnsemblBacteria" id="EHS86508">
        <property type="protein sequence ID" value="EHS86508"/>
        <property type="gene ID" value="PS3_20013"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.969"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="PTHR18964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="300" mass="32295" checksum="1361661ECEA8D316" modified="2012-04-18" version="1">MNYLSIDVGGTNLKYALISAAGQIIEKDKIKTPQDRENFLQALDQIVEQYHDQIKGLAFCAPGQIEKTTIHFGGALPFLDGLDLKRHFHELTIPIIGLNDGKAAALAENWLGSLKGEQNCAAIVLGTGVGSGIIVNGHLVAGSHYQAGELSFVNLNYHASDFNDAFVGGIGSAVEMIKRINQVVGNDDETDGLKAFQAIEDGNAAAQAILNDYCYGIATLIMDVQFVVDLDKYAIGGGISAQSKVIQGIRQAYQAIFAKYPIIQQTFQQPEIVAAKFNNDANLYGALYNLLLTVNGETLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJL7</accession>
    <name>H4GJL7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_22153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122250.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_22153"/>
    <dbReference type="EnsemblBacteria" id="EHS86527">
        <property type="protein sequence ID" value="EHS86527"/>
        <property type="gene ID" value="PS3_22153"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.995"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010662">
        <property type="entry name" value="Hydrolase_RBBP9/YdeN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06821">
        <property type="entry name" value="Ser_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="180" mass="20163" checksum="89731BC6ECF957F1" modified="2012-04-18" version="1">MQVGYLIHGTSTRDDDWFPWLEASLKPQVNLERLWLPQPFDPDPLEWERAMEEQMNPVAGTLLVAHSLGCLATLRYIEQNTVKDLRLVLVGAFDQPLPAYPQLEAFCQPPLDYAQIKTKVSAATIITARDDYIAPADLAVQVAQRLAAKLIELSTGGHFLTSDGYQQFPLVKAECLKLID</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJL9</accession>
    <name>H4GJL9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_17962</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122252.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17962"/>
    <dbReference type="EnsemblBacteria" id="EHS86529">
        <property type="protein sequence ID" value="EHS86529"/>
        <property type="gene ID" value="PS3_17962"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.997"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="212" mass="23771" checksum="96289D610060CC90" modified="2012-04-18" version="1">MITNFIFDIDGTLIDTSEMYMPAMIMALADFGYHYSPEEETKYQKELFGITGHDSLVQLGIPEDQLSEILTRWNEYTNQRKAYMRSLPGIAEALDQLAQRPQTQLAVATSKKAPEYADFTQRFAFAKYFKVVIKEEDTRKHKPDPEPLLAAMQGLAGNPENTIYVGDTINDLKAAHAAGMKFAGALYGSSQPEKIQSADFLLHQPADLLTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJN2</accession>
    <name>H4GJN2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CRISPR-associated endoribonuclease Cas2</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cas2</name>
        <name evidence="10" type="ORF">PS3_13735</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, forms a heterotetramer with a Cas1 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CRISPR-associated endoribonuclease Cas2 protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000044">
        <property type="protein sequence ID" value="EHS86255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122265.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000044.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13735"/>
    <dbReference type="EnsemblBacteria" id="EHS86255">
        <property type="protein sequence ID" value="EHS86255"/>
        <property type="gene ID" value="PS3_13735"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1021"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01471">
        <property type="entry name" value="Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021127">
        <property type="entry name" value="CRISPR_associated_Cas2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019199">
        <property type="entry name" value="Virulence_VapD/CRISPR_Cas2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09827">
        <property type="entry name" value="CRISPR_Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01573">
        <property type="entry name" value="cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS86255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="101" mass="12067" checksum="D1B2368F19381DCF" modified="2012-04-18" version="1">MRLMVMFDLPVETSQQRRAYRVFRKKLIQEGFLMIQYSVYVRVCVNRKAAQFMEKRISNFLPENGLVQTLMLTEKQYNDMHFLLGEEKDEVRNSSARTVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJQ3</accession>
    <name>H4GJQ3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative galactoside O-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_8382</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000047">
        <property type="protein sequence ID" value="EHS86203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122286.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000047.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8382"/>
    <dbReference type="EnsemblBacteria" id="EHS86203">
        <property type="protein sequence ID" value="EHS86203"/>
        <property type="gene ID" value="PS3_8382"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1049"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="171" mass="18285" checksum="45E70FED6CD9FF1A" modified="2012-04-18" version="1">MRLSAEQESRIQANIRAIQKLNNSSLSDAEIRQNLSAIIQSPIDETNTIRLPLFVDSGVNIHFGKHDFINVGVTLVDLGGIYFGDQVLVAPQARILTVNHPFLDPQIRQAGDLELHAVHLKDNCWIGAGATILPGVTVGTNAIVAAGAVVSHDVPDNAVVAGLPAKVIKYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJQ4</accession>
    <name>H4GJQ4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_13624</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000047">
        <property type="protein sequence ID" value="EHS86204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13624"/>
    <dbReference type="EnsemblBacteria" id="EHS86204">
        <property type="protein sequence ID" value="EHS86204"/>
        <property type="gene ID" value="PS3_13624"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1050"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="3"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="243" mass="27870" checksum="13B125888F39125F" modified="2012-04-18" version="1">MFRDLMPALADHFHLIAPDYPGFGQSQSPDRDHFNYTFDHLAQVMDHFLDQLKINQFLMYVFDYGAPIGFRLASWHPERIAGIISQNGNVYEEGLGPKWTQRAEYWQHPTPELRRQYQSAFAKETVIGQYTYGTPAGSVSPDGYSLDLYYAATIDDYAEKQSDLILDYQSNVRLYPQFQAYLRQYQPKLLAIWGKEDPSFIWAGAEAFKKDLPAARIIPVASGHFALENCCSQIATEIIKFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJR3</accession>
    <name>H4GJR3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC superfamily ATP binding cassette transporter, membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_11206</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000047">
        <property type="protein sequence ID" value="EHS86213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122296.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000047.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11206"/>
    <dbReference type="EnsemblBacteria" id="EHS86213">
        <property type="protein sequence ID" value="EHS86213"/>
        <property type="gene ID" value="PS3_11206"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1069"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="7">
        <location>
            <begin position="56"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="353" mass="38604" checksum="4CFCF5CFC7F701A1" modified="2012-04-18" version="1">MFLALHEMKHEKLRYGMVIFMIMLISYLVFVLAALALGLANQEYQALDTWNVKSAVMTTTSDKELRQSTLTPQQVKKLTKNQDNAVVAINSVIAKASGKKQVASTFVGIDKDQFIYHDLKLTSGHKPTNSHQVVVNESYQNDGYKLGDQIKLGSDQTKYRIVGFAKNSMLQIAPVIYGTVSTWQQIRRTGDLFAGSAVVSKKSSIKVNESGLKSYPIQQVIEKLPGYSAQNMTFEFMIGFLLIISLIVIAVFLYIITIQKLSNYAVLRAQGVPSRFLVNATIAQSILLTVSGLVIAVLLVWGTSLLMPAAVPMSFNWGLVGAISLGLVVMSLLGSIIPVRVVTKVDPVSVIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJR6</accession>
    <name>H4GJR6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_19383</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPF0324 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000047">
        <property type="protein sequence ID" value="EHS86216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122299.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000047.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS86216">
        <property type="protein sequence ID" value="EHS86216"/>
        <property type="gene ID" value="PS3_19383"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1072"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018383">
        <property type="entry name" value="UPF0324_pro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30106">
        <property type="entry name" value="PTHR30106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03601">
        <property type="entry name" value="Cons_hypoth698"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS86216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="343" mass="36331" checksum="18351A5BDC1AB831" modified="2012-04-18" version="1">MERFRNNLLGIVVCLIIAIPAWLLGKVFPVIGGPVIGIIAGMIITLFWTNKGYAAAGIGWTSKVILQTAVVLLGFGMDFGVIIQTGGQSLPIIVCTIGIALIIAWVLHKWLRIPSKIATLVGVGSSICGGSAIAATAPVIDADDDEVAQAISVIFFFNVIAAVLFPVLGHLLGFATNSGNAFGIFAGTAINDTSSVTAAASTWDSMWNLGSQTLDKAVTVKLTRTLAIIPITLILALMNTKQADGEAKNHFSLKRAFPMFILYFILAAIFTTICIHLGVAATAFNPLKELAKFMIVLAMTAVGLNSNVVKLIKTGDKPLFLGATCWVGITLVSLLMQRVMGIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJT4</accession>
    <name>H4GJT4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Signal recognition particle protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Fifty-four homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ffh</name>
        <name evidence="9" type="ORF">PS3_21921</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="7">The SRP-RNC complex is targeted to the cytoplasmic membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="7">Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122317.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21921"/>
    <dbReference type="EnsemblBacteria" id="EHS86121">
        <property type="protein sequence ID" value="EHS86121"/>
        <property type="gene ID" value="PS3_21921"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1111"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004780">
        <property type="entry name" value="SRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="PTHR11564"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF7">
        <property type="entry name" value="PTHR11564:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="SSF47446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00959">
        <property type="entry name" value="ffh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54" evidence="8">
        <location>
            <begin position="270"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="108"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="191"/>
            <end position="195"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="249"/>
            <end position="252"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS86121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="483" mass="54069" checksum="0007A9C4CE048CB2" modified="2012-04-18" version="1">MAFEGLTERLQKTMEKLRRKPKVTDADLRETMREIRLALLEADVNFTVVKNFVKTVQEQAVGTEVLKGLNPAQQIVKIVNEELTKMMGETAVPLNKSDHIPTVIMMVGLQGAGKTTTAGKLALRLKNEEKARPMFIAADIYRPAAITQLKQVADSIDVPVFDLGTDVDPVEIVRQGMEVAKEHHNDYVIIDTAGRLQIDEALMDELANIKALAHPDEILLVVDAMTGQNAVNTAEGFDEKLDITGVVLTKLDGDTRGGAAMSIRAVTGKPIKFVGEGEKMENLDVFYPDRMSSRILGMGDMLTLIEKAQKNFDEEQAAETMRKMQENNFDYNDFLDQMDQVSKMGPLEDLIKMMPGMANNPALKNINIDPKQFLHIKAIIQSMTPEERENPDLMNPSRRRRLAAGSGRPIVEVNRMIKQFDQMRKMMKQVTNGNLNGMENMMGGLGNMPGMPGGKLGKMAMNRMARQMKKNKKKRLKKNKKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJX2</accession>
    <name>H4GJX2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_20284</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 8">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS85989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122355.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20284"/>
    <dbReference type="EnsemblBacteria" id="EHS85989">
        <property type="protein sequence ID" value="EHS85989"/>
        <property type="gene ID" value="PS3_20284"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1161"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05313">
        <property type="entry name" value="NAD_bind_2_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELFV_dehydrog" evidence="9">
        <location>
            <begin position="202"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="376"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="7">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="445" mass="48481" checksum="67F16B02DB0DA88A" modified="2012-04-18" version="1">MSYVDDVYERLVAQNPAQPEFHQAAKEVLDSLRPVIEPNEEHYRKEALLERLTTPDRQILFKVPWVDDNGQVQVNNGFRIQFNNAIGPYKGGLRLHPTVNLGILKFLGFEQIFKNALTTLPIGGGKGGSDFDPKGKSDREVMAFCQSFMTELYKHIGPDVDVPAGDIGTGGREIGYLYGQYKRLTTSYQGVLTGKGLNWGGSLARTEATGYGLLYIVDELLKDHGQSLEGKTVTVSGAGNVAIYAIEKAQQLGAKVVTASDSTGWVYDPEGIDVALLKDVKENRRARLTAYAEERPSAEYHEGRGVWVVKADVALPCATQNELTLDDAKTLVENGTVAVAEGANMPTTPEATEYLQEKGVFFVPGKAANAGGVAVSALEMSQNSERLSWTFEEVDNKLHDIMKDIYQNISSAAEKYAKKDDFVSGANIAGFLKVADAMEAQGTAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJX8</accession>
    <name>H4GJX8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nitroreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_10203</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the flavin oxidoreductase frp family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS85995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122361.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10203"/>
    <dbReference type="EnsemblBacteria" id="EHS85995">
        <property type="protein sequence ID" value="EHS85995"/>
        <property type="gene ID" value="PS3_10203"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1174"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02146">
        <property type="entry name" value="NfsA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016446">
        <property type="entry name" value="Flavin_OxRdtase_Frp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43425">
        <property type="entry name" value="PTHR43425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005426">
        <property type="entry name" value="Frp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="4">
        <location>
            <begin position="13"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR005426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="253" mass="28591" checksum="80319A07EE4C1D05" modified="2012-04-18" version="1">MIENETIKHQLNHRSIRQFKPTTLSAEQLTTLYEVARQTSTSMFMQQMTILHLTDEKKRAAVREICHQPYVGANGDLLIFIADLHRNQLIRQQAGNNDGRLHTTDIFLQAVEDTVLATQNVLNAVESMGLGGVVLGSIKNDSQKLIEVLGLPKMAFPILGLQIGIPDQDPQLKPRLPLSLMAFENDYPKTMPMDLMPDYDQVVNTYYDLRNANQRVDTFTNQINSDKLNQQVTKRDEIVAALHQQGLALDLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK47</accession>
    <name>H4GK47_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Competence protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_7296</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GSP F family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000056">
        <property type="protein sequence ID" value="EHS85814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122430.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000056.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7296"/>
    <dbReference type="EnsemblBacteria" id="EHS85814">
        <property type="protein sequence ID" value="EHS85814"/>
        <property type="gene ID" value="PS3_7296"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1287"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003004">
        <property type="entry name" value="GspF/PilC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30012">
        <property type="entry name" value="PTHR30012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="8">
        <location>
            <begin position="24"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="8">
        <location>
            <begin position="207"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS85814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="337" mass="39350" checksum="A8AC794238A1C8E0" modified="2012-04-18" version="1">MKLTSNLKPARFTLSHQAQWFVFLGDALNSGMNLPQALAFSGRIFPKELDRFQKMERQLVQGGGLAETMKPWLRTDLYYQLKLAEQEGNLAGVVRQIGEFLTKQHQQRQKLRGLLQYPLLLLTVLGVFMMIMVHYVGPQLSSWQQGDWGIKPRILQILVMTVSAIMTILIWQGIVLLKMSRLERINWLCHLPVVGNYWRLYWHYYLVNVLGMMIAHGMSFQDIHRVTRQYDHQSMVYQVIQQLTRQTQRGQSIERVILKTIYLPDELASYFNRGLTTSELGDELLIYSRMLFQRFINQTERLLELVQPILFMLIAMIIGGMYLSLLLPIYHSLQSVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK57</accession>
    <name>H4GK57_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DAGKc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_21930</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the diacylglycerol/lipid kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000059">
        <property type="protein sequence ID" value="EHS85691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122440.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000059.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21930"/>
    <dbReference type="EnsemblBacteria" id="EHS85691">
        <property type="protein sequence ID" value="EHS85691"/>
        <property type="gene ID" value="PS3_21930"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1302"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005218">
        <property type="entry name" value="Diacylglycerol/lipid_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045540">
        <property type="entry name" value="YegS/DAGK_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19279">
        <property type="entry name" value="YegS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00147">
        <property type="entry name" value="TIGR00147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAGKc" evidence="2">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="338" mass="37175" checksum="BB90BBEDEBFF03F3" modified="2012-04-18" version="1">MTKRARVIYNPTSGREAIKQDLVEILAVYEQAGYETSAYATTPEPNSAKMEASRAVREGFDLIVAAGGDGTINEVMNGIAPFEQRPLLAVIPSGTTNDYARALKIPREDPVGAARVILNPENRFKIDVGQAGDNYFMNIAAAGTLTELTYDVPSQMKSLFGYLAYFAKGAELLPRVKAVDVRVKYDDQVYEGEVSTIFLALTNSVGGFEQVVPDASLDDGNFSLLIVKDSSIPGMLQLMAKALKGNHVGDDRIIYAKAKDVFVEPVHEEDRLMINLDGEYGGDAPMQFHNLRQHINVVANVAEIPNEAISSEMTPELQRMEKDFIRDVDKVHEVESNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK58</accession>
    <name>H4GK58_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B</fullName>
            <shortName evidence="10">Asp/Glu-ADT subunit B</shortName>
            <ecNumber evidence="10">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gatB</name>
        <name evidence="12" type="ORF">PS3_13424</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 10">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="10"/>
    <dbReference type="EMBL" id="AICN01000059">
        <property type="protein sequence ID" value="EHS85692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122441.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000059.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13424"/>
    <dbReference type="EnsemblBacteria" id="EHS85692">
        <property type="protein sequence ID" value="EHS85692"/>
        <property type="gene ID" value="PS3_13424"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1303"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="Apn/Gln-ADT_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="PTHR11659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GatB_Yqey" evidence="11">
        <location>
            <begin position="325"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS85692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="474" mass="52723" checksum="537EC8764B375572" modified="2012-04-18" version="1">MNFETTIGLEVHVELKTNSKIFSPSPVNFGAEPNANTNPLDWGYPGVLPTLNKGVVKAGIMAGLALHAEITKDMHFDRKNYFYPDNPKAYQITQSETPIAHDGWVEIEVDGNKKRIGIAEMHIEEDAGKNTHTGQYSYVDLNRQGTPLIEIVSKADIASPDEAVAYLEALRQRIQFTGISDVKMEEGSMRVDTNISVRPIGSDQYGTKTEMKNINSFNYVRNALNFEQERHQKVLLSGGQIVQETRRYDEATKSTISMRTKEGSDDYRYFPEPDVPAVHVSDEWIDQINAEMPQMPGERREHYVNDLGLTDYDAMVLTQTKEMSDFFEGTVAEGADARKVANYLMNDVNSYLNTEHVDLPATKLTPANLAGMIKLIDDGTISSKMAKKVFKGILDGQEPAAYAEKNGLVQLSDPAQLQPIVDEVLANNQQSIEDFKNGKDRAVGFLVGQIMKQTKGKANPGVVNKLLMQALNAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKA3</accession>
    <name>H4GKA3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">S-ribosylhomocysteine lyase</fullName>
            <ecNumber evidence="4 14">4.4.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">AI-2 synthesis protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 14">Autoinducer-2 production protein LuxS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">luxS</name>
        <name evidence="15" type="ORF">PS3_12188</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 14">Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = (S)-4,5-dihydroxypentane-2,3-dione + L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:17753"/>
            <dbReference type="ChEBI" id="CHEBI:29484"/>
            <dbReference type="ChEBI" id="CHEBI:58195"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="4.4.1.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="14">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 14">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the LuxS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.21" evidence="4 14"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122486.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12188"/>
    <dbReference type="EnsemblBacteria" id="EHS85555">
        <property type="protein sequence ID" value="EHS85555"/>
        <property type="gene ID" value="PS3_12188"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1370"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043768">
        <property type="term" value="F:S-ribosylhomocysteine lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009372">
        <property type="term" value="P:quorum sensing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00091">
        <property type="entry name" value="LuxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037005">
        <property type="entry name" value="LuxS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003815">
        <property type="entry name" value="S-ribosylhomocysteinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35799">
        <property type="entry name" value="PTHR35799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02664">
        <property type="entry name" value="LuxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006160">
        <property type="entry name" value="AI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01487">
        <property type="entry name" value="LUXSPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0071">Autoinducer synthesis</keyword>
    <keyword evidence="9 14" id="KW-0408">Iron</keyword>
    <keyword evidence="10 14 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0673">Quorum sensing</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EHS85555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="162" mass="17879" checksum="6E5981A480FF2C9B" modified="2012-04-18" version="1">MAKIETEVESFELDHTKVKAPYVRLITVEEGPKGDKISNFDLRLTQPNETAIPTGGLHTIEHLLAGLLRDRMDGVIDCSPFGCRTGFHLIMWGEHSTTEVAKALKSSLAAIANDIEWSDVPGTTIESCGNYRDHSLFSAKEWSKAILAQGISDDPFTRHVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKA5</accession>
    <name>H4GKA5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_2980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122488.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2980"/>
    <dbReference type="EnsemblBacteria" id="EHS85557">
        <property type="protein sequence ID" value="EHS85557"/>
        <property type="gene ID" value="PS3_2980"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1372"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="173" mass="19515" checksum="1BEE5F644AE05DF7" modified="2012-04-18" version="1">MTETYIRLAQPADLATIMGIIEDARQTLKAAGSTQWQGEYPNQSTINDDLAAHHAWVLVHQEQVVGYAAMVSGPDPSYEKIDGSWKINDQPYVAIHRIAVAKSHQGKHLVTPFMQLLIRESLNEGILDYRIDTGLNNVVMQHLITKLGFQQRGYVKVPTDLIEPRRVAYELNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKB6</accession>
    <name>H4GKB6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase_S9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_19134</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122499.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19134"/>
    <dbReference type="EnsemblBacteria" id="EHS85568">
        <property type="protein sequence ID" value="EHS85568"/>
        <property type="gene ID" value="PS3_19134"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1387"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S9" evidence="2">
        <location>
            <begin position="107"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="291" mass="32879" checksum="4FE7DF5CF71FB6AE" modified="2012-04-18" version="1">MPIKELTNNPTWAGQLRLIERVAYACADGEPQYLSILAPWVQRYPTTRTTPRPLIVFVQGSSWRLPTLGEQIPQLVDFAKQGYIVATVQHRNSLDGHPFPAFLEDVKTAIRYLRAHADEYMIDPNRVAIWGTSSGANTALLVGLTGNDQRYQTQDYPDQSDAVSAVVSCFAPTDVQDTFQYTAMVPGNDVLQYALFGPDQSKWDELKIAMSPVHQIKAGVIYPPFLLFHGDQDKTVPYKEMETMYQALVDQGALVEAYRVKGADHERDFWSAAVYETVLEFLNRYEQEGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKC2</accession>
    <name>H4GKC2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">GPH family glycoside-pentoside-hexuronide:cation symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_15227</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122505.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15227"/>
    <dbReference type="EnsemblBacteria" id="EHS85574">
        <property type="protein sequence ID" value="EHS85574"/>
        <property type="gene ID" value="PS3_15227"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1393"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039672">
        <property type="entry name" value="MFS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001927">
        <property type="entry name" value="Na/Gal_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018043">
        <property type="entry name" value="Na/Gal_symport_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11328">
        <property type="entry name" value="PTHR11328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00792">
        <property type="entry name" value="gph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00872">
        <property type="entry name" value="NA_GALACTOSIDE_SYMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="249"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="338"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="394"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="423"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS85574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="469" mass="52188" checksum="D45A851988E5E1C6" modified="2012-04-18" version="1">MKQHKTLGARLAYTSGAFGNDVFYATLSTYFIMFVTTHLFNTGNQALNAKMVVMMSNAILAMRIIEIFLDPMIGNAIDRTESRWGKFRPWVVIGGVVSSLLLLVLFTNMGGLNQSPVLYFIAFVVVYFVMNVFYSFKDTAFWSMIPAMSLDSREREKTATFARVGSTVGTNLVGVVIMPIILFFSVHSNKGTGDQQGWFWFVAIVAVIGIVTAIAVGLWTHEVQSKLRETKEKTTLRQVTKVLFNNDQLMWLAFAYLFYGLGINTVNALQLYYFSFILGDSGKYSILYAVNTVVGLISVTLFPSLAKKFNRKNLFVYCMLCMLLGVALYTVAGANFALTLIAAELFFIPQPLTFLVVLMIISDAVEYGQYKSGHRDEALTLSVRPLVDKFGGAISNWFVSMVAVMAGMTTGATASSITAHDQLIFKICMFGIPAIMMVISLIIFMKKVDLTEDMHAEILAKLEAKMKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKD1</accession>
    <name>H4GKD1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aspartate racemase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_14458</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartate/glutamate racemases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122514.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14458"/>
    <dbReference type="EnsemblBacteria" id="EHS85583">
        <property type="protein sequence ID" value="EHS85583"/>
        <property type="gene ID" value="PS3_14458"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1402"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047661">
        <property type="term" value="F:amino-acid racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015942">
        <property type="entry name" value="Asp/Glu/hydantoin_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001920">
        <property type="entry name" value="Asp/Glu_race"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018187">
        <property type="entry name" value="Asp/Glu_racemase_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004380">
        <property type="entry name" value="Asp_race"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01177">
        <property type="entry name" value="Asp_Glu_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53681">
        <property type="entry name" value="SSF53681"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00035">
        <property type="entry name" value="asp_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00923">
        <property type="entry name" value="ASP_GLU_RACEMASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="237" mass="26748" checksum="560B34530D447913" modified="2012-04-18" version="1">MQKFFTVLGGMGTLATESFVRVMDQRTPIHCDQDYLDYLVVNHATVPDRTNWILDHRQPSIVPPLLEDIQQQSLLKPSFFVLACNTAHYVYDQLQAATEIPIINMLAETVAEVSKIMPQAKRVGILATPGTIQSGLYDPYIKKAGYEVVRPTKEILALTEDLIYNDIKEAGHSDNQKYHQLVKQMIEDLDCDIVILGCTELSYAEEMDPETTYPVADSQSIVVDRTIEKALSIRQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKD3</accession>
    <name>H4GKD3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Dipeptide/tripeptide transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PS3_3354</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122516.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3354"/>
    <dbReference type="EnsemblBacteria" id="EHS85585">
        <property type="protein sequence ID" value="EHS85585"/>
        <property type="gene ID" value="PS3_3354"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1405"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006857">
        <property type="term" value="P:oligopeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17346">
        <property type="entry name" value="MFS_DtpA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005279">
        <property type="entry name" value="Dipep/tripep_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="PTHR11654"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00924">
        <property type="entry name" value="yjdL_sub1_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01022">
        <property type="entry name" value="PTR2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="98"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="230"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="292"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="10">
        <location>
            <begin position="1"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS85585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="496" mass="54859" checksum="5CF3966AACAA6391" modified="2012-04-18" version="1">MQKDYDHAFFGQPRGLSTLFFTEMWERFSYYGMRAILLYYMYFAVTKGGLGMDQTLAASIMSIYGSLVYLSGVVGGWISDRILGSQRTVFIGGILIMFGHIALAIPAGVSALYISILLIVMGTGLLKPNVSEMVGDLYTEDDQRRDAGFSMFVFGINLGAAIAPILVPWAADGFGMHLFGNQVNYHAGFALAAVGMFFGLLQYWLDGRKYLSKDSLYPDDPIDKESLKPVIIWTVIGLVIVALVLALLAAMSLFNIKSIINLITFVAICLPIVYFIMMINSRKVTKVERSRVIAYVPLFIAAAIFWGIEESGSVVLALFAQERTILHLGNWHFEAANFQTLNPLFIMLLTPVFVALWNRWKKQPSAPGKFAYGLIFAGLSYVWMALPGMINGTSGKVSPLWLVGSWFIVEIAEMLISPIGLSVTTKLAPKAFSGQMMSMWFLANSAGQAVNAQIVQFYSSKTEVQYFLVIGGVSIIFGIILFFFVKQIHALMEGID</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKD4</accession>
    <name>H4GKD4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phage antirepressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_18895</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000063">
        <property type="protein sequence ID" value="EHS85498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122517.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000063.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS85498">
        <property type="protein sequence ID" value="EHS85498"/>
        <property type="gene ID" value="PS3_18895"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1415"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014054">
        <property type="entry name" value="Phage_regulatory_Rha"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09669">
        <property type="entry name" value="Phage_pRha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS85498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="245" mass="28657" checksum="623DF8486819B8CA" modified="2012-04-18" version="1">MDTQVQIIKQVNDQDVMDSRDVAKMINKTHAHLMRDIDRYINDLATDPKLDSLDFFIESSYEDAKGETRKCYLLTKQGCEFVANKLTGKKGTIFTATYVGLFNQYQEEHNGKLIGGKDKKLTRDNLEFKREWLLEMRRQNDNKEHQLRNEDAKIWLELADRADEYGEARMALDMRNDVIGMMTRLPISGQEEYSASQIGKWGSILGIKSDRHLSYKTAEGAWRYYPKAVELFQKNQFKIQEDFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKD9</accession>
    <name>H4GKD9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YrdC-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_22124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000063">
        <property type="protein sequence ID" value="EHS85503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122522.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000063.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_22124"/>
    <dbReference type="EnsemblBacteria" id="EHS85503">
        <property type="protein sequence ID" value="EHS85503"/>
        <property type="gene ID" value="PS3_22124"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1432"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YrdC-like" evidence="1">
        <location>
            <begin position="9"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="257" mass="29232" checksum="4F054B737330F5BC" modified="2012-04-18" version="1">MTKHVQWQGQIDQEVYNALNQPGSMIVSPTKVGYIIMTNDKAGLERKFDAKQRNRNKPGVVLCGSMEELKELAQLTPSIEALYQRHWDEDILLGCILPWQPEAIEKYLQDDDRQELMMDRRQTSCFVIKFGTPSEQMAKAMWEQEGKMVYASSANPSGKGNRGKVAGIGERIESKADLILDADDYVESIQPDKTIDTRYEQGVMVSMVDEAGKIINDQPEAGQACPVVIRKGLDIDKIMMNLADQYDVWDYRHGQYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKG7</accession>
    <name>H4GKG7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Intracellular protease/amidase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_15702</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000067">
        <property type="protein sequence ID" value="EHS85381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122549.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000067.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15702"/>
    <dbReference type="EnsemblBacteria" id="EHS85381">
        <property type="protein sequence ID" value="EHS85381"/>
        <property type="gene ID" value="PS3_15702"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1492"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1_PfpI" evidence="1">
        <location>
            <begin position="26"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="225" mass="24554" checksum="8E72EC1E9271056F" modified="2012-04-18" version="1">MTKALIVLTNVAKYDSLDRPTGLWLSELTHFYDVMRDHQVEVDFVSPTGGYIPLDPHSMMSMDATDWKYYQDPDFRNQVLGATLSPDQVVPQDYDLIYYAGGHGAMWDFPASTAIADIASQIYQNNGLVAAICHGVAGLLPIKDEKGEPLVADKLLTGFSNVEEAANETTDAVPFLAEDALKELGANYQAGPSFTSCVQVDGRLLTGQNPQSARDLAEKVIEHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKI1</accession>
    <name>H4GKI1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Capsular polysaccharide biosynthesis protein CpsC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_10549</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122563.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10549"/>
    <dbReference type="EnsemblBacteria" id="EHS85261">
        <property type="protein sequence ID" value="EHS85261"/>
        <property type="gene ID" value="PS3_10549"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1522"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009103">
        <property type="term" value="P:lipopolysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003856">
        <property type="entry name" value="LPS_length_determ_N_term"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02706">
        <property type="entry name" value="Wzz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Wzz" evidence="8">
        <location>
            <begin position="3"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS85261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="205" mass="22047" checksum="878037DAA32BE4B4" modified="2012-04-18" version="1">MSNKITLNNISKVVWKNLIVILVITIIGGLAGGFYAEHKKHTSYVADRSLMISHGYNDSTANEQVQADLSLTKTYKGMLDSIDVANAARKTLPKKLQKKYSASEIQSMIRVKTVDQSLVLDVSVRADRAKDATKIVNAVVDAAQTELPKMSPSVGTVSTFAKAKTSDATSQTSPSAKKYALLGAAVGLLVGMVLAFSITTWKNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKJ5</accession>
    <name>H4GKJ5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flavodoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_16209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122577.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16209"/>
    <dbReference type="EnsemblBacteria" id="EHS85275">
        <property type="protein sequence ID" value="EHS85275"/>
        <property type="gene ID" value="PS3_16209"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1539"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="148" mass="15939" checksum="3F76F83EEC2D356E" modified="2012-04-18" version="1">MTKALVVFATITGNNEDVADIITSTFEERNINVEMTEITLADAADFADVDICVVCPYTYDEGALPDEGLDFYDDLQETDLSGKVYGVAGSGDTEYGDDFCVAVDKFAEAFETAGATKGADLVKINLSPEGDDVIQLEKMVDQLIAQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKK5</accession>
    <name>H4GKK5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative gluconeogenesis factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_14262</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for morphogenesis under gluconeogenic growth conditions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the gluconeogenesis factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000070">
        <property type="protein sequence ID" value="EHS85232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122587.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000070.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14262"/>
    <dbReference type="EnsemblBacteria" id="EHS85232">
        <property type="protein sequence ID" value="EHS85232"/>
        <property type="gene ID" value="PS3_14262"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1559"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043743">
        <property type="term" value="F:LPPG:FO 2-phospho-L-lactate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00973">
        <property type="entry name" value="Gluconeogen_factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002882">
        <property type="entry name" value="CofD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038136">
        <property type="entry name" value="CofD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010119">
        <property type="entry name" value="Gluconeogen_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30135">
        <property type="entry name" value="PTHR30135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01933">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142338">
        <property type="entry name" value="SSF142338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01826">
        <property type="entry name" value="CofD_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="325" mass="35860" checksum="5A8A357CCC99EFF3" modified="2012-04-18" version="1">MFHKPKVVVIGGGTGLPVVLSGLRDHNVDITAVVTVADDGGSSGIIRDYINVVPPGDIRNAMAALSELSNLELDIFQYRFKSSDRFFAGHAIGNLIISALSEMKGGIFEAVQELTDLMKVNGRIYPVANEPLVLNAEFTDGTQMAGESEITRAHKNIKRVWVTTDGDDHEPQAVPAVIDAILEADQVVIGPGSLFTSILPNLMIKNVQAALKATKAEIIYICNIMTQKGETDGFTDADHVRVLNHHLGYHFIDTILVNSERVPEDYINFQEWNEISSPVGHDFQGLRDQDCRVISSNFLRLRDNGAFHNQNLIVDELINRLHDVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKL9</accession>
    <name>H4GKL9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA threonylcarbamoyladenosine biosynthesis protein TsaE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_15369</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TsaE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122601.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15369"/>
    <dbReference type="EnsemblBacteria" id="EHS85188">
        <property type="protein sequence ID" value="EHS85188"/>
        <property type="gene ID" value="PS3_15369"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1576"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003442">
        <property type="entry name" value="T6A_TsaE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02367">
        <property type="entry name" value="TsaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00150">
        <property type="entry name" value="T6A_YjeE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="156" mass="17018" checksum="A7767DEE23029463" modified="2012-04-18" version="1">MATIGLNNPAQTMALGERLAPLLVAGDVIVLNGDLGAGKTTFTKGIAKGLGIQRVIKSPTFTIIREYLDGRLPLYHMDVYRLEAGGGEDLGLEEYFEGDGISVVEWAEFVADELPSEYLAITFNRCDQAGEEVRSVTLEPHGGHFEQVVKELVEHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKM8</accession>
    <name>H4GKM8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Triosephosphate isomerase</fullName>
            <shortName evidence="4">TIM</shortName>
            <shortName evidence="4">TPI</shortName>
            <ecNumber evidence="4 5">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tpiA</name>
        <name evidence="6" type="ORF">PS3_5728</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="4 5"/>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122610.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5728"/>
    <dbReference type="EnsemblBacteria" id="EHS85197">
        <property type="protein sequence ID" value="EHS85197"/>
        <property type="gene ID" value="PS3_5728"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1586"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="PTHR21139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="SSF51351"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 4 5" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="2 4 5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="3 4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="234"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="active site" description="Electrophile" evidence="4">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS85197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="251" mass="27358" checksum="60C422332FAA8E5A" modified="2012-04-18" version="1">MRTPIVAGNWKMNKTVAESVEFVRQIKTALPPATQLETALAGQPIALTAMLEAAQDSPLKIAAENCYYQSSGPYTGETSPTALYAAGIHHVILGHSERRQLFHEDDQLINRKIKAAITAGICPIVCCDETMGRLITSQRVEWVVARILDDLKGLTAQQAEQVTIAYEPTWAIGSGESADPSQAEEGCYLIRQTLGHMFNETVAQQTRILYGGSITPDNIKQMMAKPDIDGVLVGGASLDPRTFLKLVNFND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKN2</accession>
    <name>H4GKN2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter, CydDC cysteine exporter (CydDC-E) family, permease/ATP-binding protein CydC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_3788</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122614.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3788"/>
    <dbReference type="EnsemblBacteria" id="EHS84952">
        <property type="protein sequence ID" value="EHS84952"/>
        <property type="gene ID" value="PS3_3788"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1592"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034775">
        <property type="term" value="P:glutathione transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014223">
        <property type="entry name" value="ABC_CydC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02868">
        <property type="entry name" value="CydC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="29"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="343"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="377"/>
            <end position="384"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS84952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="582" mass="65606" checksum="09493563CD1B7E6C" modified="2012-04-18" version="1">MHKIPLLRALKNDRWVKPFLTHYKRTLYLAIGLGILTFICAGGLMFVSGYLISKSATHPFNILAVYVPIVITRGFGIFRPVFRYAERLTSHNWVFKMTSEFRKKMYDSLEQDAVFFNSKYRLGDILGLLSEDVAHIQNLYLRTIFPEFVAIGLYLLIVVALGILSWWYGLVMLLLLGVIMVAIPFWSVVVNGAKQEAEKQLKNDLYQDLTDNVMGITDWVFANRGEEYVRQHIADEDKLYRIQESLNRFNQMRTFLIEVWFLLIAISLAFWGVAQFGGHYGGSANWIAAFVLATFPLVEAFAGLPAAAQESNVYVDSIDRLNQLPAVANTKSAPVELTGPYNLEVQDVHYTYPETTKEVLKGVTIDLQAGQKLAILGRSGSGKSTLAALLRGDRKPTHGKITLNGIPTSEFGDEMANYIGVIQQQPYLFHTTLLNNLRIGNEEASEAEIWYVLARVGLKETIAQLPDGLQTMVDEAGLRFSGGERHRLSLARILLKDVPIVILDEPTVGLDPITEQAVIDTFFKQLEGKTIIWITHHLQGIEAMDRVIFIEDGQLEMDGTPQSLMKTNERYRRLKLIDAGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKP1</accession>
    <name>H4GKP1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative gluconate 5-dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_21814</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122623.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21814"/>
    <dbReference type="EnsemblBacteria" id="EHS84961">
        <property type="protein sequence ID" value="EHS84961"/>
        <property type="gene ID" value="PS3_21814"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1606"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS84961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="279" mass="30876" checksum="218B1B9E22A22BFE" modified="2012-04-18" version="1">MDDSWLGLQDKVIVVTGAGGGMGTKFCHDLAQQGARLVLIDLSAERITDLANELTNQYGVQVLPLVVNVTDEHQVDEAVAKTVATFERVDVVVNTAAILHFSPLEDLSLEEWNKELNVNLTGYFLVSQRFGRQMIKQHHGTFVHISTVAAQFPETYSAAYSTTKAGVDMLSKQMAAEWGQFGVRSNCVRPRLVKTPMSAGFYADPKVEQGRSRLVASRRIGNLDDISNTVLFMASDRSDYLNGELINVDGGINVMMQDMIPKPGGRRQYAIDHRYDHKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKQ1</accession>
    <name>H4GKQ1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription termination/antitermination protein NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nusA</name>
        <name evidence="9" type="ORF">PS3_4909</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Participates in both transcription termination and antitermination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer. Binds directly to the core enzyme of the DNA-dependent RNA polymerase and to nascent RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NusA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122633.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4909"/>
    <dbReference type="EnsemblBacteria" id="EHS84971">
        <property type="protein sequence ID" value="EHS84971"/>
        <property type="gene ID" value="PS3_4909"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1622"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1480.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00945_B">
        <property type="entry name" value="NusA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025249">
        <property type="entry name" value="KH_dom_NusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030842">
        <property type="entry name" value="NusA_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036555">
        <property type="entry name" value="NusA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013735">
        <property type="entry name" value="TF_NusA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010213">
        <property type="entry name" value="Tscrpt_termination_fac_NusA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648">
        <property type="entry name" value="PTHR22648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13184">
        <property type="entry name" value="KH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08529">
        <property type="entry name" value="NusA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69705">
        <property type="entry name" value="SSF69705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01953">
        <property type="entry name" value="NusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="5 7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 7" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="142"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS84971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="382" mass="43120" checksum="A4945D298BCCB5C9" modified="2012-04-18" version="1">MAVKKKDRLELLEAMDVLEREKGIKKEIIIDALKTALVNAYQKNYDDSNANVEVVIDENTGAYKLMALKTVVDEVTNPVEEISLAEATLVNRGYEVGDQFRVEVTPRDFGRIAAQTAKNVVLQKLREEERRVVYEKYNRLQDDLINGTVSREDSRYIYVDLGDGVEAAMNIRDRMPNENYRIGDRIEVYVTRVSENTRGPQVFVSRTAPDLLKRLFEKEVPEISEGLVEVKGIVREAGDRAKIAVFSRDEDIDPVGTCVGPRGTRVQTIVNQLGGENIDIVKFEEEPEQFIRNALNPAEVQAVIFDENNGEVEEMGVDEEGNERDPRIHRGCAVIVPDDQLSLAIGKRGQNVRLAAQLTGYKIDIKPYSEVAEDYQEEETAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKT0</accession>
    <name>H4GKT0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein of hypothetical function DUF915 hydrolase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_4366</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122662.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4366"/>
    <dbReference type="EnsemblBacteria" id="EHS85000">
        <property type="protein sequence ID" value="EHS85000"/>
        <property type="gene ID" value="PS3_4366"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1658"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010315">
        <property type="entry name" value="DUF915_hydro-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06028">
        <property type="entry name" value="DUF915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="293" mass="33821" checksum="A01DB7AD66AB1A1F" modified="2012-04-18" version="1">MKWRRLFSDQNNSRKKNRLIITMVSLVLIMLFIPTYFWTKTNHKVMYDRYNSRMSPIIMVPGSSASINRFNSLVSLLNQDTNRKHSLLKIEVENNGKLEYSGKINKNDREPIIVVGFENNHDGYSNIKKQATMLNQAFKSLSTRYHFNNFKAFGHSNGGLIWTRWIETYYADYSDQIELKTLMTVGTPYNFDETSTSRPTQMLSDFIKNRRKIPTDLNVFAIIGTQSFTSDGLVPEASAQAGKYVYQNQAKSYTTLTVSGDNAQHSSLPQNRQIIQMIEQYLMDTNRGTNNSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKT9</accession>
    <name>H4GKT9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyruvate dehydrogenase complex E1 component beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_14614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122671.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14614"/>
    <dbReference type="EnsemblBacteria" id="EHS85009">
        <property type="protein sequence ID" value="EHS85009"/>
        <property type="gene ID" value="PS3_14614"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1667"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transket_pyr" evidence="1">
        <location>
            <begin position="4"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="325" mass="35213" checksum="8DD82D33E569DBE0" modified="2012-04-18" version="1">MAKITMVKAVTDALDEELARDENVEVFGEDVGKNGGVFRATDGLQAKFGEDRVYDTPLAESGIIALANGLATQGLRPVPEIQFSGFIFEAFDEIAGQMARQRFRHSGSRTYPVTIRSPYGGGVNAIELHSDDFSGLLAQIPGLRVVVPSDPYDAKGLLISSIRSDDPVFFLEHLKLYRSFRQEVPDEAYTVPLDKAAVKREGKDVSIITYGYMVRESLAAAEDLAKEGIDVEVLDLRTISPLDEDAILETVKKTGHVVLVQEAPLQGGVSGQVAALIAQQGVLSLDAPIGRVAAPDTPYPVSDAEGVWLPNKQDIIDEVKKTVNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKU7</accession>
    <name>H4GKU7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ion_trans domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PS3_21455</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21455"/>
    <dbReference type="EnsemblBacteria" id="EHS85017">
        <property type="protein sequence ID" value="EHS85017"/>
        <property type="gene ID" value="PS3_21455"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1676"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="PTHR11537"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Ion_trans" evidence="13">
        <location>
            <begin position="16"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="219"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS85017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="261" mass="29786" checksum="28C004F759D90872" modified="2012-04-18" version="1">MKIRKKEQKRLIITYQITMALLAIVSILLIVMDLSRAITITAYPYSLVDNLIWGIFTLDYLIRLGRAKNKKDFFVHNIFDLLSIIPVNLAFSIFRINRLGRLFVVIRLLRLTRLVGLIGRLRYFLKFNGLIYYLYISISVLAITSIMFSIAENRSIWASLWLAITTSTTVGYGDIAPHTVLGKISAVFDMLVGIGLVGVITGNITALFSRRSPRNEHSLKKLHHENQAILKQNADLIKQNQAILNELKTLKDQLQNKQDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKX0</accession>
    <name>H4GKX0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NlpC/P60 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_21051</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C40 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000073">
        <property type="protein sequence ID" value="EHS84944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21051"/>
    <dbReference type="MEROPS" id="C40.007"/>
    <dbReference type="EnsemblBacteria" id="EHS84944">
        <property type="protein sequence ID" value="EHS84944"/>
        <property type="gene ID" value="PS3_21051"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1706"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018337">
        <property type="entry name" value="Cell_wall/Cho-bd_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000064">
        <property type="entry name" value="NLP_P60_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01473">
        <property type="entry name" value="Choline_bind_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00877">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51935">
        <property type="entry name" value="NLPC_P60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003591914" description="NlpC/P60 domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="NlpC/P60" evidence="4">
        <location>
            <begin position="266"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS84944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="384" mass="41477" checksum="6BC292047C65072A" modified="2012-04-18" version="1">MAIATVSVALGTLAVASPVTANADTTTDTTTANQQTTASTSTTDSLSASSVVLASNSATSTSTAASSSAVESTSTAADNVTSASSATSASASSVASDYTGEQVEDGATYYYVNGQKQTDYFYTNDSGQVYYFGDDGKMYQDQFYSNWGNMYYFGEDGARYTDQFYSNWGNLYYFGEDGVRYTDQFYNNWGNTYYFDENGVRYTNQFYSNWGNTYYFDEDGVLYRNKEYDNWGNKYWFDADGVLATNTTVSINGTDYVADANGVLSESGVSAAVAKAMTALGIPYVWGGNTTAGFDCSGLVQWAYGLSSAYRTTYTQQTLGTHVYSNVSAAPYGALLFFGSDSAPYHVAISLGDGTYINAPEPGDVVKINSQKYFAPSYYVVIGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKX3</accession>
    <name>H4GKX3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_5718</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LytR/CpsA/Psr (LCP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000073">
        <property type="protein sequence ID" value="EHS84947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122705.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000073.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5718"/>
    <dbReference type="EnsemblBacteria" id="EHS84947">
        <property type="protein sequence ID" value="EHS84947"/>
        <property type="gene ID" value="PS3_5718"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1710"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004474">
        <property type="entry name" value="LytR_CpsA_psr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03816">
        <property type="entry name" value="LytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00350">
        <property type="entry name" value="lytR_cpsA_psr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="LytR_cpsA_psr" evidence="4">
        <location>
            <begin position="108"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS84947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="404" mass="44831" checksum="96ECA95A125B7A63" modified="2012-04-18" version="1">MNNDFESRSSYRHNRNDQEPAGSNPRPPYHNSNRVYRVIRRVVVIFVLLIAVLGGYEYYQLQQTASSVFSNSAKVSKKLREGKPISILAMGTDEGALGRSESTWGDGNTDSIELITINPKKETITMTSIPRDTLVKVTTSDGDTEYVKINAAYSIGGAKQSVKQIKELLGVPVDYYALVNMSVLQKIVDAVGGVDVNNPFKFTYEGHTFKKGKQHLNGKNALKYSRMRYDDPNNDYGRQKRQQQVIGSIIKSFKKNASLKAFNKMLDAVESGVKTNIPLSGVSTIYSNYKVAMNNVKTYHFIGKDATIDGTSYQIASTKEINRVSKLVRHALGLKAKKVTNNETKLYNYNVNYTSYDEYNNTEFTIPSSSSSYGSSSYGTSSSYGSSYGTTYGSTYGTSTGYGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKX5</accession>
    <name>H4GKX5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_11375</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000074">
        <property type="protein sequence ID" value="EHS84942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122707.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000074.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11375"/>
    <dbReference type="EnsemblBacteria" id="EHS84942">
        <property type="protein sequence ID" value="EHS84942"/>
        <property type="gene ID" value="PS3_11375"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1712"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008878">
        <property type="entry name" value="Transposase_IS66_Orf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36455">
        <property type="entry name" value="PTHR36455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05717">
        <property type="entry name" value="TnpB_IS66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS84942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="118" mass="13734" checksum="B6EEDA8873331AE8" modified="2012-04-18" version="1">MLINWQNPKHVYIVCGKTDLRKGIDGLAIVVAENFGLDLYDDSLFLFCGNRSDRFKGLYWDGEGFILLYKRFENGGLRWPRYRNDAHQLTNRQIKQLLEGVNPLPPKRVKTASHGDFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL09</accession>
    <name>H4GL09_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_14591</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122741.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS84775">
        <property type="protein sequence ID" value="EHS84775"/>
        <property type="gene ID" value="PS3_14591"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1757"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS84775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="79" mass="9257" checksum="970A866FB349CA14" modified="2012-04-18" version="1">MRIDIKAYLDHNGLTIYRVAKESGYGYTTLHKSFNKQQTNATSINLRDLDALAQTMHKQMWQILQELEDSYLIDDDKHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL27</accession>
    <name>H4GL27_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Purine deoxyribosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_9432</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122759.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9432"/>
    <dbReference type="EnsemblBacteria" id="EHS84657">
        <property type="protein sequence ID" value="EHS84657"/>
        <property type="gene ID" value="PS3_9432"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1779"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007710">
        <property type="entry name" value="Nucleoside_deoxyribTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05014">
        <property type="entry name" value="Nuc_deoxyrib_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS84657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="146" mass="16329" checksum="E59FC1163B1B6A53" modified="2012-04-18" version="1">MRIYLAGPFFSPEQIARIEIIETALRQNSTVEDFFSPRLGEDGGEFPMGSPEWANKVFHLGVSEIDRADALVVVADYVNDNMDSGTAFEVGYAYSHQKPIVVVHELPEPLNLMIGQALQCYLTDGHEVANLDFNNLPYQPYTGKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL37</accession>
    <name>H4GL37_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MazG nucleotide pyrophosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_12214</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122769.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12214"/>
    <dbReference type="EnsemblBacteria" id="EHS84667">
        <property type="protein sequence ID" value="EHS84667"/>
        <property type="gene ID" value="PS3_12214"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1792"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11541">
        <property type="entry name" value="NTP-PPase_u4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011379">
        <property type="entry name" value="MazG-related_GP37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004518">
        <property type="entry name" value="MazG_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03819">
        <property type="entry name" value="MazG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006639">
        <property type="entry name" value="UCP006639_pph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MazG" evidence="1">
        <location>
            <begin position="22"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="99" mass="11354" checksum="ABCC5638C90A9A10" modified="2012-04-18" version="1">MEFNEYQEAAKRTLYGNEQVLTNCALGLAGESGQLVDLVKNYTFKGHPLDRDDLIHEMGDVLWYLSQVAEWADIPFEEVAQKNIETLNERYPNGFNKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL53</accession>
    <name>H4GL53_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RsmD family RNA methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_3797</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122785.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3797"/>
    <dbReference type="EnsemblBacteria" id="EHS84683">
        <property type="protein sequence ID" value="EHS84683"/>
        <property type="gene ID" value="PS3_3797"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1808"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031167">
        <property type="term" value="P:rRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004398">
        <property type="entry name" value="RNA_MeTrfase_RsmD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004553">
        <property type="entry name" value="CHP00095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00095">
        <property type="entry name" value="TIGR00095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS84683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="187" mass="20739" checksum="50542B0423ECF3F1" modified="2012-04-18" version="1">MRVVSGKFRGRKLQAVPGMATRPTTDKVKESLFNMIGPYFSGGQSLDLYAGSGGLSIEGVSRGIDHAYLVDRQYQAIKTIKHNVALTKHENQFTIIKQDAQRILPVIAGQGVRLDLIYLDPPYAKQQIIKDIQSLVDLNLLNSGALVVCETNQEANLPTDLSQFELVRQQTYGITVVTIYRFIGEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL76</accession>
    <name>H4GL76_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 15 16">UDP-N-acetylmuramoylalanine--D-glutamate ligase</fullName>
            <ecNumber evidence="6 15 16">6.3.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">D-glutamic acid-adding enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">murD</name>
        <name evidence="20" type="ORF">PS3_6175</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 15 16">Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15 16">
            <text>ATP + D-glutamate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16429"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29986"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83898"/>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 15 16">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 15 16">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the MurCDEF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.9" evidence="6 15 16"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122808.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6175"/>
    <dbReference type="EnsemblBacteria" id="EHS84706">
        <property type="protein sequence ID" value="EHS84706"/>
        <property type="gene ID" value="PS3_6175"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1839"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008764">
        <property type="term" value="F:UDP-N-acetylmuramoylalanine-D-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00639">
        <property type="entry name" value="MurD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007698">
        <property type="entry name" value="AlaDH/PNT_NAD(H)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005762">
        <property type="entry name" value="MurD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01262">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01087">
        <property type="entry name" value="murD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13 15 16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9 15 16" id="KW-0132">Cell division</keyword>
    <keyword evidence="11 15 16" id="KW-0133">Cell shape</keyword>
    <keyword evidence="14 15 16" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15 20" id="KW-0436">Ligase</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 15 16" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AlaDh_PNT_C" evidence="17">
        <location>
            <begin position="8"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="19">
        <location>
            <begin position="117"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="18">
        <location>
            <begin position="312"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="119"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EHS84706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="458" mass="49959" checksum="55172CD5FA14E53B" modified="2012-04-18" version="1">MKAIKTYQGKRVLVIGFGISGVNAAKVLVKLGAKVTINDLKESDDQTTINELKALGIRIIMGANPLSLADEGFDLVVKNPGIPYTVPLIAKFVTQGTPIITELELGAQIFEGQIVAVTGSNGKTTTTTLIQQMLAKESQHQVVAAGNIGVSVSQQAQMLTSEDTMVMETSSFQLLGAPTFRPHIAVITNIFTSHLNYHGNRQNYINAKLNITRNQTKDDFLVINWDQEEWQELSRRTEAQVIPFSRENKSQAGAYQDHDQLFWQGEVVMSVADVSLVGPQNIENALAAIAVAKLSGVSNEAIREVLSSFSGVRHRLQYVMTAQGRKIYNDSKATDIEACEVALQGFNQPVILIAGGLDRGDDFSRLATVFKDHVKALITVGETKERLSQAGKQAGVPIVKESQDVITAAPMAWQLSEPGDVILLSPACASWDQFPSFEVRGDRFIETMETITGQKEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL90</accession>
    <name>H4GL90_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Global transcriptional regulator Spx</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">spx</name>
        <name evidence="7" type="ORF">PS3_3556</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Global transcriptional regulator that plays a key role in stress response and exerts either positive or negative regulation of genes. Acts by interacting with the C-terminal domain of the alpha subunit of the RNA polymerase (RNAP). This interaction can enhance binding of RNAP to the promoter region of target genes and stimulate their transcription, or block interaction of RNAP with activator.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with the C-terminal domain of the alpha subunit of the RNAP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ArsC family. Spx subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122822.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3556"/>
    <dbReference type="EnsemblBacteria" id="EHS84720">
        <property type="protein sequence ID" value="EHS84720"/>
        <property type="gene ID" value="PS3_3556"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1857"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01132">
        <property type="entry name" value="Spx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006660">
        <property type="entry name" value="Arsenate_reductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023731">
        <property type="entry name" value="Spx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006504">
        <property type="entry name" value="Tscrpt_reg_Spx/MgsR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30041">
        <property type="entry name" value="PTHR30041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03960">
        <property type="entry name" value="ArsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01617">
        <property type="entry name" value="arsC_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51353">
        <property type="entry name" value="ARSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS84720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="135" mass="15747" checksum="2087C6C1C0A05926" modified="2012-04-18" version="1">MVTLYVTPSSTSSRKARAWFEKHNIPFNERNIIANPLSIDEIKSILRMTENGTEDLISTRSKAFQALDVDLDEVPMNDLYQIIHDNPGMLRRPIMMDDKRLQIGYNEDEIRRFLPREIRQMELAKAQELAELLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL99</accession>
    <name>H4GL99_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA mismatch repair protein MutL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mutL</name>
        <name evidence="7" type="ORF">PS3_13036</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a 'molecular matchmaker', a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122831.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13036"/>
    <dbReference type="EnsemblBacteria" id="EHS84598">
        <property type="protein sequence ID" value="EHS84598"/>
        <property type="gene ID" value="PS3_13036"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1875"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032300">
        <property type="term" value="C:mismatch repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00149">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020667">
        <property type="entry name" value="DNA_mismatch_repair_MutL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="DNA_mismatch_repair_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042121">
        <property type="entry name" value="MutL_C_regsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="PTHR10073"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="SSF118116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3 4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_mis_repair" evidence="6">
        <location>
            <begin position="208"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="MutL_C" evidence="5">
        <location>
            <begin position="430"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS84598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="630" mass="70159" checksum="3E4D9C0ED0C37E4A" modified="2012-04-18" version="1">MGKIHELDRVLADQIAAGEVIERPASIVKELVENSIDAHSSRVDILVEESGLKEIRIIDNGDGIAADDLAIAFRRHATSKIQHQQDLFRVQTMGFRGEALPSIASVADVTMTTSTGQDAGNLIHLKGGELIKQQAASARPGTDITVKDLFFNTPARLKYLKSPRTELSRIVDLVNRLALAEPNIAFSLTHNGRELFRSAGNGNLQQVIAAIYGNSVGEKMLPLDAADPDFKISGYLSLPEMTRSSRQYMTVTINHRYIYNFQLTKAIITGYGSKLMVGRYPIVVLDVELDPLLVDVNVHPAKREVRLSKESQLANLISSSVEETISQMNLIPDVGQRAKELVEDHPSVTAVHEATTSPLEPITPNPPIIINARADLASPALHDFDQRYQTNPVKEPPSGNKASLEQTEELELAIEDHEKNSSDRFPNLTYMGQLHGTYLLAQATDGLYIIDQHAAQERINYEHYRQAIGEVNDDQQTFLEPLILNYSLADALKIQDHLATLKSVGLYLEAFGQNSFMLSSHPTWFVAGQEEATAREMIDWVIETGHLTVAEFREKTAIMMSCKRAIKANHYLDDQQAQALLARLPECENPFNCPHGRPVTVHFSPSDLEKLFKRIQDSHQPFADEFDDHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLA0</accession>
    <name>H4GLA0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA mismatch repair protein MutS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mutS</name>
        <name evidence="10" type="ORF">PS3_8090</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8090"/>
    <dbReference type="EnsemblBacteria" id="EHS84599">
        <property type="protein sequence ID" value="EHS84599"/>
        <property type="gene ID" value="PS3_8090"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1876"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00096">
        <property type="entry name" value="MutS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005748">
        <property type="entry name" value="DNA_mismatch_repair_MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="PTHR11361"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="SSF48334"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="SSF53150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="SSF55271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01070">
        <property type="entry name" value="mutS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 7 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_MISMATCH_REPAIR_2" evidence="9">
        <location>
            <begin position="677"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="603"/>
            <end position="610"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS84599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="862" mass="98029" checksum="AB38A764DE890DEE" modified="2012-04-18" version="1">MAKATPMMEQYQKIKDQYPDAFLFYRLGDFYELFNDDAIKGSQILELTLTSRSKNADNPIPMCGVPHRAVDSYIDILIDKGYKVAICEQMEDPKLTKGMVKREVTRLVTPGTQMDLGGEQSRQNNYLTALIERNGHFDLAYTDLSTGELKTTSVDRWSMALNELVNLQTREVVVNELTEEYRQQLLRQNILLSQQKQMADQAEVTYLTQDVVTSEQKQVVTLLVSYLLTTQKRSLDHMQRAVSYQLDAYMKIDHHSKANLELMTNMRSGKRQGTLAWLMDETKTAMGSRLLKQWLERPLIDEQAIIERQDKVQVFLDHYFERQNLQAELTKVYDLERLAGRVAYGSVNGRDLIQLKTSLQQVPKIRYILEEMDSPIFDQFVDQLDPLTDIADAIERAIKDEPPISVTDGDLIKDGYHRQLDDYRDAMQNGKQWIADLQAKERNLTGINNLKIGYNHVFGYYIEVTKANLDRLPEGRYERKQTLTNAERFSTPELKEREALILEAQEKSTALEYELFVALRDQVKAEIPRLQELAKNLSEIDVLQSFAVVSENYHFVRPTLNHDHNLEIIAGRHPVVEKFLGHQAYVPNDVLMDPETAILLITGPNMSGKSTYMRQLALIAVMTQVGCFVPAESARMPIFDQIFTRIGAADDLVSGESTFMVEMMEANRALSNATANSLILFDEIGRGTATYDGMALAQSIIEYVHAHIGAKTLFSTHYHELTSLDHELMHLKNVHVGATEENGELVFLHKVTDGPADKSYGIHVAKLAGMPGDLLHRANEILSRLEGQAPEEAIPLSEAESTSNVTKVKEATGQLALFDEPESKPVADQHEKLYQQIKSLNLMGMTPMEVMNQVYQWQQKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLB0</accession>
    <name>H4GLB0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Cell shape-determining protein MreC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 5">Cell shape protein MreC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_15374</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in formation and maintenance of cell shape.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the MreC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122842.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15374"/>
    <dbReference type="EnsemblBacteria" id="EHS84609">
        <property type="protein sequence ID" value="EHS84609"/>
        <property type="gene ID" value="PS3_15374"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1888"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042177">
        <property type="entry name" value="Cell/Rod_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042175">
        <property type="entry name" value="Cell/Rod_MreC_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007221">
        <property type="entry name" value="MreC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34138">
        <property type="entry name" value="PTHR34138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04085">
        <property type="entry name" value="MreC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038471">
        <property type="entry name" value="MreC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00219">
        <property type="entry name" value="mreC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="72"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR038471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS84609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="284" mass="30196" checksum="D0BC6FBC5632F5E1" modified="2012-04-18" version="1">MRDFFAKHRVISIVIGLFIAVGLMAGSIGIRNNRNTPTIVQQIGNTVVGWFGGIVNYPMNSANGTVSSLGQILNAYSENKTLKKKVSALEQTQTTNETLKKENKQLKAELELNNSLTDYTTITAAVTNRTPSAWENQLIINKGSSSGVKKNMAVLSGSGLIGRIVEVNPTNSKVELISSSSESTNQFSVAVDNEDGDTVNGLISGYDTKTSQLVMSEVSSSAAVKKGAKVTTNGLGGVTPKGLYVGKVVKTTKGSGGEIDRIYVEPATNLNDVNYVTIAELNED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHQ8</accession>
    <name>H4GHQ8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N6_Mtase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_19147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000003">
        <property type="protein sequence ID" value="EHS87553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19147"/>
    <dbReference type="EnsemblBacteria" id="EHS87553">
        <property type="protein sequence ID" value="EHS87553"/>
        <property type="gene ID" value="PS3_19147"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.20"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006306">
        <property type="term" value="P:DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003356">
        <property type="entry name" value="DNA_methylase_A-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016843">
        <property type="entry name" value="S-AdoMet-dep_Ade-MeTrfase_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02384">
        <property type="entry name" value="N6_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026567">
        <property type="entry name" value="Adenine_mtase_bact_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N6_Mtase" evidence="1">
        <location>
            <begin position="55"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="293" mass="32558" checksum="32DC348922FD73BF" modified="2012-04-18" version="1">MEDGIPNETDQAKLVHLYQRLAEFKFNPETMRQLIQLSMLKVTRKDQLPSNYQITPDTIGMLMAALASRILPEQETLSILDPVVGSANLLTTMMNQLQALNGRSTTGFGIDNDDLMLATASVNVALQKADVELFHQDAILDLAVPQVDLVVADLPVGYYPLDDNTKNYRTRAKEGHSFVHHLLIEQSMNYLKPGGFGLFLVPQALFQSDEAQGLVEWIQSVAHMQGLLNLPTAIFANQQAAKSILLLQRQGASAHQVPQVLLGTFPTLKDPKEFTAFMQKTDQWVATNLQSMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHT0</accession>
    <name>H4GHT0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Extracellular protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_5699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121614.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5699"/>
    <dbReference type="EnsemblBacteria" id="EHS87486">
        <property type="protein sequence ID" value="EHS87486"/>
        <property type="gene ID" value="PS3_5699"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.49"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018672">
        <property type="entry name" value="DUF2140"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09911">
        <property type="entry name" value="DUF2140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="195" mass="22103" checksum="BC5845EA9FA1B23C" modified="2012-04-18" version="1">MKKIKPSLWKWLFIGLLTINLAVIGTVIYQGTAPLTNVAETKAPKSSDSSLELVLSRNQVNTLSSNYLNQFLKDKTIKYQFIVGKKYASVIGVTKFMGVQVKFALNFVPEKTSQGNVILHAKSLSVGQLKLPMSYIMSYIKKNYTLPDWVSLNQKKRTILLDLNKYSQKKAINYHVNEINMDQGQFRIGLTIPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHT4</accession>
    <name>H4GHT4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Dihydrofolate reductase</fullName>
            <ecNumber evidence="3 4">1.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_3327</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15009"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the dihydrofolate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.3" evidence="3 4"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121618.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3327"/>
    <dbReference type="EnsemblBacteria" id="EHS87490">
        <property type="protein sequence ID" value="EHS87490"/>
        <property type="gene ID" value="PS3_3327"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.53"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00158"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004146">
        <property type="term" value="F:dihydrofolate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006545">
        <property type="term" value="P:glycine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00209">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012259">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001796">
        <property type="entry name" value="DHFR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069">
        <property type="entry name" value="PTHR48069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00186">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000194">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51330">
        <property type="entry name" value="DHFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHFR" evidence="5">
        <location>
            <begin position="3"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="164" mass="18550" checksum="7FF357AB17A52E38" modified="2012-04-18" version="1">MAEISFVWAQDEAGWIGKDNALPWHLPADLKHFKAVTMGKPIVMGYNTYLSIGRPLPHRENIVITHRDLKLPGVTVLHSIDELQQVLEDRLADQAVAIIGGAGLFQQTLSLVNVLERTVIEGDFQGDKKMPLINYDQWTLTKRESVISNDPKTPSCHFEHWEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHT9</accession>
    <name>H4GHT9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-binding protein HU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_15348</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121623.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15348"/>
    <dbReference type="EnsemblBacteria" id="EHS87495">
        <property type="protein sequence ID" value="EHS87495"/>
        <property type="gene ID" value="PS3_15348"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.58"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020816">
        <property type="entry name" value="Histone-like_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01727">
        <property type="entry name" value="DNABINDINGHU"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00045">
        <property type="entry name" value="HISTONE_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="91" mass="9553" checksum="699AF66D83176D1A" modified="2012-04-18" version="1">MANKAELVNNVAASTGLTKKDATAAVDAVFSSIEASLAKGEKVQLIGFGNFEVRERAARKGRNPQTGEEIQIPASKVPAFKPGKALKDAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHW6</accession>
    <name>H4GHW6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_3774</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121650.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3774"/>
    <dbReference type="EnsemblBacteria" id="EHS87522">
        <property type="protein sequence ID" value="EHS87522"/>
        <property type="gene ID" value="PS3_3774"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.92"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="5"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="201" mass="21864" checksum="43CCAB62DB5E9A2E" modified="2012-04-18" version="1">MKHYIGIAGTHAKGSTNTKLIHFIKNHLADRAEVEIIDASNWPVFQKTPDGKLPEGVQAVADKIAAADGVIISTPEYDHTIPAALMNTFAWLSYGIHPFDNKPVMITGASFGALGTSRAQGHLRRMLDAPEIHAQTMASSDFMVSRSLNAFDEEGNLKDPALVKRFDELFAEFEVFVDLMAKLAQVNQVAEADAAKVGEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHY9</accession>
    <name>H4GHY9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Probable potassium transport system protein kup</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">kup</name>
        <name evidence="12" type="ORF">PS3_11019</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Transport of potassium into the cell.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the HAK/KUP transporter (TC 2.A.72) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000006">
        <property type="protein sequence ID" value="EHS87468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121673.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000006.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11019"/>
    <dbReference type="EnsemblBacteria" id="EHS87468">
        <property type="protein sequence ID" value="EHS87468"/>
        <property type="gene ID" value="PS3_11019"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.118"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01522">
        <property type="entry name" value="Kup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003855">
        <property type="entry name" value="K+_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023051">
        <property type="entry name" value="K+_uptake_low_affin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30540">
        <property type="entry name" value="PTHR30540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02705">
        <property type="entry name" value="K_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 11" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="248"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="293"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="401"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="425"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="667" mass="74192" checksum="469E9AB5FC8D5CD4" modified="2012-04-18" version="1">MKTKKTSLLAGGLVALGIVYGDIGTSPLYVMNALINDAGGAQNLTRDYIFGSLSLVFWTLMLMTTIKYVLVALRADNHGEGGIFALYALLRHKVKASWLIVPALIGGAAILADGTLTPAVTVTSAIEGLKGIKIGNQVWVHNQSEVVIVTLLILLILFIIQRFGTSILGKAFGPIMLIWFGFLAVFGIANLVDEPGVLLALSPHYGIELLFSPANKAGIFILGSVFLATTGAEALYSDIGHVGRPSIYLTWPFVCLSLVLNYFGQGAYLLHRIGSFSPTGTGYNPFYQMLPSWVYLFGVVIAALAAIIASQALITGSFTLIEEAIGLKFLPRLKVEHPSLARGQIYISAVNWILCLVTSCVLLYFRTSAHMEAAYGLAITMTMLMTTLLLFHYLQLKTKAGLAWLFAIVYVTIEGIFFISSMTKFVHGGYVTILITLLILLIMVGWYFGNKLRDSFASENEYLNLDNYKTMLAQLSNDDALPLTVSNLVMMTRVNRNHHVKREILYSILDKDLKRAKVYWFITVNTTDEPYTSEYTVDLMGTRNMVNVQLYLGFKMPTKVNLYLRQIVQDLMDQGQIDPQPQAYTTIKGRQVGDFKFVFIKELLSNDNQITGWKRGLINIRIWLQNHTLSPVNFYGLEFSDVITEPVPIVLHRQSPVKLKQRSVPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI03</accession>
    <name>H4GI03_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA(Ile)-lysidine synthase</fullName>
            <ecNumber evidence="7">6.3.4.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA(Ile)-2-lysyl-cytidine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">tRNA(Ile)-lysidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tilS</name>
        <name evidence="9" type="ORF">PS3_10781</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP + diphosphate + H(+) + lysidine(34) in tRNA(Ile2)</text>
            <dbReference type="Rhea" id="RHEA:43744"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10670"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83665"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the tRNA(Ile)-lysidine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.19" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000007">
        <property type="protein sequence ID" value="EHS87454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121687.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000007.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10781"/>
    <dbReference type="EnsemblBacteria" id="EHS87454">
        <property type="protein sequence ID" value="EHS87454"/>
        <property type="gene ID" value="PS3_10781"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.134"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01992">
        <property type="entry name" value="PP-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01161">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012796">
        <property type="entry name" value="Lysidine-tRNA-synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012094">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012795">
        <property type="entry name" value="tRNA_Ile_lys_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015262">
        <property type="entry name" value="tRNA_Ile_lys_synt_subst-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43033">
        <property type="entry name" value="PTHR43033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09179">
        <property type="entry name" value="TilS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11734">
        <property type="entry name" value="TilS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00977">
        <property type="entry name" value="TilS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02433">
        <property type="entry name" value="lysidine_TilS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02432">
        <property type="entry name" value="lysidine_TilS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TilS_C" evidence="8">
        <location>
            <begin position="369"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="450" mass="52452" checksum="662FEE68766D7AA0" modified="2012-04-18" version="1">MRGLQASIQKVLKKYWDGQSKLVVAVSTGVDSMLLLHELEQLHYPIVVAHVNHHLRTQSDQEAQFLVQYCRDHHIAIQIADWHVADHPDKGVEEAARQFRYRFFYQVMDKEQSRILLTAHHQNDLAETVLMKLVRGGWLKSIVGLQTARTFNTGWLLRPWLQISKEEILRQAQVIGLTWYEDQSNQSMVIQRNRFRHQILPALIKEDPQALAHLGDFHDQLTKLLTFAEAELSKYDEVLLDGQRLKVTEFNSLPSATQDLYLKHYFNQAHLQRLTKDQLNQALHLLSNQNKPQGQLDLSADWRLVKTYQYAWLQKGETAEVIKSPADVAMIELEELYSTDDGKLFWFSKHKSNDHLIASFNLTMEQLPLQIRKWQPGDRLRLKNGGHQKIHRILIDQKVPQSKRTHQQVVVDQFDQVLWLVGRKTAWVTSQGATQEIYLYYQGDNHDNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI08</accession>
    <name>H4GI08_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alanine racemase</fullName>
            <ecNumber evidence="3">5.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_10780</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-alanine = D-alanine</text>
            <dbReference type="Rhea" id="RHEA:20249"/>
            <dbReference type="ChEBI" id="CHEBI:57416"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="5.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alanine racemase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000007">
        <property type="protein sequence ID" value="EHS87459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121692.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000007.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10780"/>
    <dbReference type="EnsemblBacteria" id="EHS87459">
        <property type="protein sequence ID" value="EHS87459"/>
        <property type="gene ID" value="PS3_10780"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.142"/>
    <dbReference type="UniPathway" id="UPA00042">
        <property type="reaction ID" value="UER00497"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008784">
        <property type="term" value="F:alanine racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030632">
        <property type="term" value="P:D-alanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01201">
        <property type="entry name" value="Ala_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000821">
        <property type="entry name" value="Ala_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011079">
        <property type="entry name" value="Ala_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020622">
        <property type="entry name" value="Ala_racemase_pyridoxalP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00842">
        <property type="entry name" value="Ala_racemase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00992">
        <property type="entry name" value="ALARACEMASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01005">
        <property type="entry name" value="Ala_racemase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00492">
        <property type="entry name" value="alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00395">
        <property type="entry name" value="ALANINE_RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ala_racemase_C" evidence="6">
        <location>
            <begin position="247"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; specific for D-alanine" evidence="3">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; specific for L-alanine" evidence="3">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3 4">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600821-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR600821-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="373" mass="40343" checksum="877D908F6625CB86" modified="2012-04-18" version="1">MVTANLRDAQVEVNLAAIRHNVAAQRQAMPAGNRIFGVVKANAYGHGAVAVAQDLSSAGIDGFCVAALDEGLELRQAGLTELILVLGITPVQYAEVAAENDISLTVGDVDWLKAYQQLAHTVSIPKPLKVHLGFDTGMGRIGFTEPALLKEALELLKDPVFEFEGCFTHFATADEANDAYFKLQIAHWHELLAVLPTLPPYVHMANSATGLWHPDQITGNTVRMGISMYGLNPSGTVLPATLDLQPALAVTARLTFVKQLKAGHSVSYGATYTAKQDEWIGTIPVGYADGYARQLSGYQALINGQRCDLVGRVCMDQMMVRLPQELPVGTKVTLLGRDGNDEITATELAEQTGTINYEILTSLSPRLKRIYKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI38</accession>
    <name>H4GI38_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamine synthetase</fullName>
            <ecNumber evidence="4">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_9003</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the glutamine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121722.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9003"/>
    <dbReference type="EnsemblBacteria" id="EHS87377">
        <property type="protein sequence ID" value="EHS87377"/>
        <property type="gene ID" value="PS3_9003"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.180"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamate-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_b-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004809">
        <property type="entry name" value="Gln_synth_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="SSF54368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00653">
        <property type="entry name" value="GlnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gln-synt_C" evidence="5">
        <location>
            <begin position="107"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-AMP-tyrosine" evidence="2">
        <location>
            <position position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="446" mass="50261" checksum="3AE839ECB3BBAFA6" modified="2012-04-18" version="1">MAKHVYTNDEVRELIKKEDVRFLRLMFTDLFGTMKSVDLPISQLDKLLANKVMFDGSSIDGFVRIEESDMYLYPDMGTWLISPWGAEHGKVAQVICSVHLTNGEPFMGDPRNNLKRVLSEMKDAGFTAFNIGPEPEFFLFETDENGEPTLKVNDEENYFDLEAADKGEDCRRDIVLALEKLGFDVEAAHHEVAPGQHEVDFKYSDALEAADNIQIFKLVVKTIAKKYGFHATFMPKPLSGINGSGMHMNMSVFDQDGNTFYDENGKLQLSETAYKFLAGVMKHARAVTAICDPTVNSYKRLVPGYEAPVYVAWSAHNRSPMVRVPSDRGMGTRLELRSVDPSANPYLAIAAILAAGLDGLKNDLKPADSVDENIYTMTEEERVSREILTLPDTLHNALKSLATDEVVKAALGDHLFYNFMEAKTMEYDAYRQSVSRWEQDRYMSTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI42</accession>
    <name>H4GI42_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Rhomboid family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_9741</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121726.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9741"/>
    <dbReference type="EnsemblBacteria" id="EHS87381">
        <property type="protein sequence ID" value="EHS87381"/>
        <property type="gene ID" value="PS3_9741"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.186"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002610">
        <property type="entry name" value="Peptidase_S54_rhomboid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22936">
        <property type="entry name" value="PTHR22936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="SSF144091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Rhomboid" evidence="6">
        <location>
            <begin position="50"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="221" mass="24365" checksum="74881D49906C5A95" modified="2012-04-18" version="1">MIEAFKRTPVTITIIVIRVLVFIAMELNGGSTNTVTLLNFGAEYTPLLVAGQWWRLITSAFVHIGIMHLLLNSIVLYYMGNYIEQLFGHWKLILIYIISVISGNLLSAALSPTSIAAGSSTGIFGLFGAFIFLGAEHRQQSFLRILTRQYIILMIINLVFDLMSPSIDIWGHLGGLLAGFLAGALFDPEQFGKIRLVKRLLSAAILILILASLTRMVLLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI43</accession>
    <name>H4GI43_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Penicillin binding protein 2B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_11367</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transpeptidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11367"/>
    <dbReference type="EnsemblBacteria" id="EHS87382">
        <property type="protein sequence ID" value="EHS87382"/>
        <property type="gene ID" value="PS3_11367"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.188"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005311">
        <property type="entry name" value="PBP_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036138">
        <property type="entry name" value="PBP_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03717">
        <property type="entry name" value="PBP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56519">
        <property type="entry name" value="SSF56519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_dimer" evidence="8">
        <location>
            <begin position="75"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="7">
        <location>
            <begin position="350"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="606"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="690" mass="74829" checksum="877EB1330FB06D97" modified="2012-04-18" version="1">MLHKNSNQSTRSRERRRRRRRVKSSTLPIRLHVLLWIAGILMVMLGARLFYMQILQGSYYKSAVRNSNTYTRTTNVQRGLIFDSTGSALVTNQAHKAVTYTKSSGTSSSDVYKLANRLSKYLTVDTDNLSTADQEAYYLANTKNYQAVLKKIKTSDADSSTEQYEKALRYLEKHPSVYKLTASQKNSAQLYTTMNSSYTMSTTYLKKSGVTSKEIAEIGEHLSQLPGIKLSTAWTRKYADNDITSLTGSVSTNGLPSDQLKTLLAQGYSRNDSVGTSLLEKQYQSALAGIKGTTKITTNGNKVTKEKTTYAGQRGSSLVLTINYNFQKKVQQILSDNYSSAGNTYSTGVYAVVMNPNTGAVYAMAGIDRDPSTGKETVDETGAITQAMTMGSVVKGATLIAGFLEGVITPSNNTLTDMPIKLAGTSAKTSWFNKTGSANQSLTAAQALQVSSNSYMMQIAMKIGGFTYRSGAHLALKSNIFSKLRYYYNMVGLGTKTGIDLPGEVSGYEGSSTSSDQGKALDLSYGNYDEYTVMQLAQYMSTIANGGKKMKPYLVKQIRQTTSTGSLGKVEYTAHPTVQSTLPVTKSELNIIKRGLYLVTHGTDSNTTAKSLGSETPSISGKTGTAETYYNGNSTTTLSFAGYAPSDNPQVVVALAINGATDDDSGANITMAKEIFSAYWSMVKNNSSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI44</accession>
    <name>H4GI44_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_15381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121728.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15381"/>
    <dbReference type="EnsemblBacteria" id="EHS87383">
        <property type="protein sequence ID" value="EHS87383"/>
        <property type="gene ID" value="PS3_15381"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.189"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018580">
        <property type="entry name" value="Uncharacterised_YfhO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38454">
        <property type="entry name" value="PTHR38454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09586">
        <property type="entry name" value="YfhO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="358"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="382"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="406"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="436"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="834"/>
            <end position="858"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="870" mass="98236" checksum="4870275DC7D9C803" modified="2012-04-18" version="1">MLTKLNQRRNILALSFLVPVLIMGCYFTLRGMAPFGKSSILTVDLGQQYVDFYAYLRSSILHHPASLLYSFSKGLGGEMWGTNAYYLMSPLNLLLLFFPGASLSSGILFLLLIKYGLAGLSMAYLLDRSNLQSGYRLLMFSTPYALMGWMIANQLNMIWLDVLWLLPLVIVGLMQLLDGGRPWFYLSWLAITMIVNYYMAWMVCVFTILFTWWHLSLSRYPWRQKIRRFLRYVIYSCWSGLLSAVILLPTIYALTQSKGTYNNTDYTWKLEYNPLKLFGKLVPGSFNFSQMPSGQANIYVGMLMMTGFLLYFFNRRDRKWSRIIAALISIFLVASFVVQPLDLFWHLGQFPVWYPSRFSFLFSFWVIWLAARTLTPNFQLKWPQLLFILAISSLITVVLLTKVNSILSYINTTQIWIGYSFELLALIALSIRRHDYFYHGFLILLACVDVSVSAILSLNQISYVSQSDYGNYTTQLQQSIKKIKQADQSFYRIAKSFSRTKDDPFQADFYSGDHFGSTLEPSISKFMTLIGQPAGDGVISYANGTKITDSLLGFKYNLSTTATNDSALGVNTIRPDWTGKTIAKNGKITIKQNSTALPIAFGASQKILNLKSSSSDPTQYQSMIYQALAGKSTNQSLFKVQNFSNVTYTNTNETTQITGAMIVKNDPLLVGKVELTFTPQTNDPYYLTLGESAVANATIEVNGRPVTNVSSFRNTIIMNVANHQKGQSVKITFTLKKASLWLQNVSLYQLNQAGFQADYQKLSQSPFKISKSGSNYLTGTIKLARNQKVMMTTIPAAQGWHAIVDGHPAKLTKVINTFWALKLSPGKTYRITLYFIPPFLFTGLIMTIISLTGAIIMAKRKAKNVIKKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI76</accession>
    <name>H4GI76_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">DNA polymerase I</fullName>
            <ecNumber evidence="3 13">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">polA</name>
        <name evidence="17" type="ORF">PS3_10198</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2 13">Single-chain monomer with multiple functions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the DNA polymerase type-A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="3 13"/>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121760.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10198"/>
    <dbReference type="EnsemblBacteria" id="EHS87415">
        <property type="protein sequence ID" value="EHS87415"/>
        <property type="gene ID" value="PS3_10198"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.230"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09898">
        <property type="entry name" value="H3TH_53EXO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020046">
        <property type="entry name" value="5-3_exonucl_a-hlix_arch_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002421">
        <property type="entry name" value="5-3_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019760">
        <property type="entry name" value="DNA-dir_DNA_pol_A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020045">
        <property type="entry name" value="DNA_polI_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018320">
        <property type="entry name" value="DNA_polymerase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002298">
        <property type="entry name" value="DNA_polymerase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133">
        <property type="entry name" value="PTHR10133"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01367">
        <property type="entry name" value="5_3_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02739">
        <property type="entry name" value="5_3_exonuc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00868">
        <property type="entry name" value="DNAPOLI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00475">
        <property type="entry name" value="53EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="SSF47807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00593">
        <property type="entry name" value="pola"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00447">
        <property type="entry name" value="DNA_POLYMERASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 13" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="53EXOc" evidence="15">
        <location>
            <begin position="2"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="3'-5' exonuclease" evidence="14">
        <location>
            <begin position="298"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="POLAc" evidence="16">
        <location>
            <begin position="639"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EHS87415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="887" mass="100018" checksum="7F8489324B0193CA" modified="2012-04-18" version="1">MAKQLLLIDGNSIMFRAFFAMHNQLERFTNQDGLHTAAIYGFKLMLDHVLAEYQPDAALVAFDAGKVTFRTQLFDDYKGSRDKTPSELTEQIPYVRRLVQDSGIQSYELPNYEADDIIGTLAQEADQAGYETTIITGDRDLTQLVTPQTTVAVTKKGVTQTEDYTPEYMQEKLGVTPVQYIDVKALMGDSSDNYPGVTKIGEKTAVSLIQEYGSLDGLYEHVQDMKPSKRKENLINEEKIARECQTLATIKRDAPVEVQLTDLTYQGPDQEALAELYRELNFQSFLSQMGVEAPVPTVKPVQYVELTADNLSLLDQFKTSLVFHLEMLEANYHLAQPVGFVLGSPDCWLVSRDINLLQKPAIKQLLANDEIKKDVFNAKAQLVALHRLGVSLHHVAFDLLLASYLLDTNENSDDLGLLAQVHDYHDVQPDEVVYGKGAKRAVPEDNEIFYTHLANKARAIYELRQPLFDQLKANEQLSLYTDIERPLSAVLAQMEIDGVMVKTSTLTQMQSQLTERLSELEQAIYQAAGEEFNINSTKQLGVILFEKLKLPVIKKTKTGYSTAVSVLEKLRDQAPVVDLILQYRQIAKIQSTYVTGLLKVIHPDDHKIHTRYLQTLTQTGRLSSVDPNLQNIPVRVEEGRRIRQAFVPSEPGWEIFSSDYSQVELRVLAHVSGDQNMQADFINGEDIHAATARRIFHLLPDEEVTPNMRRQAKAVNFGIVYGISDYGLAQNINVSPKQAKTFIESYFAEFPGVHDYTKSIVETARQQGYVETITHRRRYLPDIHAKNFNLRSFAERTAMNTPIQGSAADIIKIAMIRMGPALAAHHLQARMLLQIHDELVFEAPKEELATLRQVVTEVMDSAVKLSVPLKVESKVGPTWYDLKKIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI79</accession>
    <name>H4GI79_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_7782</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121763.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7782"/>
    <dbReference type="EnsemblBacteria" id="EHS87418">
        <property type="protein sequence ID" value="EHS87418"/>
        <property type="gene ID" value="PS3_7782"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.234"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="108" mass="12431" checksum="8E82430F33759C9E" modified="2012-04-18" version="1">MKDLPKTNLTDLEAQLQSGRYILFFTADWCPDCRFIKPAMPEIEAEYADFTFIKVDRDENIDLAAEMGIMGIPSFVAYQDGQEIGRFVNKDRKTKQEVETFINGLNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI93</accession>
    <name>H4GI93_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_9847</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121777.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9847"/>
    <dbReference type="EnsemblBacteria" id="EHS87314">
        <property type="protein sequence ID" value="EHS87314"/>
        <property type="gene ID" value="PS3_9847"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.257"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043429">
        <property type="entry name" value="ArtM/GltK/GlnP/TcyL/YhdX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614">
        <property type="entry name" value="PTHR30614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="20"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="217" mass="24422" checksum="7B85D1908027C299" modified="2012-04-18" version="1">MQTFIDAYSWINIRFLLEGLWVTIEISVVSILISLIVGILLGTIRYMRIKYVSAIVGFVIDIIRNLPLLLIIFFVYFGLPNIGFRPPVIPSAIMSMSLFESMMIAEIVRSGLQGIDIGQTEGAMANGMTHWQALRIVVLPQGLKHMLPALVSQFISLVKDTSLATIIVLPELMYHAEIVYGQNSTYIIPMFVAIAVMYFIVCYLLSLLSRYLDHKMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIA6</accession>
    <name>H4GIA6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipoate--protein ligase</fullName>
            <ecNumber evidence="4">6.3.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_11338</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-lipoate + ATP + L-lysyl-[lipoyl-carrier protein] = (R)-N(6)-lipoyl-L-lysyl-[lipoyl-carrier protein] + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10500"/>
            <dbReference type="Rhea" id="RHEA-COMP:10502"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 2/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.20" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121790.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11338"/>
    <dbReference type="EnsemblBacteria" id="EHS87327">
        <property type="protein sequence ID" value="EHS87327"/>
        <property type="gene ID" value="PS3_11338"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.271"/>
    <dbReference type="UniPathway" id="UPA00537">
        <property type="reaction ID" value="UER00594"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016979">
        <property type="term" value="F:lipoate-protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019491">
        <property type="entry name" value="Lipoate_protein_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004562">
        <property type="entry name" value="LipoylTrfase_LipoateP_Ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12561">
        <property type="entry name" value="PTHR12561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10437">
        <property type="entry name" value="Lip_prot_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00545">
        <property type="entry name" value="lipoyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="8">
        <location>
            <begin position="26"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="337" mass="38171" checksum="815DB5C9E7EAAE62" modified="2012-04-18" version="1">MKYLVTKSHDIRYNLALETYLMEHADLSEPILYFYINSPCIILGRNQNAYEEVNLDYVKAHQIVVTRRTSGGGAVFDDLGNVSFSFITQDDGHSSGNFRKFTDPVIQALHEMGATKAELSGRNDLQINGQKFSGNAMRVEHGRMFSHGTLMYDVDLDVIGQALNVPADKIASKGIKSVHSRVTNLKPHFAPEFQNLTIEEFRDTLAKKILGLTDLKDARPYELDQATLQAVDQLNQAVYSNWDWVYGKSPVSKIQHRQHFDQGTVDFRLEINDGRITDLQVYGDFLGQGDVNEFAKQLRGVRYQENELADALSRIDTEYYFGKIAPADLLALLLRQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIE2</accession>
    <name>H4GIE2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_17351</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17351"/>
    <dbReference type="EnsemblBacteria" id="EHS87363">
        <property type="protein sequence ID" value="EHS87363"/>
        <property type="gene ID" value="PS3_17351"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.336"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="225" mass="25443" checksum="F6EC552B99B78C95" modified="2012-04-18" version="1">MSEKVANTFDGRLIFLMLSILLNVFFNALTVSTNMGSAVWTASAVNLAHLFNLSLGNTLFLEGVIVAIVNLILLGQFDYFRLFRNLIFIVPYSYLLAWFKQLFFAMGIPLLPIGWRFVLDLVGLTGVAVAVSLYQRANVVLHPNDDLPFILRFRFLHGSPCWSQWDSNIPPIAIIVVTAILTHQLFAVNIGTILNFVMQGYIIGWADQHIFPQLHHHLDFKSTLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIF6</accession>
    <name>H4GIF6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extracellular surface protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_10555</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000015">
        <property type="protein sequence ID" value="EHS87305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121840.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000015.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10555"/>
    <dbReference type="EnsemblBacteria" id="EHS87305">
        <property type="protein sequence ID" value="EHS87305"/>
        <property type="gene ID" value="PS3_10555"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.353"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="SSF54106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003591848" evidence="2">
        <location>
            <begin position="33"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="3">
        <location>
            <begin position="34"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="197" mass="21057" checksum="9FCBF6AA90868141" modified="2012-04-18" version="1">MFKFKKKFLIQLTTTLGALALGVTAAVTVANADTTYTVKSGDTLNSISQQYVGDKSLVSSIVKANNISDQNVIYVGEQLTIPTDSSDNASTNTDSSTTSDNSSNTDSNSSTTTDSSSYTSSVSGSEAEAKEWIAQKESSGSYTASNGNYYGRYQLSLSYLNGDLSASNQEKVADQYVASRYGSWTAAKAFWEANGWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIH3</accession>
    <name>H4GIH3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP-grasp protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_4940</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121857.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000017.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4940"/>
    <dbReference type="EnsemblBacteria" id="EHS87294">
        <property type="protein sequence ID" value="EHS87294"/>
        <property type="gene ID" value="PS3_4940"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.374"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="125"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="408" mass="47521" checksum="96B015A52266ADAE" modified="2012-04-18" version="1">MKTQVDFQPVLLGTDFNCYGMARSFYHEYGIKSIAFGVMHMAPTRYTKLVDVRVVEGFNSNPVFMETLTAFAKTMDPNKKYLLLSCGDGYTELISEHLTELKQWFVCPYVDNELFKRLGSKETFYAAAEEYGLPYPKTKLISREMVERGEKIELPFDFPVALKPSDSIAWLDVDFEGRKKAFILDSRQQFDELLPKIYQAGYTGKMICQDFIPGDDSNMRVVNAYVDSDHHVRMISLGHPLLEDPSPAAVGNYMVILPEKNDQIYQTIQKFLEEIKYVGFADFDLKYDRRDDTYKVFEINLRQGRSSFYCTLNGYNLAKYVVEDVIYHQPFTETEYADGDQLWLGVPKKIFYQYAKESEYKDRAVKLLKAGKYGTTAFDKDDTLKQRMLMHYAFYLMPGNYKKYFVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIH5</accession>
    <name>H4GIH5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">UDP-N-acetylmuramoylalanyl-D-glutamate-2, 6-diaminopimelate ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_17148</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MurCDEF family. MurE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17148"/>
    <dbReference type="EnsemblBacteria" id="EHS87296">
        <property type="protein sequence ID" value="EHS87296"/>
        <property type="gene ID" value="PS3_17148"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.376"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035911">
        <property type="entry name" value="MurE/MurF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005761">
        <property type="entry name" value="UDP-N-AcMur-Glu-dNH2Pim_ligase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63418">
        <property type="entry name" value="SSF63418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01085">
        <property type="entry name" value="murE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="3" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="5">
        <location>
            <begin position="127"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="4">
        <location>
            <begin position="350"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="507" mass="57041" checksum="802FE02F765BED7F" modified="2012-04-18" version="1">MSLQGAIDLLKAHQLFEEVIINQQRHYDLSDSLKDQPINQLTYDSRQVEAQALFICKGLHFNTQYLIDAIHKGAVAILSGQEQVEIVKAQQLRALQIVVNDVQKAMAVLARGFYGNPDEQLTMIGYTGTKGKTTSVYFARHILQAQYGSNVAQLSSIDNCLNGTDFVESHLTTPESLDLFRMLREAVDHGMKYLVMEVSSQAYKKSRVYGLQFDYGVFLNISPDHISPVEHPNFDDYLYCKSQIIEHSKQIIISREIAAYDLLIEKARERNVPVISFGVRPGCDYQYQAEEHGYFKVKTNRDRLPRLDDQYRVMVPGNFNYANALSAMIVAAQLKIKPATIKQGLLETVVPGRMEMMTSPNGIIACVDYAHNYLSLTASFAFLKHEYPQSRLIVVVGSVGDKAESRRSGIAKALMEYADIAILTSDDTYGEDQQKVIQEIHSYLSGPKPVEVLEILDREAAVRQAFALAQPQDVVFLAAKGREQYLRVASGNQPYFGDYQLAQELTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIK2</accession>
    <name>H4GIK2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Peptide chain release factor 2</fullName>
            <shortName evidence="6">RF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">prfB</name>
        <name evidence="9" type="ORF">PS3_20860</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">Methylated by PrmC. Methylation increases the termination efficiency of RF2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20860"/>
    <dbReference type="EnsemblBacteria" id="EHS87197">
        <property type="protein sequence ID" value="EHS87197"/>
        <property type="gene ID" value="PS3_20860"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.412"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00094">
        <property type="entry name" value="Rel_fac_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004374">
        <property type="entry name" value="PrfB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00020">
        <property type="entry name" value="prfB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0488">Methylation</keyword>
    <keyword evidence="5 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RF_PROK_I" evidence="8">
        <location>
            <begin position="205"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="241"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="6">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="332" mass="37598" checksum="5A2837C7CCBDE4A3" modified="2012-04-18" version="1">MTVPGFWDDNQQAQKLIDETNQLKGRRDTFLSLQTAVNDLIDLRDLLSLEADADLEKELENNLRRTTSEVEKYQLNQLLDGPYDANNAILEIHPGAGGTEAQDWGEMLLRMYTRWAEQHQYQVEIANYEAGEEAGIKSVTLIIKGHNAYGFLHPEKGVHRLVRISPFDSANRRHTSFASIDVMPEMDDSIEIDINSADLRVDTFRSSGAGGQHINKTDSAIRITHLPTGIVTSSQAERSQLQNRETAMRMLKSKLYELEQEKQAAERAKIAGEQLENGWGSQIRSYVLHPYSLVKDTRTGYETHDTQGVLDGDLDPFINAFLKWQLSQKNPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIK5</accession>
    <name>H4GIK5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_9735</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121889.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9735"/>
    <dbReference type="EnsemblBacteria" id="EHS87200">
        <property type="protein sequence ID" value="EHS87200"/>
        <property type="gene ID" value="PS3_9735"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.415"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032524">
        <property type="entry name" value="ABC_tran_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16326">
        <property type="entry name" value="ABC_tran_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="4"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="329"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="553"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="361"/>
            <end position="368"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="642" mass="73376" checksum="7427DAF8CC7EEDA9" modified="2012-04-18" version="1">MLLLQTNNVERRFGADTLFHKINLQINDHARIALVGRNGAGKTTLLRIIAGITTPDEGEVTTIRDLTIGYLAQDQGLDSQNSIWAEMDSVFAPLHQMEAAIHDLESQLATIDPTSDDFANITGQYDQLQTKFQSQGGYEINSKIRGVLTGFGFTEQMYDQPVTDLSGGQKTKLALAKILLERPQLLILDEPTNHLDMNVLTWLESYLKTYRGALLIVSHDRYFLDRVVNEVYDLDQKTLRHYTGNYSQFVDHKADWLQSEMKHYDQQQKEIAKLEDFVNRNLVRASTTKRAQSRRKQLEKMERMERPTTDDRSIHFQFHSEHESGNEVLEVDQLSIGYHDQKIAGPLSFTERKGQRLGIIGPNGIGKSTLLKTILNLLPPISGTVKLGANLEIGYYDQEQQQLHPEKTVLDEVWDEHPEVDETEIRTLLGSFLFIGDDVYKQVKDLSGGQKARLELTKLSFQPMNFLVLDEPTNHLDIDSREVLETAINEFTGTVLFVSHDRYFINQVATQLVAIHPDHATSYEGDYDDYLAALDQSHDQVSEETTNEVAKPKQESYQATKEIQRERRKLQRKVDEYEETMEKLDQQIAKIQETMNQPEVASDIAKLTDLQKELDHYQAQADEAELAWTETAEALESFDQDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIK9</accession>
    <name>H4GIK9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta</fullName>
            <shortName evidence="1">ACCase subunit beta</shortName>
            <shortName evidence="1">Acetyl-CoA carboxylase carboxyltransferase subunit beta</shortName>
            <ecNumber evidence="1">2.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">accD</name>
        <name evidence="3" type="ORF">PS3_6720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the acetyl coenzyme A carboxylase (ACC) complex. Biotin carboxylase (BC) catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the transcarboxylase to acetyl-CoA to form malonyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + N(6)-carboxybiotinyl-L-lysyl-[protein] = malonyl-CoA + N(6)-biotinyl-L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:54728"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="EC" id="2.1.3.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Acetyl-CoA carboxylase is a heterohexamer composed of biotin carboxyl carrier protein (AccB), biotin carboxylase (AccC) and two subunits each of ACCase subunit alpha (AccA) and ACCase subunit beta (AccD).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AccD/PCCB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.15" evidence="1"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121893.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6720"/>
    <dbReference type="EnsemblBacteria" id="EHS87204">
        <property type="protein sequence ID" value="EHS87204"/>
        <property type="gene ID" value="PS3_6720"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.420"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009317">
        <property type="term" value="C:acetyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016743">
        <property type="term" value="F:carboxyl- or carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01395">
        <property type="entry name" value="AcetylCoA_CT_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000438">
        <property type="entry name" value="Acetyl_CoA_COase_Trfase_b_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01070">
        <property type="entry name" value="ACCCTRFRASEB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00515">
        <property type="entry name" value="accD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="2">
        <location>
            <begin position="29"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="279" mass="30869" checksum="3B6AAC3E68AAE0BB" modified="2012-04-18" version="1">MKLYRKSNTLSEHHIKADPAANQKVPDGLWQKCPKCQTALFMKQLDQYVTCPKCDYGFRISAQDRLKWLVDDFQPMDTDLQTADPLDFPGYLDKIQRAQQQTGLNDSVATGRAVINQTSFNLGIMDPTFIMGSLGSVTGEKITRLFEDATQDRLPVVLFTASGGARMQEGITSLMQMAKVSSAIARHSEAGLLYIVVLTDPTTGGVTASFAMLGDIILAEPHAMVGFAGRRVIEQTMHEKIPANLQEAENVLDHGFIDQIVKRQDEKATIEWLLQNGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIQ2</accession>
    <name>H4GIQ2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribose-phosphate pyrophosphokinase</fullName>
            <shortName evidence="6">RPPK</shortName>
            <ecNumber evidence="6">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">5-phospho-D-ribosyl alpha-1-diphosphate</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosyl pyrophosphate synthase</fullName>
            <shortName evidence="6">P-Rib-PP synthase</shortName>
            <shortName evidence="6">PRPP synthase</shortName>
            <shortName evidence="6">PRPPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">prs</name>
        <name evidence="8" type="ORF">PS3_18762</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ribose-phosphate pyrophosphokinase family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121936.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18762"/>
    <dbReference type="EnsemblBacteria" id="EHS87247">
        <property type="protein sequence ID" value="EHS87247"/>
        <property type="gene ID" value="PS3_18762"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.472"/>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009165">
        <property type="term" value="P:nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00583_B">
        <property type="entry name" value="RibP_PPkinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037515">
        <property type="entry name" value="Rib-P_diPkinase_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="PTHR10210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran_N" evidence="7">
        <location>
            <begin position="10"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Ribose-5-phosphate binding" evidence="6">
        <location>
            <begin position="229"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="43"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="102"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="327" mass="35816" checksum="6EFA4E2B2EA5540A" modified="2012-04-18" version="1">MTNRYFDTKLKIFALSSNRSLAEKVAENVGLDLGQLSVNRFSDGEIQINIEESVRGDNVYIIQSTSAPVNDNLMELLILVDALRRASARTINVVIPYYGYARQDRKARSREPITAKLVANMLQNSGVSRVLALDLHAAQIQGFFDIPVDHLMGAPLLADYFIQNGIAEEAVVVSPDHGGVTRARALAEFLKSPIAIIDKRRPKANIAQIMNIIGDVKGKKCIMLDDMIDTAGTITLGAQALMDAGAKEVYVSCTHAVLSGPAIERLENSPIKQVVVTDSIKLPKEKQIDKIVQVSVAPLIASAIKRINENRAVSPLFHNRFTETSQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIQ8</accession>
    <name>H4GIQ8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">4-diphosphocytidyl-2-C-methyl-D-erythritol kinase</fullName>
            <shortName evidence="9">CMK</shortName>
            <ecNumber evidence="2 9">2.7.1.148</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ispE</name>
        <name evidence="12" type="ORF">PS3_11614</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>4-CDP-2-C-methyl-D-erythritol + ATP = 4-CDP-2-C-methyl-D-erythritol 2-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57823"/>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.148"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the GHMP kinase family. IspE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.148" evidence="2 9"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121942.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11614"/>
    <dbReference type="EnsemblBacteria" id="EHS87253">
        <property type="protein sequence ID" value="EHS87253"/>
        <property type="gene ID" value="PS3_11614"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.478"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00094"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050515">
        <property type="term" value="F:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00061">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004424">
        <property type="entry name" value="IspE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43527:SF2">
        <property type="entry name" value="PTHR43527:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010376">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00154">
        <property type="entry name" value="ispE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6 9 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="10">
        <location>
            <begin position="88"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP_kinases_C" evidence="11">
        <location>
            <begin position="211"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="283" mass="31240" checksum="5AAA7CDD9B104781" modified="2012-04-18" version="1">MIITEKAPAKINLALDTPMRYFDGLPQWNMVMNSVDLADYVTVETHPSPQTIKVYTDSGFLPNDQRNLAYQAAHILKSRFHQSDGVTIRIQKNIPVAAGLGGGSSDAAAVLRALNQAWHLDLSLAELAKLALSIDSDVPYCVYGHTAHVTGHGEQVTPLPNFPHYWVVIAKPKVSVSTPTILRQINYETIQHVPTNQLVTTIQGHNWAQACQYMGNCLETVTCQEYPEINKLKQKMLSLGADVSQMSGTGPTVFAICHAESRAKRIYNGLKGFCHEVYQVVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIS1</accession>
    <name>H4GIS1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ArpR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_10571</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121955.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10571"/>
    <dbReference type="EnsemblBacteria" id="EHS87266">
        <property type="protein sequence ID" value="EHS87266"/>
        <property type="gene ID" value="PS3_10571"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.491"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="106" mass="11880" checksum="EF637F0B803886E9" modified="2012-04-18" version="1">MGYEELIPKIYDWAKERGIDQADPRVEFMKMAEELGELSGAFNKEQRDKLIDSIGDLQIALLIFCRLVGVDHQAALNAAYQEIAKRTGKTTADGVFIKTSDLDTTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIT2</accession>
    <name>H4GIT2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Energy-coupling factor transporter ATP-binding protein EcfA2</fullName>
            <ecNumber evidence="6">3.6.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_21295</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Forms a stable energy-coupling factor (ECF) transporter complex composed of 2 membrane-embedded substrate-binding proteins (S component), 2 ATP-binding proteins (A component) and 2 transmembrane proteins (T component).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cell membrane</location>
            <topology evidence="2 6">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC transporter superfamily. Energy-coupling factor EcfA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.-" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121966.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21295"/>
    <dbReference type="EnsemblBacteria" id="EHS87162">
        <property type="protein sequence ID" value="EHS87162"/>
        <property type="gene ID" value="PS3_21295"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.511"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030946">
        <property type="entry name" value="EcfA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04521">
        <property type="entry name" value="ECF_ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="4"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="287" mass="31396" checksum="1DE06D2F25763312" modified="2012-04-18" version="1">MEAIRFKDLTYIYQANSVMAQPALTKITTTFKQGATTAIIGHTGSGKSTLMQLIDGLILPTAGSITLGDLTITNETPVKELGKLRRLVGYVFQFPEKQLFAESVLDDVKFGPLNQGLSDEEAQERAQAALEQLNFPLELLKHSPFELSGGQQRRVAIAGVLAMHPEILILDEPTVGLDPEGQAELLTLVRDLQAQGLTILIVTHQMEIVAQLADEVMVLNHGQLAFQGSSQDLFLQPDLLADNHLRLPKAIQLATNLGQRGITWDGLPLTTAQLADQIIQMWGDQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIU6</accession>
    <name>H4GIU6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">50S ribosomal protein L5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rplE</name>
        <name evidence="10" type="ORF">PS3_3778</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA and the P site tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121979.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3778"/>
    <dbReference type="EnsemblBacteria" id="EHS87176">
        <property type="protein sequence ID" value="EHS87176"/>
        <property type="gene ID" value="PS3_3778"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.528"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_B">
        <property type="entry name" value="Ribosomal_L5_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_L5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020930">
        <property type="entry name" value="Ribosomal_L5_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_L5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020929">
        <property type="entry name" value="Ribosomal_L5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_L5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="PTHR11994"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00358">
        <property type="entry name" value="RIBOSOMAL_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L5" evidence="8">
        <location>
            <begin position="25"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal_L5_C" evidence="9">
        <location>
            <begin position="86"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="180" mass="20063" checksum="6687B23458886014" modified="2012-04-18" version="1">MENRLKAMYESEVRPSLIEKFNYSSVMAAPKIEKVVLNMGVGDATTNSKNLDEAVEELGLISGQKPLITKAKKSIAGFRLREGMAIGAKVTLRGDRMYDFLDKLINVSLPRVRDFHGVSNKAFDGRGNYTLGIREQLIFPEIDYDNVKRVRGLDVVIVTTANSDEESHKLLAQLGMPFAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIX6</accession>
    <name>H4GIX6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphomethylpyrimidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_17502</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThiD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122009.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17502"/>
    <dbReference type="EnsemblBacteria" id="EHS87148">
        <property type="protein sequence ID" value="EHS87148"/>
        <property type="gene ID" value="PS3_17502"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.571"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045029">
        <property type="entry name" value="HMP/HMP-P_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PTHR20858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phos_pyr_kin" evidence="2">
        <location>
            <begin position="15"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="265" mass="28140" checksum="2746AC72868B9371" modified="2012-04-18" version="1">MVNEFPQALSIAGSDSGGGAGMQADLKTMQMRHVFATAVLVAITAQNTLGVQDSMPLPPAMIDAQFASLNDDLQIRACKTGMLADVPTVECVAANLQKYDFGPVIVDPVMIAKGGAPLLTDDAIETVKKHLLPLATLITPNLPEAERLTGMQVDSKEEMVTVGHQLQDLGAKNVLVKGGHFDQSGLAQDFVLLANDESFWMTSPRFDTIRTHGTGDTISACITAELAKGVPLETAIRTSKAYVTATIRDGIEVGHGHGPLNHWAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIX8</accession>
    <name>H4GIX8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Succinyl-diaminopimelate desuccinylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_21287</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122011.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21287"/>
    <dbReference type="EnsemblBacteria" id="EHS87150">
        <property type="protein sequence ID" value="EHS87150"/>
        <property type="gene ID" value="PS3_21287"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.573"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010182">
        <property type="entry name" value="ArgE/DapE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01910">
        <property type="entry name" value="DapE-ArgE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="3">
        <location>
            <begin position="174"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="383" mass="41821" checksum="DC2002E3C941FFD1" modified="2012-04-18" version="1">MKPTQQLQILKDIIGIPSENQNEAAVADYIEQLFTPFSNVQIDRLNYAPGRDNIMVTVGNGQPTLVLTGHMDTVKAGDLKKWTSDPFQAEVRNGKLYGRGASDMKSGLAAIIVTLLEIVQSGQPVTGTIKLIGTVGEETGEYGAAQVTKAGWLDGADAVIIAEPNSGFHEITYTAKGVIDYRVTSKGKSAHSARPHLGINAIDKLLLFYQRMQTAMQQFDQVDPVLGEVVHNVTLIKGGEQVNTIPEDASLMGNIRTIPIYPNQMFYDVIEGLIEDLNQDPTTDLKLEYSFPEEAVPGRADSPLMKLAQTVYEDLFGFISEPVGAVSANEAAEFVHAQGDFDIIDFGPGSETSHAIDEYVPVSDYQKAIAFYHAFIQAFFTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIZ2</accession>
    <name>H4GIZ2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CN hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_16388</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122025.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16388"/>
    <dbReference type="EnsemblBacteria" id="EHS87104">
        <property type="protein sequence ID" value="EHS87104"/>
        <property type="gene ID" value="PS3_16388"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.596"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="259" mass="28657" checksum="14C652D5391A8B1F" modified="2012-04-18" version="1">MKVALAQIPIAYGDPAANLATVKEYVGKARAAGADTVVFPEMWNTGYALDQLSSLADPDGKNTQVLLGQLAQENQINLVGGSVATKEGNQFFNTTYVVNQTGELVGRYRKVHLFGLMNEDQYLTAGNQINRFELAGVPSSSFICYDLRFPEWWRTVNQTGVQVIYLPAEWPEKRIEQWELLVRARAIENQAFVVAVNTVGDNPTDHFGGHSMVVDPLGKVITKMDDQAGLEVVEFDPQAALAVRGPIPVFKDRRPNLYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ08</accession>
    <name>H4GJ08_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_21290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122041.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21290"/>
    <dbReference type="EnsemblBacteria" id="EHS87120">
        <property type="protein sequence ID" value="EHS87120"/>
        <property type="gene ID" value="PS3_21290"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.625"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="5"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="131" mass="15100" checksum="9D8F202A06126D72" modified="2012-04-18" version="1">MKMRRIDHLVLTVSDLEACKRFYHEVFDMPVIDDQTNDDVVTLRCGHQLIRLQKTDRANPLVAKHPTVGAADFCMIVGDQLEDVLHHLKSYYVEVITNEPIVKHGSEGEMRSIYLYDPDQNLLEISTYANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ38</accession>
    <name>H4GJ38_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Maltose phosphorylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_13415</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 65 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122071.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13415"/>
    <dbReference type="EnsemblBacteria" id="EHS87063">
        <property type="protein sequence ID" value="EHS87063"/>
        <property type="gene ID" value="PS3_13415"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.677"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901575">
        <property type="term" value="P:organic substance catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005194">
        <property type="entry name" value="Glyco_hydro_65_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005195">
        <property type="entry name" value="Glyco_hydro_65_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005196">
        <property type="entry name" value="Glyco_hydro_65_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037018">
        <property type="entry name" value="Glyco_hydro_65_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017045">
        <property type="entry name" value="Malt_Pase/Glycosyl_Hdrlase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03633">
        <property type="entry name" value="Glyco_hydro_65C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03632">
        <property type="entry name" value="Glyco_hydro_65m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03636">
        <property type="entry name" value="Glyco_hydro_65N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036289">
        <property type="entry name" value="Glycosyl_hydrolase_malt_phosph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_65N" evidence="6">
        <location>
            <begin position="13"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_65m" evidence="4">
        <location>
            <begin position="322"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_hydro_65C" evidence="5">
        <location>
            <begin position="699"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="357"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="591"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR036289-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR036289-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="750" mass="85425" checksum="26E1472E7247C69C" modified="2012-04-18" version="1">MKRTFEVAPWHVTTTQWDPENKRLQESMTSLGNENLGMRGFYEEGYSGDTLEGLYLGGVWYPDKTRVGWWKNGYPKYFGKVINAVNFIKLPIKVNGEQLDLAKQTPSNFKLDLDMQAAKLTRSFTAEVGGAQVEFNFERFISAATKELVGQRLTVKNLGDQAAEVEVSSLIDADVRNEDANYDEQFWNVLNTATDGDRALMTAITKENNFGTERFTVGFESSVKTSMDQVDTVKTEQQVGLVYRQSLAAGTELTFEKRSVFVSSRDFADEEAIVDRLHALTSELDQANFDDLLASHTAEWADRWTKSDVKIAGDDEAQQGIRFNLFQLFSTYYGNDSRLNIGPKGFTGEKYGGATYWDTEAFAIPVYLGVADPAVTRNLLMYRYNQLDGAFVNAQEQGLKGALYPMVTFNGIECHNEWEITFEEIHRNGDIAFAIYNYTRYTGDKSYVLHEGSKVLTEISRFWADRVHYSRRVGKYMLAGVTGPDEYQNNVDNNWYTNLLAQWTLKYTLEVLAEVDSDVAQDLQVSDEEKAHWQEIVDQMYLPEDQELGIFVENDGFLNKDLKPVSELADSDLPLNQNWSWDKILRSPYIKQGDVLQGIWDFIDDFTPEQKAKNFDFYEPLTVHESSLSASIHSILAADLHKEDQAVEMYERTSRLDLDNYNNDTVDGLHITSMTGSWLAIVQGFAGMRIRNDQLHYAPFLPKKWDSYQFRQQFRGRVIEVTVDANGTKLDLISGEPLTIDLDGQTVDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ54</accession>
    <name>H4GJ54_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Adenine phosphoribosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_6920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6920"/>
    <dbReference type="EnsemblBacteria" id="EHS87079">
        <property type="protein sequence ID" value="EHS87079"/>
        <property type="gene ID" value="PS3_6920"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.698"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="1">
        <location>
            <begin position="45"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="186" mass="20571" checksum="3ECF50903A2172BC" modified="2012-04-18" version="1">MMDYQISIGKFKRKLPLVKIDENTQIASFVLLGDTELTVEAARQLKEKLTLDYDFVVTLESKGIPLAHELTRQTNRDRYVVIRKSIKAYMTEPVVTTVESITTSEPQQLVLDGMDAAMLSGKRVVLLDDVISTGGSLKAAEELLARVDCQVVAKAAILAEGAATQRNDIIYLASLPLFDSRGRIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ65</accession>
    <name>H4GJ65_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_15867</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122098.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15867"/>
    <dbReference type="EnsemblBacteria" id="EHS87090">
        <property type="protein sequence ID" value="EHS87090"/>
        <property type="gene ID" value="PS3_15867"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.712"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011697">
        <property type="entry name" value="Peptidase_C26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044668">
        <property type="entry name" value="PuuD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43235">
        <property type="entry name" value="PTHR43235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07722">
        <property type="entry name" value="Peptidase_C26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="243" mass="27215" checksum="CE75BC494108FBD7" modified="2012-04-18" version="1">MVKPIIGITGGVLTNAGPFQGYHRSYVNEDYVQAVIANGGVPVIIPVTSNEEVIKSQINTVDAVILSGGNDIDPRNYHENTTQESGDLMPSRDWFDLQVVQLAERANKPILGICRGAQIINVAHGGSLYQDLKYRQHTSLQHLYHDHPDQVTQTISINTGSKLAHIFDQTKLAINSFHHQAIHRLGRHLQATAWAEDGVIEGFESPQQAIIGVQWHPEMLYRREKTMDRLFQALINQAQGERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ66</accession>
    <name>H4GJ66_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyrroline-5-carboxylate reductase</fullName>
            <shortName evidence="2">P5C reductase</shortName>
            <shortName evidence="2">P5CR</shortName>
            <ecNumber evidence="2">1.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">PCA reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">proC</name>
        <name evidence="5" type="ORF">PS3_17133</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-proline + NAD(+) = 1-pyrroline-5-carboxylate + 2 H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15893"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-proline + NADP(+) = 1-pyrroline-5-carboxylate + 2 H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15893"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122099.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17133"/>
    <dbReference type="EnsemblBacteria" id="EHS87021">
        <property type="protein sequence ID" value="EHS87021"/>
        <property type="gene ID" value="PS3_17133"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.713"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PTHR11645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00521">
        <property type="entry name" value="P5CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_oxidored" evidence="3">
        <location>
            <begin position="3"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="P5CR_dimer" evidence="4">
        <location>
            <begin position="157"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="256" mass="26344" checksum="B25964F093B68E15" modified="2012-04-18" version="1">MTKVAVVGTGSMGSAILEGLAKTKQVDLVAANHPNPRAKQLADQLKIKLYERNQDLIAEQPDVVILTVPVPVTLDVAKELAGLTAGTVVMSASVGVTASELQAILPQAGVVPFVPNTPVAINAGTIGVAFNDQLSADQRQLANQVLALLGDVISVPEERMDIVGTVGGCGPAFVDVMIDAMSDAAVAKGLDRATAYQLIASMVKGSGALASQSDLTPSQLRDQVTSPAGTTIRGVMALEANGFRHAVIEAINKSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ96</accession>
    <name>H4GJ96_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lysozyme</fullName>
            <ecNumber evidence="4">3.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_19898</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Hydrolysis of (1-&gt;4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.</text>
            <dbReference type="EC" id="3.2.1.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.17" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000031">
        <property type="protein sequence ID" value="EHS87008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19898"/>
    <dbReference type="EnsemblBacteria" id="EHS87008">
        <property type="protein sequence ID" value="EHS87008"/>
        <property type="gene ID" value="PS3_19898"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.765"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003796">
        <property type="term" value="F:lysozyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009253">
        <property type="term" value="P:peptidoglycan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002053">
        <property type="entry name" value="Glyco_hydro_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008270">
        <property type="entry name" value="Glyco_hydro_25_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018077">
        <property type="entry name" value="Glyco_hydro_fam25_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01183">
        <property type="entry name" value="Glyco_hydro_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00641">
        <property type="entry name" value="Glyco_25"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00953">
        <property type="entry name" value="GLYCOSYL_HYDROL_F25_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51904">
        <property type="entry name" value="GLYCOSYL_HYDROL_F25_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="211" mass="22529" checksum="C95AB2353FB858CC" modified="2012-04-18" version="1">MQGGIQTGDYGVDVSSFQGTDLSGYAANGAKYAIVKVTEGTGYINYNASGQIASAQANNLYTMAYHFATFSNDSYAAVNQANFAVQQAQAAGLPAGSYLCLDWESGDGNYIYGNTEANTQAILAFMRTVQAAGYKTMLYSGASAFRNNVNISEVLAEFPNSLWVASYPTTSAQWQADMNYFPSMNGVAIWQFADNWAGFGVDGNIAVTNVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ99</accession>
    <name>H4GJ99_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phage tape measure protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000031">
        <property type="protein sequence ID" value="EHS87011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13784"/>
    <dbReference type="EnsemblBacteria" id="EHS87011">
        <property type="protein sequence ID" value="EHS87011"/>
        <property type="gene ID" value="PS3_13784"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.775"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="487" mass="52408" checksum="F6DC87C9D3F4447E" modified="2012-04-18" version="1">MAANPMIMTIAIIAAVVSALAVFFTQTTIGKKIWKGFTDWFGQVWRSLSSIITPIFTEIKNNIANAVPIIQAAWQGFMTWFQGIWQTVGPVVSAVWNGITTAVSTVITTIQTAWQGFVTWFQGIWNSISPVVVPVIQAIVGIIATMFTSLQGLWTMLQPAFSSIGTALSTLAPLFSALWGYIQQLWTLIQPIVQYLISTLGPALAVVIVGAIMAVITSLTSLSIIVSTIVGVIQATVATTVATFSIAFTIISGVIQTFAVIFQTVGQIIVAVFTGNWSAIPGIVKGGWNKVSRIFSNTVGSLNKIVANWGNSLIQAGKSIMDGFLNGLKAGWNAVTKFIGGIKDWIVKHKGPISDDAVALIPAGQAFMQGLNAGLLSGFTDVQDNVMQMSGWINDAINQSIDTINGFKSADNMSVNNDLNRTITSQSITDVRADNRYGNLEALVQTAVNKLDNIDQTPVVTVDTLNTMNQYQNGVNAKNYSMIKGRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJC2</accession>
    <name>H4GJC2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Biotin carboxyl carrier protein of acetyl-CoA carboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_10410</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 4">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000033">
        <property type="protein sequence ID" value="EHS86991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122155.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000033.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10410"/>
    <dbReference type="EnsemblBacteria" id="EHS86991">
        <property type="protein sequence ID" value="EHS86991"/>
        <property type="gene ID" value="PS3_10410"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.823"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009317">
        <property type="term" value="C:acetyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001249">
        <property type="entry name" value="AcCoA_biotinCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01071">
        <property type="entry name" value="ACOABIOTINCC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0092">Biotin</keyword>
    <keyword evidence="4" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="5">
        <location>
            <begin position="68"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS86991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="144" mass="15537" checksum="2AB7AB1328B7FA26" modified="2012-04-18" version="1">MKIDQAAIEALAQIMQDQGLGEIEYQDGDQRIKLVSATTAENTLTDPRSLNGPELTQPVRAQTANVDGATINAPTVGIFYTAKSPQDPPYVQVGDHVEPGQIVGVIEAMKMFTNVMADQAGTVERVLVNNEEGVEYGQPLIQIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJD2</accession>
    <name>H4GJD2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">DNA repair protein RecN</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 6">Recombination protein N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_13779</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">May be involved in recombinational repair of damaged DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the RecN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000035">
        <property type="protein sequence ID" value="EHS86881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122165.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000035.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13779"/>
    <dbReference type="EnsemblBacteria" id="EHS86881">
        <property type="protein sequence ID" value="EHS86881"/>
        <property type="gene ID" value="PS3_13779"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.839"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004604">
        <property type="entry name" value="DNA_recomb/repair_RecN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11059">
        <property type="entry name" value="PTHR11059"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003128">
        <property type="entry name" value="RecN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00634">
        <property type="entry name" value="recN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC_N" evidence="7">
        <location>
            <begin position="1"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR003128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="562" mass="63183" checksum="5A8996D61BAF8D1E" modified="2012-04-18" version="1">MIQELTIDNLAIIDHLTLTFADHMTVLSGETGAGKSIIIDAVGLLAGGRGSQEFIRRGAEKLSLQGQFTLPTDTAMLDQLDQLGIPYDESGLIISREIHRNGRNAIRINGTLVNTTILKQVGRGLVDIQGQNEHQQLMQPESHLGMLDQYAQQEVDEIKQQYHTLYQQYRHLSERIKAKKANEQQWAQRLDMLRYQVNEIEEANLSPNEEEELRQERERLSHFQQINDALQTVVGMLNESEPVPVLDQLGASLQDLAAIATYDQDYQDLSKTFDEAYYSLQDVAAQASRQLDALEYDPRRLSEIDDRLTQIGNLEHKYGSTINDILTYYDQISQELSTMEEAAHSDEDLDDQIAHLATQLTKLGQKLSQIRQQAAERLAQLVDQQLHDLYMEKAIFKVHFDQVPAGQFLATGIDEVEFYIQTNQGESMGPLARIASGGELSRVMLALKTVFASAEGVTSIIFDEVDTGVSGRVAQAIADKIRLIAQHSQVLCITHLPQVAAVAQHHFLIQKEVHDQRTITTVSTLNEQQRVEELARTLAGETVTELALEHAQELLEMAKEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJE4</accession>
    <name>H4GJE4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rplU</name>
        <name evidence="6" type="ORF">PS3_8095</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">This protein binds to 23S rRNA in the presence of protein L20.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Contacts protein L20.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the bacterial ribosomal protein bL21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000035">
        <property type="protein sequence ID" value="EHS86893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122177.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000035.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8095"/>
    <dbReference type="EnsemblBacteria" id="EHS86893">
        <property type="protein sequence ID" value="EHS86893"/>
        <property type="gene ID" value="PS3_8095"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.854"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01363">
        <property type="entry name" value="Ribosomal_L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036164">
        <property type="entry name" value="L21-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028909">
        <property type="entry name" value="L21p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001787">
        <property type="entry name" value="Ribosomal_L21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018258">
        <property type="entry name" value="Ribosomal_L21_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349">
        <property type="entry name" value="PTHR21349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00829">
        <property type="entry name" value="Ribosomal_L21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141091">
        <property type="entry name" value="SSF141091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00061">
        <property type="entry name" value="L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01169">
        <property type="entry name" value="RIBOSOMAL_L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS86893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="102" mass="11172" checksum="048E080E34F1EC22" modified="2012-04-18" version="1">MYAIIVTGGKQYKVEAGQAVYVEKLNAEAGDKVTFDKVVFVGGDDTKIGTPFVDGATVEGTVDKQGKEKKVVTFKYKPKKHTHNKKGHRQPYTKVTIDAINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJF7</accession>
    <name>H4GJF7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Prophage Lp2 protein 17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_16983</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000036">
        <property type="protein sequence ID" value="EHS86880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122190.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000036.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS86880">
        <property type="protein sequence ID" value="EHS86880"/>
        <property type="gene ID" value="PS3_16983"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.903"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006505">
        <property type="entry name" value="Phage_nucleotide-bp"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01618">
        <property type="entry name" value="phage_P_loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="213" mass="24204" checksum="9996951E33E0427F" modified="2012-04-18" version="1">MRFYESGKIPRQSNMYFIYGDGGTGKTSLFKQFPGKKLLISFDMSTNPLVGDDSIDVAILDESEKPRIQSVLNEFLIRAISSGRYQSICLDNVSALQNYVLENIDGQSKDGRQNYQKLQLWFRDLGSYLRNVNINVYVTAHSIDKGADVVNGRYEADMNVKTFNALTSMFDVVGRIYLQDGQRMIDLDPEQGNHAKNRIDGRKLIKADELLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJH7</accession>
    <name>H4GJH7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Manganese transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_8608</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000039">
        <property type="protein sequence ID" value="EHS86620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8608"/>
    <dbReference type="EnsemblBacteria" id="EHS86620">
        <property type="protein sequence ID" value="EHS86620"/>
        <property type="gene ID" value="PS3_8608"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.931"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="PTHR11706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01197">
        <property type="entry name" value="nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="390"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="428"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="458"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="496"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS86620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="537" mass="58508" checksum="5BC1CC838D74A8E1" modified="2012-04-18" version="1">MRTRQFNIRKGGFDVKNTQNKLRAPKLISHTNGKSLEEINGTVEVPRGKGFLKTLLAYSGPGALVAVGYMDPGNWSTSITGGQSFQYTLMTTILISSLIAMLLQAMAAKLGIVSEMDLAQAIRARTGKALGVILWIMTELAIMATDIAEVIGAAIALNLLFHIPIIWAVMITVLDVMVLLLLTKIGFRKIEAIVACLILVILFVFLYEVILSNPNWGGVIAGLVPTPEAIAQHPVVNGISPLSGSLGIIGATVMPHNLYLHSAISQTRKIDHTDANDIKQTVRFTTWDSNIQLSLAFVVNSLLLIVGVAVFKSGAVSDTSFFGLYEALNNTSMLSNPILISVAKSGVLSTLFAVALLASGQNSTITGTLTGQVIMEGFIHLKMPLWARRLVTRLLSVVPVVLCVLMTAGETEIQQHQAINMLMENSQVFLAFALPFSMLPLLLMTNSKAEMGQFKNSWITQILGWISVIGLTFLNLYNLPSTFEGFGIWNKSLSDILAWITIILILLLLAWTTIDIIRGDRRLAANRVAHPWETTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJH9</accession>
    <name>H4GJH9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoenolpyruvate carboxykinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_13003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000041">
        <property type="protein sequence ID" value="EHS86569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122212.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000041.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13003"/>
    <dbReference type="EnsemblBacteria" id="EHS86569">
        <property type="protein sequence ID" value="EHS86569"/>
        <property type="gene ID" value="PS3_13003"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.936"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004612">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001272">
        <property type="entry name" value="PEP_carboxykinase_ATP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01293">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="562" mass="64220" checksum="2FD59DCDB999513C" modified="2012-04-18" version="1">MAQESTIRTKSLYVNPERRILVMNLNEQYFTGVNELVDSDEFKELVTLYLKTDNLFNTYEDYRNLTADQFIKMTKRILAHDETVFANLDRQILLSCYEDAYTYFRNFLRVTVTDGMNKVLVNSFLDIDDRLNDLFRTCYRTLTDEVRGRRVNRYRQISSASNAALLTKHHQWPIPAGYEALDKINFIDMAMVRPPLMMHTPSNKREGVFSPVTENPVKKFARQKGEWYCYPAKIGTSIAYIYFHEDFMVSGLSLTNLFAMAHDYEVEGRKPDLILFFGLDKTEGDVSHYFYDEANDIWVGEVPNTVKTTYFGYMKKMCLTLHNLHQIKQGKLPIHGSMVRIAFANGKTKNVVFFGDSGAGKSESLEALQEIADEQIVDMETIFDDMGSFTIDGDKVYAQGTETGAFVRLDDLSSAVAFNNMDRGIYLNPELKNARVIIPVTSIENVIAHHHVDMWVYANNYDAEVGIHQFENETAAKEVFVAGKRKALGTTDESGMTTTFFANPFGPVQEPELTQPIIDDVFKKFFDAGIYVGEIYTHLGYDRSAEALHDSARAMLKVLMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJJ4</accession>
    <name>H4GJJ4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GTP pyrophosphokinase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_7521</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122227.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7521"/>
    <dbReference type="EnsemblBacteria" id="EHS86504">
        <property type="protein sequence ID" value="EHS86504"/>
        <property type="gene ID" value="PS3_7521"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.963"/>
    <dbReference type="UniPathway" id="UPA00908">
        <property type="reaction ID" value="UER00884"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015970">
        <property type="term" value="P:guanosine tetraphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05399">
        <property type="entry name" value="NT_Rel-Spo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007685">
        <property type="entry name" value="RelA_SpoT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04607">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00954">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RelA_SpoT" evidence="2">
        <location>
            <begin position="39"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="199" mass="22889" checksum="50BA0B8654F59156" modified="2012-04-18" version="1">MSIYGEQGQYLPMILTEIEDRLQEINASSSPKLYEHLIGRVKAEDSMIEKCHRKGFPATPQGALRDCRDAIALRLVCNFIDDVYRAIEALRQADWCQIVTEKDYITNAKPNGYRSYHLILDVTTPYPDVDQQTPGHYFVEIQLRTIAMDSWASLEHELKYKHNIKDAERIAGELKRCAEQLAACDVQMQTIRQLINEAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJK6</accession>
    <name>H4GJK6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTPase HflX</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GTP-binding protein HflX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hflX</name>
        <name evidence="9" type="ORF">PS3_6496</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">May associate with membranes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. HflX GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122239.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6496"/>
    <dbReference type="EnsemblBacteria" id="EHS86516">
        <property type="protein sequence ID" value="EHS86516"/>
        <property type="gene ID" value="PS3_6496"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.980"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01878">
        <property type="entry name" value="HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00900">
        <property type="entry name" value="GTPase_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030394">
        <property type="entry name" value="G_HFLX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032305">
        <property type="entry name" value="GTP-bd_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016496">
        <property type="entry name" value="GTPase_HflX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025121">
        <property type="entry name" value="GTPase_HflX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042108">
        <property type="entry name" value="GTPase_HflX_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229">
        <property type="entry name" value="PTHR10229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16360">
        <property type="entry name" value="GTP-bdg_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13167">
        <property type="entry name" value="GTP-bdg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006809">
        <property type="entry name" value="GTP-binding_hflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03156">
        <property type="entry name" value="GTP_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51705">
        <property type="entry name" value="G_HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hflx-type G" evidence="8">
        <location>
            <begin position="202"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="208"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="240"/>
            <end position="244"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="262"/>
            <end position="265"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="328"/>
            <end position="331"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="349"/>
            <end position="351"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS86516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="425" mass="47824" checksum="A1B43A35AE473428" modified="2012-04-18" version="1">METTKPNLDQIIIIGLDTGQSNFDYTMNELVELAKANNMQVLDRLDQALDRPNAATYFGKGKVEELANLTAALKATTIVANDELSPSQIKNLTDATKTRVIDRTALILEIFAQRAQSREAKIQVEIAQLQYRLPRLRTANNITLDQQSGGGAGMSNRGAGETQMEMDRRVIQRHISHLRNELKEIQKSEDTKRSQRDKSAIPTAALVGYTNAGKSTIMNQLVERYGVSVDKQVFEKDMLFATLDTSVRQLTLKDQKRFLLSDTVGFVSKLPTQLVEAFKSTLAEAANADLLIQVIDYSDPHYEEMIKTTESTLHQIGIEDIPMVYVFNKADRTAVEYPVMEGEDRIVISAKDAASIDLLEQIIRQHLFKDYQTATLLVPFAEGNVVSYLNEHTNILETTYEEAGTRLKVELSSEDLHRFENYLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJL2</accession>
    <name>H4GJL2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_2961</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122245.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2961"/>
    <dbReference type="EnsemblBacteria" id="EHS86522">
        <property type="protein sequence ID" value="EHS86522"/>
        <property type="gene ID" value="PS3_2961"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.990"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="140" mass="15747" checksum="998DAB240316F070" modified="2012-04-18" version="1">MATEITSGAVVYRRTATGIEYLLLQSQNPGHFWGFPKGHVEAGEDLVTAATREIREETSLHLKIDSSFQVATSYDLSNGNLKEMTLYTAPVPTDTELNLQTEEIANAGWFNYQAARQRLTYDNLKSLLDQVNQHLVELEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJL6</accession>
    <name>H4GJL6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Proline iminopeptidase</fullName>
            <shortName evidence="9">PIP</shortName>
            <ecNumber evidence="4 9">3.4.11.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Prolyl aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PS3_2778</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Releases the N-terminal proline from various substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Release of N-terminal proline from a peptide.</text>
            <dbReference type="EC" id="3.4.11.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the peptidase S33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.5" evidence="4 9"/>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122249.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2778"/>
    <dbReference type="EnsemblBacteria" id="EHS86526">
        <property type="protein sequence ID" value="EHS86526"/>
        <property type="gene ID" value="PS3_2778"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.994"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031975">
        <property type="term" value="C:envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002410">
        <property type="entry name" value="Peptidase_S33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005945">
        <property type="entry name" value="Pro_imino_pep"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005539">
        <property type="entry name" value="Pept_S33_TRI_F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00793">
        <property type="entry name" value="PROAMNOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01250">
        <property type="entry name" value="pro_imino_pep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="11">
        <location>
            <begin position="27"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS86526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="301" mass="34573" checksum="63306D7CCD628C38" modified="2012-04-18" version="1">MKTGTTIITLDNGYHLWTNTQGSGTIHLLCLHGGPGGNHEYWEDTAAQLKAQGLDVQVHMYDQLGSLYSDQPDYSDPANRDQFLTYEYFLDEVEEVRQKLGIDQFYLIGQSWGGLLVQEYAVKYGEHLKGAIISSMVDEIDEYVEHVNQVREETLPAEEVAFMKECERTNNYDNERYQKNVQILNEGFVDRKQPSKLYHLMDLGGTATYNAFQGDNEFVITGKLKDWHFRDQLPKIKVPTLLTFGEHETMPLATARTMADIIPHARLVTTPDGGHHHMVDNPDVYYRHLADFIRDVENDHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJN3</accession>
    <name>H4GJN3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CRISPR-associated endonuclease Cas1</fullName>
            <ecNumber evidence="8">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cas1</name>
        <name evidence="9" type="ORF">PS3_13008</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer, forms a heterotetramer with a Cas2 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CRISPR-associated endonuclease Cas1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000044">
        <property type="protein sequence ID" value="EHS86256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122266.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000044.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13008"/>
    <dbReference type="EnsemblBacteria" id="EHS86256">
        <property type="protein sequence ID" value="EHS86256"/>
        <property type="gene ID" value="PS3_13008"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1022"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01470">
        <property type="entry name" value="Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002729">
        <property type="entry name" value="CRISPR-assoc_Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042206">
        <property type="entry name" value="CRISPR-assoc_Cas1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042211">
        <property type="entry name" value="CRISPR-assoc_Cas1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019855">
        <property type="entry name" value="CRISPR-assoc_Cas1_NMENI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01867">
        <property type="entry name" value="Cas_Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00287">
        <property type="entry name" value="cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03639">
        <property type="entry name" value="cas1_NMENI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS86256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="301" mass="34833" checksum="709A11757C0F9FE9" modified="2012-04-18" version="1">MGWRSVVITQHAKISYSANMIIVQTRDGINQIPVSDIDVLLIDTTQAVITTAVIAELANRETKIIFTDKRHQPITESVNYYPNNRSAEMLLRQVNWDQNREDILWTKIVSYKIRNQIKVLEVYNQPTDEIESELDKLEVGDLSNREAVIARKYFPLLFEDKFSRRDESSVNAALNYGYSILLSMMNKEIVTRGNLTYLGIHHHSSENEFNLGSDSMEPFRPVIDYWVAAQKFNEFTPDVKYGLVDALNLEIIYNGQKMLLRNVIPKYVQTCLDYLDENRDELEIEVEFTNEVPHNALNGHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJN9</accession>
    <name>H4GJN9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal RNA small subunit methyltransferase G</fullName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">16S rRNA 7-methylguanosine methyltransferase</fullName>
            <shortName evidence="2">16S rRNA m7G methyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rsmG</name>
        <name evidence="3" type="ORF">PS3_5968</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically methylates the N7 position of a guanine in 16S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000045">
        <property type="protein sequence ID" value="EHS86240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5968"/>
    <dbReference type="EnsemblBacteria" id="EHS86240">
        <property type="protein sequence ID" value="EHS86240"/>
        <property type="gene ID" value="PS3_5968"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1028"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070043">
        <property type="term" value="F:rRNA (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00074">
        <property type="entry name" value="16SrRNA_methyltr_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003682">
        <property type="entry name" value="rRNA_ssu_MeTfrase_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31760">
        <property type="entry name" value="PTHR31760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02527">
        <property type="entry name" value="GidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003078">
        <property type="entry name" value="GidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00138">
        <property type="entry name" value="rsmG_gidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="2">
        <location>
            <begin position="138"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="253" mass="28203" checksum="F6043AF09C021E49" modified="2012-04-18" version="1">MFHGKEPLMTPEEFRQSLLDQGITVTDQQLAQFDYYYHFLVETNEHVNLTAITDREEVYLKHFYDSILGAIAQPKLQTEAWTLCDIGAGAGFPSLPLKILFPQLKVTIVDSLNKRIKFLQDLCDQLGLTEVTLVHDRAETFSAKNSPYREQYDLVTARAVARLVVLSELCLPAVKVGGQFMAYKATAAQDELQVAQGATNKLGGQLTDVKTLSLPSQPAEERNFVLINKVAATPGKYPRRPGVPNKKPLVNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJS3</accession>
    <name>H4GJS3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_21033</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS86110">
        <property type="protein sequence ID" value="EHS86110"/>
        <property type="gene ID" value="PS3_21033"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1091"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="362"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="7"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS86110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="392" mass="42705" checksum="ABD1FB0180ECB39C" modified="2012-04-18" version="1">MGQRRRLSLSLYLNYVVHGFGLLILAQNMTNLASSWGVTIKMVSFIISGVGIGRLLAYLITGYWADRLSRKLFVYLGMACYFVFAVGIVMSPSIPVAYVLAILAGIANSALDAGTYTTFVEMNNGNGQFNVLLRAVVSVGEFILPLLVSFFSHDGRWYGWTFLIMAGLLAINAVLLMPIRFPKRPEVTSSSNQDSQGSRNNPGRLIASGAFVIYGYVSMALMIWFTQWVTLFGENSQHYTASQSHWLLSLYSIGSILGVLTLFILLGRNVGEAKIIIAMNLIATGSLIMLVLSHQLVVAEITSFIFGFSAASGVMQTGLTLFMKLYPQHRGMVTGVFYFFGAIASFTVPIITGILSDISIQMAFAGDIVIGIVAVLMAIIIALATRNIKFVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJT6</accession>
    <name>H4GJT6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycerol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_21279</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122319.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21279"/>
    <dbReference type="EnsemblBacteria" id="EHS86123">
        <property type="protein sequence ID" value="EHS86123"/>
        <property type="gene ID" value="PS3_21279"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1113"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016205">
        <property type="entry name" value="Glycerol_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616">
        <property type="entry name" value="PTHR43616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000112">
        <property type="entry name" value="Glycerol_dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Fe-ADH" evidence="5">
        <location>
            <begin position="7"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="92"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="114"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS86123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="363" mass="40178" checksum="01DD31B2C8C9B211" modified="2012-04-18" version="1">MFSQSGPQRYISDETAYQVLPDYFHELGLKTVVFLHGEKGYAVAKSFLPKWPADVSVIDVPFNGECSYEEIDRVKKICADSKADAIMALGGGKCLDTAKSVANGTKLAQIMLPTLASNCAAWSALSVHYRENHEHIDHKIYLRTSELLLVNPKVIFNSPLDYFVAVIGDTLAKFYESERVFEHLKPEEFTDALIISQRMAKQCQEILLKYGVQAVEDMKKGNYNENWRQVAETIIVTAGTVGGWGDAYARATAAHPVCDALTAFPRTHNHLHGAKVAYGIFVMLALENQEDDLKDLLPFYRQIGLPTNLADLDFGDATDAELDQLVADITAPNTQLHLLPATDISPAAVHHAITRVEELTKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJV6</accession>
    <name>H4GJV6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Guanylate kinase</fullName>
            <ecNumber evidence="2 9">2.7.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">GMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gmk</name>
        <name evidence="11" type="ORF">PS3_3361</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential for recycling GMP and indirectly, cGMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + GMP = ADP + GDP</text>
            <dbReference type="Rhea" id="RHEA:20780"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the guanylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="2 9"/>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122339.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3361"/>
    <dbReference type="EnsemblBacteria" id="EHS86143">
        <property type="protein sequence ID" value="EHS86143"/>
        <property type="gene ID" value="PS3_3361"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1134"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:guanylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00328">
        <property type="entry name" value="Guanylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="10">
        <location>
            <begin position="5"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="12"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS86143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="206" mass="23581" checksum="B72248D758A9C9BA" modified="2012-04-18" version="1">MAKRGVLIVLSGPSGVGKGTVRKALFDQPGNDFQYSISMTTRQPREGEVNGKDYFFVTKEEFEHEIQTGGMLEYAKYVDNYYGTPLKYVNETLESGKDVFLEIEVNGAMQVRSRTSDGVFIFLTPPDIMELKHRLIGRGTESMDVIDERIKTAFDEIRMMQNYDYAVVNDEVSNAVERIKEIIRTEHLRVNRVMPDYLEMLGEANQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJW6</accession>
    <name>H4GJW6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative galactoside O-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_11222</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000050">
        <property type="protein sequence ID" value="EHS86040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122349.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000050.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11222"/>
    <dbReference type="EnsemblBacteria" id="EHS86040">
        <property type="protein sequence ID" value="EHS86040"/>
        <property type="gene ID" value="PS3_11222"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1151"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="184" mass="20419" checksum="AC9ADFF1C95A467E" modified="2012-04-18" version="1">MDIFERFRNGEIIDLRDPQYVKEALTEMQRCRHLCWKINQTDPDKVNQITTLEKELVINQRDNCFITPPFQIDMGCCLHLGKNVFINSGLTMMSIGSIFIDDGTMIGPDVGFFTTNHDPHDVGKMSTKEIHIGKNVWIGARVNILPGVTIGDGAVIGTGTVVTKDIPAHSIAVGNPARVIKTIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJX4</accession>
    <name>H4GJX4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Large-conductance mechanosensitive channel</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mscL</name>
        <name evidence="12" type="ORF">PS3_10166</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homopentamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the MscL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS85991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122357.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10166"/>
    <dbReference type="EnsemblBacteria" id="EHS85991">
        <property type="protein sequence ID" value="EHS85991"/>
        <property type="gene ID" value="PS3_10166"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1165"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00115">
        <property type="entry name" value="MscL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019823">
        <property type="entry name" value="Mechanosensitive_channel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001185">
        <property type="entry name" value="MS_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037673">
        <property type="entry name" value="MSC/AndL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036019">
        <property type="entry name" value="MscL_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30266">
        <property type="entry name" value="PTHR30266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01741">
        <property type="entry name" value="MscL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01264">
        <property type="entry name" value="MECHCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81330">
        <property type="entry name" value="SSF81330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00220">
        <property type="entry name" value="mscL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01327">
        <property type="entry name" value="MSCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="93"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS85991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="136" mass="15027" checksum="893BD657EF85EB66" modified="2012-04-18" version="1">MLKEFREFILRGNVMDLAVGVIIGSAFTAIVTSLVKNLINPLIGIFLGRIDLSNLVFKVGDATFRYGAFLNSVINFLIIALVVFLLMKSINTVMEKAAALAKSEEDAKEAEAEEKEATETDYLKQIVALLEKQDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJY1</accession>
    <name>H4GJY1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutamine ABC transporter ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_19996</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS85998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122364.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19996"/>
    <dbReference type="EnsemblBacteria" id="EHS85998">
        <property type="protein sequence ID" value="EHS85998"/>
        <property type="gene ID" value="PS3_19996"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1177"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015424">
        <property type="term" value="F:ABC-type amino acid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030679">
        <property type="entry name" value="ABC_ATPase_HisP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039085">
        <property type="entry name" value="ABC_ATPase_HisP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="242" mass="26678" checksum="541C1B67FE3E932E" modified="2012-04-18" version="1">MLEIKNLQKKFGDHEVLKDITAEVDNGQVICVIGPSGSGKSTFLRCLNLLEEPTSGKVIFEGQELTNLKEAQLDVLREKMGMVFQSFNLFPNMTVLDNITLAPIKVKKIDKAKAEATAKELLDRVGLAEKANAYPASLSGGQQQRVAIARALAMNPDLMLFDEPTSALDPEMVGEVLEVMKDLAKSGMTMVVVTHEMGFAKEVADEVWFMDEGYIQEQGTPTEVFDHPTSERAQSFLAKILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJY5</accession>
    <name>H4GJY5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_10567</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS86002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122368.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10567"/>
    <dbReference type="EnsemblBacteria" id="EHS86002">
        <property type="protein sequence ID" value="EHS86002"/>
        <property type="gene ID" value="PS3_10567"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1186"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="278" mass="30024" checksum="7238F8169BE8BFCF" modified="2012-04-18" version="1">MKQEVMLVTGAGQLSMAIARRMGSGKKIILGDLSLDNAKNIAEVMTNAGFDVEAEQMDLSSRESIQNLIKVGQSFGDIKYLVNGAGVSPSQVPVETILKVDLYGTAVLMEEVRKVIVPGGSGVVISSQSGFRMPALTAEQDRALAMTPTEELLDLDFLQPDQIENTLHAYQLAKRANEKRVMYEAVKWGEKGARINDIAPGIIVTPLAIDEFNGIRGDFYKNMFAKSPAGRPGTADEVADVAELLMSERSQYITGSTVLIDGGATASYFYGPLQPTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK12</accession>
    <name>H4GK12_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CvpA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_8092</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000054">
        <property type="protein sequence ID" value="EHS85864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122395.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000054.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8092"/>
    <dbReference type="EnsemblBacteria" id="EHS85864">
        <property type="protein sequence ID" value="EHS85864"/>
        <property type="gene ID" value="PS3_8092"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1238"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009403">
        <property type="term" value="P:toxin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003825">
        <property type="entry name" value="Colicin-V_CvpA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02674">
        <property type="entry name" value="Colicin_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS85864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="176" mass="20066" checksum="C9DC1E82AEC0AEAD" modified="2012-04-18" version="1">MILTVIILAILIAAGLRGYRQGFVALMLSLVIYFVAWLLASWGANGFGSFLMQIFPQIGSDNAQTTLTSNLTLATNQLFYHGIAFFILFLIVNALGRWLIRRFHWVNRIPLIGWVNQWAGGIVNLVIAYLIIFILLVIFQVLPIEGWQNQLTASGLAQWIIHQTPFLTQWFFNWIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK22</accession>
    <name>H4GK22_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bifunctional purine biosynthesis protein PurH</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="8">Phosphoribosylaminoimidazolecarboxamide formyltransferase</fullName>
                <ecNumber evidence="8">2.1.2.3</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="8">AICAR transformylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="8">IMP cyclohydrolase</fullName>
                <ecNumber evidence="8">3.5.4.10</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="8">ATIC</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="8">IMP synthase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="8">Inosinicase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="8" type="primary">purH</name>
        <name evidence="10" type="ORF">PS3_8100</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(6S)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide = (6S)-5,6,7,8-tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:22192"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="2.1.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>H2O + IMP = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">The IMP cyclohydrolase activity resides in the N-terminal region.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PurH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.3" evidence="8"/>
    <dbReference type="EC" id="3.5.4.10" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000055">
        <property type="protein sequence ID" value="EHS85838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122405.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000055.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8100"/>
    <dbReference type="EnsemblBacteria" id="EHS85838">
        <property type="protein sequence ID" value="EHS85838"/>
        <property type="gene ID" value="PS3_8100"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1253"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00133"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00139">
        <property type="entry name" value="PurH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024051">
        <property type="entry name" value="AICAR_Tfase_dup_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="PTHR11692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000414">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00798">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00355">
        <property type="entry name" value="purH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MGS-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="512" mass="56557" checksum="25F1B4863ADD801B" modified="2012-04-18" version="1">MKRALVSVSDKTNLVPFVQGLVANGYEIISTGGTKKQLDDAGIPTIGIEDVTHFPEMLDGRVKTLNPYVHGGLLARRDLPEHMATLKEHQITPIDLVCVNLYPFKETISKPGVELADAIENIDIGGPSMVRSAAKNYQSVTIVVDQADYDQVLTEIKNDGDTTLETRFKLAAKAFRHTAAYDALISQYLTEQVGLEDPEKLTLTYDLKETMRYGENSHQKAWLYEDTMPKQFSILEAKQLHGKQLSYNNIKDADEALRCVREFDEPTVVAMKHMNPCGIGTAEELELAWDRAYEADPVSIFGGVIAVNRPVDLATAEKMRKIFLEIIVAPSFDDDAYEVLAKKKNIRLLQVNFDAKDEAMGHETVSVMGGLLVQEQDVLAEEPTGWEVVTKAKPTAEQLKSMAFAWKAVKHAKSNAIVVANNERTLGVGEGQPNRIDSLRIAVEHAGDAIDDRTVMASDAFFPFDDCVAYAGEHGIKAIVQPGGSVRDQESIDKANELGIAMVFSGVRHFRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK27</accession>
    <name>H4GK27_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoribosylformylglycinamidine synthase subunit PurS</fullName>
            <shortName evidence="5">FGAM synthase</shortName>
            <ecNumber evidence="5">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Formylglycinamide ribonucleotide amidotransferase subunit III</fullName>
            <shortName evidence="5">FGAR amidotransferase III</shortName>
            <shortName evidence="5">FGAR-AT III</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Phosphoribosylformylglycinamidine synthase subunit III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">purS</name>
        <name evidence="6" type="ORF">PS3_19799</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PurS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000055">
        <property type="protein sequence ID" value="EHS85843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122410.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000055.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19799"/>
    <dbReference type="EnsemblBacteria" id="EHS85843">
        <property type="protein sequence ID" value="EHS85843"/>
        <property type="gene ID" value="PS3_19799"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1259"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01926">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003850">
        <property type="entry name" value="PurS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34696">
        <property type="entry name" value="PTHR34696"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02700">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="SSF82697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00302">
        <property type="entry name" value="TIGR00302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS85843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="81" mass="9014" checksum="16A471DB2B20D49E" modified="2012-04-18" version="1">MTNVRIYVTYKPSVFDPQGETIKNSIHALGHDEVADVTVGKFFDVQINATGDAVEASVKSIAEELLVNFNMETYTYEVREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK51</accession>
    <name>H4GK51_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_14630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000057">
        <property type="protein sequence ID" value="EHS85804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122434.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000057.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS85804">
        <property type="protein sequence ID" value="EHS85804"/>
        <property type="gene ID" value="PS3_14630"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1294"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="242" mass="27108" checksum="6F9C6270B1953934" modified="2012-04-18" version="1">MWNQVKTELQDLTWSSVLLSVLALSFTWLVAPFSYIESLTSHATYTTNFAALILYILCIALSFTIASAHDFVSILSLGPIILSLIVAFIGFFNRPIIMALLMLLPLYLFIIQLPATYLRNTIGLITIGGLFTLSIPVAAVYLVTHRFAWSALIYCLPLALTTWMFLTPFFIRVTRIQSIFITIVGALLLIAILTRPLGIGMILALVAVVISWFWLLSIRKQTPYLAALSTVQLLIMVLIYWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK53</accession>
    <name>H4GK53_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Beta-hydroxyacyl-ACP dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_16624</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioester dehydratase family. FabZ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000058">
        <property type="protein sequence ID" value="EHS85699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122436.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000058.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16624"/>
    <dbReference type="EnsemblBacteria" id="EHS85699">
        <property type="protein sequence ID" value="EHS85699"/>
        <property type="gene ID" value="PS3_16624"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1296"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016790">
        <property type="term" value="F:thiolester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040170">
        <property type="entry name" value="Cytosol_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049">
        <property type="entry name" value="PTHR11049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="8">
        <location>
            <begin position="7"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS85699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="163" mass="18585" checksum="353559FC6C39B3F8" modified="2012-04-18" version="1">MATIKCSDTEVVTTHRILGEDLNEHHTLFGGRLLSIVDAEASIAAARLANRVIVTAAMDHVSFSHPFKLEDVLEMHAYVTGFGKRSIEVFVKVMGEHLLTGERFVGFTCFMTFVVADHEYRVPFDQVIPSTKEQEYLIATYQERQKLRQASREFDHKMSEHIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK62</accession>
    <name>H4GK62_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">DNA ligase</fullName>
            <ecNumber evidence="11 12">6.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Polydeoxyribonucleotide synthase [NAD(+)]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ligA</name>
        <name evidence="14" type="ORF">PS3_15363</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the NAD-dependent DNA ligase family. LigA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.2" evidence="11 12"/>
    <dbReference type="EMBL" id="AICN01000059">
        <property type="protein sequence ID" value="EHS85696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122445.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000059.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15363"/>
    <dbReference type="EnsemblBacteria" id="EHS85696">
        <property type="protein sequence ID" value="EHS85696"/>
        <property type="gene ID" value="PS3_15363"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1307"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003911">
        <property type="term" value="F:DNA ligase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00114">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01588">
        <property type="entry name" value="DNA_ligase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041663">
        <property type="entry name" value="DisA/LigA_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018239">
        <property type="entry name" value="DNA_ligase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033136">
        <property type="entry name" value="DNA_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001679">
        <property type="entry name" value="DNAligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013839">
        <property type="entry name" value="DNAligase_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013840">
        <property type="entry name" value="DNAligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004150">
        <property type="entry name" value="NAD_DNA_ligase_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004149">
        <property type="entry name" value="Znf_DNAligase_C4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01653">
        <property type="entry name" value="DNA_ligase_aden"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03120">
        <property type="entry name" value="DNA_ligase_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03119">
        <property type="entry name" value="DNA_ligase_ZBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12826">
        <property type="entry name" value="HHH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001604">
        <property type="entry name" value="LigA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00532">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="SSF52113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00575">
        <property type="entry name" value="dnlj"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01055">
        <property type="entry name" value="DNA_LIGASE_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01056">
        <property type="entry name" value="DNA_LIGASE_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 11 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 11 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 11 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11 12" id="KW-0520">NAD</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="BRCT" evidence="13">
        <location>
            <begin position="597"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="11">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="42"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="91"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS85696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="679" mass="75058" checksum="3D0E78F41847B3AF" modified="2012-04-18" version="1">MAEVPVSDLSHDQAQAELPTLRAQLIQWGKEYYEQDQPTVEDYVYDRTYQRLVEIESKFPDLVSPDSPTQRVGGQTESQLGKVNHAIPMLSMGDVFSPAELFDFNQRQQASTEVDVETEYNLELKIDGLSLSLVYENGHLVQGSTRGNGQIGEDVTANVKTIKDIPLKLSEPLSMEFRGECYMPKEAFAQLNAEREANGEPVFANPRNAAAGSLRQLDPKITAKRQLAVFMYYIPEYQQLGLQTQAQALEKMAALGFPVNPNYRVVQNDEEIQAYIDEYTVQRDQLPYGIDGIVEKVNDLDTQVALGNTVKVPRWEIAYKFPPEEEPTVVREIEWTVGRTGNVTPTAVMDPVQLAGTTVSRASLHNPDYLMAKDIRIGDTVLLHKAGDIIPEISTVVKAKRPADSQPYQIPTTCPVCGSTLVHLTDEVALRCINPMCPAQIKEGLAHFASRNAMNIDGLGPKIIEQLWDHKLIHDVADLYRLTSEQLLDLPKFGEKSAQNLINSIEQSRHNSVERLIFGLGIRHVGAKAALLLAQRFGSLDELKVAKAQTIAQVDNLGDTIGQSVETYFANPQVQTLLDELKAAGLNFDYLAPQGQVASAEWQDRRVVLTGSLTTMTRQEASNWLSQHGATVTGSVSKKTDLLIAGEKAGSKLTKAQSLGIEIWNEAQFAEAMKGATEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK69</accession>
    <name>H4GK69_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_9962</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000061">
        <property type="protein sequence ID" value="EHS85630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122452.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000061.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9962"/>
    <dbReference type="EnsemblBacteria" id="EHS85630">
        <property type="protein sequence ID" value="EHS85630"/>
        <property type="gene ID" value="PS3_9962"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1318"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="10"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="317" mass="34980" checksum="176407AB67B5C5FA" modified="2012-04-18" version="1">MKAIVIHEPGGPEQLKYEEVPTPVVKPGWSLVKVKAFGINHSEVFTRQGLSPSVNFPRILGIECVGEIVESTDQKLSVGQKVVSLMGEMGRDFDGSYAEYVLLPNHQIYPINSQLSWTDLAALPETYYTAFISLKNMKIDANHQVLVRGATSGVGVAFVNLLKSQFPDIKVSGTTRSVQKTDQLLAAGFDQVVIDDQNHLQTTLSFDRVLELIGPLTIKDTFQHVYEGGIVCITGLLGGQWTLNDFDPIMDLAPNSYLTSGYSGNVSAEKFNELLAFIERYQVKVTPEKIFSLPEVPDAHRYLASQQSFGKVVVKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK71</accession>
    <name>H4GK71_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycine/betaine ABC transporter ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_15787</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000061">
        <property type="protein sequence ID" value="EHS85632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122454.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000061.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15787"/>
    <dbReference type="EnsemblBacteria" id="EHS85632">
        <property type="protein sequence ID" value="EHS85632"/>
        <property type="gene ID" value="PS3_15787"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1320"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="253" mass="28367" checksum="610454BBB546C8AD" modified="2012-04-18" version="1">MTKEVIKFDHVSKSFGNHQVIKDLSFTINAGEFITILGTSGCGKTTTLKMINGLLTASSGQVLVNGLDPQTTDLVTLRRQIGYVVQQVGLFPHMTIADNLSITPKLMGWDQEQIKQRNQELLKLVDMDKGDYLKRYPRELSGGQQQRIGVARALAANPPIVLFDEPLGALDALTRLNLQEDLKKLHQQLNDHTFVLVTHDINEAFFLGQRVMIMDQGHIEQFADPQTIIDQPATPFVKALLQTEIDHQQIWGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK91</accession>
    <name>H4GK91_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Malic enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_14729</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="2">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the malic enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14729"/>
    <dbReference type="EnsemblBacteria" id="EHS85543">
        <property type="protein sequence ID" value="EHS85543"/>
        <property type="gene ID" value="PS3_14729"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1351"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="malic" evidence="5">
        <location>
            <begin position="14"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Malic_M" evidence="4">
        <location>
            <begin position="159"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS85543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="387" mass="41675" checksum="36716113030E53C9" modified="2012-04-18" version="1">MTDEEILSLHARHHGVLNMAPQMAVHNREELGKAYTPGVAVISKLIERYPELKDKYTLSGKLVAMVTDGSAVLGLGNIGPAGGLPIVEGKALLYKYLAGVDAIPLTLDQVPVADFVQTLKNISKSFAGFHLEDIEAPRCFEIEEQLRQAVDIPVYHDDQEGTAIVVLAGLINAAKVVQKPLTDLKVVLNGLGASGVATARLLIKMGIKQLTLFDIDGQVHPDSPEYNQYQREVAQESLVQEHFDNLSSAMVGQDVFIGLSTADVLTATDVRTMAKDPIIFALANPKPEIDPAVAKDGGATIIATGSSQYPNQVNNILAFPGLYKGLLQTDLKQVTWELEAQIAQALAQMISQPEPTKIIPGVFDDGVVATVAQSVIDFAKDQHRKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK94</accession>
    <name>H4GK94_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">L-lactate dehydrogenase</fullName>
            <shortName evidence="6">L-LDH</shortName>
            <ecNumber evidence="4 6">1.1.1.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ldh</name>
        <name evidence="12" type="ORF">PS3_4253</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of lactate to pyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>(S)-lactate + NAD(+) = H(+) + NADH + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:23444"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the LDH/MDH superfamily. LDH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.27" evidence="4 6"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122477.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4253"/>
    <dbReference type="EnsemblBacteria" id="EHS85546">
        <property type="protein sequence ID" value="EHS85546"/>
        <property type="gene ID" value="PS3_4253"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1354"/>
    <dbReference type="UniPathway" id="UPA00554">
        <property type="reaction ID" value="UER00611"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00488">
        <property type="entry name" value="Lactate_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011304">
        <property type="entry name" value="L-lactate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018177">
        <property type="entry name" value="L-lactate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="SSF56327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01771">
        <property type="entry name" value="L-LDH-NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00064">
        <property type="entry name" value="L_LDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ldh_1_N" evidence="10">
        <location>
            <begin position="5"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Ldh_1_C" evidence="11">
        <location>
            <begin position="146"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="121"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="149"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6 7">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="80"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 8">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS85546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="312" mass="33870" checksum="10024604C7206E6E" modified="2012-04-18" version="1">MKNRKVVLVGDGAVGSTFAYSLIQSVKTVEELVIVDLNKERSQGDAMDLQDVTPLTADIDIHTGDYQDAADAAIVVITAGVPRRPGETRIDLVNKNVKILRSIVEPIVESGFKGIFVVSANPVDILTTLTQKISGFPRERVIGTGTFLDKARLNVILGDHFGFKPSEIDALVLGEHGDTSFVNFDEAKVNGRPLKDQVDLTPEVKNQIETEVRQRGGKIIGTKGATFYGVAYGLSTICRAILENNYKMMVISAPLNGQYGIKDLYLGTPAVINSSGIAQVIQTHLSPEEHEKMKRSAEQMQAVIDGVSSMDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKA0</accession>
    <name>H4GKA0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative N-acetylglucosamine-6-phosphate deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_20532</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="7">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the metallo-dependent hydrolases superfamily. NagA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122483.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20532"/>
    <dbReference type="EnsemblBacteria" id="EHS85552">
        <property type="protein sequence ID" value="EHS85552"/>
        <property type="gene ID" value="PS3_20532"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1362"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008448">
        <property type="term" value="F:N-acetylglucosamine-6-phosphate deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00854">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003764">
        <property type="entry name" value="GlcNAc_6-P_deAcase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF14">
        <property type="entry name" value="PTHR11113:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038994">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00221">
        <property type="entry name" value="nagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="8">
        <location>
            <begin position="53"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="217"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="305"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="5">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR038994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS85552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="377" mass="41410" checksum="EAE977437DC12D71" modified="2012-04-18" version="1">MQTVIKNAHIYTGAEEYPNGYLRFNQRVIDVGSMTDFIPSEYDQTVLDASGQVMVPGFIDIHCHGGYGIDTMDADPDQIDRMVHQMTVNEGVTTVFPTTVTQSVDHFDRAVKAVTQAALKNPVIQGIHLEGPFINEKYKGAQPALFIKNPDPTLLRQWQRLANGMVKIITYAPEMPQARKLEKACLEDGVIASAGHTDATYEQMQNSQASHVTHLYNAQRGLHHRQPGVTGYAFLNNQITGEIIADGFHVVPEMVKLAYLVMGAKRLELVTDAMRAKGLPDGESELGGQKVIVKEGQARLESGNLAGSVLLFKQAFKNIITFTGCSINEAVQMASVNQAREFNLPQKGILESGRDADFNLLSAELELAATYSYGQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKA7</accession>
    <name>H4GKA7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mini-ribonuclease 3</fullName>
            <shortName evidence="3">Mini-3</shortName>
            <shortName evidence="3">Mini-RNase 3</shortName>
            <ecNumber evidence="3">3.1.26.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Mini-RNase III</fullName>
            <shortName evidence="3">Mini-III</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mrnC</name>
        <name evidence="5" type="ORF">PS3_7771</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in correct processing of both the 5' and 3' ends of 23S rRNA precursor. Processes 30S rRNA precursor transcript even in absence of ribonuclease 3 (Rnc); Rnc processes 30S rRNA into smaller rRNA precursors.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MrnC RNase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.-" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122490.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7771"/>
    <dbReference type="EnsemblBacteria" id="EHS85559">
        <property type="protein sequence ID" value="EHS85559"/>
        <property type="gene ID" value="PS3_7771"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1374"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01468">
        <property type="entry name" value="RNase_Mini_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008226">
        <property type="entry name" value="Mini3_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005520">
        <property type="entry name" value="UCP005520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="SSF69065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RNase III" evidence="4">
        <location>
            <begin position="13"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="138" mass="15864" checksum="3196C2BAEDEECC7D" modified="2012-04-18" version="1">MATVDQRQLNGIALAYLGDAVYEVFIRQRLLDLGMSKPDRLQKKATHYVSAKAQAALVDLMADEDILTEVEWAYYKRGRNAKSYTHAKNTSILTYRQSTGFEALFGYLKVTEQTERIEELASWCIHQVERGRTEQNDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKC3</accession>
    <name>H4GKC3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">PTS system mannose-specific IIC component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_4102</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122506.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4102"/>
    <dbReference type="EnsemblBacteria" id="EHS85575">
        <property type="protein sequence ID" value="EHS85575"/>
        <property type="gene ID" value="PS3_4102"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1394"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004700">
        <property type="entry name" value="PTS_IIC_man"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03609">
        <property type="entry name" value="EII-Sor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51106">
        <property type="entry name" value="PTS_EIIC_TYPE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="260" mass="27792" checksum="3C33B0CD90046AA2" modified="2012-04-18" version="1">MSIFSLLLVLLISFVAGADGVLDEFQFHQPLVACTLIGMVTGKLIPCVIIGGFLQLITLGWANLGAAISPDIALASVSAEIIYCKLQTVATYRGLTIALAILISIVGSPLTKIIRTQAVGIMHRMEIAAQSGDLVKINRLHCFVMVLQGLRVLVPAVILLILPSRFLMDGARLLPGWLLLGFSIGAGLVVAVSFAVILNMIAAPELWPFFTIGFVLAAIPQLTLLALSLLGLGLVLIFLYLQNGSKTKRYGKSLDDVIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKD0</accession>
    <name>H4GKD0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_16577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122513.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16577"/>
    <dbReference type="EnsemblBacteria" id="EHS85582">
        <property type="protein sequence ID" value="EHS85582"/>
        <property type="gene ID" value="PS3_16577"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1401"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028978">
        <property type="entry name" value="Chorismate_lyase_/UTRA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011663">
        <property type="entry name" value="UTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07702">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00866">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64288">
        <property type="entry name" value="SSF64288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="233" mass="26355" checksum="047FA67DCE3FE254" modified="2012-04-18" version="1">MASPIYIQIHNQLKTMIEQGTWQVGQRIPPERVLAERFQVSRMTLRQAIQTLVDEGVLDRRIGSGTFVASQKVQEKMTGVTSFTELMRAAGRQPSSKTISYHLTAPSETEIQTLRLAPGERVLRMERIRYGDELPICYELATVPENLVSQFSREEITKSFYETLIAKAGLQPGHATQTVSAVNATEKIAEYLGVKRGAALLRMTQISYLQDGRPFEYVHTQYVGNLFEFVLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKE9</accession>
    <name>H4GKE9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_16192</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000064">
        <property type="protein sequence ID" value="EHS85441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122531.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000064.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16192"/>
    <dbReference type="EnsemblBacteria" id="EHS85441">
        <property type="protein sequence ID" value="EHS85441"/>
        <property type="gene ID" value="PS3_16192"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1453"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="3"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS85441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="288" mass="32061" checksum="C5669989840205D7" modified="2012-04-18" version="1">MKLTTTDLTKRYGQKTVVKNLNLEIPAQSLVAFLGNNGAGKSTTISMLIGLLKPTAGQINFLDEHNQPCQPKIGVVFQNSILDNELTVKENLYFRAMMQGKQSVANIGKVTEVLGLSNFLEQKYGSLSGGQKRRVDIARALLNDPDFLFLDEPTTGLDIQTRTVIWQLLNDLRQKNNLTIFLTTHYLEEAHDANLVYIVNQGAVIACGSAAEIIAANTENYLEIRTSHKFAFPANCEKVGQDQYRIIDLPISAAIDFLAQNRAVIEQFEYHHGTLDDAFMNLTGKELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKG3</accession>
    <name>H4GKG3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000067">
        <property type="protein sequence ID" value="EHS85377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS85377">
        <property type="protein sequence ID" value="EHS85377"/>
        <property type="gene ID" value="PS3_13043"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1483"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysR_substrate" evidence="1">
        <location>
            <begin position="36"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="190" mass="21499" checksum="A377A362CB3560E7" modified="2012-04-18" version="1">METWNQVKGQLPNYQLQVVQLPGDLTASNREYSMLGRECDLMIGTFDQATTRSLVQAIPLGTYQFGIAVRSDNPLAMQERVSIEELQDQTLLMVPPGISEKNDQLCQQILATNPNISIEYTIGRYDINVFNQAVDENVALINLTPWKNIHPNLVTLPLETNIEVEYGILAEKHPNNKVKVFMQMIRQLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKG9</accession>
    <name>H4GKG9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AP_endonuc_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_21491</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000067">
        <property type="protein sequence ID" value="EHS85383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21491"/>
    <dbReference type="EnsemblBacteria" id="EHS85383">
        <property type="protein sequence ID" value="EHS85383"/>
        <property type="gene ID" value="PS3_21491"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1495"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="27"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="272" mass="30944" checksum="AC24344C3728B9A5" modified="2012-04-18" version="1">MFMLLGLKASSDWGQIKDRLQYHPDVFEFFTDVPDFTSQGIQHLRQAIEYVQDQGVTNIVLHQPMNFKGYHSELIVPEQQFPELHEFVMTTAHQLIDLAIATDSQCLIHGSYDGEHGFKAMLSAYGSLQNAQAACYHRLDQLKKLGSDHVMFENSISPVFAYGDPQVENEIIRHQFRLVADVSHIFISLHGDNERLVASLRHLKNNIYHYHLVDSLGQTHDSLPIGTGKIDWPSVIPLLNPSATTIYEINLKNQLDCQEQLASHQYLSTLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKI9</accession>
    <name>H4GKI9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_14471</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IMPACT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122571.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14471"/>
    <dbReference type="EnsemblBacteria" id="EHS85269">
        <property type="protein sequence ID" value="EHS85269"/>
        <property type="gene ID" value="PS3_14471"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1533"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015796">
        <property type="entry name" value="Impact_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015269">
        <property type="entry name" value="UPF0029_Impact_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020569">
        <property type="entry name" value="UPF0029_Impact_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="PTHR16301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09186">
        <property type="entry name" value="DUF1949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="UPF0029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00257">
        <property type="entry name" value="IMPACT_YIGZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00910">
        <property type="entry name" value="UPF0029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0029" evidence="2">
        <location>
            <begin position="19"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1949" evidence="3">
        <location>
            <begin position="140"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="212" mass="23745" checksum="C1B6115616BB983A" modified="2012-04-18" version="1">MANIFLTIKTDTSHELVIKKSRFICHLKKIASEEEAREFISAIGQAYRKANHNCFAYLIGDHDEIQRESDNGEPSGTAGVPILEALQKRGLHNVCAVVTRYFGGIKLGAGGLIRAYSNATSTAIEEAGIVQRITQTILSIEVSYKLHDQLLYYLQQIPLEIADESYAVNVTTQVYVDNDQIQTVIDDLTSRFNDQLTLTPGEERQHEVPYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKJ8</accession>
    <name>H4GKJ8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_20023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122580.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20023"/>
    <dbReference type="EnsemblBacteria" id="EHS85278">
        <property type="protein sequence ID" value="EHS85278"/>
        <property type="gene ID" value="PS3_20023"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1543"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="3"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="336" mass="37810" checksum="10DECF130C5E5E72" modified="2012-04-18" version="1">MLKLGIIGTNIITDQMLDAAKTSGRYQLTAVYSRTTKRAKEFGKPYGATQFFDDLESFFDEGDFDVVYIASPNSVHYLQVRLAIENDKFVILEKPAFVNPEEFSRIESLLQAHPKARLVEAARHIHTGLFHESENQLAKMETVQGANFTVMKYSSRYDKVLAGEKPYPNIFTPEFAGGALMDLGVYAVYASVTLFGAPNSVVYFPTMVKSGVDGLGTALLTYDNFSVTLNFGKTANSHLVSEIYGLKDTLVYDSIFDTTKVTYFDANKEAHELSAKYYENSMVDELNDFANLFENPNDEVQAALYKKWFELARTVNMVMFNLRESADLTFPADNNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKK0</accession>
    <name>H4GKK0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytokinin riboside 5'-monophosphate phosphoribohydrolase</fullName>
            <ecNumber evidence="1">3.2.2.n1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_10548</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LOG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.n1" evidence="1"/>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122582.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10548"/>
    <dbReference type="EnsemblBacteria" id="EHS85280">
        <property type="protein sequence ID" value="EHS85280"/>
        <property type="gene ID" value="PS3_10548"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1545"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009691">
        <property type="term" value="P:cytokinin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005269">
        <property type="entry name" value="LOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031100">
        <property type="entry name" value="LOG_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03641">
        <property type="entry name" value="Lysine_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00730">
        <property type="entry name" value="TIGR00730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0203">Cytokinin biosynthesis</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="189" mass="20732" checksum="C8C35899BBBA262E" modified="2012-04-18" version="1">MIKSLAVYCGANPGHNPEFIKLAQRFGQELADHQISLVYGGGQYGMMGAVANSVLDHGGQVHGVITRELYDRGTSLDRLSDLQIVPNMDIRKDKMMNLADGLIALPGGLGTLEEVSEAFSWTAIGDNAKPVALFNFNGYYNPLKSMLGKMQVAGFAERDFVESIGFIATFNELLDFMDNYQAPAVRTYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKK6</accession>
    <name>H4GKK6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable cell division protein WhiA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">whiA</name>
        <name evidence="8" type="ORF">PS3_13027</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in cell division and chromosome segregation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WhiA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000070">
        <property type="protein sequence ID" value="EHS85233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122588.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000070.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13027"/>
    <dbReference type="EnsemblBacteria" id="EHS85233">
        <property type="protein sequence ID" value="EHS85233"/>
        <property type="gene ID" value="PS3_13027"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1560"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043937">
        <property type="term" value="P:regulation of sporulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01420">
        <property type="entry name" value="HTH_type_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018478">
        <property type="entry name" value="Sporu_reg_WhiA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003802">
        <property type="entry name" value="Sporulation_regulator_WhiA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023054">
        <property type="entry name" value="Sporulation_regulator_WhiA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039518">
        <property type="entry name" value="WhiA_LAGLIDADG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37307">
        <property type="entry name" value="PTHR37307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02650">
        <property type="entry name" value="HTH_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14527">
        <property type="entry name" value="LAGLIDADG_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10298">
        <property type="entry name" value="WhiA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00647">
        <property type="entry name" value="DNA_bind_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 4" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WhiA_N" evidence="6">
        <location>
            <begin position="20"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="LAGLIDADG_WhiA" evidence="7">
        <location>
            <begin position="124"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="HTH_WhiA" evidence="5">
        <location>
            <begin position="219"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="310" mass="35353" checksum="853CA61F0AC0ABBB" modified="2012-04-18" version="1">MSYASEVKKELTGIEVRDQNARAELMALIRMNGALGIANHEMTLNIQTESPAIARRIYSLIRQLYQVECDISVRRKMKLKKNNTYVVRLNYHAQDILGDLGILEGFQIKERVPVELLADDLMIRSYLRGAFLAGGSVNNPETSRYHLEIYSLYEEHNEMIAEMINQYSLNARMTNRRSGYIVYLKEAEKIADFLQLIGATTSMLKFENIRIVRDMRNSVNRLVNCENANLDKIANASNKQIENIKYIDARVGLGNLPEKLRAIAETRLQHQEVSLKELGELVPGGPISKSGVNHRLRKINDYADQLRAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKQ0</accession>
    <name>H4GKQ0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleic-acid-binding protein implicated in transcription termination</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_5337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122632.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5337"/>
    <dbReference type="EnsemblBacteria" id="EHS84970">
        <property type="protein sequence ID" value="EHS84970"/>
        <property type="gene ID" value="PS3_5337"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1621"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00279">
        <property type="entry name" value="YlxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007393">
        <property type="entry name" value="DUF448"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037465">
        <property type="entry name" value="YlxR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035931">
        <property type="entry name" value="YlxR-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34215">
        <property type="entry name" value="PTHR34215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04296">
        <property type="entry name" value="YlxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64376">
        <property type="entry name" value="SSF64376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YlxR" evidence="1">
        <location>
            <begin position="9"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="99" mass="11524" checksum="440C9D6C14BAF16B" modified="2012-04-18" version="1">MKQRKIPMRKDVVTGEMVPKRQLIRVVRNKENEVSLDPTGKKPGRGAYVSIDVKVAQKAKQKRTFDKVFDVQLSDEFYDELIAYTDHQQARQELFGQND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKR6</accession>
    <name>H4GKR6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6 7">Elongation factor Ts</fullName>
            <shortName evidence="6">EF-Ts</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tsf</name>
        <name evidence="10" type="ORF">PS3_7728</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6 7">Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the EF-Ts family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122648.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7728"/>
    <dbReference type="EnsemblBacteria" id="EHS84986">
        <property type="protein sequence ID" value="EHS84986"/>
        <property type="gene ID" value="PS3_7728"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1639"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00050">
        <property type="entry name" value="EF_Ts"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036402">
        <property type="entry name" value="EF-Ts_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001816">
        <property type="entry name" value="Transl_elong_EFTs/EF1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014039">
        <property type="entry name" value="Transl_elong_EFTs/EF1B_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018101">
        <property type="entry name" value="Transl_elong_Ts_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11741">
        <property type="entry name" value="PTHR11741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00889">
        <property type="entry name" value="EF_TS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="SSF46934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54713">
        <property type="entry name" value="SSF54713"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00116">
        <property type="entry name" value="tsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01126">
        <property type="entry name" value="EF_TS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01127">
        <property type="entry name" value="EF_TS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="4 6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF_TS" evidence="9">
        <location>
            <begin position="71"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Involved in Mg(2+) ion dislocation from EF-Tu" evidence="6">
        <location>
            <begin position="80"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS84986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="292" mass="31664" checksum="734EF827E71D778B" modified="2012-04-18" version="1">MAEISAKLVMELRKKSGAGIMDAKKALVASDGDMDKAMDYLREKGIAKAAKKSDRIAAEGLTNIVVNGNTAAVVELNSETDFVAASDPFKEELAEVSSLIAENKPADVEAALQIKTANGTVNDDLIATTQKTGEKVSLRRFEIVEKNDGDNFGAYLHQGGRIAALVVLEGADEETAKDVAMHVAAINPEFMTREDVSAERLAHEKEVFTEETLNEGKPANIVDKIVEGRLNKFLSQICLADQDFVKDSDQTVAQFVASKGGKLKSFIRYEVGEGIEKKETNLAQEISEQLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKS7</accession>
    <name>H4GKS7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Na-H antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_9411</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122659.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9411"/>
    <dbReference type="EnsemblBacteria" id="EHS84997">
        <property type="protein sequence ID" value="EHS84997"/>
        <property type="gene ID" value="PS3_9411"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1655"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="221"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="359"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="5">
        <location>
            <begin position="525"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS84997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="608" mass="67759" checksum="F67DD4158A8AEFF2" modified="2012-04-18" version="1">MQLSIFIVMAVALAVPILMARFNVTAVPTAIAEIVIGMLIGQSGFGWVQTNSSLTLLSSLGVIILMFLSGMEIDFSLFKRAEKPKSGANPVKMATGAFLMNVVMALIIATILKFTGLFTDLFLSLIIFTTVALGVVIATLKEKEILSRPVGQTILLTAVFGEIFPMFMLTVYGSINGGNARGIWLIVLPLIVAIILLNRFKQPYLWFAKMTKATTQLDIRLAFFLIFTLVIVAERVGAENILGAFLAGMVMKLLEPSEATMDKLTSIGYGFFIPIFFIMTGVKLDLRSLFANPNALALIPVMVASFILAKIFSFLVFRRQFSNRNSFAAAFLTVTTITLVLPSLQVARNLKMISATQSDAVTLAAIVVCIISPIIFNSTFKLEKADKIKQRVVFIGTNILTVPIAQQLSSNWYDVRMVTNKLENYQVYDSEFDNLTLIEEISADKLLDQGFLDADVIVYGFRDDKLNAKLSLATKEHSNARVITSFQNREYLDQVFDKLAAAGIEVFNLYQVQSTFLREWIESPSLIQMLQDNEAGIFEVEIRDHRYSGQQLMALPFIDKMTISRIRRGKEWITPHGTTVIEYGDRLIFTAKHHRDVVAIRQALSQPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKT8</accession>
    <name>H4GKT8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_12656</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122670.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12656"/>
    <dbReference type="EnsemblBacteria" id="EHS85008">
        <property type="protein sequence ID" value="EHS85008"/>
        <property type="gene ID" value="PS3_12656"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1666"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="SSF47005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3 4" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="8" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="6">
        <location>
            <begin position="4"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="7">
        <location>
            <begin position="133"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="84"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="436" mass="46215" checksum="B5F05F658A6AB52E" modified="2012-04-18" version="1">MSSKYQFKLPDVGEGMAEGTVGEWHVAVGDQVEKDQDFVQIENDKSVEELPSPVAGTVTKIIVPEGELANVGDPLIELEVADGEGNVDESAEEAAPAEEAAPAAAEPSAPAPAPAASPAAPAPVAADHSLPVLAMPAVRKFARDNGVDVNQVAGTGRHGQVLKSDVEAFLAGGAPVAAPAAEVSAPTPAAEPAPMAVPTAPEGWPEHAEKMSPVRKATAKAMTTASHEIPMITVFDEVVVDKLWDHRKKYKELAAERGVHLTFMAYMTKALAVIMREFPEFNAKVDMENKQIHYRDYVNVGIATDTDNGLYVPNVKHADRLSLFDIAKAISANTEKAKDGKLGSADMSNTGMTITNIGSIGGGHFTPIVNWPEVAIIGMGKISSEPVVVEDHVEVARVLKLSLTVDHRIIDGATAQRAMNRMKELLADPELLLMEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKU1</accession>
    <name>H4GKU1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Branched-chain amino acid transporter azlD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_14616</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122673.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14616"/>
    <dbReference type="EnsemblBacteria" id="EHS85011">
        <property type="protein sequence ID" value="EHS85011"/>
        <property type="gene ID" value="PS3_14616"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1669"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008407">
        <property type="entry name" value="Brnchd-chn_aa_trnsp_AzlD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05437">
        <property type="entry name" value="AzlD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003203">
        <property type="entry name" value="AzlD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="110" mass="12187" checksum="52239898003CA1F7" modified="2012-04-18" version="1">MTLIQQLATILIAAVANMTTRIVPFKLFRDQNGHVSPYIDGLGKFLPPAIMAMLVVYCYRSVNWLGASHGLPDLIAGIVTVIVHLIWRSLFTSLILGTVAYIVLINFVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKW8</accession>
    <name>H4GKW8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Penicillin binding protein 1A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PS3_11351</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11351"/>
    <dbReference type="EnsemblBacteria" id="EHS85038">
        <property type="protein sequence ID" value="EHS85038"/>
        <property type="gene ID" value="PS3_11351"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1704"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Transgly" evidence="10">
        <location>
            <begin position="62"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="9">
        <location>
            <begin position="339"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="662"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="265"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS85038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="723" mass="78065" checksum="76B5FBA48CF017CA" modified="2012-04-18" version="1">MGYFLVDHKALWKRIFLWLVGIFVLLVIAGAALFFYYASSAPTITKSDLQSQTGTTIYDANGKVVSRLGAQKRQYASSSELPKTLKNAVVAVEDKNFYHEKLGISPTRIVKAAFSNVFHGTSDGIQGGSTITQQLIKLSVFSTAKSDQTFKRKAQEAWLAYNITKKFSKDQILTYYLNKVYMGNGVYGMKTAAQYYYGKDLDELSLAQTAMLAGIPQSPSVYNPLANTTYAKERRDVVLDAMQTNGYITASQAAAAKKESVTKGLDSSHGNVESSSSQVDEPVVDAYVKQVIEELEDKGYNPTTDSLKVHTSLDTDAQQELYDDANENVSFPSDNVQVGVAVVDPHNGQVIAMLGGRKLDSNTVYGYNRAVQQTRSSGSTAKPLMDYGPAIEYLHWPTFHVLSDTAMTWPDATGTIKDFDNKYQGNITMRQALVQSRNVPAIRALQAVGISKATKFLKGLGISQKKAYTYSSGIALYISPLQEAAAYAAFANGGTYYEPYYVTSVTTQEGTTTNFKKQGSRAMTKGTAFMITNMLEGVFTGNGSGTSAYLSGVNQAGKTGTTNASDNNNNSGILDSWMTGYTKNYSISVWTGYDDNKNTLTDSGAYSAQYLYKALMSYLDGENHASNWTMPSSLESVSVQGVTQYMFKAYPFSIEYASGSSTSSTSSSSSTSSSSSSSDSSSASSESTSSASSSMLSESVQTITQNSQSTTDANTNNAASSGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKX4</accession>
    <name>H4GKX4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptide chain release factor 3</fullName>
            <shortName evidence="7">RF-3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">prfC</name>
        <name evidence="9" type="ORF">PS3_15047</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. PrfC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000073">
        <property type="protein sequence ID" value="EHS84948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122706.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000073.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15047"/>
    <dbReference type="EnsemblBacteria" id="EHS84948">
        <property type="protein sequence ID" value="EHS84948"/>
        <property type="gene ID" value="PS3_15047"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1711"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04169">
        <property type="entry name" value="RF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00072">
        <property type="entry name" value="Rel_fac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004548">
        <property type="entry name" value="PrfC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032090">
        <property type="entry name" value="RF3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038467">
        <property type="entry name" value="RF3_dom_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041732">
        <property type="entry name" value="RF3_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43556">
        <property type="entry name" value="PTHR43556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16658">
        <property type="entry name" value="RF3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00503">
        <property type="entry name" value="prfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="12"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="21"/>
            <end position="28"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="89"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="143"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS84948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="526" mass="59118" checksum="E14848EC7A9D02A4" modified="2012-04-18" version="1">MTGKQNLANAVNQRQTFAIISHPDAGKTTITEQLLLFGGVVREAGTVKGRGSNNFAKSDWMEIEQKRGISVTSSVMQFDYAGKRINILDTPGHEDFSEDTYRTLMAVDSAVMVIDGAKGIEPQTRKLFQICKMRGIPIFTFINKYDRDAREPLDLMNEIEETLGIETYPMDWPIGMGRQFMGVYDRHNHRVALTHPQDEANPYLPLDDQGEVIGENPLAGAGEWTDALESMELINEAGSEFSAAKIAAGDQTPVFFGSALTNFGVKTFLETYLQYAPAPSAHKTESGVAIDPLSTDFSGFVFKIQANMNPKHRDRIAFVRICSGEFDRGMDVTLERTQKPLRLSNVTEFMANTRENVETAVAGDIIGLYDTGNFQIGDSIYTGKTPIKFEKLPQFTPELFVRVSAKNVMKQKSFHKGINQLVQEGAVQLYTSYTTNDYILGAVGQLQFEVFKFRMQNEYNSEVIMEPMGNKTARWINPDQLDPKMSSSRNILVKDINGAPLFLFENQFAENWFKQKYPDVELEKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKX8</accession>
    <name>H4GKX8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Elongation factor G</fullName>
            <shortName evidence="7">EF-G</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fusA</name>
        <name evidence="9" type="ORF">PS3_6148</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000076">
        <property type="protein sequence ID" value="EHS84830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122710.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000076.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6148"/>
    <dbReference type="EnsemblBacteria" id="EHS84830">
        <property type="protein sequence ID" value="EHS84830"/>
        <property type="gene ID" value="PS3_6148"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1719"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01434">
        <property type="entry name" value="EFG_mtEFG1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03713">
        <property type="entry name" value="EFG_mtEFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_B">
        <property type="entry name" value="EF_G_EF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004540">
        <property type="entry name" value="Transl_elong_EFG/EF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00484">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7 9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="10"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="83"/>
            <end position="87"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="137"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS84830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="695" mass="76466" checksum="457FCE7157AF46CC" modified="2012-04-18" version="1">MANKREYPLEKTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETHDGASQMDWMEEEQERGITITSAATTAVWKNHRINIIDTPGHVDFTVEVERSLRVLDGAVTVLDAQAGVEPQTETVWRQADDFNVPRIVFANKMDKMGANFDYTVSTIKDRLNVTPLPIQLPIGAEDDFIGLVDLVTMEAYLYDNDDQGATWDTVEIPADLKDKAEQQHEELIETLADVNDDIMAKYLEGEEISVEEIKAAIRQATLDQELFPVLAGSAYKDKGIQMMLDAVLDYLPSPLDVKPFVATDEDGNEIELTAGDDKPFAALAFKIATDPFVGRLTFLRVYTGSLQSGSYVLNATKGKRERVGRLLQMHSNQQNEIPEVFSGDIAAAIGLKNTTTGDSLTDPDKPLQLESMEFPEPVIQVSVEPKSKADQDKMDKGLQKLAEEDPTFKAETNPETGETLIAGMGELHLDIIVERLRREFNAEVTVGKPQVSYREAFTNTVEAQGKFVRQSGGKGQYGDVWIEFTPLEEGAGFEFEDAIVGGVVPREYIPAVEQGLKEAMENGVLAGYPLVDLKAKLYDGSYHEVDSSEAAFKVAASLALRNAAPKGGATILEPIMKVDIVTPEDNLGDVMGHVTARRGSIDGMEERGNAQLVHAFVPLSEMFGYATTLRSATQGRGTFSMTFDHYSAVPKSIQEEIIEKNGGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKY0</accession>
    <name>H4GKY0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4 6">30S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpsL</name>
        <name evidence="8" type="ORF">PS3_9724</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 6">Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1 4 6">With S4 and S5 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 6">Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4 5">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000076">
        <property type="protein sequence ID" value="EHS84832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122712.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000076.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9724"/>
    <dbReference type="EnsemblBacteria" id="EHS84832">
        <property type="protein sequence ID" value="EHS84832"/>
        <property type="gene ID" value="PS3_9724"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1721"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03368">
        <property type="entry name" value="Ribosomal_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00403_B">
        <property type="entry name" value="Ribosomal_S12_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_S12/S23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005679">
        <property type="entry name" value="Ribosomal_S12_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="PTHR11652"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01034">
        <property type="entry name" value="RIBOSOMALS12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00981">
        <property type="entry name" value="rpsL_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 5 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="137" mass="15233" checksum="480F81CF3274E8DC" modified="2012-04-18" version="1">MPTINQLVRKGRKSHKGKSKSPALGYVYNSFKKEEVKTPSPQKRGVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKDLPGVRYHIIRGTLDTAGVEGRMQSRSKYGAKKPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKY5</accession>
    <name>H4GKY5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_16211</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000076">
        <property type="protein sequence ID" value="EHS84837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122717.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000076.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS84837">
        <property type="protein sequence ID" value="EHS84837"/>
        <property type="gene ID" value="PS3_16211"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1727"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="8"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS84837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="196" mass="22942" checksum="251B1469C8E17EF9" modified="2012-04-18" version="1">MKREEKLERTHKAILKTATKLFLNRGYAQTSTRDIAKQIGITQPALYHHFKDKEVLYLDVMNEHGTRVRQEIQRVLRNKNLDIKQQLQEITNVYLKLHPQSVYSYYQEARRQLSLSSRRKLDTIFTMDYINPLAQYFAQDIVNLRPDVRPREAAELYLAELQPLHGTVLSLGGHAVSPEQRQRVLLDCILNGFIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKZ8</accession>
    <name>H4GKZ8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Adenylosuccinate synthetase</fullName>
            <shortName evidence="8">AMPSase</shortName>
            <shortName evidence="8">AdSS</shortName>
            <ecNumber evidence="8 10">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purA</name>
        <name evidence="11" type="ORF">PS3_5349</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 10">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="8 10"/>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122730.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5349"/>
    <dbReference type="EnsemblBacteria" id="EHS84764">
        <property type="protein sequence ID" value="EHS84764"/>
        <property type="gene ID" value="PS3_5349"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1741"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033128">
        <property type="entry name" value="Adenylosuccin_syn_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="PTHR11846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00184">
        <property type="entry name" value="purA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00513">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 10" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="IMP binding" evidence="8">
        <location>
            <begin position="13"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="8">
        <location>
            <begin position="38"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="298"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="12"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="40"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="330"/>
            <end position="332"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="412"/>
            <end position="414"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS84764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="429" mass="47330" checksum="6FBCE0BE7401E0F3" modified="2012-04-18" version="1">MASVVIVGSQWGDEGKGKMTDYLSQDADVVVRSQGGNNAGHTIAFDDKKFSLRLVPSGIFSPGKLAVVGNGVVVNPKALLEELHYLQEKGIDVSGLRISNRAHLTMPYHILLDKCQEAAKGDHKVGTTQNGIGPTYMDKVSRIGIRMCDLLEKDTFAEKLKQNLAIKNDLFTKVYNVDPINFDDIFEEYYQYGQELKQYVTDTSVLISDALTNGQKVLFEGAQGTMLDIDHGTYPYVTSSVPTAGGAATGAGVGPELINTVVGICKVYSTRVGAGPFPTELHDEIGDHIRETGHEYGTVTGRARRVGWFDSVAMRHAQRVNGMNYLSLNLLDVLDGLDTVKICRSYKLDGEIINYYPASLAELERCEPVYEEWPGWQEDITGVTEFDQLPENAQKYLRRLEELSNAKLATISVGPDREQTIILTNPWEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL00</accession>
    <name>H4GL00_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UDP-N-acetylglucosamine 2-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_13738</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-N-acetylglucosamine 2-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122732.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13738"/>
    <dbReference type="EnsemblBacteria" id="EHS84766">
        <property type="protein sequence ID" value="EHS84766"/>
        <property type="gene ID" value="PS3_13738"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1746"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008761">
        <property type="term" value="F:UDP-N-acetylglucosamine 2-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003331">
        <property type="entry name" value="UDP_GlcNAc_Epimerase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029767">
        <property type="entry name" value="WecB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43174">
        <property type="entry name" value="PTHR43174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02350">
        <property type="entry name" value="Epimerase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00236">
        <property type="entry name" value="wecB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase_2" evidence="2">
        <location>
            <begin position="23"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS84766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="371" mass="41472" checksum="B305CC64BBAE78A5" modified="2012-04-18" version="1">MQRTIMTVFGTRPEAIKMMPVVKALEASSDFRSVVVVTAQHREMLDQVLEDFDLHPDYDFNLMKSGQTLTDITNRVLSEMAKVIPEIQPEMVLVHGDTSTTMAAALAAFYQQIPVGHVEAGLRMWQKYDPFPEEMNRQITDDLATLYFAPTDQSKQNLLLEHHPEKQIVVTGNTAIDALQYTVTQDFKHPVLAQIKNPNAKLLLVTMHRRENQGEPMRRALTAIKEVVESQPDVEVIFPAHLSPRVQKVAHEVFDGVERVHIIDPLEVIDFHNLAARSYLILTDSGGVQEEAPSLGKPVLVLRDTTERPEGVTAGTLRLVGTDPQVIKTQLTALLTNKARYQQMATAPNPYGDGHAAERILESLTEYLGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL62</accession>
    <name>H4GL62_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tetratricopeptide TPR_2 repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_7787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122794.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7787"/>
    <dbReference type="EnsemblBacteria" id="EHS84692">
        <property type="protein sequence ID" value="EHS84692"/>
        <property type="gene ID" value="PS3_7787"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1821"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="28"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="101"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="135"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="223" mass="25777" checksum="4A37DE954E2D154D" modified="2012-04-18" version="1">MDPKVQSNQSEKLVHSLVQAIDQEPTKFNNYYELGSLLTRLQDFEQAEELFMKALGIFEQRDDSRAVDFLKYGLGNLYYTVDKVDEAIKYYDQVSDENLKADAYLMMAQSYVKKHDYKRAIVYGLTAYEARPSDPEIAQVLGDALLATGEFKEAAKYYDQILTRHPGRADTQFNRGLVAMAMNEPYTEYLEQAQQLDPDYYKASEQRIEEIKATLQQLNLDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL63</accession>
    <name>H4GL63_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglycerate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_18360</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122795.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18360"/>
    <dbReference type="EnsemblBacteria" id="EHS84693">
        <property type="protein sequence ID" value="EHS84693"/>
        <property type="gene ID" value="PS3_18360"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1822"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS84693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="219" mass="24928" checksum="C5234E7DD7598C0B" modified="2012-04-18" version="1">MTTFYFIRHGKTEWNLQGRYQGANGNSPLLPESHHDIKLLAQYLRGTEFKHAYTSPLLRAQETATELIDQLGMPIPLTLDKRIAEVNLGDLEGMYYRDAQKQWPTVIDNFHYHADSYNEQIIHGESFPHVIERFKEAIRDYEHFNPTGNVLVVSHGAALNAGINGILGVPLAHLKDRGGLSNTSTTIVTTEDQGMTFELQQWNETSYLQRQFIDPTDTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL78</accession>
    <name>H4GL78_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Penicillin binding protein 2B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_8096</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transpeptidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8096"/>
    <dbReference type="EnsemblBacteria" id="EHS84708">
        <property type="protein sequence ID" value="EHS84708"/>
        <property type="gene ID" value="PS3_8096"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1841"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005543">
        <property type="entry name" value="PASTA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005311">
        <property type="entry name" value="PBP_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036138">
        <property type="entry name" value="PBP_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03793">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03717">
        <property type="entry name" value="PBP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00740">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56519">
        <property type="entry name" value="SSF56519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51178">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="PASTA" evidence="6">
        <location>
            <begin position="597"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="PASTA" evidence="6">
        <location>
            <begin position="657"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS84708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="715" mass="77586" checksum="3EBB2CEB52522383" modified="2012-04-18" version="1">MPKIRIKANKSNQRDPKAFGKWLFFVVGALFVVFGIRFAYISIGKHVKGYDLATLAKQRYTSSSTIQAKRGKIYDANGGTLATNTSRYTVYAIISHSYKSTSGKKLYVQNKRKTAKILAKQLGMSEKKVYQLLNPANKKTYQVQFGTKGSNLTASQMLKIKQYHLPGIKFTKTSAREYPEGNFLSQILGQTTTKVNSKTHQTSLVGTMGLESYFNKILSGENGVKSVRQDVYGYKLSSSEAGGRKVKDGGNVYTTIDEQVQNMLETTMQSVEDKTQAESLTGIVVEAKTGKILAASQRPNLTSDTPTWVNALTQTTYEPGSTMKIFALSAAINSGKFNPNKTYQSGSYSLGGGKITDWKTEGWGTITYQEAIDRSSNVGFAHIEQDMGAKTWLKYIKAFGFLKAAKVYGLGQEGSGSTTYSGSLEQANTAFGQGITVTGMQIIQALTAVANNGKMMQPYIISKVTDSNGKTIKKIKPKQIATPITSKTSKQVLNYLKGVIYNDDGTGQAYKVDGYTIAGKTGTAQIAGSNGSYETGTYDYIYSFAGFAPANNPRYIVYVTMTKPKTVDQSAENTISTITTPIIKFLLDRDKSASKKSPGTIKMPNLIGMNINSAAKSLTKNNISVTTLGSGKKVTKQSVTAGERVMVKNRIFLLTGGTYQMPDVSGWSQADVNELGQLLGLKVKTSGTGFVSKQSITQGTTIKQGQTLTVTLAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL83</accession>
    <name>H4GL83_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">rRNA methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_11886</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122815.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11886"/>
    <dbReference type="EnsemblBacteria" id="EHS84713">
        <property type="protein sequence ID" value="EHS84713"/>
        <property type="gene ID" value="PS3_11886"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1849"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016718">
        <property type="entry name" value="rRNA_m1G-MeTrfase_A_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018249">
        <property type="entry name" value="MyrA_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Methyltranfer_dom" evidence="3">
        <location>
            <begin position="94"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="2">
        <location>
            <begin position="104"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR018249-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR018249-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS84713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="284" mass="32740" checksum="AF4CFA030764FE53" modified="2012-04-18" version="1">MKKIKVVETWLSEHLAMFQCPVCQSAIVNLDQHSLICSNRHFTNINKHGYVYFMNHAAKGEYDREMLLDRRKLLQAGLFKPMVEVVARRLSAQDETILDVGTGEGTPLDQIRQARANYNDTYLGFDIAKDGVQLATQLDPHLFFCVADLRKLPFQSQQFSTIVEFFSPSDYGEFKRVLKPGGQVLKVIPNPDYLIELRQLLYADDQDHQTYSNQSVKDLFKVHYPQMEEERIQYQFDLPFDLRSSLVKMSPLHWGKAAKTLTDEDLIKLTQVTVDVQLLIGHQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL98</accession>
    <name>H4GL98_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Holliday junction ATP-dependent DNA helicase RuvA</fullName>
            <ecNumber evidence="8">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ruvA</name>
        <name evidence="10" type="ORF">PS3_5386</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Forms a complex with RuvB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RuvA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122830.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5386"/>
    <dbReference type="EnsemblBacteria" id="EHS84597">
        <property type="protein sequence ID" value="EHS84597"/>
        <property type="gene ID" value="PS3_5386"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1874"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009379">
        <property type="term" value="C:Holliday junction helicase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14332">
        <property type="entry name" value="UBA_RuvA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00031">
        <property type="entry name" value="DNA_helic_RuvA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013849">
        <property type="entry name" value="DNA_helicase_Holl-junc_RuvA_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000085">
        <property type="entry name" value="RuvA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011114">
        <property type="entry name" value="RuvA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036267">
        <property type="entry name" value="RuvA_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07499">
        <property type="entry name" value="RuvA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01330">
        <property type="entry name" value="RuvA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46929">
        <property type="entry name" value="SSF46929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00084">
        <property type="entry name" value="ruvA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 8 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="HhH1" evidence="9">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="9">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS84597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="196" mass="21412" checksum="75BE4B2297A1D646" modified="2012-04-18" version="1">MFNYLNGLITEVTPDYIVVDIQGVGYQVQVANPYAYQAGVKDPVKVYIYQSVREDSITLFGFQSSADKHLFLQLISVSGIGPKSALAILETPDHQALIEAIQQGDDKYLAKFPGIGKKTAGRIIVELRDRLTIDEQPMNATASMPISDDPALMDAMAALIALGYTEKQVAKVEKTLQKEAAGSTNDYISRGLKLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLA3</accession>
    <name>H4GLA3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Competence/damage-inducible protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_14858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14858"/>
    <dbReference type="EnsemblBacteria" id="EHS84602">
        <property type="protein sequence ID" value="EHS84602"/>
        <property type="gene ID" value="PS3_14858"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1879"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036653">
        <property type="entry name" value="CinA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008136">
        <property type="entry name" value="CinA_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02464">
        <property type="entry name" value="CinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142433">
        <property type="entry name" value="SSF142433"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00199">
        <property type="entry name" value="PncC_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CinA" evidence="1">
        <location>
            <begin position="8"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="163" mass="16999" checksum="E7C05A0E0DC3FAF2" modified="2012-04-18" version="1">MLNQQLVNVAQEVVAALKDRQWKITAAESLTAGMFQSTIASVAGASAIFDGGFVTYAASAKANLLKIDPQLIQQHGVVSSPVAGAMARNASQIMKAQIGVGLSGVAGPDSLEGHPAGTVWLGIAINGQVKTKCLHLDASQGRQAVRQQSVLEALRIVLNELEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLB7</accession>
    <name>H4GLB7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cell division regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PS3_19760</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19760"/>
    <dbReference type="EnsemblBacteria" id="EHS84616">
        <property type="protein sequence ID" value="EHS84616"/>
        <property type="gene ID" value="PS3_19760"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1895"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010379">
        <property type="entry name" value="EzrA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06160">
        <property type="entry name" value="EzrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="6 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0717">Septation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="88"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="300"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="438"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS84616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="549" mass="63314" checksum="1531A2D4577ED606" modified="2012-04-18" version="1">MYLYQRNLLKKVNALRDQINVIENYDLEARLSDENVTDFSGETLKKFTELRTKYDEKAMPAVAEAVEIADEIEEDFHGTAALSMGGRVKELQDAVDDAKELLDSIQLEVKAFDDEIEAQNQSSDQLRTWFRKYHKELDEQAYKYGDSVTKLNERLTDLEARFNKFCEIADDGDREAAAEVLADVKNGTVDFEELVEQIPELCQPLLQEFPDQLAKLKQGYGHLISENYHFTEPDIDHLVEALEVKRQATLERVADLDLTQVKETNEGMDSEIDRLYDVMEQEIDARPKVQQMLIPFAEHLTHAKNQNRLLTNELDRLSISYTLNNDEIATVRQLDEQLKQIEADFNADQKRLEAKTAIDSQILANLQAGEKAMTAVEEQQTEINDSVAELQNDEKLARDAVQRFVADLRATKRHVESLNLPGIPMDYMDFFFLVSDELSRLSKDMNKQQINMDEITKKMLVLQDDFEKLHEKTDSIRDSATLTERVIQYAQRLSMQHPELDDAINRSQQLFSEFEYSRALETIGAAVEDIEPGVFKQIEDSYYREVGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHT7</accession>
    <name>H4GHT7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCA-adding enzyme</fullName>
            <ecNumber evidence="4">2.7.7.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">CCA tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA CCA-pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA adenylyl-/cytidylyl- transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA-NT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cca</name>
        <name evidence="8" type="ORF">PS3_81</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">A single active site specifically recognizes both ATP and CTP and is responsible for their addition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121621.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_81"/>
    <dbReference type="EnsemblBacteria" id="EHS87493">
        <property type="protein sequence ID" value="EHS87493"/>
        <property type="gene ID" value="PS3_81"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.56"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052929">
        <property type="term" value="F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052928">
        <property type="term" value="F:CTP:3'-cytidine-tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052927">
        <property type="term" value="F:CTP:tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016437">
        <property type="term" value="F:tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042245">
        <property type="term" value="P:RNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01263">
        <property type="entry name" value="CCA_bact_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032810">
        <property type="entry name" value="CCA-adding_enz_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023068">
        <property type="entry name" value="CCA-adding_enz_firmicutes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13735">
        <property type="entry name" value="tRNA_NucTran2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0692">RNA repair</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="PolyA_pol" evidence="5">
        <location>
            <begin position="27"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="PolyA_pol_RNAbd" evidence="6">
        <location>
            <begin position="174"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA_NucTran2_2" evidence="7">
        <location>
            <begin position="255"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="401" mass="45059" checksum="7D24B8F5D79C279D" modified="2012-04-18" version="1">MIIEQLPTEFKEAEPVLAQIEAAGFEAYFVGGCVRDTILGQPLHDIDIATSAYPSEIKAIFSRTVDTGIEHGTVMILDHGKGYETTTFRTETGYQDFRRPDEVTFVRSLAEDLQRRDFTINALALKRTGEVIDLFDGLHDLTNRLIRAVGNPDERFHEDALRMMRAVRFASQLDFEIDPETIQGITDNAHLLSKIAVERIQVELEKLFVGTNPTKGLQVMMETDLYEYTPTLADQSMTLKNLLALDNWHLTFPVSVWATLMFAGGHDDQTIPGLLKQWKLSNDCIKESRLVVQFLDALQNHQVTPELLFKMNAKRLQWAHEVAQVLNIEHDQATIMRDYAQLPIHDAHELAIKGGDLITGVGIKPGPQLGQIIRQLQVAVLTQKAKNQPVDLIALASKLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHU1</accession>
    <name>H4GHU1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">30S ribosomal protein S1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_8978</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121625.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8978"/>
    <dbReference type="EnsemblBacteria" id="EHS87497">
        <property type="protein sequence ID" value="EHS87497"/>
        <property type="gene ID" value="PS3_8978"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.60"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035104">
        <property type="entry name" value="Ribosomal_protein_S1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10724:SF7">
        <property type="entry name" value="PTHR10724:SF7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00681">
        <property type="entry name" value="RIBOSOMALS1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="23"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="115"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="201"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="286"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="409" mass="44192" checksum="0D0423B7E214C58F" modified="2012-04-18" version="1">MAENENNNAMLEALDSIKPVNVGDVVKGKVLQIDDDRQAVVGIEGSGVEGIVPANQLSTKPVNDINEVVKVGDVLDLVVISKIGSDKENGSYLLSHRRLEAMKVWDEIQAKFDNGETLTATVTQAVRGGLVVDAGVRGFVPASMITDHYVDDLNQFKGQELEFKIVEIEPSENRLILSHKEIVQEQHAEAAAKIFGELAPGDVVEGKVARMTNFGAFIDLGGVDGLVHVSEISYDHVDKPADVLEVGEDVKVKVLSVDPERERISLSIKQTLPGPWDDIESKAPQGTVLTGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPADVLKVGEEVQVKVLNVEADKQRLGLSIKALQEAPKDAESNNGGARRPRRRNNNNNFAADAPEEETGFTLGDLIGDELKNAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHU5</accession>
    <name>H4GHU5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Riboflavin transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_4084</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Probably a riboflavin-binding protein that interacts with the energy-coupling factor (ECF) ABC-transporter complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the prokaryotic riboflavin transporter (P-RFT) (TC 2.A.87) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121629.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4084"/>
    <dbReference type="EnsemblBacteria" id="EHS87501">
        <property type="protein sequence ID" value="EHS87501"/>
        <property type="gene ID" value="PS3_4084"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.65"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032217">
        <property type="term" value="F:riboflavin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024529">
        <property type="entry name" value="ECF_trnsprt_substrate-spec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025720">
        <property type="entry name" value="RibU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38438">
        <property type="entry name" value="PTHR38438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12822">
        <property type="entry name" value="ECF_trnsprt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037778">
        <property type="entry name" value="UCP037778_transp_RibU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="154"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="190" mass="21165" checksum="8CFF931876BC0C53" modified="2012-04-18" version="1">MTESHRRIQQITFIAVLSCLAFILMIFELPNPFSVASYLKLDFSDIPVLLATWMYGPLSGFMVAFIKCFLHASMNGFSDWLGVLSSLASSLCILLPFAWVFKKVKWKFAKQLYIGGAVSTVSLTVVMSALNWLVLVPVYMSLWGWHPSVPVPQLVALGVVPFNILKGILIAVIFAIIAKPLQSVLKHYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHW7</accession>
    <name>H4GHW7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8 10">FAD:protein FMN transferase</fullName>
            <ecNumber evidence="2 8 10">2.7.1.180</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Flavin transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PS3_11827</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Flavin transferase that catalyzes the transfer of the FMN moiety of FAD and its covalent binding to the hydroxyl group of a threonine residue in a target flavoprotein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 10">
            <text>FAD + L-threonyl-[protein] = AMP + FMN-L-threonyl-[protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36847"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11061"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:74257"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.1.180"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Magnesium. Can also use manganese.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the ApbE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.180" evidence="2 8 10"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121651.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11827"/>
    <dbReference type="EnsemblBacteria" id="EHS87523">
        <property type="protein sequence ID" value="EHS87523"/>
        <property type="gene ID" value="PS3_11827"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.93"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017013">
        <property type="term" value="P:protein flavinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024932">
        <property type="entry name" value="ApbE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003374">
        <property type="entry name" value="ApbE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30040">
        <property type="entry name" value="PTHR30040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02424">
        <property type="entry name" value="ApbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006268">
        <property type="entry name" value="ApbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143631">
        <property type="entry name" value="SSF143631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0274">FAD</keyword>
    <keyword evidence="4 8 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 10" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR006268-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="301" mass="33302" checksum="018657151CF4F3A8" modified="2012-04-18" version="1">MIAEASTHLMGSTITIKIDHPDGEQLIADCFKQLADYQERFSANNDQSELMQLNQLAGIRPMQVDEDLYDLIKLAVRYSCRGGYFNVAIGPLVKLWHIGFQDAQVPTAEQIQTTLKLIDPHQIELDDQNHAVYLKQAGMEIDLGGVAKGYFADQLAIKLKAAGVESALINLGGSSIVTIGGNANNSDGQWHVGLQDPRGKRTDYATILALQDEAMTTSGVYERQLIKSGRTYHHILDSKTGYPIETSIMSLTVLGHQAVLGEILTTMCFGLEPSQMNDWLKEFPGITILTIDQQGEIRYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHX5</accession>
    <name>H4GHX5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endoribonuclease YbeY</fullName>
            <ecNumber evidence="7">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ybeY</name>
        <name evidence="8" type="ORF">PS3_7240</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the endoribonuclease YbeY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121659.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7240"/>
    <dbReference type="EnsemblBacteria" id="EHS87531">
        <property type="protein sequence ID" value="EHS87531"/>
        <property type="gene ID" value="PS3_7240"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.101"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00009">
        <property type="entry name" value="Endoribonucl_YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023091">
        <property type="entry name" value="MetalPrtase_cat_dom_sf_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002036">
        <property type="entry name" value="YbeY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020549">
        <property type="entry name" value="YbeY_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46986">
        <property type="entry name" value="PTHR46986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02130">
        <property type="entry name" value="YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00043">
        <property type="entry name" value="TIGR00043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01306">
        <property type="entry name" value="UPF0054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 7" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="156" mass="18031" checksum="C44C033974282A16" modified="2012-04-18" version="1">MELVIYDHTNGQVTDEQLDETEKLLQFAADQLSIAANAEMSVTYVNNPEMQKINREYRGIDRSTDVISFAIEDDEDLMLPAELQAELPRELGDLFVSIDKVKSQALFLDHSEERELGYMLVHGFLHLNGYDHERADDHGEQFRLQEEILTKYGLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHX7</accession>
    <name>H4GHX7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">GatB/Yqey domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_13395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121661.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13395"/>
    <dbReference type="EnsemblBacteria" id="EHS87533">
        <property type="protein sequence ID" value="EHS87533"/>
        <property type="gene ID" value="PS3_13395"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.103"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016884">
        <property type="term" value="F:carbon-nitrogen ligase activity, with glutamine as amido-N-donor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019004">
        <property type="entry name" value="YqeY/Aim41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042184">
        <property type="entry name" value="YqeY/Aim41_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28055">
        <property type="entry name" value="PTHR28055"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09424">
        <property type="entry name" value="YqeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="147" mass="16235" checksum="C0E54EB935741967" modified="2012-04-18" version="1">MSLLDQLTKDMVTAMKAKDKETLSVVRMLKVAVTNEQISLGHDLTPDDENAVLSREYKQRKESLAEFESAGRTDLVEKTQHELAVVEKYLPEQLTAEEITQIVKASIEQSGAESMRDMGKVMGIVMPQVKGKADGKLVNETVKAALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHZ8</accession>
    <name>H4GHZ8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aspartate carbamoyltransferase</fullName>
            <ecNumber evidence="5">2.1.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Aspartate transcarbamylase</fullName>
            <shortName evidence="5">ATCase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pyrB</name>
        <name evidence="9" type="ORF">PS3_11244</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>carbamoyl phosphate + L-aspartate = H(+) + N-carbamoyl-L-aspartate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000006">
        <property type="protein sequence ID" value="EHS87477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11244"/>
    <dbReference type="EnsemblBacteria" id="EHS87477">
        <property type="protein sequence ID" value="EHS87477"/>
        <property type="gene ID" value="PS3_11244"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.128"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF16">
        <property type="entry name" value="PTHR11405:SF16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="OTCace_N" evidence="8">
        <location>
            <begin position="11"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="OTCace" evidence="7">
        <location>
            <begin position="160"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="317" mass="36021" checksum="026BE61350FA4766" modified="2012-04-18" version="1">MMAKLLRLPYFTTVEGLTNEEVHALIERAEEFKHGEPSPKLDEPVYVTNMFFENSTRTHNSFAMAERKLGLQEITFDAGHSSVSKGESMYDTCLTMSAIGVNLAVIRDRANDYYRQLTTERPVNSQLDLGIVNAGDGSGQHPSQCLLDMMTIHEQFGHFDGLKVAIVGDITNSRVAKSNMQLLHRLGAQIYFAGPEYWFSHEFDAYGQYVQLDDILDQLDVLMLLRVQHERHDDQTEAQFDVQAYCDQYGINQARYDRLPDQAIICHPGPINRDVEIVSELVEAPKSFFYQQMQNGVFMRMAMLEAVMRGRHLGGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI00</accession>
    <name>H4GI00_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA-dihydrouridine synthase</fullName>
            <ecNumber evidence="10">1.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PS3_17918</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 10">Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 5,6-dihydrouridine in tRNA + NAD(+) = a uridine in tRNA + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:54452"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13887"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 5,6-dihydrouridine in tRNA + NADP(+) = a uridine in tRNA + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:23624"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13887"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 10">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the dus family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="AICN01000007">
        <property type="protein sequence ID" value="EHS87451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121684.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000007.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17918"/>
    <dbReference type="EnsemblBacteria" id="EHS87451">
        <property type="protein sequence ID" value="EHS87451"/>
        <property type="gene ID" value="PS3_17918"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.130"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001269">
        <property type="entry name" value="DUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024036">
        <property type="entry name" value="tRNA-dHydroUridine_Synthase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004652">
        <property type="entry name" value="tRNA_dU_NifR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006621">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00737">
        <property type="entry name" value="nifR3_yhdG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 10" id="KW-0288">FMN</keyword>
    <keyword evidence="9 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Dus" evidence="12">
        <location>
            <begin position="16"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00002790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS87451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="333" mass="36944" checksum="1D421DD1ABB30A24" modified="2012-04-18" version="1">MEWQIGNVNIPNQVVVAPMAGVMNSAFRVICKKFGAGYVVSEMISDRGIMYHNQKTLRMMNVEAVEHPMGIQIFGGTKETLVEGAKFVDQHTEADVIDINMGCPVNKVVNTDAGARWLLDPNKVYEMVSYVTDAVSKPVTVKMRIGWDDHHILAVENALAAEKAGAAAIAMHGRTRKQMYTGHANWDILKDVASELTIPFMANGDIRSAEDAKYVLDYTGADAVMIGRAAMGNPWMLTQTVHYLETGELLPEATPEEKITTAKEHLARLVELKGEYGGVREFRGQAAYYLKGIPRVAKTKVALMEAEEQATMNELFDQLIDQYHERLAKQAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI34</accession>
    <name>H4GI34_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">23S rRNA (Uracil-5-)-methyltransferase RumA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_9404</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000011">
        <property type="protein sequence ID" value="EHS87444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121718.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000011.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9404"/>
    <dbReference type="EnsemblBacteria" id="EHS87444">
        <property type="protein sequence ID" value="EHS87444"/>
        <property type="gene ID" value="PS3_9404"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.174"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030390">
        <property type="entry name" value="MeTrfase_TrmA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061">
        <property type="entry name" value="PTHR11061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01230">
        <property type="entry name" value="TRMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRAM" evidence="6">
        <location>
            <begin position="22"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="431"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="431"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="478" mass="54037" checksum="525AB68977E8F130" modified="2012-04-18" version="1">MPSKRYQQSPSRPTRRPIPPIEVAIGDRFPLTIKKLGINGQGIGYFKHKVCFVPQTIPGEIIVAEVTAVHEKYLEAKIHRLKKASPDRVPPKDDYAGLAGGFELEHLAYQRQLAYKRQVVLDSLAKFRPFGYQHYQVRPTIAAPHPYGYRNKAQFQIRQIDGQVVAGLYRANSHEVIDMVTCAVQMPATMQAMRQLVQWIQELQIPVYDEEANSGIIKTIAIRESRSTGEIQVTFITNSRKLPHKRLLLDKIQTELPAVRSVMQNFNPGDTPLIWGKETTKLAGDDYLTESLMGLDFHLSARSFLQLHPEQTETLYEEAAKAIDLTPNDTLIDAYAGIGTIGLSLASRVKRVLGMETIPEAVADANRNAALNEITNARYEVGTAEEVMPRWRQEGLTFDAIIVDPPRTGLDQVFIDQILQEKPHKFAYISCNMATLARNLTRLIPTYRVDYIQPIDMMPQTPRCEAVVKLVLNDHHHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI57</accession>
    <name>H4GI57_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell surface hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_6952</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121741.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87396">
        <property type="protein sequence ID" value="EHS87396"/>
        <property type="gene ID" value="PS3_6952"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.209"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010315">
        <property type="entry name" value="DUF915_hydro-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06028">
        <property type="entry name" value="DUF915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="288" mass="33425" checksum="54E247F399BDB8C5" modified="2012-04-18" version="1">MKNLKWYLITSLIGLVSISGIWLFIGSLHQDRPDRSDADFIQSSVPTFFLHGWKSSYHAEQQMVNYAKKQGVTTNIIVARVHRSGRVTLSQNLNSAAKNPIIMINFDDNHMRDYHTDAKWLRNVIVKVRQHYHFTRYNLVGHSMANMTIYHFILVYGNDHQLPKLQKIVDIAGNYNGILGKKHQPIHIKNHPNMKPQEMLPAYQQLLKLRQTFPKKQIKILNIYGDYDHSGTDGHVANASTLSLKYLLNNRAKSYRVIKITGYHANHSGLHNNLKVDRILVDFLWRKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI71</accession>
    <name>H4GI71_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Primosomal protein DnaI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_10563</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121755.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10563"/>
    <dbReference type="EnsemblBacteria" id="EHS87410">
        <property type="protein sequence ID" value="EHS87410"/>
        <property type="gene ID" value="PS3_10563"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.225"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009928">
        <property type="entry name" value="DnaI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002611">
        <property type="entry name" value="IstB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07319">
        <property type="entry name" value="DnaI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01695">
        <property type="entry name" value="IstB_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DnaI_N" evidence="2">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="IstB_IS21" evidence="1">
        <location>
            <begin position="99"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="310" mass="35602" checksum="510B237946B12376" modified="2012-04-18" version="1">MQSLSVAMMKRMQAQANNQEYQKTVERVFSDPQIQAFLNQHQAELTPESVQRDYSKLYEFFHENHLILQNQTSIAPGYQPKLLINAGQIDVAYVPSAQLRAEQRAKELRDRVTIISMPKWLPAANFDDFYTDEKQGSPSRNLALEEALKFCMNYSVDHFQPGLYLSGSFGVGKTYLLGATANELAKRNVKTTILHFPSFVVKMRQAIQNRTLNEERDQVSQAPILMIDDIGADVMTAWARDDLLAVILEYRMQAELPTFFSSNMTMNELENHLAEDNKDNVEPLKAKRIMQRIQYLSREIDVQGNNLRMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI86</accession>
    <name>H4GI86_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_16622</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16622"/>
    <dbReference type="EnsemblBacteria" id="EHS87425">
        <property type="protein sequence ID" value="EHS87425"/>
        <property type="gene ID" value="PS3_16622"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.244"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="2">
        <location>
            <begin position="161"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="273"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="320" mass="36034" checksum="E54C7EA6A136FB73" modified="2012-04-18" version="1">MQINQIEVGEPLTLFVLLKDVQARTARNGNPYLNITFSDQTGEISGNLWDVTPIQIDEFQPGRVVYLQGLGDQYQGQAQVNIQGLRLATNDEPADPNLYVQRVDLTDQELREALRPFFSAIKVDEFRIIVTDLMNHYGDQFFQYPAAKVNHHALVGGLAFHTLSILRLAQAVAEQYSGINRSLLYAGAMLHDLGKVIELSGPVATQYTIAGSLLGHISIVDGEIVQACQRKNLDAESEPVILLRHVILAHHGLLEYGSPVRPQLIEAEILHRLDELDASIVEINQAMKDVEIDDYSERVFSLENRRFFKDELQRDFKDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI90</accession>
    <name>H4GI90_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_12631</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the thioredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121774.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12631"/>
    <dbReference type="EnsemblBacteria" id="EHS87311">
        <property type="protein sequence ID" value="EHS87311"/>
        <property type="gene ID" value="PS3_12631"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.250"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="5">
        <location>
            <begin position="28"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="105" mass="12212" checksum="04B991E5147BF1BE" modified="2012-04-18" version="1">MAINVSPVNFEQALEGPITLVDFWADWCGPCKLMAPVLDELEAHYQDRVKFVRYDVEQDNSIPQRYKVMSMPSLVLFRDGVVKEKVTGIYNYQQLSHYLDRKLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIA1</accession>
    <name>H4GIA1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC superfamily ATP binding cassette transporter ABC protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_9219</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121785.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9219"/>
    <dbReference type="EnsemblBacteria" id="EHS87322">
        <property type="protein sequence ID" value="EHS87322"/>
        <property type="gene ID" value="PS3_9219"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.266"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="3"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="235"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="267"/>
            <end position="274"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="454" mass="50942" checksum="43D085282C0A5A87" modified="2012-04-18" version="1">MAIQINDLNFQYQDQTILKIPELTIPDGQFTLLFGPSGGGKSTLLKLIAHLYPKYRGHLEGQITGNDQVAMMFQDPSLQFALDTPQHELEFTLENLQIAPTEIPTRIQQALSFCQIENLKDRQIVTLSGGEQQLVALAVAVAIDATIILLDEPFASLDHHHRHWILEKLMQLRQQGKTIIIADHDLNDYQDYQPQIIQVGHGQAYYLSSDQIQALLKVQAIESPVALPDSTAPILEFKNFALQQGHQTLMEVSSLAVPTNKITLLTGESGSGKTSLFKSITKRLPYQGSLKFNHKEIAKLKSLGLNQQIGVIFQQASDQFINITVQEELDLSRQHGHHPYFTDENLQATLTDLGLDQLMERVVYSLSGGQQKKLQVLVMLMMGQPVLMLDEPLTGLDRHSAQQIINLISKCQAVYPQTMLIIIHQLALIDKLVDHHLHLANHRLTYQGVVAYEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIA3</accession>
    <name>H4GIA3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13630"/>
    <dbReference type="EnsemblBacteria" id="EHS87324">
        <property type="protein sequence ID" value="EHS87324"/>
        <property type="gene ID" value="PS3_13630"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.268"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="11"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="332" mass="37414" checksum="E82DEA75720BFF84" modified="2012-04-18" version="1">MRKLGQLEVSPIGMGCMGFSHGYGKVPETDVAIKAIQGAHDYGCTFFDTAETYGREQFYPGHNEQLVGQAIKPFRKEVVLASKIHISTEEYQANHDLYRTIKRHLLHSLKNLQTDYLDHYYLHRYNEEIGMAPVAEVMGKLIDEGLIRAWGVSQVGLNLLKTAHEITPVSAVQNLYNMVERSCESEIISYCLQEGIGCVPFSPIASGLLSGKITQTTKFEGDDVRKFVPQLQAENLAANQPIVDLLDRFSTSKHATKAQIALAWMLKKYPNVVPIPGSKNLQRIIENLGAWKVQLTDAEFKDLDDSLNQLKIYGHRGFEESQQHSFSNNWRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIB1</accession>
    <name>H4GIB1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_13743</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121795.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87332">
        <property type="protein sequence ID" value="EHS87332"/>
        <property type="gene ID" value="PS3_13743"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.276"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="155" mass="17296" checksum="1B0D58B0EFB645DE" modified="2012-04-18" version="1">MEMTVTELLTQLETERIQVASQGLTADQLTQENLQAGLEHVSSQLAAGYLAQFSWQVEMPVGDPVNVRLETNLINVPLIEAPRLDPKLLDQTALYEVKVYMVIEGDQVNASGLRIDELTTADELMPAVDNYVEPFKNWVTEHIATAINNRESVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIB7</accession>
    <name>H4GIB7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lactamase_B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_18056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121801.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18056"/>
    <dbReference type="EnsemblBacteria" id="EHS87338">
        <property type="protein sequence ID" value="EHS87338"/>
        <property type="gene ID" value="PS3_18056"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.289"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="47"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="357" mass="41030" checksum="21553CE3F768B926" modified="2012-04-18" version="1">MASPKNINDVTKEVWTANVFPEWGTYLNETIANKQVPEGSFAMWWTGCMGFWLKTHENTNIVVDLWNGTGKHTHGDGEMRKGHQMMRMNGGHEQQPNLRNRPYVLDPFAVKDVDALFVTHTHHDHLDIYTAASINKNCPNAKFVGPQGVVDEWLEWGIPKEKTVVVKPGDELEIGAVHVKALEAFDRTELVTEDDPNVKLAGTYPKDMSKIAVNYLFETSGGNLYDAADSHMSNTFTKHGNENKIDVAVCAYGENPRGVTDKLDDSQVLRMSEDLKTKVVIPMHWDIWSNFFTDPKDIFALWERKRYRLNYQFIPYILQVGGEFNYPQDKDKREYMYDRGFHDAFKHENDLPFPELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIC2</accession>
    <name>H4GIC2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative hexulose-6-phosphate isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_22229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121806.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_22229"/>
    <dbReference type="EnsemblBacteria" id="EHS87343">
        <property type="protein sequence ID" value="EHS87343"/>
        <property type="gene ID" value="PS3_22229"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.297"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016861">
        <property type="term" value="F:intramolecular oxidoreductase activity, interconverting aldoses and ketoses"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004560">
        <property type="entry name" value="L-Ru-5P_3-Epase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00542">
        <property type="entry name" value="hxl6Piso_put"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="24"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="294" mass="33833" checksum="E5607181B332481D" modified="2012-04-18" version="1">MTENALGIYEKALPQSLTWEEKFNLVHDLGFNFIEFSIDESDERLARLDWTREERQEFRDAMWKTNSRINTMMLSGHRRYPLGSKDPVIRQKSLEMMQKAIDLAVDLGIRNIQMAGYDVFYEEKTVNSRELFVENLKKCVEMAAKKMVMLSIETMDDLFLNSMEKIDYLKTMIHSPWLQCYPDLGNITAWPENNVAYDLETNVDNIAAVHLKDTKAVTPTSKGQFKNVPFGEGCVDFEGTLRTLARLDYSGSYTIEMWGGDNGDSDPLAEVRAAKQYFDDLFEKVGITQEPVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GID1</accession>
    <name>H4GID1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_6498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121815.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6498"/>
    <dbReference type="EnsemblBacteria" id="EHS87352">
        <property type="protein sequence ID" value="EHS87352"/>
        <property type="gene ID" value="PS3_6498"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.312"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="SSF140990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="248" mass="28061" checksum="8CFE7A01296A101C" modified="2012-04-18" version="1">MENLINIFFVILIWIIAIPTILSIALCYVGRLSKTATVRFGGFYGQIIFGGLGVMIHELSHLLMALLFRHHITGFRLLHIPRKNDPNDRALGYVNHTWNPQSFYQQVGNLFIGIAPLFGGIAALLATFRWLTPGMYDWWLTLMGSEHLVIANQTPWWQYLIAIILVIHISVGCFDLSRADLQNSKQGLIVALGLIVVITLILSLLMSASAFQTAIFSWFPVVYWAFALAIACNLLVLLAFYLMYLFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIE4</accession>
    <name>H4GIE4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_8085</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8085"/>
    <dbReference type="EnsemblBacteria" id="EHS87365">
        <property type="protein sequence ID" value="EHS87365"/>
        <property type="gene ID" value="PS3_8085"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.339"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="18"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="407" mass="43783" checksum="84B4723E7608BEF7" modified="2012-04-18" version="1">MKQVDANSSSMSANQKWVLASLSSGFMLENMDVMFLSFSLSAIIAQLHISGAAGGWIGTFTNLGMFVGGTLFGLLGDRIGRVKTFTYTIFIFAIATGLTFFAHNITSLYILRFIAGIGAGGEYGVGIALIAENFRASQIGRMTSIAAIGGQVGSILAALLAAWVVPTWGWNTLYLFGILPVILTYFVREHVQESDSFLKAQAQAKQNQERISFGRLFATPSLSLQTLGLMWMTIVQIAGYFGLMNWLPSIVQQQHGLSVSGSSYWMIATIIGMSLGMLVFGTVLDKLSPRWAFTIFLLGSAGVMFTILNAHTMVGMLLAGACIGFFSNGMFGGYGAVISLLYPTEIRSTANNVIMNTGRAVGGFSSVVIGLLIDHYSLTVVMGFLATLYILSFLVMISLPGLRKIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIF2</accession>
    <name>H4GIF2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein-tyrosine phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_12389</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121836.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12389"/>
    <dbReference type="EnsemblBacteria" id="EHS87373">
        <property type="protein sequence ID" value="EHS87373"/>
        <property type="gene ID" value="PS3_12389"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.348"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016311">
        <property type="term" value="P:dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026893">
        <property type="entry name" value="Tyr/Ser_Pase_IphP-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13350">
        <property type="entry name" value="Y_phosphatase3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="SSF52799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS87373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="262" mass="29832" checksum="7E1CA04EB2C63720" modified="2012-04-18" version="1">MTQRLLSINDGENFRDLGGYQTTDGRTVKWRKLIRSGALGRLSANDLTYLQQFNLTHDIDLRSPREVNSLPDRVPEGTHFHHEPVFGTDLTEASKRTSELEPVMNVKPNTGSQHMREVYRLMTELDSATKAYRNFFTYLLETPEDGAVIFHCQAGKDRTGIGAYLVLSTLGVDQKNIEQDYLLTNQTTKQWIDDRLNELKDQGATQTFIDNYAQLAGVSLDYLNSAIQEINTNYGSTQRFLRDALQLSATDLKDLRTLYLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIG2</accession>
    <name>H4GIG2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpsR</name>
        <name evidence="6" type="ORF">PS3_4923</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit. Forms a tight heterodimer with protein S6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the bacterial ribosomal protein bS18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4923"/>
    <dbReference type="EnsemblBacteria" id="EHS87283">
        <property type="protein sequence ID" value="EHS87283"/>
        <property type="gene ID" value="PS3_4923"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.360"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00270">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_S18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018275">
        <property type="entry name" value="Ribosomal_S18_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_S18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479">
        <property type="entry name" value="PTHR13479"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00974">
        <property type="entry name" value="RIBOSOMALS18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="SSF46911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00165">
        <property type="entry name" value="S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00057">
        <property type="entry name" value="RIBOSOMAL_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="71" mass="8458" checksum="C70C01F2483F5B99" modified="2012-04-18" version="1">MRRRRKVDYIAANHIEYIDYKDTELLRRFISERGKILPRRVTGTSAKNQRRLTIAIKRARIMGLLPFVAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIG4</accession>
    <name>H4GIG4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">30S ribosomal protein S6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpsF</name>
        <name evidence="6" type="ORF">PS3_4767</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds together with S18 to 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the bacterial ribosomal protein bS6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121848.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000017.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4767"/>
    <dbReference type="EnsemblBacteria" id="EHS87285">
        <property type="protein sequence ID" value="EHS87285"/>
        <property type="gene ID" value="PS3_4767"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.362"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00473">
        <property type="entry name" value="bS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00360">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000529">
        <property type="entry name" value="Ribosomal_S6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020814">
        <property type="entry name" value="Ribosomal_S6_plastid/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035980">
        <property type="entry name" value="Ribosomal_S6_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribosomal_S6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21011">
        <property type="entry name" value="PTHR21011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01250">
        <property type="entry name" value="Ribosomal_S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54995">
        <property type="entry name" value="SSF54995"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00166">
        <property type="entry name" value="S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="96" mass="11217" checksum="6EA3AB1167932D85" modified="2012-04-18" version="1">METRTYEITYIIRPDIEESAKSALVANFDKILSDNGATVVDSTDWDTRRFAYPIEKYTEGTYHIVNITTDTDEPLNEFDRLSKINDDILRHMIVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIG5</accession>
    <name>H4GIG5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA gyrase subunit A</fullName>
            <ecNumber evidence="8">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gyrA</name>
        <name evidence="11" type="ORF">PS3_8983</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the topoisomerase GyrA/ParC subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type II topoisomerase GyrA/ParC subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121849.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000017.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8983"/>
    <dbReference type="EnsemblBacteria" id="EHS87286">
        <property type="protein sequence ID" value="EHS87286"/>
        <property type="gene ID" value="PS3_8983"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.363"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034335">
        <property type="term" value="F:DNA negative supercoiling activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01897">
        <property type="entry name" value="GyrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005743">
        <property type="entry name" value="GyrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006691">
        <property type="entry name" value="GyrA/parC_rep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035516">
        <property type="entry name" value="Gyrase/topoIV_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03989">
        <property type="entry name" value="DNA_gyraseA_C"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101904">
        <property type="entry name" value="SSF101904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="TOP4c" evidence="10">
        <location>
            <begin position="13"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="811"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GyrA-box" evidence="8">
        <location>
            <begin position="528"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="8">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="838" mass="93130" checksum="C4C4F17DF144D55E" modified="2012-04-18" version="1">MADQELPGRITDVNLTSQMRNSFLDYAMSVIVSRALPDVRDGLKPVQRRILYGMNELGVTPDKPYKKSARIVGDVMGKYHPHGDSSIYEGLVRMAQDFSYRYMLVDGHGNFGSVDGDGAAAMRYTEAKMSKIAVEMLRDINKDTVDFQDNYDGTEKEPQVLPSRIPNLLVNGATGIAVGMATNIPTHNLGEVISAIHLLMDNPEATTEDLMKVLPGPDFPTGAVVMGKSGIRKAYETGQGSIVVRTKTEIEELKNGKQRIVVHEIPYMVNKAKLIERIAELAREKEIEGITDVNDETDREGMRIVVDVRRDASAEVILNNLYKMTLMQTTFNFNMLAIVDGAPKILSLKEILTNYLQHQEIVIKRRTEFDLKKAQNREHIVEGLRIALDHIDEIINIIRSSQTSDIAKQQLINNFGLSDRQAQAILDLRLVRLTGLEREKIEDEYNKLVAAIADYKDILAHRERIQKIIYDELLEIQAKFGDERRTELQVGDLNSIEDEDLIEEEEIIVTLTHSGYVKRLPVDEFKSQNRGGRGVKGMGVHNDDFIEHLISSSTHDSLLFFTNSGKVFAMKGYEIPEYGRSAQGIPIINLLGINGDEQISAVINVSHETQTDDDDRDLFFVTKEGTVKRTSVNEFKNIRSNGLKAINLHEGDELIHVEIIDQDQAMILGTRLGYSVSFKASAVREMGRSAAGVRGVRLREGDYVIGAAQLSPDSNVLVISEQGYGKQTPATEYPIKGRGGKGIKTVNITEKNGPLVGLTTVQGDEDLMLATNKGVIIRFSVESVSVTGRSAIGVHLIKMNDDSVVATMAKVDKEPTTDESTADQTTNETEEISPQTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GII2</accession>
    <name>H4GII2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ABC transporter permease and ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PS3_2971</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000018">
        <property type="protein sequence ID" value="EHS87277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121866.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000018.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2971"/>
    <dbReference type="EnsemblBacteria" id="EHS87277">
        <property type="protein sequence ID" value="EHS87277"/>
        <property type="gene ID" value="PS3_2971"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.384"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="5 9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="533"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="579"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="623"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="4"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="661" mass="71113" checksum="5F63E87FC95D1BA1" modified="2012-04-18" version="1">MAYLELKDIRKSYYLGKEEFPVLKGINLEFELGEFVSILGESGGGKSTLMNIIGGLDREFEGQVIVNGNALDHSQEKRMDEYRRDEIGYIYQAYNLINHLTVLENVKLSLDMTTLSNQEREQRALDLLERVGLAEHAKKYPNQLSGGQKQRVAIARALSSDPKVIIADEPTGALDAENTEDVLKILDEIAAEGRLVIAVTHSQHVADAGTRIVHLAEGQIDGDERIRPAYPVDDANHRLTSKELSFAVSMRTAYKHFKFHFGRNLLIVIGTAIGLFAVMLFSGLGTGVNGYVNKQVTDLVNPKAVVVTQYQKGSSSSGGTGASQQQAYAQMAAGTGKTNFKNSDINKIKKLAHVSSVQKLYSATSVTVAYNGKQATAQSLSSWTSSNSNGTIKNGSKPGVGEVVLDKSSVAKNLTKSKAKSLIGKTVTLSYQAQDQSGNTVTVSFKAKVSGISSSQNSASYVNATTLTQAMRDAGVKPAATALAVKADSMNSSKTVVKEINRIKTNGQRVFTATSVSSMISTIQTYIDLITNILAGIAAISLLVSALMIIVTMYMSVADRTKEIGILRALGESKRDIRRMFIAESLIIGIFSAVIATIVAFLAEVGANALLANIAKYNFIQIGFNHVILVFIIAIIISLLAAWLPARHAAALNPIDALAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIJ0</accession>
    <name>H4GIJ0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DEAD-box ATP-dependent RNA helicase CshB</fullName>
            <ecNumber evidence="5">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cshB</name>
        <name evidence="11" type="ORF">PS3_13771</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Probable DEAD-box RNA helicase. May work in conjunction with the cold shock proteins to ensure proper initiation of transcription at low and optimal temperatures.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DEAD box helicase family. CshB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000020">
        <property type="protein sequence ID" value="EHS87269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121874.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000020.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13771"/>
    <dbReference type="EnsemblBacteria" id="EHS87269">
        <property type="protein sequence ID" value="EHS87269"/>
        <property type="gene ID" value="PS3_13771"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.395"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009409">
        <property type="term" value="P:response to cold"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01494">
        <property type="entry name" value="DEAD_helicase_CshB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030881">
        <property type="entry name" value="CshB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Q_MOTIF" evidence="10">
        <location>
            <begin position="3"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="34"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="234"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="403"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="3"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="404"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="419"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="460" mass="53137" checksum="A23A46DBDB680322" modified="2012-04-18" version="1">MAGNFKDYHFPTFVNQAIQDLKFETPTPVQQKVIPQILKGKSVVGQSQTGSGKTHAFLLPIMAKLKADVQQVQVVITTPSRELAYQIYQAAKQINQFNPHPFTIHNYVGGTDKEHQIDQLGRKQPQIVIGTPGRILDLVKSQALDIHLANQFVIDEADMTLDMGFLNQVDQIAAYFPENLQMMVFSATIPQGLRPFLKKYMGNPEIEEIPTETVINPNVENWLMSTKGRDKNDLLYQLLTMGNPYLALVFANTRERAEEINRYLTDQGLKVALIHGGLEPRTRKRVMRQIYNLDYQYVVATDLAARGIDIEGVSLVINDDIPSDLEYFVHRVGRTGRAKMQGTAITLYAPAEDDLISKLEERGIKFIPKVIKDGQIITTYDRNRRRDFRHKQKELDPTIKGYVKKQKRKVKPGYKRKIKRRIKQDELQKRRLEQRHEFLKGKRQRQRQHRAERQNQRNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIJ3</accession>
    <name>H4GIJ3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_4098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000020">
        <property type="protein sequence ID" value="EHS87272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121877.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000020.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87272">
        <property type="protein sequence ID" value="EHS87272"/>
        <property type="gene ID" value="PS3_4098"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.398"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024529">
        <property type="entry name" value="ECF_trnsprt_substrate-spec"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12822">
        <property type="entry name" value="ECF_trnsprt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="197" mass="20829" checksum="AB2AEDCA894E5177" modified="2012-04-18" version="1">MKQREQIRRLTVRTLLIVLVVLQDLIPMFGNIPLGPLSITTLPVTVAVVAVVLGPKEGTIVGFVWGILTWVRAFVYPSSALAPLIFTNPLISVVPRMLIGLFAGYTFKWLVKVAPKALSAGVAGLIAAVTNTVLVLGGIYLFANTPAVAAGYHVGSGHLAQALMVIVTTNGVTEAITTAILTPLIALPVMVAVKRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIL9</accession>
    <name>H4GIL9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ</fullName>
            <ecNumber evidence="10">4.2.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase</fullName>
            <shortName evidence="10">(3R)-hydroxymyristoyl-ACP dehydrase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Beta-hydroxyacyl-ACP dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">fabZ</name>
        <name evidence="11" type="ORF">PS3_21668</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Involved in unsaturated fatty acids biosynthesis. Catalyzes the dehydration of short chain beta-hydroxyacyl-ACPs and long chain saturated and unsaturated beta-hydroxyacyl-ACPs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:13097"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="4.2.1.59"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the thioester dehydratase family. FabZ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.59" evidence="10"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21668"/>
    <dbReference type="EnsemblBacteria" id="EHS87214">
        <property type="protein sequence ID" value="EHS87214"/>
        <property type="gene ID" value="PS3_21668"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.430"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008659">
        <property type="term" value="F:(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008693">
        <property type="term" value="F:3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047451">
        <property type="term" value="F:3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004317">
        <property type="term" value="F:3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00406">
        <property type="entry name" value="FabZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013114">
        <property type="entry name" value="FabA_FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010084">
        <property type="entry name" value="FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30272">
        <property type="entry name" value="PTHR30272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07977">
        <property type="entry name" value="FabA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01750">
        <property type="entry name" value="fabZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="151" mass="16881" checksum="DA69FC4DB25B7581" modified="2012-04-18" version="1">MTQLAIMTAQEIMDLIPNRFPICYIDYVDEMEPDQNIVATKNVTINESFFRGHFPGNPVMPGVLIIETLAQAASILILKSPSFYKKTAYLGAVKNAKFRGMVKPGDVMKLHIKMLKTRSRMGIVDAVATVNDKKVCSAELTFIVADREEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIM2</accession>
    <name>H4GIM2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">UDP-glucose 4-epimerase</fullName>
            <ecNumber evidence="5 8">5.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_7768</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>UDP-alpha-D-glucose = UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:22168"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="5.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.2" evidence="5 8"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121906.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7768"/>
    <dbReference type="EnsemblBacteria" id="EHS87217">
        <property type="protein sequence ID" value="EHS87217"/>
        <property type="gene ID" value="PS3_7768"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.436"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05247">
        <property type="entry name" value="UDP_G4E_1_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005886">
        <property type="entry name" value="UDP_G4E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01179">
        <property type="entry name" value="galE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 8" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="9">
        <location>
            <begin position="4"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="331" mass="36535" checksum="0C6596D0F629B0AE" modified="2012-04-18" version="1">MSILVAGGAGYIGSHMVKQLVESGEEVVVADNLSTGHKKAINPKAKFYYGDIRDRAFLDRIFVNEDITAVVHFAAFSIVPESMQEPLKYFDNNTGGMITLLEAMRDFKVKYIVFSSTAATYGIPETMPIKETDPQQPINPYGLSKLMMEKMMAWADEAYGIKFVALRYFNVAGAAADGTIGEDHGPETHLVPIIMQVAQGKRAELSIFGDDYNTPDGTNVRDYVHVMDLADAHILALKYLEAGNPSNAFNLGSSTGFSNKEMLEAAREVTGQEIPAKIAPRRPGDPDSLVAASDKAREVLNWQPKYDDVHDIIATAWQWHSTHPKGYDDRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIP2</accession>
    <name>H4GIP2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">50S ribosomal protein L10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rplJ</name>
        <name evidence="7" type="ORF">PS3_5737</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 6">Part of the ribosomal stalk of the 50S ribosomal subunit. The N-terminus interacts with L11 and the large rRNA to form the base of the stalk. The C-terminus forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121926.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5737"/>
    <dbReference type="EnsemblBacteria" id="EHS87237">
        <property type="protein sequence ID" value="EHS87237"/>
        <property type="gene ID" value="PS3_5737"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.460"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05797">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00362">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022973">
        <property type="entry name" value="Ribosomal_L10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_L10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_L10P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11560">
        <property type="entry name" value="PTHR11560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="SSF160369"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="167" mass="18069" checksum="72CAFE400CE65727" modified="2012-04-18" version="1">MSEATIAKKAAAVEAAKEMLTNAETALVVDYRGLTVEEVTTLRKTLRESGVKMQVIKNKILQRAVEGTDYEGLNDVFVGPTAIVSSDEDAIAPAKILKKFADEHEALEVKGGFIEKSIKTLDEINEYATMPGREDLLSMLASALSDPMRKIARAVKAVADKAEEEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIP4</accession>
    <name>H4GIP4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sortase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_16861</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121928.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16861"/>
    <dbReference type="MEROPS" id="C60.006"/>
    <dbReference type="EnsemblBacteria" id="EHS87239">
        <property type="protein sequence ID" value="EHS87239"/>
        <property type="gene ID" value="PS3_16861"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.463"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06165">
        <property type="entry name" value="Sortase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005754">
        <property type="entry name" value="Sortase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042007">
        <property type="entry name" value="Sortase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023365">
        <property type="entry name" value="Sortase_dom-sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04203">
        <property type="entry name" value="Sortase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63817">
        <property type="entry name" value="SSF63817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01076">
        <property type="entry name" value="sortase_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="230" mass="26097" checksum="E77824EEF752436D" modified="2012-04-18" version="1">MKIKKWLYWLLVLMLAIVSLLLIFNQQVKDYLVDSYHPTVTRQSIKANTKKKASYDFSNVQDLNLQTVAKARANKESVKVIGVITIPDIKMTIPIAKGVDNTTLALAAGTMRADQQMGKGNYALAGHNMANGSKILFSPLYYHAKVGQKIYITDLDRVYEYRIYERKFIDATQVEVVNNTAERIITLITCNDDGSRRLMIRGSYVKSYTYNHAPAKVQKAFSQKYTNGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIS8</accession>
    <name>H4GIS8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 7">30S ribosomal protein S9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpsI</name>
        <name evidence="9" type="ORF">PS3_8081</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121962.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8081"/>
    <dbReference type="EnsemblBacteria" id="EHS87158">
        <property type="protein sequence ID" value="EHS87158"/>
        <property type="gene ID" value="PS3_8081"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.507"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00532_B">
        <property type="entry name" value="Ribosomal_S9_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023035">
        <property type="entry name" value="Ribosomal_S9_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_S9_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="PTHR21569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="116"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="131" mass="14290" checksum="4E35A62B7949C01E" modified="2012-04-18" version="1">MAQQVQYSGTGRRKDSSACVRLVPGSGKITMNGRAIEDYIPFANLRAIVNQPFAATKTEGQYDVLVNVRGGGFSGQAGATRHGIARALLAVDSDFRDVLKKAGLLTRDPRMKERRKPGLKKARKAAQFSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIT8</accession>
    <name>H4GIT8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translation initiation factor IF-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">infA</name>
        <name evidence="5" type="ORF">PS3_11860</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the 30S ribosomal translation pre-initiation complex which assembles on the 30S ribosome in the order IF-2 and IF-3, IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at any time during PIC assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IF-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003681606.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11860"/>
    <dbReference type="EnsemblBacteria" id="EHS87168">
        <property type="protein sequence ID" value="EHS87168"/>
        <property type="gene ID" value="PS3_11860"/>
    </dbReference>
    <dbReference type="GeneID" id="61200565"/>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.518"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04451">
        <property type="entry name" value="S1_IF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00075">
        <property type="entry name" value="IF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004368">
        <property type="entry name" value="TIF_IF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33370">
        <property type="entry name" value="PTHR33370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00008">
        <property type="entry name" value="infA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2 3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S1-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="72" mass="8218" checksum="6D712A07FCB31097" modified="2012-04-18" version="1">MAKADVIEVEGKVTETLPNAMFKVELENGVEILAHVSGKIRMHYIKILPGDRVRVEMSPYDLTKGRITFRFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIV5</accession>
    <name>H4GIV5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">50S ribosomal protein L2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rplB</name>
        <name evidence="8" type="ORF">PS3_6150</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase.</text>
    </comment>
    <comment type="function">
        <text evidence="5">One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121988.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6150"/>
    <dbReference type="EnsemblBacteria" id="EHS87185">
        <property type="protein sequence ID" value="EHS87185"/>
        <property type="gene ID" value="PS3_6150"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.537"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_B">
        <property type="entry name" value="Ribosomal_L2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Rbsml_prot_L2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005880">
        <property type="entry name" value="Ribosomal_L2_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_L2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_L2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_L2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="PTHR13691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01171">
        <property type="entry name" value="rplB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L2_C" evidence="7">
        <location>
            <begin position="124"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="211"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="255"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="281" mass="30556" checksum="AA9132C7EE60E4C6" modified="2012-04-18" version="1">MGIRKYKGTTNGRRNMNGYDFAEITKTTPEKTLLAPLKHKAGRNNSGRMTVRHRGGGTKRQYRIIDFKRIKDDVPATVKAIEYDPNRTANIALLVYADGQKSYILAPKGLKVGMTVQSGPEADIKVGNALPLKNIPVGTVIHNIELKPGKGGQLTRSAGTSAQLLGKEEKYVLVRLSSGEVRMILATCRATVGAIGNDEHGLIKIGKAGRKRYTGQRPHVRGSVMNPNDHPHGGGEGKAPVGLPSPLSPWGKKTVGKKTRSHKARSNKFIVRGRKRGPHTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIW4</accession>
    <name>H4GIW4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Deoxyribose-phosphate aldolase</fullName>
            <shortName evidence="3">DERA</shortName>
            <ecNumber evidence="3">4.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">2-deoxy-D-ribose 5-phosphate aldolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Phosphodeoxyriboaldolase</fullName>
            <shortName evidence="3">Deoxyriboaldolase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">deoC</name>
        <name evidence="4" type="ORF">PS3_13404</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>2-deoxy-D-ribose 5-phosphate = acetaldehyde + D-glyceraldehyde 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12821"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="EC" id="4.1.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; 2-deoxy-D-ribose 1-phosphate degradation; D-glyceraldehyde 3-phosphate and acetaldehyde from 2-deoxy-alpha-D-ribose 1-phosphate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the DeoC/FbaB aldolase family. DeoC type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.4" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121997.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13404"/>
    <dbReference type="EnsemblBacteria" id="EHS87136">
        <property type="protein sequence ID" value="EHS87136"/>
        <property type="gene ID" value="PS3_13404"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.549"/>
    <dbReference type="UniPathway" id="UPA00002">
        <property type="reaction ID" value="UER00468"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004139">
        <property type="term" value="F:deoxyribose-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009264">
        <property type="term" value="P:deoxyribonucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046386">
        <property type="term" value="P:deoxyribose phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00959">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00114">
        <property type="entry name" value="DeoC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011343">
        <property type="entry name" value="DeoC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028581">
        <property type="entry name" value="DeoC_typeI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889">
        <property type="entry name" value="PTHR10889"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10889:SF1">
        <property type="entry name" value="PTHR10889:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001357">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00126">
        <property type="entry name" value="deoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with acetaldehyde" evidence="3">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="220" mass="23385" checksum="9ADDCDF6CB08C29A" modified="2012-04-18" version="1">MKFNSLIDHTLLAPDATADQIDQVIQEAHDHHFHTVMINPYWVKKVHQALIDSDVLTACVIGFPLGANTTRVKLFEAQDALENGADELDMVMNIGEFKAGHDQMVKADIQAVVDAGHRVHKVVKVIIETCLLTDDEISRAAQLVASTGANFVKTSTGFSKQGATVQAVKLMHEAVNGTATAVKASGGIHNSVNAQAMVDAGATRLGVSHSLAVIGEKAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIX3</accession>
    <name>H4GIX3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Malate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_8985</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="5">Divalent metal cations. Prefers magnesium or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the malic enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122006.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8985"/>
    <dbReference type="EnsemblBacteria" id="EHS87145">
        <property type="protein sequence ID" value="EHS87145"/>
        <property type="gene ID" value="PS3_8985"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.565"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015884">
        <property type="entry name" value="Malic_enzyme_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001891">
        <property type="entry name" value="Malic_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000106">
        <property type="entry name" value="ME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00072">
        <property type="entry name" value="MALOXRDTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="malic" evidence="8">
        <location>
            <begin position="68"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Malic_M" evidence="7">
        <location>
            <begin position="260"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000106-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="541" mass="59563" checksum="444A3FE6942A5D9B" modified="2012-04-18" version="1">MGIGQARLNNPFLNKGTAFTMAERQTYGLIGLLPPRVKTIEEQAAEVYALYQSKDSQIEKRHLLMEVFNTNRTLFFYLMEMHINEFMPIVYDPVIAEAIEQYNERYLKPQDAVYLNVDHPELIESSLKNGANGRDIRLIVVSDAQGILGIGDWGVNGVDISVGKLMVYTAAAGIDPAQVLPISIDAGTDNEQLLNDPNYLGQRHHRISGSTYFDFIDQFVTSVEKLFPNSLLHFEDFGRGTAAKILDQYQDRILTFNDDIQGTGIIVLAGVLGALKIAKQELVDQKFLSFGAGTAGTGIAKMLYQELLRHGLTPDEAKKHFYLVDKQGLLFDDTEGLTDKQKAFTRQRSEFDNDRDLTDLKTIVQTVQPTVLIGTSTVHDAFTEEVIKAMAQHCERPIIFPISNPTKKIEANPVDLIKWTQGQALVAAGIPVDDFNYQGVSYHFGQANNALIYPGVGVGALAVQAEKINDEMLAAAAHALSNLVDSTESGAPVLPPVSQLRQFSKVVATAVAQSAIDQGIAAPQYHDAAQAVADFVWEAKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIY1</accession>
    <name>H4GIY1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Prephenate dehydrogenase</fullName>
            <ecNumber evidence="4">1.3.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_9410</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + prephenate = 3-(4-hydroxyphenyl)pyruvate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:13869"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="ChEBI" id="CHEBI:36242"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.3.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-tyrosine biosynthesis; (4-hydroxyphenyl)pyruvate from prephenate (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the prephenate/arogenate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.12" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122014.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9410"/>
    <dbReference type="EnsemblBacteria" id="EHS87153">
        <property type="protein sequence ID" value="EHS87153"/>
        <property type="gene ID" value="PS3_9410"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.580"/>
    <dbReference type="UniPathway" id="UPA00122">
        <property type="reaction ID" value="UER00961"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02153">
        <property type="entry name" value="PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0827">Tyrosine biosynthesis</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="7">
        <location>
            <begin position="2"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="8">
        <location>
            <begin position="295"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="364" mass="39839" checksum="354CCE7E65481CAC" modified="2012-04-18" version="1">MTKVLIKGLGLIGSSLARAIRLQHPDVQILGDDLNSASLDYAMQNQVIDQVVANWDQVAEMDVIIFAGPVSQIIKDIQDLSHQSLKTGVLITDVGSTKQTVMKAAQAFKDQAVNFIGGHPMAGSHKTGVWAGRANLFENAFYFQIPLNAASAQQLDKLQDLLAATNAKWLQVTAPQHDKIVAQISHVPHVIASALVNQTERTFQGDPLGMRLAAGGFKSITRIASADPTMWSAILLNNPTMIKEQLHQFQAELTTVETWIDQGDQAAIFDFFKQAKVSRDQLGPDKVGAVPGQYDLFVNIADQVGALADVTRRVSQAGLNLVNLHILEIREEIDGILQLTFSTANDREQARQVLRDYEIITKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIZ1</accession>
    <name>H4GIZ1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LL-diaminopimelate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_5144</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122024.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5144"/>
    <dbReference type="EnsemblBacteria" id="EHS87103">
        <property type="protein sequence ID" value="EHS87103"/>
        <property type="gene ID" value="PS3_5144"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.595"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="2">
        <location>
            <begin position="33"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="392" mass="43480" checksum="66E1571C1CDC1A73" modified="2012-04-18" version="1">MEFEQSKLLDQLPTQFFATLVEKVGAQIKAGVDVINLGQGNPDQPTPEYIVKSTQKWVANPKTHKYSPFQGLPFFKRAAANFYQEKYGVQLDPAQEVCILGGSKIGLVELPWALMNPGDLMLLPDPGYPDYWSGAALGEVKFETVPLTAEHDFLPDLKSIPEATAQKAKFFYLNYPNNPTGAVATKEFYTELVAWAKKYHVGIISDFAYGALGFDEADPVSFMEVPGAKEVGIEFYTYSKTFNMAGWRLGFAVGNADIIKALNLIQDHLFVSVFPALQKAAEDALNDPQRDLEIKKLNQLYKSRRDAFINAAAKIGWTTKAPQGTFYAWMPVPDGYTSESFADKLLYEAGVAVAPGNGFGKYGEGYVRVGLLIDPDRLEEAVNRIDQLHLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ03</accession>
    <name>H4GJ03_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Aldo/keto reductase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_18980</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122036.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18980"/>
    <dbReference type="EnsemblBacteria" id="EHS87115">
        <property type="protein sequence ID" value="EHS87115"/>
        <property type="gene ID" value="PS3_18980"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.614"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="PTHR43827"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="4">
        <location>
            <begin position="16"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="3">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="279" mass="31524" checksum="F1BF113372AB0504" modified="2012-04-18" version="1">MQNETYTLSNGLTIPKIAFGTWQIPNEAAAKAVETAINVGYRLIDTAAQYENETGVGEGIKNSQVDREELFVVTKIAPDVKSYEGAVEAINDSLDRLGLGYVDLMLIHAPKPWKELFHNSPKDYFEENLQVWKAMEEAYHAGKIRSLGVSNFTIPDMENIINHSEIKPVAEQIKTHIGNVDEEILTFCQKNGLQLMGYSPIATGQLIKDQRLVDMANKYQVTVPQLCIKYLIQRDILPLPKSTHKEFMEQNKQLDFTINAADMQTLIDFQDETTFVPND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ52</accession>
    <name>H4GJ52_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Branched-chain amino acid transport system carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_6945</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the transport system for branched-chain amino acids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the branched chain amino acid transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122085.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87077">
        <property type="protein sequence ID" value="EHS87077"/>
        <property type="gene ID" value="PS3_6945"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.694"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004685">
        <property type="entry name" value="Brnchd-chn_aa_trnsp_Livcs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30588">
        <property type="entry name" value="PTHR30588"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05525">
        <property type="entry name" value="Branch_AA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00796">
        <property type="entry name" value="livcs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="286"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="381"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="429"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="457" mass="49060" checksum="E192AF49B8F98412" modified="2012-04-18" version="1">MNDLTSRKLTWKEYLVVASLLFGLFFGAGNLIFPLHLGQLAGGHWVSATVGFLVTGVVLPLLSVLAIAVTRAEGVYDIGKPLGAGFAIVFMVLIHATIGPLFGTPRTASVSFTVGIAPFISKGNEGMALLIYSAVFFALAFFFAYRENSILSNVGRVLNPMFLALLFIMFLVAFLRPLGNPMTAQATSDYVHGAFVNGFLQGYNTMDAPAGLAFGVTVVHAVRQMGQKRDNDVARVVAKSGALGMAGVAVIYLLLIVAGAMSLGKFKLSADGGVAFTQIVNYYGGAFGQAVLAVLIAVTCLTTAVGLVAAFAQDFHKHFPQISYHMWLFLTTLASFLFANFGLDTLLAWSTPMLMFLYPLVLVLILLSVCSPLFHRDPWVYLFVVVFTVVPALGDMVVSFPSVVSASAFGKMVAAWRSMLPLSDLGMSWLVPALIGLVLGLLVHFAYGRKRVLSREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ56</accession>
    <name>H4GJ56_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_4079</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122089.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4079"/>
    <dbReference type="EnsemblBacteria" id="EHS87081">
        <property type="protein sequence ID" value="EHS87081"/>
        <property type="gene ID" value="PS3_4079"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.702"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="245" mass="25832" checksum="D1E5CC6F72646ED5" modified="2012-04-18" version="1">MQVFGLVIIGVLIGTFVIAMGGGGGAFYLGVLTALFGLSPVTAAATSLVTALPSIYVGVIGYWRHHRINFKIGNQMLFAAIPGVILGLLLSPWIPEKIYTWIIALLLIYFGVKMLMRRGQQRSLQHPQIAALSYGLLGGLMVGISGSSGGGPIIAGLLLMGESLFDATATSSYVLAGMCSLGALVHAASGMVDWSIGVPLMIGAVIGGLFAPTIVEYLDRYPISEQILRVVLALMLIFFGLKSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ68</accession>
    <name>H4GJ68_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamate 5-kinase</fullName>
            <ecNumber evidence="7">2.7.2.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Gamma-glutamyl kinase</fullName>
            <shortName evidence="7">GK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">proB</name>
        <name evidence="9" type="ORF">PS3_1756</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + L-glutamate = ADP + L-glutamyl 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14877"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the glutamate 5-kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.11" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122101.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_1756"/>
    <dbReference type="EnsemblBacteria" id="EHS87023">
        <property type="protein sequence ID" value="EHS87023"/>
        <property type="gene ID" value="PS3_1756"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.715"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00359"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004349">
        <property type="term" value="F:glutamate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04242">
        <property type="entry name" value="AAK_G5K_ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00456">
        <property type="entry name" value="ProB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041739">
        <property type="entry name" value="G5K_ProB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011529">
        <property type="entry name" value="Glu_5kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005715">
        <property type="entry name" value="Glu_5kinase/COase_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019797">
        <property type="entry name" value="Glutamate_5-kinase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000729">
        <property type="entry name" value="GK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01027">
        <property type="entry name" value="proB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00902">
        <property type="entry name" value="GLUTAMATE_5_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 7" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="8">
        <location>
            <begin position="10"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="178"/>
            <end position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="220"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="267" mass="28904" checksum="AE6FB29C8E194676" modified="2012-04-18" version="1">MAKLRQLKAKRIVIKVGTSTLITSNGKVNLRAIDQLSFVISGLVSQGKQVVLVTSGAIGVAMNEVGLEQRPKAIPSQQALAAIGQSRLITMYLERFTTYGQNISQILLTYDVLKDPETRQNALNSIEQLLEWGIVPIVNENDTVSVDEMDHQTTFGDNDQLSAKVADLINADLLIVLSDIDGLYDDNPHTNPDAKMMEEVHEITAAIWEVAGGKGSKYGTGGMQTKLLAAQQMLENGKAMVLINGQDPSQIFDVIAGIPVGTLFQKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ81</accession>
    <name>H4GJ81_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipoate--protein ligase</fullName>
            <ecNumber evidence="4">6.3.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_4785</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-lipoate + ATP + L-lysyl-[lipoyl-carrier protein] = (R)-N(6)-lipoyl-L-lysyl-[lipoyl-carrier protein] + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10500"/>
            <dbReference type="Rhea" id="RHEA-COMP:10502"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 2/2.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.20" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4785"/>
    <dbReference type="EnsemblBacteria" id="EHS87036">
        <property type="protein sequence ID" value="EHS87036"/>
        <property type="gene ID" value="PS3_4785"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.736"/>
    <dbReference type="UniPathway" id="UPA00537">
        <property type="reaction ID" value="UER00594"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016979">
        <property type="term" value="F:lipoate-protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019491">
        <property type="entry name" value="Lipoate_protein_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004562">
        <property type="entry name" value="LipoylTrfase_LipoateP_Ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12561">
        <property type="entry name" value="PTHR12561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10437">
        <property type="entry name" value="Lip_prot_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00545">
        <property type="entry name" value="lipoyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="8">
        <location>
            <begin position="37"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="351" mass="38860" checksum="C77AE32196F232D8" modified="2012-04-18" version="1">MEEGSQMFLIDTSRHGEPVYDPIVNQSLDNYLINDLRLPGHGLIMYINDPAVIIGRFQNAYAEVNLPYLKEQGIKLVRRSSGGGAVYHDRGNIVFENIVVGDLSNYGDFKAIGEPIAQALRQLGIASAEVRGRNDIAVDGKKCSGMAMVKSGDAYAAGGTILFDLDQTVARQVLTPDRGKLESKGIKSVDARITNLKPYLPTQYQTWTSDDFKDYLLCALFEVDQVADIPTYHLTDEDWAIIDQRIADHYGQAAWNYGKNPGYQRYVSKHFPIGTISFNFSVQEQKISAIKVFGDFFTTGDPTKLESVLLGCGFDHDHLRASLSQVNLAANLGHVTVDEIIDLLMEDTNGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ97</accession>
    <name>H4GJ97_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidase S74 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_20202</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000031">
        <property type="protein sequence ID" value="EHS87009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20202"/>
    <dbReference type="EnsemblBacteria" id="EHS87009">
        <property type="protein sequence ID" value="EHS87009"/>
        <property type="gene ID" value="PS3_20202"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.772"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030392">
        <property type="entry name" value="S74_ICA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13884">
        <property type="entry name" value="Peptidase_S74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51688">
        <property type="entry name" value="ICA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S74" evidence="1">
        <location>
            <begin position="418"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="518" mass="55295" checksum="5AF1F84889649134" modified="2012-04-18" version="1">MIRAKSAWDVATSATNAASSAQSDAKQATSSVAALLTSVDTLKGTVTGLQAESTMQASEIRNKVTRSDVTGMLTGYATQEYTQSIATQKADEWNLNLIKLKNDTQTSVTNLSATVDGIKGTVATKADQLTVTELSNLIQTKVSSGDFTSATTQLKNEINQRVQVGDVISQINQEAGGNTLIQVSNGKKSLILDAGNTIITGKAWIPGAAISDLSADKITSGTIATGALKVNLNQGLIDFRTGNGSGTYFTKDQVVFSKNNVEPGFIGIAGSPISNTTTNELSINYHGKNKGGFAVRDIDNLDRQGKLIVSNEDNRWEVHTNNATYSKLVVGNKDPYFEYHYGTGLQTDVINGLYLDLSGPSLKYKTVSQVTGTGINIHDALIDIHGDNTISAVGCQLRVQGWSWVAGYVEAMGHSLHSALSLKTNIEPLDTARSLALINQTDLTSFTFKSEIATGSNFRHFGPIIDDVNQVAQYNTPPEFIAATYGDAPARDDDNIIGALMGAVQELTKRLEKVEAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJC9</accession>
    <name>H4GJC9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alcohol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_2783</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000034">
        <property type="protein sequence ID" value="EHS86970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122162.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000034.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2783"/>
    <dbReference type="EnsemblBacteria" id="EHS86970">
        <property type="protein sequence ID" value="EHS86970"/>
        <property type="gene ID" value="PS3_2783"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.833"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PKS_ER" evidence="4">
        <location>
            <begin position="8"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="340" mass="35984" checksum="6A34BF27392485E7" modified="2012-04-18" version="1">MKAAVINDPVDGYVTVKDVQLRDLEPGEALVDMEYSGLCHTDLHVAAGDFGEKPGRIIGHEGVGRVSKVAPGVTSLKVGDRVSIAWFYKGCGHCEYCLTGRETFCREVVNSGYTADGAMAEQCIVPADYAVKVPEGLDPVQATSLTCAGVTVYKALKVADVKPGQWVSIVGAGGLGNLAVQFAHNVFGCHVVVVDGNEDKLQAAKENGAEVLINRHDDNVAEQIQAKVGGVHAAVVTAVAAEAFDQAVDSLRPDGRLVAVALPQGDMKLNIAKTVLDGIIVAGSLVGTRQDLAECSQFGAEGKVTPIVKTRKLEEINDMIQELKDNKVVGRNVVDFVHNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJD6</accession>
    <name>H4GJD6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bifunctional protein FolD</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="8">Methylenetetrahydrofolate dehydrogenase</fullName>
                <ecNumber evidence="8">1.5.1.5</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="8">Methenyltetrahydrofolate cyclohydrolase</fullName>
                <ecNumber evidence="8">3.5.4.9</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="8" type="primary">folD</name>
        <name evidence="11" type="ORF">PS3_11044</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5,10-methenyltetrahydrofolate + H2O = (6S)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.5" evidence="8"/>
    <dbReference type="EC" id="3.5.4.9" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000035">
        <property type="protein sequence ID" value="EHS86885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122169.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000035.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11044"/>
    <dbReference type="EnsemblBacteria" id="EHS86885">
        <property type="protein sequence ID" value="EHS86885"/>
        <property type="gene ID" value="PS3_11044"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.845"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="4 8" id="KW-0521">NADP</keyword>
    <keyword evidence="1 8" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THF_DHG_CYH" evidence="9">
        <location>
            <begin position="19"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="THF_DHG_CYH_C" evidence="10">
        <location>
            <begin position="136"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="180"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS86885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="301" mass="33016" checksum="B293B370884D7958" modified="2012-04-18" version="1">MLELKPEFRIRGDELVEILNGKAVAKNLNERTAKRVATLKERGITPGIAVIYVGDDPASAIYTRNKDKMALRLGMNSQLMHLPATATQEEVMAVLQEYNQDPMIHGILIQSPLPKHLDEHALVQAIRPDKDVDGFHSMNVGRLYNNEPGNYPVSCTPRGIMTMLDHYGIELAGKRVVIMGRSILVGRPLQALLINRDATVTMVHRHTKDPEAFTRQADILIVGIGQPQAVTSELVKPGAIVIDVGIHRTEKGKLIGDVDYDSVKEVAGMLTPVPGGVGPMTIASLMAQTVDLAEWSIRDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJE2</accession>
    <name>H4GJE2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">50S ribosomal protein L27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpmA</name>
        <name evidence="6" type="ORF">PS3_4114</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the bacterial ribosomal protein bL27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000035">
        <property type="protein sequence ID" value="EHS86891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122175.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000035.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4114"/>
    <dbReference type="EnsemblBacteria" id="EHS86891">
        <property type="protein sequence ID" value="EHS86891"/>
        <property type="gene ID" value="PS3_4114"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.852"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00539">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001684">
        <property type="entry name" value="Ribosomal_L27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018261">
        <property type="entry name" value="Ribosomal_L27_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893">
        <property type="entry name" value="PTHR15893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01016">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00063">
        <property type="entry name" value="RIBOSOMALL27"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00062">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00831">
        <property type="entry name" value="RIBOSOMAL_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS86891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="93" mass="9966" checksum="7753E0D46BA41D6C" modified="2012-04-18" version="1">MIMNLQFFSHHKGGGSTANGRNSAGRRLGTKAADGSFVTAGSIIYRQRGTHINPGTNVGRGGDDTLFAKTDGVVKFERLGRDKRKVSVYPVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJI0</accession>
    <name>H4GJI0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fructokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_13242</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000041">
        <property type="protein sequence ID" value="EHS86570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13242"/>
    <dbReference type="EnsemblBacteria" id="EHS86570">
        <property type="protein sequence ID" value="EHS86570"/>
        <property type="gene ID" value="PS3_13242"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.937"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="303" mass="33319" checksum="C5E03B094289502C" modified="2012-04-18" version="1">MQPLTRRIAMTTLLGSVEAGGTKFVCAVGNEDYQIEDQVTFPTTSPAETLGKTIEFFEKYRKDLKAISIASFGPIEIRRNSPKYGYVTSTPKPGWADTDFVGPIREAFDVPIYWTTDVNGSAYGEYVLSTLFNRRINSLVYYTIGTGVGAGAVVDGRFIGDVGHPEMGHIRLKRHPADLDFKGICPFHGDCLEGLVSGPTFEARTGKKGKDVPLTDPVWDIMAYYTAQAALNATLMFRPAQIIFGGGVVSEPFLDMVRDHFKDLMNEYVEVGDLKEYISMPLAENNGSATIGNFALALQEINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJI5</accession>
    <name>H4GJI5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitrogen fixation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_18742</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000041">
        <property type="protein sequence ID" value="EHS86575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122218.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000041.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18742"/>
    <dbReference type="EnsemblBacteria" id="EHS86575">
        <property type="protein sequence ID" value="EHS86575"/>
        <property type="gene ID" value="PS3_18742"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.944"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="PTHR10093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NifU_N" evidence="1">
        <location>
            <begin position="10"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS86575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="148" mass="16229" checksum="F37A9636F5B1AD03" modified="2012-04-18" version="1">MELNKLNQLYRELVLDHAEHPRHQGTVSDANCQMSMDNPTCGDELTVTIKVSPDQVITAIAFEGTGCTISQASASMMTSAVQDKTVDEALELAQIFSDAAIGKTVSKDQLNRLGDAQLLTSVMEFPARIKCTTLAWWTLQRALLKESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJK3</accession>
    <name>H4GJK3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Phosphoglycerol transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_5704</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LTA synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122236.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5704"/>
    <dbReference type="EnsemblBacteria" id="EHS86513">
        <property type="protein sequence ID" value="EHS86513"/>
        <property type="gene ID" value="PS3_5704"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.977"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008484">
        <property type="term" value="F:sulfuric ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012160">
        <property type="entry name" value="LtaS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005091">
        <property type="entry name" value="Mmb_sulf_HI1246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase" evidence="9">
        <location>
            <begin position="253"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="674"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="681"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="481"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR005091-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005091-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005091-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS86513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="696" mass="79001" checksum="62F20544DDE8DF6E" modified="2012-04-18" version="1">MRLLLQKIRRGLNTKLGFFLLVVFLFAIKSYWAYQTKFNLGVSGAVQEFLLAFNTIPATLLFIGIALYFRGRLSYWLMIIINALLSTWLFANILYYREFSDFITLNVIKGSGTSSGNLGTSLGQIIRPSDFFVYADVILLILLLAFHLIRIDMRYFKRRYSLTISALAFLLFGVNLGMAESDRSQLLTRTFDNNYLVKYLGLEAYTIYDGVKTTHTSMVRSQASSKDLTPILNYIKKNYAAPNMQYYGTIKGKNIIIVHLESFQQFLINYKVNGQEVTPNINSLYNSSNSISFSNFFNQVGQGKTSDAEMMLENSLYGLPEGSAMSTLGTSNTFQAAPALLEQKLGYTTASFHGDTPSFWNRDNTYKSFGYQYFFSKDFFPEVKDSDVGYGTKDKLFMKYAAHYLQQLPQPFYAKLITVTNHYPYLIDKKNTDFPELTTGDNTVDPYVQTAHYLDQAVGELLTYLDETGLRKNTVLVLYGDHYGISENHKDAIAKILGKKSVTNYDLAMWQKVPLIINGEGISGGINETYGGEIDVLPTLEDLLGISSNKYIQFGQDLLSSENDQIVPFRNGSWVTPQYTKYGGQYFYTKSGKQIKNPTAAQKKKFKAIQKQVTTELGLSDKVLNGDLLRFYKLKGFTKVNKKNFTYNVKKSLSSLKKIQEKLKTSLYYQNDKKDTDYSTDAPELTSSTSLKYPTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJL8</accession>
    <name>H4GJL8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Major facilitator transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_11362</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122251.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11362"/>
    <dbReference type="EnsemblBacteria" id="EHS86528">
        <property type="protein sequence ID" value="EHS86528"/>
        <property type="gene ID" value="PS3_11362"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.996"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="382"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="24"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS86528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="439" mass="47739" checksum="20050D335C46B598" modified="2012-04-18" version="1">MDVTNDQARSTTLPKFKADPVKTVILASLMGTAIEFFDFYAYGTASAAYFPSVFFPQMTPLLATILSLLTFGVAFVARPVGSFLFGHFGDQLGRKRTLVVSLMVMGVATVAIGFIPSYNSIGVWGALLLCLCRFVQGIGLGGEWSGAVLVATENAPADKRALYGAFPEVGAPIGFFLCNGLFFVLENVLTPAQMTSFGWRIPFWASSVLVFIGLYVRRRLQETPLFKLAQEQDNTTKSPLKEVFKSNWREIAKGTFIMGATYALFFTLTTWSLSYATTSLGFSHSEFLLLLMGAIVEFAALIMLTSILADKIGRKKVLLTASVALVVYSLVFPYFLEGQHNMFGMLLFLGLGFLVMGSLYGPVGAVLPELFPTKVRYSGAGITYNLAAVVGAAVAPSITTWLIAEYGLHSAGYYMLALAVVSVVAWLLTKETKDVDYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJM0</accession>
    <name>H4GJM0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA replication protein DnaD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_3783</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000043">
        <property type="protein sequence ID" value="EHS86435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122253.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000043.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3783"/>
    <dbReference type="EnsemblBacteria" id="EHS86435">
        <property type="protein sequence ID" value="EHS86435"/>
        <property type="gene ID" value="PS3_3783"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.999"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034829">
        <property type="entry name" value="DnaD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006343">
        <property type="entry name" value="DnaD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07261">
        <property type="entry name" value="DnaB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158499">
        <property type="entry name" value="SSF158499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01446">
        <property type="entry name" value="DnaD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DnaB_2" evidence="2">
        <location>
            <begin position="130"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="232" mass="26830" checksum="F4E80B874937C5A3" modified="2012-04-18" version="1">MVADFLHRYLKAGTTSISNLLLHHYHEIGMTTGQLMVYLELKSYLDRGVEQPSLKDIADHLGTSEDQVNQLIHEMVSHKLVTQRLVPTASGKDTAVYDFSGLIDQLTKIGDQNDPNTDTKEPTTSREEIFKQIQVEFGRTLSPMELQYVNQWIDQDHYSTNLIHLALKEAVVNGKYTLKYMDRILLRWQQSNLTTPQAIEADRQRYEQRRQSRANTGHEMTEKIPIFKLNDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJM3</accession>
    <name>H4GJM3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">3'-5' exonuclease DinG</fullName>
            <ecNumber evidence="5 6">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">dinG</name>
        <name evidence="8" type="ORF">PS3_14256</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">3'-5' exonuclease.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the helicase family. DinG subfamily. Type 2 sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="5 6"/>
    <dbReference type="EMBL" id="AICN01000043">
        <property type="protein sequence ID" value="EHS86438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14256"/>
    <dbReference type="EnsemblBacteria" id="EHS86438">
        <property type="protein sequence ID" value="EHS86438"/>
        <property type="gene ID" value="PS3_14256"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1002"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02206">
        <property type="entry name" value="DinG_exonucl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006310">
        <property type="entry name" value="DinG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006054">
        <property type="entry name" value="DnaQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Exonuclease_RNaseT/DNA_pol3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="PTHR11472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01407">
        <property type="entry name" value="dinG_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00573">
        <property type="entry name" value="dnaq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="252"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEAH box" evidence="5">
        <location>
            <begin position="467"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="290"/>
            <end position="297"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="938" mass="106864" checksum="A35A693266E3C78E" modified="2012-04-18" version="1">MDKHQSSKGPIYAVVDLETTGTSLTSGSRVIQIGCVLVQNQKVINQFESKVNPGMAIPQAVQQLTGIHNRDLKQAPYFDEIAPTIYALLTETTFVAHNVNFDFAFINMELERAGYPTLEIPAIDTVTLSQILMPTAESFRLRDLTKYLAIEHDNPHSAISDADATATLLIDLMKRLDQLPTPTLKRLQNMNLKLPLDTALMFDRALVQRQKQNYRLPDDLYMSGELVLHKVRPVQVENALKRRPYPHTKRQKLAFFGDQFQYWPIQAKLMNSIYNQFSHSDTPRQLVVEAGTGTGKTLGYLVPLSYLTYPHDQIIVSTATNVLQQQIQAITIPQLNALLPFKMTAVIVKGNQHYIHLGRFARSLSMTDDAPNVQLLKARILVWLLTTTTGDLDELNLNAMNSPFFAQIRHGGLRTLTPDEPYYRDDFLVRRQRALQFANIIITNHAYLVAHADELTNDDQQAYLVVDEAQHLSQSVMQQSRTTISFQRVATDINQLHNLVSHRDEHNLTSVFKNLPLGVYNVELLYDDLDQVSQAVTNLEEALMDLLPPSQSSTAQIEQPIDNQAFQKVINFDLPQMIELEQALASVQLHFTSLQRLFNGRRDSWLMIDRYLMSQFNSYYQSLIEFDQQLHEMSKTVQDQPAAAVFWLSVNQGNETSTMKLSGGLLEQNHFLSHHVYPYFSAMIFTGATLFTSHKSSYLYQHLDLVADQVRIKRFGSPFDFQQQTHLYIAKDALEPEQTSQYVDYLAQTIAEITIANPCQTMILFNSLVTIEQVYSRLRNTGLFDQRDILAQGITGNRDKILKQFATGTDSVLLGAASFWEGIDLPEDQLQLLIITRLPFDSPDQVMVKAQHEHLKAQGQSPFYQADLPRMIMRMRQGIGRILRTEDDYGITIVLDPRLINRRYGKSVLNMLPENLPVDQVATNQLANLVKKFLKNHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJM5</accession>
    <name>H4GJM5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Diphosphomevalonate decarboxylase</fullName>
            <ecNumber evidence="2">4.1.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PS3_20423</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the diphosphomevalonate decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.33" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000043">
        <property type="protein sequence ID" value="EHS86440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122258.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000043.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20423"/>
    <dbReference type="EnsemblBacteria" id="EHS86440">
        <property type="protein sequence ID" value="EHS86440"/>
        <property type="gene ID" value="PS3_20423"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1004"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004163">
        <property type="term" value="F:diphosphomevalonate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005935">
        <property type="entry name" value="Mev_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029765">
        <property type="entry name" value="Mev_diP_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041431">
        <property type="entry name" value="Mvd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18376">
        <property type="entry name" value="MDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015950">
        <property type="entry name" value="Mev_P_decrbx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01240">
        <property type="entry name" value="mevDPdecarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="9">
        <location>
            <begin position="90"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="MDD_C" evidence="10">
        <location>
            <begin position="178"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS86440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="329" mass="36064" checksum="E2F4EBEA13CC97FD" modified="2012-04-18" version="1">MASTARAHTNIALIKYWGKRDADLILPYTDSLSLTLDEFYTTTTVDFDSQLKQDHLIIDGQVISPDKSAKVARFMDLVRQMSGQKAFAKIKSINHVPMSAGLASSASAFAALAAAASRAAGLNLTPTELSRLARRGSGSATRSVFGGLVQWHRGTDDESSYAEPIMEKVDFGLEMIAIMINRQQKKVSSRGGMQASVDTSPYFEIWPKVVEQDLKAIKIAIQEHNISKIGEIAEANAMRMHALTLSAQPPFTYFEASTIKAINLINQLRQTGLNCYYTMDAGPNVKVIYDQKDRPQLLAALGQVFGQENLVVAQPGPGIEYLETIECED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJN0</accession>
    <name>H4GJN0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Guanine deaminase</fullName>
            <shortName evidence="5">Guanase</shortName>
            <ecNumber evidence="3 5">3.5.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Guanine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_8987</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>guanine + H(+) + H2O = NH4(+) + xanthine</text>
            <dbReference type="Rhea" id="RHEA:14665"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Purine metabolism; guanine degradation; xanthine from guanine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the metallo-dependent hydrolases superfamily. ATZ/TRZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.3" evidence="3 5"/>
    <dbReference type="EMBL" id="AICN01000044">
        <property type="protein sequence ID" value="EHS86253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122263.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000044.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8987"/>
    <dbReference type="EnsemblBacteria" id="EHS86253">
        <property type="protein sequence ID" value="EHS86253"/>
        <property type="gene ID" value="PS3_8987"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1012"/>
    <dbReference type="UniPathway" id="UPA00603">
        <property type="reaction ID" value="UER00660"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008892">
        <property type="term" value="F:guanine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006147">
        <property type="term" value="P:guanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014311">
        <property type="entry name" value="Guanine_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02967">
        <property type="entry name" value="guan_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="6">
        <location>
            <begin position="67"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU366009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS86253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="440" mass="48391" checksum="F1CDBCC319A650BF" modified="2012-04-18" version="1">MIKTVLQGDFYTAVDPQTVAYHAQALIVIDQSGMIERIIESTDTDYQTSRQAAQAKDQLVEVPKGQVILPGFIDLHLHAPQWPQAGLALDKPLANWLNDYTFPLEAKYRDLAYAKQVYPDLVQNLLANGTTTVLFFGTIHNPANLVLAQACMDHGLRALIGRVAMDNPEQTPDYYRDASSQVALQATEDFIQQLTALGQGQALAPIPVITPRFVPSCTPTTLQGLGKLAAQYDLPIQSHLSESDWEHHYAIEHYGHHDANVLDHFGLLTDRAVMAHATQLTLTDEQLVADRHTAVAHCPISNAYFGNGTLATRRLMDRGIKLGLGSDISGGYSVSLYDNLRQAVTVSQMRTDGVTNQDQGEVDARISIRNAFYLVTVGGGQSLHLPIGQIAPGFQADLQLVQERPLLAPSDPIERLERLLYQTQSNHIQVVMVNGDFVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJN7</accession>
    <name>H4GJN7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Chromosome partitioning protein ParB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_20652</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ParB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000045">
        <property type="protein sequence ID" value="EHS86238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20652"/>
    <dbReference type="EnsemblBacteria" id="EHS86238">
        <property type="protein sequence ID" value="EHS86238"/>
        <property type="gene ID" value="PS3_20652"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1026"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041468">
        <property type="entry name" value="HTH_ParB/Spo0J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004437">
        <property type="entry name" value="ParB/RepB/Spo0J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17762">
        <property type="entry name" value="HTH_ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00180">
        <property type="entry name" value="parB_part"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="3">
        <location>
            <begin position="119"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="274" mass="30952" checksum="B9892995268B78FC" modified="2012-04-18" version="1">MSQHEAERVVQLPLSQIRPNPYQPRQIFDEKALTELATSIQTTGIFQPIIVRQPDPALTRYEIIAGERRFRATQLAGLTTIPAIVRVLNDRQMMEVAVLENLQRENLSPIEEAQAYQTLMERLDLTQAQVAHLLGKSRPYIANYLRLLGLPETVKQLVSKQELSMGQARTLLGLKKRSQITRVAKKVVQEGLTVRQLEQLVAKLNGERPTKPKAKTVETFSPYARELQNQLESHLGTKVNFSANDQLDHGKIEISYQSAAELERILTVLGISLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJP0</accession>
    <name>H4GJP0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative ribosylpyrimidine nucleosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_3970</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000045">
        <property type="protein sequence ID" value="EHS86241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122273.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000045.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3970"/>
    <dbReference type="EnsemblBacteria" id="EHS86241">
        <property type="protein sequence ID" value="EHS86241"/>
        <property type="gene ID" value="PS3_3970"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1029"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901564">
        <property type="term" value="P:organonitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023186">
        <property type="entry name" value="IUNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304">
        <property type="entry name" value="PTHR12304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="SSF53590"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IU_nuc_hydro" evidence="3">
        <location>
            <begin position="5"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="304" mass="32585" checksum="B19C90464C6E893D" modified="2012-04-18" version="1">MTIKIIMDTDPGIDDAAALAMALNNPEIDLKLITAVAGNVTVDKTTANALKIVHFFKKDVPVAAGAAQPLIKPFEDAARIHGESGMPGYDFGDNYGQPIAKPAVEALHDAIMAEDEITLVPTGSYTNIALLLAEYPEVKSHIKQIVAMGGSISGGNMTSVAEFNVFTDPDAASIMYQSGIPIVTVGLDVTLKALLTPATQAKLLSLGETGKMLHGLITHYNDGDNEQGHPMHDVNTIFYLLHPEAFTTKDLWVDIQTSGPAIGAMVADVRGAYHDGQTNAKVCVDIDAEQFNQWFIEQVEKIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJP3</accession>
    <name>H4GJP3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glutamine ABC transporter ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_11845</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000045">
        <property type="protein sequence ID" value="EHS86244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122276.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000045.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11845"/>
    <dbReference type="EnsemblBacteria" id="EHS86244">
        <property type="protein sequence ID" value="EHS86244"/>
        <property type="gene ID" value="PS3_11845"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1032"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015424">
        <property type="term" value="F:ABC-type amino acid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030679">
        <property type="entry name" value="ABC_ATPase_HisP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039085">
        <property type="entry name" value="ABC_ATPase_HisP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="7"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="247" mass="27414" checksum="20FA06F36BC07F69" modified="2012-04-18" version="1">MDNQYKIEVTDLHKSYGDNEVLKGISLQVKPSEVVCMIGPSGSGKSTFLRCLNKLEAPDSGHIIIDGYDIADPKTNIDLVRQNIGMVFQHFNLFPNMSVLENITLAPVQLKKMTKEEAKTAALKYLEMVGLSDKADANPTSLSGGQKQRVAIARALAMKPDVMLFDEPTSALDPEMVGDVLEVMQELAREGMTMVIVTHEMGFAKRVANRVIFFHDGVIREEGTPEEIFDHPQHPDTQNFLEKVLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJR0</accession>
    <name>H4GJR0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein UspA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_14963</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000047">
        <property type="protein sequence ID" value="EHS86210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122293.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000047.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14963"/>
    <dbReference type="EnsemblBacteria" id="EHS86210">
        <property type="protein sequence ID" value="EHS86210"/>
        <property type="gene ID" value="PS3_14963"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1063"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="3"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="143" mass="15875" checksum="8AFD12B2A02139C7" modified="2012-04-18" version="1">MTYQRILVGIDGSDNSTRAYDTGVALAKALNATLYLTRVVNRDRGMDVTFNVNDDFYQDLYVKAVQELVPLIEQAHDQGVQDVQAVPRLGDIRTLLASELPNEFNIDLIILGNTGINRVKAMLIGSHTGYVVRHSKCDVLIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJR1</accession>
    <name>H4GJR1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Pyridine nucleotide-disulfide oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PS3_20540</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="8">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000047">
        <property type="protein sequence ID" value="EHS86211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122294.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000047.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20540"/>
    <dbReference type="EnsemblBacteria" id="EHS86211">
        <property type="protein sequence ID" value="EHS86211"/>
        <property type="gene ID" value="PS3_20540"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1067"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016668">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 10" id="KW-0274">FAD</keyword>
    <keyword evidence="2 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 10" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="12">
        <location>
            <begin position="7"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="11">
        <location>
            <begin position="330"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="428"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="166"/>
            <end position="173"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="9">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS86211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="440" mass="48370" checksum="EB5DB96471A478C4" modified="2012-04-18" version="1">METIKNLVIGFGKGGKTLAKTLAQHGEAVMVVEQSSQMYGGTCINVGCLPSKNMIINSERHLSFAEGVAHQNALTDRLRNKNYHMLADEPTVEVVDGKARFIGNHQVEIELVNGDHRQVEAERIFINTGAQPVIPDIPGLTLGERIITSKEALSLPYQPQTMAILGAGHIGLEFASMYHQYGTDVTVINRHDHILNHYDRDVAEMVEQDLINAGIRLRMNTNLVQVEATADQVTLTFDNGESQTVDALLVASGRRPNTADLGLENTGIQLTSRGAIQVNERLETAVPNVWAIGDVNGGPQFTYISLDDFRIINNQLFGDQTRSTKDRFAVPASTFLTPPLSSVGLSEETARAQGYNIKVYQQAAAGIPKAHVMEETRGLYKVIVDQDTQEILGAVIYAAESHEVINIVALAMKTHQPYSVLRDMIYTHPTMAEVFNDLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJS1</accession>
    <name>H4GJS1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Lactacin F ABC transporter permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PS3_19858</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000047">
        <property type="protein sequence ID" value="EHS86221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122304.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000047.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19858"/>
    <dbReference type="MEROPS" id="C39.001"/>
    <dbReference type="EnsemblBacteria" id="EHS86221">
        <property type="protein sequence ID" value="EHS86221"/>
        <property type="gene ID" value="PS3_19858"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1079"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043214">
        <property type="term" value="F:ABC-type bacteriocin transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005897">
        <property type="entry name" value="Pept_C39_ABC_bacteriocin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005074">
        <property type="entry name" value="Peptidase_C39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03412">
        <property type="entry name" value="Peptidase_C39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01193">
        <property type="entry name" value="bacteriocin_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50990">
        <property type="entry name" value="PEPTIDASE_C39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="1 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="276"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C39" evidence="11">
        <location>
            <begin position="9"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="167"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="479"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="512"/>
            <end position="519"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS86221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="711" mass="79415" checksum="BD8BC00DA1D8FDE4" modified="2012-04-18" version="1">MKYAAYVAQVDESDCGVACLAMILKHYGSDYPIAKLRDLARTNQEGTNALALVKVAQKLGLATQAVQADLAILDDPAIQFPWIAHVVKDGVLLHYYVIFGADENQVYVADPDPKAGVHKMARADFEQEWSGVALLFEPSADYQVQVVTMTTLKDNFHLLKPFRGTLALTICWSILISVISIVGSLYLQVLIDDLIPRHRVNLLMIVSLGMIIAYLIYAILSYLRTMILMKVNMKVAGRINLDYLRSVLHLPLRFFMTRKTGEILSRFNDISKIIDALSSTVVTIFLDAGMLVLIGLFMAWQNYPLFLTTLISVPIYIVLVAIFNRRYTKLNYDQMESNARLNSSIIESIRGIETVKALNITDRLDEQIDQQFQSFLKQSYRYTQTETFQAAIKLFLQYGLTTVILLVGSLQVMRNQLALGQLMAFNMLLAFFIEPLQDIVNLQAKLKTAQVANQRLNEVLVIEPEPSDGLALNDESLSLDVERVSFAYTYGYPALRDVSLSIQPGEKLAIIGPSGSGKSTLAKMMVRFLTPDQGSIKVNQRDIRSMALADLRHRIGYVPQRSQLFSGTIAQNLRLANPDMTLEELSRACHLAQIDADITKLPLGYDTLIDEESNTLSGGQVQRLAIARALLNHPQILILDESTSSLDPLTESELLQSLMALPMTIVLVSHRLNVTTHLDRVVVLDHGQIVQDGQPEDLQQVAGLYQQLLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJS5</accession>
    <name>H4GJS5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Homoserine O-acetyltransferase</fullName>
            <shortName evidence="3">HAT</shortName>
            <ecNumber evidence="3">2.3.1.31</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Homoserine transacetylase</fullName>
            <shortName evidence="3">HTA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">metAA</name>
        <name evidence="5" type="ORF">PS3_22116</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Transfers an acetyl group from acetyl-CoA to L-homoserine, forming acetyl-L-homoserine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine</text>
            <dbReference type="Rhea" id="RHEA:13701"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57716"/>
            <dbReference type="EC" id="2.3.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-acetyl-L-homoserine from L-homoserine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MetA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.31" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_22116"/>
    <dbReference type="EnsemblBacteria" id="EHS86112">
        <property type="protein sequence ID" value="EHS86112"/>
        <property type="gene ID" value="PS3_22116"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1094"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00074"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004414">
        <property type="term" value="F:homoserine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008899">
        <property type="term" value="F:homoserine O-succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00295">
        <property type="entry name" value="MetA_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033752">
        <property type="entry name" value="MetA_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20919">
        <property type="entry name" value="PTHR20919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04204">
        <property type="entry name" value="HTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000450">
        <property type="entry name" value="H_ser_succinyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3 5" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="3 4">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for acyl-CoA specificity" evidence="3">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="3">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000450-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS86112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="280" mass="32306" checksum="2D012157614E28C2" modified="2012-04-18" version="1">MTVYSLNGYTKDIDQSISGAFRIAILNLMPTKETTENQYRRVLAALETDVELTFFYPASHHFKSDQAATIKECYATWQEIADQHFDGLIITGAPVETVDFEEVDYWDEFCQIIDWSRDHCTKVLCLCWAAQAGMYYEADLPKYPTDQKLFGLYPVTVFHESELFDGIDVDRLKIPFSRHTTNRIMNIERTNLVLIAANEVVGPQIVESADHHFTYITGHPEYDRLTLYQEYHRDSEAGKSIELPANYFKGTPEPENVQDSWHQDALKLYKNVFKGCFAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJS7</accession>
    <name>H4GJS7_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cystathionine beta-lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_13731</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13731"/>
    <dbReference type="EnsemblBacteria" id="EHS86114">
        <property type="protein sequence ID" value="EHS86114"/>
        <property type="gene ID" value="PS3_13731"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1096"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="PTHR11808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS86114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="395" mass="42870" checksum="2FC9B913BF9D4A94" modified="2012-04-18" version="1">MGAFFILEEKNMTGFNTRLVHVPAPKDNQTGAVTVPIYKATTYEYPEIGAQVKYDYSRSGNPTRQALEDQVADLEGGKHAFAFASGMAAIHAALAIFKEGDHLIVGDQIYGGTFRLLNQFFNRWGLSISTVDTRDLAAIEAAIQPNTKGIYFEPVTNPLLQVTSVEEVAKLAQQHHLTTIVDNTFLSPYLSQPLTQGADVVIQSATKYLSGHSDVSAGTVTVKDDQYAERIYFVQNALGGILSPEDSNELARGIKTLSLRMDRQMENLKAIVEFLESQSVVSKINYPGLPGYPGYESLRTQAKGAGGVLSFELNDQADPVAFVNQLNYFKLAVSLGSVESLVELPSQMSHAELSPAEQLAAGIKPGLIRLSVGIEDAEDLIADLKQALLSAQRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJS9</accession>
    <name>H4GJS9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transport system membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_16319</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS86116">
        <property type="protein sequence ID" value="EHS86116"/>
        <property type="gene ID" value="PS3_16319"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1104"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043429">
        <property type="entry name" value="ArtM/GltK/GlnP/TcyL/YhdX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614">
        <property type="entry name" value="PTHR30614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="176" mass="19965" checksum="90C6FCE2BA0CADEE" modified="2012-04-18" version="1">MYSFKATNWFVRLYLLIFRGFPTLVILFVAYFGIPELLNTGSSVSPLAAAIFCFALKEAAYLEEIFRSGLSSVDGGQVEAGRSLGLSNQKVYRFIVIPQAIRIMIPNIGNTFIGLLKETSLAFSIGVTELFGEGKLIASENFQYFEVYIVVGIWYVILILIYTIFQQFLERRYSQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJX9</accession>
    <name>H4GJX9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC transporter ATP-binding membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_21815</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS85996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122362.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21815"/>
    <dbReference type="EnsemblBacteria" id="EHS85996">
        <property type="protein sequence ID" value="EHS85996"/>
        <property type="gene ID" value="PS3_21815"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1175"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="21"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="332"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="366"/>
            <end position="373"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="581" mass="64790" checksum="7B4123B8F30D028E" modified="2012-04-18" version="1">MIKIRLLKMLTSSRRQIALQIFWQWLSLIAQIMLIFAFASIFDQILHNKGKTISLLYALCTIGVAIIVRMGCSLAYDHASFFAASEIKQSLRFTMFEKLARLGTGYREQIPSVQITQMLTEGIEQLETYVGRYLSQFFYALLAPITLFIILVQINAPTSIVLLCAVPLIPIVIMVIMKLVTSILSTYFKSYYQLGDTFLEKLHGMTTLKVYQADQAAENEIIAESEQFRRITMKVLSTQLFSTIIMDLVAYGGAAVGIVVALSQYHQAHITLQETIVLVLLATEYFLPMRQLGAYFHMGMNGMKASNKIFEFLDLAEPTDGDQNIADQEFTISSHKLTFTYPHAHQPSLTNIALTIQPRQMIAFVGPSGAGKSTLVALLSGQARQYAGSLKFGPIELSDLSQDSLCDRITIVSHHSFVFKGSFRDNLILAKPDATDQELMQALKQVNLWDFCQSKAGLSTLIETDAANLSGGQKQRLAIARALLTDAQVFIFDEATSNIDQESEALIMQIIKQVAKTKTVILISHRLANVTDCDQIFYLDHGQIIESGDHQTLLAQNGAYANLFNHQAKLECFTSAKEELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK03</accession>
    <name>H4GK03_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tyrosine-protein kinase CpsD</fullName>
            <ecNumber evidence="4">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PS3_9719</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the regulation of capsular polysaccharide biosynthesis. Autophosphorylation of CpsD attenuates its activity and reduces the level of encapsulation. May be part of a complex that directs the coordinated polymerization and export to the cell surface of the capsular polysaccharide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:10137"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:82620"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Capsule biogenesis; capsule polysaccharide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CpsD/CapB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="4"/>
    <dbReference type="EMBL" id="AICN01000053">
        <property type="protein sequence ID" value="EHS85875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122386.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000053.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9719"/>
    <dbReference type="EnsemblBacteria" id="EHS85875">
        <property type="protein sequence ID" value="EHS85875"/>
        <property type="gene ID" value="PS3_9719"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1225"/>
    <dbReference type="UniPathway" id="UPA00934"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045227">
        <property type="term" value="P:capsule polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005702">
        <property type="entry name" value="EPS_synthesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01007">
        <property type="entry name" value="eps_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0270">Exopolysaccharide synthesis</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="AAA_31" evidence="14">
        <location>
            <begin position="63"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EHS85875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="251" mass="27416" checksum="CA8FBC28CDE62839" modified="2012-04-18" version="1">MGLFSRKKIQSVDTMVNGANLVTLTKPQSLVAEQFRTIKTNITFMGVDHPIKALAFTSSNISEGKSTVTANVGVTWAQDNKRVLMVDADLRRPTMHSTFDLDNRQGLTTLLMDQEHVINSQDVIQATEVPNLDVLTVGPVPPNATGLLNSQRMRDLVATFEQMYDVVIIDVPPILEVNDTQVLSSMLDGVVLVVRSGVTQKVAIKRAVEMLNISKANLLGYVMNDVASGDDDGYGYGYGYGYGYGTVEPED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK06</accession>
    <name>H4GK06_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Glucose-1-phosphate thymidylyltransferase</fullName>
            <ecNumber evidence="4 5">2.7.7.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_18759</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>alpha-D-glucose 1-phosphate + dTTP + H(+) = diphosphate + dTDP-alpha-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:15225"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37568"/>
            <dbReference type="ChEBI" id="CHEBI:57477"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.7.7.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the glucose-1-phosphate thymidylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.24" evidence="4 5"/>
    <dbReference type="EMBL" id="AICN01000053">
        <property type="protein sequence ID" value="EHS85878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122389.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000053.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18759"/>
    <dbReference type="EnsemblBacteria" id="EHS85878">
        <property type="protein sequence ID" value="EHS85878"/>
        <property type="gene ID" value="PS3_18759"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1228"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008879">
        <property type="term" value="F:glucose-1-phosphate thymidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045226">
        <property type="term" value="P:extracellular polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02538">
        <property type="entry name" value="G1P_TT_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005907">
        <property type="entry name" value="G1P_thy_trans_s"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43532">
        <property type="entry name" value="PTHR43532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01207">
        <property type="entry name" value="rmlA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="6">
        <location>
            <begin position="2"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="289" mass="32171" checksum="4ADBCE7679FDD5B8" modified="2012-04-18" version="1">MKGIILAGGSGTRLYPITHGISKQLIPIYDKPMIYYPLSTLMLAGIREVLVISTPDFMPLFENLLGDGSDLGMSFSYQVQEQPNGLAEAFILGENFIGDDSVCLILGDNIYYGAGLSQLVQSAAQKEIGATVFGYHVNDPERFGVVEFDDQMHALSIEEKPVHPKSNYAVTGLYFYDNQVVEIAKNIQPSDRGELEITDVNSEYLKSGQLDVKLMGRGYAWLDTGTHESMMEAANFIATVEKRQNLKVACLEEIAYRMGYIDQAQLVELAQPLKKNDYGQYLLRLAEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK46</accession>
    <name>H4GK46_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_15293</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000056">
        <property type="protein sequence ID" value="EHS85813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15293"/>
    <dbReference type="EnsemblBacteria" id="EHS85813">
        <property type="protein sequence ID" value="EHS85813"/>
        <property type="gene ID" value="PS3_15293"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1286"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025948">
        <property type="entry name" value="HTH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13276">
        <property type="entry name" value="HTH_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13333">
        <property type="entry name" value="rve_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="108"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="278" mass="32848" checksum="875C5E78DBE176A2" modified="2012-04-18" version="1">MSGMLKNLDLSKSNYYDWKKREPSDRGYKRGFVYDLIQKIYDQNKGIYGAPKICHELRKRRISISVRTVSLYMRQMGLRACYITPWTTTTRDPNFEASLQNILDENFNPDNPDEVWYIDTTYIPTDSGFVYLTSIIDLFSRKIVAWDTADTLSTEVVLQIIEKAKQRRNANKALIMHSDRGSQFTSEAYKKATNRMTNSYSAKGYPWDNACIESFHALIKREWLTRFRIKDIHEAHRLCFEYIDAYYNTVRIHGHCNYMSPMAFEQLYYENHHEQDVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK52</accession>
    <name>H4GK52_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ferric uptake regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_16410</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fur family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000057">
        <property type="protein sequence ID" value="EHS85805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122435.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000057.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16410"/>
    <dbReference type="EnsemblBacteria" id="EHS85805">
        <property type="protein sequence ID" value="EHS85805"/>
        <property type="gene ID" value="PS3_16410"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1295"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043135">
        <property type="entry name" value="Fur_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="PTHR33202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="149" mass="17228" checksum="A222B71AAD972A4F" modified="2012-04-18" version="1">MSEAEEYQHALDILHQNHVRLTSQRKLILNYLVTHHTHPSVEMIYDDLQDTDASISIATIYNTLNLLIDQHLVIELSNGDGSTHYDYFGNPHFHVVCDRCGKITDVFDDEYRDMELRLRQVAAKQTEYLVTKSDVEVHGICPECQQKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK77</accession>
    <name>H4GK77_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribonuclease J</fullName>
            <shortName evidence="9">RNase J</shortName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rnj</name>
        <name evidence="13" type="ORF">PS3_11008</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, may be a subunit of the RNA degradosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Bacterial RNase J subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11008"/>
    <dbReference type="EnsemblBacteria" id="EHS85529">
        <property type="protein sequence ID" value="EHS85529"/>
        <property type="gene ID" value="PS3_11008"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1335"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' exoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01491">
        <property type="entry name" value="RNase_J_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004613">
        <property type="entry name" value="RNase_J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042173">
        <property type="entry name" value="RNase_J_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030854">
        <property type="entry name" value="RNase_J_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041636">
        <property type="entry name" value="RNase_J_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17770">
        <property type="entry name" value="RNase_J_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004803">
        <property type="entry name" value="RnjA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00649">
        <property type="entry name" value="MG423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lactamase_B" evidence="12">
        <location>
            <begin position="23"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9 10">
        <location>
            <begin position="368"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="566"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR004803-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS85529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="593" mass="66347" checksum="A8AE5F0B6FBD43FF" modified="2012-04-18" version="1">MRKLKINNNEVAFFAVGGLGEIGKNMYAVQFQDEIVIIDCGVLFPEDDLLGVDYVIQNYDYLVENKDKIKGIFITHGHEDHIGGLPFFLQKVKAPIYSGPFAISLIKGKLEEKHLLRETEFHEVDEFSEVHFKKLWVSFFRTTHSIPDTLGIAVHTPQGTIVQTGDFKFDLTPVGNLPAPNMQQMAKLGEEGVLLLTSDSTNAERPEFTKSERWVDIHVRKVFQRIKGRIIFASFASNVYRLREASDEAIRQGRKIAVVGRSMENAIVAGRELGYLKIPDDALIDPSELNSYPPEQVMIMCTGSQGEPMAALSRIANGTHRQIAIQPGDTVVFSSSPIPGNTTSVNNLINRLEEAGANVVHGYVNNIHTSGHGGQVDEEIMLRLMKPKYFAPVHGEYRMQKIHTELAQLTGVPKQNTFILENGDVLALTNDSARIADHIESMGNVYIDGRDVNDSVGDPEIRERRMLSEEGVVTAIAVVDLKDFQIVAGPDIVSRGFVYMHESQDLIRAANHEVFWAILNTFKNHKNIDRRILNRTIVSRLQKLLYDRTGRHPVIIPMVTIINGDSGKSKHHKKGHSKKQEDKSKAETSVTAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK78</accession>
    <name>H4GK78_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Multidrug transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_12638</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the major facilitator superfamily. EmrB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122461.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS85530">
        <property type="protein sequence ID" value="EHS85530"/>
        <property type="gene ID" value="PS3_12638"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1336"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="231"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="451"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="9">
        <location>
            <begin position="15"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS85530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="492" mass="53659" checksum="66DA70DFC9529752" modified="2012-04-18" version="1">MQTDINGHSYNRTLLVIVLTIGTFCNFLTGTFLATAYPAIMRTFDISTSTVQWLTTSFMLVNGIMIPVSAWLINKLNSRVMYIVAESCFLLGTITAFVSPNFQTLLLGRIIQSIGVGVSMPLQQTIMLSIFPPNKRGAAMGIVGMATGLAPAIGPTLSGLVVDNLSWRDLFGFMIIPVIIVIALAFFLMRPVLKTHQQPLDVWSVITSTVGFGALLYGVSDASSRGWTDSLVLSFIIVGLIFIVLFVRRQFKLEDPFLKISVFKYPEFALASVISSVANLAMIGVEMLLPLYIQNVRGETAFHSGLMLLPGALMMGIMSPVTGRLFDRFGARYLALTGLTLLLVGTIPFLTLTNKTPVISIIIFYAIRMFGVTMVFMPVTTSGMNVLPLTDMSHGTAVNNTFRQALSSIGTAIMTTVLTNITNLTKPAHSVLVNTPLLYRHQYINATINGFHAAFATATLFALVAAVMACFLKSGNRTRDLNLDQIKPEVHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKD8</accession>
    <name>H4GKD8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hemerythrin HHE cation binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_4100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000063">
        <property type="protein sequence ID" value="EHS85502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4100"/>
    <dbReference type="EnsemblBacteria" id="EHS85502">
        <property type="protein sequence ID" value="EHS85502"/>
        <property type="gene ID" value="PS3_4100"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1429"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007380">
        <property type="entry name" value="DUF438"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04282">
        <property type="entry name" value="DUF438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF438" evidence="2">
        <location>
            <begin position="11"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="Hemerythrin" evidence="1">
        <location>
            <begin position="87"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="444" mass="50200" checksum="E4474500C29EDF51" modified="2012-04-18" version="1">MSQERQAAIVKILTFLHEGGDFDEAKQMFKDQFDQVDVAEITAAERQLIAGGLNPIEIQNLCNVHVALFEGQITDNQGTPAFQKPGHPVHTMKLENMVINSLVNDELLPCLKKWQQDGANNQYLARMRKALQDLKAIGRHYKRKELTIFPIMNKYGITAPPQVMWGVDDQIRDLIQRANDLVNQDSLPDKYEIEAAVTNAANEVTAMIFKEEDIMIPMLAEVASPEDWATARQDEGQFGYTLINPPLPWKPTQGEIEEAKAHPHESSIAKELNAAAIQMAQADSQAQGHLSTQEVYDQLNDKQLPAALTGDDVRIDLGSGTLSGRELKAIFPLLPLDLTFVDENDRVQWFTDTDDRIFPRTKSVLGRSVFECHPPRSVDKVKEIIAELHAGTRDRFEYWFNLHGERFVYVQYIGIHDADGQYLGCLECSQDISKFKELTGEQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKF4</accession>
    <name>H4GKF4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Efflux pump antibiotic resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_18253</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000065">
        <property type="protein sequence ID" value="EHS85428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122536.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000065.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS85428">
        <property type="protein sequence ID" value="EHS85428"/>
        <property type="gene ID" value="PS3_18253"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1470"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="330"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="10"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS85428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="454" mass="48595" checksum="C1C8EACCACCD4B14" modified="2012-04-18" version="1">MTKQTKNHLIILIAGMGMLLSTLDTGIINVALPFLQHQFHSTTSIAALSVVGYTTSLAVLILPFGYLSDQIGKLKVSLTGLLIFGFGSILCGLATNISGLIIFRIIQGIGAAALQATSAALITTLMDHDRVSKALGILGIMIGLGPILGPSIGGFFLALNVWRLIFWINVPFAILGLICNYLLIHQIHENTAKQRFDFSGSIINATMIICLLAGFSLLSKATSFKLAVPLIICGLVLGVCFYTFEFRLNEPLVDFRTLSKAPKIWLYLLQTAVFGFASAIVFLIPPFLFEKVMHIDVGVTGLLVLGAPVGLVIFSRVSGSQNDGSKNQKFSRWGLVVIALAFAGLLLVGVQWPPIVVTSCLFLFGVGGGYFQPANIGNIMQSGSANSQGTIGSLQRMVQNIAIASGTAIGSTLINLTSPNLFLGIQANWYLALFVVVIIVIADILINFLYSKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKF5</accession>
    <name>H4GKF5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Resolvase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_13406</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000065">
        <property type="protein sequence ID" value="EHS85429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13406"/>
    <dbReference type="EnsemblBacteria" id="EHS85429">
        <property type="protein sequence ID" value="EHS85429"/>
        <property type="gene ID" value="PS3_13406"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1471"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006120">
        <property type="entry name" value="Resolvase_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02796">
        <property type="entry name" value="HTH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0814">Transposable element</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="126" mass="14624" checksum="31FF619A37D781B0" modified="2012-04-18" version="1">MNTIQNKGATLDVLNLPSMTGIADPNLRQLMTNLIIELYKYQAESERKRIIERQQQGISLAKKQGKYHGRKPQYAEDDPRLLHAFKLYQAGMSDVDVARNTGIKRTTFIRYRKKYGIRGNREHPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKF8</accession>
    <name>H4GKF8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative resolvase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_9924</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the site-specific recombinase resolvase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000065">
        <property type="protein sequence ID" value="EHS85432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122540.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000065.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9924"/>
    <dbReference type="EnsemblBacteria" id="EHS85432">
        <property type="protein sequence ID" value="EHS85432"/>
        <property type="gene ID" value="PS3_9924"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1476"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006120">
        <property type="entry name" value="Resolvase_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02796">
        <property type="entry name" value="HTH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00398">
        <property type="entry name" value="RECOMBINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0229">DNA integration</keyword>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0814">Transposable element</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="8">
        <location>
            <begin position="2"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-serine intermediate" evidence="6 7">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR606118-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS85432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="194" mass="22387" checksum="5F8FFA2D70017C7B" modified="2012-04-18" version="1">MAKIGYARVSSKEQHLDRQLAALKDVDKLFTDKLSGANTNRPELQKMLAYIREGDIVLVTELDRLGRNNQDLTKIMNTIQNKGATLDVLNLPSMTGIADPNLRQLMTNLIIELYKYQAESERKRIIERQQQGIALAKQQGKYHGRKPQYTQDDPHLQHAFKLYQAGMSDVDVARNTGIKRTTFIRYRKKFNIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKI4</accession>
    <name>H4GKI4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">WelE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_14505</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial sugar transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122566.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14505"/>
    <dbReference type="EnsemblBacteria" id="EHS85264">
        <property type="protein sequence ID" value="EHS85264"/>
        <property type="gene ID" value="PS3_14505"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1525"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003362">
        <property type="entry name" value="Bact_transf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02397">
        <property type="entry name" value="Bac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Bac_transf" evidence="3">
        <location>
            <begin position="17"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="214" mass="24850" checksum="A4A75094750BD14D" modified="2012-04-18" version="1">MKTENKFQQKPSYLFFKRLTDIILSVVALVCFSPVFLAVWFKCQYGDNKGPVFFKQTRIGQYGRPFKIYKFRSMIVDADQVLHANQELYAKYVANNYKLEPEEDPRITRTGRFLRKTSLDEIPQFINIIRGEMSIIGPRPVVAEEVKIYGDRAEKFLSVKPGAMGLWQASGRSNIGYPERCDLELSYVDQASYWFDLKIMFKNIISIFTSEGAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKI5</accession>
    <name>H4GKI5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Regulatory protein RecX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">recX</name>
        <name evidence="6" type="ORF">PS3_19582</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Modulates RecA activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the RecX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19582"/>
    <dbReference type="EnsemblBacteria" id="EHS85265">
        <property type="protein sequence ID" value="EHS85265"/>
        <property type="gene ID" value="PS3_19582"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1526"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01114">
        <property type="entry name" value="RecX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003783">
        <property type="entry name" value="Regulatory_RecX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33602">
        <property type="entry name" value="PTHR33602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02631">
        <property type="entry name" value="RecX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS85265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="268" mass="31663" checksum="CF7E9770154301F3" modified="2012-04-18" version="1">MKMKKITKIEAQKRPGRYNIYLNGQYAFPVAESVLIKYRLAKDLELDTATLEQITKDDQIAKAYGRMLDYLSHQLRTEAEVKQKLIDLETPPEMIDPVMEHLRSNRLLDDQVYADSYVRTVMHTELKGPQVIRQKLRQKGVGELVIDQALEQFDLNNQLENAQKLAQRLVQRYRHEPTRRQEEKVRQGLMTNGYSSDLYNQLKEDLVLVNDEDQQASLLATSGEKIWQRNRRYENLYERKMKTKQALYRKGFDLDAIDRWLAERDSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKJ0</accession>
    <name>H4GKJ0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_9414</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122572.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000069.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9414"/>
    <dbReference type="EnsemblBacteria" id="EHS85270">
        <property type="protein sequence ID" value="EHS85270"/>
        <property type="gene ID" value="PS3_9414"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1534"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="252"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="298"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="374"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="409"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="433"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS85270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="612" mass="67384" checksum="C919D03C41F7096A" modified="2012-04-18" version="1">MWRYLKRLLIGKPLKTLDEGQANLTKFKGLALLSSDALSSVAYGTEQITTTLVALSTAAIWYSLPIAGIVLVLLFAITLSYRQIIRAYPNGGGAYVVATKNWGQNGGLIAGGSLLVDYMLTVAVSTTSGTEAITSAIPSLLPYSVPISVIIVIAIMMLNLRGLSESANFLTIPVYFFVIMIVVMIVWGLINIVTGHVAYNATADYGTPVAGMSAVLFIRAFSAGSSSLTGVEAISNAVPNFKEPKIKNAANTLAIMSLLLAAFFGGITFLSFYFGIVPNGRVTVLSQIGSAVFGGHGVAFYLLQLSTAMILAVAANTGFSAFPMLAFNMAKDKFLPHAFMDRGDRLGYSNGIIALAVGAIIMILIFHGETNMLIPLYAVGVFVPFTLSQSGMIIHWFREREGHWVSKSSINFVGAAISLALVCFLFWQHFWNVWPYLIIMPLLIWMFYKIHQHYLSVGEQLRVYEKTKVQLHDYDGATVIVLVGNVTRVTKGAINYARSIGDYVIAMHVSFDENPGKEHKTANEFKIEFPDVRFVDIHSSYRSVAQPTVRFVDIIAKRAADRNYSVTVLVPQFIPRKPWQRILHNQTSLRLRTILNSRENVIVSSYNYHLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKK9</accession>
    <name>H4GKK9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15">Dihydroxy-acid dehydratase</fullName>
            <shortName evidence="15">DAD</shortName>
            <ecNumber evidence="14 15">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ilvD</name>
        <name evidence="16" type="ORF">PS3_10152</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="15">Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13 15">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12 15">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the IlvD/Edd family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="14 15"/>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122591.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10152"/>
    <dbReference type="EnsemblBacteria" id="EHS85178">
        <property type="protein sequence ID" value="EHS85178"/>
        <property type="gene ID" value="PS3_10152"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1566"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="SSF143975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7 15" id="KW-0408">Iron</keyword>
    <keyword evidence="8 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="487"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="15">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="461"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="15">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EHS85178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="584" mass="62841" checksum="E53F3B10EAFF4347" modified="2012-04-18" version="1">MGTLSFSIDASKLQKRSDEITKGVDRAPGRSLLYATGMVKSPEDMDKPFIGICNSYIEIIPGHVHLRALADIAKEEIRKAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADSAETVINAHWFDGVLFMPNCDKITPGMLMAAARTNVPSVFVSGGPMKAGLDPNGKAMTLSNMFEAVGAFKDGAITKEEFLEMEMNACPTCGSCAGMFTANSMNCLMEVLGMALPYNGTALAISEQRKELVRQAARRVVEVVKENLRPRDIMTKEAFDDLYAADMAMGGSTNTVLHGLAVAHEAGIEYTEQDINTIAKRTPHIAKIAPSSSWTMEDVHNAGGMPAIMNELVELGGDVFHPDRPSVTGKTIRENVANHYSTNKEVIRPVENAYSTEGGLSVLYGNIAKKGSVIKSAGVDPDIKEFTGRAVCFDSHDEAVEGIDNGTVKKGNVVVIRYEGPKGGPGMPEMLNPTSDIIGRGLERDVALITDGRFSGATHGICIGHISPEAASGGEIALIKDGDQIHIDLTKRTLDLLVSDEEMEKRRQELPPFEPKVKSGYLARYQALVTSADTGGVMVGYDVLSKVSNLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKL6</accession>
    <name>H4GKL6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">UDP-N-acetylenolpyruvoylglucosamine reductase</fullName>
            <ecNumber evidence="16">1.3.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">UDP-N-acetylmuramate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">murB</name>
        <name evidence="18" type="ORF">PS3_11867</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 16">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>NADP(+) + UDP-N-acetyl-alpha-D-muramate = H(+) + NADPH + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:12248"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="ChEBI" id="CHEBI:70757"/>
            <dbReference type="EC" id="1.3.1.98"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 16">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 16">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 16">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the MurB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.98" evidence="16"/>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122598.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11867"/>
    <dbReference type="EnsemblBacteria" id="EHS85185">
        <property type="protein sequence ID" value="EHS85185"/>
        <property type="gene ID" value="PS3_11867"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1573"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008762">
        <property type="term" value="F:UDP-N-acetylmuramate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.78.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00037">
        <property type="entry name" value="MurB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003170">
        <property type="entry name" value="MurB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011601">
        <property type="entry name" value="MurB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036635">
        <property type="entry name" value="MurB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21071">
        <property type="entry name" value="PTHR21071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02873">
        <property type="entry name" value="MurB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56194">
        <property type="entry name" value="SSF56194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00179">
        <property type="entry name" value="murB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7 16" id="KW-0132">Cell division</keyword>
    <keyword evidence="11 16" id="KW-0133">Cell shape</keyword>
    <keyword evidence="15 16" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0274">FAD</keyword>
    <keyword evidence="8 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 16" id="KW-0521">NADP</keyword>
    <keyword evidence="13 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12 16" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="17">
        <location>
            <begin position="26"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="16">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EHS85185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="297" mass="31747" checksum="F6DFA3DF73FB69B6" modified="2012-04-18" version="1">MENLTSLFPTIEIKANDRLDQYTYTKTGGPADWVAFPQTFEEVQVLVRYANQQHLPLTVLGNASNLIVKEGGIAGLVLILTKLNHIQVSGEQVIAQAGASYIETTKVARDHALSGLEFAAGIPGSIGGAVFMNAGAYGGETKDVVQSATVISPVGEVTTLTHEELDFGYRHSSVQDTGSIVLSATFSLKTGDQEVISAMMDDLNQRRADKQPLDLPSCGSVFKRPVGYYAGKLIHDAGLQGYTVGGAQVSKKHAGFIVNIGQATATDYLEVIHHVQATVQAKFGVSLETEVRIIGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKP0</accession>
    <name>H4GKP0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Heat-inducible transcription repressor HrcA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hrcA</name>
        <name evidence="7" type="ORF">PS3_8454</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Negative regulator of class I heat shock genes (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HrcA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122622.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8454"/>
    <dbReference type="EnsemblBacteria" id="EHS84960">
        <property type="protein sequence ID" value="EHS84960"/>
        <property type="gene ID" value="PS3_8454"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1600"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00081">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002571">
        <property type="entry name" value="HrcA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021153">
        <property type="entry name" value="HrcA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023120">
        <property type="entry name" value="WHTH_transcript_rep_HrcA_IDD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34824">
        <property type="entry name" value="PTHR34824"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01628">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005485">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00331">
        <property type="entry name" value="hrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0678">Repressor</keyword>
    <keyword evidence="3 5" id="KW-0346">Stress response</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HrcA" evidence="6">
        <location>
            <begin position="104"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS84960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="350" mass="39583" checksum="6BE9EA1C0AC6319F" modified="2012-04-18" version="1">MLTKRQEEILRAVVRQYTTTGQPVGSKGLVDQLPMKVSSATVRNEMATLEKDGYLLKQHSSSGRIPSKHGYRYYVDNLLDQHAVTSNDLSVIQQSLGAEFHKIDEIISQSADILSKLTSYTTFTIRPDQREQHLGGFRMVPLGNHQVMGILVTDSGDVENQVFTIPAEINPETLEKVIRLINDQLKGLPLVEVMHRLQTDIPLKMARYLNQPEGFLDLFDQVLNQAARERFFVGGRTNLLGFTQEATPQQMQAIYQLMDADDQMAAILDQPNQNSHDIQVRIGQEIAGDQMLDHYSLITAKYNVEGYGQGLIAVLGPTRMSYSRTIGLVDAFRRELAKRLLDQYHHYHDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKP2</accession>
    <name>H4GKP2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_21034</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21034"/>
    <dbReference type="EnsemblBacteria" id="EHS84962">
        <property type="protein sequence ID" value="EHS84962"/>
        <property type="gene ID" value="PS3_21034"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1610"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="3"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS84962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="394" mass="42413" checksum="D896706ECFB2B425" modified="2012-04-18" version="1">MPTAISLYLNYFIHGMGVIILAQNMTSLAQKWGTNVGGVSMVISSLGIGRLIVLLISGYLSDKLGRKPFVILGAVTYILFFVGIVASSSVVIAYICGIIAGMANSFLDSGTYAGLMEMFPTKKGTSNVTLKAFISAGQFLLPLMVSFLVANHLWFGWSFVFCAVFLALNLCFMIPAGKFPPIGTDVEVEENGEEEEVTEQKGSLWFDGTLFILYGYISQTTFYLVSQWVSEYGSQVAHMGSTAAHSLVSYYSLGSIICVLVTAVLSAKAVKEVQFLSVYTLISFVALLLMYLFPTGVICSIMSFVVGFSAAGGVMQLGLTVMADFFPKGKGTVTGIFYTAGSIASFTVPIFTGMMASNLSNVILFDVIVALLGFVVTTLITIRYYKLFGKKAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKR0</accession>
    <name>H4GKR0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proline--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Prolyl-tRNA synthetase</fullName>
            <shortName evidence="9">ProRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">proS</name>
        <name evidence="11" type="ORF">PS3_14581</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122642.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14581"/>
    <dbReference type="EnsemblBacteria" id="EHS84980">
        <property type="protein sequence ID" value="EHS84980"/>
        <property type="gene ID" value="PS3_14581"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1633"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00861">
        <property type="entry name" value="ProRS_anticodon_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00779">
        <property type="entry name" value="ProRS_core_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01569">
        <property type="entry name" value="Pro_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004500">
        <property type="entry name" value="Pro-tRNA-synth_IIa_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023717">
        <property type="entry name" value="Pro-tRNA-Synthase_IIa_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044140">
        <property type="entry name" value="ProRS_anticodon_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033730">
        <property type="entry name" value="ProRS_core_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007214">
        <property type="entry name" value="YbaK/aa-tRNA-synth-assoc-dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04073">
        <property type="entry name" value="tRNA_edit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="SSF55826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00409">
        <property type="entry name" value="proS_fam_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="10">
        <location>
            <begin position="48"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS84980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="576" mass="63621" checksum="D912D0D67EA86161" modified="2012-04-18" version="1">MKQSRILMPTLKEAPSDAEAISHQMMVRAGYIYQVSAGVWAYLPLADRVIKKINQIIREEMDKIEAVEMMTPVLLPAELWQQSQRYESYGPNLFKLKDRKARDMILGPTHEETFTEIIRDSIHSYKKLPLVLYQIQDKLRDEDRPRYGILRGKEFIMKDAYSFTADQAGLDKVYDQMAVAYRNIFDRVGLDYKVIYADSGAMGGNGSQEFSAPAAVGEDTIAYTDGDYAANIEIAKSQFTGVQQTAATETMEKVATPGAHTVDEAAESLGLPASQVLKSMFYMATIDGEESPVMVLMRGNDEVNEVKVASHLKADELHLATEEEAEQLLGAHPGSLGPVGVGENVTILADNYVKVLVNMALGANDDGYHYRNANLDRDFRVDAFGDFRNVQEGELAPDGMPLKFTRGIEIGHIFKLGTRYSETLGAKVLDQNGRETDVIMGSYGIGISRLLSAIAEQNADDQGLVWPDSVAPFDVHVIPVNPKKTDQMELAEKLTQELESAGYEVLVDDRQERAGVKFADADLIGLPIRVTVGKKAAEGIVEIKLRRTGETLEVNAEELVTNIGILLKQAADTSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKR4</accession>
    <name>H4GKR4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosome-recycling factor</fullName>
            <shortName evidence="3">RRF</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Ribosome-releasing factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">frr</name>
        <name evidence="6" type="ORF">PS3_9394</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the RRF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122646.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9394"/>
    <dbReference type="EnsemblBacteria" id="EHS84984">
        <property type="protein sequence ID" value="EHS84984"/>
        <property type="gene ID" value="PS3_9394"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1637"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006415">
        <property type="term" value="P:translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00520">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00040">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002661">
        <property type="entry name" value="Ribosome_recyc_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023584">
        <property type="entry name" value="Ribosome_recyc_fac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036191">
        <property type="entry name" value="RRF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982">
        <property type="entry name" value="PTHR20982"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01765">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55194">
        <property type="entry name" value="SSF55194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00496">
        <property type="entry name" value="frr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRF" evidence="5">
        <location>
            <begin position="21"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS84984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="186" mass="20597" checksum="F479E15033F7A236" modified="2012-04-18" version="1">MTGKEILAEAKNKMAKSGDALKRTLADIRAGRANASVLNPVQVEYYGAMTPLNQLASISVPEARQLLVTPFDKSTLEDIEHAIYAANLGLTPQSDGTAIRLVIPQLTEERRKELVKDVKAELENAKVAVRNVRREAMDELKKGNKAGDFNEDEFHDLEKQVQTETDEGIKNLEAIAEAKEKELLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKR8</accession>
    <name>H4GKR8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lactate dehydrogenase related 2-hydroxyacid dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_14133</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122650.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14133"/>
    <dbReference type="EnsemblBacteria" id="EHS84988">
        <property type="protein sequence ID" value="EHS84988"/>
        <property type="gene ID" value="PS3_14133"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1644"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="4">
        <location>
            <begin position="7"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="5">
        <location>
            <begin position="112"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS84988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="332" mass="37662" checksum="6C0013B04070097C" modified="2012-04-18" version="1">MKILAYGVRDDEKPFLEEWEKQNPDVEVKAVPELITDETVEWAKGFDGVDVYQQLPYTDTVFEKLASYGIHNVSLRNVGVDNMNPEAVKKYDMRVTNVPVYSPAAIAEFSVTQLLNLLRRTKEYFHKFERNDYRWAPQISHELNDQVVGVIGTGHIGQVAINIYKGFGAKVIAYDVYHNPELEKEGLYVDTLEELYKQATVVTLHIPLFPTTEHMLNKEAFDQMKDGVFVVNASRGPLIDEKALIDALDSGKVAGAALDVLEDETKVFNHDMAGKNMDYPEFWNLNTRDNVLISPHTAFYTDVAVKNMVQYSLSANKDLIETGESDKLVKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKS6</accession>
    <name>H4GKS6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycerol kinase</fullName>
            <ecNumber evidence="5">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP:glycerol 3-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Glycerokinase</fullName>
            <shortName evidence="5">GK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">glpK</name>
        <name evidence="9" type="ORF">PS3_2999</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + glycerol = ADP + H(+) + sn-glycerol 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21644"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.30"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="5">Activated by phosphorylation and inhibited by fructose 1,6-bisphosphate (FBP).</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer and homodimer (in equilibrium).</text>
    </comment>
    <comment type="PTM">
        <text evidence="5">The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), including enzyme I, and histidine-containing protein (HPr) are required for the phosphorylation, which leads to the activation of the enzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the FGGY kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122658.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2999"/>
    <dbReference type="EnsemblBacteria" id="EHS84996">
        <property type="protein sequence ID" value="EHS84996"/>
        <property type="gene ID" value="PS3_2999"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1653"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00186">
        <property type="entry name" value="Glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="Carb_kinase_FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="Carb_kinase_FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005999">
        <property type="entry name" value="Glycerol_kin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01311">
        <property type="entry name" value="glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00933">
        <property type="entry name" value="FGGY_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="5 6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FGGY_N" evidence="7">
        <location>
            <begin position="6"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="FGGY_C" evidence="8">
        <location>
            <begin position="263"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="84"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="246"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="14"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="412"/>
            <end position="416"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphohistidine; by HPr" evidence="5">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS84996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="508" mass="56024" checksum="31C980280BF90319" modified="2012-04-18" version="1">MNNEQYILAIDQGTTSSRAIIFDHQGQEVAIAQQEFPQYFPKPGWVEHDALEIWDSVQSVVSEVMIKSQIKPYRIAAIGITNQRETTVIWDKATGQPIAHAIVWQSKQTSDLAEKLIADGYTELIHQKTGLVIDSYFSATKIKWLLDHVEGARERAQRGELLFGTIDSWLLWNFTGGQVHSTDVTNASRTMLFNIHDLDWDQDILDLLDIPRAMLPTVKESSAIFGYTGDYHFYGVQIPIAGIAGDQQAALFGQTAFQQGDVKNTYGTGAFTVMNTGIKPTLSQSGLLTTIAYGLNGQINYALEGSIFVAGSAIQWLRDGLAFFEHASDSEKLATEAESTGGVYVVPSFTGLGAPYWDQEVRGAMFGLTRGTSRAQIIRATLEAIAFQTRDVVDTMVKETQLPLSRLAVDGGASRNNFLLQFQANLLQTTIVRAQIEETTALGAAFLAGLAVDFWEDQAELVSLSHAGQQYQPKMSATDANQRYQGWQRAIKAVRFYSHGGLDDNSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKT3</accession>
    <name>H4GKT3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MFS family major facilitator transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_5624</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5624"/>
    <dbReference type="EnsemblBacteria" id="EHS85003">
        <property type="protein sequence ID" value="EHS85003"/>
        <property type="gene ID" value="PS3_5624"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1661"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="23"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS85003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="411" mass="44882" checksum="661D4B7BDCD64762" modified="2012-04-18" version="1">MTKEISIMETTLNEQTPEGWHQTFITLWIGCFITGMGYSMTMPFLSLFIAELGNFSRFQLNIYSGLAFAITFLSQAVISPYWGSLADQKGRKLMCLRASFVMSITIFLVGFATNVWVIILLRFLQGLFSGYISNATALMASETPHSRSGWVMTSMMTSGVTGNLVGPLLGGTLSGWFGYRIPFFITGILMFLTFLITFFLVHEHFSPIKKEELKPLKGIIAELPNVKIIVIMFFTTMFVQASVMSIDPIVSLYVKELMHGQGNISFVAGVVAATPGLGTLIASSKIGHTMDHIGPQKILIIGLAVAAALFVPMTFVSSPWALAFWRFILGLANAALLPAVQTILTLDTPADAFGRIFSYNQSFQAAGSVMGSLAGSTISGAFSYQAVFIVTGIMLLFNFILVIFNRTKSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKU4</accession>
    <name>H4GKU4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0246 protein PS3_4942</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_4942</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0246 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122676.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4942"/>
    <dbReference type="EnsemblBacteria" id="EHS85014">
        <property type="protein sequence ID" value="EHS85014"/>
        <property type="gene ID" value="PS3_4942"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1672"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00652">
        <property type="entry name" value="UPF0246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005583">
        <property type="entry name" value="YaaA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30283">
        <property type="entry name" value="PTHR30283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03883">
        <property type="entry name" value="H2O2_YaaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="250" mass="28616" checksum="93BC35C48848481F" modified="2012-04-18" version="1">MKYFISPSMKMKPGENFTSLQQELLFPKATSEVINSLQALNDQQRQNLWACSDRVAQEANEWTHQLSIDAPGSPAILTYQGIQYQAMAPDLMTEEQLERLADRLRIVSGLYGLVAPFVQIQPYRLEMKASLSVQARPDLYHFWGQRLYEKLFEDHDVVLNLASKEYSRAISPYLQTDDHLITIELLTDKHGKLTQPSTAVKQARGFFVRDLVERNVTTIEAVKDFSVAGYQFETELSSSTVFKFIRNGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKU5</accession>
    <name>H4GKU5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_16618</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122677.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16618"/>
    <dbReference type="EnsemblBacteria" id="EHS85015">
        <property type="protein sequence ID" value="EHS85015"/>
        <property type="gene ID" value="PS3_16618"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1674"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003797">
        <property type="entry name" value="DegV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043168">
        <property type="entry name" value="DegV_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02645">
        <property type="entry name" value="DegV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00762">
        <property type="entry name" value="DegV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51482">
        <property type="entry name" value="DEGV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="292" mass="32558" checksum="D6FB8E12D630EBE2" modified="2012-04-18" version="1">MKIAIVTDSTAYLEPDEIEKYQITVVPIPVIFGDQVYEEGVNLTNDEFYAKLKTGNVFPSTSQPSIGVLMETYEHLAQAGYDAVISIHLTSAISGLMATVTQLAEQLADTIKIIPFDSHLTIRMMGELAIEGARLAEQHDDLDDIIKKLSEYRDTFNNVFVVDDLQNLVRGGRLSNASAFVGSLLKIKPLLTMHTENYAIEAFEKVRSMKKAKLRCEEIFADDLKQLDYPVRAMVVHANAQTAGEEWLADLKGKYPDIRWDLTYFGPVIGTHLGSGALAIVWMQDTDKQALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKV7</accession>
    <name>H4GKV7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">ATP phosphoribosyltransferase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hisZ</name>
        <name evidence="8" type="ORF">PS3_906</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heteromultimer composed of HisG and HisZ subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="6">This function is generally fulfilled by the C-terminal part of HisG, which is missing in some bacteria such as this one.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122689.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_906"/>
    <dbReference type="EnsemblBacteria" id="EHS85027">
        <property type="protein sequence ID" value="EHS85027"/>
        <property type="gene ID" value="PS3_906"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1689"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00125">
        <property type="entry name" value="HisZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004517">
        <property type="entry name" value="HisZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="PTHR43707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA-synt_His" evidence="7">
        <location>
            <begin position="9"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="389" mass="44523" checksum="909B527BE8D2C936" modified="2012-04-18" version="1">MNQYRLPVGLKDYYGSRLDTKEQLRHYLFQRFDQLGYHKIQTPLLEYEPVFAEYETESRDLYRVLEADGNVLTIRPDLTLPIARFLSTTNVALPNKFSYVGEFMQRHREYQGFEREQTQAGIELVGYPSTKAEFECLTIINQINQDWFDNSLVIELGSVDLAEAVLDQVTKDHDQRRAIKQALFQKNLPVYEKLIEPYSAGPIGTFLKEWPWLFGSSAEIEELLKDSGLPAPIKKIIQQIIAFAQFITNLPGQSVMIDLASKAPQKYYTGLIFKAYTAQSNQYVISGGRYDQLLANVNSKNIPAVGMGINIQMMAELISPATPASRVEIYCQQDQLFAAQQLVNQHVNYELSLADSLREARSLAHQHQRQLMILNEKGELINDLENRTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKV9</accession>
    <name>H4GKV9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Carbamoyl-phosphate synthetase glutamine chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_13428</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CarA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122691.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13428"/>
    <dbReference type="EnsemblBacteria" id="EHS85029">
        <property type="protein sequence ID" value="EHS85029"/>
        <property type="gene ID" value="PS3_13428"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1693"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="SSF52021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CPSase_sm_chain" evidence="3">
        <location>
            <begin position="2"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00030197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="357" mass="39750" checksum="753A25BDA3D8CCCA" modified="2012-04-18" version="1">MPEKALILEDGTIFNGREFGSPAVSFGEVVFNTSMTGYQELITNQVYHNQIVVFTQPNVGNLPIQSNIYESIEPTIKGVVVRNAGMIDPDDRRSLDYYLKRHNIPGITDIDTRALVHHLRSLGRPVRGSLVPVADEHAFDQLNATVLTNQQVAQVSTPKPYPNPDVGPSVVVIDFGLKNGILRELSRRKCNVTVLPYDVTAEQIMQLDPAGVVLSSGPGDPHAIKSSVIEMIQKIQATVPLFAIGLGHELFALANETDSERLPIEYHGNNHPIQEIVTDHIIYAQQTMGYRIKRSAVNRQRLFITYVDLLTDSVIGLRHKYYPAFSVQFFPDGAPGPDDANPLFDEFVELLTKRKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKX1</accession>
    <name>H4GKX1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_4097</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000073">
        <property type="protein sequence ID" value="EHS84945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122703.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000073.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4097"/>
    <dbReference type="EnsemblBacteria" id="EHS84945">
        <property type="protein sequence ID" value="EHS84945"/>
        <property type="gene ID" value="PS3_4097"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1708"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS84945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="310" mass="35103" checksum="7596EAB1ECC9FBDC" modified="2012-04-18" version="1">MNKLSIIVPCYNEQESISLFYPAVEEVVNKIEGLEVEYWFVDDGSKDNSLAEMKQLHQEAPSRVHYVTFSRNFGKEAALYAGLQAATGDYVVVMDVDLQDPPSFLPEMYQILTTTDYDCVGTRRTTRTGETKLKSFLSDRFYDVINKISNTNIVNGARDFRMMTRQMVDAVLSMTEYDRFSKGIFSWVGFKTKYLEYQNIERVAGTTGWSMRKLFSYAMDGIIDFSQAPLSMAVWLGSGAFGLSILGIIVVIIRRLLVPSSSTFGWASLVCIILMIGGLQLLCIGIIGKYIGKIYMQVKNRPIYIIKEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKY3</accession>
    <name>H4GKY3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit beta'</fullName>
            <shortName evidence="8">RNAP subunit beta'</shortName>
            <ecNumber evidence="8">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">RNA polymerase subunit beta'</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Transcriptase subunit beta'</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rpoC</name>
        <name evidence="12" type="ORF">PS3_10545</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8 9">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000076">
        <property type="protein sequence ID" value="EHS84835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122715.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000076.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10545"/>
    <dbReference type="EnsemblBacteria" id="EHS84835">
        <property type="protein sequence ID" value="EHS84835"/>
        <property type="gene ID" value="PS3_10545"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1725"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01322">
        <property type="entry name" value="RNApol_bact_RpoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012754">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="PTHR19376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02386">
        <property type="entry name" value="rpoC_TIGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RPOLA_N" evidence="11">
        <location>
            <begin position="224"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1197"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="453"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="818"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="892"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="899"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="902"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS84835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="1218" mass="136112" checksum="7AAA9777F7993B11" modified="2012-04-18" version="1">MIDVNNFESMEIGLASPQKIRSWSFGEVKKPETINYRTLKPEKDGLFDERIFGPTKDYECACGKYKRQRFKGRVCDRCGVEVTSSKVRRERMGHIELAAPVTHIWYFKGIPSRMGLVLDMSPRSLEEVIYFASYVVLDAGDTPLEKKQLLSEPEYREKKAEYGDRFQAQMGAEALQKLLADVDLEKETAELKAVLKEATGQKRTRAVRRLDILEAFLKSGNRPEWMVMDVLPVMPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNAPGIIVQNEKRMLQEAADALIDNGRRGRPVTGPGNRPLKSLSHLLKGKQGRFRQNLLGKRVDYSGRSVIDVGPSMKMNQMGLPVQMAMELFKPFIIHELTKRELVNNPKAARRMIDNRDEVVFDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPILVSGKAMRLHPLVCAAYNADFDGDQMAIHVPLSDEAQAEARLLMLAAHHILSPRDGEPIVAPSQDMVIGNYYMTIEDKGREGEGMIFKDMNEAIMAYKNGYVSLQTRVGVQTSAYPKKPFTDDQRNRVMVTSVGKLIFNNIMPEDYVYINEPTMENLTNGVPDRYFLDKGEDINDYLENAPLVAPFKVGFLSDIIAEVYKKYKVTITSQLLDKMKDLGYYESTISGVTVGIVDVTDLKEKPEIIDEAHKQVALITKQFRRGLITDNERYERVIGVWNDAKDEVQEKLVEHMDIHNPINMMSDSGARGNISNFTQLAGMRGLMASPSGKIMELPILSNFREGLSVLEMFLSSHGARKGMTDTALKTANSGYLTRRLVDVAQDVIVREEDCGTDRGLDVTAITNGDEMIEPLYDRILGRYTMKSVFDPQTGEKIVGKNVLIDEAMAQEIVDAGVQKVTIRTAFTCNTDHGVCERCYGRNAATGDQVEAGEAIGTVAAQSIGEPGTQLTLRNFHTGGVAGDDDITQGLPRIQEIVESRNPKGKAVISEVTGVIESIEENPADQTKDVTVKGDADTRTYTLPITTRMRVGEGDEIRRGYALNEGSIDPKELIRVRDVLSTETYMLAEIQKVYRMQGIDLLDKHVEIMVRQMIRKVRVMDPGDTDVLPGELMDISQFRDANYQTLISGGVPATARPVILGITKAALETNSFLSAASFQETTRVLTDAAIRGKNDPLVGLKENVIIGKIIPAGTGMGTYRGIKPREVSVEQPETAYATTPTASIREMEAELNQED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKY7</accession>
    <name>H4GKY7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Transcriptional regulator CtsR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PS3_11348</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the CtsR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000076">
        <property type="protein sequence ID" value="EHS84839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122719.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000076.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11348"/>
    <dbReference type="EnsemblBacteria" id="EHS84839">
        <property type="protein sequence ID" value="EHS84839"/>
        <property type="gene ID" value="PS3_11348"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1729"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008463">
        <property type="entry name" value="CtsR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041473">
        <property type="entry name" value="CtsR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041908">
        <property type="entry name" value="CtsR_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040465">
        <property type="entry name" value="CtsR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041902">
        <property type="entry name" value="CtsR_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40028">
        <property type="entry name" value="PTHR40028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05848">
        <property type="entry name" value="CtsR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17727">
        <property type="entry name" value="CtsR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010607">
        <property type="entry name" value="Txn_repr_CtsR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CtsR" evidence="8">
        <location>
            <begin position="6"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="CtsR_C" evidence="9">
        <location>
            <begin position="81"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR010607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS84839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="156" mass="18048" checksum="FC7D297DBCC65D22" modified="2012-04-18" version="1">MAEEKSMSDVIEQYLKSVLGDASQIEIRRSEIADHFDVVPSQINYVIKTRFTPQRGYFVESKRGGGGYIRIEKVSLLDGVDVLETLINGIGDLLTERDAAAVIKTLYQEQLITRREGDLMMTMISKQTLIIRDRYDQQRFRAHVMKMLLNRLRYES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL19</accession>
    <name>H4GL19_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YycI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_18929</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122751.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_18929"/>
    <dbReference type="EnsemblBacteria" id="EHS84785">
        <property type="protein sequence ID" value="EHS84785"/>
        <property type="gene ID" value="PS3_18929"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1769"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018604">
        <property type="entry name" value="YycI-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09648">
        <property type="entry name" value="YycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="YycI" evidence="2">
        <location>
            <begin position="38"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS84785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="261" mass="29538" checksum="C88AB50F15FD0FF6" modified="2012-04-18" version="1">MNFRRIQGIFLIAFVILDIVLVISFFWGFWRVQSNSGQSVSQQAMLLRELKTDSITVPKLSNHQPRGYYISVNSSTDLGDEVTSLTRQSAYWSGTQITSTFNTALANQKSTIKSWLRSNSRIAHGQSYQVNQDFSTKSRLVYTQMIDGIPVLDATGRIQFRVSGQKLTGYTQTYLKGIKVLRQQVSAISSQQAVAVLYRHNEIPSNSKVIWLQYGFSKVSSSDNQDIYAPIWEAKVRDRSNNKTTYLRVNAITGTIIQTND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL21</accession>
    <name>H4GL21_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PS3_4572</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122753.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4572"/>
    <dbReference type="EnsemblBacteria" id="EHS84787">
        <property type="protein sequence ID" value="EHS84787"/>
        <property type="gene ID" value="PS3_4572"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1771"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029151">
        <property type="entry name" value="Sensor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103190">
        <property type="entry name" value="SSF103190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="16">
        <location>
            <begin position="202"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="14">
        <location>
            <begin position="259"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="15">
        <location>
            <begin position="328"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="13">
        <location>
            <begin position="383"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EHS84787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="622" mass="70007" checksum="39D320DECF002BA4" modified="2012-04-18" version="1">MNSKIKFYQSIQYKIALVFVLMLILTLEIVGALFVRQLEHENLTNFKQQIELPSYVENSLTQELIQTNTKTANRNINSTLQKVENNSITEIRVVDAKGIIRGTSNRDQSVVGQKANTKIVKNTLVNNRSHSETVYDSDSKIHYYVTTIPLMSGDTNNVVGVVYIRASLESVYSTVTSISLIYLSATVITIIIGLILSIIISREITRPIEEMRKQTLRIARGDFSGRVKVNGNDELGQLSAAINNLSIRVEESQESTDAERRRLDGVLAHMSDGVLATDRRGNVIIINNMALQLLGVDDEKEVIGQSILGVLDIRKNYSIRDLVENEQAQNEVILDGLTDDTIISASFSPIQRESGFVSGLVCVLHDITDQVQEEEERKQFVSNVSHELRTPLTSVRSYVEALSDGAINDPELSPRFLKVVEDETERMIRMINDLLSLSRMDSGTTKLDLEYVNIGELFNYILNRFDMIIKNETADDSDQKKYTIDRHITNKSLWVEIDPDKFTQVIDNIMNNAVKYSPDGGVITARLTDTHNHVILSITDQGLGIPRKDLRHVFDRFFRVDKARSRKQGGTGLGLAISKEVINLLGGQIWVNSTEGKGSTFYIALPYVPFEEDDWDDQVDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL43</accession>
    <name>H4GL43_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribonuclease J</fullName>
            <shortName evidence="9">RNase J</shortName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rnj</name>
        <name evidence="12" type="ORF">PS3_6966</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">An RNase that has 5'-3' exonuclease and possibly endonuclease activity. Involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer, may be a subunit of the RNA degradosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Bacterial RNase J subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122775.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6966"/>
    <dbReference type="EnsemblBacteria" id="EHS84673">
        <property type="protein sequence ID" value="EHS84673"/>
        <property type="gene ID" value="PS3_6966"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1798"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' exoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01491">
        <property type="entry name" value="RNase_J_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004613">
        <property type="entry name" value="RNase_J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042173">
        <property type="entry name" value="RNase_J_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030854">
        <property type="entry name" value="RNase_J_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041636">
        <property type="entry name" value="RNase_J_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17770">
        <property type="entry name" value="RNase_J_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004803">
        <property type="entry name" value="RnjA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00649">
        <property type="entry name" value="MG423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lactamase_B" evidence="11">
        <location>
            <begin position="16"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="552"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="554"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS84673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="592" mass="65965" checksum="625816CCB9E99EF6" modified="2012-04-18" version="1">MSNIKMIPFGGVRENGKNMYAVEVNDKIFILDCGLKYPENELMGIDFVIPDWSYLDDLADKIVGVFLTQGHADAIGALPYFLAEFDVPAFGSELSIALAKLAVKGNKESKKFNDFHVVDENTEIDFDDVTVSFFPTTHSIPDTLGIVLATPDGQIVYTGDFKFDQAATKGYQTDLARLAQIGQEGVLLLLSDSAGSEITGASSRETDIRQYIEDTFENSMSRIIVASVASNILRVQQILDAAHNTNRKVVLSGSDIEQIVQTALDLELLTLPTDDILIKLDEANQLPDDEVVILVTGRMGEPIKSLLAIANDEDPDIKFKEGDLVFVTTTPSHAVETVVQKAKDAIYKADAEVKFIADDLNPSGHASQNDEQLMLNLLHPKYFVPIQGEYRMLHQHEVLAEEVGVDPDNIFIMSRGDVLTYTDGDFHLGEKVNAGNVMIDGTGVGDIGNIVLRDRRILSEDGIFVVVATIDRKKKKIVARPQITSRGFVFVKTNRQLMKQSADLVEEVVQENLNQKDFDWGTLKQEVRDKLNRFLFNQTKRHPVILPVIMEINQHPKRKNRSNKSADEPTANDQTKEKKHRGRGRGKKKNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL60</accession>
    <name>H4GL60_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribose-phosphate pyrophosphokinase</fullName>
            <shortName evidence="6">RPPK</shortName>
            <ecNumber evidence="6">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">5-phospho-D-ribosyl alpha-1-diphosphate</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosyl pyrophosphate synthase</fullName>
            <shortName evidence="6">P-Rib-PP synthase</shortName>
            <shortName evidence="6">PRPP synthase</shortName>
            <shortName evidence="6">PRPPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">prs</name>
        <name evidence="8" type="ORF">PS3_19311</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ribose-phosphate pyrophosphokinase family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122792.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19311"/>
    <dbReference type="EnsemblBacteria" id="EHS84690">
        <property type="protein sequence ID" value="EHS84690"/>
        <property type="gene ID" value="PS3_19311"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1819"/>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009165">
        <property type="term" value="P:nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00583_B">
        <property type="entry name" value="RibP_PPkinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037515">
        <property type="entry name" value="Rib-P_diPkinase_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="PTHR10210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran_N" evidence="7">
        <location>
            <begin position="11"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="43"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="102"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="325" mass="35864" checksum="61E3483D39631DE7" modified="2012-04-18" version="1">MSELQNAADVRLFALNSNRPLAEKIAEELGLPLSECTVSHFADGEISITINESVRGREVYVIQSTSDPVNDNLMELLIMIDALRRASAKKINVVLPYYGYARQDRKARSREPITAKLVANLLELDQIDRLIAVDLHAAQVQGFFDIPVDHLQAAPTLAKYFLDQAISDDLVIVSPDHAGMTMARRFAELLGDDVSVAIVDKRTEDTRENPDLTMPSMIIGDVKDKVAIIVDDIIDTGNRLTNSAELVSKKGAKEVFAVATHPVLSGDASERIQASAIKEMVTTDTIVVPETKQFDELKQLSVAPLLAEAIIRIHNHQSIHHLFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GLB1</accession>
    <name>H4GLB1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA repair protein RadC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_12671</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0758 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000080">
        <property type="protein sequence ID" value="EHS84610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122843.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000080.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12671"/>
    <dbReference type="EnsemblBacteria" id="EHS84610">
        <property type="protein sequence ID" value="EHS84610"/>
        <property type="gene ID" value="PS3_12671"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1889"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08071">
        <property type="entry name" value="MPN_DUF2466"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025657">
        <property type="entry name" value="RadC_JAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001405">
        <property type="entry name" value="UPF0758"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020891">
        <property type="entry name" value="UPF0758_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30471">
        <property type="entry name" value="PTHR30471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04002">
        <property type="entry name" value="RadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01302">
        <property type="entry name" value="UPF0758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="7">
        <location>
            <begin position="79"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS84610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="200" mass="23083" checksum="6723B2C69CF85E7C" modified="2012-04-18" version="1">MRTYLSTNNFEELVQMMLPDRRLAQRFIATFGSFQELHHAQREQMQSLIDYDPVNFRSVIAAIQIGQQALTRPQPMTGQLNSSYELGQQLIHELAGEDQEQVMIIGVDVHSVVIDRQVLFKGGSHECQIYMDQVFRYLIQIGAHGLIFAHNHPSGLVEPSPEDRQFFKQLQASCRLMNIRLVDCMIVGRNDYFSWQEKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHR4</accession>
    <name>H4GHR4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Homocysteine S-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_13004</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000004">
        <property type="protein sequence ID" value="EHS87544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121598.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000004.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13004"/>
    <dbReference type="EnsemblBacteria" id="EHS87544">
        <property type="protein sequence ID" value="EHS87544"/>
        <property type="gene ID" value="PS3_13004"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.26"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047150">
        <property type="term" value="F:betaine-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017226">
        <property type="entry name" value="Betaine-hCys_S-MeTrfase_BHMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003726">
        <property type="entry name" value="HCY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036589">
        <property type="entry name" value="HCY_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02574">
        <property type="entry name" value="S-methyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037505">
        <property type="entry name" value="Betaine_HMT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82282">
        <property type="entry name" value="SSF82282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50970">
        <property type="entry name" value="HCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Hcy-binding" evidence="4">
        <location>
            <begin position="1"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="307" mass="33089" checksum="56620001A821F489" modified="2012-04-18" version="1">MELAELLKETPIVIDGSMSTPLEIWGAQTDSDLWTAAALINHPDLVKRVHQAYFEAGARITITDSYQTNVAAFEKHGYGEQAARRLIRLSAQLAQTARDEYEKATGVHNLVAGSIGPYGAYLADGSEYRGDYELSLADYQDFHAPRLEELLAAGVDCLAIETQPKLAEVTAILAWLHDHQISVPVWVSFSLQDPQTISEGTALTQAVEAIQDDLNVLAVGVNCMPVTMATPAVETIAKVASVPIIVYPNSGAQYDPITKTWQTTTGQTSFAQAAVDWVQAGAGIIGGCCTTMPKDIQEIKLAIAKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHS9</accession>
    <name>H4GHS9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0346 protein PS3_10989</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_10989</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0346 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121613.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10989"/>
    <dbReference type="EnsemblBacteria" id="EHS87485">
        <property type="protein sequence ID" value="EHS87485"/>
        <property type="gene ID" value="PS3_10989"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.48"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01538">
        <property type="entry name" value="UPF0346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010673">
        <property type="entry name" value="UPF0346"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023089">
        <property type="entry name" value="YozE_SAM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036806">
        <property type="entry name" value="YozE_SAM-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06855">
        <property type="entry name" value="YozE_SAM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037262">
        <property type="entry name" value="UCP037262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140652">
        <property type="entry name" value="SSF140652"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YozE_SAM_like" evidence="2">
        <location>
            <begin position="5"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="74" mass="9019" checksum="9773FFA679F359BC" modified="2012-04-18" version="1">MYRESFYQFLMTQRNPNQPNEIEQFANDAFFDSAFPKRSQDFDEISKYLEENATYLYSMTIFDDAWQLYLAKMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHX1</accession>
    <name>H4GHX1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glycine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="8">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glycyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="8">GlyRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">glyQ</name>
        <name evidence="9" type="ORF">PS3_12183</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121655.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12183"/>
    <dbReference type="EnsemblBacteria" id="EHS87527">
        <property type="protein sequence ID" value="EHS87527"/>
        <property type="gene ID" value="PS3_12183"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.97"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00733">
        <property type="entry name" value="GlyRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00254">
        <property type="entry name" value="Gly_tRNA_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006194">
        <property type="entry name" value="Gly-tRNA-synth_heterodimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002310">
        <property type="entry name" value="Gly-tRNA_ligase_asu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02091">
        <property type="entry name" value="tRNA-synt_2e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01044">
        <property type="entry name" value="TRNASYNTHGA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00388">
        <property type="entry name" value="glyQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50861">
        <property type="entry name" value="AA_TRNA_LIGASE_II_GLYAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="321" mass="37068" checksum="94AD64CB18F7BE72" modified="2012-04-18" version="1">MDQKLTVQDIIFKLQQYWGSQGCMLMQAYDNEKGAGTMSPYTFLRAIGPEPWNAAYVEPSRRPADGRYGDNPNRLYQHHQFQVLMKPSPENIQELYLGSLRELGIEPLEHDIRFVEDNWENPSMGCAGVGWEIWLDGMEVTQFTYFQVVGELQMDPVAAEITYGLERLAEYIQNVDSVYDLEWADGVYYGDIFKEPEYEHSKYSFEESNQDMLLKFFEDYEAEAKRLIALGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYLHRIRIMAKSVAKAFVQERRKQGFPLIHDEAKRQAVLAKYQAQAEQEAKRNAKKGDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHX3</accession>
    <name>H4GHX3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">GTPase Era</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">era</name>
        <name evidence="12" type="ORF">PS3_9707</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 8 9">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Era GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121657.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9707"/>
    <dbReference type="EnsemblBacteria" id="EHS87529">
        <property type="protein sequence ID" value="EHS87529"/>
        <property type="gene ID" value="PS3_9707"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.99"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04163">
        <property type="entry name" value="Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00367">
        <property type="entry name" value="GTPase_Era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030388">
        <property type="entry name" value="G_ERA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005662">
        <property type="entry name" value="GTP-bd_Era"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42698">
        <property type="entry name" value="PTHR42698"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00436">
        <property type="entry name" value="era"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51713">
        <property type="entry name" value="G_ERA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Era-type G" evidence="11">
        <location>
            <begin position="9"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="KH type-2" evidence="10">
        <location>
            <begin position="207"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="8">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="8">
        <location>
            <begin position="43"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="8">
        <location>
            <begin position="64"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="8">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="8">
        <location>
            <begin position="155"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="64"/>
            <end position="68"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="303" mass="34017" checksum="4E7890FDF7350795" modified="2012-04-18" version="1">MDTLKADFHSGFVAIIGRPNVGKSTLLNYVVGQKVAIMSNVAQTTRNKIQGIYTTNEAQIIFIDTPGIHKPQTKLGDFMERSALSALDEVDAILFVVSATEKRGPGDNFIIERLKKVQQPIYLVINKIDQVHPNDLPEIVAQYNDTLPFKKVIPVSALQGNNVNGLIDELVDNLPVGPQYYPTDQVSDHPERFVVAEMIREKVFLLTRQEVPHSVAIDVTSIKRNNEETIEVDANIIVERPGQKGIIIGKKGSMLKEIGTQARQDIERLLGDKVYLHLWVKVVPGWRDKNAMLKDYGYRSTDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GHY2</accession>
    <name>H4GHY2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D-aminoacyl-tRNA deacylase</fullName>
            <shortName evidence="1">DTD</shortName>
            <ecNumber evidence="1">3.1.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Gly-tRNA(Ala) deacylase</fullName>
            <ecNumber evidence="1">3.1.1.-</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dtd</name>
        <name evidence="2" type="ORF">PS3_14587</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a D-aminoacyl-tRNA + H2O = a D-alpha-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glycyl-tRNA(Ala) + H2O = glycine + H(+) + tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DTD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="1"/>
    <dbReference type="EC" id="3.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AICN01000005">
        <property type="protein sequence ID" value="EHS87538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121666.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000005.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14587"/>
    <dbReference type="EnsemblBacteria" id="EHS87538">
        <property type="protein sequence ID" value="EHS87538"/>
        <property type="gene ID" value="PS3_14587"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.109"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051499">
        <property type="term" value="F:D-aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106026">
        <property type="term" value="F:Gly-tRNA(Ala) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043908">
        <property type="term" value="F:Ser(Gly)-tRNA(Ala) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00563">
        <property type="entry name" value="Dtyr_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00518">
        <property type="entry name" value="Deacylase_Dtd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003732">
        <property type="entry name" value="Daa-tRNA_deacyls_DTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10472">
        <property type="entry name" value="PTHR10472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02580">
        <property type="entry name" value="Tyr_Deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69500">
        <property type="entry name" value="SSF69500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00256">
        <property type="entry name" value="TIGR00256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0820">tRNA-binding</keyword>
    <feature type="short sequence motif" description="Gly-cisPro motif, important for rejection of L-amino acids" evidence="1">
        <location>
            <begin position="137"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="145" mass="16000" checksum="B8B8981543D02F2E" modified="2012-04-18" version="1">MKVVLQRVKQAQVTVDEEVIGEIEQGFLLLVGFGPDDNDETLDYLVHKITNLRVFEDEAGKLNLSLKDVQGSILSVSQFTLYANTKKGNRPSFTEAAQPEQATQLYDDFNHRLAATGIPVATGKFGANMQVSLINDGPVTIIYEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI05</accession>
    <name>H4GI05_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">Peptidyl-tRNA hydrolase</fullName>
            <shortName evidence="1">PTH</shortName>
            <ecNumber evidence="1 2">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pth</name>
        <name evidence="4" type="ORF">PS3_7501</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = a tRNA + an N-acyl-L-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the PTH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1 2"/>
    <dbReference type="EMBL" id="AICN01000007">
        <property type="protein sequence ID" value="EHS87456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121689.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000007.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7501"/>
    <dbReference type="EnsemblBacteria" id="EHS87456">
        <property type="protein sequence ID" value="EHS87456"/>
        <property type="gene ID" value="PS3_7501"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.138"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:aminoacyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00462">
        <property type="entry name" value="PTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00083">
        <property type="entry name" value="Pept_tRNA_hydro_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001328">
        <property type="entry name" value="Pept_tRNA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018171">
        <property type="entry name" value="Pept_tRNA_hydro_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036416">
        <property type="entry name" value="Pept_tRNA_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224">
        <property type="entry name" value="PTHR17224"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01195">
        <property type="entry name" value="Pept_tRNA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53178">
        <property type="entry name" value="SSF53178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00447">
        <property type="entry name" value="pth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01195">
        <property type="entry name" value="PEPT_TRNA_HYDROL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01196">
        <property type="entry name" value="PEPT_TRNA_HYDROL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="186" mass="20506" checksum="EF77A3EC4271CAD7" modified="2012-04-18" version="1">MKMIVGLGNIGSQYDETRHNCGFMVVDQMARDYHLGAFKHEKAPAVTVTGMINGHKVMLVKPTTFMNDSGHAVKPLMDYYDLDLADLVIVNDDLDMPVGQVRLKAKGSAGGHNGLRSIIDHLGTQQFNRVKVGIDHPQQGTVVSHVLGKFTTTERATIDDSIEVAEHALVDWIDGATFKELMNNYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI10</accession>
    <name>H4GI10_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DEAD-box ATP-dependent RNA helicase CshA</fullName>
            <ecNumber evidence="6">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cshA</name>
        <name evidence="13" type="ORF">PS3_3364</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DEAD-box RNA helicase possibly involved in RNA degradation. Unwinds dsRNA in both 5'- and 3'-directions, has RNA-dependent ATPase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Oligomerizes, may be a member of the RNA degradosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family. CshA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000007">
        <property type="protein sequence ID" value="EHS87461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121694.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000007.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3364"/>
    <dbReference type="EnsemblBacteria" id="EHS87461">
        <property type="protein sequence ID" value="EHS87461"/>
        <property type="gene ID" value="PS3_3364"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.144"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010501">
        <property type="term" value="P:RNA secondary structure unwinding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01493">
        <property type="entry name" value="DEAD_helicase_CshA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030880">
        <property type="entry name" value="DEAD_helicase_CshA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Q_MOTIF" evidence="12">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="32"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="213"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="428"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="428"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="459"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS87461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="498" mass="56184" checksum="71E9386749E4C714" modified="2012-04-18" version="1">MYFSELGLSNKLLKAVQRSGYEEATPIQEQTIPMVLKGLDVIGQAQTGTGKTAAFGLPVLEHVDTQSSAIQALIISPTRELAIQTQEELYKLGKEKRARVQVVYGGADIRRQIKALKNGPQIIVGTPGRLRDHLNRGTIKLDQLQTLVLDEADEMLNMGFLDDIEAIIQATPSDRQTLLFSATMPPEIKRIGVQFMKDPQTVKIKAKELTTDLVDQYYIKARDYEKFDIMTRLIDVQDPDLTIVFGRTKRRVDELSRGLIARGYNAAGIHGDLTQDRRSKIMKRFKNGELDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAGNHGVSLTFVTPNEMDYLHEIEKLTKVRMLPLKPPTAEEAFRGQVASAFNDINELIERPDNDRYREAASELLATHEAVDLVATLLNELTKDDETQVPVKITPERPLPNRKRKPNRNGGNHYRRRSNGGNRRGDNRNRRPRGGNGSHRNPRSQGKRNSFKIRRKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI26</accession>
    <name>H4GI26_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Two-component system response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_3349</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000011">
        <property type="protein sequence ID" value="EHS87436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3349"/>
    <dbReference type="EnsemblBacteria" id="EHS87436">
        <property type="protein sequence ID" value="EHS87436"/>
        <property type="gene ID" value="PS3_3349"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.165"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="7">
        <location>
            <begin position="135"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="135"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="233" mass="26683" checksum="2A3ADA05E671C188" modified="2012-04-18" version="1">MVSQDPQLQETMQELASINHWQIDQTTTATSAVAKIAQASYAALLWDLDTINLETTLATMMLLRPEFHHPILVWVQAPTSRQSRKLFRAKVDDVLVKTTPLEVLSALIQQRLWLYDQNTLEPDPINNPPESPVNQHQIQIANWIIDKQKVSVAKDHQPVLLTKKEFQLLSYLADHQGQVLSRDQLLSGVWGYDELGSSRIVDMHIAHLRDKLEDDDQNPQHLLTVRGFGYKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI48</accession>
    <name>H4GI48_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7 8">Transcription elongation factor GreA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Transcript cleavage factor GreA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">greA</name>
        <name evidence="11" type="ORF">PS3_9430</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7 8">Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the GreA/GreB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121732.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9430"/>
    <dbReference type="EnsemblBacteria" id="EHS87387">
        <property type="protein sequence ID" value="EHS87387"/>
        <property type="gene ID" value="PS3_9430"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.193"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00105">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036953">
        <property type="entry name" value="GreA/GreB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018151">
        <property type="entry name" value="TF_GreA/GreB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006359">
        <property type="entry name" value="Tscrpt_elong_fac_GreA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028624">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001437">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023459">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022691">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036805">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30437">
        <property type="entry name" value="PTHR30437"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01272">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03449">
        <property type="entry name" value="GreA_GreB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006092">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46557">
        <property type="entry name" value="SSF46557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01462">
        <property type="entry name" value="greA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00829">
        <property type="entry name" value="GREAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00830">
        <property type="entry name" value="GREAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="GreA_GreB_N" evidence="10">
        <location>
            <begin position="8"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="GreA_GreB" evidence="9">
        <location>
            <begin position="85"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="159" mass="17841" checksum="94887F2BCD86F315" modified="2012-04-18" version="1">MADEKTYPMTLEGKAKLEEELENFRLVRRPEVINRIKIARSYGDLSENSEYESAKDEQSFVEGRIAQIENMLQYAEIIDNDNVDADEVALGREVTFKELPDEEPESYVIVGESESDPMSGKISNESPMAKSLIGHKIGDKVNVELPNGGSMEVEVLDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI52</accession>
    <name>H4GI52_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HD superfamily metal-dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_14501</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14501"/>
    <dbReference type="EnsemblBacteria" id="EHS87391">
        <property type="protein sequence ID" value="EHS87391"/>
        <property type="gene ID" value="PS3_14501"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.199"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="2">
        <location>
            <begin position="49"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="200" mass="23885" checksum="FBEA6F6B17ED9614" modified="2012-04-18" version="1">MDLERTRPIMKTDQSWRKDPEYVAIVEDLLAKPEVKALANYTQHHHSNRLQHSIAVSYDSYRVAKKLNLDYESTARAGILHDLFYYDWRTTKFDLGTHAFIHPRVALRNAEKLTDLNDKEQDIILKHMFGSTLAVPKYWESLIVSLVDDYEAEHEFFRPMRKKLRHQKRNLQRRYQASRRQTRHQMRGERVNGTVINNHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GI84</accession>
    <name>H4GI84_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Cysteine synthase</fullName>
            <ecNumber evidence="5 11">2.5.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PS3_13028</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>hydrogen sulfide + O-acetyl-L-serine = acetate + L-cysteine</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="5 11"/>
    <dbReference type="EMBL" id="AICN01000013">
        <property type="protein sequence ID" value="EHS87423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121768.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000013.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13028"/>
    <dbReference type="EnsemblBacteria" id="EHS87423">
        <property type="protein sequence ID" value="EHS87423"/>
        <property type="gene ID" value="PS3_13028"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.241"/>
    <dbReference type="UniPathway" id="UPA00136">
        <property type="reaction ID" value="UER00200"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005856">
        <property type="entry name" value="Cys_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005859">
        <property type="entry name" value="CysK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01139">
        <property type="entry name" value="cysK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01136">
        <property type="entry name" value="cysKM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="7 9 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PALP" evidence="12">
        <location>
            <begin position="10"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="9">
        <location>
            <begin position="181"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605856-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS87423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="310" mass="32414" checksum="C81D4EA17451AB31" modified="2012-04-18" version="1">MTKQIYNSINELIGNTPIVKLNRVVPEGAADVYVKLEFFNPAGSIKDRIALAMIEKAEADGLLKAGDTIVEPTSGNTGIGLAAVAAAKGYHLVITMPETMSVERRKLMQGYGAELILTPGADGMKGSIAKAQELVDQKGYFMPMQFENPANPAAHEATTGKEIIAAFGEDDLPDAFVAGVGTGGTLTGIGHDLKAVNDQVKVYALEPAESPLLKDGQAGKHKIQGISAGFIPATLDQDVYDGIVEISSDEAIATAQAVSRQEGFLPGISAGANIAGAIQLAQELGVGKKVITVAPDGGERYLSTDLFNFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIA2</accession>
    <name>H4GIA2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_14222</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121786.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14222"/>
    <dbReference type="EnsemblBacteria" id="EHS87323">
        <property type="protein sequence ID" value="EHS87323"/>
        <property type="gene ID" value="PS3_14222"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.267"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="217" mass="24595" checksum="3E4CBF472C6B5346" modified="2012-04-18" version="1">MNPSYQLLLILISALEITFTKKLLPNLIIIIITILYLMYKNLPARTWWWILAAPIIPGLAILVTIGWFGSHNIMYAWILVSRIYVYILTGTAIVKTHSTLTLTRSLEQNLHLPPKYAYGVLAALNLISKTIENVQKIKQAAAMRGVILNYWSPQLYFKAILTAIHWSDELAQAMESHGFNEVQSRTVSHAIAVKTSDWWLLIGGLIILQLLLIFCPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GID9</accession>
    <name>H4GID9_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein UspA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_4375</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS87360">
        <property type="protein sequence ID" value="EHS87360"/>
        <property type="gene ID" value="PS3_4375"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.333"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="9"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="156" mass="17630" checksum="3BA896987425EBBA" modified="2012-04-18" version="1">MEVEIMQEYNNILVPVDGSKEAEIAFEKAVNNAKRNHAHLDVLNVIDTRAMSYNFAGMSDSSIAYQLVDKSQEYLNELVQRAKDEFDFTDVDVHIRLGNPKTVIAYDFPKDHHNDLIMIGASGLTRIQRAMIGSVTSYVKRCAPIDVLVVRTELDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIE0</accession>
    <name>H4GIE0_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_8377</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121824.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8377"/>
    <dbReference type="EnsemblBacteria" id="EHS87361">
        <property type="protein sequence ID" value="EHS87361"/>
        <property type="gene ID" value="PS3_8377"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.334"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS87361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="153" mass="17336" checksum="EA5A53C50EEFD2D6" modified="2012-04-18" version="1">MDAIDKKILNMLQENARISLKELSNACFISSPAIAARINKLEKSGIINGYHMSINLDAIDYYVKAFIMVQLEPRQKDEFYPYVQSVPNVIECNCVTGDFSEVMEVAFPTTAELDSFINEIQRRFGKTSTQIVFSTSVENRGIMFSDAEEEESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIE3</accession>
    <name>H4GIE3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
            <shortName evidence="5">DHBP synthase</shortName>
            <ecNumber evidence="5">4.1.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PS3_4584</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="5">Binds 2 divalent metal cations per subunit. Magnesium or manganese.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DHBP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.12" evidence="5"/>
    <dbReference type="EMBL" id="AICN01000014">
        <property type="protein sequence ID" value="EHS87364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121827.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000014.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4584"/>
    <dbReference type="EnsemblBacteria" id="EHS87364">
        <property type="protein sequence ID" value="EHS87364"/>
        <property type="gene ID" value="PS3_4584"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.338"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0686">Riboflavin biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="219" mass="23607" checksum="26D76A1634824C83" modified="2012-04-18" version="1">MEFSTVEVALEHLQKGGFIILADDEGRENEGDLVALAQDIQPAVVYQMLHEANGLMCVPMPKALTEKLGFTPMVKHSTDPHGTPFTITADGTLEATGVTTGVSAFDRAATIQQMAKPDAKATDFNHPGHIQPLYAQPNGLRDRVGHTEAAVDLAYLAHRQPVAVIIEVLKADGTMARRDALAGMADRLEVPFLTIDAIVKYMDDHGIVKAADEVAELVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIG0</accession>
    <name>H4GIG0_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Glucose-6-phosphate isomerase</fullName>
            <ecNumber evidence="3 8">5.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PS3_6975</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the GPI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="3 8"/>
    <dbReference type="EMBL" id="AICN01000016">
        <property type="protein sequence ID" value="EHS87303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6975"/>
    <dbReference type="EnsemblBacteria" id="EHS87303">
        <property type="protein sequence ID" value="EHS87303"/>
        <property type="gene ID" value="PS3_6975"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.357"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="PTHR11469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="5 8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 8 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="417" mass="45820" checksum="39E0047BA4FA6B38" modified="2012-04-18" version="1">MRNGTGAGADFRDWLHLPTEYDQEEFARIKAAAKKIQADSDFLVTIGIGGSYLGARMAIDFLHNSFYQAQTPDARKAPFVVFAGNSLSSSYLQDLITLIGDHDFSINVISKSGTTTEPSIAFRVFHELLVKKYGEEAANQRIYATTDRARGALKTEADANGWETFVIPDGVGGRYSVLTAVGLLPIAASGADIDQLMAGAAEAEKTYVDPDLSKNEAYQYAAYRNILYRKGYTTELLENYEPNMAMFAEWWKQLAGESEGKDQKGIYPSSANFTTDLHSLGQYIQEGLRNLMETVVKLDTPNHDLDVPGAANDLDGLGYLEGKSIDWVNTQAYKAVVAAHNAGGVPVMTVHIDEENEYTLGYMIYFFEVAMGISGYLNGINPFNQPGVEAYKTNMFGLLGKPGFEEVGDQLRAEMDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIH1</accession>
    <name>H4GIH1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA modification GTPase MnmE</fullName>
            <ecNumber evidence="6">3.6.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mnmE</name>
        <name evidence="6" type="synonym">trmE</name>
        <name evidence="9" type="ORF">PS3_8611</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="6">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer. Heterotetramer of two MnmE and two MnmG subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.-.-" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000017">
        <property type="protein sequence ID" value="EHS87292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121855.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000017.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8611"/>
    <dbReference type="EnsemblBacteria" id="EHS87292">
        <property type="protein sequence ID" value="EHS87292"/>
        <property type="gene ID" value="PS3_8611"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.372"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04164">
        <property type="entry name" value="trmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00379">
        <property type="entry name" value="GTPase_MnmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031168">
        <property type="entry name" value="G_TrmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018948">
        <property type="entry name" value="GTP-bd_TrmE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004520">
        <property type="entry name" value="GTPase_MnmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027368">
        <property type="entry name" value="MnmE_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025867">
        <property type="entry name" value="MnmE_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT_dom1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12631">
        <property type="entry name" value="MnmE_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10396">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00450">
        <property type="entry name" value="mnmE_trmE_thdF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51709">
        <property type="entry name" value="G_TRME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0630">Potassium</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TrmE-type G" evidence="8">
        <location>
            <begin position="223"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="233"/>
            <end position="238"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="252"/>
            <end position="258"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="277"/>
            <end position="280"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="462"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS87292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="462" mass="50946" checksum="660A46224FD23895" modified="2012-04-18" version="1">MVKTDQDTIAAISTPVGEGGISIIRVSGDDALPVVQKLFRGKDLAKVATHTINYGHIVDPSNQQEVDEVMVSVMRAPRTYTREDVIEINCHGGLLATNRILQLVLSYGARLADPGEFTQRAFINGRLDLSQAEAVMDLIRAKTDRSMDVALHQLDGDLSRLVRQLRQDILDVLAQVEVNIDYPEYDAVEEMTSQMLREKATMVKERVSGLLKTAQQGKVLRDGLATAIIGRPNVGKSSLLNALLHEDKAIVTNVAGTTRDVIEEYVNVEGVPLKLIDTAGIRHTDDQVEKIGVERSRQAITTADLILLLIDQSRPLSEEDRELITATEQTPRMVILNKTDLPSVIELNDLKTLVNGDPIIQTSILEQSGMQELGQAISHRFFNEGIENRQNQVMVTNARHIGLLKQVESALTEVLSGLDAGLPVDLVQIDMTTAWNLLGEITGDSYQDELLTELFSQFCLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>H4GII4</accession>
    <name>H4GII4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metal-dependent membrane protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_3222</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0177 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000018">
        <property type="protein sequence ID" value="EHS87279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121868.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000018.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3222"/>
    <dbReference type="EnsemblBacteria" id="EHS87279">
        <property type="protein sequence ID" value="EHS87279"/>
        <property type="gene ID" value="PS3_3222"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.386"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="237" mass="26817" checksum="E43BC84A4BE48DDC" modified="2012-04-18" version="1">MNSSNKQLSLSQRILIMIALALVIQLPPVIELMIGSGLHNLNYHWMPWIVTFGVMAYTILFFVIILWAYQLFKRYRRWPVRTKSWGSQIGWILISYLVLLSGVGALSTLNQLLYHQTTTSNNDTIQSLAQSSPVLLVITALSGIFLSPLAEELIFRGVLMNFFFKASSFWPPILLSGLIFTLEHSSTTIVSYLIYFFMGGVLAFVYRATGQLKISIGLHFLNNLIAMLELIIPILIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIJ5</accession>
    <name>H4GIJ5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_5720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000020">
        <property type="protein sequence ID" value="EHS87274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121879.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000020.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5720"/>
    <dbReference type="EnsemblBacteria" id="EHS87274">
        <property type="protein sequence ID" value="EHS87274"/>
        <property type="gene ID" value="PS3_5720"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.403"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="46"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="153"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="342" mass="39720" checksum="A8F2EFAA2789B6F4" modified="2012-04-18" version="1">MIKITMYSQADSVAGQGVGSAYIELMGLLKDRFKDQFDIHVNQYGESDISHYHTINPTYFLSTFSKKRGRKIGYVHFLPETLEGSIKLPKVFRKIFYWYVIAFYRRMDELVVVNPSFIDKLVAYQIPRDRITYIPNFVDDQHFYPMNADDRLALRQKYHLKSNQFIVMGSGQIQERKGVFDFIQLAKDNPQVQFIWAGGFSFGRITDGYQELKKVVDNPPANLWFPGIVSRSEINELLNLADLFLLPSYNELFPMSVLEAFSTGTAVMLRDLDLYHDIMDGYYLPSHDEKDMNLKLQQLIHDPTKLKELQQVALTASQRYSKDHLAGIWQDFYIDQAHKGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIM6</accession>
    <name>H4GIM6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA-directed DNA polymerase III delta prime subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_11357</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121910.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000021.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11357"/>
    <dbReference type="EnsemblBacteria" id="EHS87221">
        <property type="protein sequence ID" value="EHS87221"/>
        <property type="gene ID" value="PS3_11357"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.440"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004622">
        <property type="entry name" value="DNA_pol_HolB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00678">
        <property type="entry name" value="holB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 4" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="339" mass="38594" checksum="5BBBD17DA779E939" modified="2012-04-18" version="1">MTESLLTANDRQPQLVKRLQGLIDHNELVHAYLLVGPSGSGKKLLAQWVALRLFCLSPVNSQPDLTCDECQRILSNNHPDVVVAGPDGRQIKVDQIRHLKDEFSKSSVEGRQKVFIIEDAEKMTVNAANSLLKFIEEPGTDIYIFLLTTNKNAILPTIQSRTQIIELQPVSDEVGRELMTQAQIPDYLQPVIWGLTKSVDQAQSLLENDWLGQVVKLLVPWVQKLAGEDWLAFVEVQTKWMPLAIDRAHQQVIFDLIMLVVRDLLFIKQGVATNLIHFQQWYQPLSEVVSRLSSEQILFISERTLGLRHQLDQNINFQNILEQFVLQCFTRLGGYHRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIN6</accession>
    <name>H4GIN6_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha-1,4 glucan phosphorylase</fullName>
            <ecNumber evidence="6">2.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_21084</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.</text>
    </comment>
    <comment type="function">
        <text evidence="4">Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + phosphate = [(1-&gt;4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:41732"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9586"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000021">
        <property type="protein sequence ID" value="EHS87231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21084"/>
    <dbReference type="EnsemblBacteria" id="EHS87231">
        <property type="protein sequence ID" value="EHS87231"/>
        <property type="gene ID" value="PS3_21084"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.452"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102250">
        <property type="term" value="F:linear malto-oligosaccharide phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102499">
        <property type="term" value="F:SHG alpha-glucan phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011833">
        <property type="entry name" value="Glycg_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468">
        <property type="entry name" value="PTHR11468"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000460">
        <property type="entry name" value="Pprylas_GlgP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02093">
        <property type="entry name" value="P_ylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000460-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS87231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="799" mass="91049" checksum="1D39894E55F47562" modified="2012-04-18" version="1">MMMTKDEFKQAFLDELDTHFSVAIDEATDYELFEALSAVVRHGYSENWRRTRTEDNRQQTKQVYYFSIEFLPGTLLRSNLLNLGWLDQAREALHDLKLDLDQVALAEPDLALGNGGLGRLASCFMDSLASEGYAANGNGIYYKYGLFKQKSVDGYQVEMPNDWTKSDSPWLVRRKGKAALVHFGGHYTMIEQDGWLTPEYEGDQVVLAVPYDIAVVGYHNHVTNTMRLWNAEIPSEDEIDYRTIQDRRTIEDLTSVLYPNDSTYDGRLLRLKQEYFFVSAGIQSILRYFVKNNGTDAWDQLPDEVAIHINDTHPAMCVAELMRLLVDDYRVDWERAWQITQQVMSYTNHTIMAEALEKWDVNMFRQLLPRLMDIITEIDRRYVESLQGRVDGQIIDRTRIISGNQVHMAHLAIIGSHSINGVAELHTQLLKTTVLNDFYNLYPDRFNNKTNGITLRRWLQLANPQLSHLLDEQLGSQWRNDSAQLLKLTKEADDPKVLNQLAKIKLANKQKLAQLIKQQTGLEVDPQAIFDVQVKRLHAYKRQTLKLLHVLALYRDLKNGIDHPKRVVIFGAKAAPSYVYAKQVIKVINEVAKLINHDPDINDRLKVVFLENYGVTLAEQIIPAADLSEQISTTTKEASGTSNMKLMANGALTIATMDGANVEIAEAVGDDNIFIFGLNKDQVNQAYADHSYDPQQVYNQSPVVKSVVDMLIDGTIPACQVEGKALYDSFVQANEPFFVLADLESYLQAQSRVETAWQDQEAWAKRSLINIAHAERFDADHTVQLYAQDIWHLTPLAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIS3</accession>
    <name>H4GIS3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glycerol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_21278</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000022">
        <property type="protein sequence ID" value="EHS87189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121957.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000022.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_21278"/>
    <dbReference type="EnsemblBacteria" id="EHS87189">
        <property type="protein sequence ID" value="EHS87189"/>
        <property type="gene ID" value="PS3_21278"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.499"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018211">
        <property type="entry name" value="ADH_Fe_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016205">
        <property type="entry name" value="Glycerol_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616">
        <property type="entry name" value="PTHR43616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000112">
        <property type="entry name" value="Glycerol_dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00913">
        <property type="entry name" value="ADH_IRON_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Fe-ADH" evidence="7">
        <location>
            <begin position="8"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="94"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="116"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="363" mass="38980" checksum="7C2B956F97BDC795" modified="2012-04-18" version="1">MVIGFASPSTYIQGKNVILDSDKYLQAFGKHPLLLAGSTAYRIIGQDFEEYLQAHDYTVTYAPFNGESSDQEVDRVAKIGHDHQVSVVYGLGGGKTVDTAKAVADKLDLPVVIIPTLASNDAPCSRLSVIYTEDGNFDHYLFYKKNPDLVLVDTQVIANGPVRMLISGIADALATNVEAEAVAQANADTMLDQKQSLVGSAIGHQCEATLFNYSHEAIAAAESHAVTKALENIIEANTLMSGLGFESGGLSGAHSIHDGLTTLSQTHELTHGEKVAYGTLTQLMLEGADQVRYEKYYRFIKSLGLPTTLADLNLSNVTDEELLEAGKAACSENDTMDRLPFEVTPSDVVDALKAVDAYSKANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIU7</accession>
    <name>H4GIU7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">50S ribosomal protein L24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rplX</name>
        <name evidence="6" type="ORF">PS3_7754</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">One of the proteins that surrounds the polypeptide exit tunnel on the outside of the subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="3">One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121980.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7754"/>
    <dbReference type="EnsemblBacteria" id="EHS87177">
        <property type="protein sequence ID" value="EHS87177"/>
        <property type="gene ID" value="PS3_7754"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.529"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_B">
        <property type="entry name" value="Ribosomal_L24_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="KOW_RPL26/RPL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003256">
        <property type="entry name" value="Ribosomal_L24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_L24/26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12903">
        <property type="entry name" value="PTHR12903"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17136">
        <property type="entry name" value="ribosomal_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01079">
        <property type="entry name" value="rplX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="KOW" evidence="5">
        <location>
            <begin position="2"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="102" mass="11008" checksum="46687015826DB591" modified="2012-04-18" version="1">MFLKTGDKVRVIAGKDKGQEGTVKKTLASENRVIVEGVNVVKKHQKPSNVNPNGGIIDQEAPINASNVMLLDPSTNEPTRVGYQVVDGKKVRVAKKSGQQID</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIU9</accession>
    <name>H4GIU9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">30S ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpsQ</name>
        <name evidence="6" type="ORF">PS3_15361</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3 4">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000023">
        <property type="protein sequence ID" value="EHS87179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121982.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000023.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15361"/>
    <dbReference type="EnsemblBacteria" id="EHS87179">
        <property type="protein sequence ID" value="EHS87179"/>
        <property type="gene ID" value="PS3_15361"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.531"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_B">
        <property type="entry name" value="Ribosomal_S17_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019984">
        <property type="entry name" value="Ribosomal_S17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_S17/S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_S17_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="PTHR10744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03635">
        <property type="entry name" value="uS17_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 3 5" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS87179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="88" mass="10204" checksum="31AC85C239236D02" modified="2012-04-18" version="1">MSDERNARKVYQGRVVSDKMDKTITVVIDTYKSAPIYGKRVKYSKKFYAHDENNEAHIGDTVQIMETRPLSAKKRFRLVKVVEKAIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIW3</accession>
    <name>H4GIW3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Deoxyribonucleoside regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_17529</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SorC transcriptional regulatory family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000024">
        <property type="protein sequence ID" value="EHS87135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007121996.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000024.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_17529"/>
    <dbReference type="EnsemblBacteria" id="EHS87135">
        <property type="protein sequence ID" value="EHS87135"/>
        <property type="gene ID" value="PS3_17529"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.548"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007324">
        <property type="entry name" value="Sugar-bd_dom_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04198">
        <property type="entry name" value="Sugar-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sugar-bind" evidence="2">
        <location>
            <begin position="58"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="319" mass="35381" checksum="8464EF0038ED0C32" modified="2012-04-18" version="1">MTTNKNRQLALQVSRLYYLDQLSQTQIAKQLQLSRPTISRLLQMAQAEGIVQIQINDPFTNRVDLEEDLKSKFHLKDCRIVDQTALDESQILEQLGQAGATYLQSILKDGDILGLTWGKTMAHLAKYLQPTTLQNLTAVYLKGTVANSSASNYSSEITKAVNRAFNTQTEILPVPVIFGHQQTRDLVSQDRFIHQLLDLGRQANIALYTVGTTRPSAMLFQLGYFDQTQIDSLMDQSVGDLLSQFIDLDGQIVDPNLNQRTMALPLDELKQKDYSILVAGGTAKLKAIQGALKGGYPNVLVTDINTAKNLLDVKKGTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GIZ3</accession>
    <name>H4GIZ3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_13419</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122026.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13419"/>
    <dbReference type="EnsemblBacteria" id="EHS87105">
        <property type="protein sequence ID" value="EHS87105"/>
        <property type="gene ID" value="PS3_13419"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.597"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1 2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="446" mass="48313" checksum="EF7DB39AED7DF583" modified="2012-04-18" version="1">MDTSESKKLLDVEAQAFSSAARIKYYDIVIDHGKGAIITDVDGNDYIDLLASASSTNTGHAHPKVVKAVTDQAQKLLQYTPAYFANTNGAKLAPKLASLAPMSGPVEVAWGNSGSDANDAIIKFSRAYTGRQYIVTFTGAYHGSTYGSMTASSVSLNMTRKMGPLLPGIVKVPFPDPWERESGESEANFVDRMFRAFKLPFETYLPAEEVAAILIEPIQGDGGLIKVPDAYMHKIYDFAKANGILFCIDEINQGMGRTGKWWSIQNFDGIEPDLMSVGKSLASGLPLSAVVGRQEIMESLGAPGHLFTTAGNPVTTAASLATFEVIEEEHLVERSAELGKKAKAFFDQEAAKYDFIGDVRMYGLNGGIDIIDPETGQGDSEATTKLMYRAFELGVIIISLRGNILRFQPPLVITEEQLDQAFDVLDQAFSELAAGKLELPAGHMGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ11</accession>
    <name>H4GJ11_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminotransferase</fullName>
            <ecNumber evidence="2">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_11842</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000026">
        <property type="protein sequence ID" value="EHS87123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122044.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000026.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11842"/>
    <dbReference type="EnsemblBacteria" id="EHS87123">
        <property type="protein sequence ID" value="EHS87123"/>
        <property type="gene ID" value="PS3_11842"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.628"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="3">
        <location>
            <begin position="37"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS87123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="393" mass="43661" checksum="6389B535E777CA60" modified="2012-04-18" version="1">MSDINTTLLQNMRRELANIKPSAIRSFNKEVSSIPGIIKLTLGEPDFDTPQHIKDAAKASIDNNHTHYPPTPGTDGLRAAAANFLNTKYNLNYKPTQILATVGATEAIWSSLYAITNPGDQVIVPTPIWPMYLSIVNGMGLKPILVNTEPNDFVLSAQMIEDTLAQHNHVKAVLLNFPSNPTGKTYSREAIEEIADVLRDKEIFMVSDEIYSELTYGKTHTSVAEILPEQTILLNGVSKSHAMTGWRLGIVAAPEGIIDKIITVHQFTVTTVPYTTQDAGEEAFSNGIDDALPMREEYIKRANFVHEAMTKMGFRADKPEGAFYYFAKLPEWLDEDTATFAHNLAYEQAVALVDATPFGPGAEGYIRISYASSMEDLQEAMKRIQKYLDSRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ45</accession>
    <name>H4GJ45_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8 10">Thymidine kinase</fullName>
            <ecNumber evidence="2 8 10">2.7.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tdk</name>
        <name evidence="12" type="ORF">PS3_11486</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>ATP + thymidine = ADP + dTMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19129"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17748"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.21"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 11">Belongs to the thymidine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.21" evidence="2 8 10"/>
    <dbReference type="EMBL" id="AICN01000028">
        <property type="protein sequence ID" value="EHS87070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122078.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000028.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11486"/>
    <dbReference type="EnsemblBacteria" id="EHS87070">
        <property type="protein sequence ID" value="EHS87070"/>
        <property type="gene ID" value="PS3_11486"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.684"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004797">
        <property type="term" value="F:thymidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00124">
        <property type="entry name" value="Thymidine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001267">
        <property type="entry name" value="Thymidine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020633">
        <property type="entry name" value="Thymidine_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11441">
        <property type="entry name" value="PTHR11441"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00265">
        <property type="entry name" value="TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF035805">
        <property type="entry name" value="TK_cell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00603">
        <property type="entry name" value="TK_CELLULAR_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 10" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="6 8 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8 9">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR035805-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS87070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="197" mass="22642" checksum="3FDBF74000F13302" modified="2012-04-18" version="1">MAQLFFKYGAMNSGKSMDILKVAHNYEEQGKPVLLMTSGVDDRSGQGMIASRVGLEREVTPIYADTNVFDLIKQTDQQIYCVLVDEAQFLKREHIVQLAKVVDELNIPVMAYGLKNDFRNELFEGSKYLLIYADKIEEIKTICWFCSHKALMNLRINNGKPVYEGEQIQIGGNESYYPVCRKHYFHPDLPCETEEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ67</accession>
    <name>H4GJ67_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Gamma-glutamyl phosphate reductase</fullName>
            <shortName evidence="7">GPR</shortName>
            <ecNumber evidence="7">1.2.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glutamate-5-semialdehyde dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Glutamyl-gamma-semialdehyde dehydrogenase</fullName>
            <shortName evidence="7">GSA dehydrogenase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">proA</name>
        <name evidence="10" type="ORF">PS3_6759</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the NADPH-dependent reduction of L-glutamate 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>L-glutamate 5-semialdehyde + NADP(+) + phosphate = H(+) + L-glutamyl 5-phosphate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the gamma-glutamyl phosphate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.41" evidence="7"/>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122100.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6759"/>
    <dbReference type="EnsemblBacteria" id="EHS87022">
        <property type="protein sequence ID" value="EHS87022"/>
        <property type="gene ID" value="PS3_6759"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.714"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004350">
        <property type="term" value="F:glutamate-5-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07079">
        <property type="entry name" value="ALDH_F18-19_ProA-GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00412">
        <property type="entry name" value="ProA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020593">
        <property type="entry name" value="G-glutamylP_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012134">
        <property type="entry name" value="Glu-5-SA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000965">
        <property type="entry name" value="GPR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000151">
        <property type="entry name" value="GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00407">
        <property type="entry name" value="proA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01223">
        <property type="entry name" value="PROA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0521">NADP</keyword>
    <keyword evidence="6 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 7" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="9">
        <location>
            <begin position="4"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Aldedh" evidence="9">
        <location>
            <begin position="313"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="3"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS87022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="413" mass="44914" checksum="097A02F70426AE34" modified="2012-04-18" version="1">MNLETMGEKAQVASRKLAQLTTEEKNQALRSMAQALEDHTENIIAANQRDLEAAVGLKASFVDRLTLDRARIVGMADGLRQLARLADPIGDVVDAWTTPTGLEIAKRRVPLGVIGMIFEARPNVSVDASGLCFKSGNAVILRGGKEALQTNQMLVKVLRRVLADHGLDPNAVQLIEDTSHEVANQFMQLTEYLDVLIPRGSARLINAVAQQATVPVIKTGAGNCHIYVDDDADLAMTINVVVNAKVQRPSVCNAAEKLLINSAVASEYLPAIAQALIDQKVELRGDKRAQAILGDQINPATSEDWDTEYNDYIMAIKVVDTLDEAISHINQHNTQHSEAIITNNYAHSQRFLAEVDAAAVYVNASTRFTDGSEFGFGAEIGISTQKLHARGPMGLKELTTTKYVIRGNGQVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ86</accession>
    <name>H4GJ86_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Iron compound ABC transporter permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_9433</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122119.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000029.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9433"/>
    <dbReference type="EnsemblBacteria" id="EHS87041">
        <property type="protein sequence ID" value="EHS87041"/>
        <property type="gene ID" value="PS3_9433"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.745"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="249"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS87041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="306" mass="32271" checksum="8A1D9BC3F33A7C2F" modified="2012-04-18" version="1">MKRIGWAYLGVMILLVVTIVIAVTCGSSSISWQHMTMTDYNTIFNIRLPRLVADLVGGAALAISGAFYQATFRNPIADAGILGISAGADLFAVVAALVIPGAFWFKVGGALIGGTIVLVVLVTLQQFLSPTQLILVGVAMDAAITGVQKILNTNQLNGLATVTWTGTMVLAVVGVIVIIAALFVAHWGNYLKVSDEFLESVGLNPRLLRTSLLIIATILATCTTAVIGIVAFLGIIVPQSCRVIIGRNYQNLIPFAGLCGAWLLLATDTFGRLIVQPSEISAQIILAIVGGPFLIVMLVKGKRAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="20" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJ92</accession>
    <name>H4GJ92_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_16443</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000029">
        <property type="protein sequence ID" value="EHS87047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16443"/>
    <dbReference type="EnsemblBacteria" id="EHS87047">
        <property type="protein sequence ID" value="EHS87047"/>
        <property type="gene ID" value="PS3_16443"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.756"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06259">
        <property type="entry name" value="YdcF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003848">
        <property type="entry name" value="DUF218"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02698">
        <property type="entry name" value="DUF218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="DUF218" evidence="2">
        <location>
            <begin position="160"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS87047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="328" mass="36725" checksum="53441ABF01ECC043" modified="2012-04-18" version="1">MSFGAFGTIWHHDRRSLFLGFYFLTTLGMLGVDLLVVNAQLWQAGSQFAWLVLVVIVLIVILLLLVPFLTSFLLIINGIQLIRRTRRSFNNLLSLLAGCLLLINLTIWAQLDHLFASWGPFFWHLYHMVDLVIADVLFLLTIYTVTSLLNLIHFKHEPLDYLIVLGAGLYQGRVSPLLAGRVQKAASLAQDNPQTKFIMSGGQGSDEPRSEAAAMKEYAMSLGVPVDRIILEDQSTNTAENIKFSQRLIPPNQKVGLVTNNFHLLRALLLSRQAGLPCVGYGSKSRFYYSLNAFIREFAGYLLLRKKLLVGILILIEGAYLLFATLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJA3</accession>
    <name>H4GJA3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative phage terminase large subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_2254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000031">
        <property type="protein sequence ID" value="EHS87015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122136.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000031.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2254"/>
    <dbReference type="EnsemblBacteria" id="EHS87015">
        <property type="protein sequence ID" value="EHS87015"/>
        <property type="gene ID" value="PS3_2254"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.787"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006437">
        <property type="entry name" value="Phage_terminase_lsu"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01547">
        <property type="entry name" value="phage_term_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HintN" evidence="1">
        <location>
            <begin position="382"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="482" mass="54823" checksum="804EF04924428528" modified="2012-04-18" version="1">MALNDYFNKKQLEVVKDYLTKDFGMMILSGAVRSGKTFVDNWLFIMELKRVSKLAKQRGDKHPQVILAGYSSNTIYNNVISSIENQMGLELKPDKHGHYHLYNVDIVPAYTGNARGMSAIRGMTSYSAYINEASLSTPEAFQEIVNRCSVRGSHIICDTNPNNPQHWLKVNYIDNDDPETKLLYYNFTIDDNTRLDQDYVSRLKASTPSGMFYDRMIKGLWVSGDGVVYSDFDKSKMVIGPDQIPDGLTYYCGVDWGYEHNGVITLWGDDDYGNSVLIKEINAKHQFIDWWINQAKQLQADYGKRLVFFADSARPDNVSAFIKAGIHTVNANKSILPGIEYVASLMKQGRFFINSQGYDQILDELYNYIWDEKTGQPVKEHDHCLTGDTQVWTADGYRPIKDLIGKTGMVNCIDTATNVPTQSRFSDARCTRHNAKIYKVTLEDGTIIRGTDDHKVYTTNGWKTIGELTSDDRIVRIQNSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJA4</accession>
    <name>H4GJA4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA replication protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_2256</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000031">
        <property type="protein sequence ID" value="EHS87016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122137.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000031.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_2256"/>
    <dbReference type="EnsemblBacteria" id="EHS87016">
        <property type="protein sequence ID" value="EHS87016"/>
        <property type="gene ID" value="PS3_2256"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.791"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase_AAA_core" evidence="1">
        <location>
            <begin position="97"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS87016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="262" mass="29995" checksum="10827ED714E868EA" modified="2012-04-18" version="1">MDKVAINQPQQPLTAEEIERVKTRINGIERSWGKMWTDKLKDQIEQQSLWSGGQQLHFSFNDWNPNRQPDSQLGADIGNQAYMLAKKMEQEPINVNLFGNRGTGKTSLALAMIDKVSKDSDKSWLFINTVELANLISRSYQYPESREQLNELYKAITGIRSGDGSWYRKPVDVLVLDDFGTEGGQNSDELRVRRDMQDWLYRASNARIDLQNNQPTGSTIITTNNTSNELLRMYDPKLISRIVTKNPQHVIDFTKAKDVRNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJB4</accession>
    <name>H4GJB4_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PS3_14036</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000032">
        <property type="protein sequence ID" value="EHS87004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122147.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000032.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14036"/>
    <dbReference type="EnsemblBacteria" id="EHS87004">
        <property type="protein sequence ID" value="EHS87004"/>
        <property type="gene ID" value="PS3_14036"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.807"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="10">
        <location>
            <begin position="67"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="127"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS87004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="336" mass="38417" checksum="D30161843097176D" modified="2012-04-18" version="1">MKRNKPFTRWTIVGVVITSVLMIMGMQLAMVLLTSEIPTWTNRQLLALMMIVPVFIILWIFLTFIFTKLTRIIRQLSRQMNRVASGDFSVRLDLKQSGPLRETYRDFNKMAQELEGVSKLRDDFISEFSHEFRTPITSINGFAEMLKDENLNEEEKRLYVDVIVAESNRLTNLSKNILLLTSLEKQTIVTDVQIYSLDEQLRQSVIAFLPQIEAKKIELEVDLVKTKLTSNQDLIQHIWTNLLNNCLKFTPENGKITVKNQVLTGKNRVVITNSGPNLSQEDQEKLFNKFYHAKDSSDGLGLGLAIVKRVLDLVGGKITVNNLPKTGVEFIVELNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJC5</accession>
    <name>H4GJC5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphomethylpyrimidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_20416</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThiD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000033">
        <property type="protein sequence ID" value="EHS86994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_20416"/>
    <dbReference type="EnsemblBacteria" id="EHS86994">
        <property type="protein sequence ID" value="EHS86994"/>
        <property type="gene ID" value="PS3_20416"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.827"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045029">
        <property type="entry name" value="HMP/HMP-P_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PTHR20858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phos_pyr_kin" evidence="2">
        <location>
            <begin position="19"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS86994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="275" mass="29942" checksum="746D47C7C7709144" modified="2012-04-18" version="1">MEDKKMTQYVQALTIAGHDSDGSAGMPADLHAFFIDQVYGMGIMTAVVSGNSYGITQQQLMPTDFIKEQFKVLAADFEIKAAKTGMLGTIDIINTVADCYDPQHFGKLVVDPVIITKHGVMLLEQAAYEAFREKIVPLAEVITPNFFEAQKLAEMEIKTDADMVTAAHRLQALGAKNVVIKGQHEAGWTDPVKDLLLKADGSQQWFAEEYVKTTHINGTGDTYSAIIAGELAKGNDVETAVQKAKRVVHAAIANEIAVGHKFGPINHWAGQDVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJI8</accession>
    <name>H4GJI8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Anaerobic ribonucleotide reductase activator protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_5742</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000041">
        <property type="protein sequence ID" value="EHS86578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122221.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000041.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5742"/>
    <dbReference type="EnsemblBacteria" id="EHS86578">
        <property type="protein sequence ID" value="EHS86578"/>
        <property type="gene ID" value="PS3_5742"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.947"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043365">
        <property type="term" value="F:[formate-C-acetyltransferase]-activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012837">
        <property type="entry name" value="NrdG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PTHR30352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352:SF2">
        <property type="entry name" value="PTHR30352:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00299">
        <property type="entry name" value="anaerobic_ribonucleoside-triph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02491">
        <property type="entry name" value="NrdG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="223" mass="25746" checksum="26F22F6635745B7D" modified="2012-04-18" version="1">MSNEIRISINGDIQFVDPNKLQGTDKTRRPKRRRQRQPKNPAPKEWLASDLSKMMVADYKPFNFVDGEGIRCSIYVSGCKFACPGCYNVVAQNFNYGFPYTDELEDQIMQDLSQPYVQGLTFLGGEPFVNTQICLRLAHRVRQEFGHSKDLWSWSGYTWEELLQDSEDKLEFLQLLDILVDGRFIEPLKDLTLQFRGSSNQRIIDVPKSLASGDVVIWDKLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJI9</accession>
    <name>H4GJI9_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 16">3-isopropylmalate dehydrogenase</fullName>
            <ecNumber evidence="6 16">1.1.1.85</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PS3_12182</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:32271"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.85"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="16">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="16">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 16">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.85" evidence="6 16"/>
    <dbReference type="EMBL" id="AICN01000041">
        <property type="protein sequence ID" value="EHS86579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122222.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000041.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12182"/>
    <dbReference type="EnsemblBacteria" id="EHS86579">
        <property type="protein sequence ID" value="EHS86579"/>
        <property type="gene ID" value="PS3_12182"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.949"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00072"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004429">
        <property type="entry name" value="Isopropylmalate_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42979">
        <property type="entry name" value="PTHR42979"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00169">
        <property type="entry name" value="leuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="14 16" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8 16" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0520">NAD</keyword>
    <keyword evidence="12 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iso_dh" evidence="17">
        <location>
            <begin position="4"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EHS86579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="346" mass="37522" checksum="84411DC1D382B1CA" modified="2012-04-18" version="1">MPNRIAALYGDYSGPEMMRAGLAVLKAVSQKNGFEYQIEAYPFGGEAIDQVGEPLPDQTLAGCQAADAILLSAIGGPKWSVGGQSPEAGLLKIRQALGLFANIRPTHIDQAIIDQSPVKASVLENTDFVIVRELTSGIYFGQPREEGIDQALDTTIYRRAEVERIVRVAFEYAKGRQQHLTVVDKANVLATSRMWRAIVKEVQPAYPEVEVDYMYVDAASMKIIAEPSFFDVIVTDNLFGDILSDEAAVLPGSLGMIPSISQGASGKALYEPIHGSAPTLANQDRINPISMIRSVSLMLRYSFERADLADQIEVAIAKTLSQGIMTADIGGQAKTSEMVSKIIQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJJ3</accession>
    <name>H4GJJ3_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GMP synthase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="9">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">GMP synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">guaA</name>
        <name evidence="12" type="ORF">PS3_10161</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2 H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="9"/>
    <dbReference type="EMBL" id="AICN01000042">
        <property type="protein sequence ID" value="EHS86503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122226.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000042.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_10161"/>
    <dbReference type="EnsemblBacteria" id="EHS86503">
        <property type="protein sequence ID" value="EHS86503"/>
        <property type="gene ID" value="PS3_10161"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.962"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003922">
        <property type="term" value="F:GMP synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00344">
        <property type="entry name" value="GMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022955">
        <property type="entry name" value="GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 9 10" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMPS ATP-PPase" evidence="11">
        <location>
            <begin position="203"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="230"/>
            <end position="236"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS86503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="517" mass="57120" checksum="FE9AD2EF4DB6D246" modified="2012-04-18" version="1">MAKVNLDNFDKILVLDFGSQYNQLITRRIRDFGIYSELLSHKLSAQEIQAMNPKGIIFSGGPNSVYDEDAFKVDPEIFKLGIPILGICYGMQLMAYDLGGKVEAADDSEYGRADITVVDDQAVMFNGLPKEQAVWMSHGDKVTAVPDGFHVVATSQNCPIAAMADDDRKFYGLQFHTEVRNTDHGLEILKKFAFDVCGAQANWSMDDFIDLQVNSIREEVGDKKVILGLSGGVDSSVTAALLNRAIGDQLTAIFVDHGLLRKNEADEVMGALADGLGINIVKVDAADRFLGKLQGVIDPEKKRKIIGNEFVEVFNDEARKLKDADFLAQGTLYTDVIESGTDTAQTIKSHHNVGGLPEELGFKLIEPLRSLFKDETRVLGEKLGIPYDLVWRQPFPGPGLGIRVIGEITPEKLALVRDADAILREEVKKAGLDEEIWQYFVALPGMRSVGIMGDGRTYDYAVCIRAVTSIDGMTADFAQIPWDILQKISVRIVNEVDHINRVLYDVTSKPPATIEYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJT1</accession>
    <name>H4GJT1_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid ABC superfamily ATP binding cassette transporter, binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PS3_5949</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial solute-binding protein 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5949"/>
    <dbReference type="EnsemblBacteria" id="EHS86118">
        <property type="protein sequence ID" value="EHS86118"/>
        <property type="gene ID" value="PS3_5949"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1108"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018313">
        <property type="entry name" value="SBP_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01039">
        <property type="entry name" value="SBP_BACTERIAL_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003591900" evidence="4">
        <location>
            <begin position="32"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="5">
        <location>
            <begin position="39"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="PBPe" evidence="6">
        <location>
            <begin position="39"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS86118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="263" mass="28453" checksum="F7C45780078E7E8C" modified="2012-04-18" version="1">MKRIKRLSVLAVAVVATVTLAACGKSSSSSQANLITKGTLTVGLEGTYAPYSYKENGKLKGFEVDLAQDVAKKLGYKVKFVPTKWDSLIAGLGSNKFDIVFNDLAETPQRKKAYAFSTPYIYSKSALITKKDSTISSIDDIKGQKVAAGTGTANESNVKKFGGTVVSSSDFASSMQLIQQGRVVAALNSNEAYAYYIKQNPNADLKSIEVPTSQIASQKITAMMSKNNKGLQKKVNKALKELRQDGTLKKLSEKYFGTDLTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJT5</accession>
    <name>H4GJT5_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_19332</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the auxin efflux carrier (TC 2.A.69) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000049">
        <property type="protein sequence ID" value="EHS86122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122318.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000049.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_19332"/>
    <dbReference type="EnsemblBacteria" id="EHS86122">
        <property type="protein sequence ID" value="EHS86122"/>
        <property type="gene ID" value="PS3_19332"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1112"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="307" mass="34042" checksum="53BBC0A52B2E78D7" modified="2012-04-18" version="1">MSISQLANQIMVLFLLMFLGALIRALGFFKVTTISEMTNMLLYFASPIVIFNSFQKDFSLNRLHILGLVLVAVIFSYIVMIVVAKLIFQRVQDPNLRRIARYGSVYCNNGFMGIPLALALFGNNGVFYGVASMAGFNLFSWTQGVGMFRQQQPSFHEQLKQIFKNPNIIAIILGLLAFVFSLKMPTMISTFMGDISSAYGPMSMIVIGCNLVGLNWRDLKLPRSMWITLLLRNLIFPIITILILRLLGVTGVALYTTVILSACPVAGVVVLFTLQAKDDTKPAVVLMSISTLLSLITIPICFALTML</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJY8</accession>
    <name>H4GJY8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HesB/YadR/YfhF-family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_13755</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS86005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122371.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13755"/>
    <dbReference type="EnsemblBacteria" id="EHS86005">
        <property type="protein sequence ID" value="EHS86005"/>
        <property type="gene ID" value="PS3_13755"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1193"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="SSF89360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS86005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="117" mass="13127" checksum="B4425BDD8B43499D" modified="2012-04-18" version="1">MSEITKTKITLTPQISEWFRKEMNLEPGNGIRFFGRVYGATNAHTGFSAAMSRCDQPNSPVVDEVIDGIHYYIQDSDFWFFNGLDLEVSYDEAIDGPAYHFKPNDGSKLDAKASASH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-05-25" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>H4GJZ1</accession>
    <name>H4GJZ1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6 7">50S ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rplS</name>
        <name evidence="8" type="ORF">PS3_4946</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6 7">This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the bacterial ribosomal protein bL19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000051">
        <property type="protein sequence ID" value="EHS86008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122374.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000051.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_4946"/>
    <dbReference type="EnsemblBacteria" id="EHS86008">
        <property type="protein sequence ID" value="EHS86008"/>
        <property type="gene ID" value="PS3_4946"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1201"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00402">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038657">
        <property type="entry name" value="L19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001857">
        <property type="entry name" value="Ribosomal_L19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018257">
        <property type="entry name" value="Ribosomal_L19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680">
        <property type="entry name" value="PTHR15680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01245">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002191">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00061">
        <property type="entry name" value="RIBOSOMALL19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01024">
        <property type="entry name" value="rplS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01015">
        <property type="entry name" value="RIBOSOMAL_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS86008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="118" mass="13467" checksum="7CD442E608956CE9" modified="2012-04-18" version="1">MRQNQLIEKITASQLRSDIPDFRAGDTVRVNALIVEGSRERVQVFEGVVIKRHGSGISATYTVRKISNGVGVERTFPLHSPRVEKIEVVRHGKVRRAKLYYLRERTGKSARIAERRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK08</accession>
    <name>H4GK08_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0297 protein PS3_11216</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_11216</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0297 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000054">
        <property type="protein sequence ID" value="EHS85860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_11216"/>
    <dbReference type="EnsemblBacteria" id="EHS85860">
        <property type="protein sequence ID" value="EHS85860"/>
        <property type="gene ID" value="PS3_11216"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1234"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01507">
        <property type="entry name" value="UPF0297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009309">
        <property type="entry name" value="IreB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40067">
        <property type="entry name" value="PTHR40067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06135">
        <property type="entry name" value="IreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037258">
        <property type="entry name" value="DUF965_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="92" mass="10711" checksum="B4BC4B84A78D7450" modified="2012-04-18" version="1">MEVTIMATDETMFYDFNQGRPQSVEATLKTVYDALEEKGYKPINQIVGYLMSGDPAYIPRLHDARNLIRQYERDEIVEELVRSYLHHNGVTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK09</accession>
    <name>H4GK09_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative pre-16S rRNA nuclease</fullName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_13228</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Could be a nuclease involved in processing of the 5'-end of pre-16S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YqgF HJR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="AICN01000054">
        <property type="protein sequence ID" value="EHS85861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122392.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000054.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13228"/>
    <dbReference type="EnsemblBacteria" id="EHS85861">
        <property type="protein sequence ID" value="EHS85861"/>
        <property type="gene ID" value="PS3_13228"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1235"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000967">
        <property type="term" value="P:rRNA 5'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16964">
        <property type="entry name" value="YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00651">
        <property type="entry name" value="Nuclease_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005227">
        <property type="entry name" value="YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006641">
        <property type="entry name" value="YqgF/RNaseH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33317">
        <property type="entry name" value="PTHR33317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03652">
        <property type="entry name" value="RuvX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00732">
        <property type="entry name" value="YqgFc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00250">
        <property type="entry name" value="RNAse_H_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="YqgFc" evidence="3">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="146" mass="16353" checksum="98022E083D324300" modified="2012-04-18" version="1">MRLLGLDVGSKTVGVAQSDPLGWTAQAVEIIPINETEEVFGLERIAELVKERQVAGFVIGLPKNMNNTEGPRVEASRHYGELLQAKFPNLPVDYQDERLTTVQASRMLIEEADVSRRKQKQVIDELAATLILQNYLDRHGKLTTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK15</accession>
    <name>H4GK15_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dITP/XTP pyrophosphatase</fullName>
            <ecNumber evidence="6">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="6">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_9946</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>H2O + ITP = diphosphate + H(+) + IMP</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>H2O + XTP = diphosphate + H(+) + XMP</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000054">
        <property type="protein sequence ID" value="EHS85867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122398.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000054.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9946"/>
    <dbReference type="EnsemblBacteria" id="EHS85867">
        <property type="protein sequence ID" value="EHS85867"/>
        <property type="gene ID" value="PS3_9946"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1241"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:nucleoside-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009146">
        <property type="term" value="P:purine nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01405">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020922">
        <property type="entry name" value="dITP/XTP_pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="Ham1p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="PTHR11067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00042">
        <property type="entry name" value="TIGR00042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="8"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="151"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="179"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="196" mass="21620" checksum="E4C222696E6C2C36" modified="2012-04-18" version="1">MASIIIATKNAHKAQEFAALLAPYQLEVKTLADFPPLEIVEDGRTFEENARKKAQAAVAAFNLPVLADDSGLMIDALNGEPGVYSARYAGDHDDAANNQKVLEKLNGLPFAERSAHFHTTIFGLKPTGEEIVVNGQVNGHILDTPQGENGFGYDPIFWVDELEVPMATLSMTEKNQISHRGRALQALMQEFKEWWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK43</accession>
    <name>H4GK43_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Avirulence D protein-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PS3_21971</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000056">
        <property type="protein sequence ID" value="EHS85810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122426.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000056.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="EHS85810">
        <property type="protein sequence ID" value="EHS85810"/>
        <property type="gene ID" value="PS3_21971"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1279"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008799">
        <property type="entry name" value="Pseudomon_AvrD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05655">
        <property type="entry name" value="AvrD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS85810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="299" mass="33803" checksum="6E379E0F65F25AB6" modified="2012-04-18" version="1">MNVDTILGDRNTRYFGVGYKNANYEIITLDQEESEAMVKVNFGDEVWSIKQGEARSPHLSTLDSVIISAMIVEQLLGPEKSADYYVSHFDIRAGGEPVELSKALKVDFTQNGNNFHFNILGMKINLSIRFGNHISNSDLGHESFLTQHLKQSELTIDGIDYLDQTSMAAVAVKQAEGNDYAGLGSKTNDNGFSIFEWLIIFSQIGEMLTYQLDNLDRDDCANLWMKRISADYQDRVVNKKQLQRSRVIGEVLKSRVIDKDHHQWRTLTAHGSDINNQVYFSAQMAHELPQVMEGSSNEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK48</accession>
    <name>H4GK48_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PS3_13776</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">PS3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000057">
        <property type="protein sequence ID" value="EHS85801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13776"/>
    <dbReference type="EnsemblBacteria" id="EHS85801">
        <property type="protein sequence ID" value="EHS85801"/>
        <property type="gene ID" value="PS3_13776"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1289"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13124">
        <property type="entry name" value="MATE_SpoVB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024923">
        <property type="entry name" value="PG_synth_SpoVB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038958">
        <property type="entry name" value="PG_synth_SpoVB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="413"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="476"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="504"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="382"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS85801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="549" mass="60337" checksum="958A11945DECFDD3" modified="2012-04-18" version="1">MKAEAPEHQSEVSSTSDATRKMVTGSAWMTAGSITSRILGALYVIPWVTWMGVYSDQANAAYAKGYNIYQYFIILASAGIPSAISQMVADYNGRGQYLTSKRLYRSAMYVAMFTGIFFMSVMYFGAGILAQGDANVVPVIRALAWAILIIPAMSISRGYLQGYNWMAPSAISQFIEQLLRVGYMLGATYFVMKIGSGNWVSATAQSTFAAFIGAIGACLVLLVAELRERREIDELVKTSHGDQDISTLKLIGQIIYRAIPFIIIESGISLFLLIDQYTFPVIMHMTGTYTKLQVNTLYALFAFNTNKIYMIIVSLGTALAATAIPLLASARAKNNLAETQHQIEKGLLLFYFVMIPASLGLSAVAQPVYTVFYRYSAAGTAVMSFAAFMSIPYGVYTVAAAMMQGISENKKMMTYLGIGIIIKFILQYPFVKWFTGLGPLLSTAVAMLIVIGMVYGTFHRRYGVHFSEMTKPTNQIIIASLIMYVIVRVVMWGIGLFISPYGRYTAFFSLIPGVAIGGIVFAYLVLKFGVAEYLLGPRINGLKAKLHIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK61</accession>
    <name>H4GK61_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_16210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000059">
        <property type="protein sequence ID" value="EHS85695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122444.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000059.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16210"/>
    <dbReference type="EnsemblBacteria" id="EHS85695">
        <property type="protein sequence ID" value="EHS85695"/>
        <property type="gene ID" value="PS3_16210"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1306"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011426">
        <property type="entry name" value="CamS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07537">
        <property type="entry name" value="CamS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF012509">
        <property type="entry name" value="CamS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003592924" evidence="1">
        <location>
            <begin position="22"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="369" mass="40662" checksum="0E3A5F7AC717F2D5" modified="2012-04-18" version="1">MKLLKRMLTVGMLAGLSIGLAACSTSSKSKVTTLSSGGSGNYRTVVVNGKYRTSKSRGVNSSNGNSTASLKSFDSGLTTVAKRIFSTKTYIFQEGQYLSSTTINHWLARVSSNAAGLNPKKGSSDDPNPEYIQQIEEQDYMTQDGNNLKLRGMVIGIAVNSEYQYQKETDGPYYTKTISKAEGLAKGKEAAAKILKRLRKNSKLKNIPIVIAIYRQASSDSLVGGNYVAYSRNNGSTISEWHKLNYSTKILPKSSDATSTTEDDQSDDSNFTNFKNKVQNFFPNISGVTAQAQYYNGELSGMNISVTTQFYSSSEIQSFTQYIERQAKKYLPSGIPIEIKIQTSDNEIQSVVYRNSNSDDYESHVFSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK82</accession>
    <name>H4GK82_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative intracellular protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_9478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122465.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9478"/>
    <dbReference type="EnsemblBacteria" id="EHS85534">
        <property type="protein sequence ID" value="EHS85534"/>
        <property type="gene ID" value="PS3_9478"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1341"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006287">
        <property type="entry name" value="DJ-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01383">
        <property type="entry name" value="not_thiJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1_PfpI" evidence="1">
        <location>
            <begin position="3"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="194" mass="21424" checksum="1DEE7FA37FF6C90C" modified="2012-04-18" version="1">MAKEVAVIFADGCEEVEGLTVVDVLRRLEIPTKMVGLTDRQVMGAHQIALTCDAVVSDDLLTADVVAFPGGRGGAERLRDHEALKSIMVQRQNDGKWNAAMCAAPIAFARYGLFKDADFTCFPGFDQVILSEEPSARFHEDITVVDDQRHLITSRGPATAMAFAYEIAEQLGVDTQELQHAMLYDYLQAEFQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>H4GK96</accession>
    <name>H4GK96_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fumarate hydratase class II</fullName>
            <shortName evidence="3">Fumarase C</shortName>
            <ecNumber evidence="3">4.2.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Aerobic fumarase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Iron-independent fumarase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fumC</name>
        <name evidence="6" type="ORF">PS3_15040</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(S)-malate = fumarate + H2O</text>
            <dbReference type="Rhea" id="RHEA:12460"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="EC" id="4.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="3">There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.2" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122479.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15040"/>
    <dbReference type="EnsemblBacteria" id="EHS85548">
        <property type="protein sequence ID" value="EHS85548"/>
        <property type="gene ID" value="PS3_15040"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1356"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045239">
        <property type="term" value="C:tricarboxylic acid cycle enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004333">
        <property type="term" value="F:fumarate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006106">
        <property type="term" value="P:fumarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01362">
        <property type="entry name" value="Fumarase_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00743">
        <property type="entry name" value="FumaraseC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005677">
        <property type="entry name" value="Fum_hydII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018951">
        <property type="entry name" value="Fumarase_C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11444">
        <property type="entry name" value="PTHR11444"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10415">
        <property type="entry name" value="FumaraseC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lyase_1" evidence="4">
        <location>
            <begin position="12"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="FumaraseC_C" evidence="5">
        <location>
            <begin position="405"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="100"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="136"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="320"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="314"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="3">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS85548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="462" mass="50404" checksum="4A6ADC0D833A588C" modified="2012-04-18" version="1">MVEYRTEEDTLGPVKIPADALWGPQTERSRHNFPTGQYMPLPVIRALLNIKKAAAQANLELAQIDEVRGNLIIEAIDSLLALDDEELRKDFPLKVYQTGSGTQTNMNTNEVVAHKAHELNPDVEILPNDHVNMGQSSNDTFPTAMNMAAVDAVAKLEKATQHLIDELKVKQDQYWRTVKVGRTHLQDATPLTFGQEVSGYVSALEHDLTYLENLRPTLLELAIGGTAVGTGLNAAPGMPEKIAEKVSAVYGHEFTAKSNKFYGLAHHSGLNVVHGAIKTLAADVFKIAQDIRFLASGPRAGYGELNIPANEPGSSIMPGKVNPTQAEAVTMAAMRVFGNDVVVSMSSSQGNFEMNVYKPVLIDAFLESADLLAGTMTGFADKMIHGLTVNEERMAELLDNSLMTVTALSPHIGYHDSAVIAQTADREGTTLREAALKSGKVTEAQYDEWMDYTKMTNIDREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKA2</accession>
    <name>H4GKA2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Membrane-associated phospholipid phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_3345</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122485.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3345"/>
    <dbReference type="EnsemblBacteria" id="EHS85554">
        <property type="protein sequence ID" value="EHS85554"/>
        <property type="gene ID" value="PS3_3345"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1369"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="2">
        <location>
            <begin position="75"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="191" mass="22085" checksum="D3CD12944C379CD6" modified="2012-04-18" version="1">MITWVVIVVLLVGFFTMMIGINRGWWRGLDQWAFLHVTNQQDHRFWHQVAFLGQPWLTVGWIIAISLWWLLQGQWLLTIWTLGVLGIANVIGIITKKVVSRTRPVGHLPHDDDFSFPSGHVLGLTLLVNLLIFYWPSWGVVLLAVLWWLLVASSRLVLRAHHLSDLIGTLCFAQAWFLICDKLLTLVWLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKB5</accession>
    <name>H4GKB5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aminopeptidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_9738</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122498.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_9738"/>
    <dbReference type="MEROPS" id="C01.086"/>
    <dbReference type="EnsemblBacteria" id="EHS85567">
        <property type="protein sequence ID" value="EHS85567"/>
        <property type="gene ID" value="PS3_9738"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1386"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00585">
        <property type="entry name" value="Peptidase_C1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004134">
        <property type="entry name" value="Peptidase_C1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363">
        <property type="entry name" value="PTHR10363"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03051">
        <property type="entry name" value="Peptidase_C1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005700">
        <property type="entry name" value="PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 4" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0788">Thiol protease</keyword>
    <feature type="active site" evidence="3">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR005700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR005700-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS85567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="444" mass="50838" checksum="B4318CB70186F280" modified="2012-04-18" version="1">MTLVISKEDLASFHHAYQARPESKVLERTVTKNGVQAASFDWHSIADNDQHFSIDLSTGEVADQKQSGRCWMFAALNTMRHDMQAKFNLPDDFELSQSYQFFWDKFEKANYFYENVLKTADLPTDDRRVAWLMTTPQQDGGQWDMLVALIEKYGVVPQSAMPESYNSSRSNEINGVLNQKLRHDAVILRDLVAQGASEADLTATRKTMMQDVYRMLAYAFGEPVANFDFEYRDKDKQYHREANLSPQSFFSKYVGWNLDDYISVIHAPTKDKEYYRTYTIEMLGNVVGSRPVKHLNLPMNEFKQLAVDQLKAGESVWFGSDVSKESERKLGIMALGTYGYEDLFDTQLTMSKEDALDYGQSMMNHAMVLTGVDLVDDQPTKWKVENSWGTKVGEKGYFVMSDAWMDKYCYQVVVNKRFLSDQQAAAQAQAPEVLKPWDPMGTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKB7</accession>
    <name>H4GKB7_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">2,3-bisphosphoglycerate-dependent phosphoglycerate mutase</fullName>
            <shortName evidence="4">BPG-dependent PGAM</shortName>
            <shortName evidence="4">PGAM</shortName>
            <shortName evidence="4">Phosphoglyceromutase</shortName>
            <shortName evidence="4">dPGM</shortName>
            <ecNumber evidence="4 5">5.4.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gpmA</name>
        <name evidence="6" type="ORF">PS3_22100</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.11" evidence="4 5"/>
    <dbReference type="EMBL" id="AICN01000062">
        <property type="protein sequence ID" value="EHS85569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122500.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000062.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_22100"/>
    <dbReference type="EnsemblBacteria" id="EHS85569">
        <property type="protein sequence ID" value="EHS85569"/>
        <property type="gene ID" value="PS3_22100"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1388"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046538">
        <property type="term" value="F:2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01039">
        <property type="entry name" value="PGAM_GpmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005952">
        <property type="entry name" value="Phosphogly_mut1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11931">
        <property type="entry name" value="PTHR11931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01258">
        <property type="entry name" value="pgm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="2 4" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="21"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="87"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="114"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="4">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="4">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS85569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="229" mass="26438" checksum="5A83E4A7785C719A" modified="2012-04-18" version="1">MVRLVLVRHGQSQANLDNIFTGWTDVPLTPLGYQQGIQVGQKFAQRQFSFTDVHTSYMQRAIITANLILEQLDLLDLPIHKTWRLNERHYGALSGQNKLAVKAKVGTAQFKAWRRGYRALPPRLDQPHHDRRYDRLGVQEPLSESLQMTLERLLPYWEDQIAPRLLDQRDQLIVAHGSSLRALIKYLEGIPDDQIDQVEVPNGRPIIYELNDHLVIQAKEIMDGEEYAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKD6</accession>
    <name>H4GKD6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_12358</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000063">
        <property type="protein sequence ID" value="EHS85500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_12358"/>
    <dbReference type="EnsemblBacteria" id="EHS85500">
        <property type="protein sequence ID" value="EHS85500"/>
        <property type="gene ID" value="PS3_12358"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1427"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="2">
        <location>
            <begin position="30"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="381" mass="43100" checksum="D57EDCD4087CDA8C" modified="2012-04-18" version="1">MEANQHRGEEHHQLPTAERMIDFASYSLHPERVAVAEIKQAVDHVLADAGGEAFTVNDPMGLFSLRQTIRDYLSHNQIVTSSDQIQITSGSQQAIDLCARTLLHPGDIVLMQNPSYSGAIDAFRASGAHIIGISPEPSEEFIATLEAKIKLLQPKLLYLMSNVANPTGLSLTDQQKHQVYGLAHHYDLWIIEDDYGVDVSGDWQPTTIKMFDRDERVIYLRSLSRVLAAGIRLGYMVLPQGMVERIVNAKQIADLSTSGLAQRIFEQCLTSQSWQQYLHRSQMAYQRQYWFVNQLLKESQLPITWQAVDHCSWFWLKLPVGRSAKDVCQQAQQAGVLVENGDRYFVDQQPDQYLRLSLAPAKDSLIQVGLERLIEIISRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKE3</accession>
    <name>H4GKE3_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Replication initiator protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_15218</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000064">
        <property type="protein sequence ID" value="EHS85435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122526.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000064.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15218"/>
    <dbReference type="EnsemblBacteria" id="EHS85435">
        <property type="protein sequence ID" value="EHS85435"/>
        <property type="gene ID" value="PS3_15218"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1443"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041151">
        <property type="entry name" value="Bac_RepA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010724">
        <property type="entry name" value="RepA_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18008">
        <property type="entry name" value="Bac_RepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06970">
        <property type="entry name" value="RepA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RepA_N" evidence="1">
        <location>
            <begin position="19"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Bac_RepA_C" evidence="2">
        <location>
            <begin position="254"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="370" mass="43346" checksum="6F19750ABE4100D3" modified="2012-04-18" version="1">MTKSTNFNYYEADNVYGALFFQFPKVLMYGEQYKHLSSDAKLAYMVLKDRLEYSLRNNWVDEEGHVYFTFTNQELENLFNCHNQKVVKIKKELETAHLLMQKQLGFNPKTKKNEPNRLYLSKLDVKATDVYLRGEDSPKVSQTLDTSGNVKITLPQETAQTLDTSGNVKITLPHKSVDETAQTLDTSGNVKITHNLYKDYKNNIDTNRYDIDTQKLDFSTTNFSPAEIEAQNRNLVKHADEFLTDEDNGLPVFLEPEAVQLLSFWCRTPQQMRRFIGIILNAKYAVEKEHKDLGVWILLDDPDLKKMMTKTLRRYFNALRSDEKHIKNVENYLYGTMQNLFDVWWNQQAAREYAAKHPKEQNIDNEYTWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKF6</accession>
    <name>H4GKF6_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative TetR-type transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PS3_13991</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000065">
        <property type="protein sequence ID" value="EHS85430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122538.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000065.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13991"/>
    <dbReference type="EnsemblBacteria" id="EHS85430">
        <property type="protein sequence ID" value="EHS85430"/>
        <property type="gene ID" value="PS3_13991"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1472"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="9"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS85430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="194" mass="22875" checksum="8C50381EFDBBA67B" modified="2012-04-18" version="1">MNGKQRMVEQSKQWLCDALINLMTKEDFQDISITEIAQTAELSRKTFYHSFKNKEAVINYLCDQLFDQYFEQLLQQKPQVGEPMLKTTFDIFFNFWWRKRDLIQLLIRQGLFDHVNEIWQQKAIPRYQQFAAPWHVHGTPTQVNYVMALQIGGLTNILRVWLGQDQPEPPEQIKDLMLLAAQQLADSLNTNQIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKI2</accession>
    <name>H4GKI2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_16412</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000069">
        <property type="protein sequence ID" value="EHS85262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_16412"/>
    <dbReference type="EnsemblBacteria" id="EHS85262">
        <property type="protein sequence ID" value="EHS85262"/>
        <property type="gene ID" value="PS3_16412"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1523"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="361"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="410" mass="47272" checksum="91E04E148377B864" modified="2012-04-18" version="1">MDTDRGLKMKTKLKKIWNTPITGAQLYLLAFAIYFIPAFLIDSLYTNYISWSKLRLLTYLAIPILIFKIYVMDRWRWPTKLFITGLILFSVIAWRASRYPELMVTMVFVLAASGIDFKQVARWYLYLSVTFLVIIAVTSLVHIVPNLVYTSALRPNRYGLGMAYTTYVASHALFSALAYCYVRFGKLGWLDYLGIAIVAFVTMKYTNTRLDFYAMLLMIPVMWITQRAANGKRYAQIISSFWWIATPVLAVITLTGSYFFDDHNQIYRKFNELLSGRLGLSKKAFSMYDPNIFGRYIQEKAFGGAKGQAFANQNQFGLSADYFYIDSSFVRMLLLWGIVIFLLFIVAMTFIALVNTIQRKYILSAIVMLVAINSMVEPHSLQLIYNVFILALIPQIEWVKHKLSGDNYVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKK8</accession>
    <name>H4GKK8_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ketol-acid reductoisomerase (NADP(+))</fullName>
            <shortName evidence="8">KARI</shortName>
            <ecNumber evidence="8">1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Acetohydroxy-acid isomeroreductase</fullName>
            <shortName evidence="8">AHIR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Alpha-keto-beta-hydroxylacyl reductoisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ilvC</name>
        <name evidence="12" type="ORF">PS3_7963</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:13493"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49256"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 9">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.86" evidence="8"/>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_7963"/>
    <dbReference type="EnsemblBacteria" id="EHS85177">
        <property type="protein sequence ID" value="EHS85177"/>
        <property type="gene ID" value="PS3_7963"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1563"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00435">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013023">
        <property type="entry name" value="KARI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000506">
        <property type="entry name" value="KARI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013116">
        <property type="entry name" value="KARI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014359">
        <property type="entry name" value="KARI_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="PTHR21371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="IlvN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000116">
        <property type="entry name" value="IlvC_gammaproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00465">
        <property type="entry name" value="ilvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8 9" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="8 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="10">
        <location>
            <begin position="2"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="11">
        <location>
            <begin position="182"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="25"/>
            <end position="28"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 9">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS85177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="335" mass="37178" checksum="9841DF3C7BD53AA1" modified="2012-04-18" version="1">MVEMLYEKDVTTNYLAGKNVAFIGYGAQGHAQANNLRDSGVEVVVGVRPGQSADKAKNDGFEVYSVAEAAKQADWVQMLTPDEVMADVYKNEVADNLEPGNVLGFSHGFNVYYKEIVPPADVDVVMMAPKGPGNLCRRTYKEGFGVPALYAVYQDHSGHAEDLAKEFAKANGAARAGLLATTFKEETDEDLFGEQNVLMGGITALIETGFEVLTEAGYSPELAYFEVEHEMKLICDLIYEGGFAKMYKDCSNTSEYGSYTRGPRVVNEESKRAMKQNLQEIQDGTFAKEFMDDYHNGFKKLYAYRSEMEQSQLQKVGDELRAKMPFVAAEDKYSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKL8</accession>
    <name>H4GKL8_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_6957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000071">
        <property type="protein sequence ID" value="EHS85187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122600.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000071.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6957"/>
    <dbReference type="EnsemblBacteria" id="EHS85187">
        <property type="protein sequence ID" value="EHS85187"/>
        <property type="gene ID" value="PS3_6957"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1575"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS85187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="167" mass="18797" checksum="64B53A4725267BAB" modified="2012-04-18" version="1">MSDEQVSLKLADQTDAKRVLSFLRQAAQETNVILIPHLESVNESVEALNLKTIAQQDDALVLLACLETEVIGMLTIMPLPDHQQAGELGIVVLKDYWHQGIGSLLIDEALYWYQNYAPLEHLVLDVFKSNSRAIRLYQHYGFVITGETEITDARGSQQPTILMEFTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKQ2</accession>
    <name>H4GKQ2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosome maturation factor RimP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rimP</name>
        <name evidence="6" type="ORF">PS3_14710</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for maturation of 30S ribosomal subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RimP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS84972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122634.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14710"/>
    <dbReference type="EnsemblBacteria" id="EHS84972">
        <property type="protein sequence ID" value="EHS84972"/>
        <property type="gene ID" value="PS3_14710"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1623"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01734">
        <property type="entry name" value="YlxS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01077">
        <property type="entry name" value="RimP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003728">
        <property type="entry name" value="Ribosome_maturation_RimP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028998">
        <property type="entry name" value="RimP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036847">
        <property type="entry name" value="RimP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028989">
        <property type="entry name" value="RimP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035956">
        <property type="entry name" value="RimP_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33867">
        <property type="entry name" value="PTHR33867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02576">
        <property type="entry name" value="DUF150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17384">
        <property type="entry name" value="DUF150_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74942">
        <property type="entry name" value="SSF74942"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75420">
        <property type="entry name" value="SSF75420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="DUF150" evidence="4">
        <location>
            <begin position="13"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="DUF150_C" evidence="5">
        <location>
            <begin position="90"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS84972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="159" mass="18136" checksum="BDB6D5F82DC88388" modified="2012-04-18" version="1">MSNNDVIAAVTALVTPILDQYDFELFDLEFTKEGQSWYLRVYIDKPGGITLEDCATVSDQLSEALDNVEPDPIPQAYFLEVSSPGAERPLKRERDYENALNEYINVSLYQAVEGKKVYEGTLIDLTPDKLTLDYLDKTRHKQITIDRQKIAKARLAIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKV2</accession>
    <name>H4GKV2_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 10 12">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="5 10 12">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hisA</name>
        <name evidence="13" type="ORF">PS3_8586</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">PS3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10 12">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 10 12">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10 11">Belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="5 10 12"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122684.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_8586"/>
    <dbReference type="EnsemblBacteria" id="EHS85022">
        <property type="protein sequence ID" value="EHS85022"/>
        <property type="gene ID" value="PS3_8586"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1684"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04732">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01014">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006063">
        <property type="entry name" value="HisA_bact_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023016">
        <property type="entry name" value="Isoase_HisA-like_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="PTHR43090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00007">
        <property type="entry name" value="TIGR00007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10 11" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="9 10 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="7"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS85022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="241" mass="25755" checksum="B7FF79555C4C4B1D" modified="2012-04-18" version="1">MIIPAIDMQNGKSVRLYQGDFAAETLINESPLQQAHQYQAAGIRVIHLVDLDGAKVGHPVNQAIVETITHQFSGQVEIGGGIRTAKDVQSYFELGIDRVILGSIALKDPDLTKKLLAQYGGEKIVIGVDGQNGQVATEGWLDQSQVAMGDLIGQMVAAGAKHFIVTDVSRDGAMQGPNVNLLADLQARFPGANIIASGGIRDLNDIKELQKQGIHDSVVGRSIYEGTISLAEIAEVNRHVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKW1</accession>
    <name>H4GKW1_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipoprotein signal peptidase</fullName>
            <ecNumber evidence="6">3.4.23.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Prolipoprotein signal peptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Signal peptidase II</fullName>
            <shortName evidence="6">SPase II</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">lspA</name>
        <name evidence="9" type="ORF">PS3_15051</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">This protein specifically catalyzes the removal of signal peptides from prolipoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains.</text>
            <dbReference type="EC" id="3.4.23.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; lipoprotein biosynthesis (signal peptide cleavage).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 8">Belongs to the peptidase A8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.36" evidence="6"/>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122693.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000072.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_15051"/>
    <dbReference type="EnsemblBacteria" id="EHS85031">
        <property type="protein sequence ID" value="EHS85031"/>
        <property type="gene ID" value="PS3_15051"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1695"/>
    <dbReference type="UniPathway" id="UPA00665"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00161">
        <property type="entry name" value="LspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001872">
        <property type="entry name" value="Peptidase_A8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33695">
        <property type="entry name" value="PTHR33695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01252">
        <property type="entry name" value="Peptidase_A8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00781">
        <property type="entry name" value="LIPOSIGPTASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00077">
        <property type="entry name" value="lspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00855">
        <property type="entry name" value="SPASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS85031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="144" mass="16183" checksum="7870F1E9F39C003A" modified="2012-04-18" version="1">MYYGGLVILLVLADQTIKWWAASNLQLGDTITLIPGWLALTNVHNNGAAWSMLEGQHTFFIVIALVAIGVILWLMKRNWHQRAKMVALSLLLAGTLGNFIDRFAQGYVVDMFELTFINFPIFNLADSCLTLGVLIYIVIILRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKW5</accession>
    <name>H4GKW5_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cell cycle protein GpsB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Guiding PBP1-shuttling protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">gpsB</name>
        <name evidence="7" type="ORF">PS3_14619</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Divisome component that associates with the complex late in its assembly, after the Z-ring is formed, and is dependent on DivIC and PBP2B for its recruitment to the divisome. Together with EzrA, is a key component of the system that regulates PBP1 localization during cell cycle progression. Its main role could be the removal of PBP1 from the cell pole after pole maturation is completed. Also contributes to the recruitment of PBP1 to the division complex. Not essential for septum formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Forms polymers through the coiled coil domains. Interacts with PBP1, MreC and EzrA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="6">Shuttles between the lateral wall and the division site in a cell cycle-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GpsB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000072">
        <property type="protein sequence ID" value="EHS85035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_14619"/>
    <dbReference type="EnsemblBacteria" id="EHS85035">
        <property type="protein sequence ID" value="EHS85035"/>
        <property type="gene ID" value="PS3_14619"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1701"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02011">
        <property type="entry name" value="GpsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011229">
        <property type="entry name" value="Cell_cycle_GpsB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019933">
        <property type="entry name" value="DivIVA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007793">
        <property type="entry name" value="DivIVA_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35794">
        <property type="entry name" value="PTHR35794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35794:SF1">
        <property type="entry name" value="PTHR35794:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05103">
        <property type="entry name" value="DivIVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029938">
        <property type="entry name" value="UCP029938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03544">
        <property type="entry name" value="DivI1A_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 6 7" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 6" id="KW-0133">Cell shape</keyword>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="37"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS85035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="121" mass="13672" checksum="742BC8CC4C26E7FD" modified="2012-04-18" version="1">MLILDNINFTPQDILQKQFQEKSIGKGYDREDVDSYLDDIIKDYNTFEKEINRLQDENARLKTKVDELTRQVEAGGSGVATGDAQPLSGATNVDILKRLSNLERRVFGSQLNNGDEDSHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKZ2</accession>
    <name>H4GKZ2_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Poly(Glycerol-phosphate) alpha-glucosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_13749</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122724.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_13749"/>
    <dbReference type="EnsemblBacteria" id="EHS84758">
        <property type="protein sequence ID" value="EHS84758"/>
        <property type="gene ID" value="PS3_13749"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1735"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022372">
        <property type="entry name" value="Accessory_SS_Asp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16993">
        <property type="entry name" value="Asp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="323"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="497" mass="56911" checksum="EF66CAE03DB1C6DE" modified="2012-04-18" version="1">MFFFVNRYLLPRNSSIEHTELARLRLFKRHQSPAQLVLRDFLPNLDQTLQRFDLDRSQVTTLYDFFAGTQDYVGAPLKIDDLALPVDYQIGTGNNFRQVTLGGRLVAEIYFIGGTVAQIGRIDYFDDSGNKTLSQVYDWRGFKCLDRSYGKQGELFYEQYYRPNGQRYLERYYVQSTKNTAINSLNILKDYQGHDYFFDNLDDLFTFFLDELNRSHGENNQFIADRPQETINPVLRMQTKAHKYLWMAMPEGGDRPDQAHGPLATLLQAALVSNVKAWDGILAASQEQVTNLRARVGNQVPVYQVSPVVIKSVAVTEPKTPADFEMIYVGRIAPDKQIDQLLQIFAQIHQQVSKTHLTIYGYCSDQLRRGLDQLIEELKLQGAVTWAGYQVDLSKAYQEADLYLDASRIDAFPLAMVEALAHSVPVVSYDFQYGPKELVQSGVNGELVPVNQVSQFVQKVVQLLKNPAKLNQYRQQAGAQLANCQDELVWQQWERLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="19" xmlns="http://uniprot.org/uniprot">
    <accession>H4GKZ4</accession>
    <name>H4GKZ4_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_5714</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000077">
        <property type="protein sequence ID" value="EHS84760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122726.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000077.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5714"/>
    <dbReference type="EnsemblBacteria" id="EHS84760">
        <property type="protein sequence ID" value="EHS84760"/>
        <property type="gene ID" value="PS3_5714"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1737"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="127" mass="15309" checksum="D51FC0CC0E1F5564" modified="2012-04-18" version="1">MQNPFGNNNQDFFNNLPIPPNYAKIINDQGDIRIAKVGFSWTTLWFGFFPAIFRGDWYNFLVMLFIDADYYLVSVVFGWQWMFALPFPAIFFGFVYNYLYFRHLFHKGYRPADQHSQEVLTRAKYWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL58</accession>
    <name>H4GL58_9LACO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PS3_5747</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">PS3</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122790.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_5747"/>
    <dbReference type="EnsemblBacteria" id="EHS84688">
        <property type="protein sequence ID" value="EHS84688"/>
        <property type="gene ID" value="PS3_5747"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1815"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS84688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="160" mass="18542" checksum="FFF23A7725FD0F18" modified="2012-04-18" version="1">MSFLYVITITLILILVIYQIIHRTLVKRHDQQQQVAAQQITDQVVMAVLADHLASDHYDHQSSIVAEVWGNGVQAFEYQIYFDLCDEQELRWLSHQNLSQAIEKNASQELIAGFNAQSAPFVVTDWWVYQQKLHFDVAYVSNEATREYVEDLSKLNQKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-08-03" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL82</accession>
    <name>H4GL82_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative tRNA (cytidine(34)-2'-O)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.207</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA (cytidine/uridine-2'-O-)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PS3_3796</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could methylate the ribose at the nucleotide 34 wobble position in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-carboxymethylaminomethyluridine(34) in tRNA(Leu) + S-adenosyl-L-methionine = 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu) + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43088"/>
            <dbReference type="Rhea" id="RHEA-COMP:10333"/>
            <dbReference type="Rhea" id="RHEA-COMP:10334"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74508"/>
            <dbReference type="ChEBI" id="CHEBI:74511"/>
            <dbReference type="EC" id="2.1.1.207"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cytidine(34) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(34) in tRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43084"/>
            <dbReference type="Rhea" id="RHEA-COMP:10331"/>
            <dbReference type="Rhea" id="RHEA-COMP:10332"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.207"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.207" evidence="1"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122814.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_3796"/>
    <dbReference type="EnsemblBacteria" id="EHS84712">
        <property type="protein sequence ID" value="EHS84712"/>
        <property type="gene ID" value="PS3_3796"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1848"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18094">
        <property type="entry name" value="SpoU-like_TrmL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01885">
        <property type="entry name" value="tRNA_methyltr_TrmL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016914">
        <property type="entry name" value="tRNA_cyt/urid_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42971">
        <property type="entry name" value="PTHR42971"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029256">
        <property type="entry name" value="SpoU_TrmH_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SpoU_methylase" evidence="3">
        <location>
            <begin position="4"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR029256-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS84712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="169" mass="19462" checksum="891D221CC8CEA57D" modified="2012-04-18" version="1">MTNHIVLFEPLFPANTGNIARTCAGTNTVLHLIKPLGFSTDDKHMKRAGLDYWDKVEVVYHDDLPSFMATLPDLENFYIVSKFAERDYSQADYMTAGDHYFLFGKETTGLPEPFMRQYPDQAIRIPQNDDHIRALNLSNSAAIVIYEAMRQQGFPQLARTHRYEDDKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2022-01-19" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>H4GL85</accession>
    <name>H4GL85_9LACO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PS3_6535</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Limosilactobacillus gastricus PS3</name>
        <dbReference type="NCBI Taxonomy" id="1144300"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Firmicutes</taxon>
            <taxon>Bacilli</taxon>
            <taxon>Lactobacillales</taxon>
            <taxon>Lactobacillaceae</taxon>
            <taxon>Limosilactobacillus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00489" last="E00413">
            <title>Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk.</title>
            <authorList>
                <person name="Martin V."/>
                <person name="Cardenas N."/>
                <person name="Jimenez E."/>
                <person name="Maldonado A."/>
                <person name="Rodriguez J.M."/>
                <person name="Fernandez L."/>
            </authorList>
            <dbReference type="PubMed" id="23846278"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">PS3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Responsible for synthesis of pseudouridine from uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a uridine in RNA = a pseudouridine in RNA</text>
            <dbReference type="Rhea" id="RHEA:48348"/>
            <dbReference type="Rhea" id="RHEA-COMP:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:12069"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="AICN01000078">
        <property type="protein sequence ID" value="EHS84715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_007122817.1">
        <property type="nucleotide sequence ID" value="NZ_AICN01000078.1"/>
    </dbReference>
    <dbReference type="STRING" id="1144300.PS3_6535"/>
    <dbReference type="EnsemblBacteria" id="EHS84715">
        <property type="protein sequence ID" value="EHS84715"/>
        <property type="gene ID" value="PS3_6535"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1144300.3.peg.1851"/>
    <dbReference type="Proteomes" id="UP000004567">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140098">
        <property type="term" value="F:catalytic activity, acting on RNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034470">
        <property type="term" value="P:ncRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PseudoU_synth_2" evidence="4">
        <location>
            <begin position="86"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS84715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000004567"/>
        </source>
    </evidence>
    <sequence length="301" mass="34160" checksum="F4E900FA2F5685C5" modified="2012-04-18" version="1">MRFDWTYRGKYPRRLRAVLKSFGVSSSLIKVAIFHGGQMQINNHPQWAVAQVQPGDQVSLLLPDEPANPNLDVSDEPLEIVYEDRDFLVVNKPAGVATVPAHNVPVHNSLVNRLKGYYLRQQYPNQVTHVATRLDRDTSGLVVFPKHRFAHAVLDQQLKQHQVTKRYLAIVQGKVPAKHGVIMAPIRRDPASFVQRQVARDGKMSITEYWAQEMTDAKTVVRIALHSGRTHQIRVHFQSIGHPLIGDDMYGQASPLINRQALHCQYVSFYSPFINQVIEVSVPIPADMRNLIQKNEAGRNF</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>